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characterization of the beta-adrenergic receptors in the lower urinary tract of the cat.the corpus-fundus and the outlet region of the urinary bladder of the cat was studied in vitro to characterize the beta-adrenergic receptors. in the corpus-fundus only beta1-receptors were found in contrast to the outlet region where the beta-receptors had both beta1-and beta2-characteristics.197722927
factors affecting the incidence and anti-salmonella activity of the anaerobic caecal flora of the young chick.thirty-two different types of anaerobic bacteria isolated from chickens have been tested for anti-salmonella activity in vitro. under the conditions of the test only bacteroides hypermegas and a bifidobacterium sp. were shown to inhibit the salmonellas and this was attributed to the production of volatile fatty acids (vfa's) coupled with a low ph. when these organisms were tested in newly hatched chicks no inhibition of s. typhimurium occurred. possible explanations for this observation are cons ...197934658
safety of hepatitis-b vaccines containing intact 20 nm particles. 197985121
human breast carcinoma antigen is immunologically related to the polypeptide of the group-specific glycoprotein of mouse mammary tumor virus.we have shown [mesa-tejada, r., keydar, i., ramanarayanan, m., ohno, t., fenoglio, c. & spiegelman, s. (1978) proc. natl. acad. sci. usa 75, 1529--1533] that an antigen immunologically related to gp52, a 52,000-dalton glycoprotein of the mouse mammary tumor virus, can be identified in sections of human breast cancer by means of an indirect immunoperoxidase technique. the specificity of the reaction was established by absorption experiments which revealed that only purified gp52, or material cont ...197988056
prolonged survival of corneal allografts in rabbits treated with cyclosporin a. 197990771
selective vital staining of companion cells of potato tuber and parsnip root with neutral red.when staining the internal phloem region of a potato tuber with the vital stain neutral red, it was observed that files of elongated cells of narrow diameter were heavily stained and were easily distinguishable from the more isodiametric parenchyma cells, many of which did not stain with neutral red. the elongated cells were identified as companion cells by locating the adjacent sieve-tube members through counterstaining with aniline blue and reviewing under violet light. of a number of other pl ...197991243
babesia rodhaini, babesia bovis, and babesia bigemina: analysis and sorting of red cells from infected mouse or calf blood by flow fluorimetry using 33258 hoechst. 197992419
deficiency of the ninth component of complement in man.the studies of serum from a case with c9 (the ninth component of complement) deficiency are described. a 29-year-old woman in good health was found to have low serum complement levels (ch50). c9 of her serum was undetectable by the hemolytic assay and by the immunochemical analysis but all other components were normal. it was demonstrated that low ch50 of her serum was due to the hemolysis of the sensitized sheep erythrocytes (ea) by the complement components from c1 to c8.197995812
[the lice of the gerbils of tajikistan].2606 gerbils of four species from tajikistan were examined. on them there were found over 14 thousand sucking lice of 10 species, four of which are typical to gerbils. hoplopleura merionidis ferris is a mongolian-turkestan species, which in central asia parasitizes meriones meridianus pallas and m. unguiculatus milne-edw. and in middle asia--m. meridianus and m. erythrourus gray, seldom m. tamariscinus pallas. polyplax chinensis ferris, a parasite of m. meridianus, is a mongolian-turkestan speci ...197995816
[the adrenergic innervation of the thoracic lymphatic duct in dogs in postnatal ontogeny].using histochemical fluorescence technique, studies have been made on the adrenergic innervation of the wall of the thoracal lymphatic duct in postnatal development of puppies. adrenergic fibers were found in the adventitial layer of the duct. during postnatal development of the animals, adrenergic innervation of this duct becomes more perfect, reaching the level of adult animals to the age of 2-3 months.197995857
naturally occurring diatomaceous pneumoconiosis in sub-human primates. 197896157
sarcocystis and related organisms in australian wildlife: i. survey findings in mammals. 1978105152
construction and characterization of e. coli promoter-probe plasmid vectors. i. cloning of promoter-containing dna fragments.derivatives of the escherichia coli drug-resistance plasmid pbr316 have been constructed which act as molecular probes for promoter-containing dna restriction fragments from various prokaryotic genomes. the plasmids, designated pbrh1 and pbrh3b, contain a unique ecori restriction site located within the promoter for the tetracycline resistance (tcr) gene. this site was created by the insertion of a chemically synthesized octanucleotide, containing the ecori cleavage sequence, into the hindiii si ...1979118083
analysis of mitotic nondisjunction with aspergillus nidulans.two methods to detect the induction of nondisjunction with a diploid stable strain of a. nidulans are described. the first method gives only qualitative results, while the second method is quantitative and dose-effect curves can be done. some physiological parameters affecting the induction of nondisjunction can also be studied, because either quiescent or germinating conidia can be treated with the drug under test. some agents inducing nondisjunction were also tested for the induction of point ...1979387402
