| a chemically defined liquid medium that supports primary isolation of rochalimaea (bartonella) henselae from blood and tissue specimens. | rochalimaea (bartonella) henselae is a fastidious, slowly growing, gram-negative bacillus that is an etiologic agent of bacillary angiomatosis, cat scratch disease, and related syndromes. accumulation of direct microbiologic evidence of the relationship between the organism and the syndromes compatible with cat scratch disease has been hindered by the difficulties in the primary isolation of the organism from infected tissue specimens. a chemically defined liquid medium was developed to support ... | 1995 | 7538511 |
| genetic and phenetic analyses of bradyrhizobium strains nodulating peanut (arachis hypogaea l.) roots. | seventeen bradyrhizobium sp. strains and one azorhizobium strain were compared on the basis of five genetic and phenetic features: (i) partial sequence analyses of the 16s rrna gene (rdna), (ii) randomly amplified dna polymorphisms (rapd) using three oligonucleotide primers, (iii) total cellular protein profiles, (iv) utilization of 21 aliphatic and 22 aromatic substrates, and (v) intrinsic resistances to seven antibiotics. partial 16s rdna analysis revealed the presence of only two rdna homolog ... | 1995 | 7538280 |
| etiology of cat scratch disease: comparison of polymerase chain reaction detection of bartonella (formerly rochalimaea) and afipia felis dna with serology and skin tests. | to determine the role of bartonella (formerly rochalimaea) species and afipia felis in cat scratch disease (csd), two polymerase chain reaction (pcr) hybridization assays were developed to detect dna from these organisms. these assays were applied on 89 pus aspirates from skin test-positive csd patients (group 1) and on 137 pus and lymph node specimens from csd suspects (group 2). bartonella dna was detected in 96% of the samples from group 1 patients and in 60% of group 2 samples; however, a. f ... | 1995 | 7535830 |
| [cat-scratch disease and disease caused by bartonella (rochalimaea)]. | the aetiology of cat scratch disease remains controversial since both afipia felis and bartonella (rochalimaea) henselae have been isolated from diseased lymph nodes. bartonella henselae, bartonella (rochalimaea) quintana and bartonella (rochalimaea) elizabethae cause endocarditis and bartonella bacilliformis cause septicemia (oroya's fever) in non-immunocompromized patients, and bartonella henselae and bartonella quintana cause fever, bacillary angiomatosis, and visceral peliosis in human immun ... | 1995 | 7534931 |
| antibody response in rabbits infected with rochalimaea henselae, rochalimaea quintana and afipia felis. | antibody responses in three pairs of rabbits inoculated with live rochalimaea henselae, rochalimaea quintana and afipia felis were studied by enzyme immunoassay with whole-cell and bacterial sonicates as antigen. no differences in measured antibody responses were found with the two types of antigen preparation. two rabbits did not respond with antibody production. in the remaining rabbits there was a low-titred antibody response that showed no significant cross-reaction with related bacteria. af ... | 1994 | 7534093 |
| immunoelectrophoretic characterization and cross-reactivity of rochalimaea henselae, rochalimaea quintana and afipia felis. | the soluble antigens of rochalimaea henselae, rochalimaea quintana and afipia felis were characterized by crossed immunoelectrophoresis using bacterial sonicates as antigens against pooled hyperimmune rabbit sera. a precipitin pattern was drawn for each bacterium and shown to be reproducible and stable even when normal or preimmune rabbit serum was incorporated in the intermediate gel. by this technique 56 antigens were identified from r. henselae, 49 from r. quintana, and 39 from a. felis. the ... | 1994 | 7534092 |
| detection of rochalimaea henselae dna by polymerase chain reaction from suppurative nodes of children with cat-scratch disease. | a polymerase chain reaction (pcr) assay was designed to amplify dna from rochalimaea henselae, rochalimaea quintana and afipia felis in the purulent material from lymph nodes in three patients with clinical cat-scratch disease (csd) and two patients with lymphadenitis from other causes. all of the patients with csd had positive immunofluorescent antibody serology for r. henselae, while none of the controls was positive. pcr amplification confirmed the presence of r. henselae dna and the absence ... | 1994 | 7531320 |
| dual role for afipia felis and rochalimaea henselae in cat-scratch disease. | | 1995 | 7531265 |
| persistent infection with bartonella (rochalimaea) henselae or afipia felis is unlikely to be a cause of chronic fatigue syndrome. | | 1994 | 7528553 |
| downregulation of human polymorphonuclear cell activities exerted by microorganisms belonging to the alpha-2 subgroup of proteobacteria (afipia felis and rochalimaea henselae). | intracellular pathogens have evolved effective mechanisms in order to survive in an intracellular environment, thus avoiding destruction by phagocytic cells. in this regard, a correlation between resistance to phagocytic killing and expression of pathogenic potency has been established. in this report, we have studied the interaction between human polymorphonuclear cells (pmn) and two gram-negative microorganisms, afipia felis and rochalimaea henselae, which belong to the alpha-2 subgroup of the ... | 1994 | 7528238 |
| cat-scratch disease in italy: a serological approach. | the results of studies on the serologic responses to afipia felis and rochalimaea henselae in suspected patients for cat scratch disease (csd) are illustrated. this preliminary study performed using indirect immunofluorescence assay, proved negative for a. felis and r. henselae in some patients and positive in others; in a few instances the test was positive for both organisms. additional microbiological and serological studies are needed to clarify the exact role of these microorganisms in caus ... | 1994 | 7526131 |
| [rochalimaea henselae, afipia felis and cat-scratch disease]. | several years ago, rochalimaea henselae has emerged as an agent of bacillary angiomatosis, bacillary peliosis and recurrent septicaemia that generally occur in patients infected with human immunodeficiency virus. an aetiologic role in cat scratch disease is also suspected widely on the basis of a serologic survey. its slow growth and its culture requirement explain that this pathogen, a gram-negative bacterium, could not be isolated until 1990. moreover, blood and tissue samples request lysis an ... | 1994 | 7524253 |
| phenotypic and genotypic characterization of bradyrhizobia nodulating the leguminous tree acacia albida. | rhizobial isolates that were obtained from both surface and deep soil samples in the sahelian and sudano-guinean areas of senegal (west africa) under acacia albida trees were compared with representative strains of known rhizobial species and genera. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of proteins was used to determine the taxonomic positions of these organisms and the relationships between isolates obtained from the surface and isolates obtained from deep soil. ... | 1994 | 7520737 |
