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[staining of sulfate-reducing bacteria with hexacyanoferrat-compounds (cultural and cytochemical evidence of fe) (author's transl)].hexacyanoferrat-compounds are suitable 1. to indicate the valence of fe-ions in cultures of desulfuricants (desulfovibrio spec., desulfotomaculum) in fluid and solid medium before and after inoculation; 2. stain directly the sulfate-reducing bacteria in presence of fe-ions and give a colour-reaction of those parts of the cell containing fe-compounds, e.g. fes. therefore they act as a cytochemical indicator system. the chemical base of the reactions are as follows: (1) 4hs + so42 leads to h2s + 2 ...197877605
study on dissimilatory reduction of sulphates.a new strain of sulphate reducing bacteria was isolated from swampy forest soil. after 120 h reduction, sulphate conversion attained 100%, the molar ratio of the consumed lactate and reduced sulphate amounted to 2:1. this confirms the reduction mechanism proposed by senez (1951).197987119
the effect of organic substrate concentration on activity for microbiological reduction of sulfates.lactate, pyruvate, malate and alpha-glycerophosphate are excellent sources of energy and structural carbon for desulfovibrio vulgaris. a fully stoichiometric course of the sulfate reduction process has been observed. a simplified equation of the reaction describing the respiration process of the above bacteria in the presence of alpha-glycerophosphate is proposed.197992173
physiological and morphological observations on thiovulum sp.cell suspensions of thiovulum sp., collected from enrichment cultures, were grown, maintained, and harvested for periods up to 7 months. in open-flow cultures run with aerated seawater, a continuous supply of hydrogen sulfide was provided by diffusion through a semipermeable membrane from either a live culture of desulfovibrio esturaii, neutralized sodium sulfide, or a n2-h2s gas mixture. attempts to grow thiovulum in pure culture failed despite variation in concentrations of dissolved oxygen an ...1978101531
siroheme: methods of isolation and characterization. 1978149890
superoxide dismutase in some obligately anaerobic bacteria. 1975163764
biochemical studies on sulfate-reducing bacteria. xiv. enzyme levels of adenylylsulfate reductase, inorganic pyrophosphatase, sulfite reductase, hydrogenase, and adenosine triphosphatase in cells grown on sulfate, sulfite, and thiosulfate.sulfate-reducing bacteria, desulfovibrio vulgaris, strain miyazaki, were grown on either sulfate, sulfite, or thiosulfate as the terminal electron acceptor. better growth was observed on sulfite and less growth on thiosulfate than on sulfate. enzyme levels of adenylylsulfate (aps) reductase [ec 1.8.99.2], reductant-activated inorganic pyrophosphatase [ec 3.6.1.1], sulfite reductase [ec 1.8.99.1] (desulfoviridin), hydrogenase [ec 1.12.2.1], and mg2+-activated atpase [ec 3.6.1.3] were compared in ...1975175050
a molybdenum-containing iron-sulphur protein from desulphovibrio gigas. 1976186061
redox states of cytochrome c3 in the absence and presence of ferredoxin. 1977200473
microbial gas metabolism. 1976188319
rubredoxin. 1978213684
purification of electron-transfer components from sulfate-reducing bacteria. 1978213690
kinetics of reduction of the cytochrome c3 from desulvovibrio vulgaris. 1978214075
preliminary crystallographic study on cytochrome c3 of desulfovibrio desulfuricans (strain norway). 1976181583
properties of purified hydrogenase from the particulate fraction of desulfovibrio vulgaris, miyazaki.the properties of purified hydrogenase [ec 1.12.2.1] solubilized from particulate fraction of sonicated desulfovibrio vulgaris cells are described. the enzyme was a brownish iron-sulfur protein of molecular weight 89,000, composed of two different subunits (mol. wt.: 28,000 and 59,000), and it contained 7-9 iron atoms and 7-8 labile sulfide ions. molybdenum was not detected in the preparation. the absorption spectrum of the enzyme was characteristic of iron-sulfur proteins. the millimolar absorb ...1976181372
a new assay method for hydrogenase based on an enzymic electrode reaction. the enzymic electric cell method.a new assay method for hydrogenase [ec 1.12.2.1] based on the enzymic electrode reaction of h2-h+ equilibrium has been established. the method is based on the experimental fact that the short-circuit current of the electric cell composed of an electrode with hydrogenase and methylviologen as the mediator of h2-h+ equilibrium and a saturated calomel electrode as the counter electrode, is practically proportional to the amount of hydrogenase in the cell. the new method is referred to as the "enzym ...1975178640
product analysis of bisulfite reductase activity isolated from desulfovibrio vulgaris.bisulfite reductase was purified from extracts of desulfovibrio vulgaris. by colorimetric analyses trithionate was found to be the major product, being formed in quantities 5 to 10 times more than two other detectable products, thiosulfate and sulfide. when [35s]bisulfite was used as the substrate, all three products were radioactively labeled. degradation of [35s]trithionate showed that all of its sulfur atoms were equally labeled. in contrast, [35s]thiosulfate contained virtually all of the ra ...1976177403
