| [experimental study on intraventricular and intrathecal administration of antibiotics (author's transl)]. | | 1979 | 89640 |
| quantitation of particles in the freeze-fractured nuclear membrane after renal ischemia. | changes in the number and sizes of membrane-associated particles have been quantitated in the protoplasmic (p) and exoplasmic (e) fracture faces of the outer membrane of nuclei isolated from the inner cortex following renal ischemia and reflow in the rat. no changes were observed in the inner nuclear membrane. after 20-min ischemia, the number of particles in both fracture faces decreased. with reflow, the total number of particles decreased after both 20- and 60-min ischemia. the partition coef ... | 1978 | 98907 |
| absence of hydrogen peroxide production by or catalase action in rickettsia prowazeki. | glutamic acid oxidation by rickettsia prowazeki is not accompanied by hydrogen peroxide generation, nor is added hydrogen peroxide degraded, as measured by a manometric technique. | 1978 | 101521 |
| chlamydial genital infections: a growing problem. | | 1979 | 478491 |
| fatty acid composition of gliding bacteria: oral isolates of capnocytophaga compared with sporocytophaga. | the extractable and bound lipids and cellular fatty acids of the gram-negative gliding bacteria, capnocytophaga sputigena, c. gingivalis, and c. ochracea were compared to the non-host-related gliding bacterium sporocytophaga myxococcoides. the extractable lipids represented between 17 and 28% of the cell dry weight, whereas only 2 to 4% of the lipids were in the bound fraction. the methyl esters of the cellular fatty acids were mainly ac15:0, which accounted for 69 to 73% of the total extractabl ... | 1979 | 500207 |
| capnocytophaga: new genus of gram-negative gliding bacteria. iii. physiological characterization. | sixty-eight strains of capnophilic fusiform gram-negative rods from the human oral cavity were subjected to extensive physiologic characterization, tested for susceptibility to various antibiotics, and the mol-percent guanine plus cytosine of each isolate determined. the characteristics of the isolates were compared with 10 fresh and 2 stock isolates of fusobacterium nucleatum. the isolates clearly differed from the fusobacterium species on the basis of mol-percent guanine plus cytosine, end pro ... | 1979 | 518236 |
| ultrastructure of gliding bacteria: scanning electron microscopy of capnocytophaga sputigena, capnocytophaga gingivalis, and capnocytophaga ochracea. | when examined by both light and scanning electron microscopy, capnocytophaga gingivalis, c. sputigena, and c. ochracea displayed three distinct growth zones: the original streak, an intermediate zone, and the advancing edge, or halo zone. on trypticase (bbl microbiology systems)-soy-blood agar, the cells translocated by gliding. c. gingivalis and c. sputigena formed large, irregular isolated colonies, while c. ochracea formed a more confluent cell mass. the cells within the streak zone and in mo ... | 1979 | 528052 |
| [effect of x-rays on human adult skin explants]. | | 1975 | 1233920 |
| nucleotide sequences of 16s rrna encoding genes from capnocytophaga ochracea atcc 33596, capnocytophaga sputigena atcc 33612 and capnocytophaga gingivalis atcc 33624. | | 1992 | 1280812 |
| myocardial protection by retrograde cardioplegic perfusion in the presence of acute coronary artery obstruction. an experimental study. | to investigate retrograde delivery of cardioplegic solutions as a means of enhancing myocardial protection in the presence of coronary artery occlusion, a two-part experimental model was devised. in part 1 (in vitro) the possibility of retroperfusing the entire myocardium during acute occlusion of the left anterior descending artery (lad) was assessed. in part 2 (in vivo) acute lad occlusion was performed in dogs, and during 2 hours of aortic cross-clamping crystalline cardioplegic solution was ... | 1992 | 1287835 |
| [pneumococcus-induced septicemia in normal and splenectomized rabbits]. | a new rabbit model to study the consequences of splenectomy in host resistance to induced pneumococcal septicaemia is presented. a simple, fast, non-invasive and complication-free technique of bacterial inoculation into the rabbit airways, using transcutaneous cannulation of the trachea, is also described. sixteen normal and 24 splenectomised animals were used. the optimal dose of pneumonococcus (serotype b, type iii) was found to be 1 x 10(7) organisms. the above dose of bacteria given transtra ... | 1992 | 1479858 |
| [polymorphonuclear leukocyte-mediated effects on human oral keratinocytes by periodontopathic bacterial extracts]. | in order to investigate the polymorphonuclear leukocyte-mediated oral keratinocyte injury, the author established a method to culture keratinocytes (scc9 and gingival keratinocytes) and subjected to the cell detachment assay by polymorphonuclear leukocytes and/or bacterial ultrasonic extracts from actinobacillus actinomycetemcomitans y4, bacteroides forsythus omz 408, fusobacterium nucleatum atcc 10953, capnocytophaga sputigena 4 and eikenella corrodens 1073. the bacterial extracts alone caused ... | 1992 | 1607829 |
| [bacteriological study on juvenile periodontitis]. | the predominant cultivable microflora of 23 pockets in 15 juvenile periodontitis (jp) patients was studied for the first time in china using the current anaerobic methodology. samples were taken with sterile paper points and dispersed on a vortex mixer. then the diluted samples were plated on the non-selective blood agar plates and selective mgb medium which favors the growth of actinobacillus actimycetemcomitans (aa) and incubated in anaerobic chamber for 5 days. from each sample 15 or more iso ... | 1991 | 1676335 |
| use of synthetic oligonucleotide dna probes for the identification of different strains of fusobacterium nucleatum. | the ability of three different oligonucleotide probes to identify different oral strains of fusobacterium nucleatum was tested. probes 119 and 214 were designed on the basis of known amino acid sequences from the n-terminal end of an outer membrane protein of f. nucleatum strain fev1. probe h 2.1, was a randomly cloned chromosomal dna fragment of 2.1 kbp. the specificities of the probes were examined by testing each of them against a panel of five other species of fusobacterium (six strains), or ... | 1991 | 1837056 |
