| binding studies with antibodies having phosphorylcholine specificity and fragments derived from their homologous streptococcus pneumoniae type 27 capsular polysaccharide. | the capsular polysaccharide from streptococcus pneumoniae type 27 (s-27) has been fragmented into oligosaccharides by controlled degradations. binding of these derived small ligands with heterogeneous rabbit anti-s-27 antibody of selected phosphorylcholine affinity indicates that a major immunodeterminant of s-27 is the phosphorylcholine moiety, but that these anti-pc. antibodies are also capable of binding to the polysaccharide backbone from which the p.c. has been removed. | 1979 | 87460 |
| guinea pig transfer factor: restriction of macrophage migration inhibitory activity to extraordinary dilution confirmed with demonstration of diminished potency of stored preparations. | | 1979 | 88292 |
| characterization of functional fc-receptor material from human lymphoblastoid cell lines. ii. serologic and cellular analysis. | | 1979 | 88475 |
| prolonged survival of corneal allografts in rabbits treated with cyclosporin a. | | 1979 | 90771 |
| effect on rats and mice of a diet with low tryptophan content (maize flour). | female wistar rats when crowded together become aggressive if fed a diet with low tryptophan (trp) content (maize, flour, mf). when isolated during 30 days and fed mf they show a decrease of weight, of total plasma trp, and of trp and 5-hydroxytryptamine (5-ht) in brain stem, but 5-hydroxyindolacetic acid (5-hiaa) of the same cerebral area does not change. these results partially agree with other authors observations. mice isolated during 47 days and fed with mf do not show any significant weigh ... | 1979 | 95611 |
| ultrastructural localization of calcium around the membrane of the surface connected system in the human platelet. | the localization of calcium in the membrane system of human platelets was determined by ultrahistochemical methods equipped with an electron probe x-ray microanalyzer. after potassium oxalate-glutaraldehyde treatment large amounts of electron opaque precipitates were observed around the membrane of the surface connected system. electron probe x-ray microanalysis clearly defined that the precipitates were composed of calcium oxalate. the localization of calcium on the membrane of the surface conn ... | 1978 | 96043 |
| [immuno-electro-diffusion on cellulose acetate membrane for serological testing in human malaria (author's transl)]. | an immuno-electro-diffusion assay for human malaria, using cellulose acetate gel, is described. more than 3500 sera have been tested by this method, which proved very useful for epidemiology since 400 sera a day can be tested by one person, with simple equipment. its sensitivity compared well to the ifa test, although the antibodies revealed are quite different. a constnat precipitin line appeared, against 56 degrees c labile antigens, in sera of subjects infected by p. falciparum, and up to 8 o ... | 1978 | 98748 |
| [immunological study on rickettsia sennetsu by complement-fixation test (author's transl)]. | | 1978 | 101610 |
| [studies on the causative agent of hyuga-feveri; cultivation of rickettsia-like organisms isolated from metacercariae of stellantchasumus falcatus in tissue culture cell and their antigenic relation to rickettsia sennetsu (author's transl)]. | | 1978 | 102710 |
| guinea pig immunoglobulin light chain isotypes. i. separation of kappa and lambda chains and the identification of three isotypes of the lambda chain constant homology region. | the separation of intact kappa chain and two fragments comprising lambda (lambda) chain from immunoglobulin light chain pools isolated from strain 13 guinea pigs is achieved by cyanogen bromide digestion and gel filtration before and after reductive clevage of disulfide bonds. the smaller lambda chain fragment derives from the original carboxyl-terminus of lambda chain. the partial sequence of component tryptic and thermolytic peptides of this thirty-nine residue fragment allowed its complete se ... | 1978 | 103729 |
| effects of chronic epileptic foci on control of pyramidal tract neurons in monkeys. | five macaca mulatta monkeys were operantly conditioned to control the firing patterns of single precentral pyramidal tract neurons. the accuracy with which the monkeys could control normal ptns from within the focus was significantly poorer than ptns from contralateral, homotopic cortex. in comparison to nonepileptic monkeys, there was no significant difference in the accuracy with which ptns from cortex contralateral to interictal foci were controlled. by contrast, comparison of the time necess ... | 1978 | 104868 |
| interference of iodine-125 ligands in radioimmunoassay: evidence implicating thyroxine-binding globulin. | although there is abundant published evidence that radioiodinated antigens interfere in digoxin radioimmunoassays, other radioimmunoassays are similarly affected. we investigated the relationship of radioiodinated antigen structure to its function in the immunoassay. carrier-free 125i-labeled iodotyrosine and iodohistamine derivatives were incubated with human serum, and the bound and free fractions were separated. we demonstrated that diiodotyrosyl analogs bind avidly to serum proteins. because ... | 1979 | 108034 |
| rapid serotyping of groups a, b, and c meningococci by rocket-line immunoelectrophoresis and co-agglutination. | rocket-line immunoelectrophoresis (r-lie) with antigen containing intermediate gel, and co-agglutination utilising protein a-containing staphylococci coated with specific antibodies, were adapted for serotyping the prototypes of group b meningococci. both were found to have the same specificity as agar gel double diffusion (agdd) but they were more sensitive and more rapid than agdd. r-lie required, like agdd, the extraction of relatively large quantities of bacteria, while the co-agglutination ... | 1979 | 108320 |
