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an evaluation of three new commercial peptones used in some nutrient media designed for the isolation and characterization of pathogenic vibrios. 1979262509
long-term activation of protein kinase c by nicotine in bovine adrenal chromaffin cells.previous results from our laboratory suggest that long-term treatment of primary cultured bovine adrenal medullary (bam) chromaffin cells with nicotine or phorbol 12-myristate 13-acetate, either of which directly activates protein kinase c (pkc), increases the mrna levels encoding catecholamine-synthesizing enzymes and proenkephalin. in the present study, we have examined the effects of nicotine on bam cell pkc activity with special emphasis on long-term effects. nicotine increased particulate p ...19921560224
long-term activation of protein kinase c by nicotine in bovine adrenal chromaffin cells.previous results from our laboratory suggest that long-term treatment of primary cultured bovine adrenal medullary (bam) chromaffin cells with nicotine or phorbol 12-myristate 13-acetate, either of which directly activates protein kinase c (pkc), increases the mrna levels encoding catecholamine-synthesizing enzymes and proenkephalin. in the present study, we have examined the effects of nicotine on bam cell pkc activity with special emphasis on long-term effects. nicotine increased particulate p ...19921560224
protease composition of exocytosed human skin mast cell protease-proteoglycan complexes. tryptase resides in a complex distinct from chymase and carboxypeptidase.we characterized the release and the protease composition of high m.w. complexes released from dispersed human skin mast cells, under conditions that did not disrupt the binding of proteases to proteoglycan. the net percent release ratio of tryptase to histamine, after anti-ige and calcium ionophore a23187 stimulation was higher than those for chymase or carboxypeptidase. this was explained by the greater cell association of carboxypeptidase and chymase, compared with tryptase, after mast cell d ...19921560203
protease composition of exocytosed human skin mast cell protease-proteoglycan complexes. tryptase resides in a complex distinct from chymase and carboxypeptidase.we characterized the release and the protease composition of high m.w. complexes released from dispersed human skin mast cells, under conditions that did not disrupt the binding of proteases to proteoglycan. the net percent release ratio of tryptase to histamine, after anti-ige and calcium ionophore a23187 stimulation was higher than those for chymase or carboxypeptidase. this was explained by the greater cell association of carboxypeptidase and chymase, compared with tryptase, after mast cell d ...19921560203
genetic mapping of the human tryptophan hydroxylase gene on chromosome 11, using an intronic conformational polymorphism.the identification of polymorphic alleles at loci coding for functional genes is crucial for genetic association and linkage studies. since the tryptophan hydroxylase (tph) gene codes for the rate-limiting enzyme in the biosynthesis of the neurotransmitter serotonin, it would be advantageous to identify a polymorphism in this gene. by examining introns of the human tph gene by pcr amplification and analysis by the single-strand conformational polymorphism (sscp) technique, an sscp was revealed w ...19921463016
structure and function of the cd16:zeta:gamma complex expressed on human natural-killer cells.natural-killer (nk) cells are large granular lymphocytes involved in host defense against tumor cells and virally-infected cells. in addition to natural cytotoxicity, nk cells can effect antibody-dependent cytotoxicity mediated by cd16 (fc gamma riiia alpha). it has recently been shown that cd16 is associated with disulfide-linked dimers composed of 2 homologous sub-units, zeta and gamma. these transducing molecules are also associated with other multimeric cell-surface receptors such as the t-c ...19921428397
guinea pig transfer factor: restriction of macrophage migration inhibitory activity to extraordinary dilution confirmed with demonstration of diminished potency of stored preparations. 197988292
[infection by human immunodeficiency virus and rheumatic diseases]. 19921569794
establishment of stable, cell-mediated immunity that makes "susceptible" mice resistant to leishmania major.cell-mediated, but not antibody-mediated, immune responses protect humans against certain pathogens that produce chronic diseases such as leishmaniasis. effective vaccination against such pathogens must therefore produce an immunological "imprint" so that stable, cell-mediated immunity is induced in all individuals after natural infection. balb/c mice "innately susceptible" to leishmania major produce antibodies after substantial infection. in the present study, "susceptible" mice injected with ...19921636090
masseter spasm in children and pigs. 19921489378
re: endoscopic injection of glutaraldehyde cross-linked bovine dermal collagen for correction of vesicoureteral reflux. 19921472245
time course of cerebral blood flow and histological outcome after focal cerebral ischemia in rats.the relation between time-dependent changes in cerebral blood flow and the appearance of infarction after focal cerebral ischemia is still a matter for debate. the aim of this study was to measure perfusion after simultaneous occlusions of the left middle cerebral artery and ipsilateral common carotid artery in rats and correlate it with the timing and distribution of histological changes.19921636189
development of a competitive radioimmunoassay and a sandwich enzyme-linked immunosorbent assay for recombinant human granulocyte colony-stimulating factor. application to a pharmacokinetic study in rats.a competitive radioimmunoassay (ria) and a sandwich enzyme-linked immunosorbent assay (elisa) for the measurement of recombinant human granulocyte colony-stimulating factor (rhg-csf) in rat serum was developed using polyclonal antibody obtained by immunizing rabbits with rhg-csf. the anti-rhg-csf antibody did not recognize other colony-stimulating factors or interleukins. ria and elisa gave satisfactory reproducibility as judged by intra- and inter-assay precision. good agreements between the ri ...19921280299
