| cytotoxicity of oxidation derivatives of cholesterol on cultured aortic smooth muscle cells and their effect on cholesterol biosynthesis. | aortic smooth muscle cell death is an important initial lesion of atherosclerosis. a number of autooxidation products of cholesterol which has been recognized recently has the capability of inducing rabbits' aortic smooth cell death in vitro. twelve oxidation derivatives of cholesterol, available commercially, were dissolved in small amounts of ethanol, then added to the culture medium at levels not exceeding 0.8%. the medium contained 10% fetal calf's serum which served as an in situ vehicle fo ... | 1979 | 34998 |
| [effect of human antigens on the biological properties of the causative agents of typhoid fever and paratyphoid b]. | | 1979 | 91281 |
| condensation--inhibition by 33258-hoechst of centromeric heterochromatin in prematurely condensed mouse chromosomes. | | 1979 | 91529 |
| [infection as indicator of finding complex environmental noxious agents within a short time (author's transl)]. | we do not know whether complexes of chemical agents produce an accumulation of their respective noxious effects or whether these effects weaken each other and we have no exact methods to quantify these effects. knowledge of such methods would be important for investigation of environmental health. in the course of researches how to influence infectious diseases by altering natural resistance we have found that it might be possible to use an indicator method: animals exposed to such a light envir ... | 1979 | 92117 |
| an investigation of the optimal conditions for the in vivo production of immunologically sensitised rat mast cells. | experiments were designed to develop an optimal method for inducing in vivo production of sensitised peritoneal mast cells. rats of different strains were sensitised with whole egg-white and killed at suitable intervals to harvest the peritoneal mast cells. release of histamine was induced in vitro by both whole egg-white and its major protein constituents, and assayed by a standard spectrofluorometric method. wistar rats showed higher levels of sensitisation than black-hooded lister rats; it wa ... | 1979 | 92507 |
| partial amino acid sequences of rabbit and rat beta 2-microglobulins. | | 1979 | 93696 |
| purification of antibodies to influenza a virus structural proteins by affinity chromatography, and their participation in haemagglutination-inhibition, neutralization and enzyme-linked immunosorbent assay. | affinity chromatography was used to purify rabbit antibodies to common and strain-specific antigeneic determinants of haemagglutinin, to neuraminidase, and to a combination of the internal proteins of influenza a viruses. the purity of the antibodies was assessed by haemagglutination-inhibition, enzyme-linked immunosorbent assay (elisa) and competition elisa. the antibodies were examined for their participation in neutralization and haemagglutination-inhibition assays, and in elisa. elisa was fo ... | 1979 | 93960 |
| an unstable donor-recipient dna complex in transformation of bacillus subtilis. | in re-extracted dna obtained shortly after uptake of transforming dna by bacillus subtilis, increased amounts of donor dna radioactivity banding at the position of donor-recipient dna complex (drc) are observed in cscl gradients, if the cells are irradiated with high doses of uv prior to reextraction of the dna. qualitatively, the same phenomenon is observed if lysates of transforming cells are irradiated. uv-irradiation of lysates of competent cells to which single-stranded dna is added after l ... | 1978 | 106232 |
| changes in lung ultrastructure following heterologous and homologous serum albumin infusion in the treatment of hemorrhagic shock. | the object of this study was to compare the ultrastructure pulmonary effects of the infusion of homologous and heterologous serum albumin solution in the treatment of hemorrhagic shock in baboons. adult baboons subjected to hemorrhagic shock were resuscitated with either baboon serum albumin, human serum albumin, or ringer's lactate solution. the lungs were fixed in vivo with potassium pyroantimony, a solution which produces electron dense interstitial precipitation of sodium. the lungs from ani ... | 1979 | 106780 |
| office microbiology. | | 1979 | 111268 |
| use of micro-elisa for quantitating antibody to trypanosoma cruzi and trypanosoma rangeli. | of 229 residents of a panamanian village where both trypanosoma cruzi and trypanosoma rangeli are endemic, 52% had antibody to one or both species by the enzyme-linked immunosorbent assay (elisa), 26% were positive by complement fixation, 17% were positive by direct agglutination, and 32% were positive on the basis of clinical impression. although the sensitivity of elisa makes it the procedure of choice for sero-epidemiologic studies, there does appear to be some serologic cross-reactivity betw ... | 1979 | 116559 |
| surface properties and in vitro functional capacities of human t lymphocyte subsets separated by linear density gradients. | human lymphocytes derived from various central and peripheral sources were separated on linear density gradients (ldg). cells from individual density fractions were tested in parallel for: the capacity to form nonimmune rosettes with neuraminidase-treated srbc, the number of surface-associated htla, and in vitro proliferative responses to mitogenic lectins and alloantigens. heterogeneous density distribution profiles were obtained for all sources of human t cells and revealed an organ specificit ... | 1979 | 156234 |
| reversible hypothyroidism in growth hormone-deficient children treated with human growth hormone. | six children with human growth hormone (hgh) deficiency became hypothyroid during the course of their therapy with hgh. this was accompanied by a decreasing growth rate, clinical symptoms of hypothyroidism and decreased serum t4 concentrations. three of the 6 patients returned to the euthyroid state, both clinically and biochemically, with cessation of hgh therapy, and reinstitution of hgh precipitated hypothyroidism again in 2 of the three. the patients who remained hypothyroid have evidence of ... | 1975 | 165211 |
| conditional lethal mutants of vesicular stomatitis virus. | | 1975 | 169102 |
| relationship of lactate dehydrogenase specificity and growth rate to lactate metabolism by selenomonas ruminantium. | a lactate-fermenting strain of selenomonas ruminantium (hd4) and a lactatenonfermenting strain (ga192) were examined with respect to the stereoisomers of lactate formed during glucose fermentation, the stereoisomers of lactate fermented by hd4, and the characteristics of the lactate dehydrogenases of the strains. ga192 formed l-lactate and hd4 formed l-lactate and small amounts of d-lactate from glucose. hd4 fermended l- but not d-lactate. both strains contain nicotinamide adenine dinucleotide ( ... | 1975 | 174490 |
