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fractionation of membrane vesicles. ii. a method for separation of membrane vesicles bearing different enzymes by free-flow electrophoresis.free-flow electrophoresis was used to subfractionate membrane vesicles from calf thymocyte plasma membranes. the fractionation resulted in a separation of vesicle populations bearing four different enzymes: alkaline nitrophenyl-phosphatase (orthophosphoric-monoester phosphohydrolase (alkalin optimum) ec 3.1.3.1), gamma-glutamyltransferase (ec 2.3.2.2), (mg2+ + na+ + k+)-atpase (atp phosphohydrolase, ec 3.6.1.3) and acyl-coa:lysophosphatidylcholine acyltransferase (acyl-coa:1-acylglycero-3-phosph ...197721691
non-protein constituents in myelin basic protein preparations. 197658970
[ultrastructural observation of the infarctic sequential changes in the cerebral tissue of dog. ii changes in small vessels (author's transl)]. 197991135
condensation--inhibition by 33258-hoechst of centromeric heterochromatin in prematurely condensed mouse chromosomes. 197991529
the effect of glutaurine on the development of amphibian larvae with inhibited rna and protein synthesis.1. glutaurine (gamma-l-glutamyl-taurine) isolated from bovine parathyroid and later synthetized retards the metamorphosis of rana arvalis. 2. from day 13 of treatment glutaurine moderates the metamorphosis inhibiting effect of antibiotics acting at the transcription and translation level.197994855
pbb fed to immature chickens: its effect on organ weights and function and on the cardiovascular system.in a series of polybrominated biphenyl (pbb) feeding trials in white leghorn cockerel chicks, the effects of pbb on various physiological parameters and organ weights were determined. these measurements included: growth, thyroid function, ecg, cardiac output, blood pressure, hematocrit, erythropoietin levels, and spleen, bursa of fabricius, thyroid, testes, comb, liver weights. in addition, tissues were histologically examined. pbb, in the commercial grade form of hexabromobiphenyl, was administ ...1978209983
induction of differentiation in human promyelocytic leukemia cells by tumor promoters.phorbol diester tumor promoters and the promoter mezerein convert human promyelocytic leukemia cells in culture into adherent, nonproliferating cells with many of the characteristics of macrophages. other types of promoters such as anthralin, phenobarbital, and saccharin do not have this effect. various compounds that can inhibit some of the biological and biochemical effects of tumor promoters do not interfere with the induction of cell adherence and differentiation by the effective promoters.1979286421
an unexplained high incidence of calcified lesions in dog kidneys. 19761265979
synaptic plasticity in the hypoglossal nucleus of female canaries: structural correlates of season, hemisphere, and testosterone treatment.the caudal portion of the hypoglossal nucleus (nxiits) contains the motor neurons that control the syrinx in songbirds. in canaries, song occurs seasonally, is principally produced by males, and appears to be produced predominantly by muscles on the left side of the syrinx. the present study measures the effect of seasonal change and manipulation of testosterone levels on synapse number and morphology in nxiits in adult female canaries. we find that synapse density is lower in testosterone-treat ...19892757585
synaptic plasticity in the hypoglossal nucleus of female canaries: structural correlates of season, hemisphere, and testosterone treatment.the caudal portion of the hypoglossal nucleus (nxiits) contains the motor neurons that control the syrinx in songbirds. in canaries, song occurs seasonally, is principally produced by males, and appears to be produced predominantly by muscles on the left side of the syrinx. the present study measures the effect of seasonal change and manipulation of testosterone levels on synapse number and morphology in nxiits in adult female canaries. we find that synapse density is lower in testosterone-treat ...19892757585
[therapy of aids].therapeutic strategies in the management of aids should aim at an inhibition of the replication and spread of hiv; the reconstitution of immune functions; an effective chemotherapy of opportunistic infections and tumors; and the prevention of new opportunistic infections. at present, these goals can be only partially achieved and where beneficial therapeutic results are obtained they are only transient in nature. antiretroviral therapy and chemotherapy of infections have, however, resulted in so ...19882849247
cmv, hiv, and the pancreas. 19882901600
