characterization of the neoagarotetra-ase and neoagarobiase of cytophaga flevensis. | the degradation of neoagarotetraose and neoagarobiose by cytophaga flevensis was investigated. the organism possesses an enzyme that hydrolyzes the tetramer by cleavage of its central beta-galactosidic linkage. the product of this reaction, neoagarobiose, is further hydrolyzed enzymatically to d-galactose and 3,6-anhydro-l-galactose. both enzyme activities were localized in the cytoplasm. attempts were made to partially purify the respective enzymes and although a 30-40 fold-purification was ach ... | 1976 | 8001 |
the regulation of agarase production by resting cells of cytophaga flevenis. | the regulation of the synthesis of extracellular agarase by cytophaga flevensis was studied in resting-cell suspensions. enzyme synthesis was strictly dependent on the presence of a suitable inducer. enzyme production was maximal at 20 c in phosphate buffer ph 6.9 in the presence of 1.3 mm calcium chloride, 0.03% casamino acids and inducer. enzyme production was virtually the same at 15 and 20 c, reduced to 50% at 25 c and was not detectable at 30 c. it was highly stimulated by the presence of 0 ... | 1976 | 10834 |
production and characterization of the agarase of cytoplaga flevensis. | cytophaga flevensis produced an inducible agarase which was extracellular under most conditions tested. the effect of cultural conditions on the production of enzyme was studied in batch and continuous culture. in batch culture, production was optimal when cytophaga flevensis was incubated at 20 c in a mineral medium with agar as the sole carbon source and ammonium nitrate as the nitrogen source at an initial ph of 6.6-7.0. the enzyme appeared to be subject to catabolite repression, since its sy ... | 1975 | 1083206 |
isolation and characterization of cytophaga flevensis sp. nov., a new agarolytic flexibacterium. | | 1974 | 4604642 |
flavobacterium gelidilacus sp. nov., isolated from microbial mats in antarctic lakes. | twenty-two isolates from microbial mats in eastern antarctic lakes showed similar fatty acid compositions and were investigated further using a polyphasic taxonomic approach. repetitive extragenic palindromic dna-pcr fingerprinting of the 22 strains revealed three groups, and dna-dna hybridizations between representatives showed more than 87 % dna-dna reassociation with each other. 16s rrna gene sequence analysis placed two representative strains, lmg 21477(t) and lmg 21619, within the genus fla ... | 2003 | 13130001 |
dechloromonas denitrificans sp. nov., flavobacterium denitrificans sp. nov., paenibacillus anaericanus sp. nov. and paenibacillus terrae strain mh72, n2o-producing bacteria isolated from the gut of the earthworm aporrectodea caliginosa. | earthworms emit nitrous oxide (n(2)o) via the activity of bacteria in their gut. four n(2)o-producing facultative aerobes, ed1(t), ed5(t), mh21(t) and mh72, were isolated from the gut of the earthworm aporrectodea caliginosa. the isolates produced n(2)o under conditions that simulated the microenvironment of the earthworm gut. ed1(t) and ed5(t) were gram-negative, motile rods that carried out complete denitrification (i.e. the reduction of nitrate to n(2)) and contained membranous c-type cytochr ... | 2005 | 15879265 |
flavobacterium weaverense sp. nov. and flavobacterium segetis sp. nov., novel psychrophiles isolated from the antarctic. | two psychrophilic flavobacteria, designated at1042(t) and at1048(t), were isolated from terrestrial samples from the antarctic. results of 16s rrna gene sequence analyses indicated a close relationship of these isolates to flavobacterium flevense (96.9 % similarity for strain at1042(t)) and flavobacterium psychrolimnae (97.0 % for strain at1048(t)). cells were non-motile and non-gliding. flexirubin-type pigments were absent. both isolates were psychrophilic, with an optimum and maximum growth te ... | 2006 | 16738098 |
the effect of isotonic amino acid infusion on nitrogen balance in the diabetic. | | 1998 | 106748 |
flavobacterium oryzae sp. nov., isolated from a flooded rice field, and emended descriptions of flavobacterium flevense, flavobacterium yonginense and flavobacterium myungsuense. | a bacterial strain, designated jyi-05(t), was isolated from a flooded rice field and characterized using a polyphasic taxonomic approach. cells of strain jyi-05(t) were gram-stain-negative, strictly aerobic, bright-yellow-pigmented rods that were motile by gliding. growth occurred at 10-30 °c (optimum 30 °c), at ph 6.5-7.5 (optimum ph 7.0) and with 0-2% (w/v) nacl (optimum 1%, w/v, nacl). phylogenetic analyses based on 16s rrna gene sequences showed that strain jyi-05(t) belonged to the genus fl ... | 2014 | 25106925 |