Publications

TitleAbstractYear
Filter
PMID
Filter
croup syndrome in children with influenza. 197722186
conformational study of calf brain tubulin. 197823729
projections of auditory nerve in the cat as seen by anterograde transport methods. 197991142
viral specificity of h-2-restricted t killer cells directed against syngeneic tumors induced by gross, friend, or rauscher leukemia virus.cytolytic t lymphocytes (ctl) were generated against murine tumors induced by gross, friend, or rauscher leukemia virus (lv) in syngeneic mixed leukocyte-tumor cell cultures. analogous to the patterns of specificity observed with antibodies to lv-induced cell surface antigens, ctl could be classified into two major groups of specificity. tumor cells induced by friend, moloney, or rauscher virus and positive for the fmr antigen were killed by syngeneic ctl immune to any one of these three lv; the ...197991655
plasmodium: experimental animals for human malaria and research needs. a review. 1978103748
polychlorinated biphenyl-induced morphologic changes in the gastric mucosa of the rhesus monkey.the early morphologic events in polychlorinated biphenyl-induced lesions of the gastric mucosa of immature male rhesus monkeys (macaca mulatta) were studied in serial biopsies taken during consumption of diets containing 3, 10, 30, and 100 mg. per kg. (p.p.m.) of aroclor 1242. the severity of the lesions correlated directly with both the duration and the level of exposure. the first changes were seen after ingestion of 30 and 100 mg. per kg. for 2 weeks; changes were not seen until 10 weeks at 3 ...1979106183
[determinaton of intracellular immunoglobulins by means of immunofluorescence. 1. the standardization of monospecific luminescent antisera against human g-, a- and m-class immunoglobulins in bone marrow preparations from patients with paraproteinemic hemoblastosis].to assess the specificity and the activity of fluorescent antisera against human g-, a-, m-classes the authors used in the capacity of a substrate, containing specific antigen preparations, bone marrow cells of patients with paraproteinemic hemoblastosis. the method of making the preparations used permitted sufficiently objective assessment of the activity and specificity of the mentioned monospecific fluorescent conjugates. as revealed, the bone marrow preparations could be stored at -70 degree ...1978106607
iso-branched 2- and 3-hydroxy fatty acids as characteristic lipid constituents of some gliding bacteria.the fatty acids present in the total hydrolysates of several gliding bacteria (myxococcus fulvus, stigmatella aurantiaca, cytophaga johnsonae, cytophaga sp. strain samoa and flexibacter elegans) were analyzed by combined gas-liquid chromatography and mass spectrometry. in addition to 13-methyl-tetradecanoic acid, 15-methyl-hexadecanoic acid, hexadecanoic acid, and hexadecenoic acid, 2- and 3-hydroxy fatty acids comprised up to 50% of the total fatty acids. the majority was odd-numbered and iso-b ...1979118159
[study of the antibiotic sensitivity of the shigellae, pathogenic escherichia, proteus and staphylococcus isolated from young children in tbilisi with acute intestinal diseases].sensitivity of shigella, pathogenic escherichia, proteus and staphylococcus isolated from children with acute intestinal diseases was studied with respect to antibiotics widely used in medical practice: streptomycin, levomycetin, tetracycline, chlortetracycline, oxytetracycline and neomycin. the antibiotic sensitivity was determined with the method of indicator discs. it was found that sensitivity of shigella was: 44 percent to streptomycin, 69.5 per cent to levomycetin, 18.5 per cent to tetracy ...1975123726
purification by affinity chromatography, properties and crystallisation of phosphofructokinase from thermophilic micro-organisms. 1975124670
aplastic anemia: evidence of immuno-pathomechanisms and successful treatment with absorbed atg. 1979161537
peroxidase-conjugate chromatography isolation of conjugates prepared with glutaraldehyde or periodate using polyacrylamide-agarose gel.fractionation abilities of polyacrylamide-agarose gel (ultrogel) and dextran gel (sephadex) column chromatography were compared in isolating horseradish peroxidase conjugates, prepared using two different methods. utrogel aca-44 provides an efficient separation of monomer conjugated and nonconjugated immunoglobulins resulting from the two-step glutaraldehyde procedure, sephadex g-200 does not. both types of columns eluted the polymer conjugates resulting from the periodate procedure in the void ...1976177698
conformations of a,t-rich dnas.dnas from the genomes of clostridium perfringens and cytophaga johnsonii display orthodox a-dna and b-dna structures despite their high (a+l) nucleotide content. unique structures, such as those found for synthetic dnas having specific special sequences, do therefore not necessarily occur for dnas having more random base sequence even if these have unusual base compositions. clostridium perfringens dna exhibits unusual structural properties only prior to purification by gel filtration.1976186759
relation of plasma high-density lipoprotein cholesterol to lipoprotein-lipase activity in adipose tissue and skeletal muscle of man.lipoprotein-lipase activity (lpl) was measured in biopsies of adipose tissue and skeletal muscle of normal human subjects, and the results were related to concentrations of cholesterol and triglycerides in plasma lipoproteins. adipose-tissue lpl activity was significantly higher in females than in males, whereas no sex difference was observed in skeletal-muscle lpl activity. a highly significant positive correlation was present between the plasma high density lipoprotein (hdl) cholesterol level ...1978208590
disruptions in enzyme regulation during aging. 1975239674
