| solid phase indirect radioimmunoassays for the rapid diagnosis of sindbis virus antigen. | indirect radioimmunoassays have been developed for the rapid detection of sindbis virus. dilutions of sindbis virus from tissue culture fluids have been immobilized and allowed to react with rabbit anti-sindbis virus antibodies. the bound antibodies were assayed either by 125i-labelled anti-rabbit igg-antibodies or alternatively by addition of human complement and 125i-labelled anti-human c1q antibodies or 125i-labelled protein a. | 1978 | 84855 |
| analysis of anti-sperm sera for t/t locus-specific antibody. | an objective antiglobulin radioimmunoassay for the analysis of anti-spermatozoa antisera has been used to characterize more than 30 alloantisera. each of these antisera was raised against spermatozoa from mice carrying t/t regin mutations. whilst all of the antisera had considerable activity for mouse spermatozoa, none of them was found to contain antibodies specific for t/t region gene products. a detailed analysis of a single serum revealed that its activity is for antigens which are non-polym ... | 1979 | 95255 |
| capnocytophaga: new genus of gram-negative gliding bacteria. iii. physiological characterization. | sixty-eight strains of capnophilic fusiform gram-negative rods from the human oral cavity were subjected to extensive physiologic characterization, tested for susceptibility to various antibiotics, and the mol-percent guanine plus cytosine of each isolate determined. the characteristics of the isolates were compared with 10 fresh and 2 stock isolates of fusobacterium nucleatum. the isolates clearly differed from the fusobacterium species on the basis of mol-percent guanine plus cytosine, end pro ... | 1979 | 518236 |
| ultrastructure of gliding bacteria: scanning electron microscopy of capnocytophaga sputigena, capnocytophaga gingivalis, and capnocytophaga ochracea. | when examined by both light and scanning electron microscopy, capnocytophaga gingivalis, c. sputigena, and c. ochracea displayed three distinct growth zones: the original streak, an intermediate zone, and the advancing edge, or halo zone. on trypticase (bbl microbiology systems)-soy-blood agar, the cells translocated by gliding. c. gingivalis and c. sputigena formed large, irregular isolated colonies, while c. ochracea formed a more confluent cell mass. the cells within the streak zone and in mo ... | 1979 | 528052 |
| effect of repeated bloodletting on the incidence of 2,7-faa-induced rat leukemia. | the oral administration of n,n'-2,7-fuorenylenebisacetamide (2,7-faa) induced leukemia, especially mature granulocytic leukemia, in rats. 2,7-faa was administered to the rats of the wistar strain and a small amount of bloodletting was repeated for a long period. the incidence of leukemia and mature granulocytic leukemia became elevated by bloodletting. the induction of mature granulocytic leukemia might be increased by the promotion of granulopoiesis which had been suppressed by 2,7-faa. | 1976 | 1068628 |
| [effect of x-rays on human adult skin explants]. | | 1975 | 1233920 |
| nucleotide sequences of 16s rrna encoding genes from capnocytophaga ochracea atcc 33596, capnocytophaga sputigena atcc 33612 and capnocytophaga gingivalis atcc 33624. | | 1992 | 1280812 |
| myocardial protection by retrograde cardioplegic perfusion in the presence of acute coronary artery obstruction. an experimental study. | to investigate retrograde delivery of cardioplegic solutions as a means of enhancing myocardial protection in the presence of coronary artery occlusion, a two-part experimental model was devised. in part 1 (in vitro) the possibility of retroperfusing the entire myocardium during acute occlusion of the left anterior descending artery (lad) was assessed. in part 2 (in vivo) acute lad occlusion was performed in dogs, and during 2 hours of aortic cross-clamping crystalline cardioplegic solution was ... | 1992 | 1287835 |
| isolation and characterization of enzymes hydrolyzing chymotrypsin synthetic substrate (enzyme i) and trypsin synthetic substrate (enzyme ii) from the envelope of capnocytophaga gingivalis. | enzymes hydrolyzing chymotrypsin synthetic substrate and trypsin synthetic substrate, referred to as enzyme i and enzyme ii, respectively, were found in the envelope fraction of capnocytophaga gingivalis (atcc 33624). detergent extraction of both enzymes were purified by gel filtration, ion exchange chromatography, and affinity chromatography. the enzyme i was a serine-containing metallo enzyme with a molecular mass of 77 kda. the molecular mass of the enzyme ii was 83 kda, and it was inhibited ... | 1992 | 1406457 |
| hiv education and health education in the united states: a national survey of local school district policies and practices. | to determine the extent to which hiv education and health education policies and practices are required by school districts in the united states, a national probability sample of public school districts was surveyed by mail in 1990. of 2,150 districts selected, 78.1% responded. hiv education was required by 66.9% of districts. of these, the percentage requiring hiv education increased by grade level from 29.7% in kindergarten to 82.3% in 7th grade, then declined to 37.3% by 12th grade. districts ... | 1992 | 1479838 |
| [bacteriological study on juvenile periodontitis]. | the predominant cultivable microflora of 23 pockets in 15 juvenile periodontitis (jp) patients was studied for the first time in china using the current anaerobic methodology. samples were taken with sterile paper points and dispersed on a vortex mixer. then the diluted samples were plated on the non-selective blood agar plates and selective mgb medium which favors the growth of actinobacillus actimycetemcomitans (aa) and incubated in anaerobic chamber for 5 days. from each sample 15 or more iso ... | 1991 | 1676335 |
| bacteriological study of juvenile periodontitis in china. | the predominant cultivable bacteria associated with juvenile periodontitis (jp) in china were studied for the first time. subgingival plaque samples were taken on paper points from 23 diseased sites in 15 jp patients and from 7 healthy sites in 7 control subjects. serially diluted plaque samples were plated on nonselective blood agar and on mgb agar, a selective medium for the isolation of actinobacillus actinomycetemcomitans. fifteen or more isolated colonies from each sample (in sequence witho ... | 1991 | 1832453 |