| a structural study of pig liver glyceraldehyde-3-phosphate dehydrogenase. | a substantial portion of the primary structure of pig liver glyceraldehyde-3-phosphate dehydrogenase has been investigated and the results compared with those previously reported for the pig muscle enzyme. liver and muscle glyceraldehyde-3-phosphate dehydrogenases show the same amino acid content, and the first n-terminal residues occur in the same sequence. no differences in n-terminal residues and amino acid composition have been evidenced by analysis of several tryptic peptides, which account ... | 1976 | 182238 |
| sv40 and adenovirus early functions involved in dna replication and transformation. | | 1976 | 184845 |
| cross-reactions between bordetella pertussis and twenty-eight other bacterial species. | cross-reactions between b. pertussis and 28 other bacterial species were studied by various quantitative immunoelectrophoretic methods. a sonicated b. pertussis antigen preparation and a corresponding pooled rabbit antiserum were used as reference system. two of the b. pertussis antigens were cross-reactive with antigens from 17, respectively 19, other bacterial species mainly gram-negative species. as judged by absorption of antibodies, the degree of cross-reactivity of these b. pertussis antig ... | 1976 | 187010 |
| role of dna repair in natural resistance of rat ascites hepatomas to nitrogen mustard. | mechanism of natural resistance of rat ascites hepatomas to nitrogen mustard was examined with tumors, especially ah-13 and ah-44, sensitive and resistant to the chemical, respectively. there was found little difference in the uptake and binding of the chemical among the two lines. in contrast, dna repair activity of the resistant line was higher than that of the sensitive line. this result suggested that natural resistance of ah-44 to the drug may be, at least partially, due to its higher activ ... | 1977 | 193752 |
| [acute diarrhea in childhood--new aspects]. | | 1977 | 202052 |
| neuromuscular transmission in arterioles of guinea-pig submucosa. | 1. intracellular recordings were made from arterioles lying in the submucosa of guinea-pig small intestine. 2. low frequency perivascular nerve stimulation evoked subthreshold excitatory junction potentials which facilitated. 3. higher frequency stimulation caused summation of excitatory junction potentials and the initiation of muscle action potentials. 4. arteriolar constriction was only observed following the initiation of a muscle action potential. | 1977 | 202698 |
| mammary tumors, hepatocellular carcinomas, and pancreatic islet changes in c3h-avy mice. | studies of tumor incidence and assorted lesions found in 187 c3h-avy mice throughout their natural life-spans revealed the following: hepatocellular carcinomas occurred in 54.3% of males, mammary carcinomas in 95% of females, pancreatic islet cell adenomas in 9.4% of males and in no females, and pancreatic islet cell hyperplasia in 41% of males and 23% of fefemales. islet cell hyperplasia and adenomas appeared to consist predominantly of alpha and delta cells. multiple tumors, or hyperplasia, or ... | 1978 | 203711 |
| intestinal calcium binding protein levels in the one month old turkey poult. | intestinal calcium binding protein cabp) was studied in one month old poults which had been fed a vitamin d3-deficient diet unsupplemented or with 399, 600, 900 or 1200 i.u. vitamin d3 added per kg. diet. cabp levels increased with vitamin d3 supplementation up to 600 i.u. but showed no further increase with higher dietary vitamin d3. poults supplemented with 600 to 1200 i.u. vitamin d3/kg. diet had an average of 1.03 mg. cabp/g. of duodenal mucosa cells collected. the calcium binding activity o ... | 1977 | 203920 |
| [diaphyseal bone tuberculosis]. | the authors report a case of diaphyseal bony tuberculosis, in which the diagnosis was not made for a long time as bony tuberculosis is considered exceptional and owing to the presence of a staphylococcus which oriented the diagnosis towards osteomyelitis. bony tuberculosis certainly affects more easily immigrant patients, in whom one may see multifocal forms, but also affects those who, by their environment, might imagine they are protected, and it is not exceptional to see unifocal forms. | 1977 | 204049 |
| polysomes and polysomal mrnas in sv40-infected cv-1 cells. in vivo observations. | sv40-specific polysomes are relatively larger than host polysomes. both 16 s and 19 s virus-specific late mrnas are found associated with these polysomes and they are present in a ratio of approximately 2 : 1. the rates at which these virus-specific mrnas are translated are the same, so that the relative amounts of virus-specific polypeptides synthesized in the virus-infected cells is determined by the relative amounts of 16 s and 19 s mrnas coding for them. | 1978 | 208616 |
| [human science. behavioral science. 4. social awareness and human behavior--a theme in sociological behavior theory concerning the patient]. | | 1977 | 242705 |
| a possible folding pathway of bovine pancreatic rnase. | a theoretical pathway for the folding of rnase into its native conformation is derived from the contact map computed from crystallographic coordinates. the pathway is based on the hypothesis of tanaka and scheraga, according to which localized conformations stabilized by short- and medium-range interactions form before those conformational features that are stabilized primarily by long-range interactions. the pathway deduced from the contact map agrees with experimental information on intermedia ... | 1979 | 293701 |
| biochemical and topographical studies on escherichia coli cell surface. iv. giant spheroplast formation from a filamentous cell. | long, nonseptate filamentous cells consisting of 5 to 40 single-cell unit lengths were formed from escherichia coli surface mutant ont-3 by treatment with a sublethal concentration of sodium dodecyl sylfate. as distinct from several other elongated cells (e.g., thymine-starved filaments), it was found here that stable giant spheroplasts, 5 to 10 micrometers in diameter, were produced by the action of lysozyme in the presence of bovine serum albumin via the gradual fusion of distinct spheroplasti ... | 1979 | 374397 |
| some effects of uncouplers and inhibitors on growth and electron transport in rumen bacteria. | uncouplers and inhibitors of electron transport affected growth and electron transport of rumen bacteria in various ways. selenomonas ruminantium was not affected by inhibitor and uncoupler concentrations which affected growth and electron transport of bacteroides ruminicola, b. succinogenes, and butyrivibrio fibrisolvens. inhibitors, when active, led to accumulation of reduced electron carriers before the site of action, but differences were found among organisms in the site of action of these ... | 1979 | 457609 |
