| an electromyographic study of uterotubal activity in the ewe. | the electrical activity of the oviduct and uterus was automatically registered on a potentiometric pen recorder in ewes fitted with an extracellular multielectrode assembly. the duration and amplitude of local non-propagating activity of the uterus increased and became progressively grouped into phases as natural oestrus progressed. phases of contractions lasting 5-6 min were initially propagated at a rate of 2/hr. their frequency increased within 24 hr of the decline in plasma progesterone leve ... | 1976 | 60491 |
| guinea pig transfer factor: restriction of macrophage migration inhibitory activity to extraordinary dilution confirmed with demonstration of diminished potency of stored preparations. | | 1979 | 88292 |
| ultrastructural localization of calcium around the membrane of the surface connected system in the human platelet. | the localization of calcium in the membrane system of human platelets was determined by ultrahistochemical methods equipped with an electron probe x-ray microanalyzer. after potassium oxalate-glutaraldehyde treatment large amounts of electron opaque precipitates were observed around the membrane of the surface connected system. electron probe x-ray microanalysis clearly defined that the precipitates were composed of calcium oxalate. the localization of calcium on the membrane of the surface conn ... | 1978 | 96043 |
| transfer of 2,4,5,2',4',5'-hexachlorobiphenyl and 2,2-bis-(p-chlorophenyl),1,1,1-trichloroethane (p,p'-ddt) from maternal to newborn and suckling rats. | pregnant rats were given a small dose of 14c-2,4,5,2',4',5'-hexachlorobiphenyl (hcb) and 3h-ddt intraperitoneally. the transfer of hcb and ddt through the placenta and milk was then investigated. transfer through the placenta was 2.7 and 1.5% (respectively) of the initial doses; transfer through milk was 39.2 and 21.5%. hcb is obviously more transferable than ddt through the placenta and milk, the ratio of the amount of hcb transferred through milk to the amount transferred through the placenta ... | 1978 | 104695 |
| [immunocytological study of the gonadotrophic and thyrotrophic cells of the rat adenohypophysis]. | on paraffin or semi-thin sections various anti-lh or anti-tsh sera stain indifferently all the thyrotrophs and the gonadotrophs. inversely anti-beta-tsh, anti-beta-lh or anti-beta-fsh purified sera permit the discrimination of these two cell populations. the constancy of fixation of the anti-beta-lh and anti-beta-fsh sera on all the gonadotrophs gives evidence of their ability to produce both fsh and lh. however in a few female rats the central gonadotrophs are stained more weakly by anti-beta-f ... | 1977 | 145301 |
| [intravenous cholangiography in hyperbilirubinaemia (author's transl)]. | the effect of bilirubin on biliary and renal excretion of methylglucamine-iodipamide at an infusion rate of 1.3, 2.6 and 5.2 mu mol/min/kg was studied by intravenous injection into five dogs with normal liver functions and chronic duodenal fistulas. unconjugated bilirubin administered in a dose of 0.1 mu mol/min/kg reduced biliary iodipamide excretion and concentration significantly, but did not affect renal contrast material excretion. this indicates that the unsatisficatory radiological result ... | 1979 | 153309 |
| structural studies and physical maps for the herpes simplex virus genome. | | 1977 | 193820 |
| altered coupling states between calcium transport and (ca2+, mg2+)-atpase in the as-30d ascites hepatocarcinoma plasma membrane. | plasma membrane fractions from normal, regenerating liver and the as-30d ascites hepatocarcinoma exhibited a high degree of enrichment when a set of plasma membrane enzyme markers were studied in comparison to the ones associated to the mitochondrial and cytosolic compartments. while the (ca2+, mg2+)-atpase observed for the plasma membrane fraction isolated from normal liver showed an activity of 1.2 mumoles/mg/min, the regenerating liver and the as-30d plasma membrane fractions presented a much ... | 1991 | 1828860 |
| lovastatin inhibits gallstone formation in the cholesterol-fed prairie dog. | the efficacy of lovastatin, an inhibitor of hepatic cholesterol synthesis in the prevention of cholesterol gallstone formation, was evaluated in the prairie dog model. two groups of animals were maintained on either nonlithogenic or 1.2% cholesterol-enriched chow for 21 days. seven of the animals in each group received lovastatin, and the remaining six received only distilled water. all of the cholesterol-fed/water-treated animals had crystals and 83% had gallstones, but none of the cholesterol- ... | 1991 | 1867522 |
| immunocytochemical detection of ehrlichia platys antigens in canine blood platelets. | an avidin-biotin immunoperoxidase complex (abc) immunocytochemical (icc) stain procedure was optimized for detection of ehrlichia platys antigens. positive immunoreactivity was detected with dilutions of canine immune serum on acetone-fixed smears of platelet-rich plasma from e. platys-infected dogs. no e. platys antigens were detected when this icc stain was applied to frozen or paraffin-embedded formalin- or acetone-fixed tissue sections from dogs with acute e. platys infection. acetone fixati ... | 1991 | 1911994 |
| evaluation of rhipicephalus sanguineus as a potential biologic vector of ehrlichia platys. | the brown dog tick, rhipicephalus sanguineus (acari: ixodidae), transmits several diseases among dogs including ehrlichia canis infection. the role of rhipicephalus sanguineus as a biologic vector for e platys, the rickettsial agent of infectious canine cyclic thrombocytopenia, was studied in dogs. laboratory-cultured, pathogen-free nymph ticks were fed to repletion on dogs acutely infected with e platys. tick engorgement coincided with the development of initial parasitemia and thrombocytopenia ... | 1991 | 1952347 |