effects of nitroglycerin on cardiac function and regional blood flow distribution in conscious dogs.the effects of intravenous infusion of nitroglycerin (ntg), 8 and 32 microgram/kg.min for 7 min, and of sublingual ntg, 1.2 mg, were examined on direct and continuous measurements of systemic, coronary, and regional hemodynamics, left ventricular (lv) dimensions, pressures, and myocardial contractility in conscious dogs. ntg induced sustained reductions in lv dimensions and transient increases in heart rate and dp/dt, and decreases in mean arterial pressure. initially ntg increased cardiac outpu ...1978415619
leptazol antagonises the post-synaptic actions of gamma-aminobutyric acid [proceedings]. 1979497563
phylogenetic relationships among the agent of bacillary angiomatosis, bartonella bacilliformis, and other alpha-proteobacteria.bacillary angiomatosis (ba) and chronic bartonellosis are bacterial infections of humans which result in an unusual vascular proliferative tissue response. in order to determine their phylogenetic relationships, we have determined greater than 95% of the 16s rrna sequences for these two organisms by amplification directly from infected ba tissue and from a bartonella bacilliformis lyophilized culture. the ba agent and b. bacilliformis are closely related alpha-proteobacteria (98.5%), although th ...19921378524
immunocytochemical identification of rochalimaea henselae in bacillary (epithelioid) angiomatosis, parenchymal bacillary peliosis, and persistent fever with bacteremia.we report the immunocytochemical identification of rochalimaea henselae, a newly recognized fastidious, gram-negative, warthin-starry-positive organism, as the common pathogen in bacillary angiomatosis (ba), bacillary peliosis (bp) of the liver and spleen, and persistent fever with bacteremia in immunocompromised patients. immunogenic proteins of the r. henselae strain isolated from the blood of a febrile immunocompromised patient with bp of the liver were used to produce primary immune serum in ...19921530106
rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis.nine strains of rochalimaea spp. that were isolated from patients over a period of 4.5 years were characterized for their enzyme activities, cellular fatty acid compositions, and dna interrelatedness among rochalimaea spp., bartonella bacilliformis, and afipia felis (cat scratch disease bacillus). all except one isolate, which was rochalimaea quintana, were determined to belong to a newly proposed species, rochalimaea henselae sp. nov. after recovery from clinical material, colonies required 5 t ...19921537892
molecular characterization and proposal of a neotype strain for bartonella bacilliformis.bartonella bacilliformis, the etiologic agent of bartonellosis, was characterized biochemically and by dna hybridization, guanine-plus-cytosine content, genome size, and 16s rrna sequencing. dnas from the two strains in our collection exhibited 97% relatedness in hydroxyapatite reactions done at 55 degrees c (optimal reassociation criterion) and 100% relatedness in reactions done at 70 degrees c (stringent reassociation criterion). there was no evidence of divergence within the related sequences ...19911715879
16s rrna sequences of bartonella bacilliformis and cat scratch disease bacillus reveal phylogenetic relationships with the alpha-2 subgroup of the class proteobacteria.the primary structures of 16s rrnas of bartonella bacilliformis, an isolate of the cat scratch disease (csd) bacillus, and a strain phenotypically similar to the csd bacillus were determined by reverse transcriptase sequencing. these microorganisms were found to be members of the alpha-2 subgroup of the class proteobacteria. the sequence from b. bacilliformis was most closely related to the rrna of rochalimaea quintana (91.7% homology), the etiologic agent of trench fever. the sequence from the ...19911719021
isoprenoid quinones of "afipia" spp.the isoprenoid quinone contents of seven strains of "afipia felis," the type strains of "a. clevelandensis" and "a. broomeae," and reference strains of three unnamed "afipia" genospecies were determined by reverse-phase high-performance liquid chromatography. the quinone profiles of all "afipia" strains were essentially identical, with ubiquinone 10 as the major component. the identity of ubiquinone 10 was confirmed by mass spectrometry.19911757572
proposal of afipia gen. nov., with afipia felis sp. nov. (formerly the cat scratch disease bacillus), afipia clevelandensis sp. nov. (formerly the cleveland clinic foundation strain), afipia broomeae sp. nov., and three unnamed genospecies.on the basis of phenotypic characterization and dna relatedness determinations, the genus afipia gen. nov., which contains six species, is described. the type species is afipia felis sp. nov. (the cat scratch disease bacillus). afipia clevelandensis sp. nov., afipia broomeae sp. nov., and three unnamed not associated with cat-borne disease. all but one strain (afipia genospecies 3) were isolated from human wound and respiratory sources. all afipia species are gram-negative, oxidase-positive, non ...19911774249
isolation of agent associated with cat scratch disease bacillus from pretibial biopsy.we describe the isolation and cultural characteristics of a gram-negative bacillus that is very similar to the presumed etiologic agent of cat scratch disease. the organism was isolated from a tibial lesion of a male patient who had been hospitalized for severe necrotizing pancreatitis. the significance of the isolate in this patient remains uncertain.19911802540
histopathology of bacillary angiomatosis of lymph node.bacillary angiomatosis, a recently characterized pseudoneoplastic vascular proliferation caused by a bacterium identical or related to the cat-scratch disease bacillus, usually presents as cutaneous lesions. we report the histologic findings of this disease involving the lymph nodes of two immunocompromised patients. the lymph nodes showed patchy involvement by coalescent nodules of proliferated blood vessels lined by plump endothelial cells with pale cytoplasm. there were foci exhibiting mild t ...19912035737