| [study on sennetsu rickettsiosis: seroepidemiological survey of rickettsia sennetsu in miyazaki district (author's transl)]. | | 1979 | 119016 |
| [immunological studies of rickettsial infection--analysis of lymphoid cell subpopulations of the spleens of the mice infected with rickettsia sennetsu and rickettsia tsutsugamushi (author's transl)]. | | 1979 | 119018 |
| [multiplication and distribution of rickettsia sennetsu in experimentally infected mouse spleen (author's transl)]. | | 1979 | 120399 |
| [immunocytological study of the gonadotrophic and thyrotrophic cells of the rat adenohypophysis]. | on paraffin or semi-thin sections various anti-lh or anti-tsh sera stain indifferently all the thyrotrophs and the gonadotrophs. inversely anti-beta-tsh, anti-beta-lh or anti-beta-fsh purified sera permit the discrimination of these two cell populations. the constancy of fixation of the anti-beta-lh and anti-beta-fsh sera on all the gonadotrophs gives evidence of their ability to produce both fsh and lh. however in a few female rats the central gonadotrophs are stained more weakly by anti-beta-f ... | 1977 | 145301 |
| [intravenous cholangiography in hyperbilirubinaemia (author's transl)]. | the effect of bilirubin on biliary and renal excretion of methylglucamine-iodipamide at an infusion rate of 1.3, 2.6 and 5.2 mu mol/min/kg was studied by intravenous injection into five dogs with normal liver functions and chronic duodenal fistulas. unconjugated bilirubin administered in a dose of 0.1 mu mol/min/kg reduced biliary iodipamide excretion and concentration significantly, but did not affect renal contrast material excretion. this indicates that the unsatisficatory radiological result ... | 1979 | 153309 |
| diversity of androgen effects in adulthood : a search for possible causes outside the effector apparatus of the target cells. | | 1975 | 171879 |
| sex specific oestrogen binding in the middle hypothalamus of rats. | | 1975 | 179802 |
| malignant transformation-linked imbalance: decreased xanthine oxidase activity in hepatomas. | xanthine oxidase was decreased 2- to 10-fold in all examined rat hepatomas irrespective of the malignancy; growth rate and degrees of histological differentiation of the neoplasms. the affinity to substrate (km=6-8 mum) and the ph optimum (8.0) of the liver and hepatoma enzymes were the same. the reprogramming of gene expression, as manifested in the decreased activity of this key purine metabolizing enzyme, appears to be specific to neoplastic transformation. since glutamine prpp amidotransfera ... | 1975 | 179860 |
| isolation and localization of rna fractions able to transfer tumor-specific delayed hypersensitivity in vitro. | rna fractions were prepared by sucrose density gradient centrifugation from hot-cold phenol, rna-rich extracts of lymphoid tissues from strain 2 guinea pigs hyperimmunized to line 1 or line 10 tumors. each rna fraction was assessed for its ability to convert nonsensitized strain 2 peritoneal exudate cells to a state of specific sensitivity for line 1- or line 10-solubilized tumor antigens. an rna fraction residing between the 4 s and 18 s peaks, designated as fraction "b", transferred line 10 or ... | 1976 | 187327 |
| the presence of disulfide-linked gp70-p15(e) complexes in akr murine leukemia virus. | | 1977 | 201090 |
| cytotoxic activities of normal cultured human t cells. | normal human t cells grown in continued cultures in medium containing conditioned medium (cm) from pha-stimulated lymphocytes were studied for their ability to manifest three known forms of cell-mediated cytotoxicity: lectin-induced cellular cytotoxicity (licc), natural killer cell (nk) activity, and antibody-dependent cellular cytotoxicity (adcc). the cultured t cells (ctc) were very effective mediators of licc, being cytotoxic even at very low attacker-target cell ratios in the presence of dif ... | 1978 | 308961 |
| [determination of the localization of the kcpa gene on the chromosome of e. coli o124. i. use of the type f' plasmids of varying length]. | the loss of virulence was observed in some of the transconjugates of e. coli o124 in the process of the transfer of the plasmids differing in length: f' 200 ps, f'x 573 and f'x 363, carrying the genes of the chromosome area lac--pur e. the genetic determinants contributing to the ability of e. coli o124 to produce keratoconjunctivitis seem to be localized near the lactose marker, and not near e as in sh. flexneri. in e. coli o124 these genetic determinants are probably localized on both sides of ... | 1979 | 373340 |
| effect of age, weight and adequacy of zinc intake on the balance between alkaline ribonuclease and ribonuclease inhibitor in various tissues of the rat. | 1. deficiency of zinc inhibits growth and also increases the activity of alkaline ribonuclease in certain tissues of the rat (prasad & oberleas, 1973). zn could influence ribonuclease activity by direct effects on the enzyme or its natural inhibitor, or non-specifically as occurs when growth rate is affected by various other factors. these possibilities were studied. 2. alkaline ribonuclease was shown to be inhibited by zn in vitro, but the concentrations of zn required were so high that the enz ... | 1978 | 415757 |