| in vitro killing of actinobacillus actinomycetemcomitans and capnocytophaga spp. by human neutrophil cathepsin g and elastase. | the purpose of this study was to compare the killing of actinobacillus actinomycetemcomitans with that of capnocytophaga spp. by purified cathepsin g and elastase in vitro. both were sensitive to killing by purified cathepsin g, but only the capnocytophaga spp. were killed by elastase. killing by cathepsin g exhibited logarithmic kinetics, was enhanced slightly by alkaline ph, and was enhanced greatly under hypotonic conditions. treatment of cathepsin g with diisopropyl fluorophosphate significa ... | 1991 | 1879926 |
| isolation and purification of bacteriocin from prevotella intermedia (bacteroides intermedius). | thirty-three out of 46 strains of prevotella intermedia (bacteroides intermedius) isolated from gingival deposits of patients with adult periodontitis possessed the ability to inhibit growth of p. intermedia strain atcc 25611, and 6 of the 33 showed strong inhibitory activity. thirteen isolates did not have the ability to inhibit the growth of p. intermedia strain atcc 25611. an inhibition factor from one of the isolates, p. intermedia th14 which had strong inhibition, was purified and character ... | 1991 | 1920011 |
| microbiological and immunological aspects of experimental periodontal disease in rats: a review article. | animal models in which microbiological and immunological aspects of periodontal disease can be studied prospectively seem well warranted. the rat bears much resemblance to man with respect to periodontal anatomy, development and composition of dental plaque, histopathology of periodontal lesions, and basic immunobiology. furthermore, reproducible methods are available for assessment of periodontal disease in rats, and detectable periodontal destruction can be induced in a few weeks in these anim ... | 1991 | 2002433 |
| regulation of the f plasmid tray promoter in escherichia coli by host and plasmid factors. | f plasmid dna transfer (tra) gene expression in escherichia coli is regulated by chromosome- and f-encoded gene products. to study the relationship among these regulatory factors, we constructed low-copy plasmids containing a phi(tray'-'lacz)hyb gene that couples beta-galactosidase and lac permease synthesis to the f plasmid tray promoter. wild-type transformants maintained high levels of beta-galactosidase over a broad range of culture densities. primer extension analysis of tra mrna from f'lac ... | 1991 | 2002497 |
| effects of chlorhexidine on proteolytic and glycosidic enzyme activities of dental plaque bacteria. | chlorhexidine was tested for its ability to inhibit a wide range of glycosidic and proteolytic enzyme activities produced by treponema denticola, porphyromonas gingivalis, bacteroides intermedius, actinobacillus actinomycemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga orchracea, capnocytophaga sp., actinomyces viscosus, streptococcus mitior, streptococcus mutans, streptococcus sobrinus, streptococcus mitis, streptococcus anginosus, streptococcus oralis and strepto ... | 1991 | 2005230 |
| in vitro killing of oral capnocytophaga by granule fractions of human neutrophils is associated with cathepsin g activity. | the capnocytophaga are inhabitants of the hypoxic human gingival crevice that are normally prevented by neutrophils from causing periodontal and systemic infection. to identify potential nonoxidative bactericidal mechanisms against capnocytophaga within human neutrophils, gel filtration chromatography was used to fractionate neutrophil granule extracts. seven granule fractions, designated a through g, were obtained. the capnocytophaga were most sensitive to killing by fraction d. fraction d exhi ... | 1991 | 2022730 |
| prepubertal periodontitis: a review of diagnostic criteria, pathogenesis, and differential diagnosis. | this paper reviews the literature on prepubertal periodontitis (pp) according to the definition and the classification of pp by page. the generalized form of pp (g-pp) defined by page was identified as the oral manifestation of a systemic disease called leukocyte adhesion deficiency (lad) and thus the pathogenesis is known at the molecular level. on the other hand, the localized form of pp (l-pp) is a disease defined by clinical criteria and its pathogenesis is not known at the molecular level. ... | 1990 | 2137170 |
| preferential adsorption of human neutrophil myeloperoxidase isoform iii by oral bacteria. | neutrophil myeloperoxidase (mpo) adsorbs to bacteria as a pre-requisite for killing by the mpo/hydrogen-peroxide/chloride system. three chromatographically distinct isoforms of mpo (mpo i, mpo ii, and mpo iii) have been isolated from human neutrophils. the purpose of this study was to determine whether oral bacteria--including actinobacillus actinomycetemcomitans, capnocytophaga sputigena, haemophilus aphrophilus, and eikenella corrodens--differentially adsorb the three major isoforms of mpo fro ... | 1990 | 2172341 |
| in vitro sensitivity of oral, gram-negative, facultative bacteria to the bactericidal activity of human neutrophil defensins. | neutrophils play a major role in defending the periodontium against infection by oral, gram-negative, facultative bacteria, such as actinobacillus actinomycetemcomitans, eikenella corrodens, and capnocytophaga spp. we examined the sensitivity of these bacteria to a mixture of low-molecular-weight peptides and highly purified individual defensin peptides (hnp-1, hnp-2, and hnp-3) isolated from human neutrophils. whereas the capnocytophaga spp. strains were killed significantly by the mixed human ... | 1990 | 2254020 |
| bactericidal concentrations of chlorhexidine-digluconate, amine fluoride gel and stannous fluoride gel for subgingival bacteria tested in serum at short contact times. | in vitro inhibitory and bactericidal concentrations in serum of chlorhexidine-digluconate, amine fluoride gel, stannous fluoride gel, stannous fluoride, metronidazole and amoxicillin were determined against bacteroides gingivalis, bacteroides intermedius, fusobacterium nucleatum, actinobacillus actinomycetemcomitans and capnocytophaga sputigena. the minimal inhibitory concentration was assessed by the agar dilution technique. the killing curves and minimal bactericidal concentration of the antim ... | 1989 | 2524581 |
