| d-lactate dehydrogenase of peptostreptococcus elsdenii. | d-lactate dehydrogenase has been purified to near homogeneity from peptostreptococcus elsdenii. as isolated, the enzyme contains flavine adenine dinucleotide and a tightly bound metal cofactor. inactivation by ortho-phenanthroline occurs in two steps and is partially blocked by d-lactate. reactivation by divalent metal ions occurs, with divalent zinc being the most effective. when ferricyanide is used as the electron acceptor, d-lactate has an apparent k0.5 of 3.3 m0.46; its binding is negativel ... | 1975 | 368 |
| purification, new assay, and properties of coenzyme a transferase from peptostreptococcus elsdenii. | coenzyme a (coa) transferase from peptostreptococcus elsdenii has been purified and crystallized, and some of its properties have been established. the work was facilitated by a newly developed coupled and continuous spectrophotometric assay in which the disappearance of added acrylate could be followed at 245 nm. the rate-limiting conversion of acetyl- and beta-hydroxypropionyl coa to acrylyl coa by coa transferase was followed by the non-rate-limiting conversion to beta-hydroxypropionyl coa by ... | 1975 | 369 |
| identification and properties of 8-hydroxyflavin--adenine dinucleotide in electron-transferring flavoprotein from peptostreptococcus elsdenii. | 1. a new flavin prosthetic group has been isolated in pure form from the electron-transferring flavoprotein of peptostreptococcus elsdenni. its structure has been established as the fad derivative of 7-methyl-8-hydroxyisoalloxazine: (see article). proof of this structure has been obtained by chemical syntehsis of 7-methyl-8-hydroxyisoalloxazine models, and by stepwise degradation of the native compound to 7-methy-8-hydroxyalloxazine. the orange chromophore is characterized by a strong absorption ... | 1976 | 4321 |
| non-heme iron proteins. the amino acid sequence of rubredoxin from desulfovibrio vulgaris. | a non-heme iron protein, rubredoxin has been isolated from the sulfate-reducing bacterium, desulfovibrio vulgaris, strain hildenborough. the complete amino acid sequence has been established. the 52 amino acid residues of the protein were aligned with the aid of tryptic and chymotryptic peptides and of a fragment produced by cleavage of the asn-gly bond (22-23) by hydroxylamine. the sequence of the first 30 residues of the molecule was determined using an automatic sequenator, after removal of t ... | 1976 | 7308 |
| studies on the binding of fmn by apoflavodoxin from peptostreptococcus elsdenii, ph and nacl concentration dependence. | 1. the ph and ionic strength dependence of the interaction of fmn with apoflavodoxin has been studied by fluorometry in the ph region 2-5, at 22 degrees c. 2. the rate constant of dissociation and the dissociation constant were experimentally determined; the rate constants of association were claculated at a given ph value. these constants depend on the ionic strength. the plots of these constants against the square root of the ionic strength are straight. 3. our data have been interpreted in te ... | 1976 | 10978 |
| effect of pressure upon the fluorescence of various flavodoxins. | the effects of hydrostatic pressure in the range of 10(-3) to 11 kbar on the fluorescence of flavodoxins from peptostreptococcus elsdenii, desulfovibrio vulgaris, azotobacter vinelandii, and clostridium mp were investigated. the first three flavoproteins showed under high pressure enhancements of flavin fluorescence of over 50 times resulting from the release of flavin mononucleotide from the protein complex. the clostridial flavodoxin showed a very much smaller fluorescence change. at ph 7.5 th ... | 1977 | 20943 |
| a reappraisal of the reaction of butyryl-coenzyme a dehydrogenase with phenylmercuric acetate. evidence that de-greening involves a reaction of the tightly bound thioester. | phenylmercuric acetate reversibly de-greens butyryl-coa dehydrogenase from megasphaera elsdenii, abolishing the absorption band at 710nm. the view that this is a result of modification of a protein thiol group is re-examined in the light of the following new observations. (i) after treatment with phenylmercuric acetate, the enzyme's ability to be re-greened by addition of thiols was not decreased by gel filtration or precipitation with (nh(4))(2)so(4). (ii) phenylmercuric acetate caused the same ... | 1978 | 25661 |
| spontaneous interictal spiking in the awake kindled rat. | the spontaneous interictal spike (sis) noted in eeg recordings is a signal of the abnormally excitable behavior of neurons in an epileptic focus. this study provides a detailed, quantitative, temporal and spatial profile of sis in the amygdaloid kindled rat. the reproducible nature of the development and decline of sis in kindling provides a useful model for biochemical study of the mechanisms that initiate and regulate sis. | 1979 | 91486 |
| the symptomatology with the most severe clinical course of spontaneous subarachnoid hemorrhage. | the symptomatology of 18 patients with the severest clinical course after subarachnoid hemorrhage (sah) is described and analyzed. seventeen patients died, five with an acute bulbar syndrome with cardiac arrest, and 12 with irreversible breakdown of brain function. one patient had an apallic syndrome with minimal signs of remission, who died 4 months after the first rebleeding. the cases are divided into five clinical groups on the basis of the acute brainstem symptomatology which set in instant ... | 1979 | 93630 |
| an unstable donor-recipient dna complex in transformation of bacillus subtilis. | in re-extracted dna obtained shortly after uptake of transforming dna by bacillus subtilis, increased amounts of donor dna radioactivity banding at the position of donor-recipient dna complex (drc) are observed in cscl gradients, if the cells are irradiated with high doses of uv prior to reextraction of the dna. qualitatively, the same phenomenon is observed if lysates of transforming cells are irradiated. uv-irradiation of lysates of competent cells to which single-stranded dna is added after l ... | 1978 | 106232 |
| organization of ground substance proteoglycans in normal and osteoarthritic knee cartilage. | a study of the organization of proteoglycans in articular cartilage indicates that nonaggregated proteoglycans existed in larger numbers in osteoarthritic than in normal cartilage and that proteoglycan aggregates in arthritic cartilage were smaller than normal. after dissociation from hyaluronic acid and tissue glycoproteins, no difference in hydrodynamic size of disaggregated proteoglycans was noted, but chondroitin sulfate chains of those from diseased cartilage were shorter than normal. the d ... | 1976 | 130909 |
