nonautotrophic thiobacillus in acid mine water.nonautotrophic thiobacilli were isolated from the acidic water of a coal mine. based on their mixotrophic physiology, the isolates are regarded as strains of thiobacillus perometabolis.1975954
new medium for isolating iron-oxidizing and heterotrophic acidophilic bacteria from acid mine drainage.a new solid medium is described for growing iron and heterotrophic bacteria from acid mine drainage (amd). examination of amd from five states revealed several kinds of colonies of iron-oxidizing bacteria: (i) smooth, (ii) smooth with secondary growth sectors or branching, (iii) star-shaped, (iv) radiating lobe, and (v) flat-rough. all amd samples yielded whitish colonies that could not use ferrous iron, sulfur, or hydrogen, nor could they grow on nutrient agar, brain heart infusion agar, or try ...19752103
ferrous ion oxidation and uranium solubilization from a lowgrade ore by "thiobacillus ferrooxidans" (author's transl).the microbiological oxidation of ferrous ion and the extraction of uranium from a low-grade ore has been studied using an adapted strain of thiobacillus ferrooxidans. the effect of temperature, ph, volumetric oxygen transfer coefficient, k1a, and aeration number, ia, on the activity of the microorganism has been determined. the activation energy for ferrous iron oxidation was calculated to be - 13.9 +/- 0.1 kcal/mole and inactivation (thermal death of bacteria) 53.3 +/- 0.2 kcal/mole. temperatur ...19753131
ribulose biophosphate carboxylase from thiobacillus a2. its purification and properties.ribulose bisphosphate carboxylase (ec from thiobacillus a2 has been purified to homogeneity on the basis of polyacrylamide gel electrophoresis and u.v. analysis during sedimentation velocity studies. the enzyme had an optimum ph of about 8.2 with tris-hcl buffers. the molecular weight was about 521000 with an srel. of 16.9. km for rubp was 122 mum, for total "co2" it was 4.17 mm, and for mg2+ 20.0 mum. the absolute requirement for a divalent cation was satisfied by mg2+ which was repla ...19765983
inhibition of growth, iron, and sulfur oxidation in thiobacillus ferrooxidans by simple organic compounds.iron and sulfur oxidation by thiobacillus ferrooxidans as well as growth on ferrous iron were inhibited by a variety of low molecular weight organic compounds. the influences of chemical structure of the organic inhibitors, ph, temperature, physical treatment of cells, and added inhibitory or stimulatory inorganic ions and iron oxidation suggest that a major factor contributing to the inhibitory effects on iron oxidation is the relative electronegativity of the organic molecule. the data also su ...19766140
the respiratory chain of thiobacillus ferrooxidans: the reduction of cytochromes by fe2+ and the preliminary characterization of rusticyanin a novel "blue" copper protein. 19756319
alkaline phosphatase of thiobacillus thioparus. partial purification and properties of the enzyme.soluble alkaline phosphatase from thiobacillus thioparus cells was purified about 230-fold. the enzyme had a mol. wt. of 50 000 daltons, optimum ph at 10.5, and was heat-resistant in the presence of diethanolamine. polyacrylamide-gel electrophoresis demonstrated contamination of the preparation with inactive proteins and the presence of two active bands. the enzyme activity was distinctly stimulated by increasing concentrations of tris or diethanolamine. in the presence of glycine, 1 mm-zn2+ enh ...19767090
microbiological oxidation of synthetic chalcocite and covellite by thiobacillus ferrooxidans.the microbiological oxidation of synthetic chalcocite and covellite has been investigated using an adapted strain of thiobacillus ferrooxidans. biodegradation of chalcocite was found to be 90 to 100% and that of covellite 45 to 60%. optimum conditions for the oxidation of chalcocite were: ph, 1.7 to 2.3; temperature, 35 c; and ferric iron concentration in the range of 0.004 to 0.01 m. for covellite, the optimum conditions were: ph 2.3; temperature, 35 c; and ferric iron concentration in the rang ...19768006
ecology of thiobacillus neapolitanus and its possible role in the leaching process. 19769736
thermothrix thioparus gen. et sp. nov. a facultatively anaerobic facultative chemolithotroph living at neutral ph and high temperature.thermothrix thioparus gen. et ep. nov. occurs naturally in a new mexico hot spring at a temperature of 74 degrees c, a ph of 7.0, and a hs- concentration of 1 mg/litre. the organism is gram-negative, non-motile, 0.5-1.0 x 3-20 mum, and forms cell chains up to 1 cm in length. the resulting filaments do not possess a sheath. sulfur is deposited extracellularly. the organism was isolated using an autotrophic medium with hs- as the energy source and no3- as the terminal electron acceptor. anaerobica ...197610063
utilization of 35s-thiosulphate and an appraisal of the role of atp-sulphurylase in chemolithotrophic thiobacillus ferrooxidans.differentially labelled 35s-thiosulphate was taken up by washed cells of thiobacillus ferrooxidans which were previously grown on thiosulphate. the uptake was proportional to the biomass over the range 0.5-4.0 mg dry wt. of bacteria and showed typical saturation kinetics with an estimated km value of 0.5 mm for 35s-thiosulphate. dithionate and group vi anions inhibited the uptake, which was under ph control and had a temperature optimum of 50 degrees c. in the absence of thiosulphate, the cells ...197610869