the t-antigen-binding domain of the simian virus 40 core origin of replication.the simian virus 40 origin of replication contains a 27-base-pair palindrome with the sequence 5'-ca-gaggc-c-gaggc-g-gcctc-g-gcctc-tg-3'. the four 5'-gaggc-3'/5'-gcctc-3' pentanucleotides are known contact sites for simian virus 40 t-antigen binding in vitro. we used oligonucleotide-directed cassette mutagenesis to identify features of this palindrome that are important for the initiation of dna replication in vivo. each base pair of a pentanucleotide is crucial for dna replication. in contrast, ...19873035215
[pseudomonas aeruginosa: immunotypes and drug resistance].serotyping of pseudomonas aeruginosa by fisher's schema has been used to study the prevalence of particular immunotypes among strains of p. aeruginosa obtained from clinical specimens. about 78% of the isolates were serotypable and about 22% were not. immunotypes 2 and 6 turned out to be the most prevalent, whereas immunotypes 5 and 7 were the least frequent. immunotypes 2, 5, 6 and 7 did not vary significantly as far as frequency in the various sources is concerned, with the exception of immuno ...19863119410
characterization of a-i-containing lipoproteins in subjects with a-i milano variant.the a-i milano variant of apolipoprotein a-i (a-im), by virtue of its arg-173----cys substitution, is capable of forming a disulfide bond with the 77-amino-acid apolipoprotein a-ii polypeptide (a-iis) as well as with itself to produce dimers, a-im/a-iis and a-im/a-im, respectively. a-i-containing lipoproteins (lp): particles with a-ii (lp(a-i with a-11)) and particles without a-ii (lp(a-i without a-ii)) in the plasma of two nonhyperlipidemic a-im carriers were investigated to determine the effec ...19883129016
serum albumin adducts in the molecular epidemiology of aflatoxin carcinogenesis: correlation with aflatoxin b1 intake and urinary excretion of aflatoxin m1.aflatoxin-serum albumin adducts in the blood of 42 residents of guangxi province, people's republic of china, were determined and compared with intake of aflatoxin b1 (afb1) and excretion of aflatoxin m1 (afm1) in urine. blood specimens were obtained during the same period that urine was collected and that diet was sampled. serum albumin was isolated from blood by affinity chromatography on reactive blue 2-sepharose and subjected to enzymatic proteolysis using pronase. immunoreactive products we ...19883133131
isolation and characterization of chondroitin 6-sulfate proteoglycans present in the extracellular matrix of rabbit bone marrow.the authors' previous studies showed that the glycosaminoglycans present in rabbit bone marrow were composed of chondroitin 6-sulfate (79%) and hyaluronic acid (16%). immunohistochemically the chondroitin 6-sulfate was demonstrated to be in bone marrow matrix constructing hematopoietic microenvironment. in this study the authors isolated and characterized these glycosaminoglycans in their macromolecular form (i.e., proteoglycans). bone marrow of 3-month-old rabbits was defatted with organic solv ...19883135865
[demonstration of a sex differentiation in the content of androgen receptors in the liver cytosol of rats].the paper is concerned with differential quantification of the level of androgen receptors (ar) in the liver cytosol of male and female rats as well as in the prostatic cytosol. ar affinity to synthetic androgen r1881 was shown to be similar in all investigated tissues. in male rats the ar content in the liver cytosol was 20.5 +/- 2.1 fmol per 1 mg of protein, lower (p less than 0.05) than in the prostatic cytosol (30.8 +/- 2.5 fmol per 1 mg of protein). sex differentiation of the liver ar conte ...19883264067
the unusual translational initiation codon auu limits the expression of the infc (initiation factor if3) gene of escherichia coli.the expression of infc, the structural gene for translational initiation factor if3, has been studied in different constructs under the control of the lambda pl and tac promoters. the amount of synthesized if3 has been determined by a quantitative functional test and the levels of if3-specific mrna have been estimated. the synthesis of if3 is strongly enhanced when the unusual auu initiation codon is changed to aug by site-directed mutagenesis. removal of the sequence upstream from the start cod ...19873302616
neuromuscular block by verapamil and diltiazem and inhibition of acetylcholine release.the effects of ca2+-channel antagonists, verapamil, diltiazem and nifedipine, on the neuromuscular transmission were studied in the isolated mouse phrenic nerve-diaphragm preparations. all 3 drugs increased the twitch response evoked by direct single stimulation at 10-100 microm. the neuromuscular transmission at 0.1 hz was blocked by verapamil and diltiazem, but not by nifedipine, only at very high concentrations (greater than or equal to 100 microm). in the time course of block, no endplate po ...19883409016
