| oxidation-reduction properties of chromatium vinosum high potential iron-sulfur protein. | the oxidation-reduction properties of the high potential iron-sulfur protein (hipip) from chromatium vinosum have been investigated. both equilibrium and kinetic measurements demonstrate electron transport by hipip is ph independent in the ph range 7-11. the kinetics of reduction (potassium ferrocyanide, so2, s2o42-, sodium ascorbate, and rhodospirillum rubrum cytochrome c2) and oxidation (potassium ferricyanide and rhodospirillium rubrum cytochrome c2) of hipip are reported. based on the data o ... | 1976 | 2285 |
| photoinduced reduction of nad(p) in the cells of green sulfur bacteria. | the spectrum of a photoinduced increase in luminescence of the cells of the gree sulphur bacterium chlorobium limicola f. thiosulfatophilum, within the range of 400 to 520 nm, was found to correspond to the spectrum of luminescence of nadh in the protein-bound form. photoinduced reduction of nad(p) in green bacteria, contrary to purple bacteria, is not susceptible to the action of p-chlorocarbonylcyanide phenlhydrazone which uncouples photophosphorylation. therefore, in chlorobium limicola f. th ... | 1975 | 2843 |
| the primary acceptor of bacterial photosynthesis: its operating midpoint potential? | | 1976 | 4013 |
| mechanism of inhibition of chromatium d growth by l-methionine. regulation of l-threonine biosynthesis by the intracellular level of s-adenosylmethionine. | (1) an unusual accumulation of s-adenosyl-l-methionine in chromatium d was associated with a marked growth inhibition by l-methionine. the inhibition was overcome by l-isoleucine, l-leucine, l-phyenylalanine, l-threonine, l-valine and putrescien. based on their effects, these compounds are classified into 3 types. (2) l-isoleucine, l-leucine, l-phyenylalanine and l-valine (type i) inhibited the l-methionine uptake and consequently prevented the bacterium from the unusual accumulation of s-adenos ... | 1976 | 8102 |
| on the ecology of marine chromatiaceae and chlorobiaceae (author's transl). | number and genera (types) of phototrophic red and green sulfur bacteria have been determined in the upper layer of eleven marine sediment samples from the euphotic zone in the mediterranean sea. origin and nature of the sediment samples are given as well as their ph, total nitrogen and sulfide content. the results show that the sediments studied did not provide good growth conditions for chromatiaceae (chromatium, thiocystis, thiocapsa) and chlorobiaceae (prosthecochloris). the total number of t ... | 1976 | 9896 |
| magnetic studies of chromatium flavocytochrome c552. a mechanism for heme-flavin interaction. | electron paramagnetic resonance and magnetic susceptibility studies of chromatium flavocytochrome c552 and its diheme flavin-free subunit at temperatures below 45 degrees k are reported. the results show that in the intact protein and the subunit the two low-spin (s = 1/2) heme irons are distinguishable, giving rise to separate epr signals. in the intact protein only, one of the heme irons exists in two different low spin environments in the ph range 5.5 to 10.5, while the other remains in a con ... | 1976 | 9997 |
| fermentation of pyruvate by 7 species of phototrophic purple bacteria. | the dark, anaerobic fermentation of pyruvate under growth conditions was examined with the following species of phototrophic purple bacteria: rhodospirillum rubrum strains ha and s1, rhodopseudomonas gelatinosa strain 2150, rhodopseudomonas acidophila strain 7050, rhodopseudomonas palustris strain atcc 17001, rhodopseudomonas capsulata strains kb1 and 6950, rhodopseudomonas sphaeroides strain atcc 17023, and chromatium vinosum strain d. fermentation balances were established for all experiments. ... | 1976 | 12621 |
| delayed fluorescence from bacteriochlorophyll in chromatium vinosum chromatophores. | delayed fluorescence from bacteriochlorophyll in chromatium vinosum chromatophores was studied at room temperature and under intermittent illuminations. the decay of delayed fluorescence was constituted of two components; a fast component decayed with a half time of about 8 ms, a slow one decayed in parallel with the reduction of photooxidized bacteriochlorophyll (p+) with a half time of 100-200 ms. the biphasic decay of delayed fluorescence indicated that a rapid equilibrium was established bet ... | 1977 | 12813 |
| kinetic study of the dissolution of fe4s4(2-)-cluster core ions of ferredoxins and high potential iron protein. | | 1977 | 18167 |
| utilization of nitrogen compounds and ammonia assimilation by chromatiaceae. | chromatium vinosum strain d, thiocapsa roseopersicina strain 6311 and ectothiorhodospira mobilis strain 8112 were grown anaerobically in the light with various single nitrogen sources. when substituted for nh4cl only glutamine and casamino acids supported good growth of all strains tested. peptone and urea were utilized by c. vinosum and t. roseopersicina, glutamate, asparagine and nitrate only by c. vinosum. the strains were able to grow with molecular nitrogen; complete inhibition of this grow ... | 1977 | 19002 |