| serologic responses to bartonella and afipia antigens in patients with cat scratch disease. | to assess the serologic response to afipia and bartonella, previously named rochalimaea, in patients with cat scratch disease (csd) and a healthy control group. | 1995 | 7491236 |
| proposal of afipia gen. nov., with afipia felis sp. nov. (formerly the cat scratch disease bacillus), afipia clevelandensis sp. nov. (formerly the cleveland clinic foundation strain), afipia broomeae sp. nov., and three unnamed genospecies. | on the basis of phenotypic characterization and dna relatedness determinations, the genus afipia gen. nov., which contains six species, is described. the type species is afipia felis sp. nov. (the cat scratch disease bacillus). afipia clevelandensis sp. nov., afipia broomeae sp. nov., and three unnamed not associated with cat-borne disease. all but one strain (afipia genospecies 3) were isolated from human wound and respiratory sources. all afipia species are gram-negative, oxidase-positive, non ... | 1991 | 1774249 |
| isoprenoid quinones of "afipia" spp. | the isoprenoid quinone contents of seven strains of "afipia felis," the type strains of "a. clevelandensis" and "a. broomeae," and reference strains of three unnamed "afipia" genospecies were determined by reverse-phase high-performance liquid chromatography. the quinone profiles of all "afipia" strains were essentially identical, with ubiquinone 10 as the major component. the identity of ubiquinone 10 was confirmed by mass spectrometry. | 1991 | 1757572 |
| murine antibody responses distinguish rochalimaea henselae from rochalimaea quintana. | rochalimaea henselae causes persistent bacteremia, bacillary angiomatosis, and parenchymal bacillary peliosis. detection of a specific antibody response to r. henselae infection may represent an alternative to cultivation as a means of diagnosis. we assessed the specificity of induced murine antibodies for antigens from r. henselae and the closely related species r. quintana. groups of cd-1 mice were inoculated with whole organisms of six strains of r. henselae and two of r. quintana. pre- and p ... | 1992 | 1629326 |
| intracellular growth of afipia felis, a putative etiologic agent of cat scratch disease. | the organism afipia felis, which is though to be an etiologic agent of cat scratch disease, is a gram-negative rod that is clearly seen in infected tissue but is very difficult to isolate from clinical specimens; there has been only one report to date of the successful isolation and maintenance of the bacterium on artificial medium. we have found that a. felis will attach, invade via phagocytosis, and multiply intracellularly within the phagosomes of primary human monocytes and hela cells. once ... | 1992 | 1587595 |
| [cat scratch disease in immunocompetent children]. | first cause of chronic benign lymphadenopathy, cat scratch disease occurs mainly in children and young adults. the nature of its bacterial agents is not definitely established, but atlanta's cdc actually considers afipia felis and rochalimaea hensaela as the agents of the disease. it usually presents as chronic adenitis but there are severe forms of the disease with systemic manifestation. in such forms, new techniques such as serology and molecular biology are particularly useful for the diagno ... | 1996 | 8762962 |
| rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis. | nine strains of rochalimaea spp. that were isolated from patients over a period of 4.5 years were characterized for their enzyme activities, cellular fatty acid compositions, and dna interrelatedness among rochalimaea spp., bartonella bacilliformis, and afipia felis (cat scratch disease bacillus). all except one isolate, which was rochalimaea quintana, were determined to belong to a newly proposed species, rochalimaea henselae sp. nov. after recovery from clinical material, colonies required 5 t ... | 1992 | 1537892 |
| immunocytochemical identification of rochalimaea henselae in bacillary (epithelioid) angiomatosis, parenchymal bacillary peliosis, and persistent fever with bacteremia. | we report the immunocytochemical identification of rochalimaea henselae, a newly recognized fastidious, gram-negative, warthin-starry-positive organism, as the common pathogen in bacillary angiomatosis (ba), bacillary peliosis (bp) of the liver and spleen, and persistent fever with bacteremia in immunocompromised patients. immunogenic proteins of the r. henselae strain isolated from the blood of a febrile immunocompromised patient with bp of the liver were used to produce primary immune serum in ... | 1992 | 1530106 |
| evidence for a close genealogical relationship between afipia (the causal organism of cat scratch disease), bradyrhizobium japonicum and blastobacter denitrificans. | the primary structures of the small subunit rrna of bradyrhizobium japonicum and blastobacter denitrificans were determined by direct sequencing of enzymatically amplified dna. comparative sequence analysis revealed that bradyrhizobium japonicum and blastobacter denitrificans are members of the alpha-2 subgroup of the proteobacteria and show a very close phylogenetic relationship with the genus afipia. | 1992 | 1383084 |
| phylogenetic relationships among the agent of bacillary angiomatosis, bartonella bacilliformis, and other alpha-proteobacteria. | bacillary angiomatosis (ba) and chronic bartonellosis are bacterial infections of humans which result in an unusual vascular proliferative tissue response. in order to determine their phylogenetic relationships, we have determined greater than 95% of the 16s rrna sequences for these two organisms by amplification directly from infected ba tissue and from a bartonella bacilliformis lyophilized culture. the ba agent and b. bacilliformis are closely related alpha-proteobacteria (98.5%), although th ... | 1992 | 1378524 |
| acute meningoencephalitis associated with seroconversion to "afipia felis". | | 1992 | 1354315 |
| [cat scratch disease]. | cat scratch disease (csd) is usually manifested in children and young adults as a benign, chronic self-limited lymphadenopathy. however, various atypical presentations have been described, especially in immunocompromised hosts. 1 of 5 cases of csd diagnosed here during 1985-1993 is presented. in 3 cases the diagnosis was supported by lymph node biopsy in which the organisms were visualized by warthin-starry silver impregnation staining. recent publications indicate that afipia felis and rochalim ... | 1994 | 7927015 |
| investigations of culture and properties of afipia spp. | the culture conditions of afipia felis, a. broomeae, a. clevelandensis and three unnamed afipia genospecies were investigated on bcy agar supplemented with different substances known as growth factors of legionella spp. and, furthermore, with sodium chloride and other salts. the organisms were found to be susceptible to a certain degree to byproducts of the autoclaving which are scavenged by activated by charcoal. growth was weakly enhanced by ferric pyrophosphate, cystein.hcl, and alpha-ketoglu ... | 1995 | 7734825 |
