| aplastic anemia: evidence of immuno-pathomechanisms and successful treatment with absorbed atg. | | 1979 | 161537 |
| effects on milk production associated with consumption of decorticated extracted groundnut meal contaminated with aflatoxin. | | 1979 | 538880 |
| the effects of extrahypothalamic cycloheximide on sexual receptivity in the rat. | | 1979 | 546711 |
| analysis of the glucose transporter content of islet cell lines: implications for glucose-stimulated insulin release. | glucose transport across the plasma membrane of mammalian cells is mediated by a family of homologous proteins. each glucose transporter isoform has a specific tissue distribution which relates to that tissue's demand for glucose. the beta-cells of pancreatic islets are known to express a distinct glucose transporter isoform, termed glut 2, which has a high km for glucose. in this study, we examined the glucose transporter content of normal rat islets and three beta cell lines, beta-tc, hit and ... | 1992 | 1489658 |
| cell surface phenotype of in vitro proliferating lymphocytes in htlv-i-associated myelopathy (ham/tsp). | the in vitro proliferation of peripheral blood lymphocytes (pbls) without any mitogenic stimulation is one of the hallmarks of human t lymphotropic virus type i (htlv-i) infection. recent evidence suggests a difference in the degree of the phenomenon between htlv-i-associated myelopathy/tropical spastic paraparesis (ham/tsp) and asymptomatic htlv-i carriers (ac). in this article, we demonstrated several alterations in the features of the in vitro transformed lymphocytes between patients with ham ... | 1992 | 1560112 |
| pathology of the mucous coat of trout skin during an erosive bacterial dermatitis: a technical advance in mucous coat stabilization for ultrastructural examination. | a fixation regime which combined cryopreservation, freeze drying and vapour fixation with osmium tetroxide, was found to preserve the mucous coat of trout skin for ambient temperature scanning electron microscopy. the regime was used to study changes to the mucous coat of trout skin during a spontaneous outbreak of "columnaris" disease--a common dermatitis of commercial salmonids associated with the bacterial pathogen cytophaga columnaris. infected and damaged regions of skin were covered by a m ... | 1992 | 1602054 |
| health status of salmonids in river systems in natal. iii. isolation and identification of bacteria. | both pathogenic and non-pathogenic bacteria were isolated from fish, both salmonid and non-salmonid, from selected river systems in natal. pasteurella pisicida was isolated for the first time from fish in south africa. the isolation of yersinia ruckeri, aeromonas salmonicida, and edwardsiella tarda were recorded for the first time from fish in natal. a. hydrophila and flexibacter columnaris were found to be widespread throughout the river systems in natal. the streptococcus species which caused ... | 1991 | 1881658 |
| oligonucleotide-directed mutagenesis of psbb, the gene encoding cp47, employing a deletion mutant strain of the cyanobacterium synechocystis sp. pcc 6803. | a mutant strain of the cyanobacterium synechocystis sp. pcc (pasteur culture collection) 6803 has been developed in which psbb, the gene coding for the chlorophyl alpha-binding protein cp47 in photosystem ii (psii), has been deleted. this deletion mutant can be used for the reintroduction of modified psbb into the cyanobacterium. to study the role of a large hydrophilic region in cp47, presumably located on the lumenal side of the thylakoid membrane between the fifth and sixth membrane-spanning ... | 1991 | 1932693 |
| oral prostaglandin e analogues induce intestinal migrating motor complexes after a meal in dogs. evidence for a central mechanism. | the effects of oral, intravenous, and intracerebroventricular administration of synthetic derivatives of prostaglandins e1 (misoprostol) and e2 (enprostil) on postprandial gastrointestinal motility were investigated in dogs chronically fitted with strain gauge transducers on the antrum and the proximal and middle jejunum. synthetic prostaglandin e analogues administered orally at a dose of 20-50 micrograms/kg 15 min before the meal did not modify the postprandial pattern of gastric contractions ... | 1990 | 2107114 |
| kinetics of "p"-site-mediated inhibition of adenylyl cyclase and the requirements for substrate. | the kinetics of "p"-site-mediated inhibition of adenylyl cyclase was studied with the detergent-solubilized enzyme from rat brain. mn2(+)-activated adenylyl cyclase exhibited typical noncompetitive inhibition by 2'-d3'-amp or 2',5'-dideoxyadenosine (2',5'-ddado). however, enzyme that was preactivated with guanosine 5'-o-(3-thiotriphosphate) (gtp gamma s) or proteolytically with ninhibin (+ gtp gamma s) exhibited apparently uncompetitive inhibition with either 2'-d3'-amp or 2',5'-ddado and with e ... | 1990 | 2365688 |