association of epstein-barr virus in epithelioid angiomatosis of aids patients.epithelioid angiomatosis, hemangioma-like vascular proliferations recently described in aids patients, has been associated with the cat scratch disease bacillus. other vascular lesions present in aids patients, in particular kaposi's sarcoma, have been associated with cytomegalovirus (cmv). we investigated the possibility of viral association with epithelioid angiomatosis by analyzing two such lesions, as well as unrelated concurrent skin lesions, for the presence of viral genetic information. c ...19902169709
changes in proximal tubular morphology following hypothermic storage in two preservation solutions. a comparative study using canine and porcine kidneys. 19902238074
osteolytic lesions and bacillary angiomatosis in hiv infection: radiologic differentiation from aids-related kaposi sarcoma.bacillary angiomatosis is a newly recognized multisystem bacterial infectious disease seen in the setting of human immunodeficiency virus (hiv) infection. the disease is marked by cutaneous vascular lesions that contain a bacterium similar to the cat scratch disease bacillus. antibiotic therapy leads to the resolution of both cutaneous and systemic manifestations. of 17 hiv-infected patients with cutaneous lesions of bacillary angiomatosis, six (35%) had symptomatic osteolytic bone lesions that ...19902399342
epithelioid angiomatosis and cat scratch disease bacillus. 19882453767
genetic relatedness of the human immunodeficiency viruses type 1 and 2 (hiv-1, hiv-2) and the simian immunodeficiency virus (siv). 19892660679
a molecular strategy for the study of bacterial invasion.bacterial populations are often clonal, and even within a bacterial species, the frequency of gene exchange and recombination is quite low. consequently, mobile genetic elements--plasmids, bacteriophages, and transposons--have been the central factors in the evolution of pathogenic traits. one central feature of pathogenicity, the capacity to enter epithelial cells, is encoded by the bacterial chromosome of yersinia pseudotuberculosis but by plasmid genes in enteroinvasive escherichia coli. a si ...19872825322
epithelioid haemangioma-like vascular proliferation in aids: manifestation of cat scratch disease bacillus infection?papular and nodular skin lesions that clinically resembled kaposi sarcoma, but histologically showed a distinct epithelioid haemangioma-like appearance, were noted in seven patients with the acquired immunodeficiency syndrome. clusters of bacteria that had the structure of gram-negative rods were identified within each of the vascular proliferations by electron microscopy. the bacteria did not stain with the brown-brenn, acid-fast, or other histochemical stains for infectious organisms, but did ...19882896827
cutaneous vascular lesions and disseminated cat-scratch disease in patients with the acquired immunodeficiency syndrome (aids) and aids-related complex.cutaneous lesions develop frequently in patients infected with human immunodeficiency virus (hiv). we describe the clinical features of four patients with the acquired immunodeficiency syndrome (aids) or aids-related complex who developed angiomatous nodules involving skin and bone, 2 of whom were scratched by a cat. some of these lesions were clinically indistinguishable from kaposi sarcoma. when examined with warthin-starry staining and electron microscopy, these nodules were noted to contain ...19883415105
chlamydial infections. 19863536193
measurement and distribution of vasopressin-converting aminopeptidase activity in rat brain.the aminopeptidase activity in the brain which converts vasopressin into centrally active metabolites, was quantitated on basis of the release of 3h-phe from the substate [3h-phe3]vasopressin and separation by hydrophobic interaction chromatography on mini-columns. after subcellular fractionation of whole rat brain homogenates the highest specific activity of the peptidase was recovered in membrane fractions, in particular microsomes and the p3 fraction, and the cytosol. the peptidase activity w ...19873579938
relationship between overfeeding, metritis and ketosis in high yielding dairy cows.the association between overfeeding before calving, metritis and ketosis in seven dairy herds was investigated. the overall rate of ketonuria in 695 adult cows tested routinely between seven and 14 days post partum was 18 per cent and that of post parturient uterine diseases was 56.5 per cent. eighty per cent of all cows with ketonuria concurrently suffered from post parturient uterine diseases. ketonuria was found by a retrospective analysis to be independently associated with both overfeeding ...19854013037
release of histamine from stomach by vagus-stimulating drugs: association with gastric acid secretion. 19654159042
[utilization of feed energy for milk production. 10. energy standards for dairy cows]. 19744433239
serologic responses to bartonella and afipia antigens in patients with cat scratch disease.to assess the serologic response to afipia and bartonella, previously named rochalimaea, in patients with cat scratch disease (csd) and a healthy control group.19957491236
[rochalimaea henselae, afipia felis and cat-scratch disease].several years ago, rochalimaea henselae has emerged as an agent of bacillary angiomatosis, bacillary peliosis and recurrent septicaemia that generally occur in patients infected with human immunodeficiency virus. an aetiologic role in cat scratch disease is also suspected widely on the basis of a serologic survey. its slow growth and its culture requirement explain that this pathogen, a gram-negative bacterium, could not be isolated until 1990. moreover, blood and tissue samples request lysis an ...19947524253