| cat-scratch disease in hawaii: etiology and seroepidemiology. | to study the etiology and seroepidemiology of cat-scratch disease (csd) in hawaii. | 1995 | 7608806 |
| [cat-scratch disease and bacillary angiomatosis. an old and a new infectious disease with common etiology?]. | a review of cat-scratch disease (csd) and bacillary angiomatosis (ba) is presented on the basis of published articles. two newly identified bacteria--rochalimaea henselae and afipia felis--have been isolated from patients with csd. preliminary investigations seem to indicate that a. felis is an uncommon cause of the disease. csd may appear as a local suppurative lymphadenopathy or a systemic infection. ba is caused by rochalimaea species and may appear as cutaneous, mucous or visceral angiomas o ... | 1994 | 7998352 |
| detection of rochalimaea henselae dna in specimens from cat scratch disease patients by pcr. | a pcr assay was developed by using degenerate primers that allow amplification of a 414-bp fragment of dna from the rickettsia-like organisms rochalimaea henselae and r. quintana. internal oligonucleotides were used as hybridization probes, permitting rapid differentiation of these two rochalimaea species. dnas from 12 different isolates of r. henselae were amplified with the pcr primers, and the resulting 414-bp pcr product hybridized only with the r. henselae-specific probe. dnas from four dif ... | 1994 | 8027347 |
| rochalimaea henselae organisms possess an elevated capacity of binding to peripheral blood lymphocytes from patients with cat scratch disease. | cat scratch disease (csd) is a clinical condition whose aetiological agent, according to recent findings, is of bacterial origin. two gram-negative bacteria are invoked as causative agents of csd, namely afipia felis and rochalimaea henselae. in this paper, five patients with suspected csd were studied in terms of binding capacity of a. felis and r. henselae to their own peripheral blood lymphocytes (pbl). this parameter was correlated with serum antibody titres to both a. felis and r. henselae, ... | 1994 | 8152395 |
| retinal and choroidal manifestations of cat-scratch disease. | the ability to diagnose cat-scratch disease (csd) has been facilitated greatly by the recent isolation and characterization of bartonella henselae (formerly genus rochalimaea) and afipia felis and by the subsequent development of specific enzyme-linked immunosorbent assay (elisa) serologic tests. this study will help define the patterns of posterior segment ocular involvement in patients with confirmed csd. | 1998 | 9627652 |
| monoclonal antibodies to afipia felis--a putative agent of cat scratch disease. | twelve hybridoma clones producing monoclonal antibodies to afipia felis, a putative causative agent of cat scratch disease, were derived from balb/c mice immunized with a felis. all 12 monoclonal antibodies were species-specific for a felis and reacted with lipopolysaccharide antigens of a felis. these monoclonal antibodies belong to immunoglobulin (ig) g1, igg2a, igg2b, igg3, and igm isotypes. all monoclonal antibodies reacted with both agar-grown a felis and tissue culture-propagated a felis. ... | 1994 | 8178767 |
| killing kinetics of intracellular afipia felis treated with amikacin. | afipia felis is a facultative intracellular bacterium which multiplies in macrophages following inhibition of phagosome-lysosome (p-l) fusion. when a. felis-infected cells are incubated for 72 h with various antibiotics, only aminoglycosides are found to be bactericidal. we therefore studied the killing of intracellular a. felis by amikacin, and its relationship with the restoration of p-l fusion. amikacin reduced the number of a. felis from 8.5 x 10(5) to 3.5 x 102 cfu/ml within 94 h. p-l fusio ... | 1998 | 10052910 |
| detection of antibodies to afipia species by the microagglutination test. | a microagglutination test with heat-killed, safranin-stained whole-cell antigen for detecting antibodies to afipia species has been developed. an upper limit of the normal titer of antibodies to afipia species was found to be > 10 by testing 430 sera. therefore, a titer of 20 can be considered the lowest titer suggestive of infection. overall, 5-7% of the sera were positive for afipia felis, 3% were positive for afipia broomeae and 11% were positive for afipia clevelandensis. the frequency of se ... | 1993 | 8187793 |
| proteinase k-sensitive and filterable phagosome-lysosome fusion inhibiting factor in afipia felis. | afipia felis is one of the putative agents of cat scratch disease (csd). its intracellular location was previously demonstrated in tissue sections. it has been recently grown in human monocytes and in hela cells. we report that a. felis may infect human macrophages and the p 388 d1 murine macrophage cell line, yet is unable to grow in the cell-free culture medium. in both human macrophages and p 388 d1, the organisms multiply in a vacuole after active inhibition of phagosome-lysosome fusion (p-l ... | 1993 | 8271919 |
| granulomatous hepatitis in three children due to cat-scratch disease without peripheral adenopathy. an unrecognized cause of fever of unknown origin. | to report the clinical experience of three patients with fever of unknown origin ultimately diagnosed as having cat-scratch granulomatous hepatitis in the absence of peripheral adenopathy. | 1993 | 8362809 |
| antibiotic susceptibilities of afipia felis in axenic medium and in cells. | afipia felis, one of the putative agents of cat scratch disease (csd), is a facultative intracellular bacterium. although csd is considered not to be susceptible to antibiotic therapy, sporadic case reports indicated that aminoglycosides may be effective. we determined the in vitro antibiotic susceptibilities of three a. felis strains in axenic medium and in a cell model. in axenic medium, a. felis was susceptible to imipenem, aminoglycosides, and rifampin when using either the broth dilution te ... | 1993 | 8363367 |
| recent advances in diagnosis and treatment of cat scratch disease. | the cause of cat scratch disease (csd), first described in france in 1950 and in the united states in 1951, was unknown until 1983 when the bacterium in lymph nodes was detected using a warthin-starry silver stain. afipia felis has been an infrequent cause of csd since1988, when this gram-negative bacterium was first isolated from 10 patients with csd. in 1992 bartonella organisms were isolated from immunocompetent and immunocompromised patients. an indirect fluorescent antibody test to detect b ... | 2000 | 11095849 |