| the influence of age on the wound healing of experimental myocardial infarction in rats. | in 6-weeks-old, 5-months-old and 21-months-old rats myocardial infarction was induced by coronary artery ligature. after performing the ligature the animals were administered 3h-thymidine, 3h-proline or 35s-sulphate at different times. the following parameters were determined: number of dna- and tropocollagen-synthesizing connective tissue cells at the infarction border and at the infarction site; mean silver grain density above the nuclei or cells; duration of a cycle; number of mitoses, and in ... | 1975 | 134906 |
| reversible hypothyroidism in growth hormone-deficient children treated with human growth hormone. | six children with human growth hormone (hgh) deficiency became hypothyroid during the course of their therapy with hgh. this was accompanied by a decreasing growth rate, clinical symptoms of hypothyroidism and decreased serum t4 concentrations. three of the 6 patients returned to the euthyroid state, both clinically and biochemically, with cessation of hgh therapy, and reinstitution of hgh precipitated hypothyroidism again in 2 of the three. the patients who remained hypothyroid have evidence of ... | 1975 | 165211 |
| conditional lethal mutants of vesicular stomatitis virus. | | 1975 | 169102 |
| electron-transferring flavoprotein of peptostreptococcus elsdenii that functions in the reduction of acrylyl-coenzyme a. | in peptostreptococcus elsdenii, a three-component flavoprotein electron transfer system catalyzes the oxidation of lactate and the reduction of crotonyl-coenzyme a (coa). spectral evidence showed that d-lactate dehydrogenase, when reduced by d-lactate, was able to reduce butyryl-coa dehydrogenase, but only in the presence of the electron-transferring flavoprotein. reduced nicotinamide adenine dinucleotide could replace reduced d-lactate dehydrogenase. a reconstituted system, containing the three ... | 1975 | 172488 |
| alterations of acetylcholine enzymes in neuroblastoma cells persistently infected with lymphocytic choriomeningitis virus. | | 1977 | 193868 |
| a continuous fluorometric assay for flavokinase. properties of flavokinase from peptostreptococcus elsdenii. | a continuous fluorometric assay that utilizes apoflavodoxin as a trapping agent for riboflavin 5'-phosphate (fmn) has been developed for flavokinase (atp:riboflavin 5'-phosphotransferase, ec 2.7.1.26). use of this assay is illustrated in a procedure for the partial purification of flavokinase from the strict anaerobe peptostreptococcus elsdenii. the purified enzyme catalyzed the formation of 8.3 nmol fmn - min-1 - mg-1 at 37 degrees c and had apparent km values for riboflavin and atp of 10 and 4 ... | 1977 | 195619 |
| [energy metabolism disorders in the myocardium due to alcoholic intoxication]. | energy metabolism was studied in rat myocardial mitochondria by estimation of respiratory enzymes activity and content of the krebs cycle substrates under conditions of acute and chronic intoxication with ethyl alcohol. in the acute intoxication mitochondrial redox enzymes were inhibited (glutamate- and malate dehydrogenases, nadh cytochrome c oxidoreductase and cytochrome c oxidase), succinate- and lactate dehydrogenases were activated; at the same time, contents of pyruvate, succinate, and alp ... | 1977 | 202087 |
| acute encephalopathy with liver dysfunction, chylous ascites and cytomegalovirus infection. | a child with acute encephalopathy and liver dysfunction subsequently developed acute chylous ascites. titers for cytomegalovirus increased from less than 1:2 to 1:32 during the illness, and cytomegalovirus was isolated from the urine. the case is the first one possibly linking cytomegalovirus and acute encephalopathy and liver dysfunction in a child. in our patient, enlargement of the abdominal lymph nodes, as seen on a lymphangiogram, resulted in a severe obstruction of abdominal lymphatic flow ... | 1977 | 202566 |
| noradrenaline receptor sensitivity after chronic ethanol administration. | the effects of the central noradrenaline receptor stimulating agent clonidine on blood pressure, heart rate, and flexor reflex activity were studied in intact rats and in rats chronically treated with ethanol. the clonidine-induced changes were similar in ethanol-treated animals and in animals never subjected to ethanol. the results suggest that the sensitivity of central noradrenaline receptors involved in the mediation of the circulatory changes and of the flexor reflex activity after clonidin ... | 1978 | 211201 |
| escherichia coli mutants defective in dipeptidyl carboxypeptidase. | two independent mutants of escherichia coli deficient in dipeptidyl carboxypeptidase activity (dep-) were isolated after mutagenesis with ethyl methanesulfonate. mating experiments and introduction of specific episomes indicated that the responsible gene was located at approximately 27--31 min on the e. coli chromosome. the dep- mutants differed from the parental strain in their inability to grow with n-acetylalanylalanylalanine as the sole nitrogen source. revertants selected for growth on this ... | 1978 | 216006 |
| enhancement of alcohol withdrawal seizures with 6-hydroxydopamine. | the relationship between central catecholamine neurons and alcohol withdrawal seizures was studied in rats pretreated with 6-hydroxydopamine to selectively destroy the central catecholamine nerve terminals. the alteration of alcohol withdrawal seizures was manifested by (1) a higher percentage of the rats developing the seizures, (2) an earlier onset of seizures, and (3) a greater severity of seizure activity. seizure status was exhibited in 25% of the 6-hydroxydopamine-treated rats. | 1978 | 216544 |
| activation of histidine decarboxylase by h2-receptor blockade: mechanism of action. | 1 treatment with histamine h2-receptor antagonists, which inhibit basal acid secretion was found to activate rat stomach histidine decarboxylase. at the same time the serum gastrin concentration was greatly increased. 2 in antrectomized rats neither the enzyme activity nor the serum gastrin concentration was affected by the treatment. 3 in analogy with previous observations on other inhibitors of acid secretion we suggest that the h2-receptor antagonists stimulate gastrin release through their e ... | 1975 | 236059 |
| influence of histamine h1- and h2-receptor blockers on sympathetic vasodilator and vasoconstrictor responses in canine paw. | 1 vasodilator responses to histamine, bradykinin and sympathetic nerve stimulation were elicited in the perfused paw of dogs treated with bretylium (15-20 mg/kg) and atropine. the h2-receptor blocking agent, burimamide, when administered in the dose of 5 mg/kg intravenously and 4 mg intra-arterially did not depress significantly these vasodilator responses. the subsequent administration of tripelennamine in the dose of 2.5-5 mg/kg intravenously and 4 mg intra-arterially produced a significant bl ... | 1975 | 236060 |
| d-lactate dehydrogenase of peptostreptococcus elsdenii. | | 1975 | 236453 |
| binding of digitoxin to human serum proteins: influence of ph on the binding of digitoxin to human albumin. | | 1975 | 238355 |