| [mechanisms of thrombin-induced plasminogen activator release]. | the protein kinase c inhibitor h7 (10(-5) mol/l) is able to inhibit the thrombin-induced t-pa release in the isolated perfused pig ear. the thrombin-induced t-pa release can be blocked by increasing the intracellular c-amp via either the activation of adenylate cyclase by means of forskolin, or the inhibition of the phosphodiesterase by means of motapizone or milrinone. protein kinase c is assumed to be involved in the process of thrombin-induced t-pa release. | 1989 | 2483708 |
| a naturally immunogenic virion-associated protein specific for hiv-2 and siv. | the genomic organization of hiv-1 and the family of hiv-2 and siv viruses is similar. however, there is an open reading frame, orf-x, that is present in hiv-2 and siv, but not in hiv-1. the extent of protein sequence conservation in orf-x between hiv-2rod and sivmac suggests that this open reading frame encodes a gene that may be important for infectivity or replication. here, we show that the orf-x products of sivmac and hiv-2sbl-6669 are virion-associated and that the introduction of a prematu ... | 1988 | 2842694 |
| a naturally immunogenic virion-associated protein specific for hiv-2 and siv. | the genomic organization of hiv-1 and the family of hiv-2 and siv viruses is similar. however, there is an open reading frame, orf-x, that is present in hiv-2 and siv, but not in hiv-1. the extent of protein sequence conservation in orf-x between hiv-2rod and sivmac suggests that this open reading frame encodes a gene that may be important for infectivity or replication. here, we show that the orf-x products of sivmac and hiv-2sbl-6669 are virion-associated and that the introduction of a prematu ... | 1988 | 2842694 |
| transmembrane proton-motive potential of spiroplasma floricola. | in spiroplasma floricola, the transmembrane proton-motive potential delta p was studied. it is composed of a transmembrane electric potential difference, delta psi, and a transmembrane proton gradient, delta ph, according to delta p = delta psi - (z.delta ph). using a potential-sensitive carbocyanine dye and 5,5'-dimethyl[2-14c]oxazolidine-2,4-dione as probes, delta psi and delta ph were measured at different [h+] of the medium, and delta p was calculated to be remarkably constant at -123 mv +/- ... | 1987 | 2890538 |
| identification of immunoreactive somatostatin in the rat harderian gland: regulation of its content by growth hormone, beta-adrenergic agonists and calcium channel blockers. | immunoreactive somatostatin (irs) was identified in the male rat harderian gland (hg) by radioimmunoassay. tissue was extracted and a displacement curve performed; there were no significant differences between values obtained with serial dilutions of extracted tissue and those from purified somatostatin standard used in the radioimmunoassay. basal values of hg-irs were found to be in the nanomolar range (10.8 +/- 3.5 ng irs/mg protein). hypophysectomy did not change the hg-irs but, in vivo growt ... | 1988 | 2901739 |
| an immunoassay for the study of dna-binding activities of herpes simplex virus protein icp8. | an immunoassay was used to examine the interaction between a herpes simplex virus protein, icp8, and various types of dna. the advantage of this assay is that the protein is not subjected to harsh purification procedures. we characterized the binding of icp8 to both single-stranded (ss) and double-stranded (ds) dna. icp8 bound ss dna fivefold more efficiently than ds dna, and both binding activities were most efficient in 150 mm nacl. two lines of evidence indicate that the binding activities we ... | 1985 | 2987527 |
| ultrastructural tissue morphometry of the distribution of extracellular matrix vesicles in remodeling rat tibial bone six days after injury. | a study of the distribution of extracellular matrix vesicles on the 6th day of bone healing was performed by methods of transmission electron microscopy combined with computerized morphometry. the vesicles were detected on the electron micrographs and grouped according to their diameters, distance from the calcified front and type. the different types were selected as follows: vesicles with electron-lucent contents, i.e., 'empty'; vesicles with amorphous electron-opaque contents, i.e., 'amorphou ... | 1987 | 3037839 |
| haemolytic uraemic syndrome associated with yersinia enterocolitica infection. | haemolytic uraemic syndrome (hus) associated with yersinia enterocolitica gastroenteritis is reported in a 6-year-old girl. y. enterocolitica of biotype 03 was isolated from the patient's initial stool sample and was subsequently identified as serotype 03 based on the rising agglutinin titres. this paper shows that yersiniosis should be suspected as a possible cause of hus, and investigations should include the measurement of serum agglutinin titres against antigen preparations of the genus yers ... | 1988 | 3153031 |
| [infertility and chlamydia infection]. | | 1988 | 3206475 |
| columnaris infection among cultured nile tilapia oreochromis niloticus. | flexibacter columnaris was isolated from 13 cultured oreochromis niloticus showing respiratory disorders. the isolates developed typical swarming rhizoid colonies on cytophaga agar medium. antibiotic sensitivity test revealed the susceptibility of f. columnaris isolated to oxytetracycline, chloramphenicol and erythromycin. a marked difference in the pathogenicity of seven tested isolates was observed: two were highly virulent, one was moderately virulent and four were avirulent. no experimental ... | 1988 | 3232970 |