analysis of mitotic nondisjunction with aspergillus nidulans.two methods to detect the induction of nondisjunction with a diploid stable strain of a. nidulans are described. the first method gives only qualitative results, while the second method is quantitative and dose-effect curves can be done. some physiological parameters affecting the induction of nondisjunction can also be studied, because either quiescent or germinating conidia can be treated with the drug under test. some agents inducing nondisjunction were also tested for the induction of point ...1979387402
g1499-2, a new quinoline compound isolated from the fermentation broth of cytophaga johnsonii.a new quinoline compound, g1499-2[c18h21no(i)] is produced by cytophaga johnsonii. g1499-2 has an unusual structure containing a cyclopropylidene radical. the compound has limited antibiotic activity against a few bacteria. it is not toxic to mice.1978711619
bacterioplankton community structure and dynamics after large-scale release of nonindigenous bacteria as revealed by low-molecular-weight-rna analysis.a set of freshwater mesocosms (1.7 m3 each) was inoculated with large amounts of escherichia coli, pseudomonas putida, and their culture medium to substantially disturb the natural microbial community. to monitor microbial community dynamics, low-molecular-weight rna (5s rrna and trna) obtained directly from bacterioplankton was analyzed by using high-resolution electrophoresis. the introduced bacteria showed no significant effect on the community structure of the natural bacterial assemblage an ...19921280060
is enough done to track hiv infections? 19921317606
opposite effects of dimethyl sulfoxide and ethanol on synaptic membrane fluidity.dimethyl sulfoxide (dmso) is an organic solvent with myriad biological actions, including actions on synaptic membrane transport processes. in this study, fluorescence polarizations of the probes diphenylhexatriene (dph: a probe of the hydrophobic membrane core), trimethylammonium-diphenylhexatriene (a probe of the superficial domain of the cytofacial synaptic membrane leaflet) and diphenylhexatriene propionic acid (a probe of the superficial domain of the exofacial synaptic plasma membrane leaf ...19921472307
analysis of the glucose transporter content of islet cell lines: implications for glucose-stimulated insulin release.glucose transport across the plasma membrane of mammalian cells is mediated by a family of homologous proteins. each glucose transporter isoform has a specific tissue distribution which relates to that tissue's demand for glucose. the beta-cells of pancreatic islets are known to express a distinct glucose transporter isoform, termed glut 2, which has a high km for glucose. in this study, we examined the glucose transporter content of normal rat islets and three beta cell lines, beta-tc, hit and ...19921489658
origin and evolution of the genus homo.it is remarkable that the taxonomy and phylogenetic relationships of the earliest known representatives of our own genus, homo, remain obscure. advances in techniques for absolute dating and reassessments of the fossils themselves have rendered untenable a simple unilineal model of human evolution, in which homo habilis succeeded the australopithecines and then evolved via h. erectus into h. sapiens-but no clear alternative consensus has yet emerged.19921538759
5s rrna sequences of representatives of the genera chlorobium, prosthecochloris, thermomicrobium, cytophaga, flavobacterium, flexibacter and saprospira and a discussion of the evolution of eubacteria in general.5s rrna sequences were determined for the green sulphur bacteria chlorobium limicola, chlorobium phaeobacteroides and prosthecochloris aestuarii, for thermomicrobium roseum, which is a relative of the green non-sulphur bacteria, and for cytophaga aquatilis, cytophaga heparina, cytophaga johnsonae, flavobacterium breve, flexibacter sp. and saprospira grandis, organisms allotted to the phylum 'bacteroides-cytophaga-flavobacterium' and relatives as determined by 16s rrna analyses. by using a cluste ...19901693658
time course of changes in heart rate and sympathetic tone after coronary artery ligation in rats.the purpose of this study was to investigate the changes of heart rate (hr) and sympathetic tone occurring after myocardial infarction. male rats (200-250 g) were submitted to ligation of the anterior branches of the left coronary artery to produce infarction (inf, n = 26) or to sham surgery (so, n = 24). groups of animals (n = 6-8) were studied 1,3,7 and 15 days later. a group not submitted to surgical procedures was used as control (c,n = 6). blood pressure and hr were measured in conscious un ...19911797278
time course of changes in heart rate and sympathetic tone after coronary artery ligation in rats.the purpose of this study was to investigate the changes of heart rate (hr) and sympathetic tone occurring after myocardial infarction. male rats (200-250 g) were submitted to ligation of the anterior branches of the left coronary artery to produce infarction (inf, n = 26) or to sham surgery (so, n = 24). groups of animals (n = 6-8) were studied 1,3,7 and 15 days later. a group not submitted to surgical procedures was used as control (c,n = 6). blood pressure and hr were measured in conscious un ...19911797278
lipopolysaccharidelike immunological properties of cell wall glycoproteins isolated from cytophaga johnsonae.glycoproteins (gp) previously shown to be involved in the gliding motility of cytophaga johnsonae were examined for biological activities characteristic of lipopolysaccharide (lps). these integral membrane proteins activated 70z/3 pre-b cells to synthesize immunoglobulin m, induced b cells to synthesize non-antigen-specific polyclonal immunoglobulin, induced macrophages to produce tumor necrosis factor, and modulated the antibody response to type iii pneumococcal polysaccharide in the absence of ...19911855983