rate of elemental sulfur oxidation in some soils of egypt as affected by the salinity level, moisture, texture, temperature and inoculation.1. the level of soil moisture most favourable to the oxidation of elemental sulfur was found to be around the field moisture capacity; the oxidation rate decreased at lower and higher moisture levels. 2. the rate of oxidation increased with the clay content of the soils from sandy loam to clay loam. 3. although s-oxidation could be found already at 10 degrees c, it increased markedly up to a maximum at 35 degrees c and then decreased again at higher temperatures. 4. increasing the salt content o ...197611771
rhodanese from thiobacillus a2: catalysis of reactions of thiosulphate with dihydrolipoate and dihydrolipoamide.rhodanese (thiosulphate:cyanide sulphurtransferase ec. was purified 25- to 30-fold from thiosulphate-grown thiobacillus a2. it exhibited a ph optimum between ph 10-2 and 10-4 and apparent km values of 0-36 mm-na2s2o3 and 17 mm-kcn. ultraviolet spectrophotometry and thin-layer chromatography showed that the enzyme catalysed the reaction of s2o3(2-) with dihydrolipoic acid or dihydrolipoamide, producing alpha-lipoate or lipoamide, with the intermediate production of the persulphides of dih ...197613142
purification and properties of glutamate synthase from thiobacillus thioparus.glutamate synthase was purified about 250-fold from thiobacillus thioparus and was characterized. the molecular weight was estimated as 280,000 g/mol. the enzyme showed absorption maxima at 280, 380, and 450 nm and was inhibited by atebrin, suggesting that t. thioparus glutamate synthase is a flavoprotein. the enzyme activity was also inhibited by iron chelators and thiolbinding agents. the enzyme was specific for reduced nicotinamide adenine dinucleotide phosphate (nadph) and alpha-ketoglutarat ...197714919
purificantion and characterization of inorganic pyrophosphatase from thiobacillus inorganic pyrophosphatase ec was isolated from thiobacillus thiooxidans and purified 975-fold to a state of apparent homogeneity. the enzyme catalyzed the hydrolysis of inorganic pyrophosphate and no activity was found with a variety of other phosphate esters. the cation mg2+ was required for maximum activity; co2+ and mn2+ supported 25 per cent and 10.6 per cent of the activity with mg2+, respectively. the ph optimum was 8.8. the molecular weight was estimated to be 88,000 by gel fil ...197714950
acidostability of speroplasts prepared from thiobacillus thiooxidans.thiobacillus thiooxidans was acidostable even in the absence of its respiratory substrate, elementary sulfur. this suggests that the acidostability of the bacterium was enery-independent. the organism was subjected to osmotic shock with 0.75 m sucrose at 0 degrees c and then treated with snail intestinal juice in the presence of 0.3 m sucrose. the decrease in the optical density of the sample thus prepared on dilution with deionized water and electron microscopic observation of the sample showed ...197715528
stannous and cuprous ion oxidation by thiobacillus ferrooxidans.oxidation of stannous chloride by thiobacillus ferrooxidans was studied manometrically. at low stannous ion concentrations, initial oxidation rate was proportional to concentration. optimum ph for oxidation was 2.3 optimum temperature was 37-40 degrees c. spectrophotometry showed reduction of cytochromes in suspensions of whole cells on addition of ferrous, stannous, or cuprous salts. cytochrome c reductase activity in cell-free extracts was assayed with ferrous, stannous, or cuprous ions as ele ...197715717
oxidation of stibnite by thiobacillus ferrooxidans.optimum ph, temperature and pulp density for microbiological leaching of museum-grade stibnite mineral has been investigated using a stibnite-adapted strain of thiobacillus ferrooxidans. optimum conditions were found to be ph 1.75, 35 c and 12g solid substrate per 100 ml of basal salts medium as the initial dose. the energy of activation was determined to be 16.8 kcal per mole, and the temperature coefficient 2.2. the highest total dissolved antimony concentration, sbt = sb+3 + sb+5 + i1sbo2+, ...197717364
change of rh2 depending on iron oxidation by thiobacillus ferrooxidans and on mineral acid concentration. 197717952
oxidation kinetics and chemostat growth kinetics of thiobacillus ferrooxidans on tetrathionate and thiosulfate.growth of thiobacillus ferrooxidans in batch culture on 10 mm potassium tetrathionate was optimal at ph 2.5 (specific growth rate, 0.092 h-1). oxygen electrode studies on resting cell suspensions showed that the apparent km for tetrathionate oxidation (0.13 to 8.33 mm) was ph dependent, suggesting higher substrate affinity at higher ph. conversely, oxidation rates were greatest at low ph. high substrate concentrations (7.7 to 77 mm) did not affect maximum oxidation rates at ph 3.0, but produced ...197826665
sulfide oxidation by spheroplasts of thiobacillus ferrooxidans.thiobacillus ferrooxidans is an acidophilic organism important to metal leaching of low-grade ores. the aforementioned importance is related to the ability of the bacterium to oxidize reduced iron and sulfur, principally found in nature as pyrite (fes2). the present study dealt with sulfide oxidation at low ph values and the involvement of the cell envelope in the process of the inorganic oxidations. sulfide oxidation was noted in spheroplasts of t. ferrooxidans prepared by enzymatic and chemica ...197828080