| characterization of coagulase-negative staphylococci by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analyses. | coagulase-negative staphylococci are important nosocomial pathogens. at present, no wholly satisfactory typing scheme exists for these organisms. therefore, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting were assessed as characterization methods. a total of 100 type strains and nontyped isolates representing nine species of coagulase-negative staphylococci were analyzed. each species had a reproducible, characteristic whole-cell banding pattern when analy ... | 1989 | 2584373 |
| towards an epidemiology of political violence in the third world. | political violence is distressingly widespread in many parts of the world. this paper reviews the forms and effects of political violence and devotes particular attention to experiences from central america and southern africa. the forms of violence vary from those which are extensive such as civil unrest and war, to those which are intensive, such as assassinations, disappearances and torture. the effects of violence on health may be direct, such as deaths, disabilities, psychological stress an ... | 1989 | 2652321 |
| an evaluation of the multiple-breath nitrogen washout as a pulmonary function test in dairy cattle. | multiple-breath nitrogen washouts (mbnw) were performed with 21 adult dairy cows (seven normal cows tested on two days each, four clinically normal cows tested on six days, and ten cows with respiratory disease tested on one day each). the functional residual capacity (frc), lung clearance index (lci), becklake index (bi), mixing ratio (mr), pulmonary n2 clearance delay (pcd), index of distribution of inspiration (idi) and ventilatory efficiency (eff) were calculated for each mbnw. the lci, mr, ... | 1989 | 2713778 |
| identification of an additional sendai virus non-structural protein encoded by the p/c mrna. | peptide antiserum and monoclonal antibodies have detected a previously unrecognized small non-structural protein in sendai virus-infected cells. this protein, designated x, appears to represent the c-terminal 95 amino acids of the p protein reading frame. the x protein appears to be almost as abundant as the p protein on a molar basis in vivo. no evidence of a precursor-product relationship between the x and p proteins could be found. | 1987 | 2821189 |
| how to find a mutation behind an inherited disease. | the search for the mutations causing human diseases is reaching new gene areas with increasing speed with new cloned genes or polymorphic gene areas, being reported every week. the almost 300 cloned genes and over 1000 rflp-loci of the human genome can be used as tools to approach most human gene defects. once the linkage between the disease and the rflp has been found, chromosomal "jumping" or pulsed field electrophoresis can be used to separate the dna areas in the neighbourhood of the first f ... | 1986 | 2882725 |
| a genetic study of the konda kapu tribe of coastal andhra pradesh, south india. | phenotype and gene frequencies of two blood group and four red cell enzyme systems were examined in a konda kapu tribal sample of coastal andhra pradesh, south india. the gene frequencies for these systems in konda kapus indicate the middle range values for andhra pradesh tribal populations, excepting the ada and rh(d) systems, where extreme range values are found. further, gene flow is indicated between the konda kapus under study and plain kapus, a neighbouring caste population by calculation ... | 1988 | 2975940 |
| regional localization of cd18, the beta-subunit of the cell surface adhesion molecule lfa-1, on human chromosome 21 by in situ hybridization. | the gene coding for the beta-subunit of the cell surface adhesion glycoprotein lfa-1 has been localized to the tip of the long arm of chromosome 21 at 21q22.1-qter. | 1988 | 3073708 |
| [a case of septicemia due to capnocytophaga sputigena]. | | 1987 | 3119744 |
| [primary leiomyosarcoma of the pulmonary artery. apropos of a case]. | a case of leiomyosarcoma of the pulmonary artery in a 64-year old man without previous cardiovascular disease is reported. the clinical picture, which comprised episodes of paroxysmal dyspnoea associated with acute cor pulmonale, suggested pulmonary embolism. radioisotope perfusion study and pulmonary angiography seemed to confirm this diagnosis, but no improvement was obtained with a prolonged thrombolytic treatment. the presence of a median mass at ct led to exploratory thoracotomy and to the ... | 1987 | 3122693 |
| the comparative cytotoxicity of periodontal bacteria. | the direct cytotoxicity of sonic extracts (se) from nine periodontal bacteria for human gingival fibroblasts (hgf) was compared. equivalent dosages (in terms of protein concentration) of se were used to challenge hgf cultures. the cytotoxic potential of each se was assessed by its ability to (1) inhibit hgf proliferation, as measured by direct cell counts; (2) inhibit 3h-thymidine incorporation in hgf cultures; or (3) cause morphological alterations of the cells in challenged cultures. the highe ... | 1988 | 3204481 |
| a redox system for brain targeted estrogen delivery causes chronic body weight decrease in rats. | the effects of 2 redox based carriers for brain directed delivery of estradiol (cds-e2) and ethinyl estradiol (cds-ee) on body weight were examined in rats. a single dose of cds-e2 (3 mg/kg) decreased weight gain in castrate rats for at least 24 days. the dose response of weight gain and lh suppression were compared 12 days and 12 to 25 after cds-e2 and cds-ee, respectively, in ovariectomized (ovx) rats. weight decrease was detected at a lower dose and was significant for longer after drug treat ... | 1988 | 3347139 |
| regulation of haemonchus contortus populations in sheep: mortality of established worms. | | 1988 | 3372130 |
| genomic relationships and species differentiation in the genus capnocytophaga. | ten epidemiologically independent capnocytophaga strains from clinical materials constituted three distinct dna homology groups corresponding to the established species, capnocytophaga ochracea, sputigena, and gingivalis. the three groups exhibited only low, or even insignificant degrees of genomic relatedness mutually, and to a reference culture of cdc group df-2. all of five capnocytophaga ochracea strains considered were involved in topic infections whereas four strains, isolated from blood b ... | 1987 | 3439382 |
