calf liver nuclear n-acetyltransferases. purification and properties of two enzymes with both spermidine acetyltransferase and histone acetyltransferase activities.calf liver contains two nuclear n-acetyltransferases which are separated by chromatography on hydroxylapatite. both acetyltransferase a and acetyltransferase b will transfer acetate from acetyl-coa to either histone or spermidine. the same protein catalyzes the reaction with both substrates; this is shown by a constant ratio of spermidine to histone activity over a 5,000-fold purification and identical heat denaturation kinetics for both spermidine and histone acetyltransferase activity with eac ...197822543
purification of pili from bacteroides nodosus and an examination of their chemical, physical and serological properties.pili from bacteroides nodosus were purified to greater than 99% homogeneity by precipitation at ph 4.0 and in mgcl2 followed by chromatography on biogel a150. the pili were composed entirely of one type of polypeptide subunit, pilin. no carbohydrates, nucleic acid, lipid, lipopolysaccharide or phosphate could be detected in purified pili preparations. the molecular weight of pilin from b. nodosus strains 91b and 198 was 18,400 and from strain 80 was 19,300. the isoelectric points of pili from b. ...197943354
studies on the antigenic structure of bacteroides nodosus.the antigenic mosaics of three bacteroides nodosus isolates (198, 199 and 127) were studied to elucidate the nature of the protective immunogen. in vaccinated sheep the three isolates induced high homologous serum agglutinin titres but it was also apparent that 198 and 199 shared a major surface antigen not present on 217. this major cross-reacting antigen was not detected with rabbit antisera. the fimbriae, consisting predominantly of protein, induced high homologous titres in rabbits and repre ...197879200
rat liver cytosolic azoreductase. electron transport properties and the mechanism of dicumarol inhibition of the purified enzyme. 197991608
triiodothyronine (t3) receptors in brain and liver during induced hypothyroidism in rats [proceedings]. 197992254
[physicochemical properties of neurospecific basic protein (scp) of the spinal cord].basic protein (scp) known as antiencephalytogenic was isolated from the bull spinal cord and spinal roots. the protein is purified to a molecular homogeneous state, some of its physicochemical properties are studied. molecular homogeneity of the protein is established by the method of disk electrophoresis in 15% polyacrylamide gel with sodium dodecyl sulphate present and by analytic ultracentrifugation. during electrophoresis in 15% polyacrylamide gel at ph 4.0 the protein movement occurs only i ...197992837
the effect of dose loading and of double labelling with 57co and 125i on the tissue distribution in animals.dose loading effects upon the performance of 57co-bleomycin as a tumor localizing agent have been investigated in rhabdomyosarcoma bearing wag/ry rats. the addition of non-radioactively labelled co-bleomycin increased the relative uptake of 57co-bleomycin in rapid growing tumors, but the addition of non-chelated bleomycin had no influence at all. in our experimental system, iodinated bleomycin generally labelled by reaction with icl, was found to be an unsatisfactory tumor localizing agent. in o ...197993048
stimulatory effect of dopamine receptor agonists on prolactin secretion by cultured rat pituitary cells [proceedings]. 197993913
presence of the asialoglycoproteins-binding protein in primary monolayer culture of adult rat hepatocytes [proceedings]. 197993914
effects of subminimal inhibitory concentrations of ampicillin, chloramphenicol, and nitrofurantoin on the attachment of escherichia coli to human uroepithelial cells in in vitro system with epithelial cells of the human urinary tract and escherichia coli isolated from the urine of pateints with acute pyelonephritis was used for tests of the effect of antibacterial agents on bacterial adhesion. e. coli treated with subminimal inhibitory amounts of ampicillin attached less than untreated control bacteria. subminimal inhibitory amounts of chloramphenicol and nitrofurantoin had no effect on adhesion of the 15 strains of e. coli tested. the combination of bacteri ...197994452
a rat model (using a semipurified diet) of the fetal alcohol syndrome.a semipurified liquid diet was used to develop a chronic rat model of the fetal alcohol syndrome. control female rats gained weight normally, reproduced normally, and gave birth to normal litters on this diet. increased neonatal mortality, decreased neonatal weights, and altered sex ratios were observed in offspring of experimental alcoholic animals. preliminary histological results include a mild delay in cell lamination patterns in the cerebral cortex at 4 days postnatal in alcoholic offspring ...197995338
intestinal disaccharidases in newborn and mother rats: effect of dietary protein deficiency during pregnancy. 197896687
phycobiliprotein synthesis in protoplasts of the unicellular cyanophyte, anacystis nidulans.stable and metabolically active protoplasts were prepared from the unicellular cyanophyte, anacystis nidulans, by enzymatic digestion of the cell wall with 0.1% lysozyme. the yield of protoplasts from intact algal cells was approx. 50%. incorporation of l-[u-14c]leucine into cold trichloroacetic acid-insoluble material from protoplasts preparations was linear for 1.5 h and continued for an additional 2.5 h. incorporation of radiolabeled leucine into hot trichloroacetic acid-insoluble material fr ...197896857
leukocytic endogenous mediator. 197897380
an analysis of group d streptococci recovered from cancer patients. 197898452