cat-scratch disease in italy: a serological approach.the results of studies on the serologic responses to afipia felis and rochalimaea henselae in suspected patients for cat scratch disease (csd) are illustrated. this preliminary study performed using indirect immunofluorescence assay, proved negative for a. felis and r. henselae in some patients and positive in others; in a few instances the test was positive for both organisms. additional microbiological and serological studies are needed to clarify the exact role of these microorganisms in caus ...19947526131
persistent infection with bartonella (rochalimaea) henselae or afipia felis is unlikely to be a cause of chronic fatigue syndrome. 19947528553
cat-scratch disease in hawaii: etiology and seroepidemiology.to study the etiology and seroepidemiology of cat-scratch disease (csd) in hawaii.19957608806
investigations of culture and properties of afipia spp.the culture conditions of afipia felis, a. broomeae, a. clevelandensis and three unnamed afipia genospecies were investigated on bcy agar supplemented with different substances known as growth factors of legionella spp. and, furthermore, with sodium chloride and other salts. the organisms were found to be susceptible to a certain degree to byproducts of the autoclaving which are scavenged by activated by charcoal. growth was weakly enhanced by ferric pyrophosphate, cystein.hcl, and alpha-ketoglu ...19957734825
[cat-scratch disease and bacillary angiomatosis. an old and a new infectious disease with common etiology?].a review of cat-scratch disease (csd) and bacillary angiomatosis (ba) is presented on the basis of published articles. two newly identified bacteria--rochalimaea henselae and afipia felis--have been isolated from patients with csd. preliminary investigations seem to indicate that a. felis is an uncommon cause of the disease. csd may appear as a local suppurative lymphadenopathy or a systemic infection. ba is caused by rochalimaea species and may appear as cutaneous, mucous or visceral angiomas o ...19947998352
detection of rochalimaea henselae dna in specimens from cat scratch disease patients by pcr.a pcr assay was developed by using degenerate primers that allow amplification of a 414-bp fragment of dna from the rickettsia-like organisms rochalimaea henselae and r. quintana. internal oligonucleotides were used as hybridization probes, permitting rapid differentiation of these two rochalimaea species. dnas from 12 different isolates of r. henselae were amplified with the pcr primers, and the resulting 414-bp pcr product hybridized only with the r. henselae-specific probe. dnas from four dif ...19948027347
rochalimaea henselae organisms possess an elevated capacity of binding to peripheral blood lymphocytes from patients with cat scratch disease.cat scratch disease (csd) is a clinical condition whose aetiological agent, according to recent findings, is of bacterial origin. two gram-negative bacteria are invoked as causative agents of csd, namely afipia felis and rochalimaea henselae. in this paper, five patients with suspected csd were studied in terms of binding capacity of a. felis and r. henselae to their own peripheral blood lymphocytes (pbl). this parameter was correlated with serum antibody titres to both a. felis and r. henselae, ...19948152395
monoclonal antibodies to afipia felis--a putative agent of cat scratch disease.twelve hybridoma clones producing monoclonal antibodies to afipia felis, a putative causative agent of cat scratch disease, were derived from balb/c mice immunized with a felis. all 12 monoclonal antibodies were species-specific for a felis and reacted with lipopolysaccharide antigens of a felis. these monoclonal antibodies belong to immunoglobulin (ig) g1, igg2a, igg2b, igg3, and igm isotypes. all monoclonal antibodies reacted with both agar-grown a felis and tissue culture-propagated a felis. ...19948178767
detection of antibodies to afipia species by the microagglutination test.a microagglutination test with heat-killed, safranin-stained whole-cell antigen for detecting antibodies to afipia species has been developed. an upper limit of the normal titer of antibodies to afipia species was found to be > 10 by testing 430 sera. therefore, a titer of 20 can be considered the lowest titer suggestive of infection. overall, 5-7% of the sera were positive for afipia felis, 3% were positive for afipia broomeae and 11% were positive for afipia clevelandensis. the frequency of se ...19938187793
proteinase k-sensitive and filterable phagosome-lysosome fusion inhibiting factor in afipia felis.afipia felis is one of the putative agents of cat scratch disease (csd). its intracellular location was previously demonstrated in tissue sections. it has been recently grown in human monocytes and in hela cells. we report that a. felis may infect human macrophages and the p 388 d1 murine macrophage cell line, yet is unable to grow in the cell-free culture medium. in both human macrophages and p 388 d1, the organisms multiply in a vacuole after active inhibition of phagosome-lysosome fusion (p-l ...19938271919
granulomatous hepatitis in three children due to cat-scratch disease without peripheral adenopathy. an unrecognized cause of fever of unknown origin.to report the clinical experience of three patients with fever of unknown origin ultimately diagnosed as having cat-scratch granulomatous hepatitis in the absence of peripheral adenopathy.19938362809
antibiotic susceptibilities of afipia felis in axenic medium and in cells.afipia felis, one of the putative agents of cat scratch disease (csd), is a facultative intracellular bacterium. although csd is considered not to be susceptible to antibiotic therapy, sporadic case reports indicated that aminoglycosides may be effective. we determined the in vitro antibiotic susceptibilities of three a. felis strains in axenic medium and in a cell model. in axenic medium, a. felis was susceptible to imipenem, aminoglycosides, and rifampin when using either the broth dilution te ...19938363367