| effects of ccnu on hematopoiesis in rats. | the effect of orally administered ccnu on proliferating and cytokinetically "resting" bone marrow cells was studied in rats. the resting bone marrow cells were selectively labeled with 3h-thymidine by the "complete 3h-thymidine labeling method." the results show a reduction in the number of absolute bone marrow cells to about 40% of the original value in 3 days. regeneration was completed only after 22 days. no difference was found in the destructive or the regenerative pattern of cfu-c, myelopo ... | 1979 | 427820 |
| comparison of individual and cumulative dose-response curves [proceedings]. | | 1979 | 497601 |
| effect of cyclophosphamide on the growth of rickettsia sennetsu in experimentally infected mice. | the growth rate of rickettsia sennetsu, the etiological agent of sennetsu rickettsiosis, a special type of infectious mononucleosis found in western japan, is very low when the ordinary experimental methods used for other members of the rickettsiae are employed. attempts have been made to increase its growth the rickettsiae are employed. attempts have been made to increase its growth rate in cells and animals. by treating mice with cyclophosphamide, considerable enhancement of growth of the rick ... | 1975 | 1165122 |
| purification of complement-fixing antigens of rickettsia sennetsu by ether treatment. | soluble and particulate complement-fixing antigens of rickettsia sennetsu were prepared from spleen suspensions of mice infected with the rickettsia and treated with cyclophosphamide. the medium used during purification of antigens, a solution consisting of equal volumes of phosphate-glutamate-sucrose buffer and veronal-buffered saline, was suitable for obtaining antigens with high titers and no anti-complementary activity. by heat treatment, it was demonstrated that the soluble antigen was heat ... | 1976 | 1278997 |
| antigenic characterization of ehrlichiae: protein immunoblotting of ehrlichia canis, ehrlichia sennetsu, and ehrlichia risticii. | in recent years a febrile illness apparently associated with tick bite in patients in the united states has been attributed to infection by an ehrlichia species. this implication is based on serologic responses to e. canis, morphologic demonstration of ehrlichiae in clinical materials, and a single isolate distinct from e. canis which was obtained from a human patient by the centers for disease control. little is known about the antigens of the ehrlichiae. this report expands the breadth of avai ... | 1992 | 1583101 |
| time course of cerebral blood flow and histological outcome after focal cerebral ischemia in rats. | the relation between time-dependent changes in cerebral blood flow and the appearance of infarction after focal cerebral ischemia is still a matter for debate. the aim of this study was to measure perfusion after simultaneous occlusions of the left middle cerebral artery and ipsilateral common carotid artery in rats and correlate it with the timing and distribution of histological changes. | 1992 | 1636189 |
| antigenic and genomic relatedness among ehrlichia risticii, ehrlichia sennetsu, and ehrlichia canis. | antigenic and genomic relatedness among ehrlichia risticii, e. sennetsu, and e. canis was analyzed by enzyme-linked immunosorbent assay, western blotting (immunoblotting) and dna-dna hybridization. e. risticii and e. sennetsu were serologically related, and their western blot antigen profiles were nearly identical. two antigens of e. sennetsu corresponding to the 28- and 51-kda antigens of e. risticii were apparently larger than the e. risticii antigens, and the 55-kda antigen of e. risticii app ... | 1992 | 1736961 |
| the tribe ehrlichieae and ehrlichial diseases. | the tribe ehrlichieae consists of gram-negative minute cocci that are obligate intracellular parasites classified in the family rickettsiaceae. although ehrlichial organisms have been observed in leukocytes for many years, only a few species have been cultured in quantities sufficient for biochemical and molecular analyses. recents studies on 16s-rrna sequence analysis and energy metabolism showed that the genus ehrlichia is closely related to the genus rickettsia. there is, however, no antigeni ... | 1991 | 1889044 |
| in vitro susceptibility of ehrlichia sennetsu to antibiotics. | antibiotic efficacies were evaluated by diff-quik (dade, düdingen, federal republic of germany) staining of ehrlichia sennetsu in p388d1 murine macrophages grown in 96-well microtiter plates. sennetsu disease is generally cured with tetracyclines. in vivo, e. sennetsu is susceptible to doxycycline and is resistant to erythromycin, penicillin, and chloramphenicol. our study confirmed, in vitro, the efficacy of doxycycline, which had an mic of 0.125 micrograms/ml. e. sennetsu was found to be resis ... | 1990 | 2221869 |
| molecular cloning of ehrlichia risticii and development of a gene probe for the diagnosis of potomac horse fever. | a gene bank of ehrlichia risticii was constructed in plasmid vector puc13. five clones representing discrete regions of the e. risticii genome were tested for their ability to hybridize specifically to e. risticii dna. none of the clones cross-hybridized with ehrlichia equi dna, whereas four of these clones cross-hybridized with ehrlichia canis and ehrlichia sennetsu dnas. however, one clone carrying a 1-kilobase hindiii fragment of e. risticii dna failed to cross-react with the genomes of e. se ... | 1990 | 2229378 |
| [interaction of local anesthetics with secretory cells at the membrane level]. | | 1985 | 2408715 |
| trypanosoma cruzi: cell type dependent distribution of a tubulin domain in mammalian stages. | the distribution of tubulin domains in the mammalian stages of trypanosoma cruzi was investigated by using monoclonal antibodies elicited against bovine brain tubulin. western blotting performed on t. brucei trypomastigotes and t. cruzi epimastigotes showed that the monoclonal antibodies 16d3 and 24e3 reacted only with tubulin in these cell types. indirect immunofluorescence revealed that, whereas 16d3 stained all microtubules, including subpellicular microtubules, the epitope defined by 24e3 wa ... | 1987 | 2443382 |