characteristics of thiobacillus thioparus and its thiocyanate assimilation.thiocyanate-assimilatig bacterium, tk 21, was isolated from activated sludge used for the treatment of thiocyanate contained in coke-oven liquor. this organism oxidized thiosulfate and elemental sulfur, causing a decrease of ph of the medium. these facts indicated that it belongs to the genus thiobacillus. potassium thiocyanate (0.5 g/l) was completely assimilated during 60 h. thiosulfate inhibited the assimilation of thiocyanate but elemental sulfur did not. this bacterium did not evolve cyanid ...197828174
oxidation of gallium sulfides by thiobacillus ferrooxidans.the bacterial oxidation of naturally occurring gallium-bearing chalcopyrite concentrate and a pure synthetic gallium (iii) sulfide has been investigated at ph 1.8 and 35 degree c, using an active culture of thiobacillus ferrooxidans. this oxidation process may proceed by direct or by indirect bacterial action. the highest dissolved gallium and copper concentrations were about 2.2 and 40.2 g/l, respectively. the order of the specific rate of oxygen uptake by t. ferrooxidans in approximately cufes ...197828175
epr studies on the blue copper protein, rusticyanin: a protein involved in fe2+ oxidation at ph 2.0 in thiobacillus ferro-oxidans. 197829783
effects of amino acids on thiobacillus acidophilus. i. growth studies with special reference to valine.the heterotrophic growth of thiobacillus acidophilus was inhibited by branched-chain amino acids; valine, isoleucine, and leucine. the inhibition by valine and leucine were partially reversed by isoleucine, and the inhibition by isoleucine was partially reversed by valine. inhibitions by methionine or threonine were partially reversed when both amino acids were present in the growth medium. inhibition by tyrosine was increased by phenylalanine or tryptophan. cystine completely inhibited growth. ...197831972
[effect of ph and temperature on the kinetics of fe2+ oxidation by thiobacillus ferrooxidans].a correlation has been established between the action of ph and temperature on the activity of fe2+ oxidation by thiobacillus ferrooxidans. the rate of fe2+ oxidation by the bacterium is inhibited at 12 degrees c mainly due to an increase in the ph. autooxidation of the medium upon hydrolysis of fe3+ is inhibited at low temperatures. apparently, the enzymes of thiobacillus ferrooxidans involved in fe2+ oxidation are only slightly susceptible to a decrease of temperature to 10--12 degrees c.197934080
transmembrane electrical potential and transmembrane ph gradient in the acidophile thiobacillus ferro-oxidans.thiobacillus ferro-oxidans is capable of using the oxidation of fe2+ by o2 at ph 2.0 as the sole source of energy for growth and co2 fixation. the bacterium maintains an intracellular ph of 6.5 over a range of external ph from 1.0 to 8.0, as measured by [14c]acetate and [3h]methylamine distribution. the membrane potential was estimated by the distribution of the lipid-soluble cation dibenzyldimethylammonium and the anion scn-. at ph 2.0 (the ph of growth) during fe2+ oxidation the transmembrane ...197935160
[relationship of thionic bacteria isolated from geological deposits to the ph of the medium]. 197935734
[mathematical model of thiobacillus ferrooxidans growth on a medium with ferrous iron].a mathematical model is proposed for the growth of thiobacillus ferrooxidans in the process of fe2+ oxidation depending on the active acidity and temperature of the medium. the model was used to establish the correlation between the action of temperature and ph of the medium on the bacterial growth and oxidation of fe2+. the range of ph values which had no effect on the bacterium is broader at 28 degrees c (from 2.5 to 2.8) than at 12 degrees c (from 2.3 to 2.4). apparently, the growth of the ba ...197935735
[characteristics of the proteins of unicellular organisms as potential components of ecological life-support systems].a comparative characterization of the biological value of proteins from green and blue-green algae, bacteria, and microbial coenosis of straw mineralizing active sludge is given with respect to the fractional composition of total protein, its amino acid composition, and affinity for proteolytic enzymes in vitro. the above microorganisms have an adequate amino acid composition, a high content of essential amino acids, and differ in their content of readily soluble proteins. the presence of protei ...197936502
steps of thiosulfate oxidation by thiobacillus thioparus and th. coproliticus.chromatographic analysis was used for studying steps of thiosulfate oxidation by thiobacillus thioparus and th. coproliticus strains, isolated from egyptian soil. th. thioparus strains oxidized s2o3 to so4 with the formation of polythionates, tetrathionate in particular, during the course of oxidation. however, strains differed in other polythionates formed. th. coproliticus, in turn, oxidized s2o3 to so4 without polythionate formation. however, h2s was the only intermediate compound detected du ...197944416
[geomicrobiological studies. xiii. microorganisms and formation of deposits in hot sulfur lakes on hokkaido (japan)]. 197944587
pyrite oxidation by thiobacillus ferrooxidans with special reference to the sulphur moiety of the mineral.available cultures of thiobacillus ferrooxidans were found to be contaminated with bacteria very similar to thiobacillus acidophilus. the experiments described were performed with a homogeneous culture of thiobacillus ferrooxidans. pyrite (fes2) was oxidized by thiobacillus ferrooxidans grown on iron (fe2+), elemental sulphur (s0) or fes2. evidence for the direct utilization of the sulphur moiety of pyrite by thiobacillus ferrooxidans was derived from the following observations: a. known inhibit ...197945294