| dependence of the rates of dissolution of the fe4s4 clusters of chromatium vinosum high-potential iron protein and ferredoxin on cluster oxidation state. | the influence of oxidation state on the ph dependence of the dissolution of the fe(4)s(4) clusters of chromatium vinosum ferredoxin and high-potential iron protein (hipip) has been studied. the first-order rate constants (k(obs)) for dissolution of both the fe(4)s(4)(s-cys)(4) (2-) and fe(4)s(4)(s-cys)(4) (3-) clusters of the ferredoxin follow the same overall kinetic equation but with differing specific rate and equilibrium constants. the dependence of rate and equilibrium constants upon oxidat ... | 1977 | 23530 |
| active transport in the photosynthetic bacterium chromatium vinosum. | | 1978 | 30400 |
| regulation of electron transfer in chromatium vinosum chromatophores by intravesicular h+ concentration. | | 1979 | 33164 |
| manganese, an essential trace element for n2 fixation by rhodospirillum rubrum and rhodopseudomonas capsulata: role in nitrogenase regulation. | nitrogenase (n(2)ase) from the photosynthetic bacterium rhodospirillum rubrum can exist in two forms, an unregulated form (n(2)ase a) and a regulatory form (n(2)ase r), the latter being identified in vitro by its need for activation by a mn(2+)-dependent n(2)ase activating system. the physiological significance of this mn(2+)-dependent n(2)ase activating system was suggested here by observations that growth of r. rubrum and rhodopseudomonas capsulata on n(2) gas (a condition that produces active ... | 1979 | 42641 |
| energetic coupling in the primary processes of photosynthesis in chromatium. ph dependence of delayed fluorescence, electron transfer and degree of coupling. | the effects of ph on the thermodynamic properties of the proton-translocating cyclic electron transfer system in a purple photosynthetic bacterium chromatium vinosum were studied. two thermodynamic parameters, the flux (je) and force (deltamue) of the electron transfer process, were analyzed. the rate of electron transfer in the re-reduction of photooxidized reaction-center bacteriochlorophyll was used as je. deltamue was determined from the intensity of the delayed fluorescence from bacteriochl ... | 1979 | 84810 |
| flash-induced changes in the in vivo bacteriochlorophyll fluorescence yield at low temperatures and low redox potentials in carotenoid-containing strains of photosynthetic bacteria. | the changes in the in vivo bacteriochlorophyll fluorescence induced by a xenon flash at low temperatures (77--200 k) with the "primary" acceptor x chemically prereduced have been examined in whole cells of several species of photosynthetic bacteria which contain carotenoids absorbing in the visible part of the absorption spectrum. two groups of species with different behaviour could be distinguished. in both cases a flash-induced rise of the fluorescence yield was observed with x prereduced at 7 ... | 1978 | 96856 |
| nitrogen fixation and hydrogen metabolism in photosynthetic bacteria. | the photosynthetic bacteria are found in a wide range of specialized aquatic environments. these bacteria represent important members of the microbial community since they are capable of carrying out two of the most important processes on earth, namely, photosynthesis and nitrogen fixation, at the expense of solar energy. since the discovery that these bacteria could fix atmospheric nitrogen, there has been an intensification of studies relating to both the biochemistry and physiology of this pr ... | 1978 | 96875 |
| dichroism of bacteriochlorophyll in chromatophores of photosynthetic bacteria. | the dichroism was measured in films of air-dried and, consequently, flattened chromatophores of chromatium vinosum, rhodopseudomonas sphaeroides and rhodospirillum rubrum. the values (deltaa/a) of dichroism in c. vinosum were found to be -1.05 at 590 nm and 0.75 in the near infrared region. the values of dichroism in r. sphaeroides were -0.70 at 590 nm and 0.80 at 870 nm. the values of dichroism in r. rubrum were -1.45 at 590 nm and 0.97 at 870 nm. | 1978 | 97281 |
| oxonol dyes as monitors of membrane potential. their behavior in photosynthetic bacteria. | the reponses of oxonol dyes to single and multiple single turnovers of the photosynthetic apparatus of photosynthetic bacteria have been studied, and compared with the responses of the endogenous carotenoid pigments. the absorbance changes of the oxonols can be conveniently measured at 587 nm, because this is an isosbestic point in the 'light-minus-dark' difference spectrum of the chromatophores. the oxonols appear to respond to the light-induced 'energization' by shifting their absorption maxim ... | 1979 | 103582 |
| ribulose bisphosphate carboxylases from chromatium vinosum and rhodospirillum rubrum and their role in photosynthetic carbon assimilation. | | 1978 | 106836 |