tubular lysosomes accompany stimulated pinocytosis in macrophages.a network of tubular lysosomes extends through the cytoplasm of j774.2 macrophages and phorbol ester-treated mouse peritoneal macrophages. the presence of this network is dependent upon the integrity of cytoplasmic microtubules and correlates with high cellular rates of accumulation of lucifer yellow (ly), a marker of fluid phase pinocytosis. we tested the hypothesis that the efficiency of ly transfer between the pinosomal and lysosomal compartments is increased in the presence of tubular lysoso ...19873571329
assessment of the safety of chemicals administered intravenously in the neonatal rat.a 3-day-old neonatal rat model for the safety assessment of various drugs, chemicals, and nutrients administered intravenously is described. this model was used to assess the safety of di(2-ethylhexyl) phthalate (dehp), cyclohexanone, and a 3:1 mixture of medium and long-chain triglyceride emulsions following subchronic administration using the intravenous route. the administration of dehp at dosage of 164.8 mg/kg for 18 consecutive days resulted in a small but statistically significant increase ...19873603403
assessment of the safety of chemicals administered intravenously in the neonatal rat.a 3-day-old neonatal rat model for the safety assessment of various drugs, chemicals, and nutrients administered intravenously is described. this model was used to assess the safety of di(2-ethylhexyl) phthalate (dehp), cyclohexanone, and a 3:1 mixture of medium and long-chain triglyceride emulsions following subchronic administration using the intravenous route. the administration of dehp at dosage of 164.8 mg/kg for 18 consecutive days resulted in a small but statistically significant increase ...19873603403
immunospecific red cell binding of iodine 125-labeled immunoglobulin g erythrocyte autoantibodies.the primary interaction of autoantibodies with red cells has been studied by using labeled autoantibodies. immunoglobulin g red cell autoantibodies obtained from igg antiglobulin-positive normal blood donors were labeled with radioactive iodine and compared with alloanti-d with respect to their properties and binding behavior. iodine 125-labeled igg autoantibody migrated as a single homogeneous peak with the same relative mobility as human igg on sodium dodecyl sulfate-polyacrylamide gel electro ...19873611953
aminopeptidase p from bovine lung: solubilization, properties, and potential role in bradykinin degradation.aminopeptidase p was solubilized from bovine lung by sodium deoxycholate extraction of salt-washed, delipidated lung acetone powders. hydrolysis of the standard aminopeptidase p substrate, gly-pro-hyp, as well as cleavage of arg-pro-pro and the arg1-pro2 bond of bradykinin, co-eluted from a mono q anion exchange column and demonstrated identical inhibitory profiles suggesting that all activities were functions of the same enzyme. the metal chelator, 1,10-phenanthroline, completely inhibited acti ...19873627107
induction of rheumatoid factors in mice by immune complexes of bacterial lipopolysaccharide with mouse igg antibody.administration of 2,4,6-trinitrophenylated e. coli lipopolysaccharide (tnp-lps) complexed with mouse igg antibody to tnp specifically gave rise to a marked production of rheumatoid-like factors (rf) in the recipient mice, in contrast to the low and nonspecific rf production via polyclonal b cell activation by the same dosage of lps or tnp-lps alone. the rf activity induced by the lps immune complexes was associated with both igg and igm and directed primarily to the c gamma 2 region as judged by ...19863753937
urinary glutathione-s-transferase in cisplatin nephrotoxicity in the rat.rats given a single toxic dose of cisplatin all developed detectable glutathione-s-transferase activity in their urine between the third and fifth day after injection of cisplatin, simultaneously with the decreased urine osmolality and increased urine volume characteristic of cisplatin nephrotoxicity. peak urinary glutathione-s-transferase levels occurred at the same time as maximal serum creatinine levels, and there was a significant statistical correlation between these two variables. these fi ...19863760785