detection of thiobacillus ferrooxidans in acid mine environments by indirect fluorescent antibody indirect fluorescent antibody (fa) staining technique was developed for the rapid detection of thiobacillus ferrooxidans. the specificity of the fa stain for t. ferrooxidans was demonstrated with both laboratory and environmental samples. coal refuse examined by scanning electron microscopy exhibited a rough, porous surface, which was characteristically covered by water-soluble crystals. significant numbers of t. ferrooxidans were detected in the refuse pores. a positive correlation between n ...197661736
a simple improved method to stain stains are obtained by immersion of the fixed smear in 1.5-2% acid fuchsin at ph 3.2-3.6.197878755
physiology of thermophilic bacteria. 197995242
[sulfobacillus, a new genus of thermophilic sporulating bacteria].an aerobic facultative thermophilic bacterium was isolated from the ore of the nikolaev copper-zinc-pyrite deposit in the eastern kazakhstan. the bacterium is similar to thiobacillus ferooxidans in the ability to use various energy subsrates (fe2+, sulphide minerals and so) and in the acidophilic properties, but differs from it in the thermophilic nature, the structure of a cell wall, the ability to produce spores, and a lower gc content in dna. consequently, the organism has been identified as ...1978101742
ribulose 1,5-bisphosphate carboxylase from autotrophic micro-organisms [proceedings]. 1978105954
[characteristics of sulfur bacteria from lakes of the mari assr].five karst lakes were investigated in the mari autonomous republic. the vertical distribution of thionic bacteria in water was correlated to the distribution and content of dissolved sulphides. the concentration of thionic bacteria was higher in more productive lakes. a symbiotic culture of thionic and denitrifying bacteria, but not thiobacillus denitrificans, was isolated from water of these lakes.1975125841
improved synthesis and rapid isolation millimole quantities of improved enzymatic method for the synthesis of adenylysulfate (aps) from adenosine 5'-phosphate using aps-reductase from thiobacillus denitrificans is described. isolation of millimole quantitities of this sulfur nucleotide is achieved rapidly by means of ion exchange chromatography on a strongly basic ion exchange resin. a facile and reproducible desalting procedure is described.1979156996
distribution of thioredoxins in cyanobacteria.the presence of thioredoxin was demonstrated in 20 strains of cyanobacteria as well as in one phototrophic bacterium rhodopseudomonas sulfidophila and in thiobacillus denitrificans. thioredoxin activity was not found in cyanophora paradoxa and in porphyridium cruentum using the thioredoxin-dependent paps-sulfotransferase activity from synechococcus 6301 as assay system.1979161845
purification and some properties of cytochrome c-552 from a sulfur-oxidizing bacterium, thiobacillus thiooxidans.a soluble cytochrome c-552 from thiobacillus thiooxidans was highly purified and its physico-chemical properteis were studied. the absorption maxima were at 552,523,418 nm in the reduced from and at 412 nm in the oxidized form. the pyridine hemochrome spectrum was the same as that of other cytochromes c. the molecular weight, estimated by the gel filtration method, was found to be 12,600. the isoelectric point was determined to be 9.2-9.3 by the electrofocusing technique. the standard oxidation- ...1975172491
enzymic comparisons of the inorganic sulfur metabolism in autotrophic and heterotrophic thiobacillus ferrooxidans.activities of enzymes which mediate the oxidation of thiosulfate to sulfate and the assimilation of sulfate to sulfide were assayed in various cell-free fractions of thiobacillus ferrooxidans grown autotrophically on either ferrous iron or thiosulfate or heterotrophically on glucose. there was no activity of the thiosulfate-oxidizing enzyme in extracts of bacteria grown with ferrous iron. comparable activities for atp-sulfurylase (ec, adp-sulfurylase (ec, and adenylate kinase ( ...1976175905
d-ribulose-1,5-bisphosphate carboxylase and polyhedral inclusion bodies in thiobacillus intermedius.the growth-related parameters of thiobacillus intermedius, cultured in glutamate-co2-s2o32- medium, have been determined. after centrifugation at 48,000 x g for 1 h, 24% of the d-ribulose-1,5-bisphosphate carboxylase (rubpcase) activity of the disrupted-cell suspensions obtained from co2-s2o32--and glutamate-co2-s2o3(3)- grown cells could be sedimented, and the specific activities of this enzyme in the supernatant fractions were almost equivalent. the enzyme was stable in t. intermedius starved ...1976179979
[oxidation of inorganic compound by autotrophic bacteria (author's transl)]. 1976184217
energy conservation in thiobacillus neapolitanus c6 sulphide and sulphite oxidation. 1977188974
thiobacillus novellus cytochrome oxidase can separate some eucaryotic cytochromes c. 1977193733
a comparison between nitrosomas europaea and thiobacillus novellus on the basis of their oxidation systems of inorganic compounds.nitrosomonas europaea and thiobacillus novellus were compared with each other on the basis of the biochemical properties of their inorganic compound-oxidizing systems. cytochromes c of the two organisms differ considerably from each other; n. europaea cytochrome c-552 belongs to the "bacterial-type" cytochrome c, while t. nouellus cytochrome c-550 resembles eucaryolic cytochrome c. the specificity of cytochrome oxidase for cytochrome c as the electron donor is different between the two organism ...1977198039