localized intracellular polyphosphate formation by desulfovibrio gigas.the dissimilatory sulphate-reducing bacterium desulfovibrio gigas, frequently sub-cultured, often contained spherical granules which stained metachromatically with some basic dyes. the granules were examined in situ by transmission electron microscopy of whole organisms and thin sections. the granules were isolated from broken bacteria as a water-insoluble, non-crystalline, white material containing magnesium, phosphorus and organic carbon, but devoid of sulphur and nitrogen. the molar ratio of ...197550405
[hexacyanoferrate-compounds as indicator substances for the efficacy of antimicrobial agents to desulfuricants (author's transl)].hexacyanoferrate-compounds are suitable to indicate the efficacy of antimicrobial substances to sulfate-reducing bacteria. they act as a cytochemical indicator system. (stübner, 9), e. g. k3feiii(cn)6 showes fes formation before any visible blackening of the medium. by using hexacyanoferrate compounds it is easier to determine the efficacy of antimicrobial agents. in addition a microscopical control is recommended to detect damaged or avital bacteria in the limiting concentrations of the agents.197944792
redox studies on rubredoxins from sulphate and sulphur reducing bacteria. 1979228978
hydrogen bonding of flavoprotein. i. effect of hydrogen bonding on electronic spectra of flavoprotein.the effect of hydrogen bonding on the transition energy and the oscillator strength of the isoalloxazine nucleus of flavins was studied by the molecular orbital method. among the possible hydrogen bondings examined, characteristic spectral shifts were found for the hydrogen bondings at n(1) and n(5) of the nucleus. the hydrogen bonding at n(1) resulted in the shift of the first absorption band towards blue and that of the second one towards red. on the other hand, the hydrogen bonding at n(5) re ...197828779
structure-function relationship in hemoproteins: the role of cytochrome c3 in the reduction of colloidal sulfur by sulfate-reducing bacteria.cytochromes c3 of different strains of sulfate-reducing bacteria have been purified and tested for their capacity to reduce colloidal sulfur to hydrogen sulfide. the results are in good agreement with the activities reported for the whole cells. cytochrome c3 is the sulfur reductase of some strains of sulfate-reducing bacteria such as desulfovibrio desulfuricans norway 4 and sulfate-reducing bacterium strain 9974 from which the sulfur reductase activity can be purified with the cytochrome c3. in ...1979229785
growth yields and growth rates of desulfovibrio vulgaris (marburg) growing on hydrogen plus sulfate and hydrogen plus thiosulfate as the sole energy sources. 197828099
studies on the reaction of imidazole with cytochrome c3 from desulfovibrio vulgaris. 197826395
effect of pressure upon the fluorescence of various flavodoxins.the effects of hydrostatic pressure in the range of 10(-3) to 11 kbar on the fluorescence of flavodoxins from peptostreptococcus elsdenii, desulfovibrio vulgaris, azotobacter vinelandii, and clostridium mp were investigated. the first three flavoproteins showed under high pressure enhancements of flavin fluorescence of over 50 times resulting from the release of flavin mononucleotide from the protein complex. the clostridial flavodoxin showed a very much smaller fluorescence change. at ph 7.5 th ...197720943
the tricarboxylic and acid pathway in desulfovibrio.strains of two species of desulfovibrio were examined for enzymes of the tricarboxylic acid cycle and related pathways. pyruvate carboxylase (ec6.4.1.1) is present, and alpha-ketoglutarate is formed via the tricarboxylic acids. glutamate, but not succinyl-coa, arises from alpha-ketoglutarate. a pathway exists from pyruvate by malic enzyme (ec 1.1.1.39) activity to malate, then fumarate and succinate, again with no evidence of succinyl-coa formation. the enzymes concerned with metabolism of these ...197718274
variations in the spectrum of desulfoviridin from desulfovibrio gigas.desulfoviridin preparations from d. gigas showed variations in the position of the absorption maximum the beta-peak) in the 580-nm region of the specturm. on treatment with na2s2o4 a preparation with a beta-peak at 585 nm was affected rapidly, the 585-nm peak shifting to the 596-nm region; this was partially reversed by k3fe(cn)6. treatment of the original preparation with k3fe(cn)6 resulted in a shift of the beta-peak to 582-583 nm. desulfoviridins with beta-peaks from 580 to 583 nm were not ra ...197614608
investigations on the microbial reduction of sulphates. 1975235829
non-heme iron proteins. the amino acid sequence of rubredoxin from desulfovibrio vulgaris.a non-heme iron protein, rubredoxin has been isolated from the sulfate-reducing bacterium, desulfovibrio vulgaris, strain hildenborough. the complete amino acid sequence has been established. the 52 amino acid residues of the protein were aligned with the aid of tryptic and chymotryptic peptides and of a fragment produced by cleavage of the asn-gly bond (22-23) by hydroxylamine. the sequence of the first 30 residues of the molecule was determined using an automatic sequenator, after removal of t ...19767308
resonance raman scattering from hemoproteins. effects of ligands upon the raman spectra of various c-type cytochromes.resonance raman spectra were measured for various c-type cytochromes (mammalian cytochrome c, bacterial cytochrome c3, algal photosynthetic cytochrome f, and alkylated cytochrome c) and a b-type cytochrome (cytochrome b5) in their reduced and oxidized states. (1) for ferrous alkylated cytochrome c, a raman line sensitive to the replacement of an axial ligand of the heme iron uas found around 1540 cm=1. this ligand-sensitive raman line indicated the transition from acidic (1545 cm-1) to alkaline ...19752584