| suppression of phytohemagglutinin response by fungi from a "leukemia" house. | fungal isolates from the house of a husband and wife who both developed acute myelomonocytic leukemia were assayed for effects on the in vivo response to phytohemagglutinin in guinea pigs. skin responses to intradermal phytohemagglutinin were measured following injections of sterile fungal extracts. isolates of penicillium canescens, curvularia, fusarium sambucinum, fusarium equiseti and trichoderma koningii from the leukemia-associated house depressed the responses to phytohemagglutinin, but no ... | 1979 | 291385 |
| characterization of endotoxin from the rumen bacterium megasphaera elsdenii. | phenol-water extraction of megasphaera elsdenii, a predominant gram-negative coccus in rumens of cattle fed high-grain diets, yielded material that exhibited typical characteristics of endotoxin. the extract was lethal to mice and to chicken embryos, caused biphasic fever in rabbits, leukopenia in mice, and local and generalized shwartzman reactions; and induced tolerance to the lethal effect of the endotoxin in mice. the material contained carbohydrate, protein, lipid, phosphorus, and 2-keto-3- ... | 1979 | 453682 |
| purification and properties of the flavoenzyme d-lactate dehydrogenase from megasphaera elsdenii. | a pyridine nucleotide independent d-lactate dehydrogenase has been purified to apparent homogeneity from the anaerobic bacterium megasphaera elsdenii. the enzyme has a molecular weight of 105 000 by sedimentation equilibrium analysis with a subunit molecular weight of 55 000 by sodium dodecyl sulfate gel electrophoresis and is thus probably a dimer of identical subunits. it contains approximately 1 mol of fad and 1 g-atom of zn2+ per mol of protein subunit, and the flavin exhibits a fluorescence ... | 1979 | 497162 |
| suicide inactivation of the flavoenzyme d-lactate dehydrogenase by alpha-hydroxybutynoate. | the acetylenic alpha-hydroxy acid 2-hydroxy-3-butynoate (alpha hb) is a substrate and an irreversible inactivator of the fad-containing flavoenzyme d-lactate dehydrogenase from megasphaera elsdenii. on the average, the enzyme undergoes five catalytic turnovers with alpha hb in air at ph 7.0 before being inactivated. irreversible inactivation is due to the conversion of the flavin to a pink adduct with visible absorption peaks at 522, 382, and 330 nm and weak fluorescence with an emission maximum ... | 1979 | 497163 |
| structure of the flavin adduct formed in the suicide reaction of alpha-hydroxybutynoate with d-lactate dehydrogenase. | the zn-dependent flavoenzyme d-lactate dehydrogenase from megasphaera elsdenii is irreversibly inactivated by the d form of the suicide substrate 2-hydroxy-3-butynoic acid. the process of inactivation involves formation of a new pink chromophore, which can be released in intact form from the protein and which was purified to homogeneity by affinity chromatography. inactivation involves covalent addition of the suicide substrate to the flavin coenzyme. the optical spectra indicate an elongation o ... | 1979 | 497164 |
| megasphaera elsdenii endocarditis. | a case of endocarditis caused by megasphaera elsdenii is reported. this anaerobic grim-negative coccus has rarely been associated with human infections and has not previously been described as a cause of endocarditis. | 1979 | 500797 |
| purification and properties of hydrogenase from megasphaera elsdenii. | a hydrogenase has been purified to homogeneity from the soluble fraction of the rumen bacterium megasphaera elsdenii, the overall purification is 200 times with a yield of 14%. the pure enzyme consists of a single polypeptide chain with mr approximately 50 000 which contains 12 atoms of non-haem iron and 12 atoms of acid-labile sulphide. the enzyme is rapidly inactivated by o2 and it is therefore purified under nitrogen and in the presence of sodium dithionite. the optical spectrum of the enzyme ... | 1979 | 527582 |
| transformation of mercuric chloride and methylmercury by the rumen microflora. | the microflora in strained rumen fluid did not methylate or volatilize 203hg2+ at detectable rates. however, there was an exponential decay in the concentration of added ch3hg+, which was attributed to demethylation. the major product of demethylation was metallic mercury (hg0), and it was released as a volatile product from the reaction mixture. demethylation occurred under both anaerobic and aerobic conditions. the rate of demethylation was proportional to the concentration of added ch3hg+-hg ... | 1979 | 539820 |
| comparison of three methods of susceptibility testing of bacteroidaceae, peptococcaceae and other anaerobes to doxycycline. | the in vitro activity of doxycycline against 147 strains of gram-positive and gram-negative anaerobes was determined by broth dilution, agar dilution and agar diffusion tests. the strains were isolated from clinical specimens in 1977. saccharolytic bacteroides strains (39 b. fragilis, 6 b. thetaiotaomicron, 4 b. vulgatus) showed broth dilution mics of less than or equal to 0.0625-4.0 microgram/ml after 5 h incubation and mics of less than or equal to 16 microgram/ml after 15 h incubation at 37 d ... | 1979 | 543353 |
| studies on some characteristics of hydrogen production by cell-free extracts of rumen anaerobic bacteria. | hydrogen production was studied in the following rumen anaerobes: bacteroides clostridiiformis, butyrivibrio fibrisolvens, enbacterium limosum, fusobacterium necrophorum, megasphaera elsdenii, ruminococcus albus, and ruminococcus flavefaciens. clostridium pasteurianum and escherichia coli were included for comparative purposes. hydrogen production from dithionite, dithionite-reduced methyl viologen, pyruvate, and formate was determined. all species tested produced hydrogen from dithionite-reduce ... | 1977 | 558042 |
| production of branched-chain volatile fatty acids by certain anaerobic bacteria. | net production of isobutyric acid, isovaleric acid, and 2-methylbutyric acid by cultures of bacteroides ruminicola and megasphaera elsdenii on media that contained trypticase or casein hydrolysate continued (up to 5 days) after growth had ceased. only trace quantities of these acids were produced in a medium that contained a mixture of amino acids that did not include the branched-chain amino acids. m. elsdenii produced increased quantities of the branched-chain fatty acids in a medium that cont ... | 1978 | 566082 |
| nutritional characteristics of megasphaera elsdenii. | megasphaera elsdenii b159 (formerly peptostreptococcus elsdenii; rogosa 1971) is able to grow in a chemically defined medium containing minerals, nh4+ as the sole nitrogen source, sulfate as the source of sulfur, acetate and glucose as the carbon and energy sources, and the vitamins biotin, pyridoxine, and calcium pantothenate. when lactate serves as the carbon source, with cysteine present as the reducing agent, acetate is not required. valine and threonine appeared to be required although dire ... | 1978 | 688105 |