on the purification of nitrite reductase from thiobacillus denitrificans and its reaction with nitrite under reducing conditions. 1979220969
subunits of cytochrome a-type terminal oxidases derived from thiobacillus novellus and nitrobacter agilis.cytochrome a-type terminal oxidases derived from thiobacillus novellus and nitrobacter agilis have been purified to a homogeneous state as judged from their electrophoretic behavior and their subunit structures studied by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. the t. novellus enzyme is composed of two kinds of subunits of 32,000 and 23,000 daltons and its minimum molecular weight is 55,000 on the basis of heme content and amino acid composition. the n. agil ...1979229103
[synthesis of d-ribulose-1,5-diphosphate with immobilized enzymes of thiobacillus].preparative synthesis of d-ribulose-1,5-diphosphate (rudp) from ribose-5-phosphate and atp was carried out, using as a catalyst a crude extract of thiobacillus thiooxidans 58 r immobilized on porous glass. the methods for immobiliztion of crude bacterial extracts, synthesis of rudp and purification of the resultant product by means of column chromatography on activated charcoal and anionites were developed. the structure of rudp was identified by 13c-nmr spectroscopy. stability of two phosphate ...1979229481
the soluble adenosine triphosphatase of thiobacillus ferrooxidans.adenosine triphosphatase (atpase) from thiobacillus ferrooxidans was purified 55-fold. polyacrylamide gel electrophoresis of the most purified fraction showed only one major band; histochemical analysis showed that the atpase activity was associated with this band. the ph optimum is 9-10. the enzyme hydrolyzed atp stoichiometrically to adp and inorganic phosphate, the km for this substrate being 7.75 times 10-3 m. gtp and itp are alternate substrates, the km values for these being 6.71 times 10- ...1975234778
thiobacillus acidophilus sp. nov.; isolation and some physiological characteristics.after a brief exposition to glucose, thiobacillus acidophilus was isolated from a culture of iron-grown t. ferrooxidans. physicochemical analysis of its dna showed a g+c content of 62.9-63.2%. the new isolate grows best at 25-30 degrees c and at ph 3.0. growth is possible between ph 1.5 and 6.0. thiobacillus acidophilus is apparently strictly aerobic. ammonium salts are the only suitable source of nitrogen. the bacterium is a facultative autotroph. in addition to elemental sulfur, it obtains ene ...1975234784
physical and chemical studies of thiobacillus ferroxidans lipopolysaccharides.the lipopolysaccharides (lps) of the obligate acidophile thiobacillus ferroxidans grown on iron, sulfur, and glucose as energy sources were examined for various physical and chemical properties. both qualitative and quantitative variation were found among the three preparations. the lps extracted from iron-grown cells (fe-lps) contained less than 3% protein compared to 18 to 25% in lps extracted from either sulfur-grown cells (s-lps) or glucose-grown cells (g-lps). s-lps showed two distinct sedi ...1975238956
phosphoenolpyruvate carboxylase of thiobacillus thioparus. i. general properties.phosphoenolpyruvate (pep) carboxylase (orthophosphate:oxalacetate carboxylase (phosphorylating), ec was purified 19-fold from the obligate chemoautotroph, thiobacillus thioparus. michaelis constants for the substrates were found to be 0.44 mm for phosphoenolpyruvate, 0.89 mm for bicarbonate, and 0.37 mm for magnesium, using tris-hc1, ph 7.3. 1-aspartate, 1-malate, and orthophosphate were found to be inhibitors of enzyme activity, while acetyl coa, fdp, gtp, and cdp had no effect. dioxa ...1975241472
effect of mineral acids on iron bacteria.the oxidative ability of thiobacillus ferroxidans in the presence of different concentrations of h2so4, hc1, hno3, and their mixtures was investigated. bacteriological oxidation of feso4 is expressed as a function of time and acid concentration. for each acid and for their mixtures lethal concentrations for t. ferroxidans were found experimentally. on the basis of the lethal concentration for each acid separately the lethal concentrations of their mixtures were computed and it was found that the ...1975242124
properties and regulation of ribulose diphosphate carboxylase from thiobacillus novellus.ribulose-diphosphate carboxylase from thiobacillus novellus has been purified to hemogeneity as observed by polyacrylamide gel electrophoresis and u.v. light observation during sedimentation velocity analysis. the optimum ph for the enzyme with tris-hcl buffers was about 8.2. concentrations of this buffer in excess of 80 mm were inhibitory. the apparent km for rudp was about 14.8 mum with a hill value of 1.5, for hco3- the apparent km was about 11.7 mm with an n value of 1.18 and for mg2+ about ...1975242294
a rapid method for the determination of cellular protein in the presence of elemental sulfur.cellular protein in the presence of elemental sulfur is determined by the folin reagent after treatment with benzene. using this procedure a generation time of 46.2 +/- 4.2 h was observed for thiobacillus acidophilus grown on elemental sulfur.1977336165