oxidation-reduction studies of the mo-(2fe-2s) protein from desulfovibrio gigas.potentiometric titration followed by e.p.r. measurements were used to determine the midpoint reduction potentials of the redox centres of a molybdenum-containing iron-sulphur protein previously isolated from desulfovibrio gigas, a sulphate-reducing bacterium (moura, xavier, bruschi, le gall, hall & cammack (1976) biochem. biophys. res. commun. 728 782-789; moura, xavier, bruschi, le gall & cabral (1977) j. less common metals 54, 555-562). the iron-sulphur centres could readily be distinguished i ...1978212012
nmr studies of electron carrier proteins from sulphate reducing bacteria.the sulphate-reducing bacteria have a complex electron transfer system which leads to the reduction of sulphate by oxidation of either organic substrates or molecular hydrogen. these bacteria can either produce or consume molecular hydrogen. the central part of this electron pathway for desulovibrio gigas is constituted by hydrogenase (3 x (4fe-4s)). cytochrome c3 (4 haems with different redox potentials) and a one (4fe-4s) cluster ferredoxin. this ferredoxin is isolated in different oligomeric ...1978208661
epr determination of the oxidation-reduction potentials of the hemes in cytochrome c3 from desulfovibrio vulgaris.epr spectroscopy in conjunction with oxidation-reduction potentiometry has been used to determine the half-reduction potentials of the four hemes of cytochrome c3. as predicted, the four hemes of cytochrome c3 have different mid-point potentials. the em values are: heme i,--284 mv; heme ii,--310 mv; heme iii,--324 mv and heme iv,--319 mv. the n-values in each case was near one.1978208660
characterization of the periplasmic hydrogenase from desulfovibrio gigas. 1978208565
a comparative spectroscopic study of two non-haem iron proteins lacking labile sulphide from desulphovibrio gigas.the ultraviolet visible, and near infrared spectrum of a two-iron protein from desulphovibrio gigas, a new type of non-haem iron protein lacking labile sulphide, is compared with that of d. gigas rubredoxin. the charge transfer band maxima of rubredoxin at 495 and 565 nm are less separated in the new protein implying a higher symmetry of the two iron centres. the existence of a spin-spin interaction between the two iron centres in the new protein is suggested by the magnetic susceptibility measu ...1978205263
dimethyl sulfoxide as an electron acceptor for anaerobic growth.the isolation from lake mud of a bacterium which can use dimethyl sulfoxide (dmso) as an electron acceptor for growth is described. the isolate, called strain dl-1, was a small, gram negative, non-motile spiral. the sole product of dmso reduction was dimethyl sulfide (dms). other electron acceptors used by the isolate included sulfite, thiosulfate, elemental sulfur, methionine sulfoxide, tetramethylene sulfoxide, nitrate, and oxygen (microaerophilically). sulfate was not reduced and could not ev ...1978414686
structural homology of cytochromes c.cytochromes c from many eukaryotic and diverse prokaryotic organisms have been investigated and compared using high-resolution nuclear magnetic resonance spectroscopy. resonances have been assigned to a large number of specific groups, mostly in the immediate environment of the heme. this information, together with sequence data, has allowed a comparison of the heme environment and protein conformation for these cytochromes. all mitochondrial cytochromes c are found to be very similar to the cyt ...1978203462
[fixation of molecular nitrogen by sulfate-reducing bacteria from petroleum strata].the activity of nitrogen fixation by the museum strains of sulfate reducing bacteria isolated from oil deposits was studied using the acetylene technique. the mesophilic sulpfate reducing bacteria belonging to the species desulfovibrio africanus 2372 and d. baculatus x were found to have a high nitrogenase activity. d desulfuricans subsp. aestuarii 2198 reduced acetylene at a low rate. the thermophilic sulfate reducing cultures of d. thermophilus 7, desulfotomaculum nigrificans 781 and dm. nigri ...1979423802
characterization of a novel thiosulfate-forming enzyme isolated from desulfovibrio vulgaris.an enzyme that formed thiosulfate from bisulfite and trithionate was purified from extracts of desulfovibrio vulgaris. this enzyme, designated as "thiosulfate-forming" enzyme, required the presence of both bisulfite and trithionate. various 35s-labeling studies showed that thiosulfate was formed from bisulfite and the inner sulfur atom of trithionate. this involved a nucleophilic attack by the bisulfite ion, resulting in the displacement of the two outer sulfonate groups of trithionate that recy ...1977199572
cytochrome c-551.5 (c7) from desulfuromonas acetoxidans.cytochrome c-551.5 of the anaerobic sulfur-reducing bacterium desulfuromonas acetoxidans has been purified to homogeneity and characterized. it elicits absorption bands at 551.5, 522.5 and 418 nm in the reduced form; the absorptivity ratio aalpha(red)/a280nm(ox) equals 3.8 for the pure preparation. the molecular weight was estimated to be 9800 by gel filtration. determination of the amion acid composition and analysis of the n-terminal amino acid sequence showed the cytochrome to be identical wi ...1977192288