| the topographical and age distributions of neuroendocrine cells in the normal human appendix. | in order to clarify the histogenesis of appendiceal carcinoid tumours, epithelial (enc) and subepithelial (snc) neuroendocrine cells were counted at four sites in 50 normal appendices stained by standard argyrophil and argentaffin techniques. in general, enc were present in similar number at all sites within the appendix, whereas snc were more numerous at the tip than at the base. the number of enc was similar throughout life, apart from an increase in one neonate and some elderly patients, wher ... | 1991 | 1890548 |
| effects of chlorhexidine on proteolytic and glycosidic enzyme activities of dental plaque bacteria. | chlorhexidine was tested for its ability to inhibit a wide range of glycosidic and proteolytic enzyme activities produced by treponema denticola, porphyromonas gingivalis, bacteroides intermedius, actinobacillus actinomycemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga orchracea, capnocytophaga sp., actinomyces viscosus, streptococcus mitior, streptococcus mutans, streptococcus sobrinus, streptococcus mitis, streptococcus anginosus, streptococcus oralis and strepto ... | 1991 | 2005230 |
| in vitro sensitivity of oral, gram-negative, facultative bacteria to the bactericidal activity of human neutrophil defensins. | neutrophils play a major role in defending the periodontium against infection by oral, gram-negative, facultative bacteria, such as actinobacillus actinomycetemcomitans, eikenella corrodens, and capnocytophaga spp. we examined the sensitivity of these bacteria to a mixture of low-molecular-weight peptides and highly purified individual defensin peptides (hnp-1, hnp-2, and hnp-3) isolated from human neutrophils. whereas the capnocytophaga spp. strains were killed significantly by the mixed human ... | 1990 | 2254020 |
| the presence of bacteria in the oral epithelium in periodontal disease. ii. immunohistochemical identification of bacteria. | serial histological sections of gingiva obtained from each of six advanced adult periodontitis, two localized juvenile periodontitis and two periodontally healthy patients were used for specific identification of bacteria within the oral epithelium and adjacent connective tissue. healthy gingival biopsies served as controls. sections from patients and control biopsies were gram-stained and also screened with antibacterial sera associated with the peroxidase immunocytochemical technique for speci ... | 1986 | 2427680 |
| use of adhesin-specific monoclonal antibodies to identify and localize an adhesin on the surface of capnocytophaga gingivalis dr2001. | monoclonal antibodies capable of inhibiting coaggregation between capnocytophaga gingivalis dr2001 and actinomyces israelii pk16 were used to identify the adhesin on c. gingivalis that mediates the interaction. the monoclonal antibodies were used to demonstrate that a 140-kilodalton polypeptide found in the outer membrane of c. gingivalis was the adhesin responsible for coaggregation. a coaggregation-defective mutant that was unable to coaggregate with a. israelii lacked this large polypeptide. ... | 1989 | 2572552 |
| fetal cortical transplants reduce the thalamic atrophy induced by frontal cortical lesions in newborn rats. | fetal neocortical tissue was grafted into frontal cortex lesion cavities made in newborn rats. after survival periods extending up to 14 months, volumetric measurements of the total thalamus and of the lateral, medial and anterior thalamic compartments showed an amelioration of the thalamic atrophy that normally is found after cortical lesions. these results correspond to previous findings demonstrating interconnections between fetal cortical transplants and the host thalamus. | 1989 | 2710397 |
| specific petit mal anticonvulsants reduce calcium currents in thalamic neurons. | low-threshold calcium current (ltcc) in thalamic neurons is important in generation of normal thalamocortical rhythms, and may be involved in the genesis of abnormal activities such as spike-wave discharges that characterize petit mal epilepsy. ethosuximide and dimethadione, anticonvulsants effective in petit mal, reduced the ltcc when applied to thalamic neurons at clinically relevant concentrations. therapeutic concentrations of phenytoin and carbamazepine, drugs ineffective in the control of ... | 1989 | 2710401 |
| a comparison of the effects of morphine on 5-ht metabolism in the periaqueductal gray, ventromedial medulla and medullary dorsal horn: in vivo electrochemical studies in freely moving rats. | the effect of systemic morphine on serotonin (5-ht) metabolism within the dorsal raphe nucleus (drn) has been investigated by in vivo 5-hydroxyindole electrochemical (peak '3') detection in freely moving rats. morphine caused a weak and delayed, but naloxone-reversible, increase in peak '3'. this increase was poorly, if at all, correlated with the morphine-induced analgesia. finally, stress and/or noxious stimulation had no effect on this signal. these results are compared with our previous stud ... | 1989 | 2776031 |
| an immunohistochemical evaluation of c-erbb-2 expression in human breast carcinoma. | the c-erbb-2 gene codes for a putative transmembrane protein, similar in structure to the epidermal growth factor (egf) receptor. amplification of the gene has been described in a variety of human adenocarcinomas and is particularly well documented in breast carcinoma. it has been suggested that amplification is indicative of poor prognosis and, as such, is comparable with lymph node status as a predictor of clinical outcome. this study examines the suggestion indirectly by an immunohistochemica ... | 1988 | 2849974 |
| the challenge of malaria vaccine: trials and tribulations. | | 1987 | 2880172 |
| how to find a mutation behind an inherited disease. | the search for the mutations causing human diseases is reaching new gene areas with increasing speed with new cloned genes or polymorphic gene areas, being reported every week. the almost 300 cloned genes and over 1000 rflp-loci of the human genome can be used as tools to approach most human gene defects. once the linkage between the disease and the rflp has been found, chromosomal "jumping" or pulsed field electrophoresis can be used to separate the dna areas in the neighbourhood of the first f ... | 1986 | 2882725 |