the effect of magnesium deficiency on serum aldosterone in rats fed two levels of sodium. 19751196006
the ultrastructure of the rickettsia-like microorganism wolbachia pipientis and associated virus-like bodies in the mosquito culex pipiens. 1978671578
[intravenous cholangiography in hyperbilirubinaemia (author's transl)].the effect of bilirubin on biliary and renal excretion of methylglucamine-iodipamide at an infusion rate of 1.3, 2.6 and 5.2 mu mol/min/kg was studied by intravenous injection into five dogs with normal liver functions and chronic duodenal fistulas. unconjugated bilirubin administered in a dose of 0.1 mu mol/min/kg reduced biliary iodipamide excretion and concentration significantly, but did not affect renal contrast material excretion. this indicates that the unsatisficatory radiological result ...1979153309
[effects of combination alfaxalone and alfadolone, anesthetic derivatives of pregnanedione, on cerebral hemodynamics in cats].intravenous injection of ct 1341 (a mixture of alphaxalone and alphadolone dissolved in cremophor el) induced a decrease in cerebral blood flow (cbf) measured by 133xe clearance in cats with artificial respiration (the mean reduction in cbf was 2 ml/100 g/mn for 1,2 mg/kg or ct 1341. so, cbf was decreased by 22% when ct 1341 (7,2 mg/kg) was intravenously injected, (mean pa co2 equals 30 mm hg). changes in cbf following ct 1341 intravenous injection seems to be caused by cerebral vascular constri ...1975126719
persistence of ketosis despite suppression of lipolysis by 16,16-dimethylprostaglandin e2 in experimental diabetic ketoacidosis in rats. 1979113273
serial prolactin and thyrotropin responses to thyrotropin-releasing hormone throughout normal human pregnancy. 1979107178
ultrastructural localization of calcium around the membrane of the surface connected system in the human platelet.the localization of calcium in the membrane system of human platelets was determined by ultrahistochemical methods equipped with an electron probe x-ray microanalyzer. after potassium oxalate-glutaraldehyde treatment large amounts of electron opaque precipitates were observed around the membrane of the surface connected system. electron probe x-ray microanalysis clearly defined that the precipitates were composed of calcium oxalate. the localization of calcium on the membrane of the surface conn ...197896043
an electromyographic study of uterotubal activity in the ewe.the electrical activity of the oviduct and uterus was automatically registered on a potentiometric pen recorder in ewes fitted with an extracellular multielectrode assembly. the duration and amplitude of local non-propagating activity of the uterus increased and became progressively grouped into phases as natural oestrus progressed. phases of contractions lasting 5-6 min were initially propagated at a rate of 2/hr. their frequency increased within 24 hr of the decline in plasma progesterone leve ...197660491
proceedings of the fifteenth congress of the european dialysis and transplant association, istanbul, turkey, 1978. 197833380
tissue fractionation in neurobiochemistry: an analytical tool or a source of artifacts. 197722511
management of neurologic opportunistic disorders in human immunodeficiency virus infection. 19921615237
treatment with digestive agents reveals several glycoconjugates specifically associated with rat neuromuscular junction.the lectins dba, wga, sba, con a, gs-1, lfa and pna were used to characterize the carbohydrate domains of the rat neuromuscular junction. dba stained only the synaptic domains of muscle surface. all other lectins stained the whole muscle surface but the intensity of staining was stronger at the synaptic regions. however, when sections were treated with several digestive agents prior to lectin application, the lectin staining pattern changed dramatically. collagenase-sensitive glcnac, mannose, si ...19921559843
16s rrna phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects.bacterial endosymbionts of insects have long been implicated in the phenomenon of cytoplasmic incompatibility, in which certain crosses between symbiont-infected individuals lead to embryonic death or sex ratio distortion. the taxonomic position of these bacteria has, however, not been known with any certainty. similarly, the relatedness of the bacteria infecting various insect hosts has been unclear. the inability to grow these bacteria on defined cell-free medium has been the major factor unde ...19921557375
cyclic nucleotide-dependent regulation of agonist-induced calcium increases in mouse megakaryocytes.1. the regulatory effects of cyclic amp and cyclic gmp on adp- and thrombin-induced increases in [ca2+]i were studied in mouse bone marrow megakaryocytes. changes in [ca2+]i were continuously monitored in single fura-2-loaded cells using microspectrofluorometry, and cyclic nucleotides were directly introduced into the single cells using the whole-cell patch-clamp technique. 2. adp increased [ca2+]i in a concentration-dependent fashion, and its threshold concentration was in the order of 0.01 mic ...19921317440
comparative morphology of genital cones of genus ancylostoma dubini, 1843.male worms of ancylostoma braziliense, a. ceylanicum, a. kusimaense, a. malayanum, a. duodenale, a. caninum, a. tubaeforme, agriostomum vryburgi and cyclodontostomum purvisi have the external appendages beside the anogenital aperture. these anogenital structures are morphologically similar and are assumed to be homologous among the species with three pairs of teeth or more. in hookworms with two pairs of teeth, the anogenital structures are complex. anogenital features can be used as a taxonomic ...19751166351