| the preparation and characterization of different types of light-harvesting pigment-protein complexes from some purple bacteria. | a general strategy, with some specific examples, is given for the isolation and purification of detergent-soluble, antenna pigment-protein complexes from the photosynthetic membranes. absorption, fluorescence and circular dichroism spectra, and the pigment and protein composition of b800-b850-protein and b890-protein complexes of some purple bacteria (rhodospirillum rubrum, rhodopseudomonas sphaeroides and rps. capsulata and chromatium vinosum) are discussed. we conclude that there are probably ... | 1978 | 110568 |
| [use of urea by purple bacteria]. | strains of purple sulfur bacteria (chromatium minutissimum, ectothiorhodospira shaposhnikovii, thiocapsa roseopersicina, lamprobacter modestohalophilus) and nonsulfur bacteria (rhodopseudomonas palustris, rh. spheroides, rhodospirillum rubrum) grow in media containing urea as a source of nitrogen at concentrations from 0.5 to 5.0%. they can also utilize the carbon of urea and thus grow in the absence of bicarbonate. urea is decomposed by all the studied purple bacteria with the participation of ... | 1979 | 112359 |
| lipopolysaccharides of photosynthetic prokaryotes. | | 1979 | 115382 |
| [enzymes of carbohydrate metabolism in phototrophic bacteria]. | purple sulphur bacteria (chromatium minutissimum, ectothiorhodospira shaposhnikovii, thiocapsa roseopersicina), non-sulphur bacteria (rhodopseudomonas palustris rh. viridis), and green sulphur bacteria (chlorobium limicola f. thiosulfatophillum) contain all enzymes of the fructose diphosphate pathway of carbohydrate transformation, and also glucose-6-phosphate dehydrogenase. the activity of fructose diphosphate aldolase, triose phosphate dehydrogenase, and glucose-6-phosphate dehydrogenase incre ... | 1975 | 125844 |
| proceedings: properties of adenosine triphosphatase in photosynthetic organisms. | | 1975 | 128539 |
| coupling factors atpases from photosynthetic bacteria. | | 1976 | 134033 |
| properties of atpase activity in coupling factor from chromatium strain d chromatophores. | coupling factor extracted from chromatophores of the photosynthetic bacteria chromatium strain d was partially purified. the enzyme catalyzed atpase activity in the presence of ca2+ and mg2+ ions. higher vapp values were obtained when the activity was measured as a function of the divalent cation-atp complex rather than as a function of either the divalent cation or atp because the free components competitively inhibited the activity in the presence of the cation-atp complex. the km values were ... | 1977 | 140048 |
| purification and properties of adenosine triphosphatase from chromatium vinosum chromatophores. | | 1978 | 145959 |
| the nitrogen-fixing complex of bacteria. | | 1975 | 164247 |
| the iron electron-nuclear double resonance (endor) of 4-fe clusters in iron-sulfur proteins from chromatium and clostridium pasteurianum. | iron electron-nuclear double resonance (endor) measurements were made of the 4-fe clusters in oxidized chromatium high-potential iron-sulfur protein, dithionite-reduced high-potential iron-sulfur protein in 80% dimethylsulphoxide, fully reduced clostridium pasteurianum ferredoxin in aqueous solution and in 80% dimethylsulfoxide. the hyperfine couplings determined show that: i) the electron distribution in each case is nearly symmetric; ii) there are two types of iron in oxidized high potential i ... | 1975 | 164903 |
| ribulose-5-phosphate kinase from chromatium. | | 1975 | 166272 |
| immunological comparison of ferredoxins. | | 1975 | 166874 |
| nature of photochemical reactions in chromatophores of chromatium d. iii. heterogeneity of the photosynthetic units. | the effect of isooctane extraction on photooxidation of c-type cytochromes was investigated in chromatium chromatophores. photooxidation of cytochrome c-555 was not affected by isooctane-extraction was abolished by thorough extraction of ubiquinone-7, but the quantum yield of the cytochrome photooxidation remained unchanged until 90% of the total ubiquinone was extracted. the photooxidation of cytochrome c-552 was recovered by the addition of ubiquinone-7 but not by menaquinone. a dark incubatio ... | 1975 | 167850 |
| evolution of photosystems of photosynthetic organisms. | it is generally accepted that two photosystems function successively in photosynthetic electron transport chain of plants and algae. the interaction of these photosystems results in the enhancement of photosynthesis. it was suggested that only one photosystem is present in purple bacteria, the most primitive photosynthetic organisms. the functioning of this photosystem is accompanied by absorption changes at 890 nm. recently new spectral changes were found in chramatium chromatophores under redu ... | 1975 | 168538 |
| [primary processes in energy transfer of photosynthesis (author's transl)]. | | 1975 | 169548 |
| high and low reduction potential 4fe-4s clusters in azotobacter vinelandii (4fe-4s) 2ferredoxin i. influence of the polypeptide on the reduction potentials. | azotobacter vinelandii (4fe-4s)2 ferredoxin i (fd i) is an electron transfer protein with mr equals 14,500 and eo equals -420 mv. it exhibits and epr signal of g equals 2.01 in its isolated form. this resonance is almost identical with the signal that originates from a "super-oxidized" state of the 4fe-4s cluster of potassium ferricyanide-treated clostridium ferredoxin. a cluster that exhibits this epr signal at g equals 2.01 is in the same formal oxidation state as the cluster in oxidized chrom ... | 1975 | 170272 |
