| characterization of 40,000- and 25,000-dalton intermediate precursors to rauscher murine leukemia virus gag gene products. | under steady-state labeling conditions, rauscher murine leukemia virus-infected nih swiss mouse cells contain at least three major polyproteins derived from the viral gag gene. they have molecular weights of 65,000, 40,000, and 25,000. they have been termed ppr65gag, pr40gag, and ppr25gag. ppr65gag has been shown by a number of laboratories to be composed of all four core proteins (p15, pp12, p30, and p10). in this paper, pr40gag was found to contain p30 and p10 antigenic determinants and peptid ... | 1979 | 94357 |
| a biochemical comparison of development of various facial bones in neonatal rats. | | 1979 | 94540 |
| fibrinogen turnover in pregnant rabbits during the first and last thirds of gestation. | although thrombin-mediated fibrinogen derivatives have been observed in normal pregnancy, an increased turnover of fibrinogen has been suspected but has not yet been demonstrated. fibrinogen turnover was studied in 14 pregnant and 10 nonpregnant female rabbits by use of purified rabbit 125i-labeled fibrinogen. in the last third of gestation the half life of fibrinogen was shortened by 45% and the fractional catabolic rate increased by 44% in comparison to the values for the first third of gestat ... | 1978 | 99036 |
| nature's infant nutrition. breast feeding: a means of imparting immunity? | | 1978 | 99622 |
| the measurement of haemagglutinin and matrix protein present on the surface of influenza virus infected p815 mastocytoma cells. | a thermodynamic approach has been used to measure the amount of haemagglutinin and matrix protein expressed at the surface of p815 cells infected for periods between 4.5 and 11 h with either wsn (h0n1) or jap (h2n2) strains of type a influenza virus. this involved measuring the interaction of different concentrations of labelled (fab)2 preparations of specific antibody with normal and infected cells. assuming that one molecule of (fab)2 bound to one molecule of antigen, values for the number of ... | 1979 | 107273 |
| pseudomonas orbital cellulitis. | a 3-month-old infant being treated for bronchitis developed a rapid onset but otherwise typical orbital cellulitis. because gram-negative infections and septicemia are common occurrences in the newborn nursery, this patient was given systemic gentamicin and ampicillin. sinus x-rays were not attempted. two days after treatment the eyelids were opened. a strikingly large corneal ulcer with perforated globe and endophthalmitis was found. pseudomonas aeroginosa was cultured from the blood, conjuncti ... | 1979 | 107805 |
| [period of sensitivity of the rabbit fetus to the embryopathic and lethal action of d-glucose in intraovular injection]. | we studied the effect of injection of d-glucose in rabbit foetus ovulary liquid at the 14th, 16th and 18th day of pregnancy. injection of 14th and 16th day determined a high percentage of foetal death and limb necrosis. on and after the 18th day of pregnancy, the foetus is insensible to the treatment. the 18th day is, in the rabbit, the period of a new hormonal equilibre establishment. | 1976 | 134817 |
| [sofan, a dmso-containing dermatologic agent in small-animal practice]. | | 1975 | 169806 |
| use of vidarabine in epidemic keratoconjunctivitis due to adenovirus types 3, 7, 8, and 19. | adenovirus types 3,7,8, and 19 were isolated during an outbreak of epidemic keratoconjunctivitis. there was no clinically recognizable difference in the severity of the disease produced by each adenovirus type. twenty-nine patients with positive viral cultures were given either 3.3% vidarabine ointment or polyvinyl alcohol eyedrops within five days of the onset od disease, and were observed for at least 14 days. an observer unaware of which medication was prescribed found subepithelial corneal i ... | 1976 | 187059 |
| metabolism of cyclohexane carboxylic acid by alcaligenes strain w1. | thirty-three microorganisms capable of growth with cyclohexane carboxylate as the sole source of carbon were isolated from mud, water, and soil samples from the aberystwyth area. preliminary screening and whole-cell oxidation studies suggested that, with one exception, all of the strains metabolized the growth substrate by beta-oxidation of the coenzyme a ester. this single distinctive strain, able to oxidize rapidly trans-4-hydroxycyclohexane carboxylate, 4-ketocyclohexane carboxylate, p-hydrox ... | 1978 | 207665 |
| modulation of noradrenergic transmission in the rabbit ear artery by dopamine. | 1. the effects of dopamine on vasoconstrictor responses to field stimulation of sympathetic nerves and to exogenous noradrenaline were studied in the isolated ear artery of the rabbit. responses to noradrenaline were unchanged at the start of the dopamine infusions but were enhanced as the infusions continued and also after cessation of the infustion. 2 dopamine (0.5 mum) reduced the stimulation-induced efflux of tritium from segments of ear artery labelled with [3h]-noradrenaline. the reduction ... | 1978 | 215260 |
| initiation of parturition in the human female. | | 1978 | 216122 |
| relationship between t-antigen and tumor-specific transplantation antigen in simian virus 40-transformed cells. | the simian virus 40 (sv40) tumor antigen (t-antigen) and tumor-specific transplantation antigen (tsta) have been partially purified and studied to clarify their relationship. the t-antigen and the tsta were partially purified from nuclei of sv al/n cells, and sv40-transformed mouse embryo fibroblast line, by precipitation with ammonium sulfate and chromatography on deae- and dna-cellulose. the t-antigen was assayed by complement fixation, and the tsta was assayed by its ability to immunize mice ... | 1979 | 219235 |