| evaluation of prostaglandins as mediators of tubuloglomerular feedback. | orthograde and retrograde microperfusion experiments were conducted in sprague-dawley rats to evaluate the participation of vasoconstrictive eicosanoids as mediators of tubuloglomerular feedback (tgf) signals. retrograde perfusion with 160 microm arachidonic acid (aa) added to a hypotonic solution enhanced the stop-flow pressure (sfp) feedback responses compared with those obtained with the control hypotonic solution (delta sfp, 1.6 +/- 0.4 vs. 10.1 +/- 0.7 mmhg with aa). blockade of thromboxane ... | 1988 | 3364575 |
| [digestibility of macronutrients and nitrogen balance in pregnant rabbits]. | primiparous new zealand white does have been used to establish the weekly differences of several apparent digestibility coefficients (cda) and the nitrogen balance, between pregnant and non-pregnant does, as well as the evolution of the same parameters along the state of gestation. the intake was 180 g/animal and day at the most. the results show that protein followed by dry matter cdas were the most negatively affected in pregnant does with regard to the non-pregnant ones and throughout the ges ... | 1987 | 3448714 |
| vaccine prevention of haemophilus influenzae type b disease: past, present and future. | in little more than half a century we have come a long way toward the eventual elimination of invasive hib disease as a major threat to children in the united states. the haemophilus b polysaccharide vaccine has provided the first tool for large scale public health intervention efforts in specific target groups of children older than 18 months of age who are likely to benefit from immunization. immunization programs using the currently available vaccine represent an interim measure that is likel ... | 1986 | 3511454 |
| ige antibody against surface antigens of leishmania promastigotes in american cutaneous leishmaniasis. | as ige-mediated immune mechanisms participate in the host defence against some types of parasites, we evaluated sera from american cutaneous leishmaniasis (acl) patients for the presence of this antibody against leishmania. using monoclonal antibodies against human ige and an immunoperoxidase staining technique, 48% of the patients sera tested were found to contain ige antibody that bound strongly to leishmania promastigotes. a much lower proportion of sera from non-symptomatic subjects from eit ... | 1986 | 3517764 |
| effect of cyclophosphamide on the proliferation of l 1210 ascites tumour cells and of jejunal crypt cells in the mouse. | cyclophosphamide (cy) does not act in a cell-cycle specific manner, i.e. exclusively on proliferating cells. it also kills non-proliferating cells, as shown by application of cy to l 1210 ascites tumour-bearing mice during plateau phase growth of the tumour. moreover, treatment with cy of l 1210 ascites tumour cells, double-labelled with [3h] and [14c]-thymidine, suggests that cy is not cell cycle phase dependent, but kills cells out of all cycle phases. there is also an extensive cytocidal effe ... | 1986 | 3768935 |
| bone healing stimulated by plasma factor xiii. osteotomy experiments in sheep. | sheep had their right metatarsals osteotomized midshaft and stabilized by plate and screws. one group was injected with 1250 units of factor thirteen for 9 days postoperatively, and a control group received placebo injections. after 8 weeks, the bones were evaluated biomechanically, histomorphologically and by densitometry. the bones of the treated group had a significantly higher tensile strength than the bones of the control animals. the correlation of biomechanical and morphological results d ... | 1985 | 3984704 |
| isolation and characterization of gliding motility mutants of cytophaga columnaris. | | 1973 | 4130440 |
| the fine structure of chondrococcus columnaris. 3. the surface layers of chondrococcus columnaris. | an electron microscope study of the myxobacterium chondrococcus columnaris has revealed the following structures in the peripheral layers of the cells: (1) a plasma membrane, (2) a single dense layer (probably the mucopeptide component of the cell wall), (3) peripheral fibrils, (4) an outer membrane, and (5) a material coating the surfaces of the cells which could be stained with the dye ruthenium red.the ruthenium red-positive material is probably an acid mucopolysaccharide and may be involved ... | 1967 | 4168729 |
| surface structure of gliding bacteria after freeze-etching. | ultrastructural studies of gliding bacteria demonstrate 10- to 11-nm beads on the inner surface of the outer bilayer of cytophaga columnaris. these were not found in myxococcus xanthus. on treatment with glutaraldehyde and ethanol, the beads appear in linear arrays. | 1973 | 4197273 |