clues to the early diagnosis of mycobacterium avium-intracellulare infection in patients with acquired immunodeficiency syndrome.four patients with acquired immunodeficiency syndrome with bacillemia from mycobacterium avium-intracellulare presented with early pathologic clues of a disseminated mycobacterial infection. all had persistent fevers with negative diagnostic workups for other usual pathogens seen in patients with acquired immunodeficiency syndrome. two patients had prolonged clearance of the bacillemia on a drug regimen of ansamycin, clofazimine, and amikacin sulfate.19853838662
[studies on the feedback regulation of pulsatile luteinizing hormone secretion: effect of ovarian steroid implantation into the forebrain and basal hypothalamus on pulsatile lh secretion in ovariectomized rats].recent studies have demonstrated that ovarian steroids are involved in the regulation of pulsatile lh secretion. in order to identify the site of action of ovarian steroids in modulating pulsatile lh secretion, the effect of local administration of estradiol benzoate (eb) or progesterone (p) into various brain regions on the characteristic of lh pulses was investigated in ovariectomized rats. female rats of the wistar strain were ovariectomized about 3-4 weeks before the experiment. blood sample ...19853996684
[studies on the feedback regulation of pulsatile luteinizing hormone secretion: effect of ovarian steroid implantation into the forebrain and basal hypothalamus on pulsatile lh secretion in ovariectomized rats].recent studies have demonstrated that ovarian steroids are involved in the regulation of pulsatile lh secretion. in order to identify the site of action of ovarian steroids in modulating pulsatile lh secretion, the effect of local administration of estradiol benzoate (eb) or progesterone (p) into various brain regions on the characteristic of lh pulses was investigated in ovariectomized rats. female rats of the wistar strain were ovariectomized about 3-4 weeks before the experiment. blood sample ...19853996684
[early penicillin treatment can prevent cns symptoms in tick-transmitted borrelia meningitis]. 19854046663
y-chromatin fluorescence in human buccal smear. 19744138992
[leptotrichia buccalis--culture, biochemistry and antibiotics]. 19744154653
ultrastructure of the ageing retinal vascular system, with special reference to quantitative and qualitative changes of capillary basement membranes. 19734747160
plasma diamine oxidase activity after single or chronic administrations of histamine. 19694981910
effect of calcium ion on s-100, a protein of the nervous system. 19694982046
cerebral perfusion pressure: a hemodynamic mechanism of mannitol and the postmannitol hemogram.sixteen patients each received infusions of 1 g of mannitol per kg over 5 to 10 minutes, and serial determinations of intracranial pressure (icp), systemic arterial blood pressure (sabp), central venous pressure, cerebral perfusion pressure (cpp), hematocrit, hemoglobin, serum na+, k+, osmolarity, and fluid balance were carried out for 4 hours. urine output was replaced volume for volume with 5% dextrose in 0.45% nacl solution. we tested the hypothesis that patients with high (greater than or eq ...20103116451
[primary transport of acute patients. optimal use of rescue resources exemplified by the canton of graubünden]. 20113927607
localisation of nucleolus-organizing regions in interphase cells.a technique based on the use of silver solutions, which selectively stains the nucleolus-organising regions (nors) in chromosomes, was applied to interphase ehrlich tumour cells. the results indicate that nucleolar fibrillar centres correspond to the nors.199991444
glossary of terms for thermal physiology. 20164765838
total parenteral nutrition. 1998107267
[current approaches to immunological diagnosis of autoimmune and immune complex diseases]. 20102974642
the isotypic circuit: immunoglobulins, fc receptors and immunoglobulin binding factors. 20113333781
breast biopsy technique: a counterplea for breast conservation. 20133787301
multiple dose insulin regimen using the novopen. 20133534657
[re-evaluation of nursing procedures for the prevention of infections. prevention of infections during the application of intravenous hyperalimentation (ivh)]. 20113143844
[what is new in nursing education? it is worse not to want to listen than to be deaf]. 1998107529
creatine-kinase and ck-mb isoenzyme activity in serum of patients after surgical operations, polytrauma and other damage to skeletal muscle. 2004535155
antimicrobial prophylaxis in immunocompromised patients.the results of a randomised, prospective trial to investigate the efficacy of prophylactic treatment with ofloxacin during granulocytopenia after cytostatic treatment are presented. 42 patients with metastasised testicular germ-cell tumours entered the study. cytostatic treatment consisted of at least 4 courses, 2 of which were succeeded by prophylactic treatment with ofloxacin. three patients undergoing prophylactic treatment developed fever. fever occurred in 16 patients during control phases ...20123325256
