| safety of hepatitis-b vaccines containing intact 20 nm particles. | | 1979 | 85121 |
| expression in escherichia coli of chemically synthesized genes for human insulin. | synthetic genes for human insulin a and b chains were cloned separately in plasmid pbr322. the cloned synthetic genes were then fused to an escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. the insulin peptides were cleaved from beta-galactosidase, detected by radioimmunoassay, and purified. complete purification of the a chain and partial purification of the b chain were achieved. these products were mixed, reduced, and r ... | 1979 | 85300 |
| [separation of the biosynthesis products of a mutant strain of actinomyces chrysomallus var. carotenoides and the identification of actinomycin antibiotics]. | an orange antibiotically active substance isolated from the mycelium of a mutant strain of actinomyces chrysomallus var. carotenoides was identified as a mixture of actinomycins according to its light absorption spectra, circular dichroism spectra, ir spectra and chromatographic comparison with the standard samples. a scheme for successive extraction of the biologically active substances from the mycelium resulting in isolation of a fraction enriched with antibiotic substances and a fraction enr ... | 1979 | 85432 |
| the patterns and distribution of euchrysine-binding grains in nonstimulated human peripheral t and non-t lymphocytes. | | 1979 | 86386 |
| effects of antiprostatic agents on 5alpha-dihydrotestosterone binding to rat hypophyseal and hypothalamic cytosol macromolecules. | the binding of 5alpha-dihydrotestosterone to the hypophyseal and hypothalamic cytosol macromolecules prepared from castrated male rats was observed. the effects of antiprostatic agents on 5alpha-dihydrotestosterone binding to both hypophyseal and hypothalamic cytosol macromolecules was examined. cyproterone acetate and chlormadionone acetate showed the significant inhibiting effects on 5alpha-dihydrotestosterone binding to 7-8 s macromolecules of cytosol from both hypophysis and hypothalamus. sc ... | 1979 | 86441 |
| campylobacter urinary infection. | | 1979 | 86820 |
| interactions of lysophospholipids and mast cells. | | 1979 | 86956 |
| specificity of human lymphocytes primed against allogeneic cells in vitro. ii. discrimination and cross-reactivity in repeated priming. | when studying the specificity of human lymphocytes primed in vitro against hla-d determinants on allogeneic cells, it was found that specific restimulation of the primed cells 10 days after the first priming did not influence their discriminatory power compared to cells primed only once. likewise, priming to one hla-d determinant and repeated priming against another hla-d determinant did not change the discrimination for the first priming antigen. neither was there any increased relative reactiv ... | 1979 | 87030 |
| binding studies with antibodies having phosphorylcholine specificity and fragments derived from their homologous streptococcus pneumoniae type 27 capsular polysaccharide. | the capsular polysaccharide from streptococcus pneumoniae type 27 (s-27) has been fragmented into oligosaccharides by controlled degradations. binding of these derived small ligands with heterogeneous rabbit anti-s-27 antibody of selected phosphorylcholine affinity indicates that a major immunodeterminant of s-27 is the phosphorylcholine moiety, but that these anti-pc. antibodies are also capable of binding to the polysaccharide backbone from which the p.c. has been removed. | 1979 | 87460 |
| guinea pig transfer factor: restriction of macrophage migration inhibitory activity to extraordinary dilution confirmed with demonstration of diminished potency of stored preparations. | | 1979 | 88292 |
| automatic recognition of inter-ictal epileptic activity in prolonged eeg recordings. | a method of automatic recognition and quantification of inter-ictal epileptic activity in the human eeg had previously been developed and tested using short recordings from awake subjects. this paper describes the adaptation of the method for use during overnight recordings in free-moving unattended patients, in combination with the already existing seizure monitoring system. eeg s were recorded from scalp and sphenoidal electrodes, using cable telemetry and a pdp-12 computer. the spike and shar ... | 1979 | 88339 |
| detection of adenovirus by enzyme-linked immunosorbent assay. | a solid-phase direct enzyme-linked immunosorbent assay (elisa) was developed for the detection of adenovirus antigen in extracts of infected cells by using antihexon serum. results with simulated clinical specimens consisting of normal nasal wash specimens seeded with varying concentrations of adenovirus type 5 showed that antigen could be detected in extracts of hep-2 cell cultures inoculated with 10(2.5) 50% tissue culture infective doses (tcid50) and 10(1.5) tcid50 after 2 and 4 days of incub ... | 1979 | 88457 |
| characterization of functional fc-receptor material from human lymphoblastoid cell lines. ii. serologic and cellular analysis. | | 1979 | 88475 |