| magnetic studies of the four-iron high-potential, non-heme protein from chromatium vinosum. | extensive epr studies on high-potential, iron-sulfur protein from chromatium vinosum indicate that the singular spectrum of this four-iron, non-heme protein consists of a superposition of three distinct signals; namely, two principal signals of equal weight, one reflecting axial and the other rhombic symmetry, and a third nearly isotropic minority component. in addition, magnetic susceptibility experiments on two oxidation states of the protein from 4.2 to approx. 260 degrees k indicate antiferr ... | 1975 | 170982 |
| kinetic studies of the oxidation and reduction of chromatium high potential iron-sulfur protein (hipip) by inorganic complexes. comparison of the electron transfer reactivities of hipip and horse heart cytochrome c. | | 1976 | 176192 |
| oxidation-reduction properties of several low potential iron-sulfur proteins and of methylviologen. | apparent oxidation-reduction potentials at ph 7.0 and 25 degrees c were determined using the h2-hydrogenase system with ferredoxins from the following sources: clostridium pasteurianum, -403 mv; c tartarovorum, -424 mv; c. acidi-urici, -434 mv; peptococcus aerogenes, -427 mv; chromatium d, -482 mv (ph 8.0); b. polymyxa, fd i, -377 mv, and fd ii, -422 mv; and spinach, -428 mv. the ph dependence of these values was variable, ranging from -2 to -24 mv/ph unit increase for different ferredoxins. ove ... | 1976 | 181047 |
| visible absorption spectra of quantum mixed-spin ferric heme proteins. | recently, it has been shown that the magnetic data for chromatium ferricytochrome c' at ph 7 are consistent with quantum mechanically (as distinguished from thermally) mixed mid-spin (s = 3/2) and high-spin (s = 5/2) heme. visible absorption spectra of the protein measured at 77 degrees k and 293 degrees k, ph 7, show peaks at 400, 490, and 632 nm. the observation of a 630 nm band in quantum mixed-spin heme spectra, and the spin state-dependence of the band intensity, are discussed in the contex ... | 1976 | 182223 |
| epr properties of the electron carrier intermediate between the reaction center bacteriochlorophylls and the primary acceptor in chromatium vinosum. | | 1976 | 182533 |
| the primary photoreactions in the complex cytochrome-p-890-p-760 (bacteriopheophytin760) of chromatium minutissimum at low redox potentials. | experimental evidence for electron transfer, photosensitized by bacteriochlorophyll, from cytochrome c to a pigment complex p-760 (involving bacteriopheophytin-760 and also bacteriochlorophyll-800) in the reaction centers of chromatium minutissimum has been described. this photoreaction occurs between 77 and 293 degrees k at a redox potential of the medium between -250 and -530 mv. photoreduction of p-760 is accompanied by development of a wide absorption band at 650 nm and of an epr signal with ... | 1976 | 183814 |
| epr and optical spectroscopic properties of the electron carrier intermediate between the reaction center bacteriochlorophylls and the primary acceptor in chromatium vinosum. | 1. a reaction center-cytochrome c complex has been isolated from chromatium vinosum which is capable of normal photochemistry and light-activated rapid cytochrome c553 and c555 oxidation, but which has no antenna bacteriochlorophyll. as is found in whole cells, ferrocytochrome c553 is oxidized irreversibly in milliseconds by light at 7 k. 2. room temperature redox potentiometry in combination with epr analysis at 7 k, of cytochrome c553 and the reaction center bacteriochlorophyll dimer (bchl)2 a ... | 1976 | 187221 |
| near-infrared magnetic circular dichroism of cytochrome c'. | the near-infrared magnetic circular dichroism (mcd) of rhodospirillum rubrum, chromatium vinosum, and rhodopseudomonas palustris cytochromes c' are reported. the spectra of the reduced protein are very similar to those of deoxymyoglobin. the spectra of the oxidized proteins in the pd range 1-13 can be analyzed on the basis of four species a, b, c, and d. the existence of nine species, reported in a recent electron paramagnetic resonance study, is not substantiated. the mcd spectra support the as ... | 1977 | 192272 |
| enzymic formation of glycolate in chromatium. role of superoxide radical in a transketolase-type mechanism. | chromatophores prepared from chromatium exhibit a light-dependent o2 uptake in the presence of reduced 2,6-dichlorophenolindophenol, the maximum rate observed being 10.8 micronmol (mg of bchl)-1 h-1 (air-saturated condition). as it was found that the uptake of o2 was markedly inhibited by superoxide dismutase, it is suggested that molecular oxygen is subject to light-dependent monovalent reduction, resulting in the formation of the superoxide anion radical (o2-). by coupling baker's yeast transk ... | 1977 | 193557 |