| [blood changes in rats fed diets deficient in tocopherol and ubiquinone. 1. cytological and biochemical changes in the blood of vitamin e deficient rats]. | | 1973 | 4206707 |
| [influence of temperature on the afferent and efferent motor innervation of the spinal cord. ii. temperature dependence of muscle spindle function]. | | 1969 | 4240615 |
| [the complementarity of the receptor structure of bacteriophage and the sensitivity of microbial cells]. | | 1968 | 4249249 |
| the isolation of viruses from acute respiratory infections. v. the use of organ cultures of human embryonic nasal and tracheal ciliated epithelium. | | 1966 | 4292507 |
| the isolation of viruses from acute respiratory infections. v. the use of organ cultures of human embryonic nasal and tracheal ciliated epithelium. | | 1966 | 4292507 |
| antibiotic additives for the isolation of chondrococcus columnaris from fish. | | 1969 | 4304764 |
| studies on enzymes involved in the catabolism of phospholipids in escherichia coli. | | 1973 | 4350652 |
| proceedings: the graded enzymic immaturity of transplanted neoplasms. | | 1974 | 4366507 |
| in vitro testosterone biosynthesis in the human fetal testis. ii. stimulation by cyclic amp and human chorionic gonadotropin (hcg). | | 1974 | 4372039 |
| suppression by noradrenaline of catecholamine secretion from adrenal medulla. | | 1974 | 4423752 |
| [the morphology of the cns in carbon tetrachloride poisoning. histological findings in the telencephalon and in the diencephalon of rabbits treated with carbon tetrachloride. ii]. | | 1973 | 4522593 |
| [the morphology of the cns in carbon tetrachloride poisoning. histological findings in the telencephalon and in the diencephalon of rabbits treated with carbon tetrachloride. ii]. | | 1973 | 4522593 |
| [richard von volkmann]. | | 1973 | 4588173 |
| surface topography of the escherichia coli ribosome enzymatic iodination of the 50s subunit. | | 1974 | 4589314 |
| the identification of f actin of the pollen tube and protoplast of amaryllis belladonna. | | 1974 | 4609784 |
| the action of some anticonvulsant drugs on cobalt-induced epilepsy and on the bemegride threshold in alert cats. | | 1972 | 4626570 |
| the effect of allogeneic demineralized dentin as a pulp capping agent in java monkeys. | | 1972 | 4628280 |
| [variations of thyroid activity as a function of illumination in the rabbit]. | | 1972 | 4662973 |
| seasonal prevalence of chondrococcus columnaris infection in black bullheads from clear lake, iowa. | | 1973 | 4701177 |
| isolation and characterization of tubules and plasma membranes from cytophaga columnaris. | tubular structures are released from cells of cytophaga columnaris after lysis of the cells. to determine the nature of these tubules, they were purified and their composition was determined. tubules were isolated after treating cell lysates with 1.0% sodium dodecyl sulfate at ph 8.1, which solubilizes all structural components except tubules. plasma membranes from the same organism were isolated by discontinuous sucrose gradient centrifugation of lysed cells. both tubules and membranes are comp ... | 1973 | 4712571 |
| different patterns of induction of the two isozymes of alanine aminotransferase of liver of rat as a function of age. | | 1974 | 4826465 |
| [treatment of bacterial pneumonia in children by rondomycin-tao]. | | 1967 | 4883809 |
| purification from calf thymus of an inhibitor of deoxyribonuclease i. | | 1970 | 4909306 |
| [kinetics of reactions catalyzed by escherichia coli polynucleotide phosphorylase. 2-deoxy-adp as substrate and inhibitor]. | | 1970 | 4919419 |
| [effect of the contralateral kidney on compensatory kidney hypertrophy in animal experiments]. | | 1970 | 4931732 |
| [toxigenic properties of the freshly isolated diphtheria cultures]. | | 1965 | 4959235 |
| [the possibility of infecting marals with human type tuberculosis mycobacteria]. | | 1970 | 4989189 |
| immune response of salmonids and exposure of river fishes to chondrococcus columnaris. bnwl-480. | | 1966 | 5301044 |
| oral immunization of juvenile coho salmon against chondrococcus columnaris. bnwl-1050. | | 1970 | 5311306 |
| [studies on the feedback regulation of pulsatile luteinizing hormone secretion: effect of ovarian steroid implantation into the forebrain and basal hypothalamus on pulsatile lh secretion in ovariectomized rats]. | recent studies have demonstrated that ovarian steroids are involved in the regulation of pulsatile lh secretion. in order to identify the site of action of ovarian steroids in modulating pulsatile lh secretion, the effect of local administration of estradiol benzoate (eb) or progesterone (p) into various brain regions on the characteristic of lh pulses was investigated in ovariectomized rats. female rats of the wistar strain were ovariectomized about 3-4 weeks before the experiment. blood sample ... | 1985 | 3996684 |