| [a nonuniform sequential procedure for diagnosing staphylococcus aureus]. | the possibility of the sharp differentiation of s. aureus in the nonuniform successive statistical identification procedure with the use of signs ranked by their differential information content. the procedure starting from the signs with greater information content permits the identification of s. aureus in 100% of cases. | 1986 | 3515809 |
| a borrelia-specific monoclonal antibody binds to a flagellar epitope. | in immunofluorescence assays monoclonal antibody h9724 recognized eight species of the spirochetal genus borrelia but not representatives of the genera treponema, leptospira, and spirochaeta. we examined the reactivity of h9724 against subcellular components of borrelia hermsii, an agent of relapsing fever, and b. burgdorferi, the cause of lyme disease. h9724 bound to isolated periplasmic flagella of the two borreliae. in western blots the antibody reacted with the predominant protein in flagell ... | 1986 | 3516878 |
| enzymatic and antigenic characterization of immunoglobulin a1 proteases from bacteroides and capnocytophaga spp. | bacteroides and capnocytophaga species have been implicated as periodontal pathogens. some of these species possess immunoglobulin a1 (iga1) proteases that are capable of cleaving the human iga1 molecule in the hinge region, leaving intact fc alpha and fab alpha fragments. the purpose of this study was to characterize this activity. in addition to iga1 protease activity in already known species, iga1 protease activity was a feature of bacteroides buccalis, bacteroides oralis, bacteroides veroral ... | 1987 | 3546133 |
| epidemiologic studies on possible health effects of intake of pyrolyzates of foods, with reference to mortality among japanese seventh-day adventists. | to elucidate the effect of intake of mutagenic and/or carcinogenic pyrolysis products of proteins and amino acids on carcinogenesis in man, we have undertaken two epidemiologic cohort studies: one concerning the possible association of broiled fish consumption with cancer and the other concerning the cancer mortality among japanese seventh-day adventists. the main findings of these studies are described. | 1986 | 3757950 |
| survival analysis of periodontal sites before and after periodontal therapy. | periodontal diseases appear to progress with bursts of destructive activity at individual sites. one effect of treatment might be to diminish the frequency of such bursts. survival analysis was employed to seek such effects on the periodontal sites of 16 individuals with prior evidence of destructive periodontal disease. the subjects were monitored at bi-monthly intervals and actively breaking down sites were detected using attachment level measurements and the tolerance method of analysis. when ... | 1985 | 3860518 |
| clinical and laboratory studies of a family with a high prevalence of juvenile periodontitis. | the form of periodontitis with onset at puberty and affecting predominantly the first molars and incisors is called juvenile periodontitis (jp). the disease has been the object of intense study because from its analysis may come insights into understanding other, more common, forms of periodontitis. we recently had the opportunity to study an unusual family in which both parents developed jp in their teens. we did clinical examinations, measured leukocyte chemotaxis, analyzed the pocket microflo ... | 1985 | 3863910 |
| metastatic nontraumatic clostridium septicum osteomyelitis. | nontraumatic clostridial infections are rare, but need to be diagnosed and treated early or high morbidity and mortality rates result. we believe that this is the first reported case of metastatic nontraumatic clostridium septicum osteomyelitis. early treatment with surgical debridement and parenteral antibiotics without hyperbaric oxygen was used. an associated occult rectal malignancy was discovered and treated. | 1985 | 3980945 |
| pulmonary arterial luminal diameters in relation to the distribution of the lesions associated with canine dirofilariasis. | | 1985 | 4004658 |
| sensitivity of capnocytophaga species to bactericidal properties of human serum. | capnocytophaga is a newly described genus of gram-negative bacteria which can cause serious oral and extraoral infections in the susceptible host. in the present study, sensitivity of capnocytophaga spp. to the bactericidal properties of human serum was investigated. laboratory strains representative of capnocytophaga sputigena, c. ochracea, and c. gingivalis and seven oral isolates of capnocytophaga spp. obtained in primary culture were determined to be sensitive to killing by pooled normal ser ... | 1985 | 4044029 |
| the effect of prompt local cooling on oedema formation in scalded rat paws. | a standardized, reproducible burn model on rat paw was used and the effect of prompt local cooling on the oedema formation was measured using a newly developed non-invasive method. a transient reduction in oedema formation was observed lasting for a longer period of time with decreasing temperature down to 0 degrees c and increasing cooling time up to 120 min. the decrease in oedema formation was followed by an increase towards or above the amount of oedema in untreated scald injury. this post-c ... | 1985 | 4063871 |
| striatal tyrosine hydroxylases with high and low affinity for tyrosine: implications for the multiple-pool concept of catecholamines. | | 1973 | 4148015 |
| the influence of spinal transection on central nystagmus. | | 1964 | 4157949 |
| some pharmacological properties of a synthetic oxytocin analogue [1-n-carbamoyl-hemicystine-2-o-methyltyrosine]-oxytocin (carbamoyl-methyloxytocin), an antagonist to the neurohypophysial hormones. | 1. a synthetic oxytocin analogue, [1-n-carbamoyl-hemicystine-2-o-methyltyrosine]-oxytocin (carbamoyl-methyloxytocin), has been tested as an antagonist to the actions of oxytocin and vasopressin on the uterus, the mammary gland and blood pressure.2. the analogue inhibited the response of the isolated rat uterus to both oxytocin and vasopressin without itself stimulating the uterus to contract. the responses to equipotent doses of oxytocin and vasopressin were inhibited equally. there was little o ... | 1970 | 4321760 |
