| kinetics of antibody response to ehrlichia canis assayed by the indirect fluorescent antibody method. | the kinetics of antibody production response to experimentally induced infection of dogs with ehrlichia canis was determined by ion-exchange and molecularsieve chromatography and by indirect fluorescent antibody (ifa) test. the first ifa antibody at 7 days after inoculation resided in immunoglobulin m (igm) and immunoglobulin a (iga) classes. at approximately 21 days after inoculation, the antibody was in igm, iga, and immunoglobulin g (igg) classes. thereafter, antibody concentrations continued ... | 1975 | 49162 |
| effect of quantity and quality of igg antibodies on blocking of allergenic histamine release in vitro. | in a study of the protective role of igg antibodies in allergen injection therapy, serum antibodies to timothy grass allergens were raised in rabbits and tested for inhibition of histamine release from basophils of grass pollen-sensitive human subjects. rabbit sera were not inhibitory until late in the immune response when large amounts of relatively low affinity igg antibodies were detected by primary binding. inhibition of histamine release by late sera required preincubation of allergen and a ... | 1979 | 84798 |
| fatal adenovirus infection with misleading positive serology for infectious mononucleosis. | symptoms and serological tests in a 19-year-old man with pneumonia indicated that he had infectious mononucleosis. adenovirus type 7 was demonstrated in the lung by electron microscopy and was cultured from the liver, lung, lymph-nodes, and kidney. | 1979 | 84950 |
| safety of hepatitis-b vaccines containing intact 20 nm particles. | | 1979 | 85121 |
| expression in escherichia coli of chemically synthesized genes for human insulin. | synthetic genes for human insulin a and b chains were cloned separately in plasmid pbr322. the cloned synthetic genes were then fused to an escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. the insulin peptides were cleaved from beta-galactosidase, detected by radioimmunoassay, and purified. complete purification of the a chain and partial purification of the b chain were achieved. these products were mixed, reduced, and r ... | 1979 | 85300 |
| [mechanisms of the potentiating and blocking effects of the sera from tumor patients in the leukocyte migration inhibition reaction model]. | in the model of the reaction of human blood leucocyte migration inhibition the in vitro study was conducted on some mechanisms of blocking and enhancement effects of the serum in 54 tumor patients. both effects were shown to be specific to an antigen. the enhancement effect could render a nonspecific action on intact cells in the presence of the corresponding antigen. this effect is thermolabile and may occur due to the interaction with cells. the blocking effect is thermostabile and may be real ... | 1979 | 85367 |
| the patterns and distribution of euchrysine-binding grains in nonstimulated human peripheral t and non-t lymphocytes. | | 1979 | 86386 |
| effects of antiprostatic agents on 5alpha-dihydrotestosterone binding to rat hypophyseal and hypothalamic cytosol macromolecules. | the binding of 5alpha-dihydrotestosterone to the hypophyseal and hypothalamic cytosol macromolecules prepared from castrated male rats was observed. the effects of antiprostatic agents on 5alpha-dihydrotestosterone binding to both hypophyseal and hypothalamic cytosol macromolecules was examined. cyproterone acetate and chlormadionone acetate showed the significant inhibiting effects on 5alpha-dihydrotestosterone binding to 7-8 s macromolecules of cytosol from both hypophysis and hypothalamus. sc ... | 1979 | 86441 |
| treatment of mammalian ova for the demonstration of nucleoprotein structures. | | 1979 | 86485 |
| specificity of human lymphocytes primed against allogeneic cells in vitro. ii. discrimination and cross-reactivity in repeated priming. | when studying the specificity of human lymphocytes primed in vitro against hla-d determinants on allogeneic cells, it was found that specific restimulation of the primed cells 10 days after the first priming did not influence their discriminatory power compared to cells primed only once. likewise, priming to one hla-d determinant and repeated priming against another hla-d determinant did not change the discrimination for the first priming antigen. neither was there any increased relative reactiv ... | 1979 | 87030 |
| binding studies with antibodies having phosphorylcholine specificity and fragments derived from their homologous streptococcus pneumoniae type 27 capsular polysaccharide. | the capsular polysaccharide from streptococcus pneumoniae type 27 (s-27) has been fragmented into oligosaccharides by controlled degradations. binding of these derived small ligands with heterogeneous rabbit anti-s-27 antibody of selected phosphorylcholine affinity indicates that a major immunodeterminant of s-27 is the phosphorylcholine moiety, but that these anti-pc. antibodies are also capable of binding to the polysaccharide backbone from which the p.c. has been removed. | 1979 | 87460 |
| guinea pig transfer factor: restriction of macrophage migration inhibitory activity to extraordinary dilution confirmed with demonstration of diminished potency of stored preparations. | | 1979 | 88292 |
| detection of adenovirus by enzyme-linked immunosorbent assay. | a solid-phase direct enzyme-linked immunosorbent assay (elisa) was developed for the detection of adenovirus antigen in extracts of infected cells by using antihexon serum. results with simulated clinical specimens consisting of normal nasal wash specimens seeded with varying concentrations of adenovirus type 5 showed that antigen could be detected in extracts of hep-2 cell cultures inoculated with 10(2.5) 50% tissue culture infective doses (tcid50) and 10(1.5) tcid50 after 2 and 4 days of incub ... | 1979 | 88457 |
| characterization of functional fc-receptor material from human lymphoblastoid cell lines. ii. serologic and cellular analysis. | | 1979 | 88475 |