| some effects of arsenic on the rumen microflora; an in vitro study. | the rate of fermentation of the rumen microflora was inhibited almost 30% by 5 micrograms/ml of arsenic added in the form of arsenite, although 304 micrograms/ml was required to cause 50% inhibition. arsenate was less inhibitory. the rate of fermentation of a separated bacterial fraction was inhibited 37% by 1 microgram of arsenite per millilitre, whereas 100 micrograms/ml had little effect on the fermentation of a separated protozoal fraction. similar results were obtained for arsenate. both fr ... | 1978 | 754875 |
| phylogenetic studies of two rubredoxins from sulfate reducing bacteria. | the sequences of two rubredoxins isolated from the sulfate reducing bacteria: desulfovibrio vulgaris and desulfovibrio gigas have been elucidated. they have similar sequences but many more differences occur than would be expected from two bacteria of the same genus. of the 52 sites, only 37 are occupied by identical residues. the primary structures are compared with those of the anaerobic bacteria rubredoxins of clostridium pasteurianum, micrococcus aerogenes, pseudomonas oleovorans and peptostr ... | 1977 | 864718 |
| effect of ruminal lactic acid-utilizing bacteria on adaptation of cattle to high-energy rations. | heifers, unadapted to a concentrate ration, were intraruminally inoculated (1 dose) with cultures of ruminal lactic acid-utilizing bacteria or with ruminal fluid from a steer adapted to a concentrate ration. inoculation with cultures (1 l) of selenomonas ruminantium or megasphaera elsdenii did not produce better average daily weight gains or feed efficiency of heifers fed a high-energy ration for 21 days, if these values were compared with the performance of noninoculated heifers. average daily ... | 1977 | 883707 |
| kinetics and catalytic properties of coenzyme a transferase from peptostreptococcus elsdenii. | coenzyme a (coa) transferase from peptostreptococcus elsdenii was purified to homogeneity, and some of its physical and catalytic properties were determined. the native enzyme has a molecular weight of 181,000 and is composed of two alpha subunits (molecular weight, 65,000) and one beta subunit (molecular weight 50,000). heat treatment of the crude cell extract to 58 degrees c causes proteolysis of the native enzyme and yields a catalytically active enzyme with an approximate molecular weight of ... | 1976 | 977540 |
| 4-alkyldihydroflavin: coenzyme synthesis and modification of flavodoxin. | a stable (i.e. non-oxidisable), reduced flavocoenzyme, 4a-(n-propyl)-4a,5-dihydro-riboflavin 5'-monophosphate, has been synthesized. the method of choice was selective photo-allylation in position 4a. stabilization of this substituent was achieved by hydrogenation after n(5) protection, while hydrogenation of the unprotected compound would have led to removal of the allyl substituent. the mode of this substituent mobility has been checked with deuterated photosubstrate. upon hydrogenation, the a ... | 1976 | 991861 |
| occurrence of megasphaera elsdenii in faecal samples of young pigs. | important numbers of megasphaera elsdenii, known as a normal inhabitant of the rumen of cattle and sheep were recently cultured from intestinal contents of man and pigs. studying the occurrence of this organism in faecal contents of pigs, we paid attention to the use of a strict anaerobic isolating and counting technique. it was demonstrated that megasphaera elsdenii is a strict anaerobic organism. exposure to air during 15 minutes caused an important decrease of the colony count. comparison of ... | 1975 | 1098324 |
| transfer of toxin susceptibility to plant protoplasts via the hemlmintosporoside binding protein of sugarcane. | | 1975 | 1169066 |
| lipid-phase transitions of the strictly anaerobic bacteria veillonella parvula and anaerovibrio lipolytica. | as a basis for physicochemical studies on the membranes of the strictly anaerobic bacteria veillonella parvula, anaerovibrio lipolytica, and megasphaera elsdenii, the fatty acyl and alk-1-enyl moieties on the phosphoglycerides of these organism were characterized. uncommon is the high proportion of a heptadecenoic acyl and alk-1-enyl moiety in these three lactate-fermenting bacteria. in contrast to v. parvula and a. lipolytica, m. elsdenii contains high amounts of branched-chain acyl and alk-1-e ... | 1975 | 1194242 |
| characterization of a reserve glucan from megasphaera elsdenii. | a reserve glucan of megasphaera elsdenii was studied by methylation analysis before and after treatment with isoamylase. the results of this study indicate that the glucan is of the amylopectin-glycogen type. | 1975 | 1201512 |
| properties of immobilized flavodoxin from peptostreptococcus elsdenii. an affinity ligand for the purification of riboflavin 5'-phosphate (fmn) and its analogues. | the small flavoprotein, flavodoxin, isolated from peptostreptococcus elsdenii, has been covalently coupled to cnbr-activated sepharose 4b. the immobilized protein replaces ferredoxin as an electron carrier in hydrogen production from dithionite or pyruvate in the presence of ferredoxin-free extracts of p. elsdenii; compared with soluble flavodoxin, its activities in these systems are 13% and 3.5% respectively. acid treatment reversibly dissociates fmn from the immobilized protein. the dissociati ... | 1975 | 1204624 |
| lactic acid-utilizing bacteria in ruminal fluid of a steer adapted from hay feeding to a high-grain ration. | a mature, rumen-cannulated steer fed coastal bermuda grass hay for 8 weeks was adapted to a high-grain ration by stepwise increases in grain over a period of 4 weeks. the grain rations had concentrate-to-roughage ratios of 40:60, 70:30, and 85:15 and were fed for 10, 7, and 11 days, respectively. numerical estimates of lactate-utilizing bacteria in ruminal fluid of the steer the last 3 days each ration was fed were made by colony counts. lactate-utilizing bacteria were identified as megasphaera ... | 1976 | 1275348 |
| [differential diagnosis of ascites and abscess forming hepatitis in aids patients with reference to the first patient with microsporidia infection in austria]. | we report on a 30 years-old female aids patient suffering from generalized pneumocystosis and intestinal microsporidiosis. the chest x-ray showed a right-sided pleural effusion; the lungs showed no areas of consolidation and the heart and the vessels were normal in size. sonography revealed multiple cystic lesions of the liver measuring 1-3 cm in diameter, as well as ascites. pneumocystis carinii was detected on cytological examination of the bronchoalveolar lavage fluid, the pleural effusion an ... | 1992 | 1288019 |
| reproductive and teratogenic effects of a major earthquake in rats. | | 1992 | 1318458 |