| a genetic study of the konda kapu tribe of coastal andhra pradesh, south india. | phenotype and gene frequencies of two blood group and four red cell enzyme systems were examined in a konda kapu tribal sample of coastal andhra pradesh, south india. the gene frequencies for these systems in konda kapus indicate the middle range values for andhra pradesh tribal populations, excepting the ada and rh(d) systems, where extreme range values are found. further, gene flow is indicated between the konda kapus under study and plain kapus, a neighbouring caste population by calculation ... | 1988 | 2975940 |
| antimicrobial properties of hydrogen peroxide and sodium bicarbonate individually and in combination against selected oral, gram-negative, facultative bacteria. | the topical application of hydrogen peroxide (h2o2) and sodium bicarbonate (nahco3), individually and in combination, has been used empirically in the treatment of periodontal diseases. in this study, we examined both minimum inhibitory concentrations (mic) and minimum bactericidal concentrations (mbc) of these disinfectants individually and in combination against selected facultative, gram-negative oral bacteria in a microtiter dilution assay. the bacteria studied included actinobacillus actino ... | 1986 | 3016051 |
| a computer-aided analysis of the effect of peripheral nerve transection on tmpase activity of substantia gelatinosa rolandi. | | 1986 | 3020818 |
| ro 15-1788 suppresses the development of kindling through the benzodiazepine receptor. | beta-carboline (norharman) has been shown to produce kindled seizures when given systemically for long periods of time. the expression of the kindled seizure activity can be blocked by ligands of the benzodiazepine receptor suggesting the receptor as a site of vulnerability in the kindling mechanism. the present data show that ro 15-1788, a receptor antagonist, suppresses the development of kindled seizures as demonstrated by the delayed appearance of each behavioral stage and the decreased seve ... | 1986 | 3026559 |
| regional localization of cd18, the beta-subunit of the cell surface adhesion molecule lfa-1, on human chromosome 21 by in situ hybridization. | the gene coding for the beta-subunit of the cell surface adhesion glycoprotein lfa-1 has been localized to the tip of the long arm of chromosome 21 at 21q22.1-qter. | 1988 | 3073708 |
| regulation of net bicarbonate transport in rabbit cortical collecting tubule by peritubular ph, carbon dioxide tension, and bicarbonate concentration. | the effects of changes in peritubular ph, carbon dioxide tension (pco2), and hco3- concentration on net hco3- transport was examined in in vitro perfused cortical collecting tubules (ccts) from unpretreated new zealand white rabbits. lowering peritubular hco3- concentration and ph by reciprocal replacement of hco3- with cl-, significantly stimulated net hco3- absorption. lowering peritubular hco3- concentration and ph, by substitution of hco3- with gluconate, while keeping cl- concentration cons ... | 1986 | 3084564 |
| concanavalin a-stimulated rat spleen cells produce a soluble factor that supports growth of the mouse il-3-dependent cell line ea3.123, but not three other il-3-dependent lines. | several cloned, non-leukaemic mouse haemopoietic cell lines are believed to be uniquely dependent on interleukin-3 (il-3) for their survival. all of these lines require the addition of wehi-3b cell-conditioned medium for their growth. we have examined the growth requirements of four il-3-dependent cell lines and found that only one, ea3.123, is able to grow in medium conditioned by concanavalin a-stimulated rat spleen cells (rat conacm), whereas the other three, ac2, 32d cl-23 and fdc-p2, are no ... | 1986 | 3089920 |
| regulation of haemonchus contortus populations in sheep: mortality of established worms. | | 1988 | 3372130 |
| genomic relationships and species differentiation in the genus capnocytophaga. | ten epidemiologically independent capnocytophaga strains from clinical materials constituted three distinct dna homology groups corresponding to the established species, capnocytophaga ochracea, sputigena, and gingivalis. the three groups exhibited only low, or even insignificant degrees of genomic relatedness mutually, and to a reference culture of cdc group df-2. all of five capnocytophaga ochracea strains considered were involved in topic infections whereas four strains, isolated from blood b ... | 1987 | 3439382 |
| laboratory studies of a family manifesting premature exfoliation of deciduous teeth. | in a previous publication, we described the clinical and radiographic findings of a family in which the children manifested premature exfoliation of the deciduous teeth. we now report for the same family the results of extensive laboratory studies performed on blood and urine, analysis of periodontal microflora, and a family pedigree. we demonstrated the presence of putative periodontal pathogens in the subgingival microflora, elevated levels of serum antibodies reacting to bacteroides gingivali ... | 1986 | 3463573 |
| calcium antagonists and adenine nucleotide metabolism in rat heart. | attempts to identify mechanisms by which calcium antagonists might influence intracellular metabolism have not yet yielded conclusive findings. in this study bepridil, verapamil, nifedipine, and nisoldipine were found to have no influence on the rate of rat heart myosin adenosine triphosphatase or the calcium dependence of myofibrillar adenosine triphosphatase. none of these calcium antagonists alters the rate of reaction of any of the adenine nucleotide catabolic or adenosine salvage enzymes, a ... | 1986 | 3491685 |
| major histocompatibility complex-unrestricted cytolytic activity of human t cells. analysis of precursor frequency and effector phenotype. | the frequency and phenotype of human t cells that mediate major histocompatibility complex (mhc)-unrestricted cytolysis were analyzed. t cell clones were generated by culturing adherent cell-depleted peripheral blood mononuclear cells at a density of 0.3 cell/well with phytohemagglutinin, recombinant interleukin 2 (ril-2), and irradiated autologous peripheral blood mononuclear cells and/or epstein-barr virus-transformed lymphoblastoid cell lines. these conditions were shown to expand a mean of 9 ... | 1987 | 3500232 |
