respiratory mechanisms in the flexibacteriaceae: terminal oxidase systems of saprospira grandis and vitreoscilla species.particles from both saprospira grandis and vitreoscilla species, obtained by high-pressure extrusion and sonic treatment, respectively, actively catalyze the oxidation of reduced nicotinamide adenine dinucleotide (nadh) and succinate with o(2). these activities are inhibited by cyanide but not by antimycin; saprospira is also amytal- and rotenone-insensitive. vitreoscilla preparations were unable to oxidize mammalian ferrocytochrome c and reduced tetramethyl-p-phenylenediamine, whereas the sapro ...19714323292
purification and characterization of the fructose diphosphate aldolases from anacystis is nidulans and saprospira thermalis. 19685636376
rod-shaped ribonucleoprotein particles from saprospira. 196414124863
a phage infecting saprospira grandis. 196414124864
characterization of cytoplasmic fibril structures found in gliding cells of saprospira sp.the cytoplasmic fibril structures of saprospira sp. strain ss98-5 grown on a low-nutrient agar medium were purified from cell lysates treated with triton x-100 and were observed by electron microscopy to be about 7 nm in width and 200-300 nm in length. sds-page of the fibril structures exhibited a single protein band with a molecular mass of 61 kda. a saprospira cytoplasmic fibril protein (scfp), which is a subunit of the fibril structures, was digested with trypsin to oligopeptides and analyzed ...200516333347
phylogenetic analyses and diterpenoid production by marine bacteria of the genus saprospira.the relationship between 16s rrna gene sequence-derived phylogeny and the bacterial production of diterpenoids from 18 isolates of marine bacteria belonging to the genus saprospira was determined. restriction fragment length polymorphism (rflp) analysis of the pcr amplified 16s rrna genes of these isolates indicated four distinct phylotypes. the terpenoid metabolite profiles of each phylotype, determined by liquid chromatography mass spectrometry (lcms) and nuclear magnetic resonance (nmr) analy ...200415386120
new neoverrucosane diterpenoids produced by the marine gliding bacterium saprospira grandis.chemical examination of the culture broth extracts of the marine gliding bacterium saprospira grandis (atcc 23116) has resulted in the isolation of four new diterpenoids of the neoverrucosane class. the structures of the new diterpenoids, compounds 1-4, were assigned by combined spectroscopic methods emphasizing 2d nmr experiments. the relative stereochemistry of 1 was determined by 2d roesy nmr methods, while the absolute stereochemistry was assigned by application of the modified mosher method ...200312828468
algicidal activity and gliding motility of saprospira sp. ss98-5.a marine bacterium, saprospira sp. ss98-5, which was isolated from kagoshima bay, japan, was able to kill and lyse the cells of the diatom chaetoceros ceratosporum. the multicellular filamentous cells of this bacterium captured the diatom cells, formed cell aggregates, and lysed them in an enriched sea water (ess) liquid medium. strain ss98-5 also formed plaques on double layer agar plates incorporating diatom cells. the diatom cell walls were partially degraded at the contact sites with the bac ...200312718397
a partial phylogenetic analysis of the "flavobacter-bacteroides" phylum: basis for taxonomic restructuring.on the basis of small subunit rrna sequence analyses five major subgroups within the flavobacteria-bacteroides phylum have been defined. these are tentatively designated the cytophaga subgroup (comprising largely cytophaga species), the flavobacter subgroup (comprising the true flavobacteria and the polyphyletic genus weeksella), the bacteroides subgroup (comprising the bacteroides and certain cytophaga-like bacteria), the sphingobacter subgroup (which contains the known sphingolipid-producing m ...199211541229
freshwater species of saprospira. 196514323025
phylogeny and in situ identification of a morphologically conspicuous bacterium, candidatus magnospira bakii, present at very low frequency in activated sludge.a morphologically conspicuous bacterium that constituted a very small fraction (< 0.01%) of the total microbial community of activated sludge was enriched and analysed phylogenetically by a combination of cultivation-independent molecular and physical methods. the large, corkscrew-shaped, filamentous bacteria were first detected in municipal activated sludge by light microscopy owing to their unusual rotating gliding motility. various attempts at microbiological enrichment and pure culture isola ...199911207728