| feline zoonotic diseases. | many of the feline zoonoses occur more frequently in veterinary personnel owing to their direct contact with cats and the potential for exposure to infected body tissue or fluids. infection of humans with afipia felis, yersinia pestis, francisella tularensis, and other aerobic/anaerobic bacteria may cause great discomfort and in some situations terminal illness. although many systemic fungal agents infect humans and cats, only sporothrix schenckii has been shown to infect humans following direct ... | 1993 | 8421889 |
| syndrome of rochalimaea henselae adenitis suggesting cat scratch disease. | to describe a clinical syndrome of cat scratch disease caused by rochalimaea henselae, including methods for isolation of the organism from tissue and for identification. | 1993 | 8430978 |
| cat-scratch disease, bartonella henselae, and the usefulness of routine serological testing for afipia felis. | | 1995 | 8645822 |
| [cat scratch disease. atypical forms]. | first described by robert debré in 1950, cat scratch disease, usually observed in children and young adults (80% of the case occur in subjects under 18), is the principal cause of chronic benign lymph node enlargement. the centers for disease control at atlanta currently recognize afipia felis and rochalimaea henselae as the causal agents of cat scratch disease. cats transmit the disease to humans by skin scratches or by licking open wounds. the bacilli can also be transmitted by dogs, monkeys, ... | 1996 | 8685112 |
| bacillary angiomatosis: microbiology, histopathology, clinical presentation, diagnosis and management. | bacillary angiomatosis is known to be caused by a rickettsial organism; rochalimaea henselae. this causative agent has been compared with different microorganisms and clinical conditions that appear in similar settings buy have been clearly differentiated from them; e.i. cat-scratch disease (afipia felis), bartonella bacilliformis, other rochalimaea sp., kaposi's sarcoma, lobular capillary hemangioma, angiosarcoma, and epithelioid hemangioma. clinically the bacillary angiomatosis (ba) skin lesio ... | 1995 | 8703269 |
| identity and potential functions of heterotrophic bacterial isolates from a continuous-upflow fixed-bed reactor for denitrification of drinking water with bacterial polyester as source of carbon and electron donor. | a collection of 186 heterotrophic bacteria, isolated directly from a continuous-upflow fixed-bed reactor for the denitrification of drinking water, in which poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (phbv) granules acted as biofilm carrier, carbon source and electron donor, was studied with regard to taxonomic affiliation and degradation and denitrification characteristics. two granule samples were taken from a fully operating reactor for enumeration and isolation of heterotrophic bacteria. o ... | 2001 | 11518336 |
| new pathogens, and diseases old and new. i) afipia felis and rochalimaea. ii) parvovirus b 19. iii) herpesvirus 6. | the paper describes events that in the last fifteen years, have led to the identification of the aetiological agents of three widely known diseases: cat scratch disease, erythema infectiosum and exanthem subitum. the particular features of afipia felis and rochalimaea, parvovirus b 19 and herpesvirus 6 are presented. the paternity of new diseases (i.e. bacillary angiomatosis, bacillary peliosis hepatitis, les-like syndrome, chronic fatigue syndrome, petechial glove and sock syndrome, etc.) has a ... | 1995 | 8710408 |
| legionella-like and other amoebal pathogens as agents of community-acquired pneumonia. | we tested serum specimens from three groups of patients with pneumonia by indirect immunofluorescence against legionella-like amoebal pathogens (llaps) 1-7, 9, 10, 12, 13; parachlamydia acanthamoeba strains bn 9 and hall's coccus; and afipia felis. we found that llaps play a role (albeit an infrequent one) in community-acquired pneumonia, usually as a co-pathogen but sometimes as the sole identified pathogen. | 2001 | 11747734 |
| [visceral localizations of cat-scratch disease in an immunocompetent patient]. | locoregional expression of cat scratch disease is well known, but despite advances in microbiology over the last 10 years leading to the description of two new bacteria (afipia felis, bartonella henselae) the infective agent responsible for cat scratch syndrome remains unknown. until the 80s, only one systemic disease was attributed to infection with a germ in the bartonella genus: trench fever. with the onset of the aids epidemic, new clinical syndromes caused by bartonella bacteria have been d ... | 1996 | 8729380 |
| an analysis of lymph node dna for possible bacterial agents of cat-scratch disease. | recent investigations have implicated afipia felis and rochalimaea henselae as possible agents of cat-scratch disease (csd). we studied lymph nodes with necrotizing granulomas characteristic of csd for a. felis and r. henselae dna so that the relationship of these organisms to lymph nodes with necrotizing granulomas of unknown etiology might be better defined. we examined formalin-fixed, paraffin-embedded lymph node biopsies with necrotizing granulomas suggestive of csd from 28 children obtained ... | 1995 | 8736593 |
| identification of bartonella henselae and b. quintana 16s rdna sequences by branch-, genus- and species-specific amplification. | given the controversy surrounding the aetiology of cat scratch disease and the association of both bartonella henselae and b. quintana with bacillary angiomatosis, a method for the direct detection in clinical samples of 16s rrna from the proteobacteria alpha subgroup was developed. the primary structure of amplified 16s rdna was determined by cloning and sequencing. three sequences were identified: one corresponded exactly to genbank accession number m73229 (b. henselae); the second was related ... | 1996 | 8810946 |
| evaluation of serological response to bartonella henselae, bartonella quintana and afipia felis antigens in 64 patients with suspected cat-scratch disease. | the serological response to bartonella henselae, b. quintana, and afipia felis was assessed by an indirect fluorescence antibody test (ifat) in 64 patients with suspected cat-scratch disease (csd) recruited from the bordeaux area in france. blood samples were collected from 57 patients with chronic lymphadenopathy who underwent lymph-node biopsy with suggestive histopathologic features of csd, and from an additional 7 patients with suspected csd who underwent surgical incision and drainage becau ... | 1996 | 8893399 |
| will the real agent of cat-scratch disease please stand up? | cat-scratch disease has been recognized since 1889 in association with the oculoglandular syndrome of parinaud. the epidemiologic association with cats was first made in 1931 and further substantiated throughout the years, refining the interaction predominantly to kittens. putative infectious agents have included numerous species of bacteria, chlamydiae, and viruses. the cultivation of afipia spp. in the late 1980s appeared to answer the mystery of the identity of the agent. however, even more r ... | 1996 | 8905096 |