competition between strains of escherichia coli with and without plasmid rp4 during chemostat growth.escherichia coli strains j53(nal) and j53(rp4) were grown together in glucose-limited continuous cultures. based on the measured growth kinetic constants of the two strains, take-over of the cultures by j53(rp4) was predicted. however, in practice, an initial period of predominance by j53(rp4) was always followed by a prolonged period in which relative numerical proportions of the two strains oscillated widely. this period of oscillation was removed or greatly reduced when the difference between ...19911913355
functional and electrophysiological effects of oxidant stress on isolated ventricular muscle: a role for oscillatory calcium release from sarcoplasmic reticulum in arrhythmogenesis?the aim was to investigate the cellular basis of oxidant stress induced arrhythmias by studying the influence of oxidant stress on the contractile and electrophysiological function of isolated cardiac muscle.19911913755
purification and cdna sequencing of an oleate-selective acyl-acp:sn-glycerol-3-phosphate acyltransferase from pea chloroplasts.the soluble acyl-acp:sn-glycerol-3-phosphate acyltransferase from chloroplasts of chilling-sensitive and -resistant plants differ in their fatty acid selectivity. enzymes from resistant plants discriminate against non-fluid palmitic acid and select oleic acid whereas the acyltransferase from sensitive plants accepts both fatty acids. to use this difference for improving plant chilling resistance by biotechnology the gene for an oleate-selective enzyme is required. therefore, the oleate-selective ...19911932680
major salivary gland lymphoepithelial lesions and the acquired immunodeficiency syndrome.presently, there is no consensus regarding the most appropriate diagnostic and therapeutic approach to patients with human immunodeficiency virus (hiv)-associated lymphoepithelial lesions of the major salivary glands. a retrospective review of 60 consecutive patients with lymphoepithelial lesions is presented. thirty-eight cases were associated with hiv infection. lesions associated with hiv infection were usually bilateral, multiple, cystic, and associated with lymphadenopathy. in contrast, in ...19911951883
efficacy and safety of recombinant human erythropoietin to prevent the anaemias of prematurity. european randomized multicenter trial. 19912040194
[denture stomatitis. its relation to candida albicans].one of the most usual complications of the patients that carry a prosthesis is one called stomatitis denture. its etiology is not clear enough. in this work we study ninety people, sixty of them wear prosthesis. we take samples of different places in the mouth to plate and class c. albicans. we look for the relationship of results with the clinical parameters of the patient.19902203365
[resemblance of magnesium and zinc distribution in soft tissues and bone of rats fed unbalanced mineral diets in situ].although the role of zinc (zn) and magnesium (mg) in situ may be of a critical importance in replication, transcription and repair of dna, to our knowledge, the relationship between mg and zn has been described. this study, therefore, was designed experimentally to investigate the relationship between mg and zn in soft tissues and bone of rats after fortification of unbalanced mineral diets, and minerals and metals in soft tissues and bone were determined by inductively coupled plasma emission s ...19902223259
[resemblance of magnesium and zinc distribution in soft tissues and bone of rats fed unbalanced mineral diets in situ].although the role of zinc (zn) and magnesium (mg) in situ may be of a critical importance in replication, transcription and repair of dna, to our knowledge, the relationship between mg and zn has been described. this study, therefore, was designed experimentally to investigate the relationship between mg and zn in soft tissues and bone of rats after fortification of unbalanced mineral diets, and minerals and metals in soft tissues and bone were determined by inductively coupled plasma emission s ...19902223259
experimental studies on some effects of prostaglandin e2 in acute renal failure.acute renal failure has been produced in rats with oral administration of uranyl nitrate 40 mg/kg of body weight once, and histopathological changes in the kidneys with blood biochemical values were studied by sacrificing them after 36 hours of feeding. prostaglandin e2 in 100 micrograms/kg of body weight was orally administered to another group of rats along with uranyl nitrate 40 mg/kg of body weight once and sacrificed them 36 hours after feeding. it was found that prostaglandin e2 has some p ...19902362130
serological relationships between serotype-iii streptococcus sanguis and lancefield group-h streptococci.certain strains of streptococcus sanguis and group-h streptococci have been shown to have similar physiological properties and serological specificities. serological studies revealed that serotype-iii s. sanguis shared a common antigen with the so-called "british" group-h streptococci, but not with the "american" group-h streptococci. serotype-iii antigen was extracted in cold 5% trichloroacetic acid from isolated cell walls of s. sanguis atcc 10558, and purified chromatographically. the purifie ...19872438413
[current bacteriologic status and therapeutic results in acute otitis media in children aged over 3 months].355 children, 3 months to 15 years old, presenting with acute otitis media, underwent a bacteriological study of otitis exsudate. from november 1985 to september 1987, 162 myringotomy and 149 acute otitis media spontaneously discharging were studied in paris. from october 1981 to september 1987, 62 myringotomy and 46 spontaneously discharging acute otitis were studied in valognes. there was none pathogenic bacterium in respectively 20 and 14% of the samples. two or more pathogenic bacterium were ...19892491709
cysteine is not an obligatory intermediate in the biosynthesis of cysteate by cytophaga johnsonae.a cysteine auxotroph of cytophaga johnsonae was able to incorporate sulfur from sulfate into cysteate, and thus into sulfonolipid, in the absence of cysteine synthesis. this indicates that cysteine is not an obligatory intermediate of the cysteate biosynthetic pathway even though cysteine sulfur can be utilized for cysteate synthesis.19892541704