spectroscopic studies of the oxidation-reduction properties of three forms of ferredoxin from desulphovibrio gigas.electron paramagnetic resonance spectra were recorded of three forms of desulphovibrio gigas ferredoxin, fdi, fdi' and fdii. the g = 1.94 signal seen in dithionite-reduced samples is strong in fdi, weaker in fdi' and very small in fdii. the g = 2.02 signal in the oxidized proteins is weak in fdi and strongest in fdii. it is concluded that most of the 4fe-4s centres in fdi change between states c- and c2-; fdi' contain both types of centre. there is no evidence that any particular centre can chan ...1977189829
purification and some properties of cytochrome c553(550) isolated from desulfovibrio desulfuricans norway. 1979219858
purification and characterization of cytochrome c3, ferredoxin, and rubredoxin isolated from desulfovibrio desulfuricans norway.different electron carriers of the non-desulfoviridin-containing, sulfate-reducing bacterium desulfovibrio desulfuricans (norway strain) have been studied. two nonheme iron proteins, ferredoxin and rubredoxin, have been purified. this ferredoxin contains four atoms of non-heme iron and acid-labile sulfur and six residues of cysteine per molecule. its amino acid composition suggests that it is homologous with the other desulfovibrio ferredoxins. the rubredoxin is also an acidic protein of 6,000 m ...1977187570
the amino acid sequence of desulforedoxin, a new type of non heme iron protein from desulfovibrio gigas. 1979508323
hydrogenase activity in the dry state: isotope exchange and reversible oxidoreduction of cytochrome c3.hydrogenase (hydrogen:ferricytochrome c3 oxidoreductase, ec 1.12.2.1) catalyzes three types of reactions, i.e., (1) conversion between hydrogen modifications, para-h2 and ortho-h2, (2) exchange reaction between hydrogen isotopes, and (3) reversible oxidoreduction of an electron carrier with h2 and protons. we observed that purified desulfovibrio hydrogenase in the dry state could catalyze not only the conversion and exchange reactions (yagi, t., tsuda, m., mori, y. and inokuchi, h. (1969) j. am. ...1979222328
electrochemistry of cytochrome c3 from desulfovibrio desulphuricans (norway) [proceedings]. 1979225230
purification and properties of cytochrome c-553, an electron acceptor for formate dehydrogenase of desulfovibrio vulgaris, miyazaki.cytochrome c-553 of desulfovibrio vulgaris, miyazaki, was purified to homogeneity. the absorption spectrum of the ferro form has four peaks at 553, 525, 417 and 317 nm with a plateau near 280 nm, and that of the ferri form has three peaks at 525, 410 and 360 nm with a plateau near 280 nm and a shoulder at 560 nm. the millimolar absorbance coefficient of the alpha-peak of the ferro form is 23.9. the molecular weight of cytochrome c-553 is 8000, and it contains one heme. its isoelectric point is r ...1979226135
oxidation-reduction potentials of the hemes in cytochrome c3 from desulfovibrio gigas in the presence and absence of ferredoxin by epr spectroscopy.1. ferricytochrome c3 from d. gigas exhibits two low-spin ferric heme epr resonances with gz-values at 2.959 and 2.853. ferrocytochrome c3 is diamagnetic based on the absence of any epr signals. 2. epr potentiometric titrations result in the resolution of the two low-spin ferric heme resonances into two additional heme components representing in total the four hemes of the cytochrome, with em values of -235 mv and -315 mv at heme resonance i and em values of -235 mv and -306 mv at heme resonance ...1979227477
transformation of mercuric chloride and methylmercury by the rumen microflora.the microflora in strained rumen fluid did not methylate or volatilize 203hg2+ at detectable rates. however, there was an exponential decay in the concentration of added ch3hg+, which was attributed to demethylation. the major product of demethylation was metallic mercury (hg0), and it was released as a volatile product from the reaction mixture. demethylation occurred under both anaerobic and aerobic conditions. the rate of demethylation was proportional to the concentration of added ch3hg+-hg ...1979539820
epr spectroscopy of the iron-sulphur cluster and sirohaem in the dissimilatory sulphite reductase (desulphoviridin) from desulphovibrio gigas.desulphoviridin in the oxidized state showed epr signals around g = 6, consistent with the sirohaem being in the high-spin ferric state. this was unreactive with sulphite, sulphide or cyanide; but readily reduced by methyl viologen. when the enzyme was treated with na2s2o4 the sirohaem was slowly reduced and a spectrum of a reduced iron-sulphur cluster at g = 2.07, 1.93, 1.91 appeared over the course of an hour. an intermediate in this reaction was indicated by a free radical signal which appear ...1979228747
structure and sequence of the multihaem cytochrome c3.the molecular structure of cytochrome c3 isolated from desulfovibrio desulfuricans has been solved on the basis of its crystallographic determination at 2.5 a resolution and of an essentially complete sequence. the molecule consists of a single polypeptide chain wrapped around a very compact core of four non-parallel haems which present a relatively high degree of exposure to the solvent. alignment of the amino acid sequences of cytochrome c3 from several species suggests that the structure repo ...1979229424
cytochrome c3 from the sulfate-reducing anaerobe desulfovibrio africanus benghazi: purification and properties.cytochrome c3 was purified from desulfovibrio africanus benghazi by extraction with alkaline deoxyribonuclease, fractionation with ammonium sulfate, batch elution from carboxymethyl sephadex followed by chromatography on the same resin, and gel filtration on sephadex g-75. the preparation was judge homogeneous by a variety of criteria. the molecular weight was determined in an analytical ultracentrifuge, and values between 14,400 and 15,490 were obtained, depending upon the presumed value of par ...1979230179