changes in monoamine oxidase and monoamines with human development and aging.a series of studies of monoamines and their metabolism in a variety of human tissues indicate that there are aging effects that may alter neurotransmitter substances. monoamine oxidase (mao) activity has a significant positive correlation with age in plasma and blood platelets of normal subjects and patients suffering from depressive disorders. monoamine oxidase and age correlate positively in hindbrain and in eight separate ares of human brains from patients who died from a variety of causes. h ...19751088942
origin and early development of canine circumanal glands. 1979517821
development of an in vitro microtest to assess drug susceptibility of babesia bovis and babesia bigemina.continuous cultivation of the bovine hemoparasites babesia bovis and babesia bigemina was developed as an in vitro microtest to assess parasite susceptibility to babesicidal compounds. reproducibility of parasite multiplication rates was independent of culture size, making it possible to use a microscale of 100 microliters for each test sample. inhibitory concentrations (ic50s) of a commonly used babesicide, quinuronium sulfate, evaluated by this in vitro method were found to be 5 x 10(-8) g/ml ...19911779305
role of serine biosynthesis and its utilization in the alternative pathway from glucose to glycogen during the response to insulin in cultured foetal-rat hepatocytes.the role of serine as a possible intermediate of the alternative pathway from glucose to glycogen was investigated under basal and insulin-stimulated conditions in 18-day cultured foetal-rat hepatocytes because these cells cannot use pyruvate-derived metabolites [bismut & plas (1989) biochem. j. 263, 889-895]. incubation of cells with [u-14c]glucose for 24 h led to a release of labelled serine in the medium concomitantly with a net serine production (100 nmol/24 h per culture). the rate of [14c] ...19911905920
a primary demyelinating disorder of young cattle.the clinical signs and neuropathological changes are described in six limousin x calves that, at about one month after birth, showed signs of blindness, nystagmus, rotation of the eyes, opisthotonos, hyperprotraction of the forelegs and, in one case, apparent seizures. pathologically there was necrosis of the optic chiasma and focal areas of myelin sheath vacuolation or demyelination in certain areas of the brain, especially in the cerebellar peduncles. in one animal, kept alive for 7 months, th ...19911800911
rumpshaker mouse: a new x-linked mutation affecting myelination: evidence for a defect in plp expression.this report describes a new x-linked mutation in mice, named rumpshaker (rsh) which is associated with hypomyelination of the central nervous system. myelination commences appropriately but the majority of sheaths fail to develop normally. oligodendrocytes are increased in number and have prominent golgi apparatus, rough endoplasmic reticulum and free ribosomes. occasional cisternae of rough endoplasmic reticulum are distended. some dense lamellar inclusions occur in oligodendrocytes but overall ...19901694232
cloning and nucleotide sequence of a cdna encoding the antifungal-protein of aspergillus giganteus and preliminary characterization of the native gene. 19902374718
[the role of bile acids with physiological concentration in colon carcinogenesis].the promoting effect of bile acids in physiological concentration in colon carcinogenesis was studied using male wistar rats with defunctioned colon. after an instillation of mnng, collected rat faces (group a), secondary bile acids i.e. deoxycholic acid and lithocholic acid in equal concentration to rat feces (group b) and control material (group c) was instilled in the defunctioned colon. concerning the incidence and the number of macroscopically visible tumor, no significant difference was fo ...19892585788
molecular nature of a cell membrane antigen specific for human t-cell acute lymphoblastic leukemia.in the present work, we characterized the molecular nature of a t-cell acute lymphoblastic leukemia (t all) specific antigen, termed talla, which is defined by monoclonal antibody sn1. sn1 shows an extremely high specificity for t all. in the present study, sn1 was further shown not to react significantly with various normal solid tissues. talla was determined to be a glycoprotein with an approximate molecular weight of 150,000. however, the molecular nature of talla is peculiar in that heating ...19872440562
structural and biochemical changes in the sclera of experimentally myopic eyes. 19911794573
use of a most probable number method modified with a deoxyribonucleic probe to monitor control by food preservatives of natural salmonella contamination in animal meat meals.a most probable number (mpn) technique using a dna probe was developed for determination of salmonella in naturally contaminated meat meals. the method eliminated the need for 180 selective plates and 75 selective tubes and saved 27 h when compared with a traditional technique; yet it still yielded identical mpn of salmonella per 100 g in five naturally contaminated samples. the method was used to monitor the effect of a commercial food preservative (sal curb) at 0, .25, .50, 1.0, and 2.0% in me ...19911876558
immunohistochemical localization of phosphoenolpyruvate carboxykinase in adult and developing mouse tissues.we used immunohistochemical techniques to analyze the cell distribution of phosphoenolpyruvate carboxykinase (pepck) in adult and developing mouse tissues. pepck immunoreactivity was detected in many tissues, including some that had not been previously reported to contain pepck enzyme activity (bladder, stomach, ovary, vagina, parotid gland, submaxillary gland, and eye). in some multicellular tissues, pepck immunoreactivity was observed in multiple cell types. several tissues (spleen, thyroid, a ...19901688895
determinants of immunogenicity and mechanisms of protection by virulent and mutant vibrio cholerae o1 in rabbits.the colonizing capacities of 16 vibrio cholerae strains, including nine genetically and/or phenotypically defined parent-mutant pairs, were determined in unobstructed adult rabbit small bowel. there were marked interstrain differences in colonizing capacity, which was enhanced by bacterial motility and the production of cholera holotoxin but was unrelated to production of cholera toxin b-subunit or hemolysin or to bacterial serotype or biotype. the role of colonizing capacity and other bacterial ...19882447017