| studies on mononuclear phagocyte progenitor cells: morphology of cells and colonies in liquid culture of mouse bone marrow. | | 1979 | 88518 |
| a technique for investigating the antigenicity of cultured rheumatoid synovial cells. | antisera to rheumatoid and nonrheumatoid synovial cells were prepared by: intraarticular injection of rabbits with these cells, subsequent synovectomy, and autologous immunization of each animal with the cultured rabbit synovial cells, or homogenized whole rabbit synovial membrane. resultant antisera were studied by immunofluorescence and 51chromium cytotoxicity tests against other cultured rheumatoid and nonrheumatoid cells. activity of positive sera could be removed by absorption with nonrheum ... | 1979 | 88519 |
| use of antibodies for studying the sidedness of membrane components. | | 1979 | 88659 |
| [mrna of mouse plasmacytoma. reverse transcription and translation in cell-free systems]. | total poly(a)-mrna from polyribosomes of mopc 21 mouse myeloma were investigated. poly(a)-mrna was released by two successive chromatography on oligo (dt)-cellulose. a 14s fraction of total poly(a)-mrna was obtained and partially purified by sucrose gradient centrifigation followed by acrylamide gel electrophoresis. as estimated from the electrophoretic analysis, the 14s mrna has three components, one of which appears to be 18s rrna and two others--mrnas with molecular weight of 5.2.10(5) and 3. ... | 1979 | 88667 |
| the influenza virus: antigenic composition and immune response. | the architecture and chemical composition of the influenza virus particle is described with particular reference to the protein constituents and their genetic control. the dominant role in infection of the surface proteins - haemagglutinins and neuraminidases - acting as antigens and undergoing variation in time known as antigenic drift and shift is explained. the immuno-diffusion technique has illuminated the interrelationships of the haemagglutinins of influenza a viruses recovered over long p ... | 1979 | 88726 |
| inhibition of spontaneous transformation of rat embryo cells releasing endogenous type c virus by virus-specific antiserum. | noninbred sprague-dawley rat embryo cell clones predictably undergo transformation after 20-30 in vitro passages following spontaneous release of endogenous rat leukemia virus (ralv). in the presence of ralv-specific antiserum, virus production and infectivity were reduced and transformation was delayed from 6 to 25 weeks. transformation was not associated with an increased expression of kirsten murine sarcoma virus-related src gene rna. | 1979 | 89211 |
| amylase levels in the tissues and body fluids of the domestic cat (felis catus). | 1. the amylase activities of serum, liver, pancreas, submaxillary and parotid glands, saliva, duodenum, colon, lung, heart, spleen, kidney and skeletal muscle of the domestic cat, were determined. 2. as in most mammals, the highest amylase content was found in the pancreas. levels in saliva and salivary glands were very low. 3. amylase activities of the serum, liver and pancreas have an optimum ph 7.0-7.5. 4. amylases of serum, liver and pancreas were inactivated by removal of chloride and these ... | 1979 | 95689 |
| giant group a marker chromosome in three human lymphoblastoid cell strains from nasopharyngeal carcinoma. | | 1978 | 95929 |
| hematological data of the laboratory-maintained bonnet (macaca radiata) and the langur (presbytis entellus entellus) monkeys. | | 1978 | 95998 |
| ultrastructural localization of calcium around the membrane of the surface connected system in the human platelet. | the localization of calcium in the membrane system of human platelets was determined by ultrahistochemical methods equipped with an electron probe x-ray microanalyzer. after potassium oxalate-glutaraldehyde treatment large amounts of electron opaque precipitates were observed around the membrane of the surface connected system. electron probe x-ray microanalysis clearly defined that the precipitates were composed of calcium oxalate. the localization of calcium on the membrane of the surface conn ... | 1978 | 96043 |
| [adaptation of lipase and phospholipase a activities in pancreas and pancreatic juice in rats with diets rich in triglycerides and phospholipids]. | lipid rich diets containing about 20% of triglycerides or phospholipids given to rats during 2 months were observed to increase lipase and phospholipase a2 activities in pancrease and pancreatic juice. the phospholipase and lipase activities are higher, respectively, on the phospholipid and triglyceride diet. lower effects are observed after a 7-day administration of diet containing 40% of total lipid. | 1978 | 96963 |
| [the effects of testosterone and estradiol on the activity of lysosomal enzymes in rat liver and kidneys]. | in in vitro tests with lysosomes isolated from the liver and kidneys of castrated rats of both sexes the action of testosterone and beta-estradiol in concentrations of 3.76.10(-4)m on the activity of beta-glucosidase, beta-galactosidase and acid phosphatase was investigated. testosterone is shown to reduce the total and free activity of the membrane-bound enzymes and to increase the release from the matrix lysosomes. estradiol proved less active than is testosteron. the renal lysosomes in vitro ... | 1978 | 97095 |
| induction of arylhydrocarbon hydroxylase and udp glucuronosyl transferase by pcb in hepatic cell cultures. | the purpose of the investigation was to derive a hepatic cell line to be used in induction studies of drug metabolizing enzymes. two pure cell lines were isolated from primary liver cell cultures, one with an epithelial-like appearance, the other with a fibroblast-like appearance. the specific activities of arylhydrocarbon hydroxylase (ahh) and udp glucuronosyl transferase (udpgt) were greater in hepatocyte cultures than in primary cultures or in fibroblast cultures. pcb enhanced the activity of ... | 1978 | 98133 |