| description of afipia birgiae sp. nov. and afipia massiliensis sp. nov. and recognition of afipia felis genospecies a. | on the basis of phenotypic characterization and dna relatedness, two novel species are proposed, afipia birgiae sp. nov. (type strain 34632t = cip 106344t = ccug 43108t) and afipia massiliensis sp. nov. (type strain 34633t = cip 107022t = ccug 45153t). a new genospecies is described, named afipia felis genospecies a, closely related to afipia felis. the complexity encountered in the taxonomy of the bradyrhizobiaceae group within the alpha-2 subgroup of the proteobacteria is discussed and the des ... | 2002 | 12361286 |
| bacillary angiomatosis: microbiology, histopathology, clinical presentation, diagnosis and management. | bacillary angiomatosis is known to be caused by a rickettsial organism; rochalimaea henselae. this causative agent has been compared with different microorganisms and clinical conditions that appear in similar settings but that have been clearly differentiated from them; e.i. cat-scratch disease (afipia felis), bartonella bacilliformis, other rochalimaea sp., kaposi;s sarcoma, lobular capillary hemangioma, angiosarcoma, and epithelioid hemangioma. clinically the bacillary angiomatosis (ba) skin ... | 1996 | 8916440 |
| production and characterization of mouse monoclonal antibodies against afipia felis. | a series of 10 monoclonal antibodies reacting with afipia felis antigens were selected from mice immunized with live organisms of the reference strain atcc 53690. immunoblotting against sds-page-separated a felis sonicate allowed the antibodies to be classified into three groups: 1) 168-4, -6, -7 and -10 reacted with a 53 kda antigen, 2) 168-1, -3 and -9 reacted with both 53 kda and 60 kda antigens, and 3) 168-2, -5 and -9 reacted with other antigens. antibodies of group 1 did not cross-react wi ... | 1997 | 9137514 |
| identification of afipia felis antigens in culture medium: reaction with human sera. | fourteen protein antigens were identified on sds-page of afipia felis culture supernatant. immunoblotting against 10 monoclonal antibodies obtained from mice infected with live a. felis showed that 4 antibodies reacted with a 56 kda band and 3 with both 56 kda and 62 kda bands. compared with a. felis sonicate, the reacting proteins in culture supernatant showed an increase in molecular mass of 2-3 kda, suggesting that they were more glycosylated. purified antigen obtained by affinity chromatogra ... | 1997 | 9137515 |
| [cat-scratch disease: historical, clinical, phylogenetic and taxonomic aspects]. | the cat-scratch disease (csd) is known as a nosological entity since 1950. it was diagnosed by the clinical symptoms, epidemiologic data, and the intracutaneous test of hanger and rose. the aetiologic agent is bartonella (formerly rochalimaea) henselae occurring in thirty to fifty percent of healthy cats. the gramnegative alpha-2-proteobacteria cause the csd but also fever in healthy humans. patients suffering from aids show bacillary angiomatosis, bacillary peliosis hepatis, endocarditis, and s ... | 1997 | 9198973 |
| afipia clevelandensis antibodies and cross-reactivity with brucella spp. and yersinia enterocolitica o:9. | afipia clevelandensis is a recently described gram-negative bacterium whose potential pathogenic role in human disease is under investigation. only one strain, from the pretibial lesion of a patient hospitalized with necrotizing pancreatitis for 5 months, has been isolated. using an indirect immunofluorescence assay to detect anti-a. clevelandensis antibodies, we found a seroprevalence of 1.5% among 30,194 sera routinely submitted for laboratory diagnosis of rickettsial diseases. however, among ... | 1997 | 9384302 |
| immunopurified extracellular bartonella henselae antigen for detecting specific antibodies by enzyme immunoassay. | protein antigens of bartonella henselae bacterial sonicate supernatant and concentrated cell-free culture filtrate were examined by sds-page. the sonicate supernatant gave 38 bands and the culture filtrate at least 21, of which 18 were of bacterial origin. immunoblotting against 13 monoclonal antibodies obtained from mice infected with live b. henselae showed that 10 of these antibodies reacted with a narrow 225 kda band and varying smears of bands ranging from 36 to 240 kda in the sonicate, but ... | 1997 | 9463512 |
| cat scratch disease: the rare role of afipia felis. | since its isolation in 1988, afipia felis has been associated with cat scratch disease (csd) in only one report and its role in csd has been questioned. we have cultured a. felis from a lymph node of a patient with csd. 16s rrna gene sequencing, dna relatedness studies, fatty acid analysis, and pcr of the a. felis ferredoxin gene showed that the isolate is identical to the previously reported a. felis isolate. to determine the role of a. felis in csd, pcr of the 16s rrna gene followed by hybridi ... | 1998 | 9705382 |
| afipia felis in hospital water supply in association with free-living amoebae. | | 1999 | 10218538 |
| seroprevalence of bartonella henselae in cats in germany. | bartonella henselae and b. quintana infections in man are associated with various clinical manifestations including cat-scratch disease, bacillary angiomatosis and bacteraemia. while cats are the natural reservoir for b. henselae, the source of b. quintana is unclear. in this study, the sera of 713 cats from germany were examined for the presence of antibodies against b. henselae, b. quintana or afipia felis by an indirect immunofluorescence assay (ifa). bartonella-specific antibody titres of > ... | 1999 | 10482296 |
| engineering root exudation of lotus toward the production of two novel carbon compounds leads to the selection of distinct microbial populations in the rhizosphere. | the culture of opine-producing transgenic lotus plants induces the increase in the rhizosphere of bacterial communities that are able to utilize these molecules as sole carbon source. we used transgenic lotus plants producing two opines, namely mannopine and nopaline, to characterize the microbial communities directly influenced by the modification of root exudation. we showed that opine-utilizers represent a large community in the rhizosphere of opine-producing transgenic lotus. this community ... | 2004 | 15259274 |
| prevalence of antibodies to bartonella henselae in patients with suspected cat scratch disease (csd) in italy. | cat scratch disease (csd) is a relatively new diagnosed illness with clinical signs of self-limiting regional lymphadenopathy accompanied by symptoms of fever and malaise, to encephalopathy and neuropathy, occurring after a cat scratch or flea bite. bartonella henselae is now accepted as the etiologic agent of csd. from january 1994 to september 1998, 412 patients were evaluated for suspect csd in italy. sera were tested for antibodies to b. henselae by a commercially available indirect immunofl ... | 1999 | 10485354 |