atypical leishmaniasis in an hiv-2-seropositive patient from guinea-bissau. 19911892600
modification of allergen-induced airway obstruction and airway hyperresponsiveness in an allergic rabbit model by the selective platelet-activating factor antagonist, bn 52021.allergen challenge of eight ragweed-immunized rabbits elicited a prolonged airway obstruction that was evident during the 6-hour study period. airway responsiveness to histamine was enhanced 24 hours after allergen exposure (geometric mean of the 50% reduction in effective dose [ed50] of dynamic compliance [cdyn] before challenge, 3.46 mg/ml, decreased to 0.52 mg/ml after challenge, p less than 0.01; pulmonary resistance ed50 geometric mean before challenge, 3.50 mg/ml, decreased to 0.65 mg/ml a ...19892600327
specificity of growth inhibition of melanoma by 4-hydroxyanisole.an experimental study using human melanoma (nel-mi), rat hepatoma (fu5-5), and human kidney (293-31) cell lines was undertaken in order to evaluate the antitumor activity of 4-hydroxyanisole (4-oha) in vitro. prior reports have indicated highly specific antitumor activity of 4-oha against melanoma cells in vitro. this specific antitumor activity has been proposed to be due to the oxidation of 4-oha by tyrosinase to cytotoxic oxidation products. dose-dependent cytotoxicity was observed when cells ...19892497444
effects of amino acid infusion on renal hemodynamics. role of endothelium-derived relaxing factor.ingestion of protein or intravenous infusion of amino acids acutely elevates glomerular filtration rate (gfr) and renal plasma flow (rpf) by unknown mechanisms. endothelium-derived relaxing factor (edrf), now known to be nitric oxide derived from metabolism of l-arginine, participates in local regulation of vascular tone. to investigate the hypothesis that edrf may participate in the renal vasodilatation and increased gfr after amino acid infusion, we characterized the effect of inhibition of ed ...19912045148
pharmacokinetics and tissue concentrations of cefuroxime.cefuroxime is well-absorbed and distributed over the human body. this review gives a general summary of published information on tissue concentrations of cefuroxime after an intravenous or intramuscular dose of 750 or 1,500 mg of cefuroxime. tissue concentrations are given for blister fluid, meninges, eyes, respiratory tract, sputum and bronchi, abdominal and urogenital tract, uterine, ovarian and fallopian tube tissue, myometrium, membranes, placenta, puruloid and healthy human milk, bile, pros ...19902075076
[experimental infection index of anopheles sinensis and melanization of periodic brugia malayi larvae].this paper deals with the infection index of anopheles sinensis infected with the blood meals of 9 different microfilarial densities (5-300 mf/10 microliters) of periodic brugia malayi. the results indicated that the mean number of microfilariae (mf) ingested by mosquitoes increased with the mf-density of the blood meal. the infection rates of vectors were 30, 65, 93 and 100%, when mf-densities were 5, 10, 20 and 50 mf/10 microliters respectively. although the infection rate was 100% when mf-den ...19902099254
in-vitro mutagenesis of the bovine growth hormone gene.the biological activities of bovine growth hormone (bgh) were studied in a transgenic mouse model system. the following experimental design was used: (1) in-vitro mutagenesis of the bgh gene; (2) expression of the mutated gene in cultured mouse cells under transcriptional regulation of the mouse metallothionein i promoter; (3) binding studies of the mutated and wild-type protein to mouse liver membrane preparations; (4) generation of transgenic mice which express the mutant hormone; and (5) grow ...19902213712
in-vitro mutagenesis of the bovine growth hormone gene.the biological activities of bovine growth hormone (bgh) were studied in a transgenic mouse model system. the following experimental design was used: (1) in-vitro mutagenesis of the bgh gene; (2) expression of the mutated gene in cultured mouse cells under transcriptional regulation of the mouse metallothionein i promoter; (3) binding studies of the mutated and wild-type protein to mouse liver membrane preparations; (4) generation of transgenic mice which express the mutant hormone; and (5) grow ...19902213712
a new assessment in vitro of human salivary lubrication using a compliant substrate.the lubrication effect of salivary secretions was assessed in terms of separating a rigid object from a compliant substrate. there was little difference among the various secretions of a single donor. the viscosity of salivas increased as a function of time. neither the friction testing nor viscometry provided an adequate model of the tissue-coating function ascribed to saliva.19902222251
decreased cyclic amp degradation in ng 108-15 neuroblastoma x glioma hybrid cells and s49 lymphoma cells chronically treated with drugs that inhibit adenylate cyclase.the increase in hormone-stimulated cyclic amp accumulation observed in a variety of intact cells after chronic pretreatment with drugs that inhibit adenylate cyclase activity has been attributed to an increase in adenylate cyclase activity following withdrawal of the inhibitory drug. in ng 108-15 mouse neuroblastoma x rat glioma hybrid cells (ng cells) chronically treated with the muscarinic cholinergic agonist carbachol, we have found a significant decrease in the apparent degradation rate cons ...19901688917
[effect of enalapril on heart rate, arterial blood pressure and exocrine pancreatic secretion in the alert dog].a changed exocrine pancreatic secretion could be a pathogenetic factor of an acute pancreatitis after administration of angiotensin-converting enzyme (ace) inhibitors. in six conscious dogs with gastric and duodenal thomas fistulas we studied the effect of an intravenous (iv.) bolus injection of 10 mg enalaprilat, an intraduodenal (id.) bolus injection of 20 and 40 mg enalapril (e.), and 0.15 m nacl (20 ml iv., resp., id.) on pancreatic bicarbonate- and protein output in response to secretin (20 ...19902238764