| the ph in reversed micelles as imposed by the dihydrogen/proton redox couple and indicated by viologens and cytochrome c3 using hydrogenase as redox catalyst. | the ph values in reversed micelles were measured, making use of the hydrogenase enzyme as redox catalyst short-circuiting the viologen oxidized/semiquinone redox states. the hydrogenases from desulfovibrio vulgaris (hildenborough) and from megasphaera elsdenii were applied. the observed ph values in reversed micelles were not dependent on the type of hydrogenase. two cationic [cetyltrimethylammonium bromide and dodecylammonium propionate (dap)] and two anionic sodiumdodecyl sulphate, sodium di(e ... | 1992 | 1321716 |
| 13c nmr of cyanylated flavodoxin from megasphaera elsdenii and of thiocyanate model compounds. | both of the thiol groups of megasphaera elsdenii flavodoxin have been cyanylated using 13c-enriched cyanide. this chemical modification increases the dissociation constant of the apoflavodoxin-flavin mononucleotide (fmn) complex from 0.4 nm to 2 microm. the thiocyanate carbons of the cyanylated cysteine residues in apoflavodoxin had 13c chemical shifts of 109.4 ppm and 112.2 ppm, which were replaced by signals at 115.5 ppm and 109.6 ppm when fmn was bound. the signals at 109.4 ppm and 112.2 ppm ... | 1992 | 1510978 |
| the hydrogen-tritium exchange activity of megasphaera elsdenii hydrogenase. | the hydrogenase of megasphaera elsdenii was purified to a specific activity of 350 units/mg. the hydrogen-tritium exchange assay of hallahan et al. [hallahan, d.l., fernandez, v. m., hatchikian, e. c. and hall, d. o. (1986) biochimie (paris) 68, 49-54] was adapted to allow its use in the study of the m. elsdenii hydrogenase preparation. under the assay conditions routinely employed, the enzyme's exchange activity was inhibited by tris/hcl and mgcl2; it was stimulated by ethylene glycol. maximal ... | 1992 | 1555573 |
| susceptibilities of anaerobic bacteria isolated from animals with ovine foot rot to 28 antimicrobial agents. | the agar dilution method was used to determine the inhibitory activities of 28 antimicrobial agents against 35 strains of the genus peptostreptococcus, 4 strains of the species peptococcus niger, 20 strains of the species megasphaera elsdenii, 7 strains from the species acidaminococcus fermentans, 8 strains of the genus clostridium, 11 strains of the genus eubacterium, and 1 strain of the species propionibacterium acidipropionici, all of which were isolated from 125 clinical cases of ovine foot ... | 1992 | 1590689 |
| glutamate neurotoxicity is independent of calpain i inhibition in primary cultures of cerebellar granule cells. | glutamate-induced neurotoxicity and calpain activity were studied in primary cultures of rat cerebellar granule neurons and glial cells. calpain activation, as monitored by quantitative immunoblotting of spectrin, required micromolar concentrations of ca2+ in neuronal homogenates (calpain i) and millimolar ca2+ concentrations in glial homogenates (calpain ii). glutamate-induced toxicity and calpain activation were observed in neuronal, but not in glial, cultures. in neurons, calpain i activation ... | 1991 | 1680162 |
| a survey of peptidase activity in rumen bacteria. | twenty-nine strains of 14 species of rumen bacteria were screened for their ability to hydrolyse ala2, ala5, glyarg-4-methoxy-2-naphthylamide (glyarg-mna) and leu-mna. several species, notably megasphaera elsdenii, were active against ala2, and a smaller number, including bacteroides ruminicola, butyrivibrio fibrisolvens, ruminococcus flavefaciens, lachnospira multipara and ruminobacter amylophilus, broke down ala5. streptococcus bovis had an exceptionally high leucine arylamidase activity. howe ... | 1991 | 1748877 |
| intracellular ph of acid-tolerant ruminal bacteria. | acid-tolerant ruminal bacteria (bacteroides ruminicola b1(4), selenomonas ruminantium hd4, streptococcus bovis jb1, megasphaera elsdenii b159, and strain f) allowed their intracellular ph to decline as a function of extracellular ph and did not generate a large ph gradient across the cell membrane until the extracellular ph was low (less than 5.2). this decline in intracellular ph prevented an accumulation of volatile fatty acid anions inside the cells. | 1991 | 1781695 |
| three-dimensional correlated nmr study of megasphaera elsdenii flavodoxin in the oxidized state. | the value of a three-dimensional (3d) non-selective total correlation/nuclear overhauser enhancement spectroscopy (tocsy-noesy) spectrum for making sequential resonance assignments in proteins is demonstrated using the relatively large megasphaera elsdenii flavodoxin (molecular mass 15 kda) in the oxidized state. an easy and concise method for the analysis of 3d-nmr spectra and a strategy for the resonance assignment of 3d-nmr protein spectra is introduced. in this context, non-selective tocsy-n ... | 1991 | 1999199 |
| secondary and tertiary structure characteristics of megasphaera elsdenii flavodoxin in the reduced state as determined by two-dimensional 1h nmr. | the secondary structure of two-electron-reduced megasphaera elsdenii flavodoxin has been determined by visual, qualitative inspection of the sequential connectivities involving c alpha h, c beta h and nh protons observed in noesy (two-dimensional nuclear overhauser enhancement spectroscopy) spectra. results from an amide proton exchange experiment were used to confirm the secondary structure assignment and to demonstrate the compactness and stability of the protein. after the secondary structure ... | 1990 | 2161759 |
| [new antitubercular drugs]. | | 1990 | 2168041 |
| the structure and mechanism of iron-hydrogenases. | hydrogenases devoid of nickel and containing only fe-s clusters have been found so far only in some strictly anaerobic bacteria. four fe-hydrogenases have been characterized: from megasphaera elsdenii, desulfovibrio vulgaris (strain hildenborough), and two from clostridium pasteurianum. all contain two or more [4fe-4s]1+,2+ or f clusters and a unique type of fe-s center termed the h cluster. the h cluster appears to be remarkably similar in all the hydrogenases, and is proposed as the site of h2 ... | 1990 | 2173950 |
| physical studies on the membranes and lipids of plasmalogen-deficient megasphaera elsdenii. | membrane fluidity and thermotropic phase behavior in the wild-type and plasmalogen-deficient strains of megasphaera elsdenii have been studied by means of diphenylhexatiene steady state fluorescence anisotropy in isolated membranes, and by 31p-nmr and x-ray diffraction of the isolated phospholipids. compared to the wild-type plasmalogen content of greater than 75%, plasmalogen-deficient strains had less than 5% plasmalogen, consisting largely of phosphatidylethanolamine and phosphatidylserine. s ... | 1990 | 2208444 |
| radioimmunotargeting of human tumour cells in immunocompetent animals. | a tumour model system is reported that for many purposes may be an alternative to xenografted nude mice. the model allows immunotargeting of human tumour cells in immunocompetent animals. the target cells are contained in i.p. diffusion chambers (dc) with micropore membrane walls that are permeable to molecules, including the cell specific monoclonal antibodies (moab), but impermeable to cells. thus, the tumour cells are protected from the host immunocompetent cells. in the work here presented t ... | 1990 | 2223574 |