bacillus of calmette and guérin and 7, 12-dimethylbenz(a)-anthracene carcinogenesis in mice.the effects of single or repeated bacillus of calmette and guérin (bcg) treatment on the onset of tumors induced by the carcinogen 7,12-dimethylbenz(a)anthracene (dmba) injected at birth into swiss mice were studied. multiple doses of bcg, given at regular intervals significantly lowered the incidence of carcinogen-induced tumors. the dmba-induced lung adenomas did not differ morphologically from the ones that appeared in mice simultaneously treated with both dmba and bcg.197898879
[immunological relations between mother and child during pregnancy (author's transl)].the relations between the maternal and foetal organisms during pregnancy are partly very complicated and remain inexplicable. blastocyst, trophoblast, embryo and foetus have membrane antigens at a very early stage, and the immunocompetence of the foetus develops already during the second third of the pregnancy period. many non-specific factors are involved in preventing the castingoff reaction of the foetal organism. the trophoblastic tissue is probably of central importance. the focal point of ...197898927
[some aspects relating to the aflatoxin generation during the self-heating of cereals].in a stored batch of grain which was already affected by mould-formation tests were carried out with the known aflatoxin producer aspergillus flavus. a significantly lower aflatoxin production ensued if the mould growth was not connected with self-heating of the stored product. however, in conformity with increasing mould formation the germinating power was adversely affected and the significant signs (fatty acid number, reductive and none-reductive sugars) were influenced in the grain, irreleve ...197898933
age-associated changes in the blood picture of the goat. 197898936
restraint-induced inhibition of 7,12-dimethylbenz[a]anthracene-induced mammary tumors: relation to stages of tumor development.experiments on the use of chronically applied restraint to reduce the number of mammary tumors developing in response to treatment of rats with 7,12-dimethylbenz[a]anthracene indicated that this inhibitory effect was largely due to restraint applied after the induction period; preinduction restraint and induction period restraint had no significant effect on tumor development. after termination of the restraint treatment, the rate at which new tumors appeared first increased and then decreased. ...197899522
[volatile fatty acids in gastrointestinal diseases in young infants]. 1978101958
structural determination of 'cord factor' from a corynebacterium diphtheriae strain by a combination of mass spectral ionization methods: field desorption cesium cationization and electron impact mass spectrometry studies.the composition and structure of a preparation of 'cord factor' (di-beta-hydroxy acyl trehaloses) from corynebacterium diphtheriae have been determined by a combination mass spectral ionization methods. the methods were tested by means of synthetic 6,6'-dicorynomycolate of alpha-d-trehalose prior to their use on natural products. the determination of the molecular weight of the components and the estimation of their relative abundance in the natural mixture were made by field desorption mass spe ...1978106902
acetylated methylmannose polysaccharide of streptomyces griseus. locations of the acetyl groups.the positions of esterification of the 4 to 5 acetyl residues in the acetylated methylmannose-containing polysaccharide from streptomyces griseus have been established by the methyl replacement technique, wherein ester substituents are specifically replaced with methyl ether substituents. the newly incorporated methyl groups were distinguished from 3-o-methyl groups by the use of polysaccharide containing radioactively labeled endogenous methyl groups. the positions of methyl group localization ...1979107171
sarcocystinae: nomina dubia and available names.examination of the original descriptions of the species of sarcocystis in cattle, sheep, and swine, and of isosporid oocysts shed sporulated by dogs, cats, man, and other carnivores, has shown that it is not possible in most instances to identify unambiguously recently recognized taxa. the original descriptions are insufficient, and because no type specimens exist, could apply to two or more of the presently recognized taxa. we consider the following nomina dubia: sarcocystis hirsuta s. miescher ...1979107666
[lactic acid bacteria and streptococci of the human stomach normally and in pathological processes].lactic acid bacteria were either absent or present in negligent quantities in the stomach of apparently healthy man. but in case of various pathological processes in the stomach and duodenum lactic acid bacteria accumulated (up to a million cells per 1 ml of gastric contents). under these conditions coccal forms sharply prevailed over the bacillary ones. in patients with chronic gastritis and peptic ulcer of the stomach the most frequent were streptococcus faecalis and its variants; in oncologic ...1979107685
patterns of food intake in the monkey during free or restricted feeding and in response to 2-dg or insulin injection. 1979111267
arthrosonography: gray-scale ultrasound evaluation of the shoulder.gray-scale ultrasound images of the shoulders of 6 rhesus monkeys were obtained before and after fluid instillation to assess the ability of ultrasound to diagnose joint disorders. the normal bony landmarks and muscular structures were shown. after the fluid was introduced, fluid collections were identified in the axillary pouch and subscapular bursa. ultrasound is a promising new method of detecting intra-articular effusions of the shoulder.1979111302