| [studies on the feedback regulation of pulsatile luteinizing hormone secretion: effect of ovarian steroid implantation into the forebrain and basal hypothalamus on pulsatile lh secretion in ovariectomized rats]. | recent studies have demonstrated that ovarian steroids are involved in the regulation of pulsatile lh secretion. in order to identify the site of action of ovarian steroids in modulating pulsatile lh secretion, the effect of local administration of estradiol benzoate (eb) or progesterone (p) into various brain regions on the characteristic of lh pulses was investigated in ovariectomized rats. female rats of the wistar strain were ovariectomized about 3-4 weeks before the experiment. blood sample ... | 1985 | 3996684 |
| [insulin-like effects of anti-insulin-receptor antibodies on cultured rat hepatocytes]. | the effects of two anti-insulin-receptor antibodies (aira) on 125i-insulin binding, glycogenesis, amino-isobutyric acid (aib) transport and insulin receptor down-regulation on cultured rat hepatocytes were examined. two aira blocked insulin receptor dose-dependently and caused a decrease in available insulin receptor on hepatocytes. aira mimicked insulin action on glycogenesis both in short-term (2 h) and in long-term (24 h, 48 h) experiments, while aira did not mimic insulin action on aib trans ... | 1985 | 3894188 |
| [operation of breast milk centers in finland]. | | 1987 | 3649433 |
| multiple and complex effects of buspirone on central dopaminergic system. | the effects of the anxiolytic drug buspirone and its metabolite 1-pp on the dopaminergic system were investigated. a single buspirone administration was found to decrease da levels and increase its metabolite dopac in striatal samples. the levels of the other da metabolite, 3mt, were unaffected; however its formation rate after inhibition of its metabolism, was found to be increased by buspirone. 1-pp did not affect either dopac or 3mt levels and formation. striatal microdialysis showed that bus ... | 1988 | 3413206 |
| autoantibodies in paraneoplastic cerebellar degeneration bind to cytoplasmic antigens of purkinje cells in humans, rats and mice and are of multiple immunoglobulin classes. | purkinje cell autoantibodies in sera of six female patients with paraneoplastic cerebellar degeneration (pcd) were investigated by indirect immunofluorescence for immunoglobulin isotype and species specificity. four patients had gynecologic cancer, one breast cancer and one lymphoma. control sera from 125 patients were negative for purkinje cell antibodies of igg, igm and iga isotypes. these included neurologically normal cancer patients, cancer patients with paraneoplastic syndromes not involvi ... | 1988 | 3346369 |
| application of transmission electron microscopy to human tumor diagnosis: an historical perspective. | | 1987 | 3322513 |
| heredity and blood pressure. | | 1989 | 2675240 |
| the survival of chondrococcus columnaris in waters of different quality. | | 1968 | 5752923 |
| bacteriophage infecting the myxobacterium chondrococcus columnaris. | kingsbury, david t. (university of washington, seattle), and erling j. ordal. bacteriophage infecting the myxobacterium chondrococcus columnaris. j. bacteriol. 91:1327-1332. 1966.-during a series of screening experiments, seven bacteriophages which infect the pathogenic myxobacterium chondrococcus columnaris were isolated. of these, one was chosen for detailed study. this phage has a wide host range among strains of c. columnaris, but does not infect other myxobacterial species tested. morpholog ... | 1966 | 5929758 |
| galactosamine glycan of chondrococcus columnaris. | the marked viscosity of liquid cultures of the myxobacterium chon-rococcus columnaris is caused by production of an extracellular polysaccharide. the polysaccharide is a high-molecular-weight homopolymer of d-galactosamine in which the galactosamine subunits are connected by (alpha)-(l-->4) glycosidic linkages. half of the amino groups are acetylated. | 1966 | 5939534 |
| the fine structure of chondrococcus columnaris. ii. structure and formation of rhapidosomes. | when cells of c. columnaris were broken open, treated with pta, and examined in the electron microscope, tubular structures (rhapidosomes) were present in the preparations. the rhapidosomes are approximately 300 a in diameter. their length varies from about 500 to about 15,000 a. an axial hole which runs the length of the rhapidosomes appears to widen and narrow with a regular periodicity. end-on views of short segments of rhapidosomes revealed the presence of subunits around their outside perip ... | 1967 | 6061714 |
| the fine structure of chondrococcus columnaris. i. structure and formation of mesosomes. | cells of chondrococcus columnaris were sectioned and examined in the electron microscope after fixation by two different methods. after fixation with osmium tetroxide alone, the surface layers of the cells consisted of a plasma membrane, a dense layer (mucopeptide layer), and an outer unit membrane. the outer membrane appeared distorted and was widely separated from the rest of the cell. the intracytoplasmic membranes (mesosomes) appeared as convoluted tubules packaged up within the cytoplasm by ... | 1967 | 6061716 |