| herpes simplex virus type 2 functions expressed during stimulation of human cell dna synthesis. | experiments were designed to identify herpes simplex virus type 2 (hsv-2)-specific functions expressed during stimulation of human embryo fibroblast dna synthesis. cultures were partially arrested in dna synthesis by pretreatment with 5-fluorouracil and maintenance in low-serum (0.2%) medium during virus infection. results showed that continuous [methyl-(3)h]thymidine uptake into cellular dna was ninefold greater in hsv-2-infected than in mock-infected cultures measured after 24 h of incubation ... | 1979 | 219236 |
| studies on the neurobehavioral effects of polybrominated biphenyls in rats. | | 1979 | 222189 |
| akr leukemogenesis: identification and biological significance of thymic lymphoma receptors for akr retroviruses. | we have previously demonstrated that in vitro cell lines of mouse thymic lymphomas express surface receptors specific for the retrovirus that induced them. this study extends these observations to an analysis of receptor-bearing cells in the preleukemic and leukemic phases of spontaneous akr thymic lymphomagenesis. akr mice regularly begin expressing n-tropic retroviruses (as assayed on nih fibroblasts by the xc plaque assay) in several tissues early in life; thymic lymphocytes also express thes ... | 1979 | 222476 |
| e. coli dna binding protein hu forms nucleosomelike structure with circular double-stranded dna. | the incubation of the e coli dna binding protein hu with relaxed circular sv40 dna in the presence of pure nicking-closing enzyme introduces up to 18 negative superhelical turns in the dna molecules as measured by agarose gel electrophoresis. the maximal density of supercoiling is obtained at a hu-dna mass ratio of 1. reconstituted dna-hu complexes prefixed with glutaraldehyde appear as condensed circular structures having an average of 14 "beads" per circular sv40 dna molecule, with a "bead" di ... | 1979 | 222478 |
| [sensitivity of anaerobes in gyneco-obstetric infections]. | | 1978 | 232934 |
| proceedings: effect of catalin (1-hydroxy-(3,2alpha)-5-phenoxazone-3-carboxylic acid) on the reduced coenzymes nadh and nadph. | | 1975 | 235444 |
| tail bites dog. | | 1978 | 246554 |
| arteriovenous shunts demonstrated in the apical circulation of rat incisor teeth by the use of radio-labeled microspheres. | the distribution of systemically introduced 85sr-labeled tracer microspheres to unexposed and previously exposed rat lower incisor tooth pulps was investigated. fewer tracer microspheres entered the microcirculation of pulps that had been previously exposed as compared to control unexposed pulps. the presence of large numbers of dilated blood vessels in the apical tissues of teeth with exposed pulps was observed, and it is postulated that these vessels may be operating as arteriovenous anastomos ... | 1979 | 285404 |
| shapes of proteins l1, l9, l25, and l30 from the 50s subunit of the escherichia coli ribosome, determined by hydrodynamic studies. | proteins l1, l9, l25, and l30, purified by a nondenaturing method from the 50s ribosomal subunit of escherichia coli a19, have been characterized. the four proteins were studied under conditions which resemble those used for reconstitution experiments. these proteins have s020,w values of 2.0 s, 1.8 s, 1.8 s, and 1.0 s and d20,w values of 8.4 x 10(-7), 9.0 x 10(-7), 14.0 x 10(-7), and 15.0 x 10(-7) cm2/s. apparent specific volumes at 20 degrees c are 0.738, 0.733, 0.700, and 0.735 ml/g for the f ... | 1979 | 375974 |
| uptake of (methyl-14c)-sisomicin and (methyl-14c)-gentamicin into bacterial cells. | eight sensitive strains (two staphylococcus aureus, two escherichia coli, two pseudomonas aeruginosa and two klebsiella pneumoniae) and four resistant pseudomonas aeruginosa strains were used to study uptake of sisomicin and gentamicin by the bacterial cells. in eleven out of the twelve organisms studied employing (methyl-14c)-sisomicin and (methyl-14c)-gentamicin, uptake of the former was found higher that that of the latter. in one organism, the uptake of the two antibiotics was similar. this ... | 1978 | 416011 |
| effect of sodium hydroxide upon protein binding to filter paper disks and its significance in the determination of protein in colorimetric and radioisotopic assays. | | 1979 | 543545 |
| synchronization of the fission yeast schizosaccharomyces pombe using heat shocks. | | 1975 | 1196141 |
| [changes in the activity of succinate dehydrogenase in the cells of different brain structures under the effect of uhf field of low intensity]. | | 1976 | 1274298 |
| expression and release of neuroregulators during development: monoamines and neuropeptides. | the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ... | 1992 | 1306802 |
| inhibin-like "antigonadotropic" activity in the ovine pineal. | in an attempt to define the antigonadotropic activity associated with the protein/peptide extracts of ovine pineals, melatonin- and steroid-free fractions obtained after subjecting crude ovine pineal acetone powder to soft gel chromatography were tested in three bioassay systems: (1) the conventional compensatory ovarian hypertrophy inhibition assay in rats, (2) the coupled bioassay in immature mice that enables one to distinguish factors acting at the level of the pituitary from those acting at ... | 1992 | 1453311 |
| horn fly (diptera: muscidae) insecticide resistance in kentucky and arkansas. | the effect of previous insecticide use patterns for horn fly control on the susceptibility spectrum of horn fly (haematobia irritans [l.]) populations from kentucky and arkansas is described. populations of horn flies from both states were tested with three pyrethroids (cyhalothrin, cypermethrin, and permethrin), three organophosphates (diazinon, pirimiphos methyl, and tetrachlorvinphos), and a chlorinated hydrocarbon (methoxychlor). dose-mortality data indicated insecticide resistance in arkans ... | 1991 | 1885841 |