cell-mediated immune reactions in vitro to cell walls and peptidoglycan from staphylococcus aureus.migration inhibition factor (mif) was produced by peritoneal exudate (pe) cells from guinea pigs sensitized with heat-killed cells of staphylococcus aureus cultured in vitro with staphylococcal cell walls or defined subunits of the cell walls. mif activity was assayed by inhibition of migration of alveolar macrophages from lungs of normal guinea pigs. specificity of inhibition was established using pe cells from tuberculin sensitive guinea pigs and tuberculin as appropriate controls. staphylococ ...1975126563
surface properties and in vitro functional capacities of human t lymphocyte subsets separated by linear density gradients.human lymphocytes derived from various central and peripheral sources were separated on linear density gradients (ldg). cells from individual density fractions were tested in parallel for: the capacity to form nonimmune rosettes with neuraminidase-treated srbc, the number of surface-associated htla, and in vitro proliferative responses to mitogenic lectins and alloantigens. heterogeneous density distribution profiles were obtained for all sources of human t cells and revealed an organ specificit ...1979156234
kinetics of glycerol uptake by the perfused rat liver. membrane transport, phosphorylation and effect on nad redox level.the kinetics of glycerol uptake by the perfused rat liver were determined according to a model which includes membrane transport, intracellular phosphorylation and competitive inhibition of glycerol phosphorylation by l-glycerol 3-phosphate. the membrane transport obeys first-order kinetics at concentrations below 10 mm in the affluent medium. the k-m of the glycerol phosphorylation was 10 mum and the k-i of the l-glycerol 3-phosphate inhibition was 50 mum. the maximum activity (v) was 3.70 mum ...1975164217
colchicine resistance in mammalian cell lines.colchicine-resistant variants derived from mouse and syrian hamster lines are described. the resistant cells do not appear to be true mutants, since they appear at a high frequency, unaffected by treatment with ethyl methyl sulphonate, and are unstable in the absence of the drug. they are cross-resistant to other drugs, show a reduced rate of binding of colchicine in monolayer, and give extracts with colchicine-binding properties identical to those of the wild type. thus the resistance is due to ...1975165207
influence of age and sex on carcinoma and cirrhosis of the liver in axc strain rats ingesting 0.025% n-2-fluorenyldiacetamide.inbred a x c male and female rats, 4, 12, 24, and 52 weeks of age, ingested 0.025% n-2-fluorenyldiacetamide in a semisynthetic diet. both age and sex were important in the development of hepatic lesions. the 4- and 12-week-old males developed a higher incidence of carcinomas and cirrhosis of the liver than did the females of the same ages or the males and females 24 and 52 weeks of age. four-week-old male rats had more carcinomas per liver, larger carcinomas, more poorly differentiated (as compa ...1975179045
the effect of induced hyperglucagonaemia on the zucker fatty rat.the effect of chronic treatment with a long-acting glucagon preparation on liver glucagon and insulin receptors, adenylate cyclase and plasma lipids has been examined in zucker fatty rats (fa/fa) and their lean littermates (fa/-). liver insulin and glucagon receptors were examined using radioreceptor assay techniques. neither fatty nor lean rats showed any change in insulin receptors after glucagon treatment. glucagon receptors of the fatty rats showed a 33% drop in the number of the glucagon re ...1978208903
[functional organization of papova- and adenovirus dna]. 1978212867
the cyclic 3',5'-adenosine monophosphate receptor protein and regulation of cyclic 3',5'-adenosine monophosphate synthesis in escherichia coli.rates of synthesis of cyclic 3',5'-adenosine monophosphate (camp) were measured in cultures of escherichia coli aerating without a carbon source. this technique provides a representative measure of adenylate cyclase activity in the absence of inhibition caused by transport of the carbon source. adenylate cyclase activity was found to vary more than 20-fold depending on the carbon source that had been available during growth. synthesis of camp in cells aerating in the absence of the carbon source ...1978213702
the effect of prostaglandins f2alpha and e2 on rat brain n-acetyl-beta-d-glucosaminidase in vitro. 1978214799
[antiviral activity of phenolic polymers against type 1 herpesvirus hominis]. 1978214800
proliferation of rous sarcoma virus-infected, but not of normal, chicken fibroblasts in a medium of reduced calcium and magnesium concentration.both normal and rous sarcoma virus-infected chicken fibroblasts proliferate actively in a culture medium containing physiological concentrations of calcium (1.2 mm) and magnesium (0.7 mm). in the presence of a physiological concentration of magnesium, reduction of the calcium concentration to 0.125 mm resulted in a significant decrease in the proliferation of the normal, but not of the neoplastic, fibroblasts. reduction of the magnesium concentration to 0.05 mm in the presence of a physiological ...1979226989
studies on the ultrastructural morphology of bacteroides nodosus.the morphology of bacteroides nodosus was examined with the electron microscope. b nodosus stained with solium phosphotungstate and uranyl acetate possessed fimbriae and in addition organisms negatively stained with sodium phosphotungstate often possessed rings on their surface. phage-like particles were also observed in negatively stained preparations. in thin sections, b nodosus had a multilayered cell envelope and the type of cell division characteristic of gram-negative bacteria. the cytopla ...1979262607
differences in morphology of bacteroides nodosus attributable to culture media. 1977275674