| enzymatic and antigenic characterization of immunoglobulin a1 proteases from bacteroides and capnocytophaga spp. | bacteroides and capnocytophaga species have been implicated as periodontal pathogens. some of these species possess immunoglobulin a1 (iga1) proteases that are capable of cleaving the human iga1 molecule in the hinge region, leaving intact fc alpha and fab alpha fragments. the purpose of this study was to characterize this activity. in addition to iga1 protease activity in already known species, iga1 protease activity was a feature of bacteroides buccalis, bacteroides oralis, bacteroides veroral ... | 1987 | 3546133 |
| [the effect of verbenone on prostaglandin production in vivo]. | | 1986 | 3593548 |
| structural specificity of diamines covalently linked to peptidoglycan for cell growth of veillonella alcalescens and selenomonas ruminantium. | putrescine and cadaverine are essential constituents of the peptidoglycan of veillonella alcalescens, veillonella parvula, and selenomonas ruminantium and are necessary for the growth of these organisms (y. kamio and k. nakamura, j. bacteriol. 169:2881-2884, 1987, and y. kamio, h. pösö, y. terawaki, and l. paulin, j. biol. chem. 261:6585-6589, 1986). in this study, the structural specificity of the diamine requirement for normal cell growth of these bacteria was examined by using a series of dia ... | 1987 | 3654585 |
| hemagglutinating activity of lipopolysaccharides from subgingival plaque bacteria. | lipopolysaccharide (lps) preparations from the bacteroides species b. endodontalis, b. intermedius, b. denticola, and b. melaninogenicus and from fusobacterium nucleatum, haemophilus actinomycetemcomitans, capnocytophaga gingivalis, and eikenella corrodens directly agglutinated erythrocytes of some kinds of animals. lpss from the bacteroides species b. gingivalis, b. asaccharolyticus, b. corporis, and b. loescheii and from capnocytophaga ochracea did not possess any hemagglutinating activity. pr ... | 1987 | 3679548 |
| mathematical model of the metabolism of 123i-16-iodo-9-hexadecenoic acid in an isolated rat heart. validation by comparison with experimental measurements. | the aim of the present study was to demonstrate that it is possible to estimate the intracellular metabolism of a fatty acid labelled with iodine using external radioactivity measurements. 123i-16-iodo-9-hexadecenoic acid (iha) was injected close to the coronary arteries of isolated rat hearts perfused according to the langendorff technique. the time course of the cardiac radioactivity was measured using an ina crystal coupled to an analyser. the obtained curves were analysed using a four-compar ... | 1986 | 3709563 |
| epidemiologic studies on possible health effects of intake of pyrolyzates of foods, with reference to mortality among japanese seventh-day adventists. | to elucidate the effect of intake of mutagenic and/or carcinogenic pyrolysis products of proteins and amino acids on carcinogenesis in man, we have undertaken two epidemiologic cohort studies: one concerning the possible association of broiled fish consumption with cancer and the other concerning the cancer mortality among japanese seventh-day adventists. the main findings of these studies are described. | 1986 | 3757950 |
| gram negative species associated with active destructive periodontal lesions. | apical subgingival plaque samples were taken from 19 subjects exhibiting active destructive periodontal disease. the predominant cultivable gram negative species from 50 active sites were compared to 69 inactive sites of comparable pocket depth and attachment level loss. active disease sites were chosen which showed a significant loss of attachment within a two-month interval. proportions of gram negative rods were higher in active periodontal disease sites than in inactive sites. species which ... | 1985 | 3863838 |
| coaggregation of oral bacteroides species with other bacteria: central role in coaggregation bridges and competitions. | seventy-three freshly isolated oral strains representing 10 bacteroides spp. were tested for their ability to coaggregate with other oral gram-negative and gram-positive bacteria. none coaggregated with any of the gram-negative strains tested, which included capnocytophaga gingivalis, c. ochracea, c. sputigena, and actinobacillus actinomycetemcomitans. strains of bacteroides buccae, b. melaninogenicus, b. oralis, and b. gingivalis failed to coaggregate with any of the gram-positive strains teste ... | 1985 | 3888842 |
| [aids to wound healing. from empirical tradition to a scientific approach]. | | 1985 | 3915246 |
| identification and preliminary characterization of a lectinlike protein from capnocytophaga gingivalis (emended). | a polypeptide believed to be the monomeric form of the lectin responsible for the coaggregation of capnocytophaga gingivalis (emended) and actinomyces israelii has been identified. denaturing polyacrylamide gel electrophoresis and immunoblot analyses were used to distinguish the protein from other proteins in the outer membrane of c. gingivalis. the subunit of the putative lectin has a pi of 8.6 and a molecular weight of 155,000. | 1986 | 3943898 |
| lysozyme-mediated aggregation and lysis of the periodontal microorganism capnocytophaga gingivalis 2010. | the ability of lysozyme to aggregate and lyse the gram-negative capnophilic periodontal microorganism capnocytophaga gingivalis 2010 was monitored optically at 540 nm. both hen egg white and chromatographically purified human lysozymes had significant but similar aggregation potentials for both logarithmic- and stationary-phase bacteria. in general, an increase in enzyme concentration resulted in a graded increase in both the initial and maximum changes in turbidity which occurred during the rea ... | 1985 | 3967924 |
| relationship of rumen fluid dilution rate to rumen fermentation and dietary characteristics of beef steers. | data from seven beef steer trials were compiled and regression analyses used to evaluate relationships among molar proportions of acetate (ac), propionate (pr) and butyrate (bu), total concentration of volatile fatty acids (vfa), rumen ammonia (nh3), rumen ph, rumen fluid dilution rate (fdr), rumen fluid volume (fvol), body weight (wt), dry matter intake (dmi) and dietary concentration and intake of crude protein (cp and cpi), acid detergent fiber (adf and adfi), ash (ash and ashi) and metaboliz ... | 1985 | 3988654 |