| some pharmacological properties of a synthetic oxytocin analogue [1-n-carbamoyl-hemicystine-2-o-methyltyrosine]-oxytocin (carbamoyl-methyloxytocin), an antagonist to the neurohypophysial hormones. | 1. a synthetic oxytocin analogue, [1-n-carbamoyl-hemicystine-2-o-methyltyrosine]-oxytocin (carbamoyl-methyloxytocin), has been tested as an antagonist to the actions of oxytocin and vasopressin on the uterus, the mammary gland and blood pressure.2. the analogue inhibited the response of the isolated rat uterus to both oxytocin and vasopressin without itself stimulating the uterus to contract. the responses to equipotent doses of oxytocin and vasopressin were inhibited equally. there was little o ... | 1970 | 4321760 |
| inhibition of hepatic ribonuclease activity by chronic administration of phenobarbitone. | | 1970 | 4394767 |
| amino acid sequence of a peptide containing the active cysteine residue of histidine ammonia-lyase. | 1. oxidized (polymerized) histidine ammonia-lyase from pseudomonas testosteroni was activated with dithiothreitol and the reduced disulphide-linked cysteine residues of the native enzyme were carboxymethylated with iodo[(14)c]acetate. 2. the activity of the carboxymethylated enzyme was similar to that of the polymerized form and approx. 15% of that of the fully reduced form. 3. a tryptic digest of the [(14)c]carboxymethylated enzyme contained only one radioactive peptide. 4. the amino acid seque ... | 1974 | 4422650 |
| suppressor cells in the rabbit appendix. | | 1974 | 4547314 |
| degree of human genetic isolation measured by isonymy and marital distances in two communities in an italian alpine valley. | | 1972 | 4636780 |
| a rapid method for the electrophoresis of chromatin proteins. | | 1973 | 4723239 |
| biological and chemical comparison of butanol- and phenol-water extracted lipopolysaccharide from capnocytophaga sputigena. | | 1983 | 6227738 |
| effect of human leukocyte extracts and gingival exudate on periodontopathic bacteria. | the effect of leukocyte hydrolytic enzymes on periodontopathic bacteria was examined in vitro. a frozen and thawed extract of human peripheral blood leukocytes (le) and human gingival crevicular exudate (ge) were shown to be able to cause the release of 50% of the radioactivity from a leukotoxic strain of actinobacillus actinomycetemcomitans (aa y4), labeled by 14c. a nonleukotoxic strain (aa 653) was shown to be more susceptible to both le and ge, up to 68% of the total radioactivity was solubi ... | 1983 | 6345380 |
| ultrastructural studies of the effect of human leukocyte extracts on periodontopathic bacteria. | human gingival crevicular exudate (ge) and leukocyte extracts (le) were previously shown to be able to release the bulk of radioactivity from a variety of 14c-labeled oral bacteria. the present study demonstrates that the release of radiolabel by these enzymatic agents from two periodontopathic bacteria (actinobacillus actinomycetemcomitans strain y4 and capnocytophaga sputigena strain 4) is accompanied by both cell wall and cytoplasmatic damage. the possible role of bacterial products released ... | 1983 | 6345381 |
| sodium fluoride susceptibilities of suspected periodontopathic bacteria. | utilizing a blood-based medium, we determined the minimum inhibitory concentrations (mic's) to sodium fluoride (naf) of 45 bacterial strains representing 22 oral species. bacterial susceptibility ranged from 128 micrograms/ml to 2048 micrograms/ml. of those organisms tested, actinobacillus actinomycetemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga ochracea, and actinomyces viscosus were susceptible at lower levels of naf. | 1983 | 6574154 |
| [capnocytophaga sputigena in sinusitis]. | the genus capnocytophaga was reported in the literature for the first time in 1979. capnocytophaga are fusiform gram-negative, slowly growing bacteria that require a high pco2. they belong to the normal buccal flora and they are reported as pathogens in infections of the periodontal region with progressive periodontitis and in septicaemia in patients with immunodeficiencies and buccal ulcerations. the present paper is the first to report capnocytophaga sputigena as a pathogen in sinusitis. the p ... | 1983 | 6670527 |
| cellular fatty acids of capnocytophaga species. | the cellular fatty acid composition of 18 clinical isolates and 4 reference strains of capnocytophaga species (capnocytophaga ochracea, capnocytophaga gingivalis, and capnocytophaga sputigena) was determined by gas-liquid chromatography. the fatty acid profiles of the 22 cultures were essentially identical and were characterized by major amounts (60% or greater) of a saturated, iso-branched-chain, 15-carbon acid (13-methyl-tetradecanoate) and the presence of two relatively uncommon saturated, is ... | 1982 | 7153332 |
| [septicemia caused by capnocytophaga sputigena]. | | 1981 | 7290958 |
| biological and chemical characterization of endotoxin from capnocytophaga sputigena. | an endotoxin was isolated from capnocytophaga sputigena strain 4 by a modification of the hot phenol-water method. the extraction procedure yielded a lipopolysaccharide which accounted for approximately 1.5% of the dry weight of the cells. the material was composed of 18.6% lipid (as c(15) fatty acid), 46.5% neutral sugar including 9.6% hexose, 18.3% 6-deoxy sugar, 1.0% 2-keto-3-deoxy sugar, and 4.8% heptose. hexosamine, protein, and phosphorus were found in quantities amounting to 9.0, 2.9, and ... | 1980 | 7358428 |
| effective method for discriminating between oral bacterial and human alkaline phosphatase activity. | alkaline phosphatase (alpase) activity was quantitatively compared in various kinds of oral bacteria. high alpase activity was detected in 3 species of periodontal bacteria, porphyromonas gingivalis, prevotella intermedia and capnocytophaga sputigena. the alpase activity detected in these bacteria was almost completely inhibited in the presence of 1% sodium dodecyl sulfate (sds). by contrast, the activity of mammalian alpase isoenzymes was not inhibited at all even in the presence of 1% sds. the ... | 1994 | 7478753 |