blockage of narcotic-induced dopamine receptor supersensitivity by cyclo(leu-gly).we have previously reported that the administration of cyclo(leu-gly) to mice prior to morphinization blocked the development of tolerance to the analgesic effects of morphine as well as the development of some signs of physical dependence. in the present series of experiments, the effect of the same peptide treatment on changes in dopamine receptor sensitivity induced by chronic morphine treatment were determined. changes in dopamine receptor sensitivity were determined by measuring (i) the eff ...1979293696
[determination of the localization of the kcpa gene on the chromosome of e. coli o124. i. use of the type f' plasmids of varying length].the loss of virulence was observed in some of the transconjugates of e. coli o124 in the process of the transfer of the plasmids differing in length: f' 200 ps, f'x 573 and f'x 363, carrying the genes of the chromosome area lac--pur e. the genetic determinants contributing to the ability of e. coli o124 to produce keratoconjunctivitis seem to be localized near the lactose marker, and not near e as in sh. flexneri. in e. coli o124 these genetic determinants are probably localized on both sides of ...1979373340
method of production of and antibody response to an oil adjuvant bacteroides nodosus bacterin.a procedure is described for the production of a highly concentrated bacteroides nodosus bacterin. an oil adjuvant bacterin which elicited the best antibody response in rabbits produced a response in sheep which lasted for at least 6 months.1978375151
the association of var1 with the 38 s mitochondrial ribosomal subunit in yeast. 1979387793
effect of age, weight and adequacy of zinc intake on the balance between alkaline ribonuclease and ribonuclease inhibitor in various tissues of the rat.1. deficiency of zinc inhibits growth and also increases the activity of alkaline ribonuclease in certain tissues of the rat (prasad & oberleas, 1973). zn could influence ribonuclease activity by direct effects on the enzyme or its natural inhibitor, or non-specifically as occurs when growth rate is affected by various other factors. these possibilities were studied. 2. alkaline ribonuclease was shown to be inhibited by zn in vitro, but the concentrations of zn required were so high that the enz ...1978415757
a degrading proteinase test to distinguish benign and virulent ovine isolates of bacteroides nodosus.bacteroides nodosus was recovered from naturally occurring cases of virulent ovine footrot (vfr) and benign footrot (bfr) using an artificial culture medium which incorporated trypticase, arginine, serine and 5% agar. a degrading proteinase (dp) test was developed which measured the proteinase activity of broth cultures of b. nodosus for a period of time after organism death to assess the stability of the enzyme. the spectrophotometric measurement of the release of dye from a hide powder--azure ...1979435206
comparison of oil adjuvant and alum precipitated bacteroides nodosus vaccines in treatment of foot-rot. 1979462727
the role of elastase in the differentiation of bacteroides nodosus infections in sheep and cattle.eighty-seven bacteroides nodosus isolates were examined for elastase production by clearing of elastin particles in tas agar medium. these included 54 ovine virulent isolates, 28 ovine benign isolates and five bovine isolates. in addition 22 ovine virulent, 16 ovine benign and two bovine isolates were examined for decline in proteolytic activity over a 13-day period in the degrading proteinase test using hide power-azure as substrate. there was a remarkable correlation between elastase productio ...1979504813
treatment of ovine foot-rot by vaccination with the specific aetiological agent bacteroides nodosus. 1979544167
igg1 and igg2 immunoglobulins to bacteroides (fusiformis) nodosus protease in infected and immunized sheep.sheep experimentally infected with foot rot developed antibodies that neutralized the proteolytic activity of bacteroides nodosus culture filtrates in vitro. similar antibody activity developed in sheep vaccinated against foot rot. in both cases the antibody was initially only associated with the igg2 fraction of serum, but with time there was an increase in the proportion associated with igg1. a subsidiary protein peak devoid of antiprotease activity was observed in elution profiles of affected ...1978569644
biochemical and biological studies on the lipopolysaccharide of bacteroides nodosus.lipopolysaccharides (lps) were extracted from three bacteroides nodosus isolates by the aqueous phenol method and purified by ultracentrifugation. the structure of b nodosus lps appears to be similar to enterobacterial lps, with the polysaccharide component joined to the lipid a moiety through an acid-labile 2-keto-3-deoxyoctonic acid (kdo) linkage. b nodosus lps contained glucose, galactose, hexosamine, kdo and heptose and no sugars apart from ribose, which was possibly a nucleic acid contamina ...1977605298
the role of various antigenic fractions of bacteroides nodosus in eliciting protection against foot-rot in vaccinated sheep.sheep were vaccinated against foot-rot with various antigenic extracts of bacteroides nodosus and challenged to assess their resistance. the protective immunogen was shown to be a heat-labile, high molecular weight surface antigen which was removed by ultrafiltration and was either identical to or closely associated with the fimbriae of the organism. resistance appeared to be associated with the presence of a single high molecular precipitinogen distinct from the 0 antigen. agglutinating antibod ...1978625599