| synthesis of alpha-ketoglutarate by reductive carboxylation of succinate in veillonella, selenomonas, and bacteriodes species. | evidence for reductive carboxylation of succinate to synthesize alpha-ketoglutarate was sought in anaerobic heterotrophs from the rumen and from other anaerobic habitats. cultures were grown in media containing unlabeled energy substrates plus [14c]succinate, and synthesis of cellular glutamate with a much higher specific activity than that of cellular asparate was taken as evidence for alpha-ketoglutarate synthase activity. our results indicate alpha-ketoglutarate synthase functions in selenomo ... | 1979 | 533772 |
| transformation of mercuric chloride and methylmercury by the rumen microflora. | the microflora in strained rumen fluid did not methylate or volatilize 203hg2+ at detectable rates. however, there was an exponential decay in the concentration of added ch3hg+, which was attributed to demethylation. the major product of demethylation was metallic mercury (hg0), and it was released as a volatile product from the reaction mixture. demethylation occurred under both anaerobic and aerobic conditions. the rate of demethylation was proportional to the concentration of added ch3hg+-hg ... | 1979 | 539820 |
| alkaline phosphatase activity of rumen bacteria. | of the 54 strains of rumen bacteria examined for alkaline phosphatase (apase) production, 9 of 33 gram-negative strains and none of 21 gram-positive strains produced the enzyme. the apase of the cells of the three strains of bacteroides ruminicola that produced significant amounts of the enzyme was located in the periplasmic area of the cell envelope, whereas the enzyme was located in the strains of selenomonas ruminantium and succinivibrio dextrinosolvens was associated with the outer membrane. ... | 1977 | 563216 |
| effects of heavy metals and other trace elements on the fermentative activity of the rumen microflora and growth of functionally important rumen bacteria. | the inhibitory effects of high concentrations of essential and non-essential trace elements were tested on the rumen microflora using the rate of fermentation in vitro as the assay. the elements (and the concentration causing 50% inhibition) in decreasing order of toxicity were hg2+ (20 microgram/ml), cu2+ (21 microgram/ml), cr6+ (70 microgram/ml), se4+ (73 microgram/ml), ni2+ (160 microgram/ml), cd2+ (175 microgram/ml), as3+ (304 microgram/ml) and as5+ (1610 microgram/ml). the elements tested t ... | 1978 | 565671 |
| production of branched-chain volatile fatty acids by certain anaerobic bacteria. | net production of isobutyric acid, isovaleric acid, and 2-methylbutyric acid by cultures of bacteroides ruminicola and megasphaera elsdenii on media that contained trypticase or casein hydrolysate continued (up to 5 days) after growth had ceased. only trace quantities of these acids were produced in a medium that contained a mixture of amino acids that did not include the branched-chain amino acids. m. elsdenii produced increased quantities of the branched-chain fatty acids in a medium that cont ... | 1978 | 566082 |
| lactic acid-utilizing bacteria in ruminal fluid of a steer adapted from hay feeding to a high-grain ration. | a mature, rumen-cannulated steer fed coastal bermuda grass hay for 8 weeks was adapted to a high-grain ration by stepwise increases in grain over a period of 4 weeks. the grain rations had concentrate-to-roughage ratios of 40:60, 70:30, and 85:15 and were fed for 10, 7, and 11 days, respectively. numerical estimates of lactate-utilizing bacteria in ruminal fluid of the steer the last 3 days each ration was fed were made by colony counts. lactate-utilizing bacteria were identified as megasphaera ... | 1976 | 1275348 |
| dap-decarboxylase activity and lysine production by rumen bacteria. | the last step of pathway of lysine biosynthesis by rumen bacteria was tested. the first measurements of dap-decarboxylase activity and of lysine production by megasphera elsdenii, selenomonas ruminantium, clostridium spp., butyrivibrio fibrisolvens and bacteroides succinogenes as well as the first attempts to increase the lysine production by ruminal streptococci by mutation are described. the highest values were measured in selenomonas ruminantium (dap-decarboxylase activity = 146 micrograms da ... | 1992 | 1295484 |
| complete nucleotide sequence of a selenomonas ruminantium plasmid and definition of a region necessary for its replication in escherichia coli. | a plasmid from selenomonas ruminantium subspecies lactilytica has been subcloned in escherichia coli k-12 and completely sequenced. three open reading frames (orfs) of 909, 801, and 549 bp were identified and the complete sequence was analyzed by comparison with dna and protein databases. no significant deoxynucleotide or amino acid sequence homology with other published genes or proteins was detected. the plasmid was shown to replicate independently in e. coli k-12 by a dna polymerase i-depende ... | 1992 | 1409969 |
| interaction of ruminal bacteria in the production and utilization of maltooligosaccharides from starch. | the degradation and utilization of starch by three amylolytic and one nonamylolytic species of ruminal bacteria were studied. pure cultures of streptococcus bovis jb1, butyrivibrio fibrisolvens 49, and bacteroides ruminicola d31d rapidly hydrolyzed starch and maltooligosaccharides accumulated. the major starch hydrolytic products detected in s. bovis cultures were glucose, maltose, maltotriose, and maltotetraose. in addition to these oligosaccharides, b. fibrisolvens cultures produced maltopenta ... | 1992 | 1539992 |
| enzymes associated with metabolism of xylose and other pentoses by prevotella (bacteroides) ruminicola strains, selenomonas ruminantium d, and fibrobacter succinogenes s85. | prevotella (bacteroides) ruminicola strains b(1)4 and s23 and selenomonas ruminantium strain d used xylose as the sole source of carbohydrate for growth, whereas fibrobacter succinogenes was unable to metabolize xylose. prevotella ruminicola strain b(1)4 exhibited transport activity for xylose. in contrast, f. succinogenes lacked typical xylose uptake activity but did exhibit low binding potential for the sugar. prevotella ruminicola strains b(1)4 and s23 as well as s. ruminantium d showed low x ... | 1992 | 1643581 |