feline zoonotic diseases.many of the feline zoonoses occur more frequently in veterinary personnel owing to their direct contact with cats and the potential for exposure to infected body tissue or fluids. infection of humans with afipia felis, yersinia pestis, francisella tularensis, and other aerobic/anaerobic bacteria may cause great discomfort and in some situations terminal illness. although many systemic fungal agents infect humans and cats, only sporothrix schenckii has been shown to infect humans following direct ...19938421889
cat-scratch disease, bartonella henselae, and the usefulness of routine serological testing for afipia felis. 19958645822
[cat scratch disease. atypical forms].first described by robert debré in 1950, cat scratch disease, usually observed in children and young adults (80% of the case occur in subjects under 18), is the principal cause of chronic benign lymph node enlargement. the centers for disease control at atlanta currently recognize afipia felis and rochalimaea henselae as the causal agents of cat scratch disease. cats transmit the disease to humans by skin scratches or by licking open wounds. the bacilli can also be transmitted by dogs, monkeys, ...19968685112
new pathogens, and diseases old and new. i) afipia felis and rochalimaea. ii) parvovirus b 19. iii) herpesvirus 6.the paper describes events that in the last fifteen years, have led to the identification of the aetiological agents of three widely known diseases: cat scratch disease, erythema infectiosum and exanthem subitum. the particular features of afipia felis and rochalimaea, parvovirus b 19 and herpesvirus 6 are presented. the paternity of new diseases (i.e. bacillary angiomatosis, bacillary peliosis hepatitis, les-like syndrome, chronic fatigue syndrome, petechial glove and sock syndrome, etc.) has a ...19958710408
[visceral localizations of cat-scratch disease in an immunocompetent patient].locoregional expression of cat scratch disease is well known, but despite advances in microbiology over the last 10 years leading to the description of two new bacteria (afipia felis, bartonella henselae) the infective agent responsible for cat scratch syndrome remains unknown. until the 80s, only one systemic disease was attributed to infection with a germ in the bartonella genus: trench fever. with the onset of the aids epidemic, new clinical syndromes caused by bartonella bacteria have been d ...19968729380
an analysis of lymph node dna for possible bacterial agents of cat-scratch disease.recent investigations have implicated afipia felis and rochalimaea henselae as possible agents of cat-scratch disease (csd). we studied lymph nodes with necrotizing granulomas characteristic of csd for a. felis and r. henselae dna so that the relationship of these organisms to lymph nodes with necrotizing granulomas of unknown etiology might be better defined. we examined formalin-fixed, paraffin-embedded lymph node biopsies with necrotizing granulomas suggestive of csd from 28 children obtained ...19958736593
[cat scratch disease in immunocompetent children].first cause of chronic benign lymphadenopathy, cat scratch disease occurs mainly in children and young adults. the nature of its bacterial agents is not definitely established, but atlanta's cdc actually considers afipia felis and rochalimaea hensaela as the agents of the disease. it usually presents as chronic adenitis but there are severe forms of the disease with systemic manifestation. in such forms, new techniques such as serology and molecular biology are particularly useful for the diagno ...19968762962
evaluation of serological response to bartonella henselae, bartonella quintana and afipia felis antigens in 64 patients with suspected cat-scratch disease.the serological response to bartonella henselae, b. quintana, and afipia felis was assessed by an indirect fluorescence antibody test (ifat) in 64 patients with suspected cat-scratch disease (csd) recruited from the bordeaux area in france. blood samples were collected from 57 patients with chronic lymphadenopathy who underwent lymph-node biopsy with suggestive histopathologic features of csd, and from an additional 7 patients with suspected csd who underwent surgical incision and drainage becau ...19968893399
bacillary angiomatosis: microbiology, histopathology, clinical presentation, diagnosis and management.bacillary angiomatosis is known to be caused by a rickettsial organism; rochalimaea henselae. this causative agent has been compared with different microorganisms and clinical conditions that appear in similar settings buy have been clearly differentiated from them; e.i. cat-scratch disease (afipia felis), bartonella bacilliformis, other rochalimaea sp., kaposi's sarcoma, lobular capillary hemangioma, angiosarcoma, and epithelioid hemangioma. clinically the bacillary angiomatosis (ba) skin lesio ...19958703269
[cat scratch disease].cat scratch disease (csd) is usually manifested in children and young adults as a benign, chronic self-limited lymphadenopathy. however, various atypical presentations have been described, especially in immunocompromised hosts. 1 of 5 cases of csd diagnosed here during 1985-1993 is presented. in 3 cases the diagnosis was supported by lymph node biopsy in which the organisms were visualized by warthin-starry silver impregnation staining. recent publications indicate that afipia felis and rochalim ...19947927015
a chemically defined liquid medium that supports primary isolation of rochalimaea (bartonella) henselae from blood and tissue specimens.rochalimaea (bartonella) henselae is a fastidious, slowly growing, gram-negative bacillus that is an etiologic agent of bacillary angiomatosis, cat scratch disease, and related syndromes. accumulation of direct microbiologic evidence of the relationship between the organism and the syndromes compatible with cat scratch disease has been hindered by the difficulties in the primary isolation of the organism from infected tissue specimens. a chemically defined liquid medium was developed to support ...19957538511