| ehrlichial diseases of dogs. | ehrlichial diseases of dogs currently are recognized to be caused by ehrlichia canis, ehrlichia platys, ehrlichia equi, and ehrlichia risticii. information on each disease's history, transmission, pathogenesis, clinicopathologic findings, diagnosis, therapy, and prevention is presented in this article. ehrlichia canis is the most common cause of clinical illness. case reports are included to illustrate common presentations of ehrlichia canis infections. | 1991 | 2014630 |
| platelet aggregation studies in dogs with acute ehrlichia platys infection. | ten adult dogs (5 beagles and 5 mixed-breed dogs) were inoculated iv with canine platelets containing ehrlichia platys. inclusions and morulae of e platys developed in platelets of infected dogs at 10 to 14 days after inoculation, followed by marked thrombocytopenia at 14 to 21 days. parasitemia and marked thrombocytopenia recurred at 24 to 28 days after inoculation. increased numbers of megakaryocytes were observed in marrow aspirate smears from infected dogs, indicative of regenerative thrombo ... | 1990 | 2301842 |
| mechanisms and kinetics for platelet and neutrophil localization in immune complex nephritis. | we have previously reported that both neutrophils (pmns) and platelets mediate proteinuria in a model of subendothelial immune complex (ic) nephritis (gn) in the rat. in order to understand the interaction of pmns and platelets in this model, we quantitated the uptake of 111in-labelled platelets in glomeruli and correlated this with the number of pmns observed histologically at 10 and 30 minutes, 1, 4 and 24 hours following induction of gn. platelet accumulation was biphasic with a major peak at ... | 1989 | 2533296 |
| similarities and differences in the regulation of hepatic cytochrome p-450 enzymes by diabetes and fasting in male rats. | the effects of streptozotocin-induced diabetes and fasting on hepatic cytochrome p-450 enzymes in sexually mature male rats were studied by immunochemical techniques and enzyme assays. the level of cytochrome p-450ac (an acetone/ethanol inducible form), 65 pmol/mg microsomal protein in control rats, increased 4- to 5-fold in diabetic rats and 3- and 5-fold in fasting rats. in contrast, p-450 ut-a (a male specific form) decreased drastically from 295 pmol/mg in the control group to about 10% of t ... | 1989 | 2818618 |
| substrate specificity to maintain cellular atp along the mouse nephron. | to evaluate substrate utilization along the mouse nephron, cellular atp content was measured under various conditions by the luciferin-luciferase technique. individual micro-dissected nephron segments were incubated in a modified hanks' solution (ph 7.4) with or without each of the following substrates: d-glucose, dl-lactate, beta-hydroxybutyrate (hba), and l-glutamine. atp production from glucose was minimal in the early proximal tubule (s1), but was substantial in the late proximal tubule (s3) ... | 1988 | 2847554 |
| structural bases of the cytolytic mechanisms of entamoeba histolytica. | the cellular bases of the powerful cytolytic activity of the human protozoan parasite entamoeba histolytica were explored by studying the effect of the virulent strain hm1:imss on epithelial monolayers of mdck cells using a combination of time-lapse microcinematography and transmission and scanning electron microscopy. early alterations of the epithelial cell membranes were detected by measuring changes in the transepithelial electrical resistance of mdck monolayers mounted in ussing chambers. t ... | 1985 | 2859368 |
| [heterogeneity of purinergic p2 receptors at the level of the caudal artery of the rat and the saphenous vein of the dog]. | phentolamine (10(-5) m) and an inhibitor of the lipoxygenase pathway, nordihydroguaiaretic acid (n. d. g. a.; 8 10(-6) m) antagonized the atp induced contraction but not antagonized the utp induced contraction on both rat tail artery and dog saphenous vein. we conclude that the receptors to atp are distinct from receptors to utp and that the p2 purinoceptors are an heterogeneous group. | 1987 | 2957036 |
| binding characteristics of swine erythrocyte insulin receptors. | crossbred gilts (controls; n = 7) had 8.8 +/- 1.1% (mean +/- sem) maximum binding of [125i]insulin to insulin receptors on erythrocytes. the number of insulin-binding sites per cell was 137 +/- 19, with a binding affinity ranging from 7.4 x 10(7)m-1 to 11.2 x 10(7)m-1 and mean of 8.8 x 10(7)m-1. pregnant sows (n = 5) had a significant increase (p less than 0.01) in maximum binding due to an increase in number of receptor sites per cell. lactating sows fed a high-fiber diet (n = 3) and a low-fibe ... | 1985 | 2996389 |
| effect of thyroid status and paraventricular area lesions on the release of thyrotropin-releasing hormone and catecholamines into hypophysial portal blood. | trh is a potent stimulator of pituitary tsh release, but its function in the physiological regulation of thyroid activity is still controversial. the purpose of the present study was to investigate trh and catecholamine secretion into hypophysial portal blood of hypothyroid and hyperthyroid rats, and in rats bearing paraventricular area lesions. male rats were made hypothyroid with methimazole (0.05% in drinking water) or hyperthyroid by daily injections with t4 (10 micrograms/100 g bw). untreat ... | 1988 | 3133200 |
| antibodies to ehrlichia canis, ehrlichia platys, and spotted fever group rickettsiae in louisiana dogs. | antibodies to ehrlichia canis, ehrlichia platys, and spotted fever group (sfg) rickettsiae were detected by indirect immunofluorescence in sera from 27 ill individually owned thrombocytopenic dogs (platelet concentrations less than 200,000 platelets/microliters) and 59 healthy kenneled dogs located in southern louisiana. platelet concentrations less than 100,000 platelets/microliters were detected in 63% of ill thrombocytopenic dogs and 6.8% of healthy kennel dogs. one ill thrombocytopenic dog h ... | 1988 | 3146636 |
| relaxin and progesterone secretion as affected by luteinizing hormone and prolactin after hysterectomy in the pig. | plasma levels of relaxin and progesterone in hysterectomized and pregnant gilts were determined from days 100-120 to evaluate the effects of purified porcine (p) lh and pprl on the secretory activity of the aging corpora lutea. gilts were bred on the second observed estrus or were hysterectomized between 6-8 days after estrus (estrus = day 0) and were assigned randomly to one of three treatment groups; saline-treated control, im injections of plh, and iv injections of pprl from days 110-120. in ... | 1988 | 3359961 |