| primidone/phenobarbital-induced periodic alternating nystagmus. | a 37-year-old man with a history of seizures developed periodic alternating nystagmus (pan) along with other signs of primidone/phenobarbital toxicity. the pan gradually diminished in cycle length and intensity, finally resolving with gradual discontinuation of the drugs. | 1989 | 2764437 |
| reinfection of guinea pigs with herpes simplex virus type 2: failure to establish a second latent ganglionic infection. | guinea pigs were infected with herpes simplex virus type 2 (hsv 2) and at various times thereafter reinfected at a different site with the same or a different strain of hsv 2. viruses were isolated from infected tissues either from the site of primary or secondary infection or from regional ganglia. viral isolates were characterised by dna fingerprinting using restriction endonucleases capable of differentiating between the virus strains used. the second infection resulted in a second site of pe ... | 1987 | 2825951 |
| enzyme-linked immunosorbent assay for adherence of bacteria to animal cells. | epithelial cells scraped from human oral mucosa and from pig intestines were immobilized onto the flat bottom surfaces of microtiter plates to study the adherence of various bacterial species to host cells. bacterial adherence was quantitated either by an enzyme-linked immunosorbent assay technique with specific antibacterial serum as the first antibody followed by peroxidase-conjugated second antibody or by using biotinylated bacteria and avidin-peroxidase as the detecting agent. unlabeled esch ... | 1986 | 2877005 |
| involvement of postsynaptic adrenergic mechanism(s) in the atp-contractions of the rat anococcygeus muscle and the effect of extracellular ca2+. | clonidine, ici-106270 (6-aryl 2,367 tetrahydro 5-h-pyrrolo(1,2-a)-imidazole derivative), b-ht-920 (6-allyl-2-amino-5,6,7,8-tetrahydro-4h-thiozolo = [4,5-d]azepine-dihydrochloride, and guanfacine, agonists at alpha 2-adrenoceptors, produced concentration-dependent contractions of the rat anococcygeus muscle. the order of potency of these agonists in producing the response was clonidine greater than ici-106270 greater than guanfacine greater than b-ht 920. yohimbine (10(-6) m) competitively antago ... | 1988 | 2896487 |
| energy metabolism of monocytic ehrlichia. | we investigated if the monocytic ehrlichia are totally dependent on their host cells for energy, or, as rickettsia, are capable of some atp synthesis in vitro. the miyayama strain of ehrlichia sennetsu and the maryland and illinois strains of ehrlichia risticii were cultivated in a mouse macrophage cell line, separated from host cell constituents by procedures that included renografin or percoll gradient centrifugation, and tested after cryopreservation. cells incubated without a metabolizing su ... | 1989 | 2922404 |
| adaptation of ehrlichia sennetsu to canine blood monocytes: preliminary structural and serological studies with cell culture-derived ehrlichia sennetsu. | ehrlichia sennetsu, the causative agent of human sennetsu rickettsiosis, was successfully propagated in primary canine blood monocyte cultures. the growth cycle of this organism appears to be similar to that of ehrlichia canis. the antigen derived from our e. sennetsu cultures was used to develop an indirect fluorescent antibody test for detection and titration of serum antibodies to the organism. using this test system, we found that five human serum samples obtained from patients clinically di ... | 1985 | 2985504 |
| diabetic embryopathy and fuel-mediated organ teratogenesis: lessons from animal models. | the growing recognition that faulty maternal metabolism during early organogenesis may be implicated in the increased incidence of birth defects in pregnancies complicated by diabetes has prompted worldwide efforts to institute improved preconceptional metabolic regulation. however, the failure to identify the periods of greatest risk for diabetic embryopathy, the mediating teratogen(s), and the underlying mechanisms have complicated attempts to establish precise therapeutic guidelines and targe ... | 1988 | 3053387 |
| clinical, histopathological, and immunological responses of ponies to ehrlichia sennetsu and subsequent ehrlichia risticii challenge. | ehrlichia risticii has a close antigenic relationship to e. sennetsu. sera of ponies experimentally infected with e. risticii, the etiologic agent of potomac horse fever, consistently reacted with e. sennetsu, a human pathogen, in indirect fluorescent-antibody (ifa) testing, while human e. sennetsu convalescent serum reacted with e. risticii by ifa testing and immunoferritin labeling of cells infected in vitro. two ponies injected intravenously with live e. sennetsu did no develop clinical illne ... | 1988 | 3169993 |
| substrate utilization by ehrlichia sennetsu and ehrlichia risticii separated from host constituents by renografin gradient centrifugation. | the in vitro metabolic activities of two monocytic species of ehrlichia were investigated. the miyayama strain of ehrlichia sennetsu and two strains of ehrlichia risticii, isolated in illinois and maryland, were cultivated in a p388d1 mouse macrophage cell line. the ehrlichia particles from heavily infected cultures were separated from host constituents by a renografin gradient centrifugation procedure modified from those employed for rickettsiae and chlamydiae. the metabolic activities of the i ... | 1988 | 3182726 |