| [mrna of mouse plasmacytoma. reverse transcription and translation in cell-free systems]. | total poly(a)-mrna from polyribosomes of mopc 21 mouse myeloma were investigated. poly(a)-mrna was released by two successive chromatography on oligo (dt)-cellulose. a 14s fraction of total poly(a)-mrna was obtained and partially purified by sucrose gradient centrifigation followed by acrylamide gel electrophoresis. as estimated from the electrophoretic analysis, the 14s mrna has three components, one of which appears to be 18s rrna and two others--mrnas with molecular weight of 5.2.10(5) and 3. ... | 1979 | 88667 |
| prolonged survival of corneal allografts in rabbits treated with cyclosporin a. | | 1979 | 90771 |
| effects of 90 days exposure to catalytically treated automobile exhaust in rats. | | 1979 | 92404 |
| [effect of sources of carbon, nitrogen, and phosphorus on the synthesis of proteases from bacillus subtilis cultures]. | the paper gives data on the influence of different sources of carbon, nitrogen and phosphorus on the accumulation of biomass and synthesis of neutral protease by bacillus subtilis str. 103. the highest proteolytic activity was obtained on the medium containing maltose or hydrolyzed starch as a carbon source, monopotassium phosphate as a phosphorus source, and ammonium sulphate as a nitrogen source. the enzyme activity increased upon an addition of albumin, peptone or casein. a study of the amino ... | 1979 | 95825 |
| giant group a marker chromosome in three human lymphoblastoid cell strains from nasopharyngeal carcinoma. | | 1978 | 95929 |
| hematological data of the laboratory-maintained bonnet (macaca radiata) and the langur (presbytis entellus entellus) monkeys. | | 1978 | 95998 |
| ultrastructural localization of calcium around the membrane of the surface connected system in the human platelet. | the localization of calcium in the membrane system of human platelets was determined by ultrahistochemical methods equipped with an electron probe x-ray microanalyzer. after potassium oxalate-glutaraldehyde treatment large amounts of electron opaque precipitates were observed around the membrane of the surface connected system. electron probe x-ray microanalysis clearly defined that the precipitates were composed of calcium oxalate. the localization of calcium on the membrane of the surface conn ... | 1978 | 96043 |
| the relative biological effectiveness of pions in the acute response of human skin. | | 1977 | 96068 |
| experimental hammondia hammondi infection in monkeys. | | 1978 | 96246 |
| [adaptation of lipase and phospholipase a activities in pancreas and pancreatic juice in rats with diets rich in triglycerides and phospholipids]. | lipid rich diets containing about 20% of triglycerides or phospholipids given to rats during 2 months were observed to increase lipase and phospholipase a2 activities in pancrease and pancreatic juice. the phospholipase and lipase activities are higher, respectively, on the phospholipid and triglyceride diet. lower effects are observed after a 7-day administration of diet containing 40% of total lipid. | 1978 | 96963 |
| [the effects of testosterone and estradiol on the activity of lysosomal enzymes in rat liver and kidneys]. | in in vitro tests with lysosomes isolated from the liver and kidneys of castrated rats of both sexes the action of testosterone and beta-estradiol in concentrations of 3.76.10(-4)m on the activity of beta-glucosidase, beta-galactosidase and acid phosphatase was investigated. testosterone is shown to reduce the total and free activity of the membrane-bound enzymes and to increase the release from the matrix lysosomes. estradiol proved less active than is testosteron. the renal lysosomes in vitro ... | 1978 | 97095 |
| an improved electrophoretic method for identifying antibiotics with special reference to animal tissues and animal feeding stuffs. | | 1978 | 97257 |
| comparison of kinetic and end-point diffusion methods for quantitating human serum immunoglobulins. | proficiency testing results were used to compare the kinetic and end-point versions of the single radial immunodiffusion method for quantitating human serum immunoglobulins. statistical analysis of the results indicated that the results were not normally distributed but that the log normal distribution gives the best fit of any of the well-known frequency distributions. consequently, statistical analysis of immunoglobulin results must be log transformed before parametric statistical tests can be ... | 1978 | 97309 |
| contribution of omega-oxidation to fatty acid oxidation by liver of rat and monkey. | contributions of omega-oxidation to overall fatty acid oxidation in slices from livers of ketotic alloxan diabetic rats and of fasted monkeys are estimated. estimates are made from a comparison of the distribution of 14c in glucose formed by the slices from omega-14c-labeled compared to 2-14c-labeled fatty acids of even numbers of carbon atoms and from [1-14c]acetate compared to [2-14c]acetate. these estimates are based on the fact that 1) the dicarboxylic acid formed via omega-oxidation of a om ... | 1978 | 97354 |
| quinine-like drug attacks resistant malaria strains. | | 1978 | 97402 |
| induction of arylhydrocarbon hydroxylase and udp glucuronosyl transferase by pcb in hepatic cell cultures. | the purpose of the investigation was to derive a hepatic cell line to be used in induction studies of drug metabolizing enzymes. two pure cell lines were isolated from primary liver cell cultures, one with an epithelial-like appearance, the other with a fibroblast-like appearance. the specific activities of arylhydrocarbon hydroxylase (ahh) and udp glucuronosyl transferase (udpgt) were greater in hepatocyte cultures than in primary cultures or in fibroblast cultures. pcb enhanced the activity of ... | 1978 | 98133 |