| [immuno-electro-diffusion on cellulose acetate membrane for serological testing in human malaria (author's transl)]. | an immuno-electro-diffusion assay for human malaria, using cellulose acetate gel, is described. more than 3500 sera have been tested by this method, which proved very useful for epidemiology since 400 sera a day can be tested by one person, with simple equipment. its sensitivity compared well to the ifa test, although the antibodies revealed are quite different. a constnat precipitin line appeared, against 56 degrees c labile antigens, in sera of subjects infected by p. falciparum, and up to 8 o ... | 1978 | 98748 |
| guinea pig immunoglobulin light chain isotypes. i. separation of kappa and lambda chains and the identification of three isotypes of the lambda chain constant homology region. | the separation of intact kappa chain and two fragments comprising lambda (lambda) chain from immunoglobulin light chain pools isolated from strain 13 guinea pigs is achieved by cyanogen bromide digestion and gel filtration before and after reductive clevage of disulfide bonds. the smaller lambda chain fragment derives from the original carboxyl-terminus of lambda chain. the partial sequence of component tryptic and thermolytic peptides of this thirty-nine residue fragment allowed its complete se ... | 1978 | 103729 |
| transfer of 2,4,5,2',4',5'-hexachlorobiphenyl and 2,2-bis-(p-chlorophenyl),1,1,1-trichloroethane (p,p'-ddt) from maternal to newborn and suckling rats. | pregnant rats were given a small dose of 14c-2,4,5,2',4',5'-hexachlorobiphenyl (hcb) and 3h-ddt intraperitoneally. the transfer of hcb and ddt through the placenta and milk was then investigated. transfer through the placenta was 2.7 and 1.5% (respectively) of the initial doses; transfer through milk was 39.2 and 21.5%. hcb is obviously more transferable than ddt through the placenta and milk, the ratio of the amount of hcb transferred through milk to the amount transferred through the placenta ... | 1978 | 104695 |
| specificity and biological distribution of coenzyme m (2-mercaptoethanesulfonic acid). | the specificity of the growth requirement of methanobacterium ruminantium strain m1 for a new coenzyme, 2-mercaptoethanesulfonic acid (hs--com), was examined. a variety of derivatives, analogs, and potential biosynthetic precursors of coenzyme m were tested; only a restricted range of thioether, thioester, and thiocarbonate derivatives of the cofactor were found to replace the hs--com requirement. bromoethanesulfonic acid (brch2ch2so3-), a halogenated analog of hs--com, potently inhibited the gr ... | 1979 | 104960 |
| specific labelling of replicating spp1 dna: analysis of viral dna synthesis and identification of phage dna-genes. | specific labelling of replicating bacteriophage spp1 dna can be achieved by infection at nonpermissive temperature of a b. subtilis strain carrying the initation mutation dnab ts134. under these conditions host dna synthesis is reduced by 90 to 95%. this technique was used to identify cistrons of spp1 involved in phage dna synthesis and to define intermediates in spp1 replication. | 1978 | 105242 |
| a model of biliary pancreatic reflux. | a primate model for the study of biliary pancreatic reflux under relatively physiological conditions is described. cannulas were inserted into the gallbladder and the common bile duct of rhesus monkeys, and a pedicled segment of small bowel was used to create a pancreaticocutaneous fistula after resection of the spleen and pancreatic tail. following recovery, hypaque was instilled into the gallbladder with maintenance of common duct pressure within a normal range. the pancreatic duct was visuali ... | 1979 | 105415 |
| interference of iodine-125 ligands in radioimmunoassay: evidence implicating thyroxine-binding globulin. | although there is abundant published evidence that radioiodinated antigens interfere in digoxin radioimmunoassays, other radioimmunoassays are similarly affected. we investigated the relationship of radioiodinated antigen structure to its function in the immunoassay. carrier-free 125i-labeled iodotyrosine and iodohistamine derivatives were incubated with human serum, and the bound and free fractions were separated. we demonstrated that diiodotyrosyl analogs bind avidly to serum proteins. because ... | 1979 | 108034 |
| effect of morphine on intracranial self-stimulation behavior following brain amine depletion. | | 1975 | 124807 |
| ultrastructural aspects of cardiac lymphatic capillaries in experimental cardiac hypertrophy. | | 1975 | 124810 |
| flaujeac trait. deficiency of human plasma kininogen. | flaujeac trait plasma resembled hageman trait or fletcher trait, in that the intrinsic coagulation pathway, plasma fibinolytic pathway, kinin-forming system, permeability factor of dilution (pf/dil) phenomenon were abnormal. the defect in each assay was reconstituted by afactor separable from hageman factor or fletcher factor. this substance was an alpha-globulin with an approximate mol wt of 170,000. flaujeac plasma did not release a kinin upon incubation with kallikrein and was deficient in to ... | 1975 | 127805 |
| the nature of the protein moieties of cartilage proteoglycans of pig and ox. | proteoglycans extracted with 4m-guanidinium chloride from pig laryngeal cartilage and bovine nasal septum were purified by density-gradient centrifugation in cscl under 'associative' followed by 'dissociative' conditions [hascall & sajdera (1969) j. biol. chem. 244, 2384-2396]. proteoglycans were then digested exhaustively with testicular hyaluronidase, which removed about 80% of the chondroitin sulphate. the hyaluronidase was purified until no proteolytic activity was detectable under the condi ... | 1975 | 128355 |
| inhibitory effects of mersalyl and of antibodies directed against smooth-muscle myosin on a calcium adenosine triphosphatase of the plasma membrane from mouse liver cells. | | 1976 | 130830 |