| utilization of nucleic acids by selenomonas ruminantium and other ruminal bacteria. | species of ruminal bacteria were screened for the ability to grow in media containing rna or dna as the energy source. bacteroides ruminicola d31d and selenomonas ruminantium hd4, ga192, and d effectively used rna for growth, but not dna. b. ruminicola d31d was able grow on nucleosides but not on bases or ribose. the s. ruminantium strains were able to grow when provided with either nucleosides or ribose but not bases. strains of s. ruminantium, but not b. ruminicola d31d, were also able to use ... | 1990 | 1707252 |
| effect of a saccharomyces cerevisiae culture on lactate utilization by the ruminal bacterium selenomonas ruminantium. | the objective of this study was to examine the effects of a saccharomyces cerevisiae culture (yea-sacc) on lactate utilization by the predominant ruminal bacterium selenomonas ruminantium. lactate uptake was stimulated by yea-sacc concentrations between 2.5 and 10 g/liter, and the 5-g/liter level increased uptake 3.8-fold. when yea-sacc concentrations were increased above the 5-g/liter level lactate uptake was decreased, but 10 g/liter still stimulated uptake more than threefold. a filter-steril ... | 1991 | 1752834 |
| intracellular ph of acid-tolerant ruminal bacteria. | acid-tolerant ruminal bacteria (bacteroides ruminicola b1(4), selenomonas ruminantium hd4, streptococcus bovis jb1, megasphaera elsdenii b159, and strain f) allowed their intracellular ph to decline as a function of extracellular ph and did not generate a large ph gradient across the cell membrane until the extracellular ph was low (less than 5.2). this decline in intracellular ph prevented an accumulation of volatile fatty acid anions inside the cells. | 1991 | 1781695 |
| localization of bacterial antigens in calves inoculated orally with ruminal bacteroides succinogenes and selenomonas ruminantium. | localization of bacteria and bacterial materials was investigated in calves inoculated orally with live organisms of both bacteroides succinogenes and selenomonas ruminantium by a immunohistological method using rabbit antiserum against the outer membrane of those organisms and by a scanning electron microscope. the intact organisms of both inoculated bacterial species were detected on the rumen wall and in the lamina propria of the forestomach, and s. ruminantium also in the lymph nodes associa ... | 1990 | 2202844 |
| potential anti-hiv active pyranoid analogs of azt. | | 1990 | 2206706 |
| cytotoxic activity against hiv-infected monocytes by recombinant interleukin 2-activated natural killer cells. | natural killer (nk) cells have long been known to aid in the control of viral infections by killing virus-infected cells, including those infected with human immunodeficiency virus (hiv). among the possible nk-susceptible target cells in an infected individual, the monocyte/macrophages are of special significance since they may serve as both a reservoir of hiv and aid in dissemination of the virus throughout the body. a new technique for the enrichment and cultivation of large numbers of recombi ... | 1990 | 2223237 |
| cytotoxic activity against hiv-infected monocytes by recombinant interleukin 2-activated natural killer cells. | natural killer (nk) cells have long been known to aid in the control of viral infections by killing virus-infected cells, including those infected with human immunodeficiency virus (hiv). among the possible nk-susceptible target cells in an infected individual, the monocyte/macrophages are of special significance since they may serve as both a reservoir of hiv and aid in dissemination of the virus throughout the body. a new technique for the enrichment and cultivation of large numbers of recombi ... | 1990 | 2223237 |
| folding and stability of trp aporepressor from escherichia coli. | equilibrium and kinetic studies of the urea-induced unfolding of trp aporepressor from escherichia coli were performed to probe the folding mechanism of this intertwined, dimeric protein. the equilibrium unfolding transitions at ph 7.6 and 25 degrees c monitored by difference absorbance, fluorescence, and circular dichroism spectroscopy are coincident within experimental error. all three transitions are well described by a two-state model involving the native dimer and the unfolded monomer; the ... | 1990 | 2223756 |
| folding and stability of trp aporepressor from escherichia coli. | equilibrium and kinetic studies of the urea-induced unfolding of trp aporepressor from escherichia coli were performed to probe the folding mechanism of this intertwined, dimeric protein. the equilibrium unfolding transitions at ph 7.6 and 25 degrees c monitored by difference absorbance, fluorescence, and circular dichroism spectroscopy are coincident within experimental error. all three transitions are well described by a two-state model involving the native dimer and the unfolded monomer; the ... | 1990 | 2223756 |
| detection of bovine antibodies to the outer membrane of ruminal bacteroides succinogenes by enzyme-linked immunosorbent assay (elisa). | the enzyme-linked immunosorbent assay (elisa) was used to detect bovine serum antibodies directed to the outer membrane antigen of a ruminal bacteria, bacteroides succinogenes. the outer membrane antigen of b. succinogenes was highly reactive against homologous antiserum, compared with rabbit sera raised against b. ruminicola subsp. ruminicola, b. ruminicola subsp. brevis and selenomonas ruminantium. the titers of sera from colostrum-deprived calves were negligible level, while those of sera fro ... | 1990 | 2313950 |
| monoclonal antibodies against the ruminal bacterium selenomonas ruminantium. | monoclonal antibodies were raised against whole cells of two different strains of selenomonas ruminantium and tested for specificity and sensitivity in immunofluorescence and enzyme-linked immunosorbent assay procedures. species-specific and strain-specific antibodies were identified, and reactive antigens were demonstrated in solubilized cell wall extracts of s. ruminantium. a monoclonal antibody-based solid-phase immunoassay was established to quantify s. ruminantium in cultures or samples fro ... | 1990 | 2389936 |
| white noise analysis of temporal properties in simple receptive fields of cat cortex. | we studied the linear and nonlinear temporal response properties of simple cells in cat visual cortex by presenting at single positions in the receptive field an optimally oriented bar stimulus whose luminance was modulated in a random, binary fashion. by crosscorrelating a cell's response with the input it was possible to obtain the zeroth-, first-, and second-order wiener kernels at each rf location. simple cells showed pronounced nonlinear temporal properties as revealed by the presence of pr ... | 1990 | 2390534 |