cryptococcus neoformans var. neoformans and munia birds.cr. neoformans var. neoformans has been persistently demonstrated in the saprobic environment of caged munia birds at a local zoological garden. the pathogen was also cultured from the beaks, feathers and legs of these birds. it is suggested that the role of munia birds should be further investigated to understand the ecology of this important human pathogen.19892668756
left atrial hypertension causes pleural effusion formation in unanesthetized sheep.we studied the effect of left atrial pressure (lap) elevation on the formation of pleural effusion in unanesthetized sheep. we prepared the animals by placing catheters in the left atrium, pulmonary artery, femoral artery, and vein. we also placed a balloon catheter in the left atrium. after a recovery period of at least 1 wk, we measured lap, pulmonary artery pressure (pap), systemic arterial pressure, systemic venous pressure, cardiac output, plasma protein concentration, and plasma colloid os ...19892764149
twenty-year comparison of the human allograft and porcine xenograft.this study compares a retrospective consecutive series of human allografts and concurrent porcine xenografts implanted over a 10-year interval. there were 571 allograft valves and 1,351 xenograft valves implanted in the aortic and mitral position with mean follow-up of 12.8 years for the allografts and 6.2 years for the xenografts. this study compares the incidence of structural deterioration over long-term follow-up. we found that there was no significant difference between four manufacturers o ...19892774760
specificity and cellular distribution of human polymorphonuclear leucocyte receptors for leukotriene c4.human polymorphonuclear (pmn) leucocytes bind synthetic [3h]-labelled leukotriene c4 ([3h]ltc4) with rapid saturation and reversibility of approximately 90%, by a 700-fold higher concentration of non-radioactive ltc4. [3h]ltc4 is recognized specifically by 10,778 +/- 6260 (mean +/- sd) sites per pmn leucocyte that exhibit a kd of 34.3 +/- 1.7 nm. the specificity of the ltc4 receptors is supported by the competitive inhibition of binding of [3h]ltc4 by ltc4, ltc4-sulphone, ltd4, and lte4 with rel ...19872820869
specificity and cellular distribution of human polymorphonuclear leucocyte receptors for leukotriene c4.human polymorphonuclear (pmn) leucocytes bind synthetic [3h]-labelled leukotriene c4 ([3h]ltc4) with rapid saturation and reversibility of approximately 90%, by a 700-fold higher concentration of non-radioactive ltc4. [3h]ltc4 is recognized specifically by 10,778 +/- 6260 (mean +/- sd) sites per pmn leucocyte that exhibit a kd of 34.3 +/- 1.7 nm. the specificity of the ltc4 receptors is supported by the competitive inhibition of binding of [3h]ltc4 by ltc4, ltc4-sulphone, ltd4, and lte4 with rel ...19872820869
comparison of the phosphatases of lysobacter enzymogenes with those of related bacteria.lysobacter enzymogenes atcc 29487 (uasm 495) produces an outer-membrane-associated phosphatase and an excreted phosphatase. the cell-associated enzyme was compared to phosphatases of nine other gram-negative gliding bacteria and to that of escherichia coli. the other three species of the genus lysobacter also produce a particulate, cell-associated phosphatase. antiserum prepared against the phosphatase from the outer membrane of l. enzymogenes effectively precipitated the phosphatases of two oth ...19872833562
[therapy of aids].therapeutic strategies in the management of aids should aim at an inhibition of the replication and spread of hiv; the reconstitution of immune functions; an effective chemotherapy of opportunistic infections and tumors; and the prevention of new opportunistic infections. at present, these goals can be only partially achieved and where beneficial therapeutic results are obtained they are only transient in nature. antiretroviral therapy and chemotherapy of infections have, however, resulted in so ...19882849247
safety and efficacy of a recombinant dna plasmodium falciparum sporozoite vaccine.a recombinant dna plasmodium falciparum sporozoite vaccine produced in escherichia coli (fsv-1) was tested in doses of 10 micrograms to 800 micrograms protein in fifteen volunteers. no serious adverse reactions occurred. antibodies that reacted with p falciparum sporozoite antigens by enzyme-linked immunoassay developed in twelve of the volunteers. the highest antibody titres induced were similar to those resulting from lifelong natural exposure to sporozoite-infected mosquitoes. postimmunizatio ...19872884410
increase of ornithine amino lipid content in a sulfonolipid-deficient mutant of cytophaga johnsonae.the gram-negative gliding bacterium cytophaga johnsonae contains not only large quantities of unusual sulfonolipids but also, as we report here, a second class of unusual lipids. these lipids were detected and quantified by two-dimensional thin-layer chromatography of lipids from cells grown in the presence of [14c]acetate and shown by chemical studies to be alpha-n-(3-fatty acyloxy fatty acyl)ornithines. like the sulfonolipids, these ornithine lipids were localized in the outer membrane (wherea ...19892914878
effect of mononuclear cell factors on calcium transport in sarcoplasmic reticulum vesicles.the effect of supernatants from cultures of mitogen-stimulated human mononuclear cells on calcium transport by sarcoplasmic reticulum was examined. calcium transport was assayed by measuring the time course of calcium accumulation by sarcoplasmic reticulum incubated with supernatants from stimulated mononuclear cells was 20% less than that by vesicles exposed to control supernatants (p less than 0.001). in contrast, no difference in calcium-dependent atpase activity was noted between vesicles in ...19862946307