| evidence for a thioether linkage between the flavin and polypeptide chain of chromatium cytochrome c 552. | | 1977 | 194892 |
| electron-transfer protein reactivities. kinetic studies of the oxidation of horse heart cytochrome c, chromatium vinosum high potential iron-sulfur protein, pseudomonas aeruginosa azurin, bean plastocyanin, and rhus vernicifera stellacyanin by pentaamminepyridineruthenium(iii). | | 1977 | 194940 |
| function and properties of a soluble c-type cytochrome c-551 in secondary photosynthetic electron transport in whole cells of chromatium vinosum as studied with flash spectroscopy. | | 1977 | 196641 |
| electron paramagnetic resonance determination of a low-lying excited state in chromatium vinosum high-potential iron protein. | the temperature dependence of the epr spectrum of oxidized high-potential iron protein from chromatium vinosum has been studied. from line width and intensity measurements it is possible to determine the position of the first excited unoccupied state, 160 +/- 10 cm-1 above the ground state orbital. | 1977 | 198036 |
| [comparative study of b890 pigment-lipoprotein complexes from sulfur (chromatium minutissimum) and non-sulfur (rhodopseudomonas palustris) purple photosynthesizing bacteria]. | pigment-lipoprotein b890 complexes containing reaction center and "light-focusing" bacteriochlorophyll a were isolated from photosynthetic membranes of sulfur (chromatium minutissimum) and non-sulfur (rhodopseudomonas palustris) purple bacteria after the treatment with triton x-100. the molecular weights of complexes were evaluated using several methods (200 000-300 000). by means of electron microscopy the sizes of complexes were found to be about 80 a. on the air-water interface hexagonal pack ... | 1978 | 207362 |
| structural organization of the chromatium vinosum reaction center associated c-cytochromes. | magnetic interactions operating between the chromatium vinosum reaction center associated c-cytochromes and the electron carriers of the reaction center have been assayed by comparing the magnetic properties of these components alone, and in various combinations with paramagnetic forms of the reaction center electron carriers. these studies have yielded the following results. 1. the oxidized paramagnetic forms of the high potential cytochromes c-555 produce no discernable alteration of the light ... | 1978 | 210808 |
| [epr study of redox titration of chromatium minutissimum chromatophores]. | redox titration of chromatophores through the region +300 +570 mv at room temperature results in generation of two dark esr signals, a well-known reaction center signal and a new narrow line. this line is characterized by deltah pp = 4.2 oe, g-factor about that of rc and a lorentzian shape. it is insensitive to illumination. the determination of an rc signal midpoint potential is affected by the presence of the narrow line. taking into account the superposition of the two esr signals it was meas ... | 1978 | 212119 |
| identification of iron--sulfur clusters in proteins. | | 1978 | 213682 |
| isolation and characterization of a membrane-bound, low-potential c-type cytochrome from purple photosynthetic bacteria, with special reference to rhodospirillum rubrum. | other investigators have isolated soluble, low-potential, c-type cytochromes (cytochrome c3) from a few photosynthetic procaryotes, i.e., a cyanobacterium and two species of purple nonsulfur bacteria. however, such cytochromes appeared to be absent from other purple bacteria, including rhodospirillum rubrum and chromatium vinosum. we now report evidence for the presence of low-potential c-type cytochromes in these two species, in which they were found to be bound to the photosynthetic membranes. ... | 1978 | 214418 |
| electron spin relaxation of iron-sulphur proteins studied by microwave power saturation. | the electron-spin relaxation of iron-sulphur centres in a range of simple proteins (ferredoxin, high-potential iron-sulphur protein and rubredoxin) was investigated by means of the temperature dependence and microwave power saturation of the epr signal. the proteins containing [2fe-2s] centres all showed temperature optima higher than those for [4fe-4s] centres, but the difference between the slowest-relaxing [4fe-4s] protein (chromatium high-potential iron-sulphur protein) and the fastest-relax ... | 1978 | 215217 |
| a partial purification of membrane-bound b and c cytochromes from chromatium vinosum. | | 1979 | 219774 |
| the amino acid sequence of cytochrome c' from the purple sulphur bacterium chromatium vinosum. | an amino acid sequence is proposed for the cytochrome c' from the photosynthetic purple sulphur bacterium chromatium vinosum strain d. it is single polypeptide chain of 131 residues, with haem-attachment cysteine residues at positions 121 and 124. the results discredit an earlier report [dus, bartsch & kamen (1962) j. biol. chem 237, 3083--3093] of a di-haem peptide sequence from this protein. the sequence belongs to the same class as the published alcaligenes and rhodospirillum rubrum cytochrom ... | 1979 | 220951 |