| effect of direct-fed microbials on rumen microbial fermentation. | nonbacterial, direct-fed microbials added to ruminant diets generally consist of aspergillus oryzae fermentation extract, or saccharomyces cerevisiae cultures, or both. results from in vivo research have been variable regarding effects of direct-fed microbials on ruminant feedstuff utilization and performance. some research has shown increased weight gains, milk production, and total tract digestibility of feed components, but others have shown little influence of direct-fed microbials on these ... | 1992 | 1500571 |
| mechanism of action of d-galacturonan digalacturonohydrolase of selenomonas ruminantium on oligogalactosiduronic acids. | the mechanism of action of the specific d-galacturonan digalacturonohydrolase [poly-(1----4)-alpha-d-galactosiduronate digalacturonohydrolase, ec 3.2.1.82] of selenomonas ruminantium was investigated by using reducing-end [1-3h]-labeled oligogalactosiduronates having degree of polymerization 3-5 as the substrates. the reaction products, incorporation and distribution of radioactivity in products, and the frequency of oligogalactosiduronate bond-cleavage were quantitatively estimated as functions ... | 1992 | 1473108 |
| characterization of a plasmid from the ruminal bacterium selenomonas ruminantium. | a 4.8-kilobase-pair plasmid was isolated from the ruminal bacterium selenomonas ruminantium hd4 by using a sodium carbonate-edta washing buffer to improve cell lysis (r.g. dean, s.a. martin, and c. carver, lett. appl. microbiol. 8:45-48, 1989). this plasmid, designated psr1, appears to be quite stable. no evidence of plasmid dna was detected in s. ruminantium d or ga192. all three strains were tested for antibiotic resistance, and all were kanamycin resistant (mic, 25 to 50 micrograms/ml). only ... | 1989 | 2619301 |
| the pectinolytic enzyme of selenomonas ruminantium. | a cell-bound pectinolytic enzyme was isolated from cells of selenomonas ruminantium and purified about 360-fold. the optimum ph and temperature for enzyme activity was 7.0 and 40 degrees c. the enzyme degraded polymeric substrates by hydrolysis of digalacturonic acid units from the non-reducing end; the best substrate was nonagalacturonic acid. unsaturated trigalacturonate was also degraded, but 30% slower than the saturated analogue. the enzyme was classified as a poly (1,4-alpha-d-galactosidur ... | 1989 | 2708171 |
| the characterization and ultrastructure of two new strains of butyrivibrio. | strains b-385-1 and 2-33 are numerically important components rumen bacterial populations , but they have remained (taxonomically) undefined. in spite of some resemblance to selenomonas ruminantium in their cell size and in their formation of tufts of flagella, they more closely resemble butyrivibrio fibrisolvens in the subpolar location of their flagella, in their guanine + cytosine content, and in most biochemical characteristics, including butyrate formation. cells of these strains stain gram ... | 1989 | 2743214 |
| effects of methanol on the growth of gastrointestinal anaerobes. | the effects of methanol on the growth of representative, predominant, anaerobic gut bacteria were studied. growth yields and rates were determined in a base medium to which methanol was added to produce media with methanol concentrations varying, in twofold steps, over a concentration range of 0.01 to 25%, by volume. the growth of many of the organisms was completely inhibited by a methanol concentration equal to, or less than, 6.2%. isolates representing cellulolytic species were completely inh ... | 1989 | 2743215 |
| the dilation of arterioles in frog submaxillaris muscle caused by visible light. | transillumination of submaxillaris muscle with incandenscent lamp led to arteriolar dilation if the illuminance exceeded 1-2 x 10(3) lux or reached the time-mean value of 3 x 10(4) lux when a flash lamp was used (pulse duration 10 microseconds, repetition rate 50 s-1). the temperature increase within a light spot on the muscle did not exceed 0.1 degrees c, visible light being the only reason of the dilation of arterioles. at 6.3 x 10(4) lux (incandescent lamp, green filter) the latency of dilati ... | 1989 | 2786511 |
| use of antibiotic resistance mutations to track strains of obligately anaerobic bacteria introduced into the rumen of sheep. | selective plating procedures were used to follow the fate of rifampicin-resistant mutant strains of the obligately anaerobic species bacteroides multiacidus and selenomonas ruminantium after their introduction at numbers around 10(7)/ml into the rumen of sheep. bacteroides multiacidus strain f100 showed an initially rapid rate of loss (49%/h) but subsequently numbers declined more gradually approaching the limits of detection (less than 10(3)/ml) after 100 h. viable cell numbers also decreased i ... | 1989 | 2808185 |
| effect of dietary copper sulfate, aureo sp250, or clinoptilolite on ureolytic bacteria found in the pig large intestine. | the predominant ureolytic bacteria in the pig large intestine were determined while growing pigs were fed a basal diet or basal diet plus copper sulfate, aureo sp250, or clinoptilolite. fecal samples were collected from four pigs fed each diet at 3, 9, and 14 weeks and analyzed for total colony counts and percent ureolytic bacteria. fecal urease activity, ammonia nitrogen, and identity of the ureolytic bacteria were determined at 14 weeks. copper sulfate and aureo sp250 reduced the number of ure ... | 1987 | 2823707 |
| isolation and characterization of a temperate bacteriophage from the ruminal anaerobe selenomonas ruminantium. | a temperate bacteriophage was obtained from an isolate of the ruminal anaerobe selenomonas ruminantium. clear plaques that became turbid on further incubation occurred on a lawn of host bacteria. cells picked from a turbid plaque produced healthy liquid cultures, but these often lysed on storage. mid-log-phase liquid cultures incubated with the bacteriophage lysed and released infectious particles with a titer of up to 3 x 10(7) pfu/ml. a laboratory strain of s. ruminantium, hd-4, was also sensi ... | 1988 | 2843096 |
| h2 production by selenomonas ruminantium in the absence and presence of methanogenic bacteria. | selenomonas ruminantium is a nonsporeforming anaerobe that ferments carbohydrates primarily to lactate, propionate, acetate and co2. h2 production by this species has not been previously reported. we found, however, that some strains produce trace amounts of h2 which can be detected by sensitive gas chromatographic procedures. h2 production is increased markedly, in some cases almost 100-fold, when the selenomonads are co-cultured with methane-producing bacteria. growth of the methane-producing ... | 1975 | 804850 |