| relaxin and progesterone secretion as affected by luteinizing hormone and prolactin after hysterectomy in the pig. | plasma levels of relaxin and progesterone in hysterectomized and pregnant gilts were determined from days 100-120 to evaluate the effects of purified porcine (p) lh and pprl on the secretory activity of the aging corpora lutea. gilts were bred on the second observed estrus or were hysterectomized between 6-8 days after estrus (estrus = day 0) and were assigned randomly to one of three treatment groups; saline-treated control, im injections of plh, and iv injections of pprl from days 110-120. in ... | 1988 | 3359961 |
| fatal respiratory disease in nilgiri tahr: possibly malignant catarrhal fever. | nilgiri tahr (hemitragus hylocrius) are native to india and are a rare zoo exhibit. this report describes an acute respiratory disease in tahr that caused the death of 15 of 16 animals in an extensive exhibit of about 35 acres where they were housed together with a variety of other exotic species of ruminants. the deaths occurred in two separate outbreaks and were associated with losses from malignant catarrhal fever in other ruminants in the exhibit. the most prominent clinical sign was severe ... | 1988 | 3370557 |
| anemia of inflammation in dogs infected with ehrlichia platys. | ten adult male dogs were inoculated with ehrlichia platys, and blood samples were collected throughout the infection to evaluate the hematologic changes with respect to serum biochemical analytes. all dogs developed a mild, normocytic, normochromic anemia by postinoculation day 7, with significantly (p less than 0.05) decreased serum iron concentration and total iron-binding capacity. stainable bone marrow iron appeared normal or increased throughout the infection. by postinoculation day 31, the ... | 1988 | 3421524 |
| epidermal growth factor stimulates (pro-)insulin biosynthesis and 3h-thymidine incorporation in isolated pancreatic rat islets. | | 1986 | 3546050 |
| acute ehrlichia platys infection in the dog. | ten dogs were inoculated with ehrlichia platys (e. platys) from an acutely infected dog. two dogs were necropsied on each of days 7, 14, 21, 28, and 35 post-inoculation, and tissues were collected and either fixed in formalin or frozen for light microscopic examination of lesions or e. platys antigen localization in tissues. serum antibody titers to e. platys and serum aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase activities were also determined. the significant ... | 1987 | 3672811 |
| a comparison of the effects of climbing fiber deafferentation in adult and weanling rats. | the climbing fiber input to the cerebellar cortex was destroyed using both electrolytic and chemical (3-acetylpyridine) lesions. the long-term effects of climbing fiber deafferentation on the ansiform lobule of weanling and adult rats were examined at both the light and electron microscopic levels. image analysis of golgi-impregnated purkinje cells indicated a significantly lower number of smooth branches and spiny branchlets following climbing fiber deafferentation of both adult and weanling ra ... | 1986 | 3768690 |
| uveitis associated with ehrlichia platys infection in a dog. | bilateral anterior uveitis, nonregenerative anemia, and thrombocytopenia were found in a 9-month-old chow chow infected with ehrlichia platys. diagnosis was based on the finding of basophilic inclusions in platelets and an e platys antibody titer. the dog was treated orally with tetracycline, topically with antibiotic/corticosteroid, and topically with a mydriatic. | 1986 | 3771363 |
| new model systems to study dna-protein recognition mechanisms. | dpg antibodies were fractionated on a cellulose-double-stranded dna column. the flow-through fraction bound denatured dna but not double-stranded dna (dsdna). the dpg-eluted fraction bound dsdna preferentially. the results show that proteins can recognise dpg in dna by different mechanisms, some involving dna unwinding. | 1985 | 3896854 |
| the structure of the lactose permease derived from raman spectroscopy and prediction methods. | the secondary structure of the lactose permease of escherichia coli reconstituted in lipid membranes was determined by raman spectroscopy. the alpha-helix content is approximately 70%, the beta-strand content below 10% and beta-turns contribute 15%. about 1/3 of the residues in alpha-helices and most other residues are exposed to water. employing a method for structural prediction which accounts for amphipathic helices, 10 membrane-spanning helices are predicted which are either hydrophobic or a ... | 1985 | 3912174 |
| primary renal candidiasis. | | 1985 | 3984964 |
| activation of branched-chain alpha-ketoacid dehydrogenase complex by alpha-chloroisocaproate in normal and enzyme-deficient fibroblasts. | a method has been developed for the activation of the branched-chain alpha-ketoacid dehydrogenase complex by alpha-chloroisocaproate, an inhibitor of branched-chain alpha-ketoacid dehydrogenase kinase in human cultured skin fibroblasts. the enzyme could be activated by pretreating the cells with alpha-chloroisocaproate before they were disrupted for measurement of the activity. after this treatment, the activity was 2- to 3-fold that of untreated cells (24.8-81.4 pmol/min per mg protein). the en ... | 1985 | 3987057 |
| peritubular protein modulates neutral active nacl absorption in rabbit proximal convoluted tubule. | to assess directly the effect of increasing and decreasing peritubular protein concentration on active nacl absorption, rabbit proximal convoluted tubules were perfused with a high-chloride solution simulating late proximal tubular fluid and bathed in a similar solution containing 0, 6, or 10 g/dl albumin. a direct examination of the active component of nacl absorption was ensured by 1) the reduction of luminal solutes other than nacl (no glucose or alanine and only 5 mm hco-3), and 2) the use o ... | 1985 | 4003554 |