hyperthyroidism selectively increases oxidative metabolism of slow-oxidative motor units.the effects of thyroid hormone on the nadh-tetrazolium reductase activity (oxidative metabolism marker) of soleus (slow-oxidative) and tensor fascia lata (fast-glycolytic) motoneurons were determined and compared with changes in a variety of enzyme activities in the corresponding muscle fibers. histochemical assays have demonstrated a selective and qualitative conversion in muscle fiber atpase and quantitative increases of nadh-tetrazolium reductase (oxidative) and mitochondrial alpha-glyceropho ...19872953623
[intraabdominal cystic anaplastic seminoma in a 67-year-old man]. 19872893480
gamma-glutamyl transpeptidase activity in ascaris suum.using histochemical techniques gamma-glutamyl transpeptidase activity has been localized mainly in the cuticle-hypodermis in ascaris suum. the specific activity of gamma-glutamyl transpeptidase was 3.87 +/- 0.49 mumol h-1 (g tissue)-1 in cuticle-hypodermis as compared to gastrointestinal tract, where it was 0.07 +/- 0.02 mumol h-1 (g tissue)-1, and muscle and reproductive tract where it was less than 0.001 mumol h-1 (g tissue)-1. when cuticle sections were incubated with labelled glutamine a lar ...19862876379
[ecology and epidemiology of transmissible viruses of sandflies in italy]. 19863019208
analysis of pectate lyases produced by soft rot bacteria associated with spoilage of vegetables.isoelectric focusing (ief) profiles of pectate lyases (pls) produced by five different groups of soft rot bacteria were analyzed by using the combined techniques of thin-layer polyacrylamide gel ief and agarose-pectate overlay activity staining. four strains of soft rot erwinia spp. produced three or more pl isozymes. all of eight pseudomonas viridiflava strains examined produced one single pl with a pi of 9.7. all 10 of pseudomonas fluorescens strains produced two pls; the major one had a pi of ...19892764574
accurate and efficient rna polymerase iii transcription in a cell-free extract prepared from ascaris suum embryos.high-speed supernatant (s100) extracts derived from homogenized ascaris suum embryos efficiently transcribe added rna polymerase iii templates including cloned 5s rrna genes of the filarial parasite brugia malayi. several criteria, including two-dimensional rnase t1 oligonucleotide fingerprint analysis, indicate that in vitro transcription is accurately initiated and terminated.19892747746
[changes in the properties of brain opiate receptors during the development of morphine tolerance in rats].the effects of prolonged administration of morphine on the properties of opiate receptors of rat brain were studied. for this purpose the isotherms of binding of labeled mu-, delta-, and chi-ligands--morphine, d-ala2, d-leu5-enkephalin and ethylketocyclazocine--with brain membrane preparations of morphine-tolerant rats as well as those of control animals were analyzed. for quantitative determination of dissociation constants of the ligand-receptor complexes (k) and receptor concentrations ([q]), ...19863021240
[changes in the properties of brain opiate receptors during the development of morphine tolerance in rats].the effects of prolonged administration of morphine on the properties of opiate receptors of rat brain were studied. for this purpose the isotherms of binding of labeled mu-, delta-, and chi-ligands--morphine, d-ala2, d-leu5-enkephalin and ethylketocyclazocine--with brain membrane preparations of morphine-tolerant rats as well as those of control animals were analyzed. for quantitative determination of dissociation constants of the ligand-receptor complexes (k) and receptor concentrations ([q]), ...19863021240
aldosterone production after short-term culture of rat adrenal capsule: responsiveness to angiotensin ii, potassium and acth.in vitro studies of aldosterone production have traditionally used freshly isolated adrenal glomerulosa tissue. in the present study we examined the effects of short-term culture of rat adrenal capsule on its secretory capacity by measuring both basal and stimulated aldosterone production. capsules were maintained in culture for 24 h, and then responses to administered angiotensin ii (1 x 10(-7) m), potassium (an increase of 2mm) and acth (1 x 10(-8) m) were determined during perifusion. results ...19873035282
sinusoidal structure of the liver.following a review of current information on the structural and functional characteristics of liver tissue with view to explaining the heterogeneity of the structure of the sinusoids within each microcirculatory unit, we analyze the zone-specific properties of hepatic parenchymal and non-parenchymal cells. however, in addition to zonal differentiation of hepatocytes with respect to their metabolic activities, we also discuss the appreciable zonal variations along the sinusoidal pathway in the ca ...19883067270
monoclonal antibodies reacting with the exopolysaccharide xanthan from xanthomonas campestris.we have prepared murine hybridomas secreting monoclonal antibodies against the exopopolysaccharide xanthan from xanthomonas campestris pv. campestris 646 after fusing nso myeloma cells and spleen cells from balb/c mice immunized with xanthan. four hybridomas, secreting antibodies designated a6 (igm kappa), b3 (igm kappa), d1 (igm kappa), and d3 (igg2a kappa), were selected for further studies. all antibodies reacted with a range of different xanthans. competition studies using variants of the ex ...19892621443
sulphadiazine resistant strains of neisseria meningitidis during an outbreak of meningococcal meningitis. 19873111997
temperate phages and bacteriocins of the gliding bacterium cytophaga johnsonae.a collection of 30 independently isolated strains of cytophaga johnsonae was screened for the presence of temperate bacteriophages. two strains were found to harbour phages. the newly isolated phages differ in several respects from the 43 previously isolated phages for c. johnsonae. both phages are polyhedral, approximately 60 nm in diameter, and have no apparent tail structure. they are chloroform sensitive, and plaque formation is inhibited by agar. both are capable of establishing a stable as ...19883171539