current operating statistics. 2003152474
chest pain of esophageal origin. 20073864445
chest pain of esophageal origin. 20073864445
bile duct adenomas as liver nodules.bile duct adenomas can be difficult to differentiate at laparotomy from small metastatic tumor nodules in the liver. this can present a problem to the pathologist and surgeon relative to the advisability of attempted curative vs pallative surgery. we report two such cases. review of our autopsy materials shows the incidence of bile duct adenoma to be higher than usually reported. even though they all present as grayish-white, firm nodules, usually frozen section will reveal the true nature of th ...1994205188
[activities of auxiliary medical personnel in medical and surgical emergencies]. 1998106533
comparative study of c5a plasma levels with different hemodialysis membranes using an enzyme-linked immunosorbent assay.using a specific and sensitive elisa for c5a, the present study shows that predialysis levels of c5a in end-stage renal disease patients are not elevated and that all membranes studied (cuprophan, hemophan, gambrane and hydrophilic polysulfone) cause significant increases of c5a plasma levels albeit to different degrees. higher increases of c5a were accompanied by larger decreases of circulating granulocytes and monocytes. dialyzers with a newly introduced modified regenerated cellulose membrane ...20102918943
haemorrhagic shock encephalopathic syndrome and neurodevelopmental delay. 20102863462
[aids--even children are affected]. 20133807478
auranofin-associated colitis and eosinophilia.gold compounds, often used in the treatment of rheumatoid arthritis, have been associated with gastrointestinal disturbances in some patients. use of auranofin, an oral gold preparation, in a 50-year-old woman with rheumatoid arthritis resulted in diarrhea, abdominal tenderness, nausea, and vomiting, which persisted despite discontinuation of auranofin therapy. the presumptive diagnosis was gold-induced colitis and eosinophilia. administration of cromolyn sodium provided relief. although this co ...20133807438
6 beta-hydroxycortisol in serum and urine as determined by enzyme immunoassay on microtitre plates.we modified our previous enzyme immunoassay (clin chim acta 1986;157:267-76) for estimating 6 beta-hydroxycortisol (6 beta-ohf) to provide a more routine procedure for measuring it in urine and to increase sensitivity for its measurement in serum. precision, analytical recovery, specificity, and detection limit are reported. 6 beta-ohf and 17-hydroxycorticosteroids in 24-h and 4-h (08:00-12:00 h) urine collections from healthy volunteers showed no significant day-to-day differences in excretion ...20133536172
university dedicates pechan health center. 20144183296
pharmacotherapeutical census in a psychiatric hospital (comparison with the year 1966). 20165040170
the vanadium and selected metal contents of some ascidians. 20164822766
[anterior dislocation of the atlas, an unrecognized case]. 20164771823
[demonstration and reaction of 2-ethoxy-3-acetoacetyl-chinoxaline. 42. studies on 4-pyrones]. 20164730141
corneal ultrastructural changes in fabry's disease.a case of fabry's disease is reported. the findings of the corneal alterations with the light microscope as well as with the electron microscope are described. the diffuse corneal haziness seems to be due to small intracellular osmiophilic inclusions of the epithelium. there seems also to be a relation between the giant inclusions in the corneal epithelium, not yet described, and the clinical presence of punctiform grey opacities. the pathogenesis of the whorl-like opacities remains unsolved.1988211468
letter: minority students. 19931134547
[histomorpholoigcal findings on 2252 cases of genital blood loss]. 199991340
lymphocytotoxic antibodies in hydralazine-induced lupus erythematosus. 200192663
assessment of placental function in normal and pathological pregnancies by estimation of plasma oestradiol levels after injection of dehydroepiandrosterone sulphate.a test of placental function was assessed by measurement of plasma oestradiol levels in 32 patients after intravenous injection of 50 mg dehydroepiandrosterone sulphate (dhas). every pregnant patient showed a rise in plasma oestradiol after dhas; two puerperal patients showed no rise. it was not possible to distinguish the variable response in normal pregnancy from that in patients with severe hypertension and proteinuria or with retarded intrauterine fetal growth. the test was of no value in on ...2002126694