| electron transfer in the photosynthetic reaction center. | | 1976 | 222400 |
| flavocytochrome c of chromatium vinosum. some enzymatic properties and subunit structure. | the function and the structural features of chromatium vinosum cytochrome c-552 have been investigated. cytochrome c-552 has a sulfide-cytochrome c reductase activity and also catalyzes the reduction of elementary sulfur to sulfide with reduced benzylviologen as the electron donor. in the sulfide-cytochrome reduction, horse and yeast cytochromes c act as good electron acceptors, but cytochrome c' or cytochrome c-553(550) purified from the organism does not. the subunit structure of cytochrome c- ... | 1979 | 222744 |
| preparation of subunits of flavocytochromes c derived from chlorobium limicola f. thiosulfatophilum and chromatium vinosum. | | 1979 | 227287 |
| [possible pathways for acetyl-coa formation by purple bacteria]. | purple sulfur (ectothiorhodospira shaposhnikovii, chromatium minutissimum, lamprobacter modestohalophilus, thiocapsa roseopersicina) and nonsulfur (rhodospirillum rubrum, rhodopseudomonas palustris, rhodopseudomonas spheroides) bacteria are capable of forming acetyl-coa synthetase, phosphotransacetylase and acetokinase independent of the medium composition and growth conditions. in all of the purple sulfur bacteria with an exception of e. shaposhnikovii, the activity of acetokinase is much highe ... | 1979 | 228168 |
| further studies on the subunit structure of chromatium ribulose-1,5-phosphate carboxylase. | upon alkali exposure chromatium ribulose-1,5-bisphosphate carboxylase dissociates into constituent subunits, a catalytic oligomer of the larger subunit, a8, and monomeric form of the small subunit b. by sedimentation equilibrium molecular weights of the native enzyme and the catalytic oligomer produced by an alkali treatment were estimated to be 5.11 x 10 5 and 4.29 x 10 5, respectively. to provide information on reversibility of the dissociation by determining whether the enzymically inactive s ... | 1975 | 234018 |
| epr studies of protein state transitions in chromatium ferricytochrome c'. | recent magnetic studies have shown that in the ph range 1 to 11 the bacterial heme protein ferricytochrome c' can undergo reversible transitions between various pure high-spin and quantum mechanically mixed (intermediate and high)-spin protein states. the epr data presented here extend the recent work to high alkaline ph and show that above ph 11.6 reversible transitions occur between various pure high-spin and pure low-spin protein states. the new data and the previous magnetic studies of the p ... | 1975 | 234758 |
| the primary photochemical reaction to bacterial photosynthesis. | | 1975 | 235329 |
| the reaction of euglena gracilis cytochrome c-552 with nonphysio-logical oxidants and reductants. | | 1975 | 237478 |
| purification and properties of the glutathione reductase of chromatium vinosum. | the chromatium vinosum glutathione reductase [nad(p)h: glutathione disulfide oxidoreductase, ec 1.6.4.2] was purified to apparent homogeneity. the enzyme was found to require reduced nicotinamide adenine dinucleotide (nadh) as a reductant and to be specific for oxidized glutathione (gssg). the polypeptide molecular weight in sodium dodecyl sulfate was found to be 52,000. incubation of enzyme with nadh in the absence of gssg resulted in a significant loss in activity. the enzyme was stimulated by ... | 1975 | 237878 |
| the acid-base properties and kinetics of dissolution of the fe4s4 cores of chromatin ferredoxin and high potential iron protein. | | 1975 | 238529 |
| structural and catalytic properties of hydrogenase from chromatium. | the enzyme hydrogenase, from the photosynthetic bacterium chromatium, was purified to homogeneity after solubilization of the particulate enzyme with deoxycholate. the purification procedure included ammonium sulfate fractionation, treatment with manganous phosphate gel, heating at 63 degrees, deae-cellulose chromatography, and isoelectric focusing. the last step gave two active enzyme fractions with isoelectric points of 4.2 and 4.4. it was shown that the two fractions were different forms of t ... | 1975 | 238560 |
| the role of sulfhydryl groups in the ribulose- 1,5-bisphosphate carboxylase and oxygenase reactions. | | 1975 | 241298 |
| prediction of beta-turns. | an automated computer prediction of the chain reversal regions of globular proteins is described herein using bend frequencies and beta-turn conformational parameters (pt) determined from 408 beta-turns in 29 proteins calculated from x-ray atomic coordinates. the probability of bend occurrence at residue i is pt = fi x fi+1 x fi+2 x fi+3 with the average bend probability less than pt greater than = 0.55 x 10(-4). tetrapeptides with pt greater than 0.75 x 10(-4) ( approximately to 1.5 x less than ... | 1979 | 262423 |
| chromatium hydrogenase. | previous reports of a relatively air-stable soluble hydrogenase from the photosynthetic anaerobe, chromatium vinosum, have been substantiated. the properties of this enzyme, as seen in highly purified samples prepared by procedures that permit improved yields, are described. a possible role for flavin mediation is noted. | 1977 | 265580 |