| analysis of bacterial dna in skin and muscle of the tyrolean iceman offers new insight into the mummification process. | about 80 sequences (16s ribosomal rna gene) of bacterial dna in samples of skin and muscle taken directly from the tyrolean iceman (3350-3100 years b.c.) or recovered during the 1992 archaeological expedition at the alpine site were analyzed to obtain clues to the natural mummification process that allowed the corpse of the neolithic shepherd/hunter to be preserved for more than 5,000 years. the investigation was made more complex by the fact that the surface of the mummy had been swabbed with p ... | 2000 | 10640948 |
| isolation of new fastidious alpha proteobacteria and afipia felis from hospital water supplies by direct plating and amoebal co-culture procedures. | as water is a source of nosocomial infections in hospitals, the presence of fastidious gram-negative bacteria in water samples taken in a university hospital was investigated. water samples were inoculated onto agar plates and into amoebal microplates for co-culture. sixty-eight alpha proteobacteria isolates were obtained and characterized using phenotypic methods and 16s rrna gene sequence comparison. the latter approach divided the strains into seven clusters. of these, one corresponded to pre ... | 2000 | 11102690 |
| detection of bartonella henselae and afipia felis dna by polymerase chain reaction in specimens from patients with cat scratch disease. | polymerase chain reaction (pcr) amplification and colorimetric identification of amplicons were performed to detect bartonella henselae and afipia felis dna in specimens from patients who were clinically and histologically suspected of having cat scratch disease. pcr products were revealed using 2% ethidium bromide agarose-gel electrophoresis and identified with specific probes in a commercial colorimetric hybridization assay (deia) (gen-eti-k; diasorin, italy). six paraffin-embedded lymph node ... | 2000 | 11205638 |
| phylogenetic and dna-dna hybridization analyses of bradyrhizobium species. | the 16s rdna sequence of bradyrhizobium liaoningense was determined and analysed together with sequences of other bradyrhizobium species and related taxa. in addition, dna-dna hybridizations were performed between representative strains of the three bradyrhizobium species. bradyrhizobium liaoningense is genotypically highly related to bradyrhizobium japonicum, whereas bradyrhizobium elkanii is more distantly related to these two species. the fact that afipia, agromonas, blastobacter, nitrobacter ... | 2001 | 11211247 |
| phylogenetic analysis of bradyrhizobium japonicum and photosynthetic stem-nodulating bacteria from aeschynomene species grown in separated geographical regions. | nearly complete and short partial 16s rrna sequences were derived from pcr-amplified ribosomal dnas of bradyrhizobium japonicum usda 136 and usda 110 and five strains of bacteriochlorophyll-synthesizing bacteria isolated from stem nodules of aeschynomene indica and other aeschynomene species growing in different geographic regions, including india, the philippines and north america. we confirmed that the five stem-nodulating strains examined synthesize bacteriochlorophyll a, and the absorption s ... | 1994 | 16349221 |
| afipia felis induces uptake by macrophages directly into a nonendocytic compartment. | afipia felis is a gram-negative bacterium that causes some cases of human cat scratch disease. a. felis can survive and multiply in several mammalian cell types, including macrophages, but the precise intracellular compartmentalization of a. felis-containing phagosomes is unknown. here, we demonstrate that, in murine macrophages, most a. felis-containing phagosomes exclude lysosomal tracer loaded into macrophage lysosomes before, as well as endocytic tracer loaded after, establishment of an infe ... | 2001 | 11404461 |
| cat-scratch disease: epidemiology, aetiology and treatment. | cat-scratch disease (csd) is a clinical syndrome that usually presents as a self-limiting lymphadenopathy associated with a cat scratch or bite. commonly affecting children and young adults, it has a worldwide distribution. in temperate climates, higher rates are reported in the autumn and winter, which can be attributed to the seasonal breeding of the domestic cat. the organism responsible was identified in 1983, having eluded detection for 50 years. initially, afipia felis was thought to be th ... | 2001 | 11440202 |
| a method to purify bacteria-containing phagosomes from infected macrophages. | when small particles, such as microorganisms, are taken up by macrophages, they are wrapped with a portion of the host cell plasma membrane and ingested, creating a new organelle, the phagosome. this phagosome matures stepwise as newly formed endosomes do, finally forming a phagolysosome, a process that contributes to killing of ingested microbes and to the presentation of microbial antigens on the surface of the phagocyte. some pathogenic bacteria, however, reprogram the phagocytic cell in such ... | 2000 | 11549946 |
| aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite zootermopsis angusticollis. | to demonstrate the occurrence of cellulolytic bacteria in the termite zootermopsis angusticollis. | 2002 | 11849325 |
| the aquatic budding bacterium blastobacter denitrificans is a nitrogen-fixing symbiont of aeschynomene indica. | blastobacter spp. are freshwater bacteria that form rosette structures by cellular attachment to a common base. comparative analyses of ribosomal 16s rrna gene and internally transcribed spacer region sequences indicated that b. denitrificans is a member of the alpha-subdivision of proteobacteria. among the alpha-proteobacteria, b. denitrificans was related to a cluster of genera, including rhodopseudomonas palustris, afipia felis, nitrobacter hamburgensis, and bradyrhizobium spp. although the p ... | 2002 | 11872460 |
| prevalent bacterial species and novel phylotypes in advanced noma lesions. | the purpose of this study was to determine the bacterial diversity in advanced noma lesions using culture-independent molecular methods. 16s ribosomal dna bacterial genes from dna isolated from advanced noma lesions of four nigerian children were pcr amplified with universally conserved primers and spirochetal selective primers and cloned into escherichia coli. partial 16s rrna sequences of approximately 500 bases from 212 cloned inserts were used initially to determine species identity or close ... | 2002 | 12037085 |
| tfda-like genes in 2,4-dichlorophenoxyacetic acid-degrading bacteria belonging to the bradyrhizobium-agromonas-nitrobacter-afipia cluster in alpha-proteobacteria. | the 2,4-dichlorophenoxyacetate (2,4-d)/alpha-ketoglutarate dioxygenase gene (tfda) homolog designated tfdaalpha was cloned and characterized from 2,4-d-degrading bacterial strain rd5-c2. this japanese upland soil isolate belongs to the bradyrhizobium-agromonas-nitrobacter-afipia cluster in the alpha subdivision of the class proteobacteria on the basis of its 16s ribosomal dna sequence. sequence analysis showed 56 to 60% identity of tfdaalpha to representative tfda genes. a male-tfdaalpha fusion ... | 2002 | 12089027 |