| [morphological changes in the digestive organs during prolonged space flight on the kosmos-782 biosatellite]. | a reduction in the content of neutral mucopolysaccharides in mucous cells of the neck, a slight decrease in the activity of succinate dehydrogenase and nad-diaphorase in parietal cells, a decrease in the dna synthesis rate, and an increase in the area of mitochondria and cristae were detected in the gastric mucosa of rats which were in a long-term space flight. in the small intestine, an increase in the activity of glucose-6-phosphate dehydrogenase and leucine aminopeptidase were found. morpholo ... | 1978 | 152100 |
| [intravenous cholangiography in hyperbilirubinaemia (author's transl)]. | the effect of bilirubin on biliary and renal excretion of methylglucamine-iodipamide at an infusion rate of 1.3, 2.6 and 5.2 mu mol/min/kg was studied by intravenous injection into five dogs with normal liver functions and chronic duodenal fistulas. unconjugated bilirubin administered in a dose of 0.1 mu mol/min/kg reduced biliary iodipamide excretion and concentration significantly, but did not affect renal contrast material excretion. this indicates that the unsatisficatory radiological result ... | 1979 | 153309 |
| sex specific oestrogen binding in the middle hypothalamus of rats. | | 1975 | 179802 |
| malignant transformation-linked imbalance: decreased xanthine oxidase activity in hepatomas. | xanthine oxidase was decreased 2- to 10-fold in all examined rat hepatomas irrespective of the malignancy; growth rate and degrees of histological differentiation of the neoplasms. the affinity to substrate (km=6-8 mum) and the ph optimum (8.0) of the liver and hepatoma enzymes were the same. the reprogramming of gene expression, as manifested in the decreased activity of this key purine metabolizing enzyme, appears to be specific to neoplastic transformation. since glutamine prpp amidotransfera ... | 1975 | 179860 |
| structural studies and physical maps for the herpes simplex virus genome. | | 1977 | 193820 |
| [study of cytoplasmic rna in tissues of the rat, regenerating liver and in liver of rats with hepatoma pc-1]. | cytoplasmic rna of the rat regenerating liver and liver of rats with hepatoma pc-1 is separated into five fractions on the column of kieselgur with methylated albumin (mak). studies in the nucleotide composition showed that certain fractions of cytoplasm rna are ribosomal and messenger rna. the highest amount of radioactive precursor is incorporated into messenger rna of the rat regenerating liver and liver of the rats with hepatoma pc-1 which is eluated from the column by 0.2% solution of sodiu ... | 1977 | 201068 |
| the presence of disulfide-linked gp70-p15(e) complexes in akr murine leukemia virus. | | 1977 | 201090 |
| influence of adrenocorticotropin on transport of a cholesteryl linoleate-low density lipoprotein complex into adrenal tumor cells. | the action of adrenocorticotropin (acth) on the specific (receptor-mediated) uptake of cholesteryl linoleate . low density lipoprotein complexes was examined in y-1 mouse adrenal tumor cells. high affinity binding (ka 4.1 x 10(8) m) was observed with acth; lower affinity was seen in the absence of acth. the effect of acth was observed within 10 min at physiological concentrations of low density lipoprotein (100 microgram/ml). binding was followed by uptake (internalization) of the ester . lipopr ... | 1979 | 227899 |
| a slowly dissociating form of the cell surface cyclic adenosine 3':5'-monophosphate receptor of dictyostelium discoideum. | experiments using a phosphodiesterase-minus mutant of dictyostelium discoideum indicate that ligand-induced loss of cell surface cyclic adenosine 3':5'-monophosphate binding sites (down regulation) can be evoked with concentrations of cyclic adenosine 3':5'-monophosphate as low as 10(-8) m. the loss of receptor sites is observed after 5 min of cell preincubation with cyclic adenosine 3':5'-monophosphate and can be as extensive as 75 to 80%. this decrease in binding sites is correlated with the a ... | 1979 | 227901 |
| marek's disease lymphoma of chicken and its causative herpesvirus. | | 1979 | 228727 |
| isolation and partial biochemical characterization of the brush border plasma membrane from the cestode, hymenolepis diminuta. | | 1979 | 229204 |
| effects of althesin and its steroidal components on a variety of excised smooth muscle preparations. | | 1979 | 262472 |
| influence of the substituent radicals in antimonyl naphthalein derivatives on the potentialities of anti-schistosomal activity in mice. | | 1979 | 262474 |
| the measurement of bile acid-dependent and independent fractions of rat bile. | bile flow and bile acid secretory rate in rate in rats with interruption of the enterohepatic circulation had a biphasic decay with the second slower slope less marked for bile flow. a direct relationship between both parameters was observed in these animals. by knowing the choleretic efficiency of bile acids calculated from the regression line, the total bile acid output and the volume of bile collected, the fraction of bile produced by other way than bile water carried by bile acids was estima ... | 1978 | 263162 |
| surface ultrastructural response of the human uterine lining epithelium to hormonal environment. a scanning electron microscopic study. | | 1977 | 269610 |
| therapy for infections due to anaerobic bacteria: an overview. | several classes of antimicrobial agents (e.g., penicillins, cephalosporins, tetracyclines, chloramphenicol, and clindamycin) are useful in treatment of infections due to anaerobic bacteria. however, certain anaerobic bacteria have shown a striking resistance to antimicrobial agents. in vitro susceptibility tests are useful for selection of optimal therapy. the choice of agent depends, to some extent, on the organisms responsible for the infection. bacteroides fragilis is the most commonly encoun ... | 1977 | 321708 |
| [the ovary and the immunity system]. | | 1977 | 340059 |
| human bone marrow colony formation in diffusion chambers. normal values. | | 1978 | 340240 |