| an approach to efficacy screening of mouthrinses: studies on a group of french products (i). staining and antimicrobial properties in vitro. | there is a large and increasing number of oral hygiene products available to the general public. as such, it is difficult to demonstrate efficacy for all in long-term home-use studies. the aim of this project was to determine whether screening studies could position the activity and efficacy of a number of antiseptic mouthrinses, available in france, by comparison with an established product. this study reports the experiments in vitro. the products under test were, 4 containing chlorhexidine (e ... | 1995 | 7593703 |
| [aortic endocarditis due to capnocytophaga sputigena]. | | 1995 | 7603114 |
| properties of alkaline phosphatase in the gingival crevicular fluid. | the isoenzymic properties of the alkaline phosphatase (alp) of the gingival crevicular fluid (gcf) were investigated and compared with those in other cells, such as human polymorphonuclear leukocytes (pmns), and human periodontal ligament cells (pdls), and with those of three species of periodontopathic bacteria: porphyromonas gingivalis 381 (p. gingivalis), prevotella intermedia atcc25611 (p. intermedia), and capnocytophaga sputigena atcc33123 (c. sputigena). the biochemical properties of the i ... | 1995 | 7796488 |
| bacteremia by multidrug-resistant capnocytophaga sputigena. | a case of bacteremia caused by a multiresistant strain of capnocytophaga sputigena in a patient with hematological malignancy is described. the strain presented with a pattern of marked resistance to beta-lactams, with mics of > 256 mg/liter for ampicillin, ticarcillin, piperacillin, cefazolin, and cefuroxime, 64 mg/liter for cefotaxime, and 32 mg/liter for ceftazidime. in addition, the mic of ciprofloxacin was 16 mg/liter. both of these groups of antimicrobial agents are frequently used in the ... | 1994 | 8027314 |
| bactericidal activities of synthetic human leukocyte cathepsin g-derived antibiotic peptides and congeners against actinobacillus actinomycetemcomitans and capnocytophaga sputigena. | actinobacillus actinomycetemcomitans and capnocytophaga spp. are gram-negative bacteria implicated in the etiology of periodontal disease (particularly in individuals with neutrophil defects) and life-threatening systemic infections. they are resistant to many antibiotics of microbial origin but are sensitive to the nonoxidative microbicidal action of neutrophils. these organisms are susceptible to the microbicidal effect of cathepsin g but are killed by two distinct mechanisms. the purpose of t ... | 1993 | 8109940 |
| capnocytophaga sputigena bacteremia associated with acute leukemia. | during a three-year period, capnocytophaga sputigena bacteremia occurred in three patients with acute leukemia receiving induction therapy on a hematology ward. oral pathology such as periodontitis or severe mucositis was considered to be the most likely source of bacteremia. all three blood culture isolates were identified as that species by deoxyribonucleic acid (dna) homology studies. because of the phenotypical similarity of capnocytophaga species, it is difficult to differentiate them by co ... | 1993 | 8360517 |
| [subgingival distribution of periodontopathic bacteria in periodontic patients and susceptibility of these bacteria to minocycline-hcl]. | the present study was carried out to examine the distribution of six periodontopathic bacteria in deep periodontal pockets and to reconfirm the effect of periocline on these periodontopathic bacteria. samples from sixty-two periodontal pockets were collected at pocket depths of over 4 mm in twenty-one periodontitis patients aged 43 to 75 years. after sampling, periocline was applied topically to the selected pockets once a week for four weeks and reexamined. the detected rates of the periodontop ... | 1995 | 8583161 |
| degradation of lactoferrin by periodontitis-associated bacteria. | the degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. the degradation of lactoferrin was more extensive by porphyromonas gingivalis and capnocytophaga sputigena, slow by capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia, and very slow or absent by prevotella nigrescens, campylobacter rectus ... | 1996 | 8961558 |
| novel fnr homologues identified in four representative oral facultative anaerobes: capnocytophaga ochracea, capnocytophaga sputigena, haemophilus aphrophilus, and actinobacillus actinomycetemcomitans. | based upon dna sequence data and positive immunochemical reactivity of expressed protein, novel homologues of the fnr family were identified in four representative oral facultative anaerobes: capnocytophaga ochracea, capnocytophaga sputigena, haemophilus aphrophilus, and actinobacillus actinomycetemcomitans. the similarity to e. coli fnr and to hlyx (itself 71% similar to e. coli fnr, while regulating expression of hemolysin operon in actinobacillus pleuropneumoniae) was estimated from the deduc ... | 1996 | 8998988 |
| myeloperoxidase isoform activities released by human neutrophils in response to dental and periodontal bacteria. | myeloperoxidase is an enzyme released by polymorphonuclear leukocytes which has been used, experimentally, as an indicator of periodontal disease activity when measured in gingival crevicular fluid. there are three myeloperoxidase isoforms: mpo i, mpo ii and mpo iii. we examined the activities of myeloperoxidase isoforms released by neutrophils in response to serum-opsonized actinobacillus actinomycetemcomitans, haemophilus aphrophilus, eikenella corrodens, capnocytophaga sputigena and streptoco ... | 1997 | 9151641 |
| hla-d types and serum igg responses to capnocytophaga in diabetes and periodontitis. | serum igg responses to the cell envelope proteins (ceps) from capnocytophaga sputigena, capnocytophaga ochracea, and capnocytophaga gingivalis were examined in periodontally healthy and periodontitis subjects, both with and without type 1 diabetes (n = 60). serum igg responses to ceps were determined by immunoblotting with biotin-goat anti-human igg and an alkaline phosphatase-streptavidin system. reactivity was analyzed by transmission densitometry, digitization, and computer manipulation. the ... | 1997 | 9390475 |