| clonorchiasis: an update. | the ability of clonorchis sinensis to cause severe hepatobiliary disease is illustrated by the case we have reported, in which biliary obstruction and hepatic fibrosis resulted from the pathologic process associated with chronic clonorchiasis. immunodiagnostic tests are currently limited, and diagnosis, as illustrated by our case, relies on identification of the fluke, usually as the adult or egg stage in clinical specimens. praziquantel has revolutionized therapy of this disease, but surgical d ... | 1987 | 3299730 |
| cytotoxic escherichia coli. | | 1987 | 3305326 |
| coordinative interactions in the separation of insulin and its derivatives by high-performance liquid chromatography. | the effects of various inorganic cations and anions on the chromatographic behaviour of insulin and its derivatives have been investigated. it was shown that on an octadecylsilica column at a given eluent composition the retention of insulins depends primarily on the nature of the anions. the cations have a secondary effect, depending on their size and coordination ability. the mechanism of the effect of the ions is complex. it includes the formation of ion pairs, their influence on dissociation ... | 1987 | 3323216 |
| evaluation of leptotrombidium (leptotrombidium) fletcheri (acari: trombiculidae) as a potential vector of ehrlichia sennetsu. | | 1987 | 3669025 |
| a comparison of the effects of climbing fiber deafferentation in adult and weanling rats. | the climbing fiber input to the cerebellar cortex was destroyed using both electrolytic and chemical (3-acetylpyridine) lesions. the long-term effects of climbing fiber deafferentation on the ansiform lobule of weanling and adult rats were examined at both the light and electron microscopic levels. image analysis of golgi-impregnated purkinje cells indicated a significantly lower number of smooth branches and spiny branchlets following climbing fiber deafferentation of both adult and weanling ra ... | 1986 | 3768690 |
| isolation, experimental transmission, and characterization of causative agent of potomac horse fever. | potomac horse fever, a disease characterized by fever, anorexia, leukopenia, and occasional diarrhea, is fatal in approximately 30 percent of affected animals. the seasonal occurrence of the disease (june to october) and evidence of antibodies to the rickettsia ehrlichia sennetsu in the serum of convalescing horses suggested that a related rickettsia might be the causative agent. such an agent was isolated in cultured blood monocytes from an experimentally infected pony. this intracytoplasmic or ... | 1985 | 3880925 |
| a light and electron microscopic examination of ehrlichia sennetsu in cultured human endothelial cells. | umbilical cord-derived human endothelial cells were used for the propagation of ehrlichia sennetsu. scanning electron microscopic studies revealed numerous pleomorphic ehrlichiae extending from the surface of infected human endothelial cells. transmission electron microscopic examination of the cells revealed e. sennetsu-induced cytopathic changes at the ultrastructural level. ehrlichiae of variable size, shape and density were located individually and in clusters enclosed within membrane lined ... | 1985 | 3913788 |
| splenectomy: an old issue of current controversy. | | 1986 | 3957756 |
| peritubular protein modulates neutral active nacl absorption in rabbit proximal convoluted tubule. | to assess directly the effect of increasing and decreasing peritubular protein concentration on active nacl absorption, rabbit proximal convoluted tubules were perfused with a high-chloride solution simulating late proximal tubular fluid and bathed in a similar solution containing 0, 6, or 10 g/dl albumin. a direct examination of the active component of nacl absorption was ensured by 1) the reduction of luminal solutes other than nacl (no glucose or alanine and only 5 mm hco-3), and 2) the use o ... | 1985 | 4003554 |
| continuous propagation of ehrlichia sennetsu in murine macrophage cell cultures. | ehrlichia sennetsu, the etiologic agent of human sennetsu rickettsiosis was successfully propagated in a continuous cell culture using murine cell lines p388d1 and raw 264. pleomorphic cytoplasmic inclusion bodies similar to ehrlichia canis morulae were observed 3-4 days after second post-inoculation split. in the raw 264 cell line e. sennetsu was not seen until the third passage. relatively heavier infection was observed in p388d1 than in raw cell line. the latter reached a maximum of 15% infec ... | 1985 | 4025693 |
| effects of cyclophosphamide on b- and t-lymphocytes in rheumatoid arthritis. | | 1974 | 4136699 |
| evidence for the participation of a cholinergic receptor in adrenergic transmission. | | 1970 | 4319312 |
| triphosphopyridine nucleotide photoreduction with cell-free preparations of anabaena variabilis. | | 1966 | 4381286 |
| effect of elemental diet on pancreatic secretion. | | 1974 | 4440810 |
| differentiation of salmon poisoning disease and elokomin fluke fever: fluorescent antibody studies with rickettsia sennetsu? | | 1973 | 4578540 |
| [experimental study of rheumatic carditis using hemolytic streptococcus type 12]. | | 1974 | 4615378 |
| [adaptory theory of protein biosynthesis]. | | 1971 | 4944706 |
| persistent infection of rickettsia sennetsu in cell culture system. | | 1965 | 5295095 |
| [study of infectious mononucleosis in kumamoto prefecture. 1. isolation of a rickettsia sennetsu-like organism from patients with epidemic glandular fever]. | | 1966 | 6013564 |
| [studies on the biological characteristics of rickettsia sennetsu. i. effect of temperature, ph and various disinfectants]. | | 1966 | 6013626 |