monoclonal antibodies reacting with the exopolysaccharide xanthan from xanthomonas campestris.we have prepared murine hybridomas secreting monoclonal antibodies against the exopopolysaccharide xanthan from xanthomonas campestris pv. campestris 646 after fusing nso myeloma cells and spleen cells from balb/c mice immunized with xanthan. four hybridomas, secreting antibodies designated a6 (igm kappa), b3 (igm kappa), d1 (igm kappa), and d3 (igg2a kappa), were selected for further studies. all antibodies reacted with a range of different xanthans. competition studies using variants of the ex ...19892621443
cytotoxicity and effect on mutagenicity of buffers in a microsuspension assay.we have examined the effects of three buffers--vogel-bonner minimal e (vbm) and 0.15 and 0.015 m sodium phosphate, ph 7.4--and two concentrations of overnight cells (5x and 10x) on the mutagenicity of two pure compounds and two complex mixtures in strain ta98 using a modification of a microsuspension assay developed by kado et al. (mutation research 121:25-32, 1983). the assay was performed by adding 50 microliters of cell concentrate of an overnight culture of ta98 resuspended in the appropriat ...19892575290
fluorocarbon (fc-43) in augmentation of tissue oxygen and its experimental use for flap survival.the tissue po2 level in the skin of rats injected with fluorocarbon (fc-43) was measured by the microelectrode method and by a transcutaneous monitor. it was found that the tissue po2 in the animals injected with fc-43 was two to three times higher than that of the control group. neither the administration of fc-43 nor the inhalation of systemic oxygen improved flap survival in the rats.19863273019
ornithine cyclodeaminase from ti plasmid c58: dna sequence, enzyme properties and regulation of activity by arginine.nopaline, an abundant opine in plant cells transformed with nopaline-type ti plasmids, is catabolized in agrobacterium by three ti-plasmid-coded steps via arginine and ornithine to proline. the last enzyme, ornithine cyclodeaminase (ocd), converts ornithine directly into proline with release of ammonia. we describe the dna sequence of the ocd gene from ti plasmid c58, antiserum against an ocd fusion protein overexpressed in escherichia coli, induction and identification of the gene product in ag ...19883281832
characterization of the copper-thiolate cluster in yeast metallothionein and two truncated mutants.cu-metallothionein was purified from saccharomyces cerevisiae harboring plasmids containing mutated cup1 metallothionein genes resulting in deletions at the carboxy-terminal end of the polypeptide. the truncated polypeptides are recovered as polypeptides of 35 and 48 residues in length. the cu-s cluster in the wild-type metallothionein and the two truncates were characterized. the truncated proteins, designated t35 and t48, contain 4 and 2 fewer cysteinyl residues, respectively, compared to the ...19883283130
growth of pure cultures of retinal pigment epithelial cells using chorioretinal biopsies from the pig. 19883289957
clearance of chlamydial elementary bodies from the conjunctival sac.the rate of disappearance of inactivated chlamydia trachomatis elementary body (eb) preparations from the conjunctival sac was studied in monkeys. direct fluorescent antibody (dfa) cytology showed that the majority of eb had been cleared from the eye within 24 hr of the inoculation of 1 x 10(6) inactivated eb, although small numbers of eb could be detected for up to 144 hr. the rate of clearance in normal and ocular immune animals did not differ, and formalin-killed and uv-inactivated ebs disapp ...19873298129
[mechanisms of thrombin-induced plasminogen activator release].the protein kinase c inhibitor h7 (10(-5) mol/l) is able to inhibit the thrombin-induced t-pa release in the isolated perfused pig ear. the thrombin-induced t-pa release can be blocked by increasing the intracellular c-amp via either the activation of adenylate cyclase by means of forskolin, or the inhibition of the phosphodiesterase by means of motapizone or milrinone. protein kinase c is assumed to be involved in the process of thrombin-induced t-pa release.19892483708
satellite cells in the regenerated and regrafted skeletal muscles of rats.soleus (sol) muscles were grafted into extensor digitorum longus (edl) muscle beds (edl-first-graft). sixty days later, some mature edl-first-grafts were regrafted into their own beds (edl-second-grafts). fully regenerated muscle fibers and satellite cells were observed in both types of mature grafts. the ratios of satellite cell nuclei per total nuclei (myonuclei and satellite cell nuclei) were 4.81 +/- 0.47% for edl-2nd graft, 4.26 +/- 0.51% for edl-1st-graft, 4.30 +/- 0.33% for control sol, a ...19883396655
use of nonmotile mutants to identify a set of membrane proteins related to gliding motility in cytophaga johnsonae.nonmotile mutants of the gliding bacterium cytophaga johnsonae were examined to identify proteins that might be involved in gliding motility. wild-type and mutant cell proteins were solubilized and fractionated by using triton x-114, and the proteins that partitioned into the aqueous phase or the detergent phase were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis for proteins that differed between wild-type and mutant cells. seventeen proteins, ranging in size from 16 to 1 ...19902345138
comparative biodistributions and rates of catabolism of radiolabelled anti-cea monoclonal antibody and control immunoglobulin in nude mice with human tumour xenografts showing specific antibody localization.the rate of catabolism of a radiolabelled monoclonal anti-cea antibody has been compared with that of control igg1 in control nude mice and in mice with cea producing xenografts and antigen negative xenografts. the rate of catabolism of antibody, but not of igg, was increased 3-4 fold in mice with xenografts localizing the antibody, but not in mice with antigen negative tumours. there was no evidence of immune complex formation and/or clearance of antibody from the serum of xenografted mice and ...19873478194