| striatal tyrosine hydroxylases with high and low affinity for tyrosine: implications for the multiple-pool concept of catecholamines. | | 1973 | 4148015 |
| some pharmacological properties of a synthetic oxytocin analogue [1-n-carbamoyl-hemicystine-2-o-methyltyrosine]-oxytocin (carbamoyl-methyloxytocin), an antagonist to the neurohypophysial hormones. | 1. a synthetic oxytocin analogue, [1-n-carbamoyl-hemicystine-2-o-methyltyrosine]-oxytocin (carbamoyl-methyloxytocin), has been tested as an antagonist to the actions of oxytocin and vasopressin on the uterus, the mammary gland and blood pressure.2. the analogue inhibited the response of the isolated rat uterus to both oxytocin and vasopressin without itself stimulating the uterus to contract. the responses to equipotent doses of oxytocin and vasopressin were inhibited equally. there was little o ... | 1970 | 4321760 |
| some pharmacological properties of a synthetic oxytocin analogue [1-n-carbamoyl-hemicystine-2-o-methyltyrosine]-oxytocin (carbamoyl-methyloxytocin), an antagonist to the neurohypophysial hormones. | 1. a synthetic oxytocin analogue, [1-n-carbamoyl-hemicystine-2-o-methyltyrosine]-oxytocin (carbamoyl-methyloxytocin), has been tested as an antagonist to the actions of oxytocin and vasopressin on the uterus, the mammary gland and blood pressure.2. the analogue inhibited the response of the isolated rat uterus to both oxytocin and vasopressin without itself stimulating the uterus to contract. the responses to equipotent doses of oxytocin and vasopressin were inhibited equally. there was little o ... | 1970 | 4321760 |
| inhibition of hepatic ribonuclease activity by chronic administration of phenobarbitone. | | 1970 | 4394767 |
| suppressor cells in the rabbit appendix. | | 1974 | 4547314 |
| homograft tympanic membrane in cats. | | 1974 | 4606619 |
| [right ventricular function (author's transl)]. | | 1973 | 4797309 |
| numerous multiple hairs. | | 1970 | 4989540 |
| sodium fluoride susceptibilities of suspected periodontopathic bacteria. | utilizing a blood-based medium, we determined the minimum inhibitory concentrations (mic's) to sodium fluoride (naf) of 45 bacterial strains representing 22 oral species. bacterial susceptibility ranged from 128 micrograms/ml to 2048 micrograms/ml. of those organisms tested, actinobacillus actinomycetemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga ochracea, and actinomyces viscosus were susceptible at lower levels of naf. | 1983 | 6574154 |
| ultrasonic dispersion of pure cultures of plaque bacteria and plaque. | this study compared the sonic sensitivity of 12 gram-negative and two gram-positive bacteria commonly encountered in plaque associated with periodontal diseases. pure bacterial cultures were grown to standard turbidity, diluted in 1/4 strength prereduced anaerobically sterilized ringer's solution, and aliquots dispersed for 0-180 s, using an mse sonic oscillator at 6 micron under 80% n2, 10% h2 and 10% co2. viable recoveries were determined on anaerobically cultured trypticase soy 5% blood agar ... | 1981 | 6947390 |
| scanning electron-microscopic observations on the in vitro colonization of human dentine by capnocytophaga gingivalis. | | 1982 | 6958369 |
| [endotoxin-like activity in capnocytophaga gingivalis]. | | 1981 | 7035860 |
| cellular fatty acids of capnocytophaga species. | the cellular fatty acid composition of 18 clinical isolates and 4 reference strains of capnocytophaga species (capnocytophaga ochracea, capnocytophaga gingivalis, and capnocytophaga sputigena) was determined by gas-liquid chromatography. the fatty acid profiles of the 22 cultures were essentially identical and were characterized by major amounts (60% or greater) of a saturated, iso-branched-chain, 15-carbon acid (13-methyl-tetradecanoate) and the presence of two relatively uncommon saturated, is ... | 1982 | 7153332 |
| microflora in adult periodontitis. | the subgingival microflora of adult periodontitis was studied in 8 adults (36-47 years) and compared with that of 10 periodontally healthy individuals (36-43 years). a total of 64 periodontal lesions were examined, and classified according to the attachment level in three categories: attachment loss > 6 mm, attachment loss 4-6 mm and attachment loss < 4 mm. also for comparative purposes 20 gingival sulci were evaluated. samples were taken using three standardized paper points and were incubated ... | 1995 | 7494174 |
| detection of porphyromonas gingivalis in oral plaque samples by use of the polymerase chain reaction. | periodontitis is believed to be caused by bacteria which inhabit periodontal pockets. the identification of these periodontal pathogens by currently available methods requires considerable time and expertise. in this study, we have used a polymerase chain reaction (pcr) assay that is quick, relatively simple, and can detect low numbers of a putative periodontal pathogen. primers specific for the fimbrial gene of porphyromonas gingivalis were selected from the published sequence and used for ampl ... | 1993 | 8388414 |
| aspartame as a source of essential phenylalanine for the growth of oral anaerobes. | phenylalanine and aspartic acid requirements were determined for 13 species of oral bacteria using the chemically defined medium omiz-w1. none of actinobacillus actinomycetemcomitans, bacteroides forsythus, eikenella corrodens, selenomonas sputigena, treponema pectinovorum, t. socranskii, or wolinella recta required either of these amino acid constituents of aspartame (l-aspartyl-l-phenylalanine methylester). phenylalanine was essential for the growth of capnocytophaga gingivalis, eubacterium ti ... | 1993 | 8514113 |
| capnocytophaga gingivalis aminopeptidase: a potential virulence factor. | the production and properties of an aminopeptidase from capnocytophaga gingivalis were studied. c. gingivalis was grown in continuous culture over a range of dilution rates and the cell-bound and extracellular levels of aminopeptidase and trypsin-like protease (tlpase) measured. at high growth rates (0.6 mu rel) tlpase specific activity was low and found exclusively as cell-bound activity; at low growth rates (0.0375 mu rel), specific activity was high and 26% was found as extracellular activity ... | 1995 | 8574402 |