human gastric mucosa expresses glandular m3 subtype of muscarinic receptors.five subtypes of muscarinic receptors have been distinguished by pharmacological and molecular biological methods. this report characterizes the muscarinic subtype present in human gastric mucosa by radioligand binding studies. the receptor density was 27 +/- 6 fmol/mg protein and the tritiated ligand n-methylscopolamine had an affinity of (kd) 0.39 +/- 0.08 nm (n = 11). the m1 receptor selective antagonist pirenzepine and the m2 receptor selective ligand af-dx 116 had low affinities of 148 +/- ...19902253531
ca2+ transients are not required as signals for long-term neurite outgrowth from cultured sympathetic neurons.a method for clamping cytosolic free ca2+ ([ca2+]i) in cultures of rat sympathetic neurons at or below resting levels for several days was devised to determine whether ca2+ signals are required for neurite outgrowth from neurons that depend on nerve growth factor (ngf) for their growth and survival. to control [ca2+]i, normal ca2+ influx was eliminated by titration of extracellular ca2+ with egta and reinstated through voltage-sensitive ca2+ channels. the rate of neurite outgrowth and the number ...19902324199
biochemical mechanisms of aminoglycoside cell toxicity. ii. accumulation of phospholipids during myeloid body formation and histological studies on myeloid bodies using twelve aminoglycoside antibiotics.cultured human skin fibroblasts take up aminoglycoside antibiotics into lysosomes to form myeloid bodies. gentamicin (gm), one such antibiotic, was taken up until the cellular concentration reached an estimated 64 mm on the 3rd day when cells were incubated with 2 mm gentamicin. the rate of release of intracellular gm was high on the first day of incubation and gradually slowed down over the next 4 d. about 50% of the gm remained in the cells even on longer incubation in gm-free medium, suggesti ...19892613686
repair of experimental arteriotomy in rabbit aorta using a new resorbable elastin-fibrin biomaterial.a new artificial connective matrix which results from two reactions of fibrinogen and fibronectin on elastin was used to obturate a slit made in the abdominal aorta of rabbit. the so-called elastin-fibrin biomaterial behaved as a scaffold through which all the different structures were restored to their former condition. at 3 months, the material had disappeared and no thrombus, no inflammation or reject had been detected.19892621215
pancreas-graft immunogenicity and pretreatment with anti-class ii monoclonal antibodies.pretreatment of organ allografts to reduce graft immunogenicity is an attractive and potentially clinically applicable concept. we have studied the effect of perfusing rat pancreases with anti-class ii monoclonal antibody (moab), to remove class ii- positive accessory cells from the intact organ, on prolongation of allograft survival after transplantation. the capacity of pancreatic islets obtained from these perfused organs to stimulate proliferation of allogeneic t-lymphocytes was studied in a ...19892642828
heterogeneity of the streptokinase gene in group a streptococci.a molecular epidemiological study was conducted to determine the distribution of the streptokinase gene in group a streptococcal strains of different m types and in other streptococcal species. plasmid pnc1, containing only the internal coding sequence of the streptokinase gene from group c streptococcal strain h46a, was used as a dna probe in colony and southern hybridization experiments. only the pathogenic group a, c, and g streptococci contained a streptokinase gene; 12 other lancefield grou ...19892643572
secretagogue-induced changes in intracellular ph and amylase release in mouse pancreatic acini.the response of the intracellular ph (phi) to stimulation of enzyme secretion in pancreatic acini was measured using the fluorescent dye 2'-7'-bis(carboxyethyl)-5(6)-carboxyfluorescein. acini suspended in ph 7.40 buffer demonstrated cytoplasmic alkalinization of 0.17, 0.14, and 0.15 ph units 2 min after addition of the secretagogues carbachol (10(-5) m), caerulein (10(-10) m), and bromo-a23187 (10(-6) m). corresponding net stimulated amylase secretion over 30 min was 9.2, 10.3, and 5.6% of total ...19872446509
heterogeneity of the streptokinase gene in group a streptococci.a molecular epidemiological study was conducted to determine the distribution of the streptokinase gene in group a streptococcal strains of different m types and in other streptococcal species. plasmid pnc1, containing only the internal coding sequence of the streptokinase gene from group c streptococcal strain h46a, was used as a dna probe in colony and southern hybridization experiments. only the pathogenic group a, c, and g streptococci contained a streptokinase gene; 12 other lancefield grou ...19892643572
autosomal dominant retinitis pigmentosa: exclusion of a gene from extensive regions of chromosomes 6, 13, 20, and 21.members of a large pedigree of irish origin presenting with early onset type i autosomal dominant retinitis pigmentosa (adrp) have been typed for polymorphic dna markers from chromosomes 6, 13, 20, and 21. for each marker close linkage to adrp has been excluded by pairwise analyses. using distances fixed from well-established genetic maps of these chromosomes and multipoint analyses with two or three contiguous markers, exclusion of adrp was extended to the areas between markers, resulting in th ...19892613243
[alimentary outbreak of tonsillitis]. 19892657209
monoclonal anti-il2-receptor in organ transplantation.so far, only monoclonal antibodies directed at functional target molecules on lymphocyte surface membrane have been proven useful in preventing or reversing allograft rejection episodes. antibodies directed against light chain (p55) of interleukin 2 (il2) receptor and able to interfere with il2 binding on the il2-receptor in its high-affinity conformation (only expressed on activated t cells) are effective in various animal models and recently in a preventive protocol in human kidney graft recip ...19892669805