retinal and choroidal manifestations of cat-scratch disease.the ability to diagnose cat-scratch disease (csd) has been facilitated greatly by the recent isolation and characterization of bartonella henselae (formerly genus rochalimaea) and afipia felis and by the subsequent development of specific enzyme-linked immunosorbent assay (elisa) serologic tests. this study will help define the patterns of posterior segment ocular involvement in patients with confirmed csd.19989627652
cat scratch disease: the rare role of afipia felis.since its isolation in 1988, afipia felis has been associated with cat scratch disease (csd) in only one report and its role in csd has been questioned. we have cultured a. felis from a lymph node of a patient with csd. 16s rrna gene sequencing, dna relatedness studies, fatty acid analysis, and pcr of the a. felis ferredoxin gene showed that the isolate is identical to the previously reported a. felis isolate. to determine the role of a. felis in csd, pcr of the 16s rrna gene followed by hybridi ...19989705382
killing kinetics of intracellular afipia felis treated with amikacin.afipia felis is a facultative intracellular bacterium which multiplies in macrophages following inhibition of phagosome-lysosome (p-l) fusion. when a. felis-infected cells are incubated for 72 h with various antibiotics, only aminoglycosides are found to be bactericidal. we therefore studied the killing of intracellular a. felis by amikacin, and its relationship with the restoration of p-l fusion. amikacin reduced the number of a. felis from 8.5 x 10(5) to 3.5 x 102 cfu/ml within 94 h. p-l fusio ...199810052910
etiology of cat scratch disease: comparison of polymerase chain reaction detection of bartonella (formerly rochalimaea) and afipia felis dna with serology and skin tests.to determine the role of bartonella (formerly rochalimaea) species and afipia felis in cat scratch disease (csd), two polymerase chain reaction (pcr) hybridization assays were developed to detect dna from these organisms. these assays were applied on 89 pus aspirates from skin test-positive csd patients (group 1) and on 137 pus and lymph node specimens from csd suspects (group 2). bartonella dna was detected in 96% of the samples from group 1 patients and in 60% of group 2 samples; however, a. f ...19957535830
[cat-scratch disease and disease caused by bartonella (rochalimaea)].the aetiology of cat scratch disease remains controversial since both afipia felis and bartonella (rochalimaea) henselae have been isolated from diseased lymph nodes. bartonella henselae, bartonella (rochalimaea) quintana and bartonella (rochalimaea) elizabethae cause endocarditis and bartonella bacilliformis cause septicemia (oroya's fever) in non-immunocompromized patients, and bartonella henselae and bartonella quintana cause fever, bacillary angiomatosis, and visceral peliosis in human immun ...19957534931
antibody response in rabbits infected with rochalimaea henselae, rochalimaea quintana and afipia felis.antibody responses in three pairs of rabbits inoculated with live rochalimaea henselae, rochalimaea quintana and afipia felis were studied by enzyme immunoassay with whole-cell and bacterial sonicates as antigen. no differences in measured antibody responses were found with the two types of antigen preparation. two rabbits did not respond with antibody production. in the remaining rabbits there was a low-titred antibody response that showed no significant cross-reaction with related bacteria. af ...19947534093
immunoelectrophoretic characterization and cross-reactivity of rochalimaea henselae, rochalimaea quintana and afipia felis.the soluble antigens of rochalimaea henselae, rochalimaea quintana and afipia felis were characterized by crossed immunoelectrophoresis using bacterial sonicates as antigens against pooled hyperimmune rabbit sera. a precipitin pattern was drawn for each bacterium and shown to be reproducible and stable even when normal or preimmune rabbit serum was incorporated in the intermediate gel. by this technique 56 antigens were identified from r. henselae, 49 from r. quintana, and 39 from a. felis. the ...19947534092
detection of rochalimaea henselae dna by polymerase chain reaction from suppurative nodes of children with cat-scratch disease.a polymerase chain reaction (pcr) assay was designed to amplify dna from rochalimaea henselae, rochalimaea quintana and afipia felis in the purulent material from lymph nodes in three patients with clinical cat-scratch disease (csd) and two patients with lymphadenitis from other causes. all of the patients with csd had positive immunofluorescent antibody serology for r. henselae, while none of the controls was positive. pcr amplification confirmed the presence of r. henselae dna and the absence ...19947531320
detection of bartonella henselae and afipia felis dna by polymerase chain reaction in specimens from patients with cat scratch disease.polymerase chain reaction (pcr) amplification and colorimetric identification of amplicons were performed to detect bartonella henselae and afipia felis dna in specimens from patients who were clinically and histologically suspected of having cat scratch disease. pcr products were revealed using 2% ethidium bromide agarose-gel electrophoresis and identified with specific probes in a commercial colorimetric hybridization assay (deia) (gen-eti-k; diasorin, italy). six paraffin-embedded lymph node ...200011205638
dual role for afipia felis and rochalimaea henselae in cat-scratch disease. 19957531265