| nucleotide sequence of the ampicillin resistance gene of escherichia coli plasmid pbr322. | i have determined the nucleotide sequence of the ampicillin resistance gene of pbr322, an escherichia coli plasmid that encodes a penicillin beta-lactamase. this gene codes for a protein of 286 amino acid residues. the first 23 amino acids presumably form a signal for secretion, because they do not appear in the mature enzyme, whose partial amino acid sequence has been determined independently. | 1978 | 358200 |
| quantitative estimation of leishmanial antibody titers by enzyme-linked immunosorbent assay. | the response to immunization against leishmania braziliensis in rabbits was followed by measurement of antibody by enzyme-linked immunosorbent assay (elisa), passive hemagglutination, complement fixation, and countercurrent immunoelectrophoresis. passive hemagglutination and complement-fixation titers were interpreted according to standard methods, whereas elisa exact titers were derived by the equation y = a-b log x, which describes the straight line that results when absorbances are plotted ag ... | 1979 | 387886 |
| [dynamics of the development of different microorganisms in soil]. | the dynamics of individual components from the complex of soil microorganisms (bacteria, fungi, actinomycetes) and the population of stm. olivocinereus introduced into soil was studied by luminescent microscopy and inoculation. the population density maxima for individual components from the complex of soil microorganisms were shown to be separated in time suggesting a succession. fungi developed at the first steps of succession and dominated in biomass over the remaining components of the compl ... | 1979 | 470632 |
| ehrlichiosis in wolves, dogs, and wolf-dog crosses. | canine ehrlichiosis was diagnosed in wolves, dogs, and wolf-dog crosses at a small zoo in northcentral florida. five of 9 adult canids and all 8 pups confined to a common kennel died as a result of the infection. the epizootic was associated with a massive rhipicephalus sanguineus infestation. hematologic and pathologic findings in an adult wolf-dog cross that died were characteristic of canine ehrlichiosis. that animal and four other canids were seropositive for ehrlichia canis. no deaths occur ... | 1979 | 521367 |
| the brown dog tick rhipicephalus sanguineus and the dog as experimental hosts of ehrlichia canis. | adult rhipicephalus sanguineus ticks efficiently transmitted ehrlichia canis to susceptible dogs for 155 days after detachment as engorged nymphs from a dog in the acute phase of ehrlichiosis. adult ticks that had similarly engorged on a dog in the chronic phase of ehrlichiosis failed to transmit e canis to susceptible dogs. infected but unfed adult ticks may be of greater importance than the chronically infected carrier dog as a natural reservoir of e canis. | 1977 | 596693 |
| retrospective study of an ehrlichia canis epizootic around phoenix, arizona. | antibodies to ehrlichia canis were detected by indirect immunofluorescence in sera from 40 of 339 dogs (12%) examined from around phoenix, az, during 1975. the prevalence was higher in purebred and crossbred german shepherd dogs (21%), as compared with 8% in the remaining breeds. the difference in antibody prevalence between sexes was not significant. dogs 12 weeks to 13 years old were seropositive. the causative agent was isolated by inoculation of whole blood from a seropositive dog into a sus ... | 1978 | 624663 |
| effect of canine immune serum on the growth of ehrlichia canis within nonimmune canine macrophages. | the effect of canine immune serum (cis) on the growth of ehrlichia canis was studied in macrophage cultures derived from peripheral blood monocytes of normal dogs. ehrlichiae treated with canine normal serum and then introduced into normal macrophage cultures maintained in canine normal serum multiplied within the macrophages and destroyed them. immune serum, collected from e canis carrier dogs, suppressed ehrlichial growth in normal macrophages. an inverse relationship existed between the amoun ... | 1978 | 629451 |
| effect of canine immune macrophages and canine immune serum on the growth of ehrlichia canis. | the effect of canine immune macrophages and canine immune serum (cis) on the growth of ehrlichia canis was studied in vitro. treatment of e canis organisms with cis before they were inoculated into immune macrophage cultures, coupled with pre- and postinoculation maintenance of cultures in cis, suppressed macrophage infection rates to 1% or less. immune macrophages collected from e canis-carrier dogs and maintained in canine normal serum were more resistant to the intracellular growth of e canis ... | 1978 | 629452 |
| prophylactic and therapeutic use of tetracycline during an epizootic of ehrlichiosis among military dogs. | an epizootic of canine ehrlichiosis at the military working dog center, pakchong, thailand, was arrested by prophylactic and therapeutic use of tetracycline and by control of ticks. oral treatment with tetracycline (66 mg/kg of body weight daily for 14 days) effected remission of signs of disease in 60 of 62 clinically affected dogs and resulted in disappearance of serum antibody in 119 (60%) of 198 seropositive dogs treated during the 2-year study period. transmission of ehrlichia canis was int ... | 1978 | 640933 |
| autologous lymphocyte-mediated cytotoxicity against monocytes in canine ehrlichiosis. | the 51cr-release technique for detecting cytotoxicity had been adapted to immunologic studies of canine ehrlichiosis. lymphocytes from dogs infected with ehrlichia canis, cause of canine ehrlichiosis, were shown to be cytotoxic for autologous monocytes. the effect was dependent on the concentraton of lymphocytes, being optimal at 100:1 lymphocyte:monocyte ratio. neither immune serum and complement, nor anti-canine globulin had any observable effect on cytotoxicity. the monocytotoxicity bore a te ... | 1977 | 931138 |