| capnocytophaga gingivalis: effects of glucose concentration on growth and hydrolytic enzyme production. | in chemostat culture, the microaerophilic, co2 requiring, gingival-plaque-associated bacterium capnocytophaga gingivalis responded to the addition of glucose (1-6 g i-1) by doubling its growth rate and increasing its biomass yield fivefold. the data suggest that the glucose is catabolized by a fully aerobic route. rather than repressing hydrolytic enzymes which might be associated with pathogenic properties, glucose enhanced the specific activity of aminopeptidase, trypsin-like protease, acid an ... | 1996 | 8760930 |
| hydrolysis of triclosan monophosphate by dental plaque and selected species of oral micro-organisms. | triclosan monophosphate is a phosphorylated derivative of the antimicrobial agent, triclosan. in comparison with triclosan, it is highly soluble in aqueous solutions. it is hypothesized that, within the oral environment, triclosan monophosphate (which may be devoid of antimicrobial activity) will be hydrolyzed into triclosan by the action of microbial phosphatases. the liberated triclosan may then exert antimicrobial activity. to test this hypothesis, we designed experiments to measure the phosp ... | 1996 | 8906126 |
| degradation of lactoferrin by periodontitis-associated bacteria. | the degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. the degradation of lactoferrin was more extensive by porphyromonas gingivalis and capnocytophaga sputigena, slow by capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia, and very slow or absent by prevotella nigrescens, campylobacter rectus ... | 1996 | 8961558 |
| effect of oral bacteria on peripheral blood leukocyte interleukin-6 and soluble interleukin-6 receptor production. | to determine the effect of pathogenic oral bacteria on interleukin 6 (il-6) and soluble il-6 receptor production, we measured their release by human peripheral blood mononuclear cells in vitro. unseparated peripheral blood mononuclear cells, peripheral blood lymphocytes (monocyte depleted), pure t cells, or monocytes were cultured with actinobacillus actinomycetemcomitans, capnocytophaga gingivalis, capnocytophaga ochracea, fusobacterium nucleatum or porphyromonas gingivalis for 24 h. supernatan ... | 1996 | 9028259 |
| rheumatoid factor from periodontitis patients cross-reacts with epitopes on oral bacteria. | the objective of this study was to determine the antigenic specificity of rheumatoid factor (rf) that had previously been reported in the serum of patients with periodontitis. | 1996 | 9171508 |
| hla-d types and serum igg responses to capnocytophaga in diabetes and periodontitis. | serum igg responses to the cell envelope proteins (ceps) from capnocytophaga sputigena, capnocytophaga ochracea, and capnocytophaga gingivalis were examined in periodontally healthy and periodontitis subjects, both with and without type 1 diabetes (n = 60). serum igg responses to ceps were determined by immunoblotting with biotin-goat anti-human igg and an alkaline phosphatase-streptavidin system. reactivity was analyzed by transmission densitometry, digitization, and computer manipulation. the ... | 1997 | 9390475 |
| effects of triclosan and triclosan monophosphate on maximum specific growth rates, biomass and hydrolytic enzyme production of streptococcus sanguis and capnocytophaga gingivalis in continuous culture. | dental plaque species, streptococcus sanguis and capnocytophaga gingivalis, were grown in continuous culture with progressively increasing concentrations of triclosan or its phosphorylated derivative, triclosan monophosphate (tmp). for both organisms, the maximum specific growth rates decreased with increasing concentrations of triclosan or tmp until complete inhibition of growth occurred, which for s. sanguis was at 20 mg/l and 50 mg/l, and for c. gingivalis was at 10 mg/l and 5 mg/l for triclo ... | 1997 | 9421313 |
| characterization of protease activities in capnocytophaga spp., porphyromonas gingivalis, prevotella spp., treponema denticola and actinobacillus actinomycetemcomitans. | protease activities in cell sonicates of defined bacterial strains were examined using peptide substrates and class-specific inhibitors. capnocytophaga spp. all produced serine dipeptidyl peptidase activity and arginine/lysine, elastase- and chymotrypsin-like enzymes with some metalloprotease characteristics. the elastase-like activity was strongest in capnocytophaga sputigena, but the others were greatest in capnocytophaga gingivalis. the latter also had a separate arginine-specific enzyme whic ... | 1997 | 9467394 |
| antimicrobial activity of magnolol and honokiol against periodontopathic microorganisms. | magnolol (1) and honokiol (2), main compounds from the stem bark of magnolia obovata thunb., were evaluated for an antimicrobial activity against periodontopathic microorganisms, porphyromonas gingivalis, prevotella gingivalis, actinobacillus actinomycetemcomitans, capnocytophaga gingivalis, and veillonella disper, and a cytotoxicity against human gingival fibroblasts and epithelial cells. our results indicate that magnolol and honokiol, although less potent than chlorhexidine, show a significan ... | 1998 | 9619121 |
| subgingival microflora associated with nifedipine-induced gingival overgrowth. | the purpose of this study was to examine the composition of subgingival plaque of 140 periodontal lesions in 35 patients with cardiovascular disorders who were administered nifedipine and manifested nifedipine-induced gingival overgrowth (go). age was inversely associated with the go. plaque index and bleeding index showed a significant association with go, while nifedipine dosage and duration of nifedipine therapy were not found to be significant predictors of go. the gingival inflammation as e ... | 1998 | 9660335 |
| growth and hydrolytic enzyme production of capnocytophaga gingivalis on different protein substrates. | capnocytophaga gingivalis was grown with proteins (albumin, collagen, mucin and hemoglobin) as carbon and energy sources in chemostat culture. the mu max (0.34 h-1) and biomass yield (0.96 g.l-1) were as high with hemoglobin (3 g.l-1) as with glucose (3 g.l-1) (20). albumin, collagen and mucin also supported an increased mu max, or yield or both, in comparison with basal (tryptone/thiamine) medium. in steady-state, trypsin-like protease specific activity increased 3- to 5-fold in the presence of ... | 1999 | 10219172 |