| presence of antigen sensitized leukocytes in carp (cyprinus carpio l) following bath immunization against flexibacter columnaris. | bath immunization of carp (cyprinus carpio l) resulted in protection of fish at natural challenge. stimulation of leukocytes derived from thymus, spleen, anterior kidney and mid-kidney of fish immunized with flexibacter columnaris bacterin revealed the presence of antigen sensitized cells in all lymphoid tissues except the anterior kidney. after 28 days a response was obtained in thymus and spleen leukocyte cultures. | 1982 | 6817508 |
| mixed infection in columnaris disease of fish. | in addition to flexibacter columnaris, corynebacterium sp was recovered from lesions associated with columnaris disease in catfish. catfish exposed to pure cultures of the corynebacterium sp developed circumscribed lesions, which regressed without further adverse effects. disease signs were not demonstrable in catfish exposed to pure cultures of f columnaris; however, when catfish were exposed to mixed cultures of th corynebacterium sp and f columnaris, signs of columnaris appeared and the fish ... | 1980 | 7192703 |
| examination of gills from salmonids with bacterial gill disease using monoclonal antibody probes for flavobacterium branchiophilum and cytophaga columnaris. | bacterial diseases of the gills of commercially reared salmonids in freshwater are common problems. they accounted for 18% of all diagnostic submissions to the atlantic veterinary college from commercial fish hatcheries. definitive diagnosis is difficult because of the growth characteristics of the putative bacteria in culture. research into the pathogenesis of these diseases has also been similarly limited. monoclonal antibodies (mabs) were developed to 2 globally significant gill bacterial pat ... | 1995 | 8580172 |
| shieh medium supplemented with tobramycin for selective isolation of flavobacterium columnare (flexibacter columnaris) from diseased fish. | tobramycin was found to be less inhibitory to flavobacterium columnare (formerly flexibacter columnaris) than to other fish-associated bacteria. the selective capacity of shieh medium, an isolation medium described for this species, was markedly enhanced by adding tobramycin at a concentration of 1 microgram/ml. | 1997 | 8968939 |
| [recent knowledge on fish pathogenic bacteria aeromonas salmonicida, listonella anguillara and cytophaga columnaris, and their virulence factors]. | | 1997 | 9155200 |
| immunization with bacterial antigens: flavobacterium and flexibacter infections. | seven bacterial species belonging to the flavobacterium-cytophaga group are currently considered to be pathogenic for fish. because they were only recently described and/or because the disease they provoke has little economic significance, no study has been performed concerning immunization against chryseobacterium scophthalmum, flavobacterium johnsoniae and flexibacter ovolyticus. immunization with flexibacter maritimus has not been investigated, and fish surviving a natural infection appear to ... | 1997 | 9270847 |
| comparison of whole-cell antigens of pressure- and formalin-killed flexibacter columnaris from channel catfish (ictalurus punctatus). | to identify and compare immunodominant antigens in whole-cell lysates of pressure- and formalin-killed flexibacter columnaris. | 1997 | 9285002 |
| characterization of four flavobacterium columnare (flexibacter columnaris) strains isolated from tropical fish. | four flavobacterium columnare strains (ajs 1-4) were isolated from black mollies (poecilia sphenops) and platies (xiphophorus maculatus), showing white spots on the back, head and skin ulcers. the isolates developed characteristic rhizoid yellow pigmented colonies on shieh agar and typical growth in shieh broth. they were gram-negative, filamentous bacteria exhibiting flexing movements. when compared to f. columnare strains isolated from temperate fish, it was noted that the four strains origina ... | 1998 | 9659690 |
| inducible expression of green fluorescent protein within channel catfish cells by a cecropin gene promoter. | the activity of an insect promoter of the cecropin b gene (cec b) was investigated using green fluorescent protein (gfp) as a reporter in cells of channel catfish (ictalurus punctatus). the expression vector pqz-1 containing the cec b promoter and a modified gfp cdna sequence was delivered by lipofection to three catfish types: fibroblast and leukocyte cell lines, and primary cultures of leukocytes. no resistance genes were included in the vector for selection of gfp-expressing cells. the gfp mr ... | 1998 | 9766968 |
| pharmacokinetics of oxytetracycline in the red pacu (colossoma brachypomum) following different routes of administration. | oxytetracycline (otc) pharmacokinetics were studied in the red pacu (colossoma brachypomum) following intravenous (i.v.) and intramuscular (i.m.) administration at a dose of 5 mg/kg body weight. otc plasma concentrations were determined by high-performance-liquid-chromatography (hplc). a non-compartmental model was used to describe plasma drug disposition after otc administration. following i.m. administration, the elimination half-life (t1/2) was 62.65 +/- 1.25 h and the bioavailability was 49. ... | 1998 | 9811436 |