| cluster characterization in iron-sulfur proteins by magnetic circular dichroism. | we report magnetic circular dichroism (mcd) spectra of 4-fe iron-sulfur clusters in the iron-sulfur proteins chromatium high-potential iron protein (hipip), bacillus stearothermophilus ferredoxin and clostridium pasteurianum ferredoxin. the mcd is found to vary significantly with cluster oxidation state but is relatively insensitive to the nature of the protein. the spectra obtained are compared with the corresponding spectra of iron-sulfur proteins containing 2-fe clusters. it is concluded tha ... | 1978 | 281679 |
| photochemical reactions centre of photosynthetic bacteria. | | 1979 | 317049 |
| formation of cobalt-free corrinoids in rhodopseudomonas spheroides. | co-free corrinoids (cfcs) were found in the cells of rhodopseudomonas spheroides grown without cobalt salt. a predominant fraction of three cfcs on paper-electrophoregram was further purified, and investigated physico-chemically as well as microbiologically in comparison with that isolated by toohey from chromatium. the electrophoretic as well as spectrophotometric patterns of cfc from rh. spheroides were partly different from those of chromatium cfc. the former cfc was a competitive inhibitor o ... | 1977 | 335029 |
| [oxygen effect in photosynthetic organisms (author's transl)]. | | 1977 | 337402 |
| rapid sampling culture chamber. | an all-glass chamber for culturing anaerobic and aerobic bacteria in liquid medium is described. the system permits both rapid sampling and turbidimetric measurements under controlled atmospheric conditions. | 1978 | 350159 |
| mechanisms of co2 fixation in bacterial photosynthesis studied by the carbon isotope fractionation technique. | the carbon isotope discrimination properties of a representative of each of the three types of photosynthetic bacteria chlorobium thiosulfatophilum, rhodospirillum rubrum and chromatium and of the c3-alga chlamydomonas reinhardii were determined by measuring the ratio of 13co2 to 12co2 incorporated during photoautotrophic growth. 2. chromatium and r. rubrum had isotope selection properties similar to those of c3-plants, whereas chlorobium was significantly different. 3. the results suggest that ... | 1977 | 402896 |
| reduction of adenylylsulfate and 3'-phosphoadenylylsulfate in phototrophic bacteria. | extracts of 14 species of phototrophic bacteria, partly grown with different sulfur compounds, were tested for their ability to form volatile sulfur compounds from adenylylsulfate (aps) and 3'-phosphoadenylylsulfate (paps). the rhodospirillum species showed marked activities with both aps and paps while the rhodopseudomonas species seem to prefer paps. the chromatiaceae exhibited the strongest activities with aps, whereas chlorobium limicola had equally high activity with paps. | 1977 | 408177 |
| [carbonic anhydrase activity of phototropic bacteria]. | the activity of carboanhydrase was assayed in seven species of phototrophous bacteria: three species of the rhodospirillaceae genus, three species of the chromatiaceae genus, and one species of the chlorobiaceae genus. the activity of carboanhydrase was found in five species among seven. it decreased on passing from photoautotrophous to photoheterotrophous conditions of growth, and then to dark heterotrophous conditions, which correlated with the activity of the calvin cycle in the bacteria. no ... | 1977 | 408582 |
| preparation of chlorophyll a, chlorophyll b and bacteriochlorophyll a by means of column chromatography with diethylaminoethylcellulose. | | 1978 | 413574 |
| determination of glycolic acid by the eegriwe (calkins) methods. | | 1977 | 414636 |
| location of o-methyl sugars in antigenic (lipo-)polysaccharides of photosynthetic bacteria and cyanobacteria. | an attempt was made to localize a number of o-methyl sugars in lipopolysaccharides and antigenic polysaccharides isolated from photosynthetic bacteria and from cyano-bacteria. methylation analysis with [2h3]methyl iodide as methylating agent was the method of choice. one has to differentiate between (lipo-)polysaccharides having only trace amounts (less than 1% of polymer dry weight) of o-methyl sugars and those having them in larger amounts (more than 4% of polymer dry weight). in the former ca ... | 1978 | 418776 |
| the light-reaction of the green photosynthetic bacterium chlorobium limicola f. thiosulfatophilum at cryogenic temperatures. | | 1979 | 421901 |
| the role of the membrane potential in active transport by the photosynthetic bacterium chromatium vinosum. | | 1979 | 428553 |
| modifiable chromatophore proteins in photosynthetic bacteria. | the chromatophores of chromatium vinosum, as well as six other photosynthetic bacteria, contained two or more proteins which were insoluble when heated in the presence of sodium dodecyl sulfate (sds) and 2-mercaptoethanol (beta-me). when the chromatophores were dissolved at room temperature in sds-beta-me, these proteins were present in the sds-polyacrylamide gel electrophoresis profiles, but when the samples were dissolved at 100 degrees c, they were absent or considerably diminished. when one- ... | 1979 | 438130 |