| canine peritoneal macrophages: cultivation and infection with ehrlichia canis. | canine peritoneal macrophages were obtained by repeated peritoneal lavage of dogs at 14 day intervals. intraperitoneal administration of sterile mineral oil increased the leukocyte yield approximately 20-fold and the macrophage recovery approximately 35-fold. cell recovery was maximum 7 to 21 days after administration of oil and declined slightly by 35 days. restimulation with a 2nd injection of oil promptly revitalized cell recovery. peritoneal macrophage cultures were well established by 6 day ... | 1977 | 931165 |
| acute canine ehrlichiosis: platelet survival and factor 3 assay. | ten dogs experimentally inoculated with ehrlichia canis were thrombocytopenic 14 days after inoculation. circulating platelet concentrations had declined rapidly by postinoculation day 10, but remained at or slightly below the 14-day concentration during the remainder of the 42-day experimental period. the percentage of circulating megathrombocytes also increased to 8.7 times preinoculation concentrations indicating the existence of accelerated thrombocytopoiesis. the release of platelet factor ... | 1977 | 931166 |
| development of ehrlichia canis, causative agent of canine ehrlichiosis, in the tick rhipicephalus sanguineus and its differentiation from a symbiotic rickettsia. | certain aspects of the development of ehrlichia canis, causative agent of canine ehrlichiosis (tropical canine pancytopenia) in rhipicephalus sanguineus ticks were studied. it was found that partial feeding of nymphs infected as larvae with e canis was a desirable, if not necessary, preliminary treatment for successful infection of dogs with ground-up ticks. it remains unclear whether feeding increased the number or altered the virulence of ehrlichiae within tick tissues. ehrlichia canis organis ... | 1976 | 944000 |
| pre- and postnatal enzyme capacity for drug metabolite production. | most lipid-soluble foreign compounds including drugs, insecticides and many environmental pollutants are metabolized in animals by cytochrome p-450 enzymes in the endoplasmic reticulum of liver. these enzymes are virtually absent in fetuses of laboratory animals, but their activities increase to adult levels within 3-8 weeks after birth. in human fetuses, the enzymes appear during the first half of pregnancy, and their activities during gestation reach about one third of those found in adults. t ... | 1975 | 1090453 |
| identification of ehrlichia canis in east africa. | ehrlichia canis, the causative agent of canine ehrlichiosis or tropical canine pancytopaenia (tcp) has been identified in kenya. transmission studies to two dogs resulted in signs of ehrlichiosis including marked thrombocytopaenia, pyrexia, reduction in the packed cell volume and the presence of e canis in peripheral blood mononuclear cells. | 1976 | 997191 |
| platelet kinetics in canine ehrlichiosis: evidence for increased platelet destruction as the cause of thrombocytopenia. | a significant (p < 0.025) increase in the mean platelet diameter occurred in five ehrlichia canis-infected dogs when platelet numbers decreased to 100,000/mul or less. maximal incorporation of [(75)se]selenomethionine into platelets of six uninfected dogs was 0.080 +/- 0.019% (mean +/- standard error) and occurred 5 to 6 days after dosage, whereas maximal incorporation was 0.036 +/- 0.004% within 2 to 3 days after dosage in seven chronically infected dogs that had thrombocytopenia. analysis of t ... | 1975 | 1140846 |
| transmission of ehrlichia canis to dogs by ticks (rhipicephalus sanguineus). | two strains of rhipicephalus sanguineus acquired ehrlichia canis by feeding as either larvae or nymphs on acutely infected dogs and, in subsequent instars, transmitted the agent to normal dogs. three strains of r sanguineus transmitted e canis as adults after their larval and nymphal stages fed on infected dogs. more than 400 adult female ticks were fed on infected dogs as larvae or nymphs or both, but none transmitted e canis transovarially. | 1975 | 1147359 |
| ehrlichia canis infection in a dog in virginia. | | 1975 | 1150498 |
| ehrlichia canis infection in a child in south carolina. | | 1992 | 1319878 |
| combined effects of ethanol and protein deficiency on hepatic iron, zinc, manganese, and copper contents. | the present study has been performed in order to establish the relative and combined roles of ethanol and malnutrition on liver fe, zn, cu, and mn alterations in alcoholic male adult wistar rats, and also the relationships between these alterations and histomorphometrically determined hepatocyte and nuclear areas, perivenular fibrotic rim area, and total amount of fat present in the liver. four groups of 8 animals each were fed: (1) a nutritionally adequate diet (c); (2) a 36% ethanol-containing ... | 1992 | 1418656 |
| characterization of disulfide-linked heterodimers containing apolipoprotein d in human plasma lipoproteins. | human plasma apolipoprotein (apo) d is a glycoprotein with an apparent molecular weight of 29,000 m(r). it is present, mainly, in high density lipoproteins (hdl) and very high density lipoproteins (vhdl). western blot analysis of hdl and vhdl using rabbit antibodies to human apod revealed major immunoreactive bands at 29,000 and 38,000 m(r), with minor bands ranging from 50,000 to and 80,000 m(r). only the 29,000 m(r) band corresponding to apod remained when the electrophoresis was conducted und ... | 1992 | 1479288 |
| ehrlichia ewingii sp. nov., the etiologic agent of canine granulocytic ehrlichiosis. | the 16s rrna gene was amplified, cloned, and sequenced from the blood of two dogs that were experimentally infected with the etiologic agent of canine granulocytic ehrlichiosis. the 16s rrna sequence was found to be unique when it was compared with the sequences of other members of the genus ehrlichia. the most closely related species were ehrlichia canis (98.0% related) and the human ehrlichiosis agent (ehrlichia chaffeensis) (98.1% related); all other species in the genus were found to be phyl ... | 1992 | 1581189 |