| characterization of protease activities in capnocytophaga spp., porphyromonas gingivalis, prevotella spp., treponema denticola and actinobacillus actinomycetemcomitans. | protease activities in cell sonicates of defined bacterial strains were examined using peptide substrates and class-specific inhibitors. capnocytophaga spp. all produced serine dipeptidyl peptidase activity and arginine/lysine, elastase- and chymotrypsin-like enzymes with some metalloprotease characteristics. the elastase-like activity was strongest in capnocytophaga sputigena, but the others were greatest in capnocytophaga gingivalis. the latter also had a separate arginine-specific enzyme whic ... | 1997 | 9467394 |
| subgingival distribution of periodontopathic bacteria in adult periodontitis and their susceptibility to minocycline-hcl. | the purpose of this study was to investigate the distribution of several periodontopathic bacteria in adult periodontitis, their in vitro susceptibility to minocycline-hcl, and whether the efficacy of the drug changes with a decrease in bacterial susceptibility. twenty-one patients (43 to 75 years old) with 62 periodontal lesions from pockets > or =4 mm participated in the study. after subgingival sampling, an ointment containing 2% minocycline-hcl was applied locally to the selected pockets onc ... | 1998 | 9527568 |
| subgingival microflora associated with nifedipine-induced gingival overgrowth. | the purpose of this study was to examine the composition of subgingival plaque of 140 periodontal lesions in 35 patients with cardiovascular disorders who were administered nifedipine and manifested nifedipine-induced gingival overgrowth (go). age was inversely associated with the go. plaque index and bleeding index showed a significant association with go, while nifedipine dosage and duration of nifedipine therapy were not found to be significant predictors of go. the gingival inflammation as e ... | 1998 | 9660335 |
| subgingival microflora and treatment in prepubertal periodontitis associated with chronic idiopathic neutropenia. | prepubertal periodontitis affects both primary and permanent dentition. the purpose of this study was to examine the composition of subgingival microflora of the permanent dentition in an 11-year-old caucasian female, who had premature exfoliation of her deciduous teeth on her 5th year of age, and the response of this condition to the antibiotic therapy and supportive periodontal care. gingival tissues were highly inflamed and alveolar bone loss was detected radiographically. the girl had experi ... | 1998 | 9763332 |
| detection of dextranase-producing gram-negative oral bacteria. | thirty-one strains of 23 gram-negative oral bacterial species were examined for dextran-degrading activity on agar plates containing blue dextran. one strain each of capnocytophaga ochracea, capnocytophaga sputigena, prevotella loescheii, prevotella melaninogenica and prevotella oralis had detectable dextranase activity. the culture supernatants of p. melaninogenica and p. oralis cells contained dextranases of multiple sizes, but those of the other three species had a single size of enzyme. a 56 ... | 1998 | 9872116 |
| second-trimester abortion caused by capnocytophaga sputigena: case report. | intra-amniotic infection is often the cause of a second-trimester abortion. the bacterial species involved include bacteria with low pathogenicity like ureaplasma urealyticum and various mycoplasma species. in this case we describe an intra-amniotic infection caused by capnocytophaga sputigena, often found in the normal bacterial flora of the oral cavity, but not in the vagina. oral sex during pregnancy was the most probable source of the infection. the aborted fetus showed signs of pneumonia up ... | 1999 | 10458530 |
| microbiology of subgingival plaque from children with localized prepubertal periodontitis. | localized prepubertal periodontitis has been described as a host-defect mediated form of bacterially induced periodontitis, with an early onset and rapid progression around a few teeth in children prior to puberty. to further our understanding of the etiology of this disease, we have examined the microbiological components of subgingival dental plaque in 9 children with localized prepubertal periodontitis to determine if patterns of putative pathogens existed, and have compared these results wit ... | 1987 | 10870471 |
| characterization and cross-reactivity of rabbit antisera to plaque toxins. | the effects of heat labile, high molecular weight water-soluble toxins from bacterial plaque on hl60 promyelocytic cells were examined. on gel filtration, four inhibitors of hl60 cell growth and two inhibitors of hela cell growth (pt1, pt2) were detected. the first and third hl60 cell inhibitors corresponded to the two hela cell inhibitors. the last eluted hl60 cell inhibitor (plaque leukotoxin, pl) did not inhibit hela cell growth. anti-pt2 antibodies reduced the activity of enriched pt2 by 20- ... | 1987 | 10870474 |
| early microbial colonization of permucosal implants in edentulous patients. | in edentulous patients, the microbial colonization of permucosal implants of sintered hydroxyapatite was studied. samples were taken from mucosa and dentures before insertion of implants and from supra- and subgingival sites two to 10 weeks after insertion. in total, five patients and 10 implants with clinically healthy peri-implant tissues were studied. the samples were investigated by dark-field microscopy and anaerobic culture. the supragingival plaque of the implants was dominated by gram-po ... | 1987 | 10872401 |
| microbial composition of supra- and subgingival plaque in subjects with adult periodontitis. | the purpose of the present study was to compare and relate the microbial composition of supra and subgingival plaque in 23 adult periodontitis subjects (mean age 51 +/- 14 years). | 2000 | 11034118 |
| pcr detection of capnocytophaga species in dental plaque samples from children aged 2 to 12 years. | the purpose of this study was to detect the presence of capnocytophaga sputigena, c. ochracea, and c. gingivalis in plaque samples from the toothbrushes of 122 children, using a polymerase chain reaction (pcr) method. the subjects were 25, 85, and 12 children with healthy gingiva, gingivitis, and periodontitis, respectively, ranging in age from 2-12 years old. plaque samples were collected from all erupted tooth sites using a sterile toothbrush. the mean amount of dna recovered from the samples ... | 2001 | 11270602 |