| comparisons of the effects of fenoterol on pca and histamine skin test in rats. | | 1978 | 98295 |
| fatigue-induced damage in glutaraldehyde-preserved heart valve tissue. | glutaraldehyde-preserved porcine mitral leaflet tissue has been subjected to extended accelerated fatigue loading in ringer's solution containing 0.15% glutaraldehyde. five tissue test pieces were subjected to cyclic tensile stresses of 50 and 200 gm. per square millimeter and to 300 million to 800 million accumulated fatigue cycles. tissue disruption occurred in each of the fatigued test pieces. tensile loading, apart from reducing the acuteness of the collagen waveform and thereby decreasing t ... | 1978 | 98672 |
| transfer of 2,4,5,2',4',5'-hexachlorobiphenyl and 2,2-bis-(p-chlorophenyl),1,1,1-trichloroethane (p,p'-ddt) from maternal to newborn and suckling rats. | pregnant rats were given a small dose of 14c-2,4,5,2',4',5'-hexachlorobiphenyl (hcb) and 3h-ddt intraperitoneally. the transfer of hcb and ddt through the placenta and milk was then investigated. transfer through the placenta was 2.7 and 1.5% (respectively) of the initial doses; transfer through milk was 39.2 and 21.5%. hcb is obviously more transferable than ddt through the placenta and milk, the ratio of the amount of hcb transferred through milk to the amount transferred through the placenta ... | 1978 | 104695 |
| effects of chronic epileptic foci on control of pyramidal tract neurons in monkeys. | five macaca mulatta monkeys were operantly conditioned to control the firing patterns of single precentral pyramidal tract neurons. the accuracy with which the monkeys could control normal ptns from within the focus was significantly poorer than ptns from contralateral, homotopic cortex. in comparison to nonepileptic monkeys, there was no significant difference in the accuracy with which ptns from cortex contralateral to interictal foci were controlled. by contrast, comparison of the time necess ... | 1978 | 104868 |
| specificity and biological distribution of coenzyme m (2-mercaptoethanesulfonic acid). | the specificity of the growth requirement of methanobacterium ruminantium strain m1 for a new coenzyme, 2-mercaptoethanesulfonic acid (hs--com), was examined. a variety of derivatives, analogs, and potential biosynthetic precursors of coenzyme m were tested; only a restricted range of thioether, thioester, and thiocarbonate derivatives of the cofactor were found to replace the hs--com requirement. bromoethanesulfonic acid (brch2ch2so3-), a halogenated analog of hs--com, potently inhibited the gr ... | 1979 | 104960 |
| transfer of plasmid-mediated antibiotic resistance from streptococci to lactobacilli. | the transmissible plasmid pambeta1, which codes for erythromycin and lincomycin resistance, was conjugally transferred from a lancefield group f streptococcus to a strain of streptococcus avium. both organisms served as pambeta1 donors for three strains of lactobacillus casei. introduction of pambeta1 into one of the l. casei strains caused the organism to lose its native 6.7 x 10(6)-dalton plasmid. loss of the native plasmid produced no alterations in the organism's growth characteristics or fe ... | 1979 | 104973 |
| morphometric investigations on endocrine glands. v. changes in the testes of wistar rats after application of chlormadinone acetate and norethisterone acetate. | in growth inhibiting tests of rat testes the tubular diameter and, somewhat less sensitive, the nuclear volume of the leydig cell follow the weight changes. chlormadinone acetate shows antigonadotropic activity only at high doses and a dose-dependent antiandrogenic activity. the dose-weight curve under norethisterone acetate has a minimum at 4.2 mg/rat/14 days; higher doses seem to be accompanied by androgenic efficiency. | 1979 | 85556 |
| the primary structure of lactobacillus casei thymidylate synthetase. ii. the complete amino acid sequence of the active site peptide, cnbr 4. | the 102 amino acid residues of cnbr 4, the largest of 5 cyanogen bromide peptides from the lactobacillus casei thymidylate synthetase were completely sequenced by means of limited tryptic, tryptic, chymotryptic, and staphylococcal protease peptides. cnbr 4 contains both of the cysteines in an enzyme subunit, with the 5-fluorodeoxyuridylate-reactive cysteine at residue 198 and the other at residue 244. | 1979 | 105004 |
| specific labelling of replicating spp1 dna: analysis of viral dna synthesis and identification of phage dna-genes. | specific labelling of replicating bacteriophage spp1 dna can be achieved by infection at nonpermissive temperature of a b. subtilis strain carrying the initation mutation dnab ts134. under these conditions host dna synthesis is reduced by 90 to 95%. this technique was used to identify cistrons of spp1 involved in phage dna synthesis and to define intermediates in spp1 replication. | 1978 | 105242 |