on the amount of (na+ + k+)-atpase available for transepithelial sodium ion transport in the amphibian skin.pretreatment of frog skin epithelium homogenates with sodium dodecyl sulphate in the presence of atp reveals levels of ouabain-sensitive atpase activity usually higher and occasionally far higher than those required to sustain maximum rates of na+ transport. this supports the view that na+ transport involves only a fraction of the epithelial cells.2002134748
monoclonal antibodies and complement purged autograft in burkitt lymphoma and lymphoblastic leukemia. 20092713555
[prognostic significance of pretreatment ct scans in supratentorial astrocytomas receiving radiotherapy].pretreatment brain ct scans of 61 patients receiving radiation and chemotherapy for supratentorial gliomas (23 low-grade astrocytomas, 21 grade iii astrocytomas and 17 glioblastomas) were reviewed, and their prognoses were analyzed. almost all gliomas with no cea (contrast enhanced area) were low-grade, and their prognosis was promising. benign and malignant gliomas with regular cea were found in equal proportions, and their prognosis was relatively good. a majority of cases with irregular or ri ...20103035252
[basal serum production of gamma-interferon in subjects with gramineous allergic rhinosinusitis, in carriers of cancer of the larynx and in normal control subjects: study with specific monoclonal antibody. preliminary data].the authors report preliminary data about if-gamma (interferon-gamma) basal serum production tested by monoclonal antibody in three groups of 25 patients: alpha (normal controls), beta (ragweed hayfever patients) and gamma (laryngeal carcinoma patients pt3-4, pn0----3, pm0 by uicc). the if-gamma mean value in ragweed hayfever patients is about five times higher then normal controls. these data are highly statistically significative. moreover no value of if-gamma basal serum production is tested ...20113119408
the relationship between renal metabolism and proximal tubule transport during ontogeny.the proximal tubules of newborn and adult animals reabsorb a similar fraction of the filtered load of na+ and h2o (65%-70%). in tubules from adult animals, transcellular, active na+ reabsorption accounts for one-third of the total, while two-thirds occur passively through the paracellular pathway, driven by hydrostatic and oncotic forces (one-third) and by cell-generated effective osmotic and ionic gradients (one-third). since two-thirds of the na+ is reabsorbed passively and does not require en ...20113153041
a novel mitotic spindle pole component that originates from the cytoplasm during prophase.several unique aspects of mitotic spindle formation have been revealed by investigation of an autoantibody present in the serum of a patient with the crest (calcinosis, raynaud's phenomenon, esophageal dysmotility, schlerodacytyly, and telangiectasias) syndrome. this antibody was previously shown to label at the spindle poles of metaphase and anaphase cells and to be absent from interphase cells. we show here that the serum stained discrete cytoplasmic foci in early prophase cells and only later ...20123536956
a novel mitotic spindle pole component that originates from the cytoplasm during prophase.several unique aspects of mitotic spindle formation have been revealed by investigation of an autoantibody present in the serum of a patient with the crest (calcinosis, raynaud's phenomenon, esophageal dysmotility, schlerodacytyly, and telangiectasias) syndrome. this antibody was previously shown to label at the spindle poles of metaphase and anaphase cells and to be absent from interphase cells. we show here that the serum stained discrete cytoplasmic foci in early prophase cells and only later ...20123536956
dental prepayment, group practice and hmos. 20144535659
pre-hospital coronary care. 20144497605
vaginal hysterectomy for sterilization.vaginal hysterectomy for sterilization was performed on 115 patients. the heaney technique was used by nine gynecologists, using lidocaine and adrenaline. the average age of patients was 32.2 years and average parity 3.2. significant unsuspected pathology was found after surgery in 21 patients. one postoperative death occurred due to a brain stem infarction. complications after hospitalization were vaginal cuff bleeding and urinary tract infection. lowered morbidity of patients on oral contracep ...20155023260
[proposals for systematic periodontal treatment]. 20154510704
[synthetic corticotropin in the treatment of nephrosis in childhood]. 20154325475
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