phylogenetic heterogeneity within the genus herpetosiphon: transfer of the marine species herpetosiphon cohaerens, herpetosiphon nigricans and herpetosiphon persicus to the genus lewinella gen. nov. in the flexibacter-bacteroides-cytophaga phylum.analysis of the 16s rdna sequences of species currently assigned to the genus herpetosiphon revealed intrageneric phylogenetic heterogeneity. the thermotolerant freshwater species herpetosiphon geysericola is most closely related to the type species herpetosiphon aurantiacus in the chloroflexus subdivision of the green non-sulfur bacteria. the marine species herpetosiphon cohaerens, herpetosiphon nigricans and herpetosiphon persicus, on the other hand, were found to form a cluster with sheathed ...19989734027
saprospira grandis: a flexibacterium that can catch bacterial prey by "ixotrophy" 19979337418
isolation and characterization of tubules and plasma membranes from cytophaga columnaris.tubular structures are released from cells of cytophaga columnaris after lysis of the cells. to determine the nature of these tubules, they were purified and their composition was determined. tubules were isolated after treating cell lysates with 1.0% sodium dodecyl sulfate at ph 8.1, which solubilizes all structural components except tubules. plasma membranes from the same organism were isolated by discontinuous sucrose gradient centrifugation of lysed cells. both tubules and membranes are comp ...19734712571
respiratory chain of colorless algae. ii. cyanophyta.whole cell difference spectra of the blue-green algae, saprospira grandis, leucothrix mucor, and vitreoscilla sp. have one, or at the most 2, broad alpha-bands near 560 mmu. at -190 degrees these bands split to give 4 peaks in the alpha-region for b and c-type cytochromes, but no alpha-band for a-type cytochromes is visible. the nadh oxidase activity of these organisms was shown to be associated with particulate fractions of cell homogenates. the response of this activity to inhibitors differed ...19665932404
polyamine distribution profiles in the eighteen genera phylogenetically located within the flavobacterium-flexibacter-cytophaga complex.cellular polyamines of eighteen genera belonging to the flavobacterium-flexibacter-cytophaga complex were analysed by ion exchange liquid chromatography. homospermidine was the major polyamine in the genera bergeyella, riemerella, ornithobacterium, weeksella, capnocytophaga, polaribacter and psychroflexus belonging to the family flavobacteriaceae. in the family spirosomaceae, runella, spirosoma and flectobacillus species contained spermidine whereas cyclobacterium species contained homospermidin ...200111491527
rna distribution in saprospira grandis: the absence of a highly 2'-o-methylated rna species. 19705479355
saprospira toviformis nov. spec. (flexibacterales) from a new zealand seashore. 19705423285
nucleic acids from saprospira grandis: the absence of 2'-o-methyl rna. 19705413480
rhapidosomes: absence of a highly 2'-o-methylated rna component.rhapidosomes of saprospira grandis do not contain rna that is highly methylated at the 2'-oxygen of the sugar residues. neither is such an rna associated with the particles as a contaminant. a cursory examination of the rhapidosomes of two other marine organisms gave similar results.19695360589
growth and nutrition of saprospira grandis gross (flexibacterales). 19725057391
isolation and some physiological features of saprospira thermalis. 196514290962
the carotenoids of flexibacteria. 2. a new xanthophyll from saprospira grandis. 19665958829
characterization of rhapidosomes of saprospira grandis. 19725015401
morphological characteristics and nomenclature of leptospira (spirochaeta) icterohaemorrhagiae (inada and ido).the present study deals with the morphology and systematic position of the causative agent of infectious jaundice. there are several features which are not found in any of the hitherto known genera of spirochaetoidea which led me to give this organism an independent generic name, leptospira, denoting the peculiar minute elementary spirals running throughout the body. the absence of a definite terminal flagellum or any flagella, and the remarkable flexibility of the terminal or caudal portion of ...191819868227