the interpretation of the mass spectrum of an ornithine-containing lipid from thiobacillus thiooxidans.the electron impact mass spectrum of a previously identified ornithine-containing lipid from thiobacillus thiooxidans has been interpreted using exact mass measurements, low and high energy ionization, and defocused metastable studies. the spectrum, which did not contain a molecular ion for the intact lipid, was consistent with cyclization of the ornithine zwitterionic moiety with elimination of water to give 3[3'-(11,12-methylene-2-hydroxyoctadecanoxy)hexadecanylamine]-2-piperidone. production ...1978342005
organic nutrition of chemolithotrophic bacteria. 1978360973
[method of determining the number of thiobacillus thiocyanoxidans in industrial wastes]. 1978364253
bacterial leaching. 1978365458
[physiological properties of the thionic bacteria isolated from paleogene deposits]. 1979375037
[attachment of sulfobacillus thermosulfidooxidans cells to the surface of sulfide minerals].the behaviour of sulfobacillus thermosulfidooxidans cells in cultures growing at 50 degrees c in the autotrophic conditions with intensive stirring of the medium was studied by phase-contrast and electron microscopy. the following compounds (at a concentration of 1%) were used as an energy source: pyrite, chalcopyrite, arsenopyrite, antimonite, galenite, sphalerite, and copper-zinc-pyrite ore. a considerable part of cells was found to be attached to the surface of crystals in the course of oxida ...1979381851
[morphogenetic features of sulfobacillus thermosulfidooxidans].the morphogenesis of sulfobacillus thermosulfidooxidans bkm b-1269 was studied by electron microscopy at the vegetative stage of growth on a medium with pyrite as the source of energy. young growing cultures of this organism were found to be very polymorphous: along with rod-like cells of various dimensions (0.6--0.8x1.0--3.0 mcm) which were predominant, coccoid, pyriform, clavate and dumbbell-like cells were also encountered. certain cells have a tendency for branching. the cells can divide by ...1979388160
rotational mobility of membrane-bound cytochrome o of escherichia coli and cytochrome a1 of thiobacillus ferro-oxidans [proceedings]. 1979389702
[effect of clays on the behavior of acidophilic thiobacillus strains in suspensions]. 1979397686
sulphur in biology. general discussion. 1979398764
[microbial desulfuration of coal. i. isolation and identification of iron- and sulfur-oxidizing bacteria]. 1979400534
[geochemical activity of thionic bacteria]. 1977409914
[ultrastructural organization of sulfobacillus thermosulfidooxidans cells and spores].the ultrastructural organization of sulfobacillus thermosulfidooxidans cells and spores was studied by electron microscopy using the techniques of negative contrasting and thin sections. the cell wall was found to be 20--25 nm thick and to consist of a rigid layer bound to the cytoplasmic membrane with crosspieces. a microcapsule about 20 nm thick having an ordered structure is closely associated with the cell wall. a layer of slime which can reach 0.2--0.3 mcm and more in thickness is located a ...1979481281
[spheroplast study of thiobacillus ferrooxidans].spheroplasts were prepared, under the action of lysozyme (200 and 400 mcg/ml) from the cells of thiobacillus ferrooxidans grown on the waksman medium with elementary sulfur after their treatment with 0.5m nacl solutions and exact exposure to sucrose (0.5m). intracellular structures were revealed on the spheroplasts which could not be detected on intact cells.1979481282
purification of thiobacillus denitrificans siroheme sulfite reductase and investigation of some molecular and catalytic properties.a siroheme-containing sulfite reductase was isolated from thiobacillus denitrificans, purified to an electrophoretically homogenous state, and investigated with regard to some of its molecular and catalytic properties. the enzyme was a tetramer with a molecular weight of 160 000, consisting of two types of subunits arranged to an alpha 2 beta 2-structure. the molecular weight of the alpha-subunit was 38 000, that of the beta-subunit 43 000. as prosthetic groups siroheme and fe/s groupings could ...1979486494
a note on the determination of atp by the luciferin-luciferase bioluminescence reaction in samples containing heavy metals. 1979486773
thermolysis chemical ionization of a complex polar lipid.vaporization of a ornithine-containing polar lipid from thiobacillus thiooxidans has been accomplished by thermolysis in a chemical ionization source. the thermolysis has been shown to be more extensive than previously thought. it occurs in at least two steps, the first being dehydration of the ornithine to produce a substituted piperidone. this fragment undergoes a facile elimination to produce two neutral lipid components: a long chain fatty acid and piperidone-containing fatty amide. the resu ...1979497362
[microbiological leaching of zinc and lead ores of the tekeli deposit].in certain regions of the west tekeli lead-zinc ore deposti, the number of thiobacillus ferrooxidans cells reaches 10(6) per 1 ml of ore water. spontaneous bacterial leaching of sulphide ore is confirmed by an increase in the concentration of ferric iron and zinc in ore water. as a result of this leaching, up to 40 per cent of zinc and 60 per cent of lead have been leached from lead-zinc ore within 271-286 days. bacterial leaching of lead intermediate products has shown that the maximum rate of ...1977600089
[hydrogen sulfide oxidation by thionic bacteria]. 1977600111