outer membrane polysaccharide deficiency in two nongliding mutants of cytophaga johnsonae.phenol-extractable polysaccharides firmly associated with the outer membrane of the gliding bacterium cytophaga johnsonae could be resolved by gel filtration in sodium dodecyl sulfate (sds) or by sds-polyacrylamide gel electrophoresis into a high-molecular-weight (h) fraction (excluded by sephadex g-200) and a low-molecular-weight (l) fraction. fraction l was rich in components typical of lipid a and the core region of lipopolysaccharide (p, 3-hydroxy fatty acids, and 2-keto-3-deoxyoctonate) and ...19902307648
defects in gliding motility in mutants of cytophaga johnsonae lacking a high-molecular-weight cell surface polysaccharide.we previously observed (w. godchaux, l. gorski, and e.r. leadbetter, j. bacteriol. 172:1250-1255, 1990) that two mutants (strains 21 and ns-1) of the gliding bacterium cytophaga johnsonae that were totally deficient in motility-dependent colony spreading, movement of rafts (groups) of cells as observed with a microscope, and movement of polystyrene-latex spheres that attached to the cell surface (observed in wet mounts) were also deficient in a high-molecular-weight cell surface polysaccharide ( ...19911938956
temporal sequence of the recovery of traits during phenotypic curing of a cytophaga johnsonae motility mutant.the lack of cell translocation and the resulting formation of nonspreading colonies of mutants of the gram-negative gliding bacterium cytophaga johnsonae have been correlated with the loss of cell surface features of the organism. these cell surface traits include the ability to move polystyrene-latex beads over the cell surface and the ability to be infected by bacteriophages that infect the parent strain. in order to assess whether these traits reflect structures or functions that actually pla ...19911938948
phospholipids and a novel glycine-containing lipoamino acid in cytophaga johnsonae stanier strain c21.two-dimensional thin-layer chromatography revealed that cytophaga johnsonae contains at least 10 kinds of lipid, 2 of which are phospholipids, namely, phosphatidylethanolamine and phosphatidylcholine. one of the remaining lipids is a novel lipid that contains an amino acid. the structure of this unusual lipid (lipoamino acid) was resolved by chemical and physicochemical methods. the fatty acyl moiety of this lipid was diverse. the structure of the major molecular species of the lipid was determi ...19911885525
effects of sodium selenite and caffeine on mutagenesis induced by n-methyl-n-nitrosourea, n-methyl-n'-nitro-n-nitrosoguanidine and aflatoxin b1 in s. typhimurium.pre-treatment, co-treatment, and post-treatment procedures were comparatively used in order to assess the modulation of mutagenicity in s. typhimurium his- strains. pre-treatment of bacteria with sodium selenite had no effect on sodium azide mutagenicity. irrespective of the procedure used neither selenite nor caffeine had any influence on the s9-mediated mutagenicity of aflatoxin b1. in contrast, the mutagenicity of n-methyl-n-nitrosourea (mnu) and n-methyl-n'-nitro-n- nitrosoguanidine (mnng) w ...19921383715
partitioning of upper airway resistance in friesian and double-muscled calves.respiratory resistance in calves was partitioned in two components: upper airway resistance and pulmonary resistance. the former one was divided into naso-pharyngeal and laryngeal resistance. a comparison between seven healthy unsedated double-muscled calves of the belgian white and blue breed (bwb) and five healthy unsedated friesian (f) calves was performed. respiratory resistance was significantly greater in the bwb calves, due to the significantly higher values of the naso-pharyngeal and the ...19873659600
dna structure, mutations, and human genetic disease.the etiology of fragile x syndrome, myotonic dystrophy and kennedy's disease has been attributed to the massive expansion of triplet repeat dna sequences. this review details the relationships between the structural diversity of dna, its secondary structure or dna-directed mutagenesis, and the expansion of triplet repeats.19921369117
[study of local shiftings of the brain stem in increased intracranial pressure by a supratentorial balloon].the study of the mechanism of pressure transmission is important for the treatment of rapid deterioration of a patient's condition with increased intracranial pressure (icp) from a space taking lesion in neurosurgical practice. the aim of this experiment is to clarify the mechanism of pressure transmission during supratentorial balloon inflation, by continuously monitoring local shiftings of the brain using strain gauge sensors. mongrel dogs and macaca fuscatas were fixed in stereotaxic head fra ...19863718782
conducting airway gas exchange: diffusion-related differences in inert gas elimination.we studied co2 and inert gas elimination in the isolated in situ trachea as a model of conducting airway gas exchange. six inert gases with various solubilities and molecular weights (mw) were infused into the left atria of six pentobarbital-anesthetized dogs (group 1). the unidirectionally ventilated trachea behaved as a high ventilation-perfusion unit (ratio = 60) with no appreciable dead space. excretion of higher-mw gases appeared to be depressed, suggesting a mw dependence to inert gas exch ...19921317375
properties of cytophaga johnsonae strains causing spoilage of fresh produce at food markets.two strains of gliding, orange-pigmented bacteria, isolated from fresh bell pepper and watermelon, respectively, showing soft-rot lesions, were identified as cytophaga johnsonae. they differed from seven type strains of c. johnsonae deposited at the american type culture collection (atcc) in some properties, such as the ability to utilize glucose, xylose, trehalose, rhamnose, and sucrose. spherical bodies resembling microcysts of sporocytophaga sp. in addition to short rods and long filaments we ...19863789718