| immunization and immunosuppression in mice reared for high or low immune responsiveness against nematospiroides dubius. | | 1985 | 4030203 |
| the chemistry of allergens. xxi. eight new antigens generated by successive pepsin hydrolyses of bovine -lactoglobulin. | | 1972 | 4114295 |
| increased diamine oxidase activity in the intestinal lymph of rats on fat ingestion. | | 1974 | 4212082 |
| [mechanism of the positive ionotropic action of theophylline on the mamalian heart. i. influence of extracellular ca, na and k concentrations and of reserpine]. | | 1971 | 4257640 |
| [the influence of angiotensin on the glucose blood level in rabbits]. | | 1968 | 4302712 |
| microbiological aspects of radiopasteurized chicken. nyo-3733-6. | | 1969 | 4313244 |
| binding of spin-labeled local anesthetics to phosphatidylcholine and phosphatidylserine liposomes. | | 1974 | 4369807 |
| closed circular dnas with tandem repeats of a sequence from polyoma virus. | | 1974 | 4369867 |
| studies on amino acid utilization in relation to virulence of macrophomina phaseoli causing disease of jute (corchorus capsularis l.). | | 1974 | 4407383 |
| the flank approach to feline ovariohysterectomy (an alternate technique). | | 1974 | 4493822 |
| effect of na+ on ca-uptake of the synaptic plasma membrane. | | 1974 | 4549937 |
| [transport of peroxidase in the epithelium of the rat intestine]. | | 1972 | 4638295 |
| pentobarbital facilitated extinction: effects of different schedules of drug withdrawal. | | 1974 | 4858098 |
| the calculated nitrogen requirements of a single cell of escherichia coli. | | 1968 | 4869285 |
| canine infectious cyclic thrombocytopenia found in taiwan. | here were report the first canine infectious cyclic thrombocytopenia (cict) found in taiwan. platelet-specific inclusions were detected in the blood smear of a military working dog. to identify the etiologic agent, the patient's blood was transmitted to three six-month-old german shepherd dogs. the ehrlichia platys-like inclusions were observed six to eight days after inoculation. indirect fluorescent antibody test showed that the serum from the patient reacted specifically with the microorganis ... | 1996 | 8741613 |
| ehrlichia platys infection in dogs. | | 1996 | 8905592 |
| specific amplification of ehrlichia platys dna from blood specimens by two-step pcr. | a two-step pcr method for diagnosis of canine infectious cyclic thrombocytopenia was established. three primers derived from the 16s and rrna gene sequence were used to amplify genomic dna specifically from ehrlichia platys. two-round amplification with dna templates prepared from e. platys-infected blood specimens produced 742- and 385-bp fragments, but these products were not found when an ehrlichia canis-infected blood sample and escherichia coli were used. this method, for which the minimum ... | 1996 | 8940461 |
| characterization of a new isolate of ehrlichia platys (order rickettsiales) using electron microscopy and polymerase chain reaction. | a mixed-breed pup approximately 3 months old obtained in north central oklahoma by the laboratory animal resources unit of oklahoma state university presented with platelet inclusions. the dog developed severe thrombocytopenia (< 10,000 microliters-1) following the appearance of inclusions. blood films were monitored daily and when about 75% of platelets had inclusions, samples were collected in edta and processed for electron microscopic (em) studies and polymerase chain reaction (pcr). em stud ... | 1997 | 9066046 |
| different organisms associated with heartwater as shown by analysis of 16s ribosomal rna gene sequences. | cowdria ruminantium is a rickettsial parasite which causes heartwater, a economically important disease of domestic and wild ruminants in tropical and subtropical africa and parts of the caribbean. because existing diagnostic methods are unreliable, we investigated the small-subunit ribosomal rna (srrna) gene from heartwater-infected material to characterise the organisms present and to develop specific oligonucleotide probes for polymerase chain reaction (pcr) based diagnosis. dna was obtained ... | 1997 | 9299697 |
| clinical manifestations of infectious canine cyclic thrombocytopenia. | this paper describes five naturally occurring clinical cases of infectious canine cyclic thrombocytopenia that were the first serologically confirmed cases of ehrlichia platys infection in israel. in the usa this disease is considered subclinical, but the dogs in this study developed distinct clinical abnormalities. the signs observed by the owners included anorexia, lethargy, depression, weight loss and a mucopurulent nasal discharge. the principal findings on physical examination included lymp ... | 1997 | 9308149 |
| two-step pcr in the evaluation of antibiotic treatment for ehrlichia platys infection. | we evaluated the feasibility of using the two-step polymerase chain reaction (pcr) in determining the withdrawal time of antibiotic treatment for ehrlichia platys infection. we also present experimental evidence of a dog remaining a carrier after treatment with tetracycline. canine infectious cyclic thrombocytopenia (cict) was induced in 3 dogs by intravenous inoculation of blood infected with e. platys. tetracycline was administered to one of the dogs for 2 weeks when parasitemia appeared. alth ... | 1997 | 9342716 |
| [imported arthropod-borne parasites and parasitic arthropods in dogs. species spectrum and epidemiologic analysis of the cases diagnosed in 1995/96]. | between january 1995 and december 1996 nonendemic or only regionally occurring arthropodborne parasites including ehrlichiae and parasitic arthropods in germany were detected in 484 dogs, whereby at least 15 species were involved. listed in decreasing order, leishmania infections occurred most frequently, followed by infections/infestations with babesia canis, ehrlichia canis, rhipicephalus sanguineus, dirofilaria immitis and dermacentor reticulatus. the other species, namely babesia gibsoni, tr ... | 1998 | 9531673 |