| experimental nasopharyngitis and pneumonia caused by chlamydia trachomatis in infant baboons: histopathologic comparison with a case in a human infant. | three infant male baboons were inoculated with a strain of chlamydia trachomatis isolated from a human infant with pneumonitis. one baboon, inoculated by intratracheal, nasopharyngeal, and oropharyngeal seeding, had rales, radiographic evidence of pneumonia, persistent nasopharyngeal c. trachomatis infection, and a four-fold rise in titer of antibody. at sacrifice 24 days after inoculation, nasopharynx, trachea, airways, and lung yielded c. trachomatis, and epithelial inclusions were seen by lig ... | 1979 | 108341 |
| isolation of a new lipoxygenase metabolite of arachidonic acid, 8. 11, 12-trihydroxy-5,9,14-eicosatrienoic acid from human platelets. | washed human platelets incubated with 1-14c-arachidonic acid (1mm) produced a new metabolite which migrated on thin layer chromatography close to thromboxane b2, but which was identified by mass spectrometry as a trihydroxy fatty acid. the mass spectrum was consistent with the assigned structure, 8,11,12-trihydroxy-5,9,14-eicosatrienoic acid (thete). platelet thete synthesis from arachidonate was not inhibited by preincubation with asprin or indomethacin but was blocked by 5,8,11,14-eicosatetray ... | 1979 | 108769 |
| evidence against serotonin involvement in the tonic component of electrically induced convulsions and in carbamazepine anticonvulsant activity. | intraventricular injection of 5,7-dihydroxytryptamine, selective destruction of descending serotoninergic neurons by 5,6-dihydroxytryptamine or electrolytic and chemical lesions of the nucleus raphe dorsalis did not affect the electroconvulsive threshold in rats. no effect was observed after the systemic administration of drugs known to increase central serotonin transmission, such as quipazine, m-chlorophenylpiperazine, and moderate doses of d-fenfluramine, whereas p-chlorophenylalanine, an inh ... | 1979 | 120546 |
| on the life cycle of theileria velifera (uilenberg, 1964) in the gut and the haemolymph of the tick vector amblyomma variegatum (fabriciu, 1794). | the development of theileria velifera in the gut and the haemolymph of the tick vector amblyomma variegatum is described. between days three and eight after repletion various sexual stages are present in the gut of the nymphs. zygotes were detected in the gut on day 14, while undifferentiated kinetes first appeared on day 15. in the haemolymph, differentiated kinetes were observed from day 16 after repletion onwards. zygotes and kinetes were also found after the moult, in the adult tick, for a p ... | 1979 | 120627 |
| streptomycin sensitivity of ribosomes isolated from a streptomycin-producing streptomyces griseus. | the streptomycin sensitivity of ribosomes derived from a streptomycin-producing streptomyces griseus was examined in a polyuridylic acid directed 14c-phenylalanine incorporating system. in order to get reproducible results it is essential to use cell-free extracts which do not inactivate streptomycin. this condition can be fulfilled by the combination of washed ribosomes of the streptomycin-producing strain and the 110 000 g supernatant of the streptomycin-nonproducing variant of s. griseus, bec ... | 1979 | 120678 |
| ultrastructural aspects of cardiac lymphatic capillaries in experimental cardiac hypertrophy. | | 1975 | 124810 |
| trna gene clusters in escherichia coli dna. | | 1975 | 127650 |
| morphology, surface markers, and in vitro responses of a human leukemic t cell. | | 1975 | 127678 |
| interaction of inhibitors with muscle phosphofructokinase. | the interaction of several inhibitors with muscle phosphofructokinase has been studied by both equilibrium binding measurements and kinetic analysis. at low concentrations of citrate a maximum of 1 mol is bound per mol of enzyme protomer. tight binding requires mgatp and very weak binding is observed in the absence of either magnesium ion or atp. itp at low concentrations cannot replace atp. in the presence of mgatp and at ph 7.0, the dissociation constant for the enzyme-citrate complex is 20 mu ... | 1975 | 127795 |
| flaujeac trait. deficiency of human plasma kininogen. | flaujeac trait plasma resembled hageman trait or fletcher trait, in that the intrinsic coagulation pathway, plasma fibinolytic pathway, kinin-forming system, permeability factor of dilution (pf/dil) phenomenon were abnormal. the defect in each assay was reconstituted by afactor separable from hageman factor or fletcher factor. this substance was an alpha-globulin with an approximate mol wt of 170,000. flaujeac plasma did not release a kinin upon incubation with kallikrein and was deficient in to ... | 1975 | 127805 |
| the nature of the protein moieties of cartilage proteoglycans of pig and ox. | proteoglycans extracted with 4m-guanidinium chloride from pig laryngeal cartilage and bovine nasal septum were purified by density-gradient centrifugation in cscl under 'associative' followed by 'dissociative' conditions [hascall & sajdera (1969) j. biol. chem. 244, 2384-2396]. proteoglycans were then digested exhaustively with testicular hyaluronidase, which removed about 80% of the chondroitin sulphate. the hyaluronidase was purified until no proteolytic activity was detectable under the condi ... | 1975 | 128355 |
| inhibitory effects of mersalyl and of antibodies directed against smooth-muscle myosin on a calcium adenosine triphosphatase of the plasma membrane from mouse liver cells. | | 1976 | 130830 |