the occurrence, prevalence and transmission of bacteroides nodosus infection in cattle.following reports of findings of ovine foot-rot flora in the feet of cattle, a study was undertaken to determine the prevalence of bacteroides nodosus infection in the apparently normal cattle population. we found that 34.5 to 74.2 per cent of the animals examined on different farms had b nodosus present in one or more feet. b nodosus was not the most prevalent bacterium observed in smears from cattle. other gram negative species including fusiformis necrophorus and many gram positive cocci and ...1978674842
recognition in great britain of bacteroides nodosus in foot lesions of cattle. 1977878236
selective medium for isolation of bacteroides nodosus.the sensitivity of bacteroides nodosus, the causative agent of sheep foot rot, to 24 selected antimicrobial agents was tested. many contaminants ordinarily associated with foot rot lesions were sensitive to lincomycin, whereas b. nodosus demonstrated resistance to this antibiotic. a concentration of 1 microgram of lincomycin per ml of basal medium optimally inhibited contaminants while allowing growth of b. nodosus. the basal medium was eugon agar with 0.2% yeast extract and 10.0% defibrinated h ...1977908746
determination of some in vitro growth requirements of bacteroides nodosus.physical and nutritional factors required for growth of bacteroides nodosus isolates from ovine foot-rot lesions were examined. simplified anaerobic culture techniques were devised utilizing a fully soluble, autoclavable, liquid medium (tas) which contained proteose-peptone, yeast and meat extracts and certain other essential compounds required to promote prompt and serially transferrable growth of cultures from small inocula. the latter included trypticase, arginine, a reducing agent (most suit ...19751133574
effects of prostaglandins e-1 and f-2alpha on serum luteinizing hormone concentration and on some sexual functions in male rabbits.pge-1(50 mu-g/animal) and pgf-2alpha (250 mu-g/animal) caused a transient increase in serum lh at 5 min after injection. pge-1(250 mu-g/animal) had a biphasic effect on serum lh. a small peak was obtained at 5 min, and a second, larger peak at 60 min after injection. it is suggested that the first peak is a result of the stress associated with injection of the pgs, whereas the second peak represents a physiological effect of pge. subcutaneous injection of pge-1(1 mg in arachis oil b.i.d.) for 10 ...19751138302
biochemistry of trichinella spiralis. 19751189439
an association between fimbriae and pores in the wall of fusiformis nodosus. 19751206378
a device for continuous intravenous fluid injections in dogs. 19761263430
[effect of tricyclic antidepressive agents on hemodynamics and plasms norepinephrine level in man during rest and exertion].the effects of i.v. injected antidepressants imipramine (tofranil, geigy) and dimetacrine (istonil, siegfried) on cardiavascular functions were investigated in 18 patients at diagnostic cardiac catheterization. 4 patients were additionally studied during ergometric exercise before and 45 min. after application of both thymoleptica. determinations of noradrenaline plasma concentrations were carried out in 8 patients. the results show of 25 mg (= 22,1 mg free base) imipramine or 33.5 mg (= 21.4 mg ...19761266277
humoral responses to a multivalent vaccine in age-matched lambs of different bodyweight and nutrition.k-agglutination, pilus-enzyme-linked immunosorbent assay (elisa) and outer membrane protein-elisas were used to assess humoral responses after vaccination with a commercial, multivalent, ovine foot rot vaccine (dichelobacter nodosus whole cells) in three groups of nine-month-old lambs of markedly different bodyweight, nutritional history and dietary protein supply. mean bodyweights of lambs in low (l), medium (m) and high (h) bodyweight/nutrition groups were 22, 32 and 48 kg, respectively, at th ...19921352408
molecular characterization of a genomic region associated with virulence in dichelobacter nodosus.the major pathogen implicated in footrot, a highly contagious disease of sheep, is the strict anaerobe dichelobacter nodosus (formerly bacteroides nodosus). sequence analysis of a 2,262-bp segment of the d. nodosus genome which is more prevalent in virulent isolates than in other isolates showed the presence of four open reading frames which appeared to have consensus transcriptional and translational start signals. these virulence-associated genes have been designated vapabcd. two of the three ...19921398971
amino acid and dna sequences of an extracellular basic protease of dichelobacter nodosus show that it is a member of the subtilisin family of proteases.a dna fragment encoding an extracellular basic protease (pi approximately 9.5) from dichelobacter nodosus, a gram-negative obligate anaerobe and the causative agent of ovine footrot, has been cloned and expressed in escherichia coli and sequenced. e. coli harbouring a plasmid with a 3-kb dna fragment containing the d. nodosus basic-protease gene exhibited proteolytic activity when tested on skim-milk plates. the sequence of the native basic protease isolated from d. nodosus was also determined b ...19921446666
colostral transfer of bacteroides nodosus antibodies in sheep.ovine contagious foot rot may cause lameness in sheep, resulting in decreased wool growth and low weight gain. affected neonatal lambs are difficult to treat, and treatment is labor intensive; thus, a method of prevention is warranted. vaccination of ewes with a multivalent vaccine in an oil adjuvant induced development of antibody to the somatic o antigen of bacteroides nodosus, and this antibody was detected in serum of newborn lambs after consumption of colostrum from the vaccinated ewes. ant ...19921506249