on the redox properties of three bisulfite reductases from the sulfate-reducing bacteria. 1979230837
oxidative phosphorylation linked to the dissimilatory reduction of elemental sulphur by desulfovibrio.hydrogenase and cytochrome c3 purified from desulfovibrio gigas and d. desulfuricans strain norway form a soluble complex which is capable of transferring electrons from molecular hydrogen to colloidal sulphur (s0). in this reaction, sulphur is reduced to hydrogen sulphide. since both strains are capable of growth using elemental sulphur as terminal electron acceptor, it was of interest to check for oxidative phosphorylation in this sulphur reduction sytem. membranes isolated from d. gigas or d. ...1979232998
effect of enzymic assay conditions on sulfite reduction catalysed by desulfoviridin from desulfovibrio gigas.the type and the amount of end products resulting from sulfite reduction catalysed by a single partially purified desulfoviridin preparation from desulfovibrio gigas were shown to depend upon the enzymic assay conditions employed. both manometric and spectrophotometric assays were used, with reduced methyl viologen serving as the electron donor in each system. trithionate, thiosulfate, tetrathionate and sulfide were identified as possible end products. in the manometric assays, sulfide productio ...1975235295
some physical and kinetic properties of adenylyl sulfate reductase from desulfovibrio vulgaris.adenylyl sulfate reductase has been purified from the anaerobic sulfate-reducing bacterium, desulfovibrio vulgaris, and judged to be homogenous by several criteria. different forms of the enzyme could be visualized in polyacrylamide gels after electrophoresis and these polymeric species have been studied by a combination of absorption spectra, polyacrylamide gel electrophoresis, and sedimentation velocity experiments. a dimeric species of molecular weight 440,000 is stable in potassium phosphat ...1975235533
physicochemical properties of flavodoxin from desulfovibrio vulgaris.reductive titration curves of flavodoxin from desulfovibrio vulgaris displayed two one-electron steps. the redox potential e-2 for the couple oxidized flavodoxin/flavodoxin semiquinone was determined by direct titration with dithionite. e-2 was -149 plus or minus 3 mv (ph 7.78, 25 degrees c). the redox potential e-1 for the couple flavodoxin semiquinone/fully reduced flavodoxin was deduced from the equilibrium concentration of these species in the presence of hydrogenase and h-2. e-1 was -438 pl ...1975235984
a chemiosmotic model for sulphate respiration. 1978720591
dissimilatory reduction of bisulfite by desulfovibrio vulgaris.the reduction of bisulfite by desulfovibrio vulgaris was investigated. crude extracts reduced bisulfite to sulfide without the formation (detection) of any intermediates such as trithionate or thiosulfate. when the particulate fractions was removed from crude extracts by high-speed centrifugation, the soluble supernatant fraction reduced bisulfite sequentially to trithionate, thiosulfate, and sulfide. addition of particles or purified membranes to the soluble fraction restored the original activ ...1978721780
electron-transfer mechanisms in multihaem cytochromes [proceedings]. 1978744410
[formation and utilization of lactate during methane fermentation of cellulose].the role of lactate was studied in the course of methane formation from cellulose by a mixed bacterial association. lactate was accumulated in the medium during intensive degradation of cellulose and was assimilated in the period of active gas production. glucose added to the medium was converted into lactate within 30 min. if lactate served as a substrate for a methane forming association which was previously cultivated in a medium containing cellulose, it was almost completely converted into m ...1978745555
the n-terminal sequence of superoxide dismutase from the strict anaerobe desulfovibrio desulfuricans. 1977323056
rapid sampling culture chamber.an all-glass chamber for culturing anaerobic and aerobic bacteria in liquid medium is described. the system permits both rapid sampling and turbidimetric measurements under controlled atmospheric conditions.1978350159
radioisotope assay for the quantification of sulfate-reducing bacteria in sediment and water.a radioisotope enrichment culture method was developed to estimate the physiologically active component of a population of sulfate-reducing bacteria in environmental water and sediment samples. aliquots of water or sediment were added to 50-ml serum bottles filled with 35-s-sulfate broth incubated for approximately 30 h. after incubation, the disintegration rate per milliliter of spent medium was measured, and the percentage of loss of activity resulting from bacterial sulfate reduction was dete ...1975807162
the interaction of polymeric viologens with hydrogenases from desulfovibrio desulfuricans and clostridium pasteurianum.the interaction between hydrogenases from either desulfovibrio desulfuricans or clostridium pasteurianum and electron donors methyl viologen or polymeric viologens was examined. extracts from each organism contained a single gel electophoretic band of active hydrogenase. the hydrogenase of d. desulfuricans was much more stable than that of cl. pasteurianum. with methyl viologen apparent km and vm values were 0.5 mm and 0.62 mumole h2/min per milligram protein for the cl. pasteurianum and 0.7 and ...1979396014