| anatomically orthogonal qrs electrocardiogram of the baboon (papio hamadryas) and the macaque (macaca mulatta). sex and species differences. | peak amplitudes of the qrs complex were evaluated in 28 adult macaques (macaca mulatta) and 28 adult baboons (papio hamadryas), using two anatomically orthogonal lead systems. the anteroposterior (z) and the transversal (x) component of the cardiac electric field was smaller in females than in males. comparison of qrs amplitudes in baboons and macaques indicates that the loop of the spatial vectorcardiogram is situated further ventrally and to the right in the former. the relationship between ve ... | 1977 | 140402 |
| [immunocytological study of the gonadotrophic and thyrotrophic cells of the rat adenohypophysis]. | on paraffin or semi-thin sections various anti-lh or anti-tsh sera stain indifferently all the thyrotrophs and the gonadotrophs. inversely anti-beta-tsh, anti-beta-lh or anti-beta-fsh purified sera permit the discrimination of these two cell populations. the constancy of fixation of the anti-beta-lh and anti-beta-fsh sera on all the gonadotrophs gives evidence of their ability to produce both fsh and lh. however in a few female rats the central gonadotrophs are stained more weakly by anti-beta-f ... | 1977 | 145301 |
| bronchial spasmolytic action of terbutaline and fenoterol compared with that of ipratropium bromide in the anaesthetized dog. | continuous recordings of heart rate, respiration rate, minute volume and bronchial resistance were made over a period of altogether three hours in spontaneously breathing dogs anaesthetized with chloralose. at hourly intervals, before and 1 and 2 hr after the administration of bronchodilators, bronchial spasm was induced by inhalation of an acetylcholine solution. two beta2-stimulants, terbutaline and fenoterol, were administered by inhalation in doses of 0.5, 0.16 and 0.05 mg/kg and 0.1, 0.3 an ... | 1978 | 152096 |
| [morphological changes in the digestive organs during prolonged space flight on the kosmos-782 biosatellite]. | a reduction in the content of neutral mucopolysaccharides in mucous cells of the neck, a slight decrease in the activity of succinate dehydrogenase and nad-diaphorase in parietal cells, a decrease in the dna synthesis rate, and an increase in the area of mitochondria and cristae were detected in the gastric mucosa of rats which were in a long-term space flight. in the small intestine, an increase in the activity of glucose-6-phosphate dehydrogenase and leucine aminopeptidase were found. morpholo ... | 1978 | 152100 |
| [intravenous cholangiography in hyperbilirubinaemia (author's transl)]. | the effect of bilirubin on biliary and renal excretion of methylglucamine-iodipamide at an infusion rate of 1.3, 2.6 and 5.2 mu mol/min/kg was studied by intravenous injection into five dogs with normal liver functions and chronic duodenal fistulas. unconjugated bilirubin administered in a dose of 0.1 mu mol/min/kg reduced biliary iodipamide excretion and concentration significantly, but did not affect renal contrast material excretion. this indicates that the unsatisficatory radiological result ... | 1979 | 153309 |
| effect of corn and sorghum diets in n1 methylnicotinamide excretion and hepatic enzymes in rats. | this study was carried out to compare the effects of corn (zea mays) and sorghum (sorghum vulgare) diets on urinary n1 methylnicotinamide (n'mn) excretion and on the activity of hepatic enzyme in young adult rats. thirty rats, weighing an average of 174.3 g at the beginning of the experiments, were divided into three groups and studied for 13 weeks. the two experimental diets supplied 7% protein, and a casein diet was used as control. niacin was excluded from the vitamin mixture used in the corn ... | 1978 | 153892 |
| immunobiologic factors in the pathogenesis of renal infection. | | 1979 | 161989 |
| avian rna tumor viruses. a model for studying tumor associated cell surface alterations. | | 1975 | 164248 |
| immunologic aspects of 1, 2-dimethylhydrazine-induced colon tumors in rats. | investigation of immunologic aspects of colon tumors induced in rats by 1,2-dimethylhydrazine demonstrated that: 1) an antigen in an extract of colon tumors was not detected in the normal colon. it was related to antigens in rat fetuses and did not cross-react immunologically with carcinoembryonic antigen. 2) mucinous adenocarcinomas of the colon were significantly associated spatially with lymphoid follicles. | 1975 | 165314 |
| sex specific oestrogen binding in the middle hypothalamus of rats. | | 1975 | 179802 |
| transport of retinol from the blood to the retina: involvement of high molecular weight lipoproteins as intracellular carriers. | | 1976 | 179832 |
| malignant transformation-linked imbalance: decreased xanthine oxidase activity in hepatomas. | xanthine oxidase was decreased 2- to 10-fold in all examined rat hepatomas irrespective of the malignancy; growth rate and degrees of histological differentiation of the neoplasms. the affinity to substrate (km=6-8 mum) and the ph optimum (8.0) of the liver and hepatoma enzymes were the same. the reprogramming of gene expression, as manifested in the decreased activity of this key purine metabolizing enzyme, appears to be specific to neoplastic transformation. since glutamine prpp amidotransfera ... | 1975 | 179860 |