| radioimmunotargeting of human tumour cells in immunocompetent animals. | a tumour model system is reported that for many purposes may be an alternative to xenografted nude mice. the model allows immunotargeting of human tumour cells in immunocompetent animals. the target cells are contained in i.p. diffusion chambers (dc) with micropore membrane walls that are permeable to molecules, including the cell specific monoclonal antibodies (moab), but impermeable to cells. thus, the tumour cells are protected from the host immunocompetent cells. in the work here presented t ... | 1990 | 2223574 |
| isolation and identification of anaerobic bacteria from ovine foot rot in spain. | a microbiological study was made of 125 merino sheep showing clinical signs of foot rot. a total of 435 strictly anaerobic strains were isolated, belonging to the following genera: bacteroides, peptostreptococcus, tissierella, fusobacterium, megasphaera, eubacterium, acidaminococcus, clostridium, peptococcus and propionibacterium. of the 35 species obtained, the following were found in more than 10 per cent of animals sampled: bacteroides nodosus, b putredinis, b buccae, b ruminicola subspecies ... | 1990 | 2236925 |
| tertiary structure of two-electron reduced megasphaera elsdenii flavodoxin and some implications, as determined by two-dimensional 1h-nmr and restrained molecular dynamics. | the tertiary structure of the non-crystallizable two-electron-reduced megasphaera elsdenii flavodoxin (15 kda, 137 amino acid residues) has been determined using nuclear overhauser enhancement restraints extracted from two-dimensional 1h-nmr spectra. a tertiary structure satisfying the experimental restraints very well (maximum noe violation of 66 pm) was obtained with use of restrained molecular dynamics, using 509 distance restraints (including one non-noe) on a starting structure modeled from ... | 1990 | 2253614 |
| a two-dimensional 1h-nmr study on megasphaera elsdenii flavodoxin in the oxidized state and some comparisons with the two-electron-reduced state. | assignments for the 137 amino acid residues of oxidized megasphaera elsdenii flavodoxin have been made using the sequential resonance assignment procedure. great benefit was experienced from assignments of the fully reduced protein. the secondary and tertiary structures of the typical alpha/beta protein remain virtually identical on going from the oxidized to the two-electron-reduced state as judged from two-dimensional noe spectroscopy. however, functionally important conformation changes in th ... | 1990 | 2253616 |
| structure and oxidation-reduction behavior of 1-deaza-fmn flavodoxins: modulation of redox potentials in flavodoxins. | flavodoxins from clostridium beijerinckii and from megasphaera elsdenii with 1-carba-1-deaza-fmn substituted for fmn have been used to study flavin-protein interactions in flavodoxins. the oxidized 1-deaza analogue of fmn binds to apoflavodoxins from m. elsdenii and c. beijerinckii (a.k.a. clostridium mp) with association constants (ka) of 1.0 x 10(7) m-1 and 3.1 x 10(6) m-1, values about 10(2) less than the corresponding ka values for fmn. x-ray structure analysis of oxidized 1-deaza-fmn flavod ... | 1990 | 2261478 |
| obligately anaerobic bacterial species isolated from foot-rot lesions in goats. | lesions showing clinical signs of foot-rot from 120 goats were cultured on six selective media during october 1987 to november 1988. a total of 582 strictly anaerobic microorganisms belonging to 50 different species was isolated and identified. the anaerobes most frequently isolated belonged to the following genera: bacteroides (80%), peptostreptococcus (63.6%), megasphaera (40%), fusobacterium (29.2%), clostridium (22.5%), propionibacterium (12.5%), eubacterium (11.7%) and leptotrichia (10.8%). ... | 1990 | 2271912 |
| a two-dimensional 1h nmr study on megasphaera elsdenii flavodoxin in the reduced state. sequential assignments. | assignments for the 137 amino acid residues of megasphaera elsdenii flavodoxin in the reduced state have been made using the sequential resonance assignment procedure. several hydroxyl and sulfhydryl protons were observed at 41 degrees c at ph 8.3. spin systems were sequentially assigned using phase-sensitive two-dimensional-correlated spectroscopy and phase-sensitive nuclear overhauser enhancement spectroscopy. spectra of the protein in h2o and of protein preparations either completely or partl ... | 1990 | 2303055 |
| spectral and electrochemical properties of glutaryl-coa dehydrogenase from paracoccus denitrificans. | studies of the spectral (uv/vis and resonance raman) and electrochemical properties of the fad-containing enzyme glutaryl-coa dehydrogenase (gcd) from paracoccus denitrificans reveal that the properties of the oxidized enzyme (gcdox) appear to be invariant from those properties known for other acyl-coa dehydrogenases such as mammalian general acyl-coa dehydrogenase (gacd) and butyryl-coa dehydrogenase (bcd) from megasphaera elsdenii. however, when either free or complexed gcd is reduced, its spe ... | 1990 | 2340266 |
| direct electron transfer of redox proteins at the bare glassy carbon electrode. | a simple system is presented for the microscale, direct voltammetry of redox proteins, typically 25 micrograms, in the absence of mediators and/or modifiers. the sample consists of a droplet of anaerobic solution laid onto an oversized disc of nitric-acid-pretreated glassy carbon as the working electrode. very reproducible, nernstian responses are obtained with horse heart cytochrome c. the midpoint potential em (ph 7.0) is dependent on the ionic strength, ranging from $293 mv in 1 mm potassium ... | 1989 | 2546759 |
| hydrodynamic, structural and magnetic properties of megasphaera elsdenii fe hydrogenase reinvestigated. | megasphaera elsdenii hydrogenase has been purified to homogeneity using an fplc procedure as the final step. the protein gives a single band in sds/page with an apparent molecular mass of 57-59 kda. there is no second hydrogenase activity in the soluble fraction of m. elsdenii. the hydrodynamics of the enzyme have been compared to those of the two-subunit fe hydrogenase from desulfovibrio vulgaris (hildenborough) in the analytical ultracentrifuge using the absorption of the intrinsic iron-sulfur ... | 1989 | 2556270 |
| identification of the dna damage-responsive element of rnr2 and evidence that four distinct cellular factors bind it. | the rnr2 gene encodes the small subunit of ribonucleotide reductase, the enzyme that catalyzes the first step in the pathway for the production of the deoxyribonucleotides needed for dna synthesis. transcription of this gene is induced approximately 20-fold in response to environmental stimuli that damage dna or block dna replication. deletion and subcloning analysis identified two, and possibly three, upstream activating sequences (uas) and one repressing (urs) element in the rnr2 regulatory re ... | 1989 | 2685561 |