| [studies on the biological characteristics of rickettsia sennetsu. ii. sensitivity to drugs]. | | 1966 | 6013627 |
| rickettsia sennetsu in human blood monocyte cultures: similarities to the growth cycle of ehrlichia canis. | microscopic examination of cultured human monocytes infected with rickettsia sennetsu and stained by the giemsa method revealed the presence of various organismal growth forms in the cytoplasm of the infected cells. the growth forms were loosely scattered individual organisms, clusters of organisms, various sizes of dense inclusion bodies in intact and vacuolated cytoplasm, and organisms in close proximity to disintegrated monocytes. the appearance and the morphology of these r. sennetsu growth ... | 1982 | 6274798 |
| rickettsial diseases of the far east: new perspectives. | the rickettsial disease of man found only in asia is mite-borne (scrub) typhus, caused by rickettsia tsutsugamushi. unique to southern japan is a little-known human mononucleosis-like disease caused by rickettsia sennetsu. in 1981 and 1982, there was a remarkable resurgence in the number of reported cases of mite-borne typhus in japan after some years of virtual absence. recent studies of r sennetsu have resulted in its reclassification to the genus ehrlichia, members of which until now have bee ... | 1984 | 6425420 |
| evidence of a serologic relationship between ehrlichia canis and rickettsia sennetsu. | a serologic relationship between rickettsia sennetsu, the etiologic agent of human sennetsu rickettsiosis in western japan, and ehrlichia canis, the agent of canine ehrlichiosis, has been demonstrated. using the indirect fluorescent antibody test, convalescent sera from patients with sennetsu fever reacted with ehrlichia canis antigen. in the direct fluorescent antibody test, immunoglobulins from four patients with sennetsu rickettsiosis stained e. canis morulae in canine monocytes. this finding ... | 1981 | 7034563 |
| neorickettsia-like organism isolated from metacercaria of a fluke, stellantchasmus falcatus. | "hyuga" fever, reportedly caused by rickettsia sennetsu, occurring in the west of japan was suspected to be an endoparasite-borne rickettsiosis. no r. sennetsu, however, was detected in metacercariae found in the muscles of grey mullets (mugil cephalus) ingested raw by most "hyuga" fever patients. instead, another neorickettsia-like organism (sf strain) was isolated occasionally from metacercariae of stellantchasmus falcatus. a dog was inoculated with sf strain. it developed mild fever after an ... | 1981 | 7311106 |
| 16s rrna gene sequence of neorickettsia helminthoeca and its phylogenetic alignment with members of the genus ehrlichia. | neorickettsia helminthoeca (tribe ehrlichieae, family rickettsiaceae) is the agent of salmon poisoning disease, which affects members of the family canidae. this bacterium is unusual in that it is the only known obligately intracellular bacterium that is transmitted via a helminth vector. the nucleotide sequence of the n. helminthoeca 16s rrna gene was determined and compared with the sequences of intracellular bacteria belonging to the alpha subgroup of the proteobacteria. the n. helminthoeca s ... | 1995 | 7537055 |
| diversity of 16s rrna genes of new ehrlichia strains isolated from horses with clinical signs of potomac horse fever. | ehrlichia risticii is the causative agent of potomac horse fever. variations among the major antigens of different local e. risticii strains have been detected previously. to further assess genetic variability in this species or species complex, the sequences of the 16s rrna genes of several isolates obtained from sick horses diagnosed as having potomac horse fever were determined. the sequences of six isolates obtained from ohio and three isolates obtained from kentucky were amplified by pcr. t ... | 1995 | 7537065 |
| serologic cross-reactions among ehrlichia equi, ehrlichia phagocytophila, and human granulocytic ehrlichia. | homology in the 16s rdnas shows that the agent of human granulocytic ehrlichiosis (hge) is closely related to the veterinary pathogens erlichia equi and erlichia phagocytophila. after hge, patients develop antibodies reactive with e. equi and e. phagocytophila; thus, we hypothesized that these species are closely related and share significant antigenicity. antisera from humans, horses, dogs, and cattle were tested by indirect fluorescent-antibody assay (ifa) for antibodies reactive with e. equi ... | 1995 | 7542262 |
| cytopathic effect, plaque formation, and lysis of ehrlichia chaffeensis grown on continuous cell lines. | ehrlichiae are strict intracellular bacterial pathogens that parasitize leukocytes or other blood cells. only six agents of the tribe ehrlichieae, namely, cowdria ruminantium, neorickettsia helminthoeca, ehrlichia risticii, ehrlichia sennetsu, ehrlichia canis, and ehrlichia chaffeensis, have been adapted to growth in continuous cell lines. e. chaffeensis, the agent of human ehrlichiosis, has been cultured only in a cell line of canine origin. we adapted purified cell-free e. chaffeensis for grow ... | 1994 | 8300201 |
| characterization of ehrlichial organisms isolated from a wild mouse. | an infectious agent was isolated from the enlarged spleen of a wild mouse, eothenomys kageus, by intraperitoneal inoculation of the spleen homogenate into laboratory mice. the laboratory mice developed splenomegaly, and the agent was maintained by serial passage of spleen homogenates in laboratory mice. the agent in the spleen homogenate was inactivated after incubation at 37 or 50 degrees c. tetracyclines were effective in preventing infection of mice with this agent, but penicillin and sulfona ... | 1993 | 8380184 |