| relationship of periodontopathic bacteria with early-onset periodontitis in down's syndrome. | down's syndrome (ds) patients often develop severe early-onset marginal periodontitis in early adulthood; however, there is little information available on the microbiology of ds periodontitis. | 2001 | 11327065 |
| [utility of prolonged incubation and terminal subcultures of blood cultures from immunocompromised patients]. | the value of blind terminal subcultures (7 and 30 days) and prolonged incubation (30 days) of blood cultures from immunosuppressed patients was analyzed in the fundación favaloro, the fundación para la lucha contra las enfermedades neurológicas de la infancia and the hospital de niños ricardo gutiérrez. a total of 2707 blood cultures and 369 patients were included (transplantation of solid organs 154, oncohematologic disorders 106 and solid tumors 109). bact-alert bottles were incubated at 35 de ... | 2001 | 11594009 |
| periodontopathic bacterial infection in childhood. | little information is available on periodontopathic bacterial infection in childhood. we assessed the prevalence by age of 10 putative periodontopathic microorganisms in periodontally healthy children using a polymerase chain reaction (pcr) assay. | 2002 | 11846195 |
| [in vitro sensitivity of oral gram-negative bacteria to the bactericidal activity of defensins]. | oral gram-negative bacteria, including actinobacillus actinomycetemcomitans, porphyromonas gingivalis, capnocytophaga sputigena, provotella intermedius, provotella melaninogenicus, have been associated with destructive periodontal infection. an ultrasensitive antibacterial assay was applied to determine the sensitivity of a battery of oral gram-negative bacteria to the bactericidal effects of the isolated human neutrophil defensins (hnp) and two kinds of rabbit defensins np1 and np2. all species ... | 1998 | 12078176 |
| rate of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. | although microbiological studies have identified more than 400 bacterial species in periodontal pockets, only a limited number have been implicated as periodontal pathogens. the purpose of this study was to investigate the incidence of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. bacterial samples were collected with sterile paper points from the deepest periodontal pockets ((5 mm) of 203 patients: 92 males and 111 females, aged 35-55 years. the samples were cul ... | 2004 | 15508748 |
| molecular identification of an invasive gingival bacterial community. | a woman with neutropenia developed gingival hyperplasia. biopsy showed invasion of gingival tissue with mats of filamentous organisms, and molecular analysis by polymerase chain reaction and fluorescence in situ hybridization revealed capnocytophaga sputigena, leptotrichia species, and fusobacterium nucleatum. oral bacterial flora may cause invasive gingival disease with hyperplasia in immunocompromised patients. | 2005 | 15937752 |
| chromosome- and plasmid-encoded beta-lactamases in capnocytophaga spp. | chromosome- and plasmid-encoded cfxa2 and cfxa3 beta-lactamases were detected in capnocytophaga spp. from oral sources in france, norway, and the united states. unidentified chromosome-encoded beta-lactamases were present in capnocytophaga sputigena. nucleotide sequence analysis of the cfxa3-encoding plasmid from c. ochracea revealed an unreported insertion sequence (iscoc1) upstream of the cfxa gene. | 2005 | 16127077 |
| distribution of 10 periodontal bacteria in saliva samples from japanese children and their mothers. | we analyzed the distribution of 10 periodontal bacteria species (porphyromonas gingivalis, tannerella forsythensis, prevotella intermedia, prevotella nigrescens, campylobacter rectus, eikenella corrodens, actinobacillus actinomycetemcomitans, capnocytophaga ochracea, capnocytophaga sputigena, and treponema denticola) in children, and then compared their distribution in those children and their mothers, with special attention given to three of the species known as the red complex (p. gingivalis, ... | 2006 | 16269128 |
| infection at titanium implants with or without a clinical diagnosis of inflammation. | to assess the microbiota at implants diagnosed with peri-implantitis, implant mucositis, or being clinically healthy. | 2007 | 17517058 |
| clinical and microbiological analysis of subjects treated with brånemark or astratech implants: a 7-year follow-up study. | to assess the impact of different implant systems on the clinical conditions and the microbiota at implants, and whether the presence of bacteria at tooth sites was predictive of the presence at implant sites. | 2008 | 18261121 |
| microbiological evaluation of primary endodontic infections in teeth with and without sinus tract. | to examine the microbiological status of primary endodontic infections in teeth with and without a sinus tract. | 2008 | 18422583 |
| first case of bacteremia due to chromosome-encoded cfxa3-beta-lactamase-producing capnocytophaga sputigena in a pediatric patient with acute erythroblastic leukemia. | bacteremia due to capnocytophaga sputigena occurred in a 4-year and 9-month-old japanese girl patient with acute erythroblastic leukemia in shinshu university hospital, japan. on her admission to the hospital, she had a temperature of 38.2 degrees c with canker sore. prior to the commencement of chemotherapy, peripheral blood culture was carried out with the bact/alert 3d system ver. 4.00d (biomerieux japan ltd., tokyo, japan) using both the pf and the sn bottles. at 48 hrs of incubation, the sy ... | 2008 | 18499560 |
| [capnocytophaga sputigena pleuropneumonitis: a case report]. | community-acquired pneumonia is complicated by purulent pleural effusion in approximately 28% of affected children. its incidence has increased over the last decade. streptococcus pneumoniae is the most frequent causal microorganism, encountered in roughly 80% of cases. we report the case of an immunocompetent 13-year-old teenager wearing orthodontic braces who suffered from pleuropneumonia due to capnocytophaga sputigena. to date, this gram-negative bacillus, a commensal of the buccal cavity, h ... | 2008 | 18804973 |