lewinella agarilytica sp. nov., a novel marine bacterium of the phylum bacteroidetes, isolated from beach sediment.a gram-negative, aerobic, light-orange-coloured, marine bacterium (designated sst-19(t)) was isolated from beach sediment in jeju, korea, and its taxonomic position was determined by means of a polyphasic approach. in a neighbour-joining phylogenetic tree based on 16s rrna gene sequences, strain sst-19(t) was positioned within the family 'saprospiraceae', class sphingobacteria, and formed a consistent cluster with lewinella persica atcc 23167(t) (94.7 % sequence similarity). the levels of 16s rr ...200718048730
aureispira marina gen. nov., sp. nov., a gliding, arachidonic acid-containing bacterium isolated from the southern coastline of thailand.three strains of gliding bacteria, 24(t), 62 and 71, isolated from a marine sponge and algae from the southern coastline of thailand, were studied using a polyphasic approach to clarify their taxonomic positions. a phylogenetic analysis based on 16s rrna gene sequences showed that the three isolates formed a distinct lineage within the family 'saprospiraceae' of the phylum bacteroidetes and were related to members of the genus saprospira. the g+c contents of the isolates were in the range 38-39 ...200617159001
a novel bacterium saprospira sp. strain pdy3 forms bundles and lyses cyanobacteria.a helical filamentous cyanobactericidal bacterium was isolated from dianchi lake, a eutrophic freshwater lake in kunming city of the yunnan province in china using a special solid medium. this species was designated strain pdy3. this bacterium was identified as a novel saprospira sp. on the basis of its morphological characteristics and 16s rdna sequence. strain pdy3 showed apparent group behavior on the solid medium, forming orderly, bundle-like group structures. these bundles moved as groups. ...200616368567
metaproteome analysis of sewage sludge from membrane bioreactors.microbial dynamics and enzymatic activities of activated sludge processes are not completely understood yet. a better understanding about the biology is indispensable for further process optimization. since proteins play a key role as catalysts in sludge processes, a protocol for protein extraction and analysis by 2-d page was established. it is based on phenol extraction of alkaline extracts and on a subsequent precipitation with ammonium sulphate. 2-d protein patterns obtained from different s ...201121604373
the microbial community of ophrydium versatile colonies: endosymbionts, residents, and tenants.ophrydium versatile is a sessile peritrichous ciliate (kingdom protoctista, class oligohymenophora, order peritrichida, suborder sessilina) that forms green, gelatinous colonies. chlorophyll a and b impart a green color to ophrydium masses due to 400-500 chlorella-like endosymbionts in each peritrich. ophrydium colonies, collected from two bog wetlands (hawley and leverett, massachusetts) were analyzed for their gel inhabitants. other protists include ciliates, mastigotes, euglenids, chlorophyte ...199511539474
presence of rhapidosomes in various species of bacteria and their morphological characteristics.rod-shaped structures have been observed in cells of pseudomonas, photobacterium, proteus, and saprospira by use of the negative-contrast stain. these structures, referred to as rhapidosomes, appear to be normal components of these cells. other bacteria including escherichia, salmonella, shigella, klebsiella, micrococcus, bacillus, mycobacterium, rhodospirillum, and hydrogenomonas genera failed to reveal these structures. the rhapidosomes of saprospira were found to consist of two rods, one enca ...19674965198
formation of rhapidosomes in saprospira. 19655879505
5s rrna sequences of representatives of the genera chlorobium, prosthecochloris, thermomicrobium, cytophaga, flavobacterium, flexibacter and saprospira and a discussion of the evolution of eubacteria in general.5s rrna sequences were determined for the green sulphur bacteria chlorobium limicola, chlorobium phaeobacteroides and prosthecochloris aestuarii, for thermomicrobium roseum, which is a relative of the green non-sulphur bacteria, and for cytophaga aquatilis, cytophaga heparina, cytophaga johnsonae, flavobacterium breve, flexibacter sp. and saprospira grandis, organisms allotted to the phylum 'bacteroides-cytophaga-flavobacterium' and relatives as determined by 16s rrna analyses. by using a cluste ...19901693658
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