| morphological evidence for infection of impala, aepyceros melampus, platelets by a rickettsia-like organism. | ultrastructural studies revealed the presence of a parasite, believed to be ehrlichia platys, in the blood platelets of impala. at the time of blood sampling all the animals appeared healthy. this is the first report on the presence of this rickettsia in these animals, previously described in canine platelets. | 1997 | 9551485 |
| coinfection with multiple tick-borne pathogens in a walker hound kennel in north carolina. | both dogs and humans can be coinfected with various ehrlichia, bartonella, rickettsia, and babesia species. we investigated a kennel of sick walker hounds and their owners in southeastern north carolina for evidence of tick-borne infections and associated risk factors. a high degree of coinfection was documented in the dog population. of the 27 dogs, 26 were seroreactive to an ehrlichia sp., 16 to babesia canis, and 25 to bartonella vinsonii, and 22 seroconverted to rickettsia rickettsii antigen ... | 1999 | 10405413 |
| ehrlichia platys infection and disease in dogs in spain. | | 1999 | 10424661 |
| fine structural characterisation of a rickettsia-like organism in human platelets from patients with symptoms of ehrlichiosis. | since 1982, ehrlichia platys infection has been diagnosed in canines from venezuela by the use of buffy coat smears. in 1992, ehrlichia-like bodies were observed in platelets from a severely ill girl by light microscopy. the patient was seropositive to e. chaffeensis by the indirect fluorescent antibody test (ifat). tetracycline was administered and the patient recovered. more than 400 cases with such intra-platelet organisms have been studied at this laboratory over the past 6 years, and all th ... | 1999 | 10535642 |
| detection of ehrlichia platys dna in brown dog ticks (rhipicephalus sanguineus) in okinawa island, japan. | thirty-two rhipicephalus sanguineus females collected from eight free-roaming dogs in okinawa island, japan, were examined for ehrlichial dna by 16s rrna-based pcr and subsequent sequencing. partial sequences of ehrlichia platys 16s rrna (678 to 679 bp) were detected in three ticks (9.4%) from two dogs. this is the first report of detection of e. platys in japan, and also the first report of detection in ticks. | 2000 | 11060094 |
| canine ehrlichiosis caused simultaneously by ehrlichia canis and ehrlichia platys. | to identify the causative agent of canine ehrlichiosis that has occurred in the suburbs of guangzhou, china, since 1998, the 16s rrna gene was amplified and sequenced. two sequences of 1,482 and 1,483 base pairs were obtained and named as gzh981 and gzh982, respectively. the level of similarity of these two was 91.50%, and gzh981 closely resembled the 16s rrna gene of ehrlichia canis, whereas gzh982 resembled ehrlichia platys. we therefore conclude that e. canis and e. platys together caused rec ... | 2000 | 11092236 |
| first detection of ehrlichia platys in dogs and ticks in okinawa, japan. | we investigated ehrlichia platys infection of dogs and ticks in okinawa, japan. using e. platys specific primers, e. platys and he3-r, pcr-positive results were obtained with 32.0% (64/200) of blood samples of dogs and 3.8% (3/77) of ticks. the nucleotide sequences of the amplified dna fragment from the dogs and the ticks infesting them were identical, and the sequence corresponded to that of the e. platys gzh981 strain. we concluded that there is a cyclic maintenance of e. platys between dogs a ... | 2001 | 11270612 |
| serologic and molecular evidence of coinfection with multiple vector-borne pathogens in dogs from thailand. | forty-nine dogs from thailand were evaluated for serologic evidence of exposure or polymerase chain reaction (pcr) evidence of infection with vectorborne pathogens, including ehrlichia sp. (ehrlichia canis, ehrlichia chaffeensis, ehrlichia equi, and ehrlichia risticii), bartonella vinsonii subsp. berkhoffi (bvb), spotted fever group (sfg) rickettsiae (rickettsia rickettsii), typhus group (tg) rickettsiae (rickettsia canada, rickettsia prowazekii, and rickettsia typhi), and babesia sp. (babesia c ... | 2001 | 11596732 |
| detection of ehrlichia platys in dogs in australia. | to describe the detection of ehrlichia platys in free-roaming dogs in central australia. | 2001 | 11599817 |
| differentiation of ehrlichia platys and e. equi infections in dogs by using 16s ribosomal dna-based pcr. | we have encountered a previously unrecognized specificity problem when using the small-subunit ribosomal dna (16s rdna)-based pcr primers recommended for use in the identification of ehrlichia equi in clinical samples. these pcr primers annealed to e. platys 16s rdna in blood samples containing high levels of e. platys organisms. therefore, we designed and tested new pcr primers for the identification of e. equi. | 2001 | 11724889 |
| reorganization of genera in the families rickettsiaceae and anaplasmataceae in the order rickettsiales: unification of some species of ehrlichia with anaplasma, cowdria with ehrlichia and ehrlichia with neorickettsia, descriptions of six new species combinations and designation of ehrlichia equi and 'hge agent' as subjective synonyms of ehrlichia phagocytophila. | the genera anaplasma, ehrlichia, cowdria, neorickettsia and wolbachia encompass a group of obligate intracellular bacteria that reside in vacuoles of eukaryotic cells and were previously placed in taxa based upon morphological, ecological, epidemiological and clinical characteristics. recent genetic analyses of 16s rrna genes, groesl and surface protein genes have indicated that the existing taxa designations are flawed. all 16s rrna gene and groesl sequences deposited in genbank prior to 2000 a ... | 2001 | 11760958 |
| survey of tick infestation and tick-borne ehrlichial infection of dogs in ishigaki island, japan. | twelve (54.5%) of 22 free-roaming dogs in ishigaki island had tick infestation identified as rhipicephalus sanguineus. there were 121 ticks recovered and consisted of 28 females, 58 males, 22 nymphs and 3 larvae. infection of dogs possibly with canine ehrlichial pathogens was examined by both indirect immunofluorescence assay and polymerase chain reaction (pcr). two dogs of the 13 examined were sero-positive for the human granulocytic ehrlichia agent, and one of two dogs was pcr positive for ehr ... | 2001 | 11767058 |