cat-scratch disease: epidemiology, aetiology and treatment.cat-scratch disease (csd) is a clinical syndrome that usually presents as a self-limiting lymphadenopathy associated with a cat scratch or bite. commonly affecting children and young adults, it has a worldwide distribution. in temperate climates, higher rates are reported in the autumn and winter, which can be attributed to the seasonal breeding of the domestic cat. the organism responsible was identified in 1983, having eluded detection for 50 years. initially, afipia felis was thought to be th ...200111440202
legionella-like and other amoebal pathogens as agents of community-acquired pneumonia.we tested serum specimens from three groups of patients with pneumonia by indirect immunofluorescence against legionella-like amoebal pathogens (llaps) 1-7, 9, 10, 12, 13; parachlamydia acanthamoeba strains bn 9 and hall's coccus; and afipia felis. we found that llaps play a role (albeit an infrequent one) in community-acquired pneumonia, usually as a co-pathogen but sometimes as the sole identified pathogen.200111747734
the aquatic budding bacterium blastobacter denitrificans is a nitrogen-fixing symbiont of aeschynomene indica.blastobacter spp. are freshwater bacteria that form rosette structures by cellular attachment to a common base. comparative analyses of ribosomal 16s rrna gene and internally transcribed spacer region sequences indicated that b. denitrificans is a member of the alpha-subdivision of proteobacteria. among the alpha-proteobacteria, b. denitrificans was related to a cluster of genera, including rhodopseudomonas palustris, afipia felis, nitrobacter hamburgensis, and bradyrhizobium spp. although the p ...200211872460
description of afipia birgiae sp. nov. and afipia massiliensis sp. nov. and recognition of afipia felis genospecies a.on the basis of phenotypic characterization and dna relatedness, two novel species are proposed, afipia birgiae sp. nov. (type strain 34632t = cip 106344t = ccug 43108t) and afipia massiliensis sp. nov. (type strain 34633t = cip 107022t = ccug 45153t). a new genospecies is described, named afipia felis genospecies a, closely related to afipia felis. the complexity encountered in the taxonomy of the bradyrhizobiaceae group within the alpha-2 subgroup of the proteobacteria is discussed and the des ...200212361286
rapid polymerase chain reaction-based confirmation of cat scratch disease and bartonella henselae infection.cat scratch disease (csd) commonly occurs secondary to bartonella henselae infection, and the diagnosis has traditionally been made by microscopic findings, the identification of organisms by cytochemistry, and clinical history. however, cytochemical analysis tends to be very difficult to interpret, and histology alone may be insufficient to establish a definitive diagnosis of csd.200312741894
bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester.a strictly aerobic, mesophilic bacterium, strain amx 51(t), was isolated from anaerobic digester sludge. cells were gram-negative, motile, non-sporulating, straight to curved rods with one polar flagellum. the isolate had phenotypic traits of the genus bosea, including cellular fatty acid and substrate utilization profiles. physiological characteristics and antibiotic susceptibility were determined. phylogenetic analysis revealed that strain amx 51(t) was a member of the alpha-proteobacteria, mo ...200313130002
rare bacterium of new genus isolated with prolonged enrichment culture.dynamic change in microbial flora was monitored with an oxygen electrode. the 1st phase microorganisms, which first grew well in lb medium, were followed by the 2nd phase microorganisms, which supposedly assimilated microbial cells of the 1st phase and their metabolites. in a similar way, a change in microbial flora was observed from the 1st phase to the 4th phase in 84 hr. based on this observation, prolonged enrichment culture was done for as long as two months to increase the ratio of existen ...200414745160
removal of odorous sulphur-containing gases by a new isolate from activated sludge.biological treatment of odorous sulphur-containing compounds is attracting attention due to its benign eco-friendliness, energy-savings and low operating costs. as the biological treatment efficiency of dimethylsulphide (dms) reported was often low and variable, selection of useful dms-degrading microorganisms is of importance for the enhancement of the biological deodorizing process. this paper reports the successful isolation of a dms-degrading bacterium from activated sludge, using the enrich ...200415484773
downregulation of human polymorphonuclear cell activities exerted by microorganisms belonging to the alpha-2 subgroup of proteobacteria (afipia felis and rochalimaea henselae).intracellular pathogens have evolved effective mechanisms in order to survive in an intracellular environment, thus avoiding destruction by phagocytic cells. in this regard, a correlation between resistance to phagocytic killing and expression of pathogenic potency has been established. in this report, we have studied the interaction between human polymorphonuclear cells (pmn) and two gram-negative microorganisms, afipia felis and rochalimaea henselae, which belong to the alpha-2 subgroup of the ...19947528238
molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds.this study is the first demonstration that a diverse facultatively methylotrophic microbiota exists in some antarctic locations. pcr amplification of genes diagnostic for methylotrophs was carried out with bacterial dna isolated from 14 soil and sediment samples from ten locations on signy island, south orkney islands, antarctica. genes encoding the mxaf of methanol dehydrogenase, the fdxa for afipia ferredoxin, the msma of methanesulfonate monooxygenase, and the 16s rrna gene of methylobacteriu ...200516104352