| isolation and localization of rna fractions able to transfer tumor-specific delayed hypersensitivity in vitro. | rna fractions were prepared by sucrose density gradient centrifugation from hot-cold phenol, rna-rich extracts of lymphoid tissues from strain 2 guinea pigs hyperimmunized to line 1 or line 10 tumors. each rna fraction was assessed for its ability to convert nonsensitized strain 2 peritoneal exudate cells to a state of specific sensitivity for line 1- or line 10-solubilized tumor antigens. an rna fraction residing between the 4 s and 18 s peaks, designated as fraction "b", transferred line 10 or ... | 1976 | 187327 |
| structural studies and physical maps for the herpes simplex virus genome. | | 1977 | 193820 |
| 3(17)beta-hydroxysteroid dehydrogenase of pseudomonas testosteroni. a convenient purification and demonstration of multiple molecular forms. | | 1977 | 193845 |
| [study of cytoplasmic rna in tissues of the rat, regenerating liver and in liver of rats with hepatoma pc-1]. | cytoplasmic rna of the rat regenerating liver and liver of rats with hepatoma pc-1 is separated into five fractions on the column of kieselgur with methylated albumin (mak). studies in the nucleotide composition showed that certain fractions of cytoplasm rna are ribosomal and messenger rna. the highest amount of radioactive precursor is incorporated into messenger rna of the rat regenerating liver and liver of the rats with hepatoma pc-1 which is eluated from the column by 0.2% solution of sodiu ... | 1977 | 201068 |
| the presence of disulfide-linked gp70-p15(e) complexes in akr murine leukemia virus. | | 1977 | 201090 |
| delayed-type skin hypersensitivity reaction (dth) to thomsen-friedenreich (t) antigen as diagnostic test for human breast adenocarcinoma. | one intradermal (i.d.) injection of human erythrocyte t antigen in the upper outer arm contralateral to any breast lesion elicits a delayed tuberculin-type hypersensitivity reaction (dth) in breast carcinoma patients. it is necessary to inject simultaneously but separately the same quantity of mn antigen (about 6--8 cm apart), from which the t antigen has been prepared, since particularly patients with stage i breast carcinoma (internatl. nomenclature) and those with benign breast disease may si ... | 1979 | 228112 |
| failure of indicator bacteria to reflect the occurrence of enteroviruses in marine waters. | the results of several studies conducted along the upper texas gulf coast, where a substantial amount of quantitative virological data were collected, are compared to bacteriological indicators and other environmental factors on a statistical basis. variables common to all these studies were anlayzed by multivariate regression. although multivariate analysis indicated that the number of viruses detected in water was related to rainfall, salinity, and total coliforms in the water, the amount of v ... | 1979 | 228561 |
| biochemical studies with infectious bursal disease virus: comparison of some of its properties with infectious pancreatic necrosis virus. | infectious bursal disease (ibd) virus was purified from the bursae of infected chickens. two morphologically indistinguishable populations of virus particles were separated in sucrose gradients and possessed sedimentation coefficients of 295s and 460s. both populations contained rna and had identical polypeptide compositions. ibd virus banded at a density of 1.31 g/ml in cscl and at 1.24 g/ml in sodium potassium tartrate. ibd virus contained two rna segments with mol. wts. of 2.4x10(6) and 2.2x1 ... | 1979 | 228637 |
| marek's disease lymphoma of chicken and its causative herpesvirus. | | 1979 | 228727 |
| search activity and its impact on experimental and clinical pathology. | | 1979 | 229689 |
| mode of initiation of cell infection with sludge-associated poliovirus. | association with sludge solids did not allow poliovirus to enter cells other than through the normal receptor-mediated route. this implies that the infectivities of sludge-encapsulated virions are masked until their exposure permits binding to cell surface receptors. | 1979 | 229768 |
| lead-cadmium interaction: effects on viral-induced mortality and tissue residues in mice. | simultaneous exposure to lead and cadmium is highly probable due to common environmental sources of exposure. mice were exposed simultaneously for 10 wek to lead and cadmium and effects were observed on accumulation of the metals in tissues and susceptibility to viral challenge. the incidence of viral-induced mortality was enhanced in all groups of mice exposed to lead and suppressed in groups of mice treated with cadmium. mice exposed to both lead and cadmium had mortality rates intermediate to ... | 1979 | 229776 |
| effect of dithiocarb and dimethyl sulfoxide on irreversible binding of 14c-bromobenzene to rat liver microsomal protein. | the irreversible binding of [14c]-bromobenzene to rat liver microsomal protein in vitro was inhibited by dithiocarb and dmso. dithiocarb suppressed this binding in a time- and concentration-dependent manner (i50 = 4.5 10(-5) m). dmso reduced the degree of covalent binding by 61% from 5 x 10(-5) m to 8 x 10(-4) m. dithiocarb was also effective in inhibiting irreversible binding of bromobenzene to liver protein in vivo. our results are consistent with the hypothesis that dithiocarb exerts its anti ... | 1979 | 229786 |