evaluation of a simple method for improving the precision of an elisa detecting antibody in serum.a method of correcting od values to improve between-assay precision (pcf4 method) was evaluated using an elisa detecting anti-dichelobacter nodosus antibody in ovine serum. four control sera with pre-defined target od were included on each plate and used to calculate a correction factor. the mean between-plate cv for eight reference sera tested over a period of 6 months was reduced from 15.2% to 9.4% in a polyclonal conjugate assay (114 plates) and from 13.3% to 9.8% in a monoclonal conjugate as ...19921564335
microbiological study of foot-rot in lambs: isolation, elastolytic activity and antimicrobial susceptibility.a microbiological study was made of an outbreak of foot-rot in lambs. elastolytic activity was detected in all 10 bacteroides nodosus strains isolated from the hooves of 9 affected animals. among 14 other isolated strictly anaerobic microorganisms belonging to different species or genera only 4 strains showed a low degree of elastolysis. the 14 antimicrobial agents studied effectively inhibited "in vitro" growth of b. nodosus.19921642074
[the influence of acth and hydrocortisone on drug kinetics in calves in the example of the antipyrine test].the aim of the experiment carried out on 2 groups (to comprise 8 specimens) bull calves, of ncb. breed, at the age of 60 to 65 days and of an average body weight 90 +/- 5 kg, was to define the effect of intramuscular acth (0.2 j.m./kg b.w.) and hydrocortisone (0.2 mg/kg b.w.) injections on the hepatic biotransformation of antipyrine. no changes were noticed in the quantity of antipyrine distribution space vd under the influence of the hormonal preparations applied. shorter time of antipyrine hal ...19911668210
diversity of pilin of serologically distinct bacteroides nodosus.pili from 11 distinct serotypes of bacteroides nodosus were examined for diversity of pilin polypeptide subunits among serotypes and for purity of the pilin preparations. the pilin of all 11 samples was shown to be homogeneous. mean +/- sd molecular weight of the pilin of 7 serotypes (a198, iv, v, vi, ix, xvii, and xviii) was 18,500 +/- 100. the pilin of serotypes i, iii, and viii had molecular weight of 17,600, 19,400, and 19,000, respectively. serotype xv differed greatly from the other 10 ser ...19911672804
electron microscopic study of bacteroides nodosus pili and associated structures.surface structures of bacteroides nodosus were examined by electron microscopy. collodion film and chrome shadowing were used for maximizing the visualization of b nodosus pili and ring structures. the existence of b nodosus pili in foot rot lesions was confirmed. contrary to previous reports, it was found that b nodosus pili production can be retained through serial broth transfer under certain conditions. capsule production by b nodosus was irregular in that it could be either lacking or varia ...19911672805
sequence of fimbrial subunit-encoding genes from virulent and benign isolates of dichelobacter (bacteroides) nodosus.both virulent and benign isolates of the ovine pathogen dichelobacter (bacteroides) nodosus produce polar fimbriae which have been implicated in twitching motility. the fimbrial subunit-encoding genes from two virulent and two benign serogroup-b isolates of d. nodosus were cloned and sequenced. analysis of the deduced amino acid (aa) sequences of these subunits indicated the presence of substitutions that appeared to correlate with the virulence phenotype. however, these aa substitutions were lo ...19911673665
organization of the fimbrial gene region of bacteroides nodosus: class i and class ii strains.the fimbrial subunit genes of bacteroides nodosus may be divided into two distinct classes, based on the sequence of the major subunit gene fima (accompanying paper--mattick et al., 1991). the genetic organization of the fibrial gene region in these two classes is also distinct. upstream of fima in both classes in opposite transcriptional orientation is the gene aroa which encodes amino acid biosynthetic enzyme 5-enolpyruvylshikimate-3-phosphate synthase. however, downstream of fima the two clas ...19911675418
gene sequences and comparison of the fimbrial subunits representative of bacteroides nodosus serotypes a to i: class i and class ii strains.we have determined the nucleotide sequences of the genes encoding the fimbrial subunits representative of the known bacteroides nodosus serogroups. all of the genes are preceded by a highly conserved region which includes the likely promoter and transcriptional regulator sites as well as the ribosome-biding site, and are followed within a short but variable distance by a sequence with the characteristics of a transcription termination or attenuation signal. based on sequence and organization, th ...19911675419
the effect of dissociation of bacteroides nodosus pili on their efficacy as a protective antigen against ovine footrot.previous studies have shown that pili from homologous bacteroides nodosus provide protective immunity in sheep against footrot, whereas denatured pilin subunits are ineffective. the aim of the present study was to examine whether pili that were dissociated into pilin subunits under less vigorous, non-denaturing treatment conditions, would provide an effective level of protective immunity. using the techniques of gel permeation chromatography, light scattering and susceptibility to proteolysis as ...19911679264
shared antigenicity and immunogenicity of type 4 pilins expressed by pseudomonas aeruginosa, moraxella bovis, neisseria gonorrhoaea, dichelobacter nodosus, and vibrio cholerae.immunoblotting with polyclonal rabbit antibodies raised against pilins expressed by pseudomonas aeruginosa, moraxella bovis, neisseria gonorrhoeae, dichelobacter nodosus, and vibrio cholerae was used to demonstrate that these polypeptides display conserved antigenic and, in most cases, immunogenic determinants. these determinants appear to be localized to the highly homologous amino-terminal domains (residues 1 to 25).19911682267