phylogenetic analysis of bradyrhizobium japonicum and photosynthetic stem-nodulating bacteria from aeschynomene species grown in separated geographical regions.nearly complete and short partial 16s rrna sequences were derived from pcr-amplified ribosomal dnas of bradyrhizobium japonicum usda 136 and usda 110 and five strains of bacteriochlorophyll-synthesizing bacteria isolated from stem nodules of aeschynomene indica and other aeschynomene species growing in different geographic regions, including india, the philippines and north america. we confirmed that the five stem-nodulating strains examined synthesize bacteriochlorophyll a, and the absorption s ...199416349221
infection-associated vascular lesions in acquired immunodeficiency syndrome patients.several reports have recently appeared in the literature describing "unique" non-neoplastic vascular lesions in patients with the acquired immunodeficiency syndrome (aids). these lesions may be mistaken clinically and histologically for kaposi's sarcoma. the terms epithelioid angiomatosis, epithelioid or histiocytoid hemangioma, and pyogenic granuloma have all been used to describe a similar entity in which cat scratch disease bacillus (csdb) was subsequently identified. lesions closely resembli ...19892551814
effect of synexin on aggregation and fusion of chromaffin granule ghosts at ph 6.fusion of chromaffin granule ghosts was induced by synexin at ph 6, 37 degrees c, in the presence of 10(-7) m ca2+. to study the kinetics and extent of this fusion process we employed two assays that monitored continuously mixing of aqueous contents or membrane mixing by fluorescence intensity increases. in both assays chromaffin granule ghosts were either labeled on the membrane or in the included aqueous phase. the ratios of blank to labeled chromaffin granule ghosts were varied from 1 to 10. ...19872443172
du nouveau sur la maladie des griffes du chat.an update on cat scratch disease: cat scratch disease is an uncommon and infectious disease of man characterized by regional lymphadenopathy following a scratch or a bite from a cat or other inoculation wounds. the role of the cat in the transmission of the disease is uncertain. this paper describes the disease, reviews different hypotheses on the etiology taking into account the recently reported discovery of a cat scratch disease bacillus and summarizes the diagnostic criteria.198617422660
looking for cat scratch disease bacillus. 19852410749
pseudoneoplastic infection of bone in acquired immunodeficiency syndrome. a case report involving the cat-scratch disease bacillus. 19912045405
bacillary angiomatosis: a systemic opportunistic infection with prominent cutaneous manifestations.bacillary angiomatosis is an opportunistic infection with systemic manifestations. although most cases have occurred in human immunodeficiency virus-positive patients, other immunosuppressed patients, and even seemingly immunocompetent individuals, can become infected. in addition to the well-characterized cutaneous manifestations, visceral involvement can occur and may be the only locus of infection. lymphadenopathy, bone or soft-tissue masses, fever, and hepatosplenomegaly can be presenting si ...19911931568
the afipia toolbox and its use to isolate flagellar mutants.afipia felis, a gram-negative alphaproteobacterium, has been implicated as one of the causative agents of cat scratch disease. to identify and begin to examine the virulence traits of this organism, we developed and tested a highly efficient transposon delivery system and a stable plasmid vector expressing green fluorescent protein. the transposome system is based on a tn5-derived transposon and a phage restriction endonuclease type i inhibitor. electroporation of this construct produced a libra ...201019961544
murine antibody responses distinguish rochalimaea henselae from rochalimaea quintana.rochalimaea henselae causes persistent bacteremia, bacillary angiomatosis, and parenchymal bacillary peliosis. detection of a specific antibody response to r. henselae infection may represent an alternative to cultivation as a means of diagnosis. we assessed the specificity of induced murine antibodies for antigens from r. henselae and the closely related species r. quintana. groups of cd-1 mice were inoculated with whole organisms of six strains of r. henselae and two of r. quintana. pre- and p ...19921629326
intracellular growth of afipia felis, a putative etiologic agent of cat scratch disease.the organism afipia felis, which is though to be an etiologic agent of cat scratch disease, is a gram-negative rod that is clearly seen in infected tissue but is very difficult to isolate from clinical specimens; there has been only one report to date of the successful isolation and maintenance of the bacterium on artificial medium. we have found that a. felis will attach, invade via phagocytosis, and multiply intracellularly within the phagosomes of primary human monocytes and hela cells. once ...19921587595
acute meningoencephalitis associated with seroconversion to "afipia felis". 19921354315
comparison of ct and mr in 400 patients with suspected disease of the brain and cervical spinal cord. 20143957605
induced thermal resistance in the mouse ear.the mouse ear (pinna) was used to investigate the effect of two hyperthermic treatments. heating was by immersion in hot water at 43.5 degrees c. a single treatment of about 50 minutes was required to cause necrosis in 50% of the ears heated. when heat treatment was given in two equal fractions the total heating time had to be increased if the interval between fractions was greater than four hours. by 24 hours a total treatment of about 100 minutes was required, indicating almost complete recove ...1979444822
male fertility after successful chemotherapy for lymphoblastic leukaemia. 200091850
[irradiation combined with bleomycin. 5-year results with 27 o.r.l. epitheliomas (proceedings)]. 199985709
x-associated immunocompetence. 199984980
general-practice training, exeter style. 200087562
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