separating the effects of response rate and reinforcement frequency in the rate-dependent effects of amphetamine and scopolamine on the schedule-controlled performance of rats and pigeons.rats and pigeons responded under three-component multiple schedules of food reinforcement. each component was associated with a separate stimulus condition and a schedule that arranged food delivery intermittently for appropriately spaced responding. in the experiment with pigeons, three response rates were maintained with equal reinforcement frequency in the three components. with rats, the lowest response rate was associated with a higher reinforcement frequency. the effects of d-amphetamine i ...19751151762
distribution of multicopy single-stranded dna among myxobacteria and related species.multicopy single-stranded dna (msdna) is a short single-stranded linear dna originally discovered in myxococcus xanthus and subsequently found in stigmatella aurantiaca. it exists at an estimated 500 to 700 copies per chromosome (t. yee, t. furuichi, s. inouye, and m. inouye, cell 38:203-209, 1984). we found msdna in other myxobacteria, including myxococcus coralloides, cystobacter violaceus, cystobacter ferrugineus (cbfe17), nannocystis exedens, and nine independently isolated strains of m. xan ...19853932332
decontamination of cell lines by selective elimination of mycoplasmas. 19863964004
decontamination of cell lines by selective elimination of mycoplasmas. 19863964004
monkey dander asthma.we recently cared for two patients who experienced acute asthma with exposure to dander of the cotton top tamarin, a species of new world monkey. both patients had serologic evidence for an ige antibody to monkey dander as determined by rast and a positive immediate skin test response to an extract prepared from monkey dander. furthermore, by rast we were able to determine that cotton top tamarin urine and newborn cotton top tamarin dander had antigens that reacted with ige in the serum of the a ...19853968337
metastatic nontraumatic clostridium septicum osteomyelitis.nontraumatic clostridial infections are rare, but need to be diagnosed and treated early or high morbidity and mortality rates result. we believe that this is the first reported case of metastatic nontraumatic clostridium septicum osteomyelitis. early treatment with surgical debridement and parenteral antibiotics without hyperbaric oxygen was used. an associated occult rectal malignancy was discovered and treated.19853980945
extracellular distribution of radiolabel obscures specific binding of diethylstilbestrol in mouse skeletal muscle.the extracellular distribution of 3h-diethylstilbestrol (3h-des) in mouse skeletal muscle was assessed following intraperitoneal injection. total muscle extracellular space was measured with 14c-inulin, and the vascular space with 125i-albumin. a significant difference in the distribution of native 3h-des and its metabolites in muscle and blood was found. this could only be explained if these compounds distributed with the albumin space and not the inulin space.19853992019
[toxicological characteristics of halomethanes--dichlorobromomethane and dibromochloromethane formed during water chlorination]. 19854007549
definitive chromosomal location of the h-2 complex by in situ hybridization to pachytene chromosomes.chromosome 17 of the mouse carries the h-2 complex and the t/t complex. an understanding of the organization of this region and an accurate genetic map of chromosome 17 would be of great value for both immunologists and developmental biologists. until now the only maps available have been derived solely from recombinational studies using several translocations, an inherently inaccurate method. we have found the definitive location of the h-2 complex by the use of in situ hybridization. our resul ...19854018853
investigations of the pigments from cytophaga johnsonae cy jl. new flexirubin-type pigments.besides carotenoids a complex of flexirubin-type pigments was isolated from the gliding bacterium cytophaga johnsonae cy jl and separated into 6 components, which partly containe chlorine. in spite of the fact that these components still consist of pigment mixtures, the gross structures of 18 new flexirubin-type pigments could be deduced by spectroscopic and chemical investigations. the results open insights into biosynthesis and structural variety of the flexirubins, the novel non-isoprenoid pi ...1978697500
a numerical taxonomic study of flavobacterium-cytophaga strains from dairy sources.phenotypic data on 203 gram-negative non-fermentative bacteria of the flavobacterium-cytophaga group isolated from milk and butter were analyzed by numerical taxonomic techniques. twenty reference strains including species of flavobacterium, cytophaga and strains of pseudomonas paucimobilis were included in the study. using the matching coefficient of sokal & michener with antibiotic susceptibility data included, 189 isolates were recovered in nine clusters. six of these clusters were linked at ...19854066550
cortical temperature during paradoxical sleep in the monkey. 19684174781
oxygen consumption of the germinal epithelium in psoriatic human skin as measured by the cartesian diver micro-gasometer. 19684185292
implantation of the rat embryo: glycogen and alkaline phosphatases. 19664288674
implantation of the rat embryo: glycogen and alkaline phosphatases. 19664288674
[the effect of various gamma aminobutyric acid doses on the total and individual phospholipid levels in whole blood entering and leaving the brain]. 19684300822
[similarity of changes in liver cell enzyme activity due to the immediate effects of carcinogens and after malignization]. 19704324048
incorporation of n-acetylglucosamine into the core region of the cell wall lipopolysaccharide of escherichia coli. 1979374407
Displaying items 1 - 100 of 468