| stereochemical courses of nucleotidyltransferase and phosphotransferase action. uridine diphosphate glucose pyrophosphorylase, galactose-1-phosphate uridylyltransferase, adenylate kinase, and nucleoside diphosphate kinase. | | 1979 | 229894 |
| stability of the "two active x" phenotype in triploid somatic cells. | we examined triploid cells of xxy karyotype heterozygous for glucose 6 phosphate dehydrogenase (g6pd) electrophoretic variants with regard to the stability of their x chromosome phenotype. clonal populations of cells derived from these human fibroblasts maintained a precise 1:2:1 ratio of a:heteropolymer:b isozymes throughout their life span, indicating stability of the two active x chromosomes in these cells. to determine the influence of the autosomal complement on x chromosome expression, we ... | 1979 | 229959 |
| [md/vd infection as a cause of bovine abortion (brief clinical report)]. | | 1979 | 230019 |
| the influence of artobotrys odoratissimus linn. extracts on phosphatase activity in the uterus of rats. | the effect of 50% ethanolic and benzene extracts of artobotrys odoratissimus linn., a potent antiestrogenic plant on uterine acid and alkaline phosphatase activity has been studied in adult rats. both the extracts decreased the alkaline phosphatase activity in the uterus of intact and ovariectomized rats (p less than 0.001) but elevated the acid phosphatase activity (p less than 0.001). 50% ethanolic extract acts at low dose levels; however its activity decreases as the dose is increased. benzen ... | 1979 | 230155 |
| diffusion-controlled reactions on spherical surfaces. application to bacteriophage tail fiber attachment. | we have explored the kinetic implications of a model that may account for the acceleration of tail fiber (f) attachment to baseplates (b) by whiskers (w) on bacteriophage t4. the model assumes that a w-f complex is formed initially, and that the tethered fiber then undergoes rotational diffusion until a b-f encounter takes place. in the absence of whiskers, b-f complexes must form unassisted. formation of a w-f intermediate will accelerate f attachment to b if (a) the bimolecular rate constant f ... | 1979 | 262439 |
| influence of the substituent radicals in antimonyl naphthalein derivatives on the potentialities of anti-schistosomal activity in mice. | | 1979 | 262474 |
| an evaluation of three new commercial peptones used in some nutrient media designed for the isolation and characterization of pathogenic vibrios. | | 1979 | 262509 |
| comparative morphologic and histochemical study on the habenular nuclei in some species of mammals of the family muridae. | | 1978 | 308917 |
| cytotoxic activities of normal cultured human t cells. | normal human t cells grown in continued cultures in medium containing conditioned medium (cm) from pha-stimulated lymphocytes were studied for their ability to manifest three known forms of cell-mediated cytotoxicity: lectin-induced cellular cytotoxicity (licc), natural killer cell (nk) activity, and antibody-dependent cellular cytotoxicity (adcc). the cultured t cells (ctc) were very effective mediators of licc, being cytotoxic even at very low attacker-target cell ratios in the presence of dif ... | 1978 | 308961 |
| influenza virus infection in newborn rats: a possible marker of attenuation for man. | the growth of parent influenza viruses a/england/939/69 and a/pr/8/34, and clones 6, 7, and 64c, derived by recombination, was studied in newborn rats. using an inoculum of 10(4.0) eid50, influenza virus a/england/939/69 produced the highest titres of virus in rat turbinates at 48 hours after inoculation; clones 6 and 7 and a/pr/8/34 grew to lower titres; and clone 64c grew to the lowest titre. these differences were less apparent when 10(2.0) eid50 of virus was used as an inoculum, and rats wer ... | 1978 | 308996 |
| absence of increasing incidence of meningitis caused by haemophilus influenza type b. | an epidemiologic survey of meningitis caused by haemophilus influenzae type b in children aged zero to four years during an 11-year period (january 1965-december 1975) was conducted in the baltimore, maryland, metropolitan area to examine recent trends in the incidence of this disease. cases of h. influenzae meningitis were identified at all 19 hospitals in the city and county of baltimore and all 41 hospitals in the surrounding area. the population at risk (age, zero to four years) was estimate ... | 1979 | 317292 |
| immune response of ponies to experimental infection with ehrlichia equi. | four ponies experimentally infected with ehrlichia equi developed substantial cell-mediated immune responses, as measured by the leukocyte migration-inhibition test. serum anti-e equi antibodies up to 1:1,280 were demonstrated by the indirect fluorescent antibody test. cell-mediated immune responses returned to a base-line value by day 200 after primary inoculation, but serum antibody titers persisted for at least 300 days after inoculation. two additional susceptible ponies, which were inoculat ... | 1978 | 343663 |
| intestinal apoproteins during fat absorption. | to compare the roles of apolipoprotein (apo) a-i, b, and e (or arginine-rich apoprotein, arp) in the intracellular production of intestinal chylomicrons (and/or vldl), these apoproteins were localized in rat intestinal mucosa by the light microscope method of indirect immunofluorescence. in addition, tissue levels of apoa-i and apob were measured during fat absorption by radioimmunoassay. antisera were produced using apoa-i isolated from rat plasma high density lipoprotein, and apob and arp from ... | 1978 | 350901 |