| diabetes-induced rat hyposensitivity to compound 48/80. | rat mortality and contractile responses of isolated tracheas to compound 48/80 from rats made diabetic 4 days before by a single intravenous injection of alloxan and from diabetic rats that had been treated with insulin 6 h before were compared with control animals. diabetic animals and tracheal segments from diabetic rats were significantly less responsive to compound 48/80 than control and insulin-treated diabetic animals. on the other hand, diabetic animals have a lower quantity of peritoneal ... | 1991 | 1913334 |
| [postcoital uterine microbe colonization and endometritis in the mare]. | in the mare, natural breeding is associated with bacterial contamination of the reproductive tract. the purpose of this study was to examine postcoital bacterial contamination and the resulting inflammatory response of the uterus. uterine swabs for bacteriological and cytological examination were obtained from 80 mares. each mare was sampled once between 4 and 69 hours postbreeding. in mares which did not conceive, sampling was repeated at the following estrus. the findings were compared with th ... | 1991 | 1948985 |
| protective effect of inactivated pasteurella haemolytica bacterin challenged in bovine herpesvirus-1 experimentally infected calves. | formalin-inactivated and sonicated pasteurella haemolytica bacterins were prepared from 1 h cultures of bacterial pellets in rpmi-1640 medium containing 7% fetal calf serum. the bacterial pellets were obtained from logarithmic phase growth of the organism by centrifugation. the protective effect of the vaccine was evaluated in 43 specific-pathogen-free hereford crossbred calves and yearlings in three experiments. cattle were either single vaccinated or boosted via three routes; intratracheally ( ... | 1990 | 2168606 |
| phosphonylmethyl ethers of acyclic nucleoside analogues: inhibitors of hsv-1 induced ribonucleotide reductase. | diphosphates of n-(s)-(3-hydroxy-2-phosphonylmethoxypropyl) and n-(2-phosphonylmethoxyethyl) derivatives of purine and pyrimidine heterocyclic bases inhibit hsv-1 encoded ribonucleotide reductase. of the compounds studied, the most efficient inhibitors of cdp reduction (at 5.1 mumols.l-1) by the hsv-1-encoded enzyme are hpmpapp (ic50 = 0.9 mumols.l-1) and pmeapp (ic50 = 8 mumol.l-1). pmeapp does not inhibit the enzyme isolated from the mutant hsv-1 kos strain pmear which is resistant to pmea at ... | 1990 | 2168691 |
| nitrate reductases of escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narghji operon. | the structural genes for nrz, the second nitrate reductase of escherichia coli, have been sequenced. they are organized in a transcription unit, narzywv, encoding four subunits, narz, nary, narw and narv. the transcription unit is homologous (73% identity) to the narghji operon which encodes the genes for nra, the better characterized nitrate reductase of this organism. the level of homology between the corresponding polypeptides ranges from 69% for the narw/narj pair to 86% for the narv/nari pa ... | 1990 | 2233673 |
| [effect of enalapril on heart rate, arterial blood pressure and exocrine pancreatic secretion in the alert dog]. | a changed exocrine pancreatic secretion could be a pathogenetic factor of an acute pancreatitis after administration of angiotensin-converting enzyme (ace) inhibitors. in six conscious dogs with gastric and duodenal thomas fistulas we studied the effect of an intravenous (iv.) bolus injection of 10 mg enalaprilat, an intraduodenal (id.) bolus injection of 20 and 40 mg enalapril (e.), and 0.15 m nacl (20 ml iv., resp., id.) on pancreatic bicarbonate- and protein output in response to secretin (20 ... | 1990 | 2238764 |
| [quantitative characteristics of microcirculation bed in aseptic inflammation of the rat uterus and with the background of the use of carbon-mineral sorbents]. | we studied the volume compactness of the arterial, capillary and venous parts of the microcirculation channel after 24, 48, 72 and 96 hours of the aseptic inflammation and after local application of the carbon-mineral sorbents in the rat's uterus. the local sorption decreased the reaction of the inflammation in the endo- and myometrium. | 1990 | 2279103 |
| effects of human pth-related peptide and human pth on cyclic amp production and cytosolic free calcium in an osteoblastic cell clone. | recently, human parathyroid hormone-related peptide (hpthrp) has been purified and its amino acid sequence determined. within the amino-terminal 13 residues of hpthrp, 8 amino acids were found homologous between hpthrp and human pth (hpth). this peptide was reported to stimulate cyclic amp (camp) production in osteoblastic cell lines (umr106 and ros17/2.8). however, whether or not this peptide affects another second messenger, i.e., cytosolic free calcium ([ca2+]i), in osteoblasts has not yet be ... | 1989 | 2546638 |
| characterization of the rela1 mutation and a comparison of rela1 with new rela null alleles in escherichia coli. | the most widely studied "relaxed" mutant of the rela locus, the rela1 allele, is shown here to consist of an is2 insertion between the 85th and 86th codons of the otherwise wild-type rela structural gene, which normally encodes a 743-amino acid (84 kda) protein. the rela protein is a ribosome-dependent atp:gtp (gdp) pyrophosphoryltransferase that is activated during the stringent response to amino acid starvation and thereby occasions the accumulation of guanosine 3',5'-bispyrophosphate (ppgpp). ... | 1989 | 2556396 |
| attenuation of defensive threat and attack in wild rats (rattus rattus) by benzodiazepines. | a battery of tests designed to elicit reactions to a variety of non-painful threat stimuli was used to study the effects of chlordiazepoxide (5-20 mg/kg), diazepam (1-5 mg/kg) and midazolam (1-10 mg/kg) on the defensive repertoire of wild rattus rattus. the most consistent effect of benzodiazepine treatment, across compounds and tests, was a marked reduction in defensive threat and attack behaviors, with midazolam effective over a wider range of situations. in contrast, effects on freezing and f ... | 1989 | 2566187 |