speech development in children.speech is unique to man, as a system of communication in which thoughts are expressed and understood by using acoustic symbols. the range of normality in speech development in children is wide. development does not occur at a steady rate and language milestones are earlier in girls. there is speech delay if the child is not speaking by 3 1/2 years of age, or speech may be defective in articulation or language. any speech problems should be detected and treated however as early as possible and he ...19892671496
molecular genetics of galactose metabolism in yeast. 19892679921
studies on 2,3,n,n'-substituted 4,4'-diaminodiphenylsulfones as potential antimalarial agents.a series of new 4,4'-diaminodiphenylsulfones substituted at 2 and 3 position and also at primary amino group of the phenyl rings have been synthesized and evaluated for their antimalarial activity against plasmodium berghei infection in mice. some of these compounds were active and showed complete inhibition of parasitaemia which included 7a1-7a4, 7b3, 7b4 and 16a at 1 mg/kg i.p. for 4 days and 16a, at 0.3 mg/kg for 4 days. some compounds tested for their synthetase inhibitory action in cell-fre ...19892686657
effect of sodium artesunate on malaria infected human erythrocytes.oxidative stress in malaria infected human erythrocytes is augmented and the anti-oxidant system is attenuated as compared with normal rbc's. exacerbation of intra-erythrocytic oxidative stress might provide a means to kill the parasites. sodium artesunate (sa), an effective chinese anti-malaria drug, markedly increased the levels of active oxygen species and production of malonyldialdehyde in normal red blood cells and, to a greater extent, in malaria infected red blood cells. sa caused a remar ...19892698476
inhibition of rat mesangial cell mitogenesis by nitric oxide-generating vasodilators.recent studies indicate that endothelium-derived relaxing factor (edrf) may be identical with nitric oxide (no). the purpose of this study was to investigate the antimitogenic effect of no-generating drugs in cultured mesangial cells. s-nitroso-n-acetylpenicillamine, sodium nitroprusside, and isosorbide dinitrate, which generate no, dose dependently inhibited serum-stimulated dna synthesis. all three drugs also inhibited the rate of cell proliferation, whereas sodium nitroprusside and s-nitroso- ...19892546445
mutational specificity and the influence of excision repair: insights into the mechanisms of error-avoidance and error-fixation.the excision repair process controlled by the uvrabc gene in escherichia coli is the major pathway for the repair of a diverse series of dna damages. to achieve a better understanding of the mechanics of this repair pathway and its impact upon mutagenesis, we have applied a recently developed technology by which the nature of mutation is determined at the dna sequence level. a comparison of the classes and distribution of mutation in excision-repair-proficient and excision-repair-deficient strai ...19892698833
studies of the phosphorylation of escherichia coli isocitrate dehydrogenase. recognition of the enzyme by isocitrate dehydrogenase kinase/phosphatase and effects of phosphorylation on its structure and properties.escherichia coli isocitrate dehydrogenase is completely inactivated by phosphorylation of a single serine residue per subunit. we have examined the conformations of the active and phosphorylated forms of the enzyme using circular dichroism spectroscopy. the results support the view that phosphorylation prevents the binding of nadp, probably by direct blocking of the coenzyme-binding site. labelling studies suggest that an arginine residue at the coenzyme-binding site may be close to the phosphor ...19892557094
antagonism of central and peripheral anorectic effects of caerulein by l-364,718.the reduction in food intake induced by i.p. injections of the cholecystokinin (cck) analogue caerulein was antagonised by a low dose (0.25 mumol/kg s.c.) of the selective cck antagonist l-364,718. to block the anorectic effect of centrally administered caerulein a dose of 25 mumol/kg of l-364,718 was required, demonstrating that central cck receptors can be blocked effectively in the rat by choosing appropriate doses of l-364,718.19892721558
antagonism of central and peripheral anorectic effects of caerulein by l-364,718.the reduction in food intake induced by i.p. injections of the cholecystokinin (cck) analogue caerulein was antagonised by a low dose (0.25 mumol/kg s.c.) of the selective cck antagonist l-364,718. to block the anorectic effect of centrally administered caerulein a dose of 25 mumol/kg of l-364,718 was required, demonstrating that central cck receptors can be blocked effectively in the rat by choosing appropriate doses of l-364,718.19892721558
in vivo biology of recombinant interleukin-2 infusion in sheep: cardiopulmonary manifestations of an intravascular immune-inflammatory response.we have described the physiologic changes that occur in the conscious sheep during five days of dosing with ril-2. major effects noted were a decrease in systemic vascular resistance (vasodilation) accompanied by an increase in cardiac output (blood flow) and heart rate and a mild increase in pulmonary vascular resistance (vasoconstriction). body temperature increased and may have contributed to the peripheral vasodilation. lung microvascular fluid and protein flux increased following ril-2 admi ...19892787783
thio-analogues of inorganic pyrophosphate inhibit the replication of influenza virus a in vitro.mono- and bisthiopyrophosphate can inhibit the replication of influenza virus a/x49 in madin-darby canine kidney (mdck) cells at concentrations at which no cytotoxic effect is observed after 3 days. the thiopyrophosphate analogues inhibit the rna transcriptase activity of this virus possibly by chelating with an essential metal ion in the transcriptase complex. [31p]nmr spectroscopy indicates that bisthiopyrophosphate coordinates to zinc through sulphur and magnesium through oxygen which may inf ...19852990333