| investigation of renal protein loss in dogs with acute experimentally induced ehrlichia canis infection. | urinary protein-to-creatinine ratios and serum albumin concentrations were measured in 8 adult male dogs experimentally inoculated with ehrlichia canis. urinary protein concentration increased significantly, but transiently, during the acute phase of infection. urinary protein-to-creatinine ratios were highest (mean, 8.6) during the third and fourth weeks after infection, and decreased to less than 0.5 by 6 weeks after infection. correspondingly, albumin concentration decreased significantly dur ... | 1992 | 1595954 |
| antigenic and genomic relatedness among ehrlichia risticii, ehrlichia sennetsu, and ehrlichia canis. | antigenic and genomic relatedness among ehrlichia risticii, e. sennetsu, and e. canis was analyzed by enzyme-linked immunosorbent assay, western blotting (immunoblotting) and dna-dna hybridization. e. risticii and e. sennetsu were serologically related, and their western blot antigen profiles were nearly identical. two antigens of e. sennetsu corresponding to the 28- and 51-kda antigens of e. risticii were apparently larger than the e. risticii antigens, and the 55-kda antigen of e. risticii app ... | 1992 | 1736961 |
| rickettsiae and borrelia burgdorferi in ixodid ticks. | nymphs and adults of hard-bodied ticks were collected in connecticut and tested by direct and indirect immunofluorescence staining methods for rickettsiae and borrelia burgdorferi. of the 609 ixodes dammini ticks examined, 59 (9.7%) harbored rickettsialike microorganisms in hemocytes (blood cells). these bacteria reacted with fluorescein-conjugated antiserum to ehrlichia canis, the etiologic agent of with fluorescein-conjugated antiserum to ehrlichia canis, the etiologic agent of canine ehrlichi ... | 1991 | 1757551 |
| development of an in vitro microtest to assess drug susceptibility of babesia bovis and babesia bigemina. | continuous cultivation of the bovine hemoparasites babesia bovis and babesia bigemina was developed as an in vitro microtest to assess parasite susceptibility to babesicidal compounds. reproducibility of parasite multiplication rates was independent of culture size, making it possible to use a microscale of 100 microliters for each test sample. inhibitory concentrations (ic50s) of a commonly used babesicide, quinuronium sulfate, evaluated by this in vitro method were found to be 5 x 10(-8) g/ml ... | 1991 | 1779305 |
| altered coupling states between calcium transport and (ca2+, mg2+)-atpase in the as-30d ascites hepatocarcinoma plasma membrane. | plasma membrane fractions from normal, regenerating liver and the as-30d ascites hepatocarcinoma exhibited a high degree of enrichment when a set of plasma membrane enzyme markers were studied in comparison to the ones associated to the mitochondrial and cytosolic compartments. while the (ca2+, mg2+)-atpase observed for the plasma membrane fraction isolated from normal liver showed an activity of 1.2 mumoles/mg/min, the regenerating liver and the as-30d plasma membrane fractions presented a much ... | 1991 | 1828860 |
| lovastatin inhibits gallstone formation in the cholesterol-fed prairie dog. | the efficacy of lovastatin, an inhibitor of hepatic cholesterol synthesis in the prevention of cholesterol gallstone formation, was evaluated in the prairie dog model. two groups of animals were maintained on either nonlithogenic or 1.2% cholesterol-enriched chow for 21 days. seven of the animals in each group received lovastatin, and the remaining six received only distilled water. all of the cholesterol-fed/water-treated animals had crystals and 83% had gallstones, but none of the cholesterol- ... | 1991 | 1867522 |
| canine ehrlichiosis in six dogs with persistently increased antibody titers. | chronic ehrlichiosis was diagnosed in six dogs on the basis of increased immunofluorescent antibody titers to ehrlichia canis. although clinical signs varied, all six dogs were anemic, hyperglobulinemic, and an igg monoclonal gammopathy was documented in five dogs in which serum protein electrophoreses were performed. all dogs were treated with tetracycline for at least 14 days; four dogs also received immunosuppressive drugs. clinical signs resolved within 1 week, hematologic abnormalities reso ... | 1991 | 1920256 |
| hemophagocytic syndrome: a cause of pancytopenia in human ehrlichiosis. | a 67-year-old white man with human ehrlichiosis infection complicated by pancytopenia, hemophagocytic syndrome, disseminated intravascular coagulopathy and septic shock is presented. the patient had been on a three-week camping trip to california, colorado, utah, and new mexico. the diagnosis of human ehrlichiosis was confirmed by sixteen-fold rise in antibody titer to ehrlichia canis, and supported by the characteristic cytoplasmic inclusions. human ehrlichiosis should be considered in the diff ... | 1991 | 1951324 |
| serologic diagnosis of human ehrlichiosis using two ehrlichia canis isolates. | ehrlichia canis or a closely related rickettsial organism has been implicated serologically and morphologically as the causative agent of human ehrlichiosis in the united states. although e. canis has been serially propagated in primary canine monocytes, only a limited quantity of antigen is obtained by this method. a continuous canine macrophage cell line, dh82, supports the growth of a new isolate of e. canis established from the whole blood of a carrier dog in oklahoma. serologic comparison o ... | 1991 | 1995730 |
| serological evaluation of ehrlichia canis infections in military dogs in africa and reunion island. | sixty-eight dogs from four african countries and reunion island were tested for antibodies against ehrlichia canis. twenty-six dogs (50%) in tunisia, senegal and chad were found positive using the indirect fluorescence antibody test. dogs from both the central african republic and reunion island were all negative. thus, this preliminary report confirms the presence of e. canis in africa. larger studies will be necessary to evaluate the current epidemiologic situation of canine ehrlichiosis in th ... | 1991 | 2024432 |