| partial coexistence of growth hormone-releasing hormone and tyrosine hydroxylase in paraventricular neurons in rats. | immunocytochemistry revealed growth hormone-releasing hormone (grf)-containing cells (4-10/50 microns thick coronal sections) in the ventral portion of the medial parvicellular subdivision of the paraventricular nucleus (pvn) in the rat. in the same region we also detected about the same number of neurons containing the mrna that encodes grf using in situ hybridization histochemistry. fluorescence double labelling immunohistochemistry showed the presence of grf and tyrosine hydroxylase (th) in t ... | 1989 | 2573894 |
| cytotoxicity and effect on mutagenicity of buffers in a microsuspension assay. | we have examined the effects of three buffers--vogel-bonner minimal e (vbm) and 0.15 and 0.015 m sodium phosphate, ph 7.4--and two concentrations of overnight cells (5x and 10x) on the mutagenicity of two pure compounds and two complex mixtures in strain ta98 using a modification of a microsuspension assay developed by kado et al. (mutation research 121:25-32, 1983). the assay was performed by adding 50 microliters of cell concentrate of an overnight culture of ta98 resuspended in the appropriat ... | 1989 | 2575290 |
| phosphoroselenoate oligodeoxynucleotides: synthesis, physico-chemical characterization, anti-sense inhibitory properties and anti-hiv activity. | oligodeoxynucleotides with a phosphorus atom in which one of the non-bridging oxygen atoms is substituted by selenium were prepared and investigated with respect to their antisense properties. a general synthesis of phosphoroselenoate analogs of oligonucleotides is described using potassium selenocyanate as the selenium donor. the compounds, characterized by 31p nmr, were shown to decompose to phosphate with a half-life of ca. 30 days. melting temperatures of duplexes between poly(ra) or poly(ri ... | 1989 | 2682524 |
| fever and urticaria in acute giardiasis. | travelers' diarrhea afflicts some 250 million people yearly. a number of etiologic agents have been identified, including bacteria, viruses, and parasites. giardia lamblia is one of the pathogens clearly associated with this syndrome. typical symptoms of giardiasis that include abdominal bloating and cramps are well known, whereas urticaria has rarely been associated with this illness. an american tourist developed acute giardiasis accompanied by urticaria and high fever. no other pathogens were ... | 1989 | 2705846 |
| in vivo dna cross-linking by cyclophosphamide: comparison of human chronic lymphatic leukemia cells with mouse l1210 leukemia and normal bone marrow cells. | alkaline elution was done on a variety of cells following cyclophosphamide (cy) treatment in vivo. cells used were l1210 leukemia, normal mouse bone marrow, and peripheral blood cells obtained from a patient with chronic lymphatic leukemia (cll). endpoints used were determination of single strand breaks, dna-dna interstrand and dna-protein cross-links. after treatment of mice with cy (4 mg/mouse), low levels of single strand breaks were observed in both l1210 leukemia and cdf1 normal bone marrow ... | 1989 | 2731167 |
| accurate and efficient rna polymerase iii transcription in a cell-free extract prepared from ascaris suum embryos. | high-speed supernatant (s100) extracts derived from homogenized ascaris suum embryos efficiently transcribe added rna polymerase iii templates including cloned 5s rrna genes of the filarial parasite brugia malayi. several criteria, including two-dimensional rnase t1 oligonucleotide fingerprint analysis, indicate that in vitro transcription is accurately initiated and terminated. | 1989 | 2747746 |
| the influence of endogenous and exogenous factors on the course and the results of experimental work with human tumor tissue on athymic nude rodents. | | 1989 | 2749469 |
| twenty-year comparison of the human allograft and porcine xenograft. | this study compares a retrospective consecutive series of human allografts and concurrent porcine xenografts implanted over a 10-year interval. there were 571 allograft valves and 1,351 xenograft valves implanted in the aortic and mitral position with mean follow-up of 12.8 years for the allografts and 6.2 years for the xenografts. this study compares the incidence of structural deterioration over long-term follow-up. we found that there was no significant difference between four manufacturers o ... | 1989 | 2774760 |
| ontogeny of androgen receptors in fetal guinea pig brain. | sexual differentiation of the guinea pig brain is androgen dependent. to understand the cellular mechanisms of androgen action, we studied the ontogeny of cytosolic (arc) and nuclear (arn) androgen receptors in the brains and anterior pituitaries of fetal, neonatal, and adult guinea pigs. using cytosol from the hypothalamus-preoptic area-amygdala-septum of 60- to 65-day fetuses and nuclear preparations from 6-day-old neonates treated with testosterone propionate, validation studies revealed an a ... | 1989 | 2804213 |
| ontogeny of androgen receptors in fetal guinea pig brain. | sexual differentiation of the guinea pig brain is androgen dependent. to understand the cellular mechanisms of androgen action, we studied the ontogeny of cytosolic (arc) and nuclear (arn) androgen receptors in the brains and anterior pituitaries of fetal, neonatal, and adult guinea pigs. using cytosol from the hypothalamus-preoptic area-amygdala-septum of 60- to 65-day fetuses and nuclear preparations from 6-day-old neonates treated with testosterone propionate, validation studies revealed an a ... | 1989 | 2804213 |
| obstetrical medication and the human newborn: the influence of alphaprodine hydrochloride on visual behaviour. | the visual habituation of two groups of newborn infants was tested at a mean age of 2.9 days with large achromatic squares of varying luminance. mothers of the infants in one group received no medication during labour; mothers in the second group received a single dose of the synthetic narcotic alphaprodine hydrochloride. the two groups did not differ in the number of trials to reach the criterion, but newborns in the no-medication group fixated the stimuli for much longer periods than did infan ... | 1989 | 2806744 |