amino acid sequence of desulfovibrio vulgaris flavodoxin.the complete amino acid sequence for the 148-amino acid flavodoxin from desulfovibrio vulgaris was determined to be: h3n+-met-pro-lys-ala-leu-ile-val-tyr-gly-ser-thr-thr-gly-asn-thr-glu-tyr-thr-ala-glu-thr-ile-ala-arg-glu-leu-ala-asn-ala-gly-tyr-glu-val-asp-ser-arg-asp-ala-ala-ser-val-glu-ala-gly-gly-leu-phe-glu-gly-phe-asp-leu-val-leu-leu-gly-cys-ser-thr-trp-gly-asp-asp-ser-ile-glu-leu-gln-asp-asp-phe-ile-pro-leu-phe-asp-ser-leu-glu-glu-thr-gly-ala-gln-gly-arg-lys-val-ala-cys-phe-gly-cys-gly-as ...1977402366
occurrence of glutathione in bacteria.glutathione and soluble thiol content were examined in a broad spectrum of bacteria. significant soluble thiol was present in all cases. the thiol compound was glutathione in most of the gram-negative bacteria but not in most of the gram-positive bacteria studied. glutathione was absent in four anerobes and one microaerophile but was present in a blue-green bacterium. the glutathione content of escherichia coli increased significantly during transition from exponential to stationary phase.1978417060
isolation and characterization of a molybdenum iron-sulfur protein from desulfovibrio africanus. 1979426817
significance and taxonomic value of iso and anteiso monoenoic fatty acids and branded beta-hydroxy acids in desulfovibrio desulfuricans.the fatty acids obtained from extractable lipids of the anaerobic sulfate bacterium desulfovibrio desulfuricans were identified. saturated and monoenoic iso (c15-c19) and anteiso (c15, c17) fatty acids and saturated normal (c14-c18) and monoenoic normal (c16, c18) fatty acids were shown to be shown to be present by capillary gas chromatography-mass spectrometry. iso and anteiso beta-hydroxy fatty acids were analyzed as trimethylsilyl ethers in the same way. the position of methyl branches in the ...1977845113
biochemical studies on sulfate-reducing bacteria. xv. separation and comparison of two forms of desulfoviridin.desulfoviridin from desulfovibrio vulgaris was separated into two forms by deae-sephadex column chromatography. the major form had a pi of 4.4 and the minor form one of 4.5-4.6. both forms produced mainly trithionate, besides thiosulfate and sulfide, in methylviologen-linked sulfite reduction. the specific activities of sulfite reduction, as well as of hydroxylamine reduction, were virtually identical in both forms. there were no great differences in their absorption spectra, cd spectra, molecul ...1979429261
anaerobic vibrio-like organisms cultured from blood: desulfovibrio desulfuricans and succinivibrio species.two unusual anaerobic vibrio-like organisms were recovered from blood cultures of two patients. one isolate was identified as desulfovibrio desulfuricans. it appeared to be the cause of a 24-h episode of fever, chills, and profuse perspiration. this is apparently the first documented report of human infection due to this organism. the second isolate was a succinivibrio species. it has rarely been described as a cause of bacteremia. the clinical significance of the organism remains unclear.1977858783
[participation of sulfate reducing bacteria in copper precipitation].chemical precipitation of copper by hydrogen sulphide at three values of ph (3.0; 5.0; 7.0) did not result in complete elimination of the metal from a solution. if sulphate reducing bacteria and a source of organic substance, for instance, disintegrated reed, are introduced into the medium, the microorganisms begin to grow, the redox potential decreases, hydrogen sulphide is formed, and copper is completely eliminated from a solution within 10--15 days in model experiments.1977859457
crystallographic data for cytochrome c3 from two strains of desulfovibrio vulgaris, miyazaki.two crystalline forms of cytochrome c3 isolated from two strains of desulfovibrio vulgaris, miyazaki, tentatively designated as d. vulgaris, miyazki f and d. vulgaris, miyazaki k, have been found. both belong to the orthorhombic system, space group p2(1)2(1)2(1), but have different cell dimensions; a=54.1, b=68.9 and c=35.0 a for d. vulgaris, miyazaki f, and a=43.5, b=41.2, and c=62.9 a for d. vulgaris, miyazaki k. the asymmetric unit of each crystal contains one molecule of cytochrome c3.1979479126
catalytic activity of the chromophore of desulfoviridin, sirohydrochlorin, in sulfite reduction in the presence of iron.sirohydrochlorin chromophore prepared by acetone/hcl treatment of desulfoviridin, the sulfite reductase from desulfovibrio, catalyzed the reduction of sulfite to sulfide and thiosulfate in equimolar amounts when coupled with a hydrogen-hydrogenase-methyl viologen system. this activity was manifested at acidic ph and increased exponentially with decrease in ph. the km value for sulfite was nearly 10 times that of desulfoviridin. inorganic iron was necessary for the reduction, since inactivation o ...1979479127
solar-driven chemical energy source for a martian biota.microorganisms deep in the martian soil could derive energy indirectly from the sun via chemical reactions involving atmospheric products of the solar ultraviolet flux. the viking discovery of a chemically uniform regolith which, though poor in organics, is rich in sulfur-containing compounds suggests reaction sequences in which sulfur is recycled through reduced and oxidized states by biologically catalyzed reactions with photochemically-produced atmospheric constituents. one candidate reaction ...1979481874