bacterial leaching patterns on pyrite crystal surfaces.selected pyrite crystals were placed as a bacterial energy source into stationary cultures of thiobacillus ferroxidans. scanning electron microscope studies performed after a period of 2 years on these crystals revealed bacterial etching pits in characteristic patterns; they include pit arrangements in loose statistical disorder, in pairs, in clusters, and most remarkably in pearl-string-like chains. it has previously been confirmed that the chemical processes of bacterial leaching occur mainly ...1978649567
[thiobacillus ferrooxidans pili].the surface structures of thiobacillus ferrooxidans were studied. when growing on a medium containing elemental sulphur, the cells possess peritrichously located filaments (piles) whose diameter varies from 4.5 to 7.0 nm and length, from 0.7 to 3.0 mcm. the cells of t. ferrooxidans do not have piles on a medium with ferrous iron. the physiological role of these structures for thiobacilli is discussed.1978661638
[dependence of the rate of ferrous oxide oxidation by a thiobacillus ferrooxidans culture on its concentration].the effect of the concentration of ferrous iron on the rate of its oxidation was studied with thiobacillus ferrooxidans. the specific rate of oxidation of ferrous iron was found to increase with its concentration and to decrease with a rise of the total iron content. cultivation of bacteria with electrochemical reduction of fe3+ produced high cell concentrations at a low content of total iron (4--6 g/litre), e. g. upto 4.5 g/litre (dry biomass weight).1978672678
investigations into the kinetics and stoichiometry of bacterial oxidation of covellite (cus) using a polarographic oxygen probe."oxygraph" apparatus was used to measure quantitatively the kinetics of oxidation of synthetic covellite (cus) in the presence and absence of thiobacillus species. the expected stoichiometric relationship between oxygen consumed and cupric sulphate produced was verified by atomic absorption assays of cupric ion and sulphate ion. thiobacillus cultures markedly increased the oxidation rate. the dependence of each oxygen-uptake rate on oxygen concentration was also measured. sterile controls and so ...1978688107
the purification and some properties of rusticyanin, a blue copper protein involved in iron(ii) oxidation from thiobacillus ferro-oxidans.the 'blue' copper-containing protein rusticyanin was purified to homogeneity from cells of the chemolithotrophic bacterium thiobacillus ferro-oxidans by (nh4)so4 fractionation and ion-exchange chromatography. the protein, which is stable at low ph, consists of a single polypeptide chain of mol. wt. 16500 and possesses 0.79 (+/- 0.28)g-atom of cu/mol. the protein, which does not contain arginine residues, has optical absorbance maxima at 287, 450, 597 and 750 nm and is generally similar to azurin ...1978708402
microbial succession and mineral leaching in an artificial coal artificial pyritic coal spoil was prepared and examined over a period of 1.5 years for changes in the population of various physiological varieties of bacteria and also for mineral leaching. heterotrophic bacteria were the first to dominate the spoil, acquiring a population of 10(7) cells per g within 2 weeks. bacteria capable of utilizing choline sulfate as the sole source of energy comprised approximately 1% of the total heterotrophic bacteria. sulfur-oxidizing autotrophic bacteria (thiobac ...1978736542
pyruvate inhibition of the carbon dioxide fixation of the strict chemolithotroph thiobacillus thiooxidans.a flow-through dialysis system used to decrease the concentrations of toxic organic materials excreted by thiobacillus thiooxidans permitted an improved efficiency of carbon dioxide fixation when compared with cells taken from the usual shaken culture. the additions of various concentrations of pyruvic acid and succinic acid inhibited growth significantly. pyruvate at a concentration of 5 x 10(-3) m completely inhibited the respiration of resting cells oxidizing sulfur. the toxicity of pyruvic a ...1975766931
[function of surface membrane structures in thiobacillus thiooxidans].the function of the surface membrane structures was studied with cytochemical techniques on ultrathin sections of thiobacillus thiooxidans. the transport of elementary sulphur inside the cell involves the surface membrane structures, while oxidation of the sulphur to sulphuric acid takes place on the outer surface of the cytoplasmic membrane. the surface membrane structures are supposed also to participate in the primary dissolution of elementary sulphur at the site of contact of the cells with ...1975775257
[degradation of sulfur compounds by pure cultures of thiobacillus]. 1976785827
bacterial leaching of waste uranium materials.the effect of ferrobacteria and thiobacteria on the leaching of waste uranium materials from which 70-80% of uranium was previously leached by classical chemical hydrometallurgical procedure has been investigated. the bacteria used are found in the ore and the mine water of zletovska river locality, yugoslavia. parameters of biological leaching were examined in the laboratory. leaching conditions were changed with the aim of increasing the amount of uranium leached. the effect of pyrite added to ...1976788361
identification of thiobacilli by replica plating on thallous sulfide paper.colonies of thiobacillus thioparus and t. thiooxidans grown on thiosulfate medium can be identified by replica plating on thallous sulfide paper.1976791118