| patients in the intensive care unit are exposed to amoeba-associated pathogens. | to study the role of amoeba-associated alpha proteobacteria as infecting agents in intensive care units (icus). | 2002 | 12186213 |
| [cat scratch disease. an overview for the ent physician]. | background: cat scratch disease (csd) is a relatively common cause of chronic lymphadenopathy in the usa. in the present paper the authors describe recent advances in the understanding of this disorder focusing on etiology, clinical aspects, diagnostic management, and therapy. etiology: rochalimaea henselae and afipia felis, two gram-negative bacteria, have recently been isolated from lymph node tissue of patients suffering from csd. the current literature reveals that rochalimaea henselae seems ... | 1996 | 8924168 |
| polaromonas and hydrogenophaga species are the predominant bacteria cultured from granular activated carbon filters in water treatment. | identification of the predominating cultivable bacteria in granular activated carbon (gac) filters used in a variety of water treatment plants for selecting representative strains to study the role of bacteria in the removal of dissolved organic matter. | 2009 | 19457026 |
| diagnostic value of the indirect immunofluorescence assay in cat scratch disease with bartonella henselae and afipia felis antigens. | serum samples from 35 cat scratch disease (csd) patients, 180 control patients (123 without lymph node enlargement and 57 with lymph node enlargement not evoking csd), and 102 nonpatient subjects (35 with cat contact and 67 without cat contact) were tested by semiquantitative indirect immunofluorescence assay for the presence of antibodies directed to afipia felis (atcc 53690t) or bartonella henselae (atcc 49882t). the csd group had statistically higher antibody titers against b. henselae than t ... | 1996 | 8991636 |
| in most bradyrhizobium groups sequence comparison of 16s-23s rdna internal transcribed spacer regions corroborates dna-dna hybridizations. | in an extension of a previous small-scale test to assess the use of 16s-23s rdna internal transcribed spacer (its) sequences for rapid grouping of bradyrhizobia, we have sequenced the its region of 32 isolates of bradyrhizobium that had previously been studied using aflp and dna-dna hybridizations. we also included representatives of afipia and rhodopseudomonas. our results indicate that its sequences are very diverse among bradyrhizobia. nevertheless, for most of the bradyrhizobia, the grouping ... | 2003 | 12866847 |
| changing concepts in the systematics of bacterial nitrogen-fixing legume symbionts. | as of february 2003, bacteria that form nitrogen-fixing symbiotic associations with legumes have been confirmed in 44 species of 12 genera. phylogenies of these taxa containing legume symbionts based on the comparative analysis of 16s rdna sequences show that they are not clustered in one lineage but are distributed in the classes alphaproteobacteria and betaproteobacteria, and dispersed over the following nine monophyletic groups, being intermingled with other taxa that do not contain legume sy ... | 2003 | 12949698 |
| bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester. | a strictly aerobic, mesophilic bacterium, strain amx 51(t), was isolated from anaerobic digester sludge. cells were gram-negative, motile, non-sporulating, straight to curved rods with one polar flagellum. the isolate had phenotypic traits of the genus bosea, including cellular fatty acid and substrate utilization profiles. physiological characteristics and antibiotic susceptibility were determined. phylogenetic analysis revealed that strain amx 51(t) was a member of the alpha-proteobacteria, mo ... | 2003 | 13130002 |
| temporal change in culturable phenanthrene degraders in response to long-term exposure to phenanthrene in a soil column system. | widespread environmental contamination by polycyclic aromatic hydrocarbons (pah) has led to increased interest in the use of natural attenuation as a clean-up strategy. however, few bioremediation studies have investigated the behaviour of the indigenous pah-degrading community after long-term exposure to a pah. in this study, a column packed with sandy loam soil was exposed to a solution saturated with phenanthrene ( approximately 1.2 mg l-1) for a 6-month period to examine the temporal respons ... | 2003 | 14510842 |
| usefulness of rpob gene sequencing for identification of afipia and bosea species, including a strategy for choosing discriminative partial sequences. | bacteria belonging to the genera afipia and bosea are amoeba-resisting bacteria that have been recently reported to colonize hospital water supplies and are suspected of being responsible for intensive care unit-acquired pneumonia. identification of these bacteria is now based on determination of the 16s ribosomal dna sequence. however, the 16s rrna gene is not polymorphic enough to ensure discrimination of species defined by dna-dna relatedness. the complete rpob sequences of 20 strains were fi ... | 2003 | 14602635 |
| rare bacterium of new genus isolated with prolonged enrichment culture. | dynamic change in microbial flora was monitored with an oxygen electrode. the 1st phase microorganisms, which first grew well in lb medium, were followed by the 2nd phase microorganisms, which supposedly assimilated microbial cells of the 1st phase and their metabolites. in a similar way, a change in microbial flora was observed from the 1st phase to the 4th phase in 84 hr. based on this observation, prolonged enrichment culture was done for as long as two months to increase the ratio of existen ... | 2004 | 14745160 |
| comparison of the in vitro efficacy of telithromycin (hmr 3647) and levofloxacin with 22 antibiotic compounds against bosea and afipia species. | | 2004 | 14998981 |
| bacterial communities constructed in artificial consortia of bacteria and chlorella vulgaris. | we established artificial consortia of bacteria inoculated from soil and a green alga, chlorella vulgaris nies-227. the bacteria and the alga were mixed in a tube or partitioned in a dialysing culture vessel, and the bacterial composition was examined after cultivation. the community of bacteria formed in the consortia included those phylogenetically closely related to the genera phenylobacterium, brevundimonas, phyllobacterium, afipia, sphingomonas, sandaracinobacter, ramlibacter, ralstonia, ce ... | 2010 | 21576850 |
| removal of odorous sulphur-containing gases by a new isolate from activated sludge. | biological treatment of odorous sulphur-containing compounds is attracting attention due to its benign eco-friendliness, energy-savings and low operating costs. as the biological treatment efficiency of dimethylsulphide (dms) reported was often low and variable, selection of useful dms-degrading microorganisms is of importance for the enhancement of the biological deodorizing process. this paper reports the successful isolation of a dms-degrading bacterium from activated sludge, using the enrich ... | 2004 | 15484773 |