| formation of mboii vectors and cassettes using asymmetric mboii linkers. | class-iis restriction endonucleases such as mboii cleave dna at a specified distance away from their recognition sequences. this feature was exploited to cleave dna at previously inaccessible locations by preparing special asymmetric linker/adapters containing the mboii recognition sequence. these could be joined to dna fragments and subsequently cleaved by mboii. attachment of a 3' phosphate to one of the two different oligodeoxynucleotides comprising the asymmetric duplex prevented ligation at ... | 1987 | 2820843 |
| formation of mboii vectors and cassettes using asymmetric mboii linkers. | class-iis restriction endonucleases such as mboii cleave dna at a specified distance away from their recognition sequences. this feature was exploited to cleave dna at previously inaccessible locations by preparing special asymmetric linker/adapters containing the mboii recognition sequence. these could be joined to dna fragments and subsequently cleaved by mboii. attachment of a 3' phosphate to one of the two different oligodeoxynucleotides comprising the asymmetric duplex prevented ligation at ... | 1987 | 2820843 |
| regulatory functions of protein phosphorylation in meiotic maturation of mouse oocytes in vitro. | | 1988 | 2853366 |
| regulatory functions of protein phosphorylation in meiotic maturation of mouse oocytes in vitro. | | 1988 | 2853366 |
| a novel peptide vasoconstrictor, endothelin, is produced by vascular endothelium and modulates smooth muscle ca2+ channels. | since the discovery of endothelium-dependent vasodilation, vascular endothelium has been recognized as an important functional unit contributing to the regulation of vascular tonus. recent purification, structure determination and molecular cloning of a novel peptidergic vasoconstrictor, endothelin, from the culture supernatant of porcine aortic endothelial cells has further substantiated this concept. starting from a large quantity of serum-fee endothelial cell-conditioned medium, endothelin wa ... | 1988 | 2853725 |
| development of udp-glucuronosyltransferase activity toward digitoxigenin-monodigitoxoside in neonatal rats. | glucuronidation is low or undetectable in embryonic and early fetal tissues and changes to adult levels at rates depending on the acceptor, tissue, and species. because other data indicate there may be a specific udp-glucuronosyltransferase (gt) in the liver of adult rats that glucuronidates digitoxigenin-monodigitoxoside (dig), the development of gt activity in neonatal rats toward dig was compared with that of other acceptors. conjugation of p-nitrophenol and 1-naphthol was higher at birth and ... | 1985 | 2859166 |
| development of udp-glucuronosyltransferase activity toward digitoxigenin-monodigitoxoside in neonatal rats. | glucuronidation is low or undetectable in embryonic and early fetal tissues and changes to adult levels at rates depending on the acceptor, tissue, and species. because other data indicate there may be a specific udp-glucuronosyltransferase (gt) in the liver of adult rats that glucuronidates digitoxigenin-monodigitoxoside (dig), the development of gt activity in neonatal rats toward dig was compared with that of other acceptors. conjugation of p-nitrophenol and 1-naphthol was higher at birth and ... | 1985 | 2859166 |
| [criteria of the reactions of immature nerve tissues to oxygen deficiency and possibilities of its control]. | | 1985 | 2863001 |
| analgesic effectiveness of topically applied dynorphin on human dental pulp. | | 1986 | 2876436 |
| the ascending projections of the dopaminergic neurons of the substantia nigra, zona reticulata: a combined retrograde tracer-immunohistochemical study. | the efferent projections of the dopaminergic neurons in the zona reticulata of the substantia nigra were examined using a combined retrograde tracer-immunohistochemical method. these dopamine (da) neurons were found to project exclusively to striatal targets in a topographically defined fashion. the zona reticulata da neurons do not innervate mesolimbic or mesocortical dopaminergic terminal fields, nor do they project to the superior colliculus or the ventromedial thalamic nucleus. these data su ... | 1986 | 2879262 |
| frequency of fimbriation of nontypable haemophilus influenzae and its ability to adhere to chinchilla and human respiratory epithelium. | to date, we have examined nearly 60 clinical isolates of nontypable haemophilus influenzae (26 nasopharyngeal, 33 from middle ear effusions) and have found that 100% were fimbriated. the percentage of cells bearing fimbriae within each isolate varied from less than 10 to 100%, with fimbriae being either peritrichous or bipolar in distribution. fimbriae were approximately 2.4 to 3.6 nm in width; however, there was a high degree of variability in both length and number of fimbriae per individual b ... | 1988 | 2892792 |
| comparison of adrenal medullary, carotid body and pc12 cell grafts in 6-ohda lesioned rats. | the survival and functional properties of dispersed cell implants of catecholaminergic cells obtained from the peripheral nervous system of adult rats (adrenal medulla and carotid body glomus cells) and pc12 cells from a rat pheochromocytoma cell line were examined following transplantation into the striatum of the adult rat. the host animals, all with unilateral 6-hydroxydopamine (6-ohda) nigrostriatal lesions, were divided into 5 groups: (1) pc12 cells transplanted into cyclosporin-a treated h ... | 1988 | 2896533 |