isolation and characterization of a rubredoxin and two ferredoxins from desulfovibrio africanus.rubredoxin and two distinct ferredoxins have been purified from desulfovibrio africanus. the rubredoxin has a molecular weight of 6000 while the ferredoxins appear to be dimers of identical subunits of approximately 6000 to 7000 molecular weight. rubredoxin contains one iron atom, no acid-labile sulfide and four cysteine residues per molecule. its absorbance ratio a278/a490 is 2.23 and its amino acid composition is characterized by the absence of leucine and a preponderance of acidic amino acids ...1979508735
amino acid sequence of desulfovibrio gigas ferredoxin: revisions. 1979518659
[distribution of thermophilic sulfate reducing bacteria in the oil-bearing strata of apsheron and western siberia].enrichment cultures of sulfate-reducing bacteria were obtained by inoculating the water of high-temperature gas and oil-bearing strata. a study of the morphology of these cells has shown that the thermophilic bacterium desulfovibrio thermophilus occurs in the deposits of the apsheron peninsula while desulfootomaculum nigrificans is found in the deposits of west siberia. the former organism is involved in the accumulation of h2s in the apsheron strata. the temperature of the strata is believed to ...1979530138
sulfide production by some rumen bacteria. 1979533168
the discovery of nature's biosynthetic pathways.uro'gen-iii is a key intermediate on the biosynthetic pathways to the vitally important natural pigments haem, chlorophyll and the cytochromes. how the unexpected structure of uro'gen-iii is synthesized by living things has long been a major puzzle. studies based on 13c-labelling are described which show a) that a single intramolecular rearrangement occurs and b) that this step occurs after the open-chain linear tetrapyrrole system has been built. a second study involves stereospecific labelling ...1978620714
separation of hydrogenase from intact cells of desulfovibrio vulgaris. purification and properties. 1978620819
isolation and characterization of desulfovibrio growing on hydrogen plus sulfate as the sole energy source. 1978623496
the taxonomy of some new isolates of dissimilatory sulfate-reducing bacteria.the interrelationships between 92 isolates of sporing and non-sporing sulfate-reducing bacteria were determined. of the 116 biochemical and physiological characteristics examined, only 25 were useful for discrimination of groups. responses to most of the test were negative. a similarity coefficient and a principal component factor analysis of these data were made. the deoxyribonucleic acids buoyant densities (dna) from all strains and the electrophoretic properties of adenylylsulfotransferase (a ...1977922597
structural control of the redox potentials and of the physiological activity by oligomerization of ferredoxin. 1978658398
the role of dihydrogen and hydrogenase in nitrogen fixation. 1978667177
reactivity of desulfovibrio gigas hydrogenase toward artificial and natural electron donors or acceptors.a purified preparation of hydrogenase from d. gigas was inactive toward ferredoxin, flavodoxin or rubredoxin in the absence of cytochrome c3 (m.w. 13,000), in an atmosphere of hydrogen, although direct reduction of benzyl viologen or fmn was possible. the hydrogen evolution reaction from dithionite was possible with methyl viologen. the same reaction also occured with cytochrome c3 (m.w. 13,000) or cytochrome c3 (m.w. 26,000). addition of either ferredoxin or flavodoxin did not accelerate the re ...1978667185
inhibition of methanogenesis by sulphate reducing bacteria competing for transferred hydrogen.a methanogenic bacterial consortium was obtained after inoculation of benzoate medium under n2/co2 atmosphere with intertidal sediment. a hydrogen donating organotroph and methanococcus mazei were isolated from this enrichment. h2-utilising sulphate reducing bacteria were isolated under h2/co2 in the absence of organic electron donors. the methanococcus was able to produce methane in yeast extract medium under n2/co2 if the h2 donating organism was present, and sulphate reduction occurred if the ...1978678014
hydrogen as a substrate for methanogenesis and sulphate reduction in anaerobic saltmarsh sediment.hydrogen gas stimulated sulphate reduction in a saltmarsh sediment and the importance of h2 transferred from organotrophic bacteria to the sulphate-reducers is discussed. beta-fluorolactate inhibited sulphate reduction whether lactate, ethanol or hydrogen was being used as growth substrate. when added to sediment beta-fluorolactate inhibited sulphate reduction with a consequent increase in methane production. addition of h2 stimulated methanogenesis in sediment and this stimulation was greater i ...1978678015
effect of the concentration of available carbon compounds on the microbial reduction of sulphates.a change of the concentration of available carbon compounds in the reaction medium affecting the carbon to sulphur ratio (c/s) affects the reduction degree of sulphates. the time interval between the initial and the log phase of the process (to) also varies depending on the concentration of carbon compounds. it was demonstrated that optimal reduction conditions for the strain desulfovibrio desulphuricans used exist in media of c/s = 1.84 +/- 0.2. kinetic data for the reduction process are presen ...1975241212