| flow cytometry to monitor phagocytosis and oxidative burst of anaerobic periodontopathogenic bacteria by human polymorphonuclear leukocytes. | the reduced susceptibility to phagocytosis found among some periodontopathogenic anaerobes may account for the differences between invasive and non-invasive strains. we applied flow cytometry as a powerful tool to analyze and quantify phagocytosis using standardized cultures of oral anaerobes (porphyromonas gingivalis, prevotella intermedia, p. nigrescens, capnocytophaga gingivalis, c. ochracea, c. sputigena, fusobacterium nucleatum and peptostreptococcus micros) and heparinized whole blood. bac ... | 1999 | 10384401 |
| profile of subgingival microbiota in children with primary dentition. | eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). the samples were cultured for bacterial isolat ... | 2000 | 10791707 |
| [a case report of lung abscess caused by capnocytophaga gingivalis infection]. | a 48-year-old male was admitted to our hospital because of fever, cough and of loss appetite. chest x-p revealed an abnormal shadow in the left upper lobe. bronchoscopy was performed and capnocytophaga gingivalis was cultured from the bronchial lavage and bronchial curreting fluid. ceftizoxime sodium and clindamycin phosphate was administered intravenously. he was discharged after 30 days. he did not have any immunosuppressive underlying disease including hiv infection and diabetes mellitus whic ... | 2000 | 10965664 |
| profile of subgingival microbiota in children with mixed dentition. | a diversity of microbial species has been detected in children's oral flora at an early age. to investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. the samples were cultured for bacterial isolation anaerobica ... | 2000 | 11155173 |
| assessment of periradicular microbiota by dna-dna hybridization. | in the present study the "checkerboard" dna-dna hybridization technique was used to identify bacteria in periapical endodontic lesions of asymptomatic teeth. thirty-four patients with root-filled teeth and apical periodontitis were divided into two groups, each containing 17 patients. in group 1, a marginal incision was performed during surgery to expose the lesion, and in group 2, a submarginal incision was applied. the gingiva and mucosa were swabbed with an 0.2% chlorhexidine gluconate soluti ... | 2000 | 11202881 |
| c-telopeptide pyridinoline cross-links (ictp) and periodontal pathogens associated with endosseous oral implants. | detection of periodontal or peri-implant sites exhibiting progressing disease or those at risk of deterioration has proven difficult. pyridinoline cross-linked carboxyterminal telopeptide of type i collagen (ictp), a marker specific for bone degradation found in gingival crevicular fluid (gcf), has been associated with both bone and attachment loss in periodontitis and may be useful for predicting disease activity. the aim of this cross-sectional study was to examine the relationship between ict ... | 1998 | 11429938 |
| clinical periodontal findings and microflora profiles in children with chronic neutropenia under supervised oral hygiene. | this is the first known case report that used a polymerase chain reaction (pcr)-based method to help identify the oral microflora in patients with chronic neutropenia. in this study, we report clinical periodontal findings and microflora profiles of 2 children, 1 with severe congenital neutropenia (scn, kostmann type) and 1 with cyclic neutropenia (cn). | 2001 | 11495144 |
| association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid. | the objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (ast) in gingival crevicular fluid (gcf) using the colorimetric periogard (ptm) test and the subgingival microflora in early onset periodontitis lesions. | 2001 | 11737506 |
| pneumonia and sepsis due to fluoroquinolone-resistant capnocytophaga gingivalis after autologous stem cell transplantation. | human oral capnocytophaga species have been only rarely described as a cause of sepsis in patients following stem cell or marrow transplantation, and pneumonia has not been reported in this setting. in addition, fluoroquinolone resistance is rarely seen in these species, and has never been reported in c. gingivalis. we report a case of pneumonia (confirmed by culture of bronchoalveolar lavage fluid) and sepsis due to fluoroquinolone- resistant capnocytophaga gingivalis in a patient following aut ... | 2001 | 11803363 |
| microbiological, immunological and genetic factors in family members with periodontitis as a manifestation of systemic disease, associated with hematological disorders. | the microflora, immunological profiles of host defence functions, and human leukocyte antigen (hla) findings are reported for a mother, son and daughter who were diagnosed as having 'periodontitis as a manifestation of systemic diseases, associated with hematological disorders'. examinations were made of the bacterial flora from the periodontal pocket, neutrophil chemotaxis, neutrophil phagocytosis, and the genotypes (dqb1) and serotypes (dr locus) of hla class ii antigens. phenotypic analyses o ... | 2002 | 12200976 |
| capnocytophaga gingivalis bacteremia detected only on quantitative blood cultures in a child with leukemia. | capnocytophaga species are inhabitants of the normal mouth flora. we describe the case of a 6-year-old-girl with leukemia and poor oral hygiene who developed bacteremia caused by capnocytophaga gingivalis. the organism was detected only on quantitative blood cultures. | 2003 | 12613461 |
| subgingival microbiota levels and their associations with periodontal status at the sampled sites in an adult sudanese population using miswak or toothbrush regularly. | little information is available on the effect of miswak use on gingival microbiota. we assessed levels of 28 oral bacteria in subgingival plaque of adult sudanese miswak (n = 38) and toothbrush users (n = 36) age range 20-53 years (mean 34.6 years) to study associations between these bacteria, oral hygiene method, and periodontal status at the sampled sites. a pooled subgingival plaque sample from 6 probing sites of 1 selected tooth in each jaw was obtained from each subject. whole genomic dna p ... | 2003 | 12790510 |