| the energy-linked carotenoid band-shift in chromatium vinosum. | | 1979 | 464603 |
| separation and purification of two hydrogenase activities in chromatium: kinetics and subunit analysis [proceedings]. | | 1979 | 510723 |
| properties of the monomeric and dimeric forms of chromatium hydrogenase [proceedings]. | | 1979 | 510724 |
| studies on the surface structure of the intracytoplasmic membrane in the photosynthetic purple bacterium chromatium vinosum by means of chemical modification. | by means of a chemical modification technique, the surface structure of the intracytoplasmic membrane (chromatophores) of the photosynthetic bacterium chromatium vinosum was investigated. trinitrobenzenesulfonate was employed to modify the primary amino groups of phosphatidylethanolamine and proteins. the experimental results indicate that upon the chemical modification of intact cells, the amino groups on the inner surface of the chromatophores are trinitrophenylated, while upon the modificatio ... | 1977 | 591499 |
| on the mechanism of glycolate synthesis by chromatium and chlorella. | | 1978 | 623491 |
| superoxide dismutase from an anaerobic photosynthetic bacterium, chromatium vinosum. | | 1978 | 626504 |
| the effects of uncoupler on the rates of cytochrome oxidation and reduction in the photosynthetic bacterium, chromatium. evidence for a possible cytochrome switching. | the uncoupler, m-chlorocarbonyl cyanide phenylhydrazone (cccp) added to anerobic, dark-adapted whole cells of chromatium vinosum is found to speed the reduction of cytochrome after oxidation by laser or by steady illumination and, subject to unknown factors, to slow the laser-induced oxidation. there is considerable evidence, including spectra and loss of low-temperature oxidizability that this results from a switch of the identity of the cytochrome oxidized from the low-potential cytochrome c-5 ... | 1978 | 629959 |
| [study of the state of intracellular iron in photosynthesizing purple sulfur bacteria using the mossbauer effect]. | the work presents the results of the first stage of the study on the valent and structural state and function of the iron atoms in the donor-acceptor environment of the photosynthetic reaction centres of purple bacteria. 57fe was introduced by cultivating the microorganisms in a medium enriched in this isotope. at 77 k the maxima observed in the mössbauer spectra in intact freeze-dried cells at a speed of +2 mm/s and --1 mm/s are attributed to doublets 1.11 with the isomer ahifts of 1.3 and 0.5 ... | 1978 | 634286 |
| amino acid sequence of a ferredoxin from chlorobium thiosulfatophilum strain tassajara, a photosynthetic green sulfur bacterium. | we have determined the amino acid sequence of a ferredoxin from a photosynthetic green sulfur bacterium, chlorobium thiosulfatophilum strain tassajara. it contains 61 amino acid residues with 9 cysteines, and 8 of the 9 were located at positions corresponding to those in clostridial-type ferredoxins. other structural features were closer to those of ferredoxins from another photosynthetic bacterium, c. limicola, than to those of non-photosynthetic bacteria. compared with ferredoxin from chromati ... | 1978 | 659399 |
| [polarographic study of chlorophyll in aqueous solutions of detergents and in the composition of a chlorophyll-protein complex]. | | 1978 | 667146 |
| pseudomonas ovalis ferredoxin: similarity to azotobacter and chromatium ferredoxins. | | 1978 | 680138 |
| [molecular organization of the long-wave complexes of purple photosynthesizing bacteria. effect of pronase on the b890 complex of chromatium minutissium and rhodopseudomonas palustris]. | the pronase action on the long-wave complexes b890 from two different purple bacteria has been investigated. differences in the kinetics of decrease of the reaction center photochemical activity of electron-donor activity of cytochromes and of destruction of bx890 (875) forms have been discovered. different rates of the proteolysis of rc proteins were revealed by sds-gel-electrophoresis. the heavy protein of rc was the first to degrade. the photochemical transformations deltaa890(875) in b890 co ... | 1978 | 683188 |
| isolation and characterization of the lipopolysaccharide of thiocapsa roseopersicina. | the lipopolysaccharide from thiocapsa roseopersicina was isolated by phenol/water, being found in the water phase. it is cleaved into a polysaccharide moiety (degraded polysaccharide) and lipid a by hydrolysis with 10% acetic acid (100 degree c, 3 h). d-mannose, l-rhamnose, 3-amino-3, 6-dideoxy-d-galactose and d-glucose are the major constituents of the degraded polysaccharide. 2-o-methyl-l-rhamnose, 3-o-methyl-d-mannose, d-galactose, glucosamine and quinovosamine are minor constituents. d-glyce ... | 1978 | 710428 |
| oxygen-stable hydrogenase and assay. | | 1978 | 713840 |
| purification of (4fe-4s)1--2--ferredoxins (high-potential iron--sulfur proteins) from bacteria. | | 1978 | 713842 |