| endocytosis of proteoheparan sulfate by cultured skin fibroblasts. | endocytosis by cultured human skin fibroblasts of 35so4(2-)-labeled proteoheparan sulfate from fibroblast secretions was quantitatively investigated. at a constant dose of proteoheparan sulfate, uptake and subsequent intracellular degradation but not binding to the cell membrane increased with time. compared with the endocytosis of total secreted proteoglycans containing predominantly a small proteodermatan sulfate, uptake of proteoheparan sulfate was 5- to 20-fold lower. nevertheless, uptake of ... | 1986 | 2943303 |
| morphine or capsaicin administration alters the secretion of beta-endorphin into the hypophysial portal vasculature of the rat. | immunoreactive beta-endorphin (ir-beta-end) concentrations were measured in the hypophysial portal plasma of the male rat under urethane anesthesia. on the basis of immunochemical studies and gel filtration chromatography it appears that ir-beta-end in rat hypophysial portal plasma is primarily beta-endorphin (beta-end) and not beta-lipotropin (beta-lph). in addition, much of the ir-beta-end in portal plasma may be of pituitary origin since acute hypophysectomy resulted in approximately an 80% d ... | 1986 | 2944026 |
| human milk-derived lactoferrin inhibits mitogen and alloantigen induced human lymphocyte proliferation. | purified lactoferrin (lf), isolated from human milk, was tested for its effect on human t-lymphocyte proliferative responses to phytohemagglutinin (pha) and to alloantigens in mixed lymphocyte culture. lf inhibited proliferation in both assays in a dose-dependent manner. the suppressive effect was not due to lf mediated cytotoxicity since washing cells that had been pre-incubated with lf restored their proliferative activity. lf was most effective in suppressing the pha response when added withi ... | 1987 | 2963123 |
| demonstration of a ouabainlike plasma compound in hypertension prone and hypertension resistant rats. | material extracted and partially purified from plasma of the sabra hypertension prone rats was found to be capable of 1) inhibiting the binding of 3h-ouabain to rat brain synaptosomes, 2) inhibiting the activity of rat brain microsomal na, k activated adenosine triphosphatase, and 3) increasing the contractile force of rat heart muscle. the results demonstrate the presence of a ouabainlike compound in the plasma of these rats. the plasma concentration of this compound in sabra hypertension prone ... | 1985 | 2993163 |
| secretion of anti-müllerian hormone by immature bovine sertoli cells in primary culture, studied by a competition-type radioimmunoassay: lack of modulation by either fsh or testosterone. | secretion of anti-müllerian hormone (amh) by immature bovine sertoli cells in primary culture was studied through a competition-type ria employing a polyclonal antibody and 125i-labelled purified amh. this ria is approximately 10 times more sensitive than the solid-phase two-site monoclonal antibody-based ria described previously. biosynthesis and secretion of amh by cultured sertoli cells require approximately 48 h, are not influenced by fsh or testosterone and steadily decrease over a one-week ... | 1985 | 3000849 |
| the near infrared (nir) absorption band of cytochrome aa3 in purified enzyme, isolated mitochondria and in the intact brain in situ. | | 1985 | 3008514 |
| synthesis of eicosanoids by tissues of the synovial joint during the development of chronic erosive synovitis. | | 1986 | 3008530 |
| markers for estimating digesta flow in pigs and the effects of dietary fiber. | effects of diluting the energy content of a corn-soybean meal diet with either alfalfa meal or corn cobs on nutrient digestibility and rate of passage of feed residues and particle markers were measured in crossbred (yorkshire x landrace x chester white x large white) barrows with a mean body weight of about 80 kg. the excretion pattern in the feces of cr-mordanted diet and of rare earths initially bound individually to the mixed diet or to the corn or soybean meal suggested a model having a sin ... | 1986 | 3021704 |
| markers for estimating digesta flow in pigs and the effects of dietary fiber. | effects of diluting the energy content of a corn-soybean meal diet with either alfalfa meal or corn cobs on nutrient digestibility and rate of passage of feed residues and particle markers were measured in crossbred (yorkshire x landrace x chester white x large white) barrows with a mean body weight of about 80 kg. the excretion pattern in the feces of cr-mordanted diet and of rare earths initially bound individually to the mixed diet or to the corn or soybean meal suggested a model having a sin ... | 1986 | 3021704 |
| pain, increasing girth in healthy japanese man. | | 1986 | 3021791 |
| pain, increasing girth in healthy japanese man. | | 1986 | 3021791 |
| diabetic embryopathy and fuel-mediated organ teratogenesis: lessons from animal models. | the growing recognition that faulty maternal metabolism during early organogenesis may be implicated in the increased incidence of birth defects in pregnancies complicated by diabetes has prompted worldwide efforts to institute improved preconceptional metabolic regulation. however, the failure to identify the periods of greatest risk for diabetic embryopathy, the mediating teratogen(s), and the underlying mechanisms have complicated attempts to establish precise therapeutic guidelines and targe ... | 1988 | 3053387 |
| isolation of enzymes with novel capabilities for use in clinical analysis. | | 1988 | 3067633 |
| isolation of enzymes with novel capabilities for use in clinical analysis. | | 1988 | 3067633 |
| chronic cardiac toxicity after inhalation of 1,1,1-trichloroethane. | two patients showed evidence of chronic cardiac toxicity after repeated exposure to 1,1,1-trichloroethane. in both cases there was circumstantial evidence of a deterioration after routine anaesthetic use of the related compound halothane. an adolescent boy who sniffed trichloroethane presented with multiple ventricular arrhythmias during tonsillectomy. follow up showed mild chronic left ventricular impairment. a 54 year old man had repeated industrial exposure to trichloroethane and deteriorated ... | 1987 | 3105712 |