| characterization of the sf agent, an ehrlichia sp. isolated from the fluke stellantchasmus falcatus, by 16s rrna base sequence, serological, and morphological analyses. | the organism designated the sf agent was originally isolated in japan in 1962 from stellantchasmus falcatus metacercaria parasitic on gray mullet fish. the sf agent resembles members of the genus ehrlichia morphologically and exhibits weak antigenic cross-reactivity with ehrlichia sennetsu. this organism causes mild clinical signs in dogs, but severe splenomegaly and lymphadenopathy in mice. this suggests that the sf agent may be similar to either neorickettsia helminthoeca, an intracellular par ... | 1996 | 8573488 |
| ehrlichia sennetsu groe operon and antigenic properties of the groel homolog. | a clone expressing an immunoreactive 55-kilodalton (kda) protein of ehrlichia sennetsu, the causative agent of human sennetsu ehrlichiosis, was isolated from a gene library of this organism. sequence analysis of the dna insert revealed two open reading frames, encoding proteins of 10,620 and 58,225 kda, respectively. these deduced amino acid sequences were homologous to those of the groes and groel heat shock proteins (hsp) of other bacteria, respectively. phylogenetic trees based on groes and g ... | 1997 | 9215585 |
| cloning of the heat shock protein 70 (hsp70) gene of ehrlichia sennetsu and differential expression of hsp70 and hsp60 mrna after temperature upshift. | ehrlichia sennetsu is the causative agent of human sennetsu ehrlichiosis. heat shock protein 60 (hsp60) and hsp70 (dnak) are two major bacterial hsps, and their interaction modulates the stress response. previously, we cloned and sequenced groe and expressed groel of e. sennetsu. hsp60 (groel) was immunogenic and cross-reactive in ehrlichia spp. the present study was designed to (i) characterize the hsp70 gene of this organism and (ii) determine whether the expression of these two hsps is induci ... | 1998 | 9632573 |
| characterization of monoclonal antibodies to the 44-kilodalton major outer membrane protein of the human granulocytic ehrlichiosis agent. | the major outer membrane proteins (omps) of the human granulocytic ehrlichiosis (hge) agent, with molecular sizes of 44 to 47 kda, are immunodominant antigens in human infection. monoclonal antibodies (mabs) to the omps were made by immunizing balb/c mice with the purified hge agent and then by fusing spleen cells with myeloma cells. the immunologic specificities of three mabs (3e65, 5c11, and 5d13) were examined with five human hge agent isolates and one tick isolate. by western blot analysis, ... | 1998 | 9774579 |
| [an update on human ehrlichiosis]. | human ehrlichiosis is a potentially lethal infections due to a new gram-negative microorganism. the first human case was reported in 1953 in japan. two of the animal agents are involved in human disease: ehrlichia chaffeensis, ehrlichia sennetsu. a third ehrlichia type agent causes the disease in human beings only--ehrlichia granulocytophila. the tick is natural vector; the highest frequency was observed in may-july, in the united states; the most important clinical signs are: fever, anorexia, r ... | 1998 | 10756846 |
| dna gyrase-mediated natural resistance to fluoroquinolones in ehrlichia spp. | fluoroquinolone susceptibility heterogeneity between various ehrlichia species has been previously demonstrated. in gram-negative bacteria, resistance to fluoroquinolones most often corresponds to specific amino acid variations in a portion of the protein sequence of the a subunit of dna gyrase (gyra), referred to as the quinolone resistance-determining region (qrdr). we suspected a similar mechanism to be responsible for natural resistance in some ehrlichia species. to verify this hypothesis, w ... | 2001 | 11408229 |
| phylogenetic relationships of anaplasma marginale and 'ehrlichia platys' to other ehrlichia species determined by groel amino acid sequences. | the heat-shock protein (groel) genes of anaplasma marginale, ehrlichia muris and 'ehrlichia platys' were sequenced and compared with the groel of other species of ehrlichia. the groel amino acid sequences of a. marginale and 'e. platys' were most similar to the groel sequence of ehrlichia phagocytophila, with which they formed one group with 6-10% divergence. the e. muris groel was most closely related to the groel of two unclassified strains (hf-565 and anan), then to ehrlichia chaffeensis, ehr ... | 2001 | 11414267 |
| citrate synthase gene sequence: a new tool for phylogenetic analysis and identification of ehrlichia. | the sequence of the citrate synthase gene (glta) of 13 ehrlichial species (ehrlichia chaffeensis, ehrlichia canis, ehrlichia muris, an ehrlichia species recently detected from ixodes ovatus, cowdria ruminantium, ehrlichia phagocytophila, ehrlichia equi, the human granulocytic ehrlichiosis [hge] agent, anaplasma marginale, anaplasma centrale, ehrlichia sennetsu, ehrlichia risticii, and neorickettsia helminthoeca) have been determined by degenerate pcr and the genome walker method. the ehrlichial ... | 2001 | 11526124 |
| reorganization of genera in the families rickettsiaceae and anaplasmataceae in the order rickettsiales: unification of some species of ehrlichia with anaplasma, cowdria with ehrlichia and ehrlichia with neorickettsia, descriptions of six new species combinations and designation of ehrlichia equi and 'hge agent' as subjective synonyms of ehrlichia phagocytophila. | the genera anaplasma, ehrlichia, cowdria, neorickettsia and wolbachia encompass a group of obligate intracellular bacteria that reside in vacuoles of eukaryotic cells and were previously placed in taxa based upon morphological, ecological, epidemiological and clinical characteristics. recent genetic analyses of 16s rrna genes, groesl and surface protein genes have indicated that the existing taxa designations are flawed. all 16s rrna gene and groesl sequences deposited in genbank prior to 2000 a ... | 2001 | 11760958 |