| determination of the nucleotide sequences of heat shock operon groesl and the citrate synthase gene (glta) of anaplasma (ehrlichia) platys for phylogenetic and diagnostic studies. | the 1,670-bp nucleotide sequence of the heat shock operon groesl and the 1,236-bp sequence of the citrate synthase gene (glta) of anaplasma (ehrlichia) platys were determined. the topology of the groel- and glta-based phylogenetic tree was similar to that derived from 16s rrna gene analyses with distances. both groesl- and glta-based pcrs specific to a. platys were also developed based upon the alignment data. | 2002 | 12204973 |
| demonstration of anaplasma (ehrlichia) platys inclusions in peripheral blood platelets of a dog in japan. | a free-roaming dog in okinawa island showed anaplasma (ehrlichia) platys-like inclusions within the platelets of peripheral blood samples. the inclusions were positive in indirect fluorescence test with anti-a. phagocytophila serum. the platelet count of the dog was 170,000 microl(-1). the sequence analysis of the 16s rrna, citrate synthase and heat shock protein genes of dna from the infected platelets confirmed that the inclusions were a. platys. this is the first detection of a. platys inclus ... | 2002 | 12446100 |
| ehrlichia platys (anaplasma platys) in dogs from maracaibo, venezuela: an ultrastructural study of experimental and natural infections. | since 1982 ehrlichia platys, now emended as anaplasma platys, has been diagnosed in dogs from maracaibo, venezuela, using buffy coat smears stained with dip quick. three dogs were inoculated with an a. platys strain. when parasitemia reached 60-97%, blood samples obtained from the inoculated dogs and from two naturally infected dogs were centrifuged to obtain platelet-rich plasma, which was mixed with 0.1% glutaraldehyde at 37 c for 10 minutes. platelet pellets were fixed in 3% glutaraldehyde fo ... | 2003 | 12637754 |
| natural and experimental canine infections with a greek strain of ehrlichia platys. | | 1991 | 12673539 |
| detection of ehrlichia spp., anaplasma spp., rickettsia spp., and other eubacteria in ticks from the thai-myanmar border and vietnam. | a total of 650 ticks, including 13 species from five genera, were collected from animals, from people, or by flagging of the vegetation at sites on the thai-myanmar border and in vietnam. they were tested by pcr to detect dna of bacteria of the order rickettsiales: three anaplasma spp. were detected in ticks collected in thailand, including (i) anaplasma sp. strain anda465, which was considered a genotype of anaplasma platys (formerly ehrlichia platys) and which was obtained from dermacentor aur ... | 2003 | 12682151 |
| analysis of 16s rrna gene sequences of ehrlichia canis, anaplasma platys, and wolbachia species from canine blood in japan. | in the present study, three canine blood samples from japan, that were suspected to be ehrlichia positive were examined. after sequencing the 16s rrna genes, each dog was found to be infected either with ehrlichia canis (kagoshima 1), anaplasma platys (okinawa 1), or wolbachia sp. (okinawa 2). phylogenic analysis was performed on these sequences. the nearly entire 16s rrna sequence of kagoshima 1 was found to be most similar to the sequences from oklahoma and venezuela e. canis strains (1 base p ... | 2003 | 12860708 |
| epidemiological survey of anaplasma platys and ehrlichia canis using ticks collected from dogs in japan. | detection of anaplasma platys and ehrlichia canis in ticks recovered from dogs in japan was attempted using a species-specific nested pcr based on the 16s rrna gene. a total of 1211 ticks recovered from 1211 dogs from all over japan were examined for a. platys and e. canis. four tick samples from fukushima, miyazaki and kagoshima prefectures recovered from four different dogs showed a positive reaction for a. platys. although the four dogs did not show any clinical signs and no blood examination ... | 2003 | 12944048 |
| isolation of an anaplasma sp. organism from white-tailed deer by tick cell culture. | we used tick cell culture to isolate a bacterium previously referred to as the "white-tailed deer (wtd) agent" from two captive fawns inoculated with blood from wild wtd (odocoileus virginianus). buffy coat cells were added to ise6 tick cell cultures and incubated at 34 degrees c, and 8 days later, anaplasma-like inclusions were demonstrated in giemsa-stained culture samples. the microbes became established and could be continuously passaged in tick cells. the identity of a culture isolate desig ... | 2003 | 12958265 |
| first evidence of anaplasma platys in rhipicephalus sanguineus (acari: ixodida) collected from dogs in africa. | a total of 27 ticks, comprising rhipicephalus sanguineus (latreille) (n = 21), haemaphysalis leachi (andouin) (n = 4) and haemaphysalis paraleachi (camicas, hoogstraal & el kammah) (n = 2) were recovered from two clinically healthy female dogs in the democratic republic of the congo. dna of anaplasma platys was detected in a female r. sanguineus, using primers derived from the 16s rrna gene, which amplify members of the family anaplasmataceae . anaplasma platys dna was also detected in the blood ... | 2003 | 14621316 |
| molecular detection of anaplasma platys in dogs using polymerase chain reaction and reverse line blot hybridization. | several polymerase chain reactions (pcrs) and a reverse line blot hybridization (rlb) method were used to identify anaplasma platys in dogs held in a kennel in italy. whereas pcr techniques confirmed the presence of a. platys, the rlb method not only correlated the results obtained by pcr but also ruled out the presence of other species such as ehrlichia canis or e. chaffeensis. there was no correlation between infection status and age or breed of the dogs. polymerase chain reaction performed on ... | 2003 | 14667015 |