| isolation and properties of methanesulfonate-degrading afipia felis from antarctica and comparison with other strains of a. felis. | three novel strains of methylotrophic afipia felis were isolated from several locations on signy island, antarctica, and a fourth from estuary sediment from the river douro, portugal. they were identified as strains of the alpha-2 proteobacterium a. felis by 16s rrna gene sequence analysis. two strains tested were shown to contain the fdxa gene, diagnostic for a. felis. all strains grew with methanesulfonate (and two strains with dimethylsulfone) as sole carbon substrate. growth on methanesulfon ... | 2005 | 15643932 |
| pet scan-positive cat scratch disease in a patient with t cell lymphoblastic lymphoma. | in patients who have history of lymphoma, a positive positron emission tomography (pet) scan is frequently considered as good evidence for relapse and/or persistent disease. thus, lymph node biopsy is not always done to confirm the diagnosis of relapse or refractory lymphoma before a patient is subjected to further chemotherapy. we report a case of patient with history of t cell lymphoblastic lymphoma who presented again with inguinal lymphadenopathy and positive study on positron emission tomog ... | 2005 | 15755513 |
| evidence of pathogenic microbes in the international space station drinking water: reason for concern? | molecular analyses were carried out on four preflight and six postflight international space station (iss)-associated potable water samples at various stages of purification, storage, and transport, to ascertain their associated microbial diversities and overall microbial burdens. following dna extraction, pcr amplification, and molecular cloning procedures, rdna sequences closely related to pathogenic species of acidovorax, afipia, brevundimonas, propionibacterium, serratia, and others were rec ... | 2004 | 15880908 |
| molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds. | this study is the first demonstration that a diverse facultatively methylotrophic microbiota exists in some antarctic locations. pcr amplification of genes diagnostic for methylotrophs was carried out with bacterial dna isolated from 14 soil and sediment samples from ten locations on signy island, south orkney islands, antarctica. genes encoding the mxaf of methanol dehydrogenase, the fdxa for afipia ferredoxin, the msma of methanesulfonate monooxygenase, and the 16s rrna gene of methylobacteriu ... | 2005 | 16104352 |
| [cat-scratch disease as cause of lymphadenitis colli]. | cat-scratch disease as cause of lymphadenitis colli. cat-scratch disease is a frequent cause of lymphadenitis colli. it mainly affects children and adolescents younger than 21 years. since the clinical picture is not characteristic, a history of contact to cats or kittens is highly valuable for diagnosing the disease. major aspects of the disease concerning epidemiology, diagnostic procedures, clinical presentation and therapy are discussed. | 2005 | 16358204 |
| ameba-associated microorganisms and diagnosis of nosocomial pneumonia. | to elucidate the role of ameba-associated microorganisms (aams) as etiologic agents of pneumonia, we screened for legionella spp., parachlamydia acanthamoeba, afipia sp., bosea spp., bradyrhizobium spp., mesorhizobium amorphae, rasbo bacterium, azorhizobium caulinodans, acanthamoeba polyphaga mimivirus, and conventional microorganisms in 210 pneumonia patients in intensive-care units by using culture, polymerase chain reaction, and serologic testing. these resulted in 59 diagnoses in 40 patients ... | 2006 | 16494750 |
| biodiversity of amoebae and amoeba-resisting bacteria in a hospital water network. | free-living amoebae (fla) are ubiquitous organisms that have been isolated from various domestic water systems, such as cooling towers and hospital water networks. in addition to their own pathogenicity, fla can also act as trojan horses and be naturally infected with amoeba-resisting bacteria (arb) that may be involved in human infections, such as pneumonia. we investigated the biodiversity of bacteria and their amoebal hosts in a hospital water network. using amoebal enrichment on nonnutrient ... | 2006 | 16597941 |
| biogenesis of afipia-containing phagosomes in non-professional phagocytes. | afipia felis is a gram-negative alpha-proteobacterium, a rare cause of human cat scratch disease (csd), and likely a pathogen of amoeba. here, we show that various members of the genus afipia attach to and are taken up by various non-professional phagocytic mammalian cells (epithelial cho, endothelial ea.hy926, epithelial hela, epithelial int407 cells, endothelial hmec-1, endothelial huvec, and fibroblast l929 cells). however, only a. felis was able to do this efficiently. invasion depended on a ... | 2007 | 17306590 |
| lipid microdomain-dependent macropinocytosis determines compartmentation of afipia felis. | phagocytic compartments are specialized endocytic organelles and usually mature along the degradative pathway into phagolysosomes. the rare human pathogen afipia felis localizes to a compartment that is different from canonical phagocytic compartments. here, we present evidence that internalization of afipia by macrophages and unusual phagosome development are considerably decreased by attachment of cholera toxin b subunit to macrophage ganglioside gm1 or by extraction or oxidation of plasma mem ... | 2007 | 17319800 |
| idiopathic facial aseptic granuloma: a multicentre prospective study of 30 cases. | idiopathic facial aseptic granuloma (ifag) was recently described in a single-centre retrospective study as a skin condition that occurs specifically in childhood. | 2007 | 17493068 |
| new afipia and bosea strains isolated from various water sources by amoebal co-culture. | it has been suspected that some species belonging to the alphaproteobacteria might cause pneumonia in humans. it is thus of special interest to isolate new members of this phylum, and to further characterize their pathogenicity. the amoebal co-culture method allowed the isolation of various new bacterial species during the last few years, including fastidious alphaproteobacterial species that were isolated from complex environments. in this work, we isolated new bacterial strains from a drinking ... | 2007 | 17706908 |
| characterization of novel linuron-mineralizing bacterial consortia enriched from long-term linuron-treated agricultural soils. | linuron-mineralizing cultures were enriched from two linuron-treated agricultural soils in the presence and absence of a solid support. the cultures contained linuron-degrading bacteria, which coexisted with bacteria degrading either 3,4-dichloroaniline (3,4-dca) or n,o-dimethylhydroxylamine (n,o-dmha), two common metabolites in the linuron degradation pathway. for one soil, the presence of a solid support enriched for linuron-degrading strains phylogenetically related to but different from thos ... | 2007 | 17991021 |