| flavobacterium hibernum sp. nov., a lactose-utilizing bacterium from a freshwater antarctic lake. | four freshwater antarctic lakes were examined for the presence of beta-galactosidase-producing bacteria using mineral medium enrichments and lactose. enrichments from only one of the lakes produced growth and two strains were isolated that were very similar in phenotype and fatty acid profile, and shared considerable homology in their dna (dna-dna hybridization = 93 +/- 7%). the strains were psychrotrophic with theoretical tmax, tmin and topt of 30-31, -7 degrees and 26 degrees c, respectively. ... | 1998 | 9828443 |
| the association of flavobacterium columnare strains of high and low virulence with gill tissue of black mollies (poecilia sphenops). | the ability of a high virulence strain (ajs 1) and a low virulence strain (ajs 4) of flavobacterium columnare (flexibacter columnaris) to attach to the gills of black mollies (poecilia sphenops) was investigated. for that purpose, two groups of 25 black mollies each were immersed in a bath containing 10(6) cfu/ml of f. columnare ajs 1 or ajs 4. at regular intervals from 1 to 12 h after the contact infection, fish were sacrificed and gills, skin, spleen and heart were sampled for bacteriology. sa ... | 1999 | 10466504 |
| flavobacterium frigidarium sp. nov., an aerobic, psychrophilic, xylanolytic and laminarinolytic bacterium from antarctica. | a psychrophilic, aerobic bacterium designated a2it was isolated from marine sediment recovered from shallow waters surrounding adelaide island, antarctica (67 degrees 34' s, 68 degrees 07' w). the organism exhibited xylanolytic and laminarinolytic activity and was halotolerant. basic characterization showed that it was gram-negative, non-motile, yellow-pigmented (beta,beta-carotene-3,3'-diol) and positive for oxidase and catalase synthesis. analysis of the 16s rdna sequence suggests that the org ... | 2001 | 11491319 |
| partitioning of bacterial communities between seawater and healthy, black band diseased, and dead coral surfaces. | distinct partitioning has been observed in the composition and diversity of bacterial communities inhabiting the surface and overlying seawater of three coral species infected with black band disease (bbd) on the southern caribbean island of curaçao, netherlands antilles. pcr amplification and sequencing of bacterial 16s rrna genes (rdna) with universally conserved primers have identified over 524 unique bacterial sequences affiliated with 12 bacterial divisions. the molecular sequences exhibite ... | 2002 | 11976091 |
| flavobacterium columnare (flexibacter columnaris) associated with severe gill necrosis in koi carp (cyprinus carpio l). | | 2002 | 12074240 |
| diagnosis of flavobacteriosis by direct amplification of rrna genes. | a broad-range bacterial pcr method with universal 16s rdna targeting primers and bacterial cultivation was used to identify the putative pathogen in flavobacterial outbreaks. restriction fragment length polymorphism (pcr-rflp) analysis and sequencing of the partial 16s rdna pcr products of 10 skin samples and 10 representative isolates derived from the same fish specimens revealed differences between direct molecular and cultivation-based analysis. flavobacterium columnare-like sequences dominat ... | 2002 | 12363090 |
| rapid detection of columnaris disease in channel catfish (ictalurus punctatus) with a new species-specific 16-s rrna gene-based pcr primer for flavobacterium columnare. | a 16-s rrna gene from the chromosomal dna of the fish-pathogenic bacterium flavobacterium columnare (formerly flexibacter columnaris), strain ars-i, was cloned, sequenced and used to design a polymerase chain reaction (pcr) primer set. the primer set amplified a specific 1193-bp dna fragment from f. columnare strains but not from related bacteria, f. psychrophilum, f. aquatile, f. branchiophilum, or other bacterial pathogens of fish, flexibacter maritimus, cytophaga johnsonae, edwardsiella ictal ... | 2003 | 12459241 |
| flavobacterium columnare infections in fish: the agent and its adhesion to the gill tissue. | flavobacterium columnare is the cause of columnaris disease, a serious condition affecting numerous freshwater fish species all over the world. hitherto, only very scarce information is available on the pathogenesis of this bacterial disease, making it difficult to adopt a preventive approach to combat this pathogen. this study ambiates to shed more light on the way f. columnare interacts with its host. since a number of difficulties occur in trying to isolate f. columnare from diseased fish, fi ... | 2002 | 12649933 |
| structural characterization of the lipopolysaccharide o-polysaccharide antigen produced by flavobacterium columnare atcc 43622. | the structure of the antigenic o-chain polysaccharide of flavobacterium columnare atcc 43622, a gram-negative bacterium that causes columnaris disease in warm water fish, was determined by high-field 1d and 2d nmr techniques, ms, and chemical analyses. the o-chain was shown to be an unbranched linear polymer of a trisaccharide repeating unit composed of 2-acetamido-2-deoxy-d-glucuronic acid (d-glcnaca), 2-acetamidino-2,6-dideoxy-l-galactose (l-fucnam) and 2-acetamido-2,6-dideoxy-d-xylo-hexos-4-u ... | 2003 | 12899701 |