monoclonal antibodies against pili of serologically distinct bacteroides nodosus.several monoclonal antibodies (mcabs) against pili of bacteroides nodosus were examined to determine their reactivity with 11 different serotypes. one mcab was identified by enzyme-linked immunosorbent assay (elisa) analysis that bound to nine of the 11 serotypes and another that bound to the remaining two serotypes tested. in addition, some mcabs demonstrated specificity for a single serotype, while others displayed specificities for up to five other serotypes. comparison of immunoblot analysis ...19911715810
identification of three gene regions associated with virulence in dichelobacter nodosus, the causative agent of ovine footrot.dichelobacter nodosus (formerly bacteroides nodosus) is a gram-negative strict anaerobe and is the primary pathogen involved in ovine footrot. a comparative hybridization strategy was used to isolate recombinant clones which hybridized to dna from a virulent strain of d. nodosus but not with a benign isolate. three virulence-associated gene regions were identified and one of these regions was shown to be present in multiple copies in the d. nodosus genome. hybridization studies on 101 clinical i ...19911748867
the type 4 pilin of moraxella nonliquefaciens exhibits unique similarities with the pilins of neisseria gonorrhoeae and dichelobacter (bacteroides) nodosus.moraxella nonliquefaciens is a bacterium which is part of the normal flora of the human upper respiratory tract and is an occasional cause of disease. using a previously cloned type 4 pilin gene (tfpq) from moraxella bovis as a hybridization probe, we have cloned an 826 bp sau3 ai fragment which contains an m. nonliquefaciens type 4 pilin gene (tfpa) from strain nctc 7784. the pilin gene is expressed in escherichia coli. we have examined nctc 7784 and nine other m. nonliquefaciens strains by gen ...19911770363
elastolytic activity of bacteroides nodosus isolated from sheep and goats with foot rot.the elastolytic activities of 82 bacteroides nodosus strains were studied. two substrates, insoluble elastin and soluble elastin, were used for this purpose. roughly 15% of the strains which did not digest insoluble elastin were elastolytic with soluble elastin, the latter providing greater sensitivity, speed, and objectivity than its insoluble counterpart.19911774339
urinary metabolites of doxapram in premature neonates.1. urine samples from 20 premature neonates who received doxapram by i.v. infusion were analysed for drug metabolites by g.l.c-mass spectrometry. 2. in addition to doxapram, all urines contained at least one metabolite, but the known metabolite, 3-ketodoxapram, was detected in only 50% of the samples, and in some instances only in trace amounts. 3. significant inter-individual differences in the metabolic pathways of doxapram were observed. 4. a total of six metabolites of doxapram were isolated ...19911796617
genomic organization of the rat aromatic l-amino acid decarboxylase (aadc) locus: partial analysis reveals divergence from the drosophila dopa decarboxylase (ddc) gene structure.aromatic l-amino acid decarboxylase (aadc) is responsible for the conversion of l-3,4-dihydroxyphenylalanine (l-dopa) and l-5-hydroxytryptophan to dopamine and serotonin, respectively, which are important neurotransmitters. we characterized genomic clones derived from the rat aadc locus by southern blot and nucleotide sequencing analyses to explore the exonal organization of the gene. our results suggest that the rat aadc gene is relatively large, containing at least 12 exons and spanning at lea ...19911797228
genomic organization of the rat aromatic l-amino acid decarboxylase (aadc) locus: partial analysis reveals divergence from the drosophila dopa decarboxylase (ddc) gene structure.aromatic l-amino acid decarboxylase (aadc) is responsible for the conversion of l-3,4-dihydroxyphenylalanine (l-dopa) and l-5-hydroxytryptophan to dopamine and serotonin, respectively, which are important neurotransmitters. we characterized genomic clones derived from the rat aadc locus by southern blot and nucleotide sequencing analyses to explore the exonal organization of the gene. our results suggest that the rat aadc gene is relatively large, containing at least 12 exons and spanning at lea ...19911797228
[overexpression of an extracellular bacteroides nodosus protease in e. coli and isolation and characterization of its promoter].bacteroides nodosus is the essential causative agent of ovine footrot. it produces extracellular proteases which involved in pathogenesis of footrot. in this paper, we report the subcloning of bacteroides nodosus protease, its overexpression in e. coli and its n-terminal polypeptide sequence. the subclone library was constructed in e. coli using sphi digested original clone (15 kb) and plasmid ptz18r and screened using immunological assay. the expression was observed using sds-page. the subclone ...19911814046
oral administration of ssg, a beta-glucan obtained from sclerotinia sclerotiorum, affects the function of peyer's patch cells.the effect of orally administered ssg, a beta-1,3-glucan obtained from the culture filtrate of a fungus, sclerotinia sclerotiorum ifo 9395, on the function of peyer's patch (pp) cells was investigated in comparison with that on spleen cells in mice. oral administration of ssg enhanced the proliferative response of pp cells to a t-cell mitogen, concanavalin a (con a), and a b-cell mitogen, lipopolysaccharide (lps), although the response of spleen cells was not affected. peyer's patch cells taken ...19911828793