| rna polymerase beta-subunit-based phylogeny of ehrlichia spp., anaplasma spp., neorickettsia spp. and wolbachia pipientis. | sequence analysis of rpob, the gene encoding the beta-subunit of rna polymerase, was used in a phylogenetic investigation of nine species from the genera ehrlichia, neorickettsia, wolbachia and anaplasma. the complete nucleotide sequences obtained for anaplasma phagocytophilum (hge agent), ehrlichia chaffeensis, neorickettsia sennetsu, neorickettsia risticii, anaplasma marginale and wolbachia pipientis were amongst the longest rpob sequences in genbank and ranged from 4074 bp for n. sennetsu to ... | 2003 | 12710612 |
| mechanisms to create a safe haven by members of the family anaplasmataceae. | members of the family anaplasmataceae are obligatory intracellular bacteria with unique host cell specificities. depending on each bacterial species, granulocytes, platelets, endothelial cells, monocytes, macrophages, red blood cells, and cells of invertebrates are specifically infected. this unique host cell specificity has been the major hurdle to overcome in order to cultivate this group of bacteria. because these bacteria cannot survive outside host cells, once released from a host cell, the ... | 2003 | 12860688 |
| persistent infection of fl cells by rickettsia sennetsu (s. todai). | | 1964 | 14222807 |
| studies on infectious mononucleosis induced in the monkey by experimental infection with rickettsia sennetsu. ii. pathological findings. | | 1965 | 14319489 |
| studies on infectious mononucleosis induced in the monkey by experimental infection with rickettsia sennetsu. i. clinical observations and etiological investigations. | | 1965 | 14322603 |
| analysis of p51, groesl, and the major antigen p51 in various species of neorickettsia, an obligatory intracellular bacterium that infects trematodes and mammals. | the p51 gene that encodes the major antigenic 51-kda protein in neorickettsia risticii was identified in strains of neorickettsia sennetsu and the stellantchasmus falcatus agent but not in neorickettsia helminthoeca, suggesting that p51-based diagnosis would be useful to distinguish among them. groesl sequencing results delineated the phylogenic relationships among neorickettsia spp. | 2004 | 15297539 |
| comparative genomics of emerging human ehrlichiosis agents. | anaplasma (formerly ehrlichia) phagocytophilum, ehrlichia chaffeensis, and neorickettsia (formerly ehrlichia) sennetsu are intracellular vector-borne pathogens that cause human ehrlichiosis, an emerging infectious disease. we present the complete genome sequences of these organisms along with comparisons to other organisms in the rickettsiales order. ehrlichia spp. and anaplasma spp. display a unique large expansion of immunodominant outer membrane proteins facilitating antigenic variation. all ... | 2006 | 16482227 |
| budget genome. | | 2006 | 16722005 |
| sennetsu neorickettsiosis: a potentially prevalent, treatable, acutely incapacitating tropical infectious disease. | | 2009 | 19635866 |
| sennetsu neorickettsiosis: a probable fish-borne cause of fever rediscovered in laos. | neorickettsia sennetsu has been described from japan and malaysia, causing a largely forgotten infectious mononucleosis-like disease. because it is believed to be contracted from eating raw fish, frequently consumed in the lao pdr, we looked for evidence of n. sennetsu among lao patients and fish. a buffy coat from 1 of 91 patients with undifferentiated fever was positive by 16s rrna amplification and sequencing and real-time polymerase chain reactions (pcr) targeting two n. sennetsu genes. lao ... | 2009 | 19635868 |
| [ehrlichiosis/anaplasmosis]. | ehrlichiosis and anaplasmosis are zoonoses caused by bacteria from the family anaplasmataceae, including human and animal pathogens. the human pathogens are ehrlichia chaffeensis, the causative agent of human monocytic ehrlichiosis (hme), anaplasma phagocytophilum, the pathogen causing human granulocytic anaplasmosis (hga), e. ewingii and neorickettsia sennetsu, granulocytotropic and monocytotropic ehrlichia species, respectively. ehrlichia spp. are small, gram-negative, obligate intracellular b ... | 2009 | 20077398 |
| proteomic analysis of neorickettsia sennetsu surface-exposed proteins and porin activity of the major surface protein p51. | neorickettsia sennetsu is an obligate intracellular bacterium of monocytes and macrophages and is the etiologic agent of human sennetsu neorickettsiosis. neorickettsia proteins expressed in mammalian host cells, including the surface proteins of neorickettsia spp., have not been defined. in this paper, we isolated surface-exposed proteins from n. sennetsu by biotin surface labeling followed by streptavidin-affinity chromatography. forty-two of the total of 936 (4.5%) n. sennetsu open reading fra ... | 2010 | 20833807 |
| legionella pneumophila secretes a mitochondrial carrier protein during infection. | the mitochondrial carrier family (mcf) is a signature group of integral membrane proteins that transport metabolites across the mitochondrial inner membrane in eukaryotes. mcf proteins are characterized by six transmembrane segments that assemble to form a highly-selective channel for metabolite transport. we discovered a novel mcf member, termed legionellanucleotide carrier protein (lncp), encoded in the genome of legionella pneumophila, the causative agent of legionnaire's disease. lncp was se ... | 2012 | 22241989 |
| when is serum albumin worth measuring? | | 2011 | 3214132 |