| study of a bacteriophage infecting the myxobacterium chondrococcus columnaris. | | 1955 | 13271323 |
| studies on the myxobacterium chondrococcus columnaris. i. serological typing. | | 1959 | 13672906 |
| studies on the myxobacterium chondrococcus columnaris. ii. bacteriocins. | | 1959 | 13672907 |
| comparative challenge model of flavobacterium columnare using abraded and unabraded channel catfish, ictalurus punctatus (rafinesque). | the early entry of the fish pathogen flavobacterium columnare and enhancement by abrasion was studied in channel catfish, ictalurus punctatus (rafinesque), using the polymerase chain reaction and a species-specific primer set for a bacterial 16s rrna gene product. evaluations were conducted following an abrasion bath immersion challenge with f. columnare. abrasion, a practice which has historically been used prior to bacterial challenge, had significant effects on the early entry of the pathogen ... | 2003 | 14513970 |
| modifications to the fathead minnow (pimephales promelas) chronic test method to remove mortality due to pathogenic organisms. | whole effluent toxicity testing is used to evaluate the discharge of materials that may be harmful to indigenous aquatic life. unlike most environmental analyses, receiving water (the water body into which the effluent is discharged) often is used as dilution water in whole effluent toxicity tests to simulate the aquatic environment into which the effluent is introduced. in approximately 26% of whole effluent toxicity tests conducted by wisconsin (usa) certified labs between 1988 and 1998, a pat ... | 2003 | 14552005 |
| feed deprivation of channel catfish, ictalurus punctatus (rafinesque), influences organosomatic indices, chemical composition and susceptibility to flavobacterium columnare. | withholding feed has been suggested as a strategy to manage infectious disease of channel catfish, ictalurus punctatus (rafinesque). in a previous study, we demonstrated that deprivation of feed for as little as 7 days reduced innate resistance of catfish to flavobacterium columnare. this study was conducted to evaluate the effect of feeding regimens [no feeding (nf), fed once every other day to satiation (feod) and fed once daily to satiation (fd)] on organosomatic indices, physiological change ... | 2003 | 14575373 |
| the structure of the glycopeptides from the fish pathogen flavobacterium columnare. | proteolytic digestion of the phenol-water extraction product of the fish pathogen flavobacterium columnare afforded a mixture of glycopeptides in which the oligosaccharide moiety was an unusual hexasaccharide composed of 4-o-methyl-2-acetamido-2-deoxy-d-glucuronic acid (glcnaca), d-glucuronic acid (d-glca), 2,3-di-o-acetyl-d-xylose (d-xyl), 2-o-methyl-d-glucuronic acid (d-glca), d-mannose (d-man), and 2-o-methyl-l-rhamnose (l-rha). by the application of high-resolution 1d and 2d nmr, mass spectr ... | 2003 | 14670723 |
| enhanced bacterial disease resistance of transgenic channel catfish ictalurus punctatus possessing cecropin genes. | the cecropin b gene from the moth hyalophora cecropia, driven by the cytomegalovirus promoter, was transferred to the channel catfish ictalurus punctatus. transgenic individuals (p1) were mated to produce individuals (f1) that exhibited enhanced disease resistance and survival when challenged with pathogenic bacteria. during the epizootic of flavobacterium columnare in an earthen pond, the percentage of transgenic individuals containing preprocecropin b construct that survived (100%) was signifi ... | 2002 | 14961267 |
| acute columnaris infection in channel catfish, ictalurus punctatus (rafinesque): efficacy of practical treatments for warmwater aquaculture ponds. | columnaris disease was induced in channel catfish, ictalurus punctatus (rafinesque), by bath exposure to four highly virulent isolates of flavobacterium columnare. in untreated controls, mortality began 20 h after exposure and reached 100% by 48 h. mortality in channel catfish given antibiotic treatments with oxytetracycline or a combination of sulphadimethoxine and ormetoprim in feed prior to bacterial challenge was zero with all four strains of f. columnare. diquat (zeneca agricultural product ... | 2004 | 14986936 |
| morphological and genetic characteristics of flavobacterium columnare isolates: correlations with virulence in fish. | variability in pathogenicity of flavobacterium columnare makes disease treatment difficult because there is currently no way to easily recognize those strains that warrant aggressive treatments. in order to identify suitable virulence markers, 17 isolates of f. columnare were cultured from six different fish species. the dna from all isolates was analysed using random amplified polymorphic dna (rapd). bootstrap analysis of the rapd data produced a tree with three major groups supported by bootst ... | 2004 | 14986937 |