| antiarrhythmic properties of benzyl-triamterene derivatives in the coronary artery ligated and reperfused rat. | triamterene (cas 396-01-0) and a series of benzyl-triamterene derivatives were evaluated for their antiarrhythmic properties in the coronary artery ligated and reperfused (cal-r) rat. the effects were compared with the antiarrhythmic activity of the potassium sparing diuretic amiloride and drugs out of the class-i (lidocaine) and class-iii (amiodarone and sotalol). triamterene and sotalol revealed at high doses antifibrillator activity, while the benzyl-triamterenes 2, 3, 5 and 6 could also depr ... | 1991 | 2043173 |
| immunohistologic demonstration of ehrlichia canis. | | 1990 | 2165753 |
| diagnosis of human ehrlichiosis with the indirect fluorescent antibody test: kinetics and specificity. | human ehrlichiosis, an acute febrile illness caused by ehrlichia canis or a closely related rickettsial organism, was first identified in 1986. from 1986 through 1988, sera from 85 patients demonstrated a fourfold rise or fall in antibody titer to e. canis. seven (22%) of 32 patients initially tested during the first week after onset of illness. 17 (68%) of 25 tested during the second week, and all 18 tested during the third week had titers that exceeded the minimum positive titer of greater tha ... | 1990 | 2192013 |
| ehrlichial diseases of dogs. | ehrlichial diseases of dogs currently are recognized to be caused by ehrlichia canis, ehrlichia platys, ehrlichia equi, and ehrlichia risticii. information on each disease's history, transmission, pathogenesis, clinicopathologic findings, diagnosis, therapy, and prevention is presented in this article. ehrlichia canis is the most common cause of clinical illness. case reports are included to illustrate common presentations of ehrlichia canis infections. | 1991 | 2014630 |
| evaluation of rhipicephalus sanguineus as a potential biologic vector of ehrlichia platys. | the brown dog tick, rhipicephalus sanguineus (acari: ixodidae), transmits several diseases among dogs including ehrlichia canis infection. the role of rhipicephalus sanguineus as a biologic vector for e platys, the rickettsial agent of infectious canine cyclic thrombocytopenia, was studied in dogs. laboratory-cultured, pathogen-free nymph ticks were fed to repletion on dogs acutely infected with e platys. tick engorgement coincided with the development of initial parasitemia and thrombocytopenia ... | 1991 | 1952347 |
| antigenic analysis of four species of the genus ehrlichia by use of protein immunoblot. | the antigenic profile of ehrlichia canis, e risticii, e sennetsu, and e equi was investigated by the use of protein (western) immunoblot technique. results of analysis of serum from acutely and chronically infected animals indicated that the 4 ehrlichia species share a unique 25-kd polypeptide in addition to other peptides. immune sera from dogs inoculated with e canis recognized a wide range of e canis polypeptide antigens, as determined by western blot analysis. a larger number of e sennetsu p ... | 1991 | 1928904 |
| [autosomal recessive oculopharyngeal "muscular dystrophy"--clinical features and association with reduced activity of myophosphorylase]. | we reported two cases of brothers demonstrating oculopharyngeal muscular dystrophy (opmd). the cases had consanguineous parents and five healthy siblings, which suggested the autosomal recessive inheritance. the initial symptom was slowly progressive blepharoptosis with onset in the third decade. on examination, total external ophthalmoplegia was observed in both patients. additionally, the elder, a 57-year-old man, exhibited dysarthria, dysphagia and muscular weakness with atrophy of the face, ... | 1991 | 1914322 |
| molecular cloning of ehrlichia risticii and development of a gene probe for the diagnosis of potomac horse fever. | a gene bank of ehrlichia risticii was constructed in plasmid vector puc13. five clones representing discrete regions of the e. risticii genome were tested for their ability to hybridize specifically to e. risticii dna. none of the clones cross-hybridized with ehrlichia equi dna, whereas four of these clones cross-hybridized with ehrlichia canis and ehrlichia sennetsu dnas. however, one clone carrying a 1-kilobase hindiii fragment of e. risticii dna failed to cross-react with the genomes of e. se ... | 1990 | 2229378 |
| [carbonic anhydrase iii--a marker of the myoepithelial cells of human salivary glands]. | carbonic anhydrase iii (caiii) is the isoenzyme purified from the human skeletal slow muscle immunohistochemically revealed in smooth muscle cells of uterus, myoepithelial cells of salivary, lactiferous and prostatic glands of man. immunohistochemical determination of caiii is a useful approach to correct the identification of myoepithelial cells in human salivary glands. | 1991 | 1905166 |
| concurrent babesiosis and ehrlichiosis in the dog: blood smear examination supplemented by the indirect fluorescent antibody test, using cowdria ruminantium as antigen. | giemsa-stained, peripheral blood smears of 67 dogs, showing clinical signs typical of babesiosis or reminiscent of concurrent babesiosis and ehrlichiosis, were examined for the presence of babesia canis and ehrlichia canis. since cowdria ruminantium cross-reacts with ehrlichia, the sera of these dogs were also subjected to the indirect fluorescent antibody (ifa) test in which c. ruminantium was used as antigen. fifty-five per cent of these dogs had mixed infections of b. canis and e. canis, as j ... | 1990 | 2234860 |
| neurologic manifestations of trypanosomiasis in a dog. | a 13-month-old doberman pinscher was evaluated because of slowly progressive paraparesis and signs of depression. the dog had temporal, supraspinatus, and infraspinatus muscle atrophy, bilateral enophthalmos, superficial inguinal lymphadenopathy, tachycardia with pulse deficits, and lesions of active and inactive chorioretinitis. neurologic abnormalities included hyperreflexic patellar reflexes, lack of conscious proprioception, signs of superficial pain in the hind limbs, and depressed hopping ... | 1991 | 1899857 |