| effect of phenobarbital on the gamma-glutamyltranspeptidase activity and the remodeling of nodules induced by the initiation-selection model. | the effect of subsequent administration of phenobarbital on the gamma-glutamyltranspeptidase (ggt) activity and on the remodeling of nodules induced by the solt-farber procedure was examined in rats. ggt-nodules were initiated by diethylnitrosamine (dena) followed by selection with 2-acetylaminofluorene (2aaf) in the diet and a partial hepatectomy (ph). phenobarbital (500 ppm in the drinking water), administered to rats that were previously treated according to the solt-farber procedure, (1) inc ... | 1985 | 2860967 |
| cadaverine covalently linked to a peptidoglycan is an essential constituent of the peptidoglycan necessary for the normal growth in selenomonas ruminantium. | cadaverine links covalently to the d-glutamic acid residue of the peptidoglycan in selenomonas ruminantium, a strictly anaerobic gram-negative bacterium (kamio, y., itoh, y., and terawaki, y. (1981) j. bacteriol. 146, 49-53). this report clarifies a physiological function of cadaverine in this organism by using dl-alpha-difluoromethyllysine, which had previously been shown to be a selective irreversible inhibitor of lysine decarboxylase of mycoplasma dispar (pösö, h., macann, p.p., tanskanen, r. ... | 1986 | 3084485 |
| susceptibility and resistance of ruminal bacteria to antimicrobial feed additives. | susceptibility and resistance of ruminal bacterial species to avoparcin, narasin, salinomycin, thiopeptin, tylosin, virginiamycin, and two new ionophore antibiotics, ro22-6924/004 and ro21-6447/009, were determined. generally, antimicrobial compounds were inhibitory to gram-positive bacteria and those bacteria that have gram-positive-like cell wall structure. mics ranged from 0.09 to 24.0 micrograms/ml. gram-negative bacteria were resistant at the highest concentration tested (48.0 micrograms/ml ... | 1987 | 3116929 |
| influence of ch4 production by methanobacterium ruminantium on the fermentation of glucose and lactate by selenomonas ruminantium. | a method is described for increasing the production of h2 from glucose or lactate by selenomonas ruminantium by sequential transfers in media containing pregrown methanobacterium ruminantium. the methanogen uses the h2 formed by the selenomonad to reduce co2 to ch4. analysis of fermentation products from glucose showed that lactate was the major product formed from glucose by s. ruminantium alone. several sequential transfers in the presence of the methanogen caused a marked decrease in lactate ... | 1977 | 596874 |
| demonstration of 5'-methylthioadenosine phosphorylase activity in various rat tissues. some properties of the enzyme from rat lung. | an enzyme (5'-methylthioadenosine phosphorylase) that catalyzes the phosphorolytic cleavage of 5'-methylthioadenosine to 5-methylthioadenosine to 5-methylthioribose-1-phosphate and adenine was found in various rat tissues. liver and lung had the highest enzyme activities and heart the lowest, most of the activity (greater than 90%) was recovered in soluble tissue fractions. the enzyme from rat lung was purified about 30-fold by ph treatment (nh4)2so4 fractionation, and gel filtration. the enzyme ... | 1978 | 415762 |
| [the occurrence and properties of selenomonas ruminantium bacteria in calves during the time of milk feedings]. | the occurrence, morphological properties, urease and alpha-amylase activities of selenomonas ruminantium were investigated in calves in the period of milk nutrition. the average number of the organisms was found to range between 10(7) and 10(8) per 1 millilitre of rumen contents. the alpha-amylase activity of the selenomonas strains ranged from 0.1 to 8.8 ncat.ml-1 whereas their urease activity ranged from 6.3 to 261 ncat.ml-1 of nutrient medium. the presence of morphologically typical selenomon ... | 1988 | 3129854 |
| intravenous immune globulin in symptomatic paediatric human immunodeficiency virus infection. | seven paediatric patients with symptomatic human immunodeficiency virus (hiv) infection were prospectively studied for 6-24 months after the start of intravenous immune globulin (ivig) therapy. there was substantial clinical benefit during ivig treatment with marked reduction of febrile and infectious episodes, normalization of physical and psychomotor development, and absence of mortality. the immunologic monitoring revealed some discrete objective improvements. the results of this study favour ... | 1988 | 3134244 |
| thyrotrophin releasing hormone-induced growth hormone and prolactin release: physiological studies in intact rats and in hypophysectomized rats bearing an ectopic pituitary gland. | to determine how the sensitivity of the ectopic anterior pituitary gland to the gh-releasing effect of thyrotrophin releasing hormone (trh) might be affected by the time lapse from transplantation, trh (0-15 and 0-6 microng) was injected i.v. into hypophysectomized (hypox)-transplanted rats under urethane anaesthesia 1,3,8,15,30 and 60 days after transplantation, and plasma samples were taken 5 and 10 min later. baseline gh values gradually decreased with time from about 16-0 ng/ml (1 day) to ab ... | 1977 | 404376 |
| inactive mrna-protein complexes from mouse sarcoma-180 ascites cells. | mouse sarcoma ascites cells do not utilize fully their capacity for protein synthesis. a considerable portion of their ribosomes occur as inactive monomers. also, a substantial amount of the cellular mrna is in the form of ribonucleoprotein particles that sediment in the 20-70s range. this is indicated both by measurements of poly(a) content and by translation of the rna in cell-free systems. the population of polypeptides synthesized under the direction of the rna from these particles is less h ... | 1979 | 293664 |
| esterase activities in butyrivibrio fibrisolvens strains. | thirty strains of butyrivibrio fibrisolvens isolated in diverse geographical locations were examined for esterase activity by using naphthyl esters of acetate, butyrate, caprylate, laurate, and palmitate. all strains possessed some esterase activity, and high levels of activity were observed with strains 49, h17c, s2, actf2, and lm8/1b. esterase activity also was detected in other ruminal bacteria (bacteroides ruminicola, selenomonas ruminantium, ruminobacter amylophilus, and streptococcus bovis ... | 1988 | 3178205 |
| regulation of carbon flow in selenomonas ruminantium grown in glucose-limited continuous culture. | we have applied a model that permits the estimation of the sensitivity of flux through branch point enzymes (d. c. laporte, k. walsh, and d. e. koshland, j. biol. chem. 259:14068-14075, 1984) in order to analyze the control of flux through the lactate-acetate branch point of selenomonas ruminantium grown in glucose-limited continuous culture. at this branch point, pyruvate is the substrate of both the nad-dependent l-(+)-lactate dehydrogenase (ldh) and the pyruvate:ferredoxin oxidoreductase (pfo ... | 1988 | 3182729 |