| metabolism and some characteristics of ruminal strains of megasphaera elsdenii. | megasphaera elsdenii belongs to the group comprising the ruminal and intestinal lactate- and sugar-fermenting species. in the present study the fermentation characteristics, metabolism of glucose and lactate, and susceptibility to antimicrobial agents of four ruminal strains were investigated. particular attention was given to the mixed-substrate fermentation pattern and resultant fermentation acid profile. lactate was utilized more rapidly than glucose in media with both carbon sources. interac ... | 1989 | 2764566 |
| [bacteriological study of chronic paranasal sinusitis]. | analysis was done on the bacteria flora and the antibiotic sensitivity of 533 maxillary sinuses in 430 patients with chronic sinusitis treated at the kaohsiung medical college hospital during the past 10 years. among antral flora, streptococcus sp. were the most common (212 strains, 38.4%), followed by staphylococcus epidermidis (140 strains, 25.4%) and staphylococcus aureus (33 strains, 5.9%). these three g(+) bacteria made up 70% (69.87%) of the total. the percentage of gram-negative and anaer ... | 1989 | 2778857 |
| the dilation of arterioles in frog submaxillaris muscle caused by visible light. | transillumination of submaxillaris muscle with incandenscent lamp led to arteriolar dilation if the illuminance exceeded 1-2 x 10(3) lux or reached the time-mean value of 3 x 10(4) lux when a flash lamp was used (pulse duration 10 microseconds, repetition rate 50 s-1). the temperature increase within a light spot on the muscle did not exceed 0.1 degrees c, visible light being the only reason of the dilation of arterioles. at 6.3 x 10(4) lux (incandescent lamp, green filter) the latency of dilati ... | 1989 | 2786511 |
| ontogeny of androgen receptors in fetal guinea pig brain. | sexual differentiation of the guinea pig brain is androgen dependent. to understand the cellular mechanisms of androgen action, we studied the ontogeny of cytosolic (arc) and nuclear (arn) androgen receptors in the brains and anterior pituitaries of fetal, neonatal, and adult guinea pigs. using cytosol from the hypothalamus-preoptic area-amygdala-septum of 60- to 65-day fetuses and nuclear preparations from 6-day-old neonates treated with testosterone propionate, validation studies revealed an a ... | 1989 | 2804213 |
| a study of one of the iron-sulphur clusters in oxidized hydrogenase from megasphaera elsdenii by magnetic-circular-dichroism spectroscopy. | the m.c.d. spectrum of the oxidized state of hydrogenase from megasphaera elsdenii has been measured at liquid-helium temperatures. this oxidation state of the enzyme displays a characteristic rhombic e.p.r. signal with g-values of 2.101, 2.052 and 2.005 assigned previously to a [4fe-4s]3+ cluster as in oxidized hipip (high-potential iron-sulphur protein) [van dijk, grande, mayhew & veeger (1980) eur. j. biochem. 107, 251-261]. the low-temperature m.c.d. spectrum shows no features attributable t ... | 1985 | 2986607 |
| on the novel h2-activating iron-sulfur center of the "fe-only" hydrogenases. | the two hydrogenases (i and ii) of the anaerobic n2-fixing bacterium clostridium pasteurianum (cp) and the hydrogenases of the anaerobes megasphaera elsdenii (me) and desulfovibrio vulgaris (strain hildenborough, dv), contain iron-sulfur clusters but not nickel. they are the most active hydrogenases known. all four enzymes in their reduced states give rise to epr signals typical of [4fe-4s]1+ clusters but exhibit novel epr signals in their oxidized states. for example, cp hydrogenase i exhibits ... | 1986 | 3015247 |
| roles of hormonal and nutritional factors in the regulation of rat liver alcohol dehydrogenase activity and ethanol elimination rate in vivo. | fasting reduced the liver alcohol dehydrogenase (adh) activity by 51% (p less than 0.001). insulin, within 2 hr, increased the adh activity found in fasted animals by 28% (p less than 0.02). insulin administration failed to stimulate the reduced adh activity in diabetic rats. however, adh activity in the diabetic-fed rats decreased by 52-54% (p less than 0.001) compared to normal-fed rats regardless of whether they were meal-fed or refed the normal chow. glucagon blocked by 15% (p less than 0.02 ... | 1988 | 3044171 |
| factors affecting the rate of breakdown of bacterial protein in rumen fluid. | 1. the cellular proteins of butyrivibrio fibrisolvens, lactobacillus casei, megasphaera elsdenii, selenomonas ruminantium and streptococcus bovis were labelled by growth in the presence of l-[14c]leucine, and the breakdown of labelled protein was measured in incubations of these bacteria with rumen fluid to which unlabelled 5 mm-l-leucine was added. the rate of protein breakdown was estimated from the rate of release of radioactivity into acid-soluble material. 2. protein breakdown occurred at d ... | 1987 | 3118940 |
| electron-transfer studies involving flavodoxin and a natural redox partner, the iron protein of nitrogenase. conformational constraints on protein-protein interactions and the kinetics of electron transfer within the protein complex. | the kinetics of electron-transfer reactions involving flavodoxins from klebsiella pneumoniae (kpfld), azotobacter chroococcum (acfld), anacystis nidulans (anfld) and megasphaera elsdenii (mefld), the free, mgadp-bound and mgatp-bound forms of the fe protein component of nitrogenase from k. pneumoniae [kp2, kp2(mgadp)2 and kp2(mgatp)2] and na2s2o4 were studied by stopped-flow spectrophotometry. kinetic evidence was obtained for the formation of binary protein complexes involving kpfldsq (semiquin ... | 1988 | 3140782 |
| gene-specific differences in the aflatoxin b1 adduction of chicken erythrocyte chromatin. | mature and immature chicken erythrocyte nuclei were treated with activated aflatoxin b1 (2,3-dichloroaflatoxin b1), producing covalently bound dna adducts. this reaction produces alkali-labile sites in the dna which can be identified by using a variation of the maxam-gilbert sequencing procedure. we determined the aflatoxin b1 accessibility of defined regions of the erythroid genome by using different specific probes and monitoring the disappearance of similar-sized fragments generated by restri ... | 1988 | 3142684 |
| human and bovine endothelial cells synthesize membrane proteins similar to human platelet glycoproteins iib and iiia. | human umbilical vein endothelial (huve) and bovine aortic endothelial (bae) cells in culture were examined to determine whether membrane proteins similar to human platelet glycoproteins (gp) iib and iiia were present. the huve and bae cells were either 125i-surface labeled or metabolically labeled. triton x-100 lysates of labeled cells were immunoprecipitated with polyclonal antibodies prepared against purified human platelet gp iib-iiia complex. two membrane proteins were detected on both huve ... | 1985 | 3161885 |