morphometric analysis of the cell outlines of normal and polyomavirus-transformed fr 3t3 fibroblasts.a method is described for the analysis of cell shape, using an image analyzer connected to a computer to assess the cell outline. a series of parameters to assess the contribution of large cytoplasmic expansions to cell morphology and to cell spreading on a planar substratum were used to quantify the visual morphologic differences between normal (nontransformed; n.3t3) and polyomavirus-transformed (py.3t3) fisher rat 3t3 fibroblasts. the results show that the py.3t3 fibroblasts are more spherica ...19872822058
reinfection of guinea pigs with herpes simplex virus type 2: failure to establish a second latent ganglionic infection.guinea pigs were infected with herpes simplex virus type 2 (hsv 2) and at various times thereafter reinfected at a different site with the same or a different strain of hsv 2. viruses were isolated from infected tissues either from the site of primary or secondary infection or from regional ganglia. viral isolates were characterised by dna fingerprinting using restriction endonucleases capable of differentiating between the virus strains used. the second infection resulted in a second site of pe ...19872825951
[suppurative meningitis in the newborn infant: experience with 107 cases in the ivory coast].a retrospective study of 107 neonates with meningitis showed that in 45% of cases the condition occurred during the first 48 hours after birth, probably following a materno-foetal infection. male neonates accounted for 70% of the cases. in 15% of cases, the mothers previously had a known infectious disease and 55% of cases came from an unfavourable socio-economic environment. over 50% of the infants had to be resuscitated at birth. the majority of organisms isolated were gram-negative bacteria o ...19882834748
two mutant alleles of the insulin receptor gene in a patient with extreme insulin resistance.insulin receptor complementary dna has been cloned from an insulin-resistant patient with leprechaunism whose receptors exhibited multiple abnormalities in insulin binding. the patient is a compound heterozygote, having inherited two different mutant alleles of the insulin receptor gene. one allele contains a missense mutation encoding the substitution of glutamic acid for lysine at position 460 in the alpha subunit of the receptor. the second allele has a nonsense mutation causing premature cha ...19882834824
two mutant alleles of the insulin receptor gene in a patient with extreme insulin resistance.insulin receptor complementary dna has been cloned from an insulin-resistant patient with leprechaunism whose receptors exhibited multiple abnormalities in insulin binding. the patient is a compound heterozygote, having inherited two different mutant alleles of the insulin receptor gene. one allele contains a missense mutation encoding the substitution of glutamic acid for lysine at position 460 in the alpha subunit of the receptor. the second allele has a nonsense mutation causing premature cha ...19882834824
ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase in rat and bovine brain tissues.inositol 1,4,5-trisphosphate (ins p3) 3-kinase catalyzes the atp-dependent phosphorylation of ins p3 to inositol 1,3,4,5-tetrakisphosphate (ins p4). ca2+/calmodulin (cam)-sensitivity of ins p3 3-kinase was measured in the crude soluble fraction from rat brain and different anatomic regions of bovine brain. kinase activity was inhibited in the presence of egta (free ca2+ below 1 nm) as compared to ca2+ (10 microm free ca2+) or ca2+ (10 microm free ca2+) and cam (1 microm). ca2+-sensitivity was al ...19882838022
legionella in power station cooling waters. 19852862486
multilayer-enzyme linked immunosorbent assay (ml-elisa) for detection of entamoeba histolytica trophozoite coproantigen.multilayer elisa (ml-elisa) method for entamoeba histolytica trophozoite coproantigen was developed for diagnosis of amoebiasis and its usefulness vis-a-vis microscopic examination analysed for paired specimens from 87 patients from lucknow and hyderabad, india. all the stool specimens positive for trophozoites were also found to be positive in ml-elisa. out of a total 32 samples positive for cysts in microscopy 21 (65.62%) gave positive reactions for coproantigen. results on 26 samples drawn fr ...19852867033
phosphoenolpyruvate carboxykinase as a selective indicator of ochratoxin a induced nephropathy. 19862881768
the leeuwenhoek lecture, 1986. environmental carcinogens and papillomaviruses in the pathogenesis of cancer.in many areas of the world there is a geographically localized high incidence of alimentary and bladder cancer in cattle. studies in western scotland have demonstrated that this phenomenon is associated with ingestion of bracken fern. however, the affected animals and herds were shown also to have an unusually high infection rate of alimentary papillomas caused by a previously unrecognised bovine papillomavirus (bpv) and that these tumors could undergo malignant transformation. long-term field a ...19872888116
hincii and kpni rflps for laminin b1 (lamb1) gene on chromosome 7. 19882901729
chronic cardiac toxicity after inhalation of 1,1,1-trichloroethane.two patients showed evidence of chronic cardiac toxicity after repeated exposure to 1,1,1-trichloroethane. in both cases there was circumstantial evidence of a deterioration after routine anaesthetic use of the related compound halothane. an adolescent boy who sniffed trichloroethane presented with multiple ventricular arrhythmias during tonsillectomy. follow up showed mild chronic left ventricular impairment. a 54 year old man had repeated industrial exposure to trichloroethane and deteriorated ...19873105712