| chronic cardiac toxicity after inhalation of 1,1,1-trichloroethane. | two patients showed evidence of chronic cardiac toxicity after repeated exposure to 1,1,1-trichloroethane. in both cases there was circumstantial evidence of a deterioration after routine anaesthetic use of the related compound halothane. an adolescent boy who sniffed trichloroethane presented with multiple ventricular arrhythmias during tonsillectomy. follow up showed mild chronic left ventricular impairment. a 54 year old man had repeated industrial exposure to trichloroethane and deteriorated ... | 1987 | 3105712 |
| [experimental infection of sarcocystis suihominis in pigs]. | | 1987 | 3113762 |
| prostacyclin biosynthesis and phospholipase activity in hypoxic rat myocardium. | phospholipid metabolism was studied in rat myocardial slices that were incubated under normoxia or hypoxia for up to 24 hours. phospholipid degradation was prominent in hypoxic myocardium, particularly phosphatidylcholine, which markedly decreased after 24 hours of hypoxia. in contrast, lysophosphatidylcholine increased. the mechanism of phospholipid degradation in hypoxic myocardium was studied. the highest activity for phospholipase a2 among subcellular fractions was found in microsomal fracti ... | 1988 | 3133132 |
| interaction of acidic isoferritins with human promyelocytic hl60 cells. | we have used undifferentiated human promyelocytic hl60 cells to study the binding of radioiodinated human ferritin in vitro. specific binding of human heart ferritin could be demonstrated at 37 degrees c, whereas no binding of liver ferritin could be found. the uptake of labelled heart ferritin was abolished by incubation at 4 degrees c, by prior treatment of the hl60 cells with pronase and by the addition of human plasma to the medium. on the other hand, the addition of excess unlabelled human ... | 1988 | 3165673 |
| blastocystis hominis: clinical and therapeutic aspects. | | 1988 | 3176148 |
| the effect of alcoholic beverages on iron and zinc metabolism in the rat. | 1. male wistar rats (approximately 200 g) were given distilled water and a semi-synthetic control diet for 6 d. on day 7, 37 kbq 65zn were administered intramuscularly and the rats were given distilled water, beer, cider, red wine, whisky or ethanol as their only source of fluid. the wine, whisky and ethanol were diluted so that each of the beverages contained a similar ethanol concentration (approximately 30 g/l). food and fluid intake, growth rate and whole-body 65zn were measured regularly ov ... | 1988 | 3196669 |
| characterization of an aeromonas hydrophila strain isolated on a septicemic out-break in a fish-farm of spain. | an aeromonas hydrophila strain (ah-3) was isolated from a septicemic out-break on a gold-fish farm near barcelona (spain). on the bases of their virulence and surface characteristics was classified as moderate to weakly virulent. | 1988 | 3271064 |
| ethical considerations of the new reproductive technologies. by the ethics committee (1986-87) of the american fertility society in light of instruction on the respect for human life in its origin and on the dignity of procreation issued by the congregation for the doctrine of the faith. | in september 1986, the american fertility society issued a report, ethical considerations of the new reproductive technologies, setting forth the then-held ethical position of the society on the various new reproductive technologies. in 1987, the congregation for the doctrine of the faith issued the instruction on the respect for human life and its origin and on the dignity of procreation. while both documents state that very similar moral criteria were used to derive ethical positions with resp ... | 1988 | 3276566 |
| in vivo inorganic chemistry of technetium cations. | technetium-99m cations are of interest because of their potential use as myocardial perfusion imaging agents. these species can undergo in vivo reactions which markedly affect their biodistribution patterns. four such cases of reactions are presented and discussed: (1) simple ligand substitution; (2) metal centered redox processes; (3) reactions of coordinated ligands; (4) outer sphere association reactions. new experimental techniques appropriate for investigating these reactions at the 10(-10) ... | 1987 | 3305808 |
| protective effects of the thromboxane receptor antagonists bm 13.177 and bm 13.505 against u 46619-induced sudden death in rats. | the purpose of this study was to evaluate the pharmacology and pharmacodynamics of the thromboxane receptor antagonists, bm 13.177 and bm 13.505, for prevention of u 46619-induced sudden death in anesthetized male sprague-dawley rats. the injection of u 46619 (100 micrograms/kg i.v.) produced sudden death typically between 5 and 15 min. administration of 0.01 mg/kg bm 13.505 (i.v.) 0.1 h prior to the u 46619 challenge did not protect against sudden death, while doses of 0.03 mg/kg or greater pro ... | 1988 | 3406046 |
| control of heme synthesis during friend cell differentiation: role of iron and transferrin. | in many types of cells the synthesis of delta-aminolevulinic acid (ala) limits the rate of heme formation. however, results from our laboratory with reticulocytes suggest that the rate of iron uptake from transferrin (tf), rather than ala synthase activity, limits the rate of heme synthesis in erythroid cells. to determine whether changes occur in iron metabolism and the control of heme synthesis during erythroid cell development friend erythroleukemia cells induced to erythroid differentiation ... | 1986 | 3464611 |