| alteration in local and systemic immune capacity after exposure to bursts of co. | | 1979 | 387409 |
| quantitative estimation of leishmanial antibody titers by enzyme-linked immunosorbent assay. | the response to immunization against leishmania braziliensis in rabbits was followed by measurement of antibody by enzyme-linked immunosorbent assay (elisa), passive hemagglutination, complement fixation, and countercurrent immunoelectrophoresis. passive hemagglutination and complement-fixation titers were interpreted according to standard methods, whereas elisa exact titers were derived by the equation y = a-b log x, which describes the straight line that results when absorbances are plotted ag ... | 1979 | 387886 |
| histopathology of retinal lesions produced by long-term laser exposure. | the maculae of rhesus monkeys were exposed for 120/s to nd-yag and argon laser radiation at wavelengths of 1060 and 514.5 nm, respectively. histopathology of lesions associated with a thermal and a nonthermal damage mechanism were compared. differences were observed for both lesion development and retinal layers affected, depending upon the damage mechanism involved. | 1977 | 407896 |
| the isolation by immunoabsorbent affinity chromatography and physicochemical characterization of mycobacterium tuberculosis antigen 5. | mycobacterium tuberculosis antigen 5 was purified from unheated culture filtrates by absorption onto an immunoabsorbent prepared with globulin from a monospecific goat antiserum and elution with 4.0 m urea at ph 9.0. the product was a homogeneous protein giving a single stainable band in acrylamide gel electrophoresis and a single precipitin arc in immunoelectrophoresis. it was found to have a molecular weight of 28,500 to 35,000 daltons and a sedimentation constant of 2.0. amino acid analysis d ... | 1978 | 415649 |
| effect of age, weight and adequacy of zinc intake on the balance between alkaline ribonuclease and ribonuclease inhibitor in various tissues of the rat. | 1. deficiency of zinc inhibits growth and also increases the activity of alkaline ribonuclease in certain tissues of the rat (prasad & oberleas, 1973). zn could influence ribonuclease activity by direct effects on the enzyme or its natural inhibitor, or non-specifically as occurs when growth rate is affected by various other factors. these possibilities were studied. 2. alkaline ribonuclease was shown to be inhibited by zn in vitro, but the concentrations of zn required were so high that the enz ... | 1978 | 415757 |
| effects of ccnu on hematopoiesis in rats. | the effect of orally administered ccnu on proliferating and cytokinetically "resting" bone marrow cells was studied in rats. the resting bone marrow cells were selectively labeled with 3h-thymidine by the "complete 3h-thymidine labeling method." the results show a reduction in the number of absolute bone marrow cells to about 40% of the original value in 3 days. regeneration was completed only after 22 days. no difference was found in the destructive or the regenerative pattern of cfu-c, myelopo ... | 1979 | 427820 |
| [dynamics of the development of different microorganisms in soil]. | the dynamics of individual components from the complex of soil microorganisms (bacteria, fungi, actinomycetes) and the population of stm. olivocinereus introduced into soil was studied by luminescent microscopy and inoculation. the population density maxima for individual components from the complex of soil microorganisms were shown to be separated in time suggesting a succession. fungi developed at the first steps of succession and dominated in biomass over the remaining components of the compl ... | 1979 | 470632 |
| free and esterified cholesterol concentration and cholesteryl ester composition in the ovaries of maturing and superovulated immature rats. | the cholesterol and cholesteryl ester concentration and cholesteryl ester composition were determined in the ovaries of immature rats, sexually mature rats and superovulated immature rats. the immature rat ovary accumulated cholesteryl esters, and long-chain polyunsaturated fatty acids were preferentially incorporated into these esters. the cholesteryl esters decreased in concentration and changed in composition with the onset of the first estrous cycle. superovulation of immature rats, by injec ... | 1979 | 508780 |
| voltage-clamp studies on the canine purkinje fibre [proceedings]. | | 1979 | 529143 |
| [the so-called "rejuvenating agents" from the viewpoint of experimental gerontology]. | | 1979 | 538944 |
| a sensitive fluorimetric microassay for the determination of glutathione peroxidase activity. application to human blood platelets. | | 1979 | 543550 |
| [existence of glucocorticoid-specific receptors in the plasma membranes of rat liver]. | it was found that isolated plasma membranes whose purity was assayed by determinations of marker enzyme activities, specifically bind dexamethasone. the association constant and the number of binding sites were found to be equal to (7,03 +/- 4,05) . 10(9) m-1 and (1,6 +/- 0,18) . 10(-14) mol/mg protein, respectively. it was assumed that lipoprotein components of plasma membranes are involved in this binding. | 1979 | 546445 |