| antibacterial activity of medicinal plant extracts against periodontopathic bacteria. | this study was performed to evaluate the antibacterial activity of althaea officinalis l. roots, arnica montana l. flowers, calendula officinalis l. flowers, hamamelis virginiana l. leaves, illicium verum hook. fruits and melissa officinalis l. leaves, against anaerobic and facultative aerobic periodontal bacteria: porphyromonas gingivalis, prevotella spp., fusobacterium nucleatum, capnocytophaga gingivalis, veilonella parvula, eikenella corrodens, peptostreptococcus micros and actinomyces odont ... | 2003 | 12820224 |
| localized aggressive periodontitis in primary dentition: a case report. | a 5-year-old japanese boy presented with persistent gingival inflammation and severe mobility of the right lower primary incisors. due to severe alveolar bone loss and a deep periodontal pocket (5 mm), the incisors were extracted at the second visit. | 2003 | 12931770 |
| subgingival microbiota of chronic periodontitis subjects from different geographic locations. | most clinical studies assume that the subgingival microbiota is similar from one geographic location to another. the purpose of the present investigation was to examine the composition of the subgingival microbiota in chronic periodontitis subjects from four countries. | 2004 | 15491316 |
| rate of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. | although microbiological studies have identified more than 400 bacterial species in periodontal pockets, only a limited number have been implicated as periodontal pathogens. the purpose of this study was to investigate the incidence of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. bacterial samples were collected with sterile paper points from the deepest periodontal pockets ((5 mm) of 203 patients: 92 males and 111 females, aged 35-55 years. the samples were cul ... | 2004 | 15508748 |
| killing of periodontopathogenic bacteria by photodynamic therapy. | the aim of this study was to evaluate a new approach for killing periodontopathogenic bacteria using photodynamic therapy (pdt). | 2004 | 15562911 |
| differences in the subgingival microbiota of swedish and usa subjects who were periodontally healthy or exhibited minimal periodontal disease. | previous studies have shown differences in the mean proportions of subgingival species in samples from periodontitis subjects in different countries, which may relate to differences in diet, genetics, disease susceptibility and manifestation. the purpose of the present investigation was to determine whether there were differences in the subgingival microbiotas of swedish and american subjects who exhibited periodontal health or minimal periodontal disease. | 2005 | 15642056 |
| isolation and characterization of aminopeptidase from capnocytophaga granulosa atcc 51502. | there is evidence that enzymes from the genus capnocytophaga play a role in dental calculus formation. although most of the species in the genus produce aminopeptidases, there is a paucity of data on the purification and characterization of the enzyme, except in the case of capnocytophaga gingivalis. the aim of this study was to purify aminopeptidase from culture supernatant of capnocytophaga granulosa atcc 51502, a new species of the genus. purification was performed using ammonium sulfate frac ... | 2005 | 15720565 |
| effect of khat chewing on 14 selected periodontal bacteria in sub- and supragingival plaque of a young male population. | the habit of chewing khat (catha edulis) for its amphetamine-like effects is highly prevalent in yemen and east africa, and has expanded to western countries. the purpose of this study was to estimate and compare the prevalence and levels of 14 periodontal bacteria in gingival plaque of khat chewers and khat nonchewers, as well as of khat chewing sides and khat nonchewing sides. | 2005 | 15836514 |
| the salivary microbiota as a diagnostic indicator of oral cancer: a descriptive, non-randomized study of cancer-free and oral squamous cell carcinoma subjects. | the purpose of the present investigation was to determine if the salivary counts of 40 common oral bacteria in subjects with an oral squamous cell carcinoma (oscc) lesion would differ from those found in cancer-free (oscc-free) controls. | 2005 | 15987522 |
| subgingival microflora in smokers with early onset periodontitis. | cigarette smoking is a potent risk factor which has recently been associated with periodontal disease progression. the objective of this study was to detect the microbial profile of early onset periodontitis in smokers and compare it to that of non-smokers. the study population consisted of 50 systemically healthy individuals aged 25 to 38 years, exhibiting early onset periodontitis. 25 patients were smokers (> 20 cigarettes/day) and 25 non-smokers. two pooled bacterial samples comprised of four ... | 1997 | 16887581 |
| association of eubacterium nodatum and treponema denticola with human periodontitis lesions. | the purpose of the present investigation was to compare the levels, proportions and percentage of sites colonized by 40 bacterial species in subgingival plaque samples from periodontally healthy subjects and patients with chronic periodontitis to seek possible pathogens other than the consensus pathogens porphyromonas gingivalis and tannerella forsythia. | 2006 | 16922925 |
| pyogenic arthritis caused by capnocytophaga gingivalis in an immunocompetent three-year-old male. | capnocytophaga gingivalis is most often isolated as normal oral flora or with periodontal disease. this organism is also associated with sepsis usually in immunocompromised hosts. we identified pyogenic arthritis caused by c. gingivalis in a 3-year-old immunocompetent male, whose clinical course closely resembled monoarticular onset pauciarticular juvenile rheumatoid arthritis. this is the first report of c. gingivalis septic arthritis in the world literature, but there are increasing reports of ... | 2001 | 17039147 |
| antimicrobial effect of acidified nitrate and nitrite on six common oral pathogens in vitro. | salivary nitrate is positively correlated with plasma nitrate and its level is 9 times the plasma level after nitrate loading. nitrate in saliva is known to be reduced to nitrite by oral bacteria. nitrate and nitrite levels in saliva are 3 - 5 times those in serum in physiological conditions respectively in our previous study. the biological functions of high salivary nitrate and nitrite are still not well understood. the aim of this in vitro study was to investigate the antimicrobial effects of ... | 2006 | 17134590 |