purification and properties of thiosulfate reductase from desulfovibrio gigas.thiosulfate reductase of the dissimilatory sulfate-reducing bacterium desulfovibrio gigas has been purified 415-fold and its properties investigated. the enzyme was unstable during the different steps of purification as well as during storage at - 15 degrees c. the molecular weight of thiosulfate reductase estimated from the chromatographic behaviour of the enzyme on sephadex g-200 was close to 220000. the absorption spectrum of the purified enzyme exhibited a protein peak at 278 nm without char ...1975242299
[mixed cultures of heterotrophic, sulfate-reducing and sulfur phototrophic bacteria (author's transl)].in mixed cultures, carbon and electron sources for desulfovibrio desulfuricans are excreted by escherichia coli from glucose fermentation. desulfovibrio produces substrates for chlorobium strain.1976791172
metal accumulation by bacteria with particular reference to dissimilatory sulphate-reducing bacteria.dissimilatory sulphate-reducing bacteria, genera desulfovibrio and desulfotomaculum, exhibit a superior ability, over assimilatory organisms, to extract three amounts of metals from culture media. this property does not appear to be solely a function of the presence of h2s. in media containing elevated amounts of fe, electron dense particles, provisionally identified as fes, are deposited within the cells of dissimilatory bacteria.1976824869
[mutual relations between plastic materials and bacteria (author's transl)].the complex problems of microbiological degradation of synthetic plastics and a fairly wide variety of 62 testing materials, belonging to 14 major groups of plastics, are described. adaequate and reliable testing techniques had to be devised. drawing on the experiences of h. braun, 1930, and of bushnell and haas, 1941, as to the metabolism of bacteria and the utilization of certain hydrocarbons by microorganisms, and previous research work by a. schwartz in berlin, 1959-60, on microbial corrosio ...1976828369
nmr characterization of three forms of ferredoxin from desulphovibrio gigas, a sulphate reducer.a nmr and magnetic susceptibility study of the oxidized and reduced states of three different oligomers (forms) of a [4fe-4s] ferrodoxin protein from desulphovibrio gigas, fdi, fdi', and fdii was carried out. fdi and fdi' are different trimers and fdii a tetramer of the same basic subunit. a probable assignment of the contact shifted resonances is indicated. since the temperature dependences of the contact shifted responances associated with each [4fe-4s] are not all similar a delocalized model ...1977836818
growth of sulfate-reducing bacteria with sulfur as electron acceptor.in addition to three new isolates, six strains of representative species of sulfate-reducing bacteria were tested for their capacity to use elemental sulfur as an electron acceptor for growth. there was good growth and sulfide production by strain norway 4 and the three isolates, two of which had been enriched with sulfur flower and one isolated from a culture with green sulfur bacteria. slow but definite growth was observed with desulfovibrio gigas. the type of strains of desulfovibrio desulfur ...1977843165
an iron-containing superoxide dismutase from the strict anaerobe desulfovibrio desulfuricans (norway 4).superoxide dismutase, the enzyme which catalyzes the dismutation of superoxide free radicals (formula: see text) has been purified to homogeneity from the strict anaerobe sulfate-reducing bacterium desulfovibrio desulfuricans (norway 4). its molecular weight is 43,000 and it is composed of two subunits of equal size which are not covalently bound. the enzyme was found to contain iron by atomic absorption and the absence of acid-labile sulfur indicates that it is not an iron-sulfur protein. elect ...1977857918
isolation and characterization of desulforedoxin, a new type of non-heme iron protein from desulfovibrio gigas. 1977861023
fractionation of sulfur isotopes by continuous cultures of desulfovibrio desulfuricans.sulfur isotope effects observed in lactate-limited continuous cultures of desulfovibrio desulfuricans were, in general, similar to those reported for sulfate reduction by washed cells and batch cultures. there was a trend towards higher fractionation at low growth rates.19751201506
phylogenetic studies of two rubredoxins from sulfate reducing bacteria.the sequences of two rubredoxins isolated from the sulfate reducing bacteria: desulfovibrio vulgaris and desulfovibrio gigas have been elucidated. they have similar sequences but many more differences occur than would be expected from two bacteria of the same genus. of the 52 sites, only 37 are occupied by identical residues. the primary structures are compared with those of the anaerobic bacteria rubredoxins of clostridium pasteurianum, micrococcus aerogenes, pseudomonas oleovorans and peptostr ...1977864718
atp and acetylene-reducing activity of a sulfate-reducing bacterium.a sulfate-reducing bacterium, a strain of desulfovibrio vulgaris, when lactate or pyruvate was supplied as an electron and energy source, reduced acetylene only in the presence of sulfate. acetylene reduction started after a lag of 1 h. h2 which acts as an electron and energy source for sulfate reduction was unable to reduce acetylene even in the presence of sulfate. it was suggested, from the measurements of the amounts of adenine nucleotides of the bacterium incubated under various conditions, ...1977871967
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