rhodanese from thiobacillus a2: determination of activity by proton nuclear magnetic resonance spectroscopy.rhodanese from thiobacillus a2 was shown by proton nuclear magnetic resonance (nmr) spectroscopy to use dihydrolipoate or dihydrolipoamide as acceptor of the sulphane moiety of thiosulphate with the formation of alpha-lipoate or lipoamide respectively. correlation is shown between assays of the enzyme activity by nmr spectroscopy and by ultraviolet spectrophotometry.1976796411
[comparative biochemistry of dissimilatory nitrite reductases (author's transl)]. 1976818322
purification, quaternary structure, composition, and properties of d-ribulose-1,5-bisphosphate carboxylase from thiobacillus intermedius.d-ribulose-1,5-bisphosphate (rubp) carboxylase has been purified from glutamate-co2-s2o3(2)-grown thiobacillus intermedius by pelleting the enzyme from the high-speed supernatant and by intermediary crystallization followed by sedimentation into a discontinuous 0.2 to 0.8 m sucrose gradient. the enzyme was homogeneous by the criteria of electrophoresis on polyacrylamide gels of several acrylamide concentrations, sedimentation velocity and equilibrium measurements, and electron microscopic observ ...1976819423
bacterial diversity at high temperature. 1976820567
ferric iron reduction by sulfur- and iron-oxidizing bacteria.acidophilic bacteria of the genera thiobacillus and sulfolobus are able to reduce ferric iron when growing on elemental sulfur as an energy source. it has been previously thought that ferric iron serves as a nonbiological oxidant in the formation of acid mine drainage and in the leaching of ores, but these results suggest that bacterial catalysis may play a significant role in the reactivity of ferric iron.1976825043
a study on the reaction mechanism of adenosine 5'-phosphosulfate reductase from thiobacillus thioparus, an iron-sulfur flavoprotein.the reaction mechanism of adenosine 5'-phosphosulfate (aps) reductase (ec from thiobacillus thioparus was studied using difference spectrum and stopped-flow techniques. the enzyme-bound fad was rapidly reduced by sulfite with a first order rate constant of 97.1 s-1. the addition of amp induced further spectral changes in the reduced enzyme which were consistent with the oxidation of fadh2 to the red (anionic) semiquinone fadh-) and the concomitant reduction of nonheme iron to the ferro ...1977837249
leakage of cellular material from thiobacillus ferrooxidans in the presence of organic acids.thiobacillus ferroodixans cells released varying amounts of iron, phosphate, sugar, ribonucleic acid, deoxyribonucleic acid, and substances that absorbed light at both 260 and 280 nm, when exposed to 10(-2) to 10(-1) m concentrations of these organic acids: propionic, butyric, valeric, hexanoic, and oxalacetic. these acids also retarded iron oxidation by the cells. electron microscope observation of cells after exposure to the organic acids showed varying degrees of cell envelope disruption, sug ...1977848961
bacterial oxidation of polythionates: determination of tetrathionate with an ion-selective electrode.a commercially available ion-selective electrode for nitrate was used to continuously monitor tetrathionate oxidation by thiobacillus dentrificans. the electrode was much more sensitive to tetrathionate than to nitrate. the same electrode could also be used for the determination of trithionate.1977848962
glutamate uptake in thiobacillus of the glutamate uptake system of thiobacillus novellus were investigated using intact cells. two kinetically distiguishable components were found and designated at the high-affinity (ha) component with an apparent km of 33 micronm, and the low-affinity (la) component with a km of 18 mm. glutamate accumulation was temperature-sensitive and could be inhibited by the addition of wide range of inhibitors including cyanide, dinitrophenol (dnp), iodoacetate, mercuric chloride, sodium azide ...1977856422
ferrous iron oxidation and uranium extraction by thiobacillus ferrooxidans.the microbiological oxidation of ferrous iron in batch and continuous systems has been investigated in relation to uranium extraction from a low-grade ore by thiobacillus ferrooxidans. the influence of the parameters, agitation, and aeration on oxygen saturation concentration, rate of oxygen mass transfer, and rate of ferrous iron oxidation was demonstrated. the kinetic values, vmax and k were determined using an adapted monod equation for different dilution rates and initial concentrations of f ...1977857953
heterotrophic growth of thiobacillus a2 on sugars and organic acids.thiobacillus a2 grew on a number of organic acids, pentoses, hexoses and alpha-linked disaccharides, but not on beta-linked disaccharides or galactosides. growth was slow on glucose, although fast-growing strains were selectively isolated. additive growth rates occured on glucose and galactose; growth on glucose with fructose, pyruvate or gluconate was biphasic rather than diauxic; fructose was used preferentially over glucose; slow growth on glucose was accelerated by some disaccharides; growt ...1977879963
simultaneous operation of three catabolic pathways in the metabolism of glucose by thiobacillus a2.enzymes essential to the operation of the embden-meyerhof glycolytic pathway, the entner-duodoroff pathway and oxidative pentose phosphate pathway were present in thiobacillus a2 grown on glucose and other sugars. radiorespirometry under various conditions with thiobacillus a2 oxidising glucose specifically labelled with 14c in carbon atoms 1, 2, 3, 3 + 4, 6 or universally labelled demonstrated the simultaneous operation of the embden-meyerhof (48%), entner-doudoroff (28%), and pentose phosphate ...1977879964
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