| activity of a partially purified human bcgf on murine assays for b-cell stimulatory factors. i. bcgf ii-like activity of human bcgf. | to further characterize a human b-cell growth factor (bcgf) produced by phytohemagglutinin (pha) p-stimulated peripheral blood t cells, a partially purified preparation of this material was tested in a number of murine assays for b-cell stimulatory factors (bsf). human bcgf lacked murine bsf-1 activity as assessed via the induction of polyclonal proliferation of anti-igm-stimulated murine b cells; however, this material consistently augmented the proliferative response of murine b cells to anti- ... | 1987 | 3499989 |
| reversible and irreversible neuronal damage caused by excitatory amino acid analogues in rat cerebellar slices. | slice preparations of the developing rat cerebellum were used to investigate the light and electron microscopic correlates of reversible and irreversible neuronal injury caused by the neurotoxic excitatory amino acid receptor agonists, kainate and n-methyl-d-aspartate. the slices were examined after various periods of exposure to the agonists (up to 30 min) with or without a 90 min recovery period in agonist-free medium. n-methyl-d-aspartate (100 microm) caused necrosis of deep nuclear neurons a ... | 1986 | 3526173 |
| reversible and irreversible neuronal damage caused by excitatory amino acid analogues in rat cerebellar slices. | slice preparations of the developing rat cerebellum were used to investigate the light and electron microscopic correlates of reversible and irreversible neuronal injury caused by the neurotoxic excitatory amino acid receptor agonists, kainate and n-methyl-d-aspartate. the slices were examined after various periods of exposure to the agonists (up to 30 min) with or without a 90 min recovery period in agonist-free medium. n-methyl-d-aspartate (100 microm) caused necrosis of deep nuclear neurons a ... | 1986 | 3526173 |
| pulsatile release of antipyretic neuropeptide alpha-msh from septum of rabbit during fever. | the effects on fever of central administration of alpha-melanocyte-stimulating hormone (alpha-msh) and its antiserum, the specific increase in central concentration of alpha-msh during fever, and related results indicate that this neuropeptide is important to central nervous system (cns) regulation of the febrile response. the present experiments were designed to establish whether alpha-msh is released from septal tissue during fever and, if so, in what temporal course relative to the febrile re ... | 1987 | 3591986 |
| the binding of decomposition products of udp-galactose to the microsomes and polyribosomes isolated from rat liver. | udp-d-[u-14c]galactose is decomposed to [u-14c]galactose-1-phosphate and [u-14c]galactose by rat liver microsomal and crude polyribosomal fractions, under conditions commonly used to assay of glycosyltransferase activities. udp-d-[u-14c]galactose, at neutral ph, is also chemically degraded to the [u-14c]galactose-1,2-cyclic phosphate. the 1,2-cyclic phosphate derivative of galactose also exists in the commercial udp-d-[u-14c]galactose. it is a very important finding that products of the udp-d-[u ... | 1987 | 3675917 |
| non-invasive estimation of afferent inputs for postural stability under low levels of alcohol. | the maintenance of human equilibrium is a complex process. several physiological systems provide afferent information for postural stabilization. this study describes a noninvasive methodology to estimate the relative roles of interacting afferent inputs relevant for postural control. a multiaxis force platform in conjunction with specially designed test procedure provided semi-quantitative insight into the roles of vision, proprioceptors and vestibular apparatus in controlling postural sway whe ... | 1987 | 3688583 |
| neurotropin increases in vitro life span of human fibroblasts. | fibroblasts isolated from human fetal tissues (skin, lung and heart) exhibited the following population doubling levels (pdl): about 40 pdl for skin; 60 pdl for lung; 10 pdl for heart. neurotropin extract from vaccinia-virus infected skin tissues of rabbits increases the growth rate of the fibroblast when added to the old lung cell culture (pdl 40, 70% of the maximum life span) but not when added to the young lung (pdl 5, 8% of the maximum life span). neurotropin (40 or 80 micrograms/ml) increas ... | 1986 | 3736135 |
| female urinary chemosignals influence scent-marking behavior in male mongolian gerbils (meriones unguiculatus). | the influence of females on the amount of scent-marking behavior displayed by male mongolian gerbils was investigated. males isolated from females scent mark at a low level which increases more than two-fold if females are present in the room for three weeks without direct contact with the males. a similar increase is obtained by application of pooled female urine directly onto the males' noses. | 1985 | 3832671 |
| chemotactic activity of hydroxyalkenals on rat neutrophils. | previous results from our laboratory showed that in vitro 4-hydroxyoctenal, 4-hydroxynonenal (hne) and 4-hydroxytetradecenal elicit a neutrophil chemotactic response in the presence of albumin. data presented here indicate that other hydroxyalkenals (4-hydroxyundecenal and 4-hydroxy-pentadecenal) also exert a chemotactic attraction on rat pleural neutrophils. the different aldehydes tested show a wide activity-concentration range, the most powerful being 4-hydroxyoctenal. unlike most chemoattrac ... | 1985 | 3839769 |
| [synthesis of vitamin d in the skin]. | | 1985 | 3898049 |
| immunology and immunopathology of malignant catarrhal fever. | | 1985 | 3917220 |
| monoclonal antibodies against mouse gamma-interferon inhibit tumoricidal macrophage activation by t lymphocytes. | a monoclonal antibody, an-18.17.24, specific for murine interferon-gamma (ifn-gamma) was produced by immunizing wistar rats with ifn-gamma secreted by a t-cell lymphoma, l12-r4, upon stimulation with phorbol myristic acetate (pma). antiviral activity as well as tumoricidal activation induced by pma-stimulated l12-r4 cell supernatant or by con a-stimulated normal spleen cells were neutralized at the same extent by an-18 monoclonal antibody. moreover, depletion experiments showed that inhibition o ... | 1985 | 3930308 |