| chronic canine ehrlichiosis (ehrlichia canis): a retrospective study of 19 natural cases. | nineteen dogs from greece with chronic ehrlichiosis were studied. the dogs exhibited bicytopenia or pancytopenia, bone marrow hypoplasia, seroreactivity to ehrlichia canis (e. canis) antigens, and had no history of drug or radiation exposure. anorexia, depression, severe bleeding tendencies, hypoalbuminemia, and increased serum alanine aminotransferase activity were also hallmarks of the disease. all these animals eventually died, irrespective of the treatment applied. some dogs were also serolo ... | 2004 | 15131097 |
| anaplasma platys: an improved pcr for its detection in dogs. | this study compares two pcr assays for the detection of anaplasma platys in dog blood using primers based on the a. platys 16s rrna gene. the first approach utilized a "standard" pcr protocol composed of a "single-step" direct amplification using an ehrlichia genus-specific primer set. the second assay being a "nested" pcr screen that first involved a universal bacterial primer set that amplified the majority of the 16s rrna gene, followed by the nested round of pcr using an a. platys-specific p ... | 2005 | 15713449 |
| prevalence of ehrlichia canis infection in thrombocytopenic dogs from rio de janeiro, brazil. | infection with ehrlichia canis causes a highly variable, multisystemic disease in dogs. nevertheless, many clinicians in rio de janeiro, brazil, use the presence of only thrombocytopenia to make a presumptive diagnosis of e canis infection. | 2005 | 15732017 |
| detection and identification of ehrlichia spp. in ticks collected in tunisia and morocco. | a broad-range 16s rrna gene pcr assay followed by partial sequencing of the 16s rrna gene was used for the detection of members of the family anaplasmataceae in ticks in north africa. a total of 418 questing ixodes ricinus ticks collected in tunisia and morocco, as well as 188 rhipicephalus ticks from dogs and 52 hyalomma ticks from bovines in tunisia, were included in this study. of 324 adult i. ricinus ticks, 16.3% were positive for ehrlichia spp., whereas only 3.4 and 2.8% of nymphs and larva ... | 2005 | 15750072 |
| molecular detection of anaplasma platys in lice collected from dogs in australia. | | 2005 | 15971830 |
| detection of medically important ehrlichia by quantitative multicolor taqman real-time polymerase chain reaction of the dsb gene. | ehrlichia species are the etiological agents of emerging and life-threatening tick-borne human zoonoses, in addition to causing serious and fatal infections in companion animals and livestock. we developed the first tricolor taqman real-time polymerase chain reaction assay capable of simultaneously detecting and discriminating medically important ehrlichiae in a single reaction. analytical sensitivity of 50 copies per reaction was attained with templates from ehrlichia chaffeensis, ehrlichia ewi ... | 2005 | 16237220 |
| genetic characterization of anaplasma (ehrlichia) platys in dogs in spain. | this paper reports the first genetic characterization of anaplasma (ehrlichia) platys in spain from a naturally infected dog. the dog presented clinical signs compatible with canine ehrlichiosis. after dna extraction and pcr amplification, 16s rrna gene and citrate synthase gene ( glta) of this agent were amplified. the genbank accession number for the nucleotide sequence of the 16s rrna gene of this strain is ay530806. the a. platys strains registered in france and japan showed the highest simi ... | 2006 | 16629989 |
| molecular characterization of anaplasma platys strains from dogs in sicily, italy. | the genetic diversity of anaplasma platys (rickettsiales: anaplasmataceae) strains is currently poorly defined. the present study was designed to characterize a. patys strains in dogs from palermo, sicily, italy, using a combination of pcr and sequence analysis of the 16s rdna, heat shock operon groesl and citrate synthase (glta) genes. | 2006 | 16872489 |
| tick-transmitted diseases in dogs: clinicopathological findings. | in this article we describe the main clinicopathological findings of some tick-transmitted diseases observed in italy, due to ehrlichia canis and babesia canis, and most rarely anaplasma phagocytophilum and anaplasma platys. canine monocytic ehrlichiosis (cme) is a multisystemic disorder that is characterized by various clinical signs. acutely-infected dogs show various clinical and haematological abnormalities including fever, lymphadenopathy, anorexia, lethargy, depression and thrombocytopenia ... | 2006 | 16881415 |
| tick-borne rickettsial pathogens in ticks and small mammals in korea. | in order to investigate the prevalence of tick-borne infectious agents among ticks, ticks comprising five species from two genera (hemaphysalis spp. and ixodes spp.) were screened using molecular techniques. ticks (3,135) were collected from small wild-caught mammals or by dragging/flagging in the republic of korea (rok) and were pooled into a total of 1,638 samples (1 to 27 ticks per pool). from the 1,638 tick samples, species-specific fragments of anaplasma phagocytophilum (1 sample), anaplasm ... | 2006 | 16957192 |
| detection of anaplasma platys and babesia canis vogeli and their impact on platelet numbers in free-roaming dogs associated with remote aboriginal communities in australia. | to detect anaplasma platys and babesia canis vogeli infection, using polymerase chain reaction (pcr)-based assays, in free-roaming dogs associated with eight aboriginal communities in remote areas of australia and to determine the impact of infection through the assessment of platelet numbers. | 2006 | 16958629 |
| rickettsial agents in egyptian ticks collected from domestic animals. | to assess the presence of rickettsial pathogens in ticks from egypt, we collected ticks from domestic and peridomestic animals between june 2002 and july 2003. dna extracts from 1019 ticks were tested, using pcr and sequencing, for anaplasma spp., bartonella spp., coxiella burnetii, ehrlichia spp., and rickettsia spp. ticks included: 29 argas persicus, 10 hyalomma anatolicum anatolicum, 55 hyalomma anatolicum excavatum, 174 hyalomma dromedarii, 2 hyalomma impeltatum, 3 hyalomma marginatum rufipe ... | 2006 | 17004028 |