| effects of the ionophores monensin and tetronasin on simulated development of ruminal lactic acidosis in vitro. | a continuous coculture of four ruminal bacteria, megasphaera elsdenii, selenomonas ruminantium, streptococcus bovis, and lactobacillus sp. strain lb17, was used to study the effects of the ionophores monensin and tetronasin on the changes in ruminal microbial ecology that occur during the onset of lactic acidosis. in control incubations, the system simulated the development of lactic acidosis in vivo, with an initial overgrowth of s. bovis when an excess of glucose was added to the fermentor. la ... | 1988 | 3223764 |
| functionally polarized layers formed by epidermal cells on a permeable transparent collagen film. | rat epidermal cells were cultured on a transparent collagen film, which was permeable to low mr substances. then the cells were bathed in both media (apical and basal), which were separated by the collagen membrane. the cells formed a multi-layered epidermal sheet with well-developed structures of desmosomes. this sheet on a permeable support was found to be an effective permeability barrier for glucose and amino acids. the epidermal layer showed functional polarity for the uptake and excretion ... | 1988 | 3255753 |
| suppression of phytohemagglutinin response by fungi from a "leukemia" house. | fungal isolates from the house of a husband and wife who both developed acute myelomonocytic leukemia were assayed for effects on the in vivo response to phytohemagglutinin in guinea pigs. skin responses to intradermal phytohemagglutinin were measured following injections of sterile fungal extracts. isolates of penicillium canescens, curvularia, fusarium sambucinum, fusarium equiseti and trichoderma koningii from the leukemia-associated house depressed the responses to phytohemagglutinin, but no ... | 1979 | 291385 |
| on the significance of the increase in homovanillic acid (hva) caused by antipsychotic drugs in corpus striatum and limbic forebrain. | the effect of various antipsychotic drugs on the blockade of dopaminergic receptors in striatum and limbic forebrain was examined by establishing dose-response curves for the increase in hva and for the antagonism of d-amphetamine-induced rotation in rats with unilateral lesions of the substantia nigra. a good quantitative correlation was found between dopaminergic blockade in the striatum as reflected by the ed100 for striatal hva increase and the ed50 for rotational antagonism and the occurren ... | 1975 | 242028 |
| [nosocomial infections of the urinary tract in urology patients]. | researches effected in different nations have revealed that 5-12% of all hospitalized patients was affected with a nosocomial infection and that almost 1/3 of these were urinary tract infections. the hospitalized urological patients have a considerable proneness to contract a nosocomial urinary tract infection, because they are subjected to transurethral or suprapubic bladder catheterisms, to transurethral surgery and to the endoscopies, that are promoting maneuvers the urinary tract infections. ... | 1988 | 3340313 |
| differentiation of ruminal bacterial species by enzyme-linked immunosorbent assay using egg yolk antibodies from immunized chicken hens. | cross-reactivity among four species of ruminal bacteria was examined by using egg yolk antibodies from immunized leghorn laying hens and an enzyme-linked-immunosorbent assay. the effects of the four species on the hens were compared on various days postimmunization. hens injected with the same bacterial species had similar apparent antibody levels over the entire postimmunization period, but only bacteroides ruminicola b1(4) and selenomonas ruminantium d antigens elicited early increases in appa ... | 1988 | 3355145 |
| the potential histogenic relationship of the peripheral nerve to synovioma. | the belief that synoviomas differentiate from the synovial membrane has been widely accepted. absolute proof is lacking, mainly because precancerous synovial atypia has not been documented in human tissue. during the last 50 years, pain has emerged as one of the significant features of synovioma, occurring very early in the course of the disease in some patients. gross relationship to nerve, cumulative histological data, and theoretical relationships with three other cancers of neural origin sug ... | 1979 | 225019 |
| pathobiology of cortical neurons in metabolic and unclassified amentias. | visualization of the neuron in its entirety through the use of the rapid golgi method has permitted detection of several pathobiological features of neurons that are intimately associated with profound mental retardation in infants and children. in cases of unclassified mental retardation, dendrites and particularly dendritic spines exhibit severe developmental abnormalities. dendritic spines, the postsynaptic components of axospinodendritic synapses, may be absent or abnormally long and thin in ... | 1979 | 217050 |
| t-2 toxin metabolism by ruminal bacteria and its effect on their growth. | the effect of t-2 toxin on the growth rates of different bacteria was used as a measure of its toxicity. toxin levels of 10 micrograms/ml did not decrease the growth rate of selenomonas ruminantium and anaerovibrio lipolytica, whereas the growth rate of butyrivibrio fibrisolvens was uninhibited at toxin levels as high as 1 mg/ml. there was, however, a noticeable increase in the growth rate of b. fibrisolvens ce46 and ce51 and s. ruminantium in the presence of low concentrations (10 micrograms/ml ... | 1987 | 3579272 |
| [mononucleosis syndrome and thrombocytopenic purpura in q fever (rickettsia burneti)]. | case of a patient with a mononucleosis syndrome and thrombocytopenic purpura during q fever. this etiology could lead to a systematic search for a rickettsial cause for such a symptomatology with a negative paul-bunnell-davidson reaction. | 1978 | 211604 |
| fibrous histiocytoma of the tarsus. | a 21-year-old white man had an asymptomatic eyelid mass that had slowly enlarged for one year. on clinical examination the lesion was well circumscribed; it appeared subconjunctivally but provoked no conjunctival inflammation, and had a consistency suggestive of cartilage. microscopic examination showed it was matted and composed of elongated fibro-histiocytes and an evenly distributed population of xanthoma cells and touton giant cells. the lesion was easily removed by local excision because of ... | 1977 | 202162 |