administration of gm1 ganglioside eliminates neuroleptic-induced sensorimotor deficits in mptp-treated mice.injection of a low dose of haloperidol, that has no obvious behavioral effects in normal mice, produces akinesia, catalepsy, and sensory neglect in mptp-treated mice. gm1 ganglioside treatment eliminates all of these behavioral impairments and also partially restores striatal dopamine content. these observations suggest that the mptp-treated mouse may be a valuable model for studying mechanisms underlying parkinsonism and that administration of gm1 ganglioside may be an effective therapy.19882903475
dose-related effects of synthetic human beta-endorphin and naloxone on fed gastrointestinal motility.in humans, plasma beta-endorphin levels rise during application of acute stressful stimuli (vertigo, cold pain, and transcutaneous electrical stimulation) that induce gut motor disturbances. whereas it is possible that circulating beta-endorphin participates in the mediation of these central effects on gut motility, its role cannot be established solely on the basis of changes in plasma levels. therefore, we designed the present study to investigate 1) the dose-related effects of intravenous syn ...19862942039
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...19862946570
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...19862946570
reca protein-promoted atp hydrolysis occurs throughout reca nucleoprotein filaments.when reca protein binds cooperatively to single-stranded dna to form filamentous nucleoprotein complexes, it becomes competent to hydrolyze atp. no correlation exists between the ends of such complexes and the rate of atp hydrolysis. atp hydrolysis is not, therefore, restricted to the terminal subunits on cooperatively bound reca oligomers, but occurs throughout the complex. similarly, during reca protein-promoted branch migration (during dna strand exchange), atp hydrolysis is not restricted to ...19872951381
identification of apolipoproteins involved in the interaction of human high density lipoprotein3 with receptors on cultured cells.human high density lipoprotein (hdl), devoid of apolipoproteins e or b, binds with high affinity and specificity to cultured cells derived from several tissues. in order to investigate the ligand specificity of the putative receptor, we have performed competitive inhibition studies to identify the components of high density lipoprotein that bind to cell surfaces of rat adrenal cortical cells and human skin fibroblasts. radiolabeled hdl3 was displaced with unlabeled apolipoprotein-dimyristoylphos ...19852982866
use of liposomes for protective immunisation against crotalus durissus (tropical rattlesnake) venom.crotalus durissus venom has been described as a weak antigen when injected in combination with freund's complete adjuvant during the course of traditional methods of equine immunisation. antibody production is slow and unpredictable, with a wide variation in individual responses. in this experimental study, c. durissus venom was incorporated into stabilised sphingomyelin-cholesterol liposomes both in the presence and absence of lipopolysaccharide immunostimulant and injected by both i.v. and s.c ...19892728026
presynaptic inhibition of the monosynaptic reflex during local tetanus in the cat.tetanus toxin at doses of 2-2000 mouse mld/kg was injected into the gastrocnemius muscle of the left hind limb of the cat. acute experiments were performed at various times thereafter, when the intoxicated hind leg was strongly extended. presynaptic inhibition of the monosynaptic reflex (msr) of gastrocnemius motoneurones was tested by applying conditioning single electric stimuli to the antagonistic deep peroneal nerve. in most intoxicated animals the delayed inhibition of the msr could still b ...19892728030
passive protection of calves against experimental infection with salmonella typhimurium.cows were vaccinated with formalin-killed salmonella typhimurium approximately seven weeks and two weeks before parturition to investigate whether passive immunity could protect their calves against experimental s typhimurium infection. after birth the calves were left with their dam for 48 hours and then separated and fed cold, stored colostrum from their own dam for a further eight days. oral challenge five days after birth with 10(8) s typhimurium did not result in the death of these calves e ...19883061151
passive protection of calves against experimental infection with salmonella typhimurium.cows were vaccinated with formalin-killed salmonella typhimurium approximately seven weeks and two weeks before parturition to investigate whether passive immunity could protect their calves against experimental s typhimurium infection. after birth the calves were left with their dam for 48 hours and then separated and fed cold, stored colostrum from their own dam for a further eight days. oral challenge five days after birth with 10(8) s typhimurium did not result in the death of these calves e ...19883061151
presynaptic opioid kappa-receptor and regulation of the release of met-enkephalin in the rat brainstem.opioid kappa-agonists had much more potent inhibitory effects on the high k+-evoked met-enkephalin release from rat brain slices than did the mu- or delta-agonists. the opioid kappa- antagonist, mr2266 enhanced the evoked release of met-enkephalin to a greater extent than did mu- or delta-antagonists in vitro and had a potent analgesia in mice in vivo. these findings suggest that the release of met-enkephalin may be regulated in vitro and in vivo, mainly by presynaptic kappa-receptor-mediated me ...19872829060
[osmotic resistance of erythrocytes in cattle]. 19863087113
cellular distribution of lysosomal hydrolase activities in the regenerating rat liver.cathepsins b and d, beta-galactosidase, and acid phosphatase activities were found to be decreased in the regenerating rat liver, the reduction being maximal around the peak of hepatocyte mitoses (30 h). to investigate whether these changes could be heterogeneously distributed among hepatic cells, total cell populations from control or two-thirds hepatectomized rat livers were dissociated by the collagenase perfusion technique and analysed by different procedures. isopycnic centrifugation in a m ...19863089641
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