| pcr detection of anaplasma platys in blood and tissue of dogs during acute phase of experimental infection. | four dogs were experimentally infected with anaplasma platys to determine changes in real-time taqman pcr detection in blood and tissue, microscopically detectable parasitemia, and platelet concentrations during the first 28 days of infection. buffy-coat blood cells were pcr positive for a. platys dna at 4 days after inoculation and remained positive in all dogs until day 14. marked thrombocytopenia and low parasitemia occurred in dogs during that initial period. during 17 and 28 days post-inocu ... | 2007 | 17034792 |
| epidemiological survey of ehrlichia canis and related species infection in dogs in eastern sudan. | the infection rates of ehrlichia canis and related species in dogs in eastern sudan were examined using molecular methods. among 78 dogs examined, 63 (80.8%), 19 (24.4%), and 26 (33.3%) were positive for e. canis, anaplasma platys, mycoplasma haemocanis, and "candidatus mycoplasma haemoparvum," respectively. among these, 30 dogs were single-positive: 25 for e. canis, 2 for a. platys, 1 for m. hemocanis, and 2 for "c. m. haemoparvum." the rest of the dogs (48.7%) were positive for two or more pat ... | 2006 | 17114754 |
| detection of rickettsia rickettsii and bartonella henselae in rhipicephalus sanguineus ticks from california. | sixty-two questing adult rhipicephalus sanguineus (latreille) ticks were collected by direct removal from blades of turfgrass and adjacent concrete walkways at a suburban home in riverside county, ca, and tested for the presence of rickettsia, bartonella, and ehrlichia dna. polymerase chain reaction (pcr) was used to amplify fragments of the 17-kda antigen gene and the rompa gene of the spotted fever group rickettsiae. one male tick contained r. rickettsii dna; its genotype differed from r. rick ... | 2007 | 17294935 |
| detection of ehrlichia canis by pcr in different tissues obtained during necropsy from dogs surveyed for naturally occurring canine monocytic ehrlichiosis. | a molecular study for the detection of ehrlichia canis was carried out on tissues obtained at necropsy from randomly selected dogs with the intention of investigating naturally-occurring canine ehrlichiosis. the tissues evaluated for the presence of e. canis included lymph nodes, spleen, liver, bone marrow, and blood. eight of the 18 dogs included were found to be positive for e. canis by polymerase chain reaction (pcr) and sequencing of the 16s rrna gene. two dogs were positive for anaplasma pl ... | 2008 | 17368942 |
| molecular and serological evidence for anaplasma platys and babesia sp. infection in a dog, imported in belgium, from southern spain. | this case report describes a dog suffering from a co-infection with babesia and anaplasma parasites. anaplasma platys was found to be responsible for the anaplasmosis by molecular biology techniques, while microscopical and serological evidence was found for a coexistent babesiosis, although this could not be confirmed by polymerase chain reaction. moreover, the possible risk of import of exotic pathogens is highlighted. | 2007 | 17523964 |
| molecular characterization of thai ehrlichia canis and anaplasma platys strains detected in dogs. | canine monocytic ehrlichiosis caused by ehrlichia canis is of veterinary importance worldwide. in thailand, there has been little information available on e. canis and its phylogeny. the objective of this study was to characterize and establish molecular structure and phylogeny of thai ehrlichia and anaplasma strains. genus-specific primers for ehrlichia and anaplasma were used to amplify the 16s rrna gene from naturally infected canine blood samples, and these amplicon sequences were compared w ... | 2008 | 17643354 |
| molecular evaluation of the incidence of ehrlichia canis, anaplasma platys and babesia spp. in dogs from ribeirão preto, brazil. | canine monocytic ehrlichiosis caused by ehrlichiacanis is endemic in many regions of brazil. since thrombocytopenia is a common finding in infected dogs, many clinicians tend to use it as an indication for antibiotic treatment. polymerase chain reaction (pcr) and nested pcr were used to study the presence of e. canis, anaplasma platys and babesia spp. in thrombocytopenic and non-thrombocytopenic dogs from ribeirão preto, brazil. despite the high prevalence of e. canis infection among thrombocyto ... | 2009 | 17920967 |
| prevalence of ehrlichia canis, anaplasma platys, babesia canis vogeli, hepatozoon canis, bartonella vinsonii berkhoffii, and rickettsia spp. in dogs from grenada. | to identify the tick-borne pathogens in dogs from grenada, we conducted a serologic survey for ehrlichia canis in 2004 (104 dogs) and a comprehensive serologic and molecular survey for a variety of tick-borne pathogens in 2006 (73 dogs). in 2004 and 2006, 44 and 32 dogs (42.3% and 43.8%) were seropositive for e. canis, respectively. in 2006, several tick-borne pathogens were identified by serology and pcr. dna of e. canis, anaplasma platys, babesia canis vogeli, hepatozoon canis, and bartonella ... | 2008 | 18160223 |
| anaplasma platys in dogs, chile. | we conducted a 16s rrna nested pcr for the genus ehrlichia and ehrlichia spp. with blood samples from 30 ill dogs in chile. phylogenetic analysis was performed by using groesl gene amplification. we identified anaplasma platys as 1 of the etiologic agents of canine ehrlichiosis. | 2007 | 18252119 |
| application of 10% imidacloprid/50% permethrin to prevent ehrlichia canis exposure in dogs under natural conditions. | canine monocytic ehrlichiosis (cme) caused by ehrlichia canis is the most known canine tick-borne disease (tbd) spread throughout the world. preventing tick bites is a priority to reduce the risk of tbds and it was the aim of the present study to evaluate the efficacy of a combination of imidacloprid 10% and permethrin 50% (imper) (advantix; bayer ag, germany) in a spot-on formulation to control cme under field conditions. on january-march 2005, 845 dogs from two kennels in southern italy (kenne ... | 2008 | 18359166 |