| purification of a nickel-containing urease from the rumen anaerobe selenomonas ruminantium. | urease was purified 592-fold to homogeneity from the anaerobic rumen bacterium selenomonas ruminantium. the urease isolation procedure included a heat step and ion-exchange, hydrophobic, gel filtration, and fast protein liquid chromatography. the purified enzyme exhibited a km for urea of 2.2 +/- 0.5 mm and a vmax of 1100 mumol of urea min-1 mg-1. the molecular mass estimated for the native enzyme was 360,000 +/- 50,000 daltons, whereas a subunit value of 70,000 +/- 2,000 daltons was determined. ... | 1986 | 3711113 |
| stoichiometry of glucose and starch splitting by strains of amylolytic bacteria from the rumen and anaerobic digester. | the stoichiometry of glucose and starch splitting by the amylolytic bacteria streptococcus bovis, selenomonas ruminantium, butyrivibrio fibrisolvens, eubacterium ruminantium and clostridium sp. was followed. there were many differences in the ratios of metabolites and in growth yields, as well as in the cell composition, between the growth on glucose and starch. the bacteria employ different nutritional strategies with respect to both energy sources. | 1986 | 3759723 |
| particulate carbonic anhydrase in homogenates of human kidney. | about 2% of human kidney carbonic anhydrase (carbonate hydro-lyase, ec 4.2.1.1) has been found in particulate fractions. its distribution in the particulate fractions obtained by differential centrifugation suggests that it may be concentrated in the brush border. the particulate enzyme is like red cell carbonic anhydrace c in its susceptibility to inhibition by anions. particulate carbonic anhydrase is firmly bound to the membrane and is not released by incubation at ph 10.6 and 37 degrees c or ... | 1976 | 184830 |
| phosphoenolpyruvate-dependent phosphorylation of hexoses by ruminal bacteria: evidence for the phosphotransferase transport system. | six species of ruminal bacteria were surveyed for the phosphoenolpyruvate (pep)-dependent phosphorylation of glucose. selenomonas ruminantium hd4, streptococcus bovis jb1, and megasphaera elsdenii b159 all showed significant activity, but butyrivibrio fibrisolvens 49, bacteroides succinogenes s85, and bacteroides ruminicola b1(4) showed low rates of pep-dependent phosphorylation and much higher rates in the presence of atp. s. ruminantium hd4, s. bovis jb1, and m. elsdenii b159 also used pep to ... | 1986 | 3789722 |
| the fine structure of a tri-lamellar body in various species of anabaena. | | 1976 | 184493 |
| theoretical mechanisms for synthesis of carcinogen-induced embryonic proteins: xiv. mutational and non-mutational mechanisms as subsets of a more general mechanism. part c. a defined cancer mutation. | the results of applying a mechanism of ethionine-induced embryonic gene derepressions to explain similar features found in hereditary tyrosinemia have been extended to a well defined cancer mutation. in all three cases, the described mechanism is compatible with the explanation for the etiology of embryonic like phenotypic expressions in cells and potentially for the carcinogenic process. the essence of the formulated process for a human bladder carcinoma mutation in the ras gene for a protein p ... | 1985 | 3853079 |
| human plasma lipoproteins as accelerators of prothrombin activation. | the activation rate of bovine prothrombin by factor xa and ca2+ has long been known to be greatly enhanced by addition of phospholipid. upon substitution of human plasma lipoproteins for phospholipid (cephalin) in this activation system, only very low density lipoprotein enhances prothrombin activation. low density lipoprotein and high density lipoprotein have no stimulatory effect on prothrombin activation. on the other hand, the sonicated lipid extracts from very low, low, and high density lip ... | 1976 | 182693 |
| control of lactate production by selenomonas ruminantium: homotropic activation of lactate dehydrogenase by pyruvate. | selenomonas ruminantium produced one mole of d(-)-lactate per mole of glucose used at all dilution rates in ammonia-limited continuous culture. in contrast, lactate production varied according to the dilution rate when glucose was the limiting nutrient. at dilution rates of less than 0.2 h-1, acetate and propionate were the main fermentation products and lactate production was low. at dilution rates above 0.2 h-1, the pattern changed to one of high lactate production similar to that under ammoni ... | 1978 | 103995 |
| the rumen microbiology of seaweed digestion in orkney sheep. | the microbial populations of the rumens of seaweed-fed and pasture-fed orkney sheep were examined. the populations in the pasture-fed sheep were similar to those of other domestic ruminants fed on land plants, but those of the seaweed-fed animals showed major differences in the dominant species. total ciliate populations were quantitatively similar, but in the seaweed-fed animals dasytricha ruminantium was one of the most dominant species. no phycomycete fungi or cellulolytic bacteria were found ... | 1985 | 4030526 |
| heterogeneity of alkaline phosphatase observed by high-performance liquid chromatography. | | 1985 | 4030971 |
| x-ray tv system for measuring microcirculation in small pulmonary vessels. | we developed a new system that consists of 1) a specially designed x-ray apparatus, 2) an x-ray-sensitive 1-in. vidicon camera, and 3) a digital image-processing device. the picture element is approximately 20 micron in size, and the time required for one frame is 1/30 s. using this system, we measured the internal diameter (id), the cross-sectional area, flow velocity, volume flow, and transit time of small pulmonary vessels of approximately 100-500 micron at control and with serotonin in anest ... | 1985 | 4055558 |
| ultrastructural observations on the sporogony of eimeria maxima. | | 1985 | 4093234 |
| analysis of bypass activation of c3 by endotoxic lps and loss of this potency. | | 1973 | 4122408 |
| isolation of ureolytic peptostreptococcus productus from feces using defined medium; failure of common urease tests. | colony counts of fecal samples from three persons, obtained by using a chemically defined anaerobic roll-tube medium (containing glucose, maltose, glycerol, minerals, hemin, b-vitamins, methionine, volatile fatty acids, sulfide, bicarbonate, agar, carbon dioxide (gas phase), and 1 mm nh(4) (+) as main nitrogen source), averaged 60% of the 8.8 x 10(10) bacteria per g obtained when 0.2% trypticase and 0.05% yeast extract were added to the otherwise identical medium. when 0.2% vitamin-free casitone ... | 1974 | 4213672 |
| the effect of acid mucopolysaccharides on the activation energy of collagen. | | 1967 | 4227781 |
| atpase activity and palmitate oxidation of hepatic mitochondria in energy production in endotoxemia. | | 1971 | 4256350 |