| large-scale preparation and reconstitution of apo-flavoproteins with special reference to butyryl-coa dehydrogenase from megasphaera elsdenii. hydrophobic-interaction chromatography. | a new method is described for the large-scale reversible dissociation of flavoproteins into apoprotein and prosthetic group using hydrophobic-interaction chromatography. lipoamide dehydrogenase from azotobacter vinelandii and butyryl-coa dehydrogenase from megasphaera elsdenii are selected to demonstrate the usefulness of the method. in contrast to conventional methods, homogeneous preparations of apoproteins in high yields are obtained. the apoproteins show high reconstitutability. the holoenzy ... | 1988 | 3203689 |
| identification of rumen bacteria that anaerobically degrade nitrite. | fifty-one pure strains of rumen bacteria, representing 15 genera, were tested for their ability to metabolize nitrite. twenty-five of the strains, belonging to eight genera, were capable of growth and nitrite metabolism in nitrite-containing medium sterilized by autoclaving. an additional 10 strains showed growth and nitrite metabolism in medium that was autoclaved before the addition of filter-sterilized nitrite. nitrite metabolism was not observed in the remaining 16 strains, and these were al ... | 1988 | 3214813 |
| effects of the ionophores monensin and tetronasin on simulated development of ruminal lactic acidosis in vitro. | a continuous coculture of four ruminal bacteria, megasphaera elsdenii, selenomonas ruminantium, streptococcus bovis, and lactobacillus sp. strain lb17, was used to study the effects of the ionophores monensin and tetronasin on the changes in ruminal microbial ecology that occur during the onset of lactic acidosis. in control incubations, the system simulated the development of lactic acidosis in vivo, with an initial overgrowth of s. bovis when an excess of glucose was added to the fermentor. la ... | 1988 | 3223764 |
| human genome sequence. | | 1988 | 3340144 |
| pharmacokinetic and pathological evaluation of gentamicin in cats given a small intravenous dose repeatedly for five days. | gentamicin was administered to six cats at a dosage of 3 mg/kg of body weight intravenously every 8 h for five days. peak and trough serum gentamicin concentrations were measured after each injection. gentamicin elimination rate and serum half-life were calculated. serum urea nitrogen, creatinine, biochemistry profile, electrolyte, glucose, total protein, and albumin concentrations were measured daily. urinalyses were performed before and after the five-day experimental period. the mean +/- sd p ... | 1988 | 3370552 |
| disappearance of plasmalogen-containing phospholipids in megasphaera elsdenii. | the plasmalogen content of phospholipids isolated from megasphaera elsdenii atcc 17752 decreased markedly in cultures passed serially at intervals of 3 to 6 weeks. from the wild-type ratio of vinyl ether to lipid phosphorus of 0.8, clones were isolated with ratios less than 0.05. clonal analysis, as well as the reproducibility of the phenomenon and the long time course, suggest that the loss of plasmalogens is an adaptive process. although small variations in cell morphology and ratios of end pr ... | 1988 | 3372481 |
| enrichment and isolation of a ruminal bacterium with a very high specific activity of ammonia production. | when mixed ruminal bacteria were inoculated into semicontinuous cultures (25% transfer every other day) containing lactate, dulcitol, pectin, or xylose and trypticase (1 g/liter) as the sole nitrogen source, the specific activity of ammonia production increased. the greatest enrichment was observed with lactate and xylose, and in these cases the specific rate of ammonia production was eightfold higher than that of the ruminal fluid control (approximately 35 nmol of ammonia per mg of protein per ... | 1988 | 3377500 |
| [the effect of ethanol on the fatty liver in zucker fatty rat]. | | 1988 | 3421841 |
| interactions between rumen amylolytic and lactate-utilizing bacteria in growth on starch. | the growth and metabolism of the rumen amylolytic bacteria streptococcus bovis, butyrivibrio fibrisolvens and bacteroides ruminicola, growing in pure cultures and co-cultures with the rumen lactilytic bacteria megasphaera elsdenii and veillonella alcalescens were followed. the interaction of amylolytic bacteria with v. alcalescens represents a simple food chain. the interaction with m. elsdenii is more complex, since there is a simultaneous competition for products of the starch degradation. | 1987 | 3429358 |
| site-specific antibodies define a cleavage site conserved among arenavirus gp-c glycoproteins. | arenaviruses share a common strategy for glycoprotein synthesis and processing in which a mannose-rich precursor glycoprotein, termed gp-c in lymphocytic choriomeningitis virus (lcmv), is posttranslationally processed by oligosaccharide trimming and proteolytic cleavage to yield two structural glycoproteins, gp-1 and gp-2. mapping the orientation and proteolytic cleavage site(s) in such polyproteins has traditionally required direct protein sequencing of one or more of the cleaved products. this ... | 1987 | 3546729 |
| bactericidal effect of hydrogen peroxide on salmonella typhimurium in liquid whole egg. | the effect of hydrogen peroxide on salmonella typhimurium in whole egg was evaluated. the bactericidal effects observed on the test organism at 5 degrees and 20 degrees c were found to be similar. there was a 99% kill in the presence of 0.5% and 1.0% h2o2. addition of the test organism and h2o2 after pre-heating the egg material at 40 degrees c for 15 min caused a rapid kill which was 10,000-fold greater than that produced by h2o2 alone. | 1987 | 3553129 |
| observations on the mechanism and location of ascites reabsorption in man. | animal data indicate that ascites is reabsorbed by a lymphatic mechanism and that these vessels are subdiaphragmatic in location. we evaluated the relative role of lymphatics in ascites reabsorption in man by comparing the ascites clearance and plasma appearance rates of intraperitoneally injected radiolabeled albumin to those of intraperitoneally injected labeled autologous red blood cells, which require, owing to their large size, lymphatic removal, in patients with cirrhosis and ascites. to e ... | 1987 | 3565340 |
| immunospecific red cell binding of iodine 125-labeled immunoglobulin g erythrocyte autoantibodies. | the primary interaction of autoantibodies with red cells has been studied by using labeled autoantibodies. immunoglobulin g red cell autoantibodies obtained from igg antiglobulin-positive normal blood donors were labeled with radioactive iodine and compared with alloanti-d with respect to their properties and binding behavior. iodine 125-labeled igg autoantibody migrated as a single homogeneous peak with the same relative mobility as human igg on sodium dodecyl sulfate-polyacrylamide gel electro ... | 1987 | 3611953 |
| a new right ventricular assist device. the extracorporeal pulsatile assist device (epad). | | 1987 | 3675948 |