product of the pseudomonas aeruginosa gene pild is a prepilin leader peptidase.the related type iv pilins produced by pseudomonas aeruginosa, neisseria gonorrhoeae, bacteroides nodosus, and moraxella bovis are synthesized as precursors with short, six- or seven-amino acid n-terminal leader peptides. we have previously observed that p. aeruginosa mutations in pild, a gene required for pilus biogenesis, result in the accumulation of unprocessed prepilin in the membrane and a general defect in the excretion of a number of extracellular enzymes. an endopeptidase activity has b ...19911901657
effects of ozonation on mutagenic activity of polycyclic aromatic hydrocarbons. 19911932848
characterization of naturally acquired antibody responses to a recombinant fragment from the n-terminus of plasmodium falciparum glycoprotein 195.antibody responses to the glycoprotein precursor of the major merozoite surface antigens of plasmodium falciparum (gp195) were investigated in acutely infected thai adults. specific igg antibody was assayed by enzyme-linked immunosorbent assay using a recombinant fragment derived from the n-terminal region of gp195 as the capture antigen. two control groups were found to be without significant cross-reacting antibody. among occupationally exposed soldiers, 84 of 85 men developed positive antibod ...19911951865
morphogenetic expression of moraxella bovis fimbriae (pili) in pseudomonas aeruginosa.type 4 fimbriae (pili) are found in a wide variety of gram-negative bacteria and are composed of small structural subunits which share significant sequence homology among different species, especially at their amino-terminal ends. previous studies demonstrating morphogenetic expression of bacteroides nodosus fimbriae from cloned subunit genes in pseudomonas aeruginosa suggested that there is a common mechanism for type 4 fimbriae assembly and that the structural subunits are interchangeable (j. ...19901970564
identification and genetic mapping of proteins encoded in the fimbrial subunit gene region of bacteroides nodosus.the fimbriae produced by the anaerobic bacterium bacteroides nodosus are important in the pathogenesis of ovine foot rot. studies on other microorganisms have shown that the genes coding for the production and assembly of fimbriae are often clustered. by the use of maxicells, transposon mutagenesis and expression vectors, we have identified several genes which are located in the fimbrial subunit gene region. antiserum was prepared against one of the proteins (88 kda) which we were able to overpr ...19901970916
pilins from the b serogroup of bacteroides nodosus: characterization, expression, and cross-protection.sequences of pilin genes from four strains of serogroup b of the ovine pathogen bacteroides nodosus have been determined. these sequences permit comparisons of amino acid sequence between pilins from different subtypes (b1, b2, b3, b4) of the b serogroup and assessment of intraserogroup variation. pili of b. nodosus strains 234 (b1) and 183 (b2) were produced by pseudomonas aeruginosa harboring a plasmid-borne b. nodosus pilin gene, and these pili were used in sheep vaccination trials. pili from ...19901971255
antigenic stability of fimbriae of bacteroides nodosus.successful vaccination of sheep against footrot and attempts to eradicate the disease depend on there being a limit to the antigenic diversity of the causative bacterium, bacteroides nodosus. fimbrial antigenic variation was therefore investigated in vivo, both under conditions of chronic infection and under the pressure of a vaccine-induced immune response, to ascertain whether this represented an obstacle to such goals. material was available from 5 experiments and although b. nodosus appeared ...19901977378
polymerase chain reaction amplification of the constant and variable regions of the bacteroides nodosus fimbrial gene.serogrouping of bacteroides nodosus is based on antigenic differences in fimbriae of the different new zealand prototype strains. because of the time needed to isolate and grow pure cultures of b. nodosus and the difficulty in distinguishing between different serogroups because of cross-agglutination, a new dna-based diagnostic approach based on the fimbrial gene sequence of b. nodosus was developed. published nucleotide sequences of the fimbrial genes for serogroups a, g, d, and h showed conser ...19901979330
characterisation of virulent and benign strains of bacteroides nodosus.the extracellular proteases of 395 isolates of b. nodosus from ovine, bovine and caprine foot lesions were classified as either thermostable or thermolabile. stable protease was associated with one and unstable protease with four distinctive isoenzyme patterns, each pattern differentiated by the relative mobility of paired isoenzymes. pathogenicity tests on 64 isolates showed a correlation between the production of stable protease and the production of virulent ovine footrot lesions. the mean va ...19912024437
cross-protective immunity and the serological classification system for bacteroides nodosus.the relationship between the serological classification system for serogroup b and for serogroup h of bacteroides nodosus and cross-protection between subgroups within these serogroups was examined. protection against ovine footrot following vaccination was achieved against other subgroup strains provided sufficient cross-reactive antibody was induced by shared pilus antigens. within serogroup b, better cross-protection against one subgroup was obtained with a pili vaccine than a whole cell vacc ...19912025201
evaluation of a new percutaneous stainless steel greenfield filter by intravascular ultrasonography.the standard stainless steel greenfield filter was modified by extending the limbs at the apex to permit loading into a 12f carrier for percutaneous insertion. this model was tested for fixation and resolution of experimental embolism in dogs (six filters in four dogs) and sheep (eight suprarenal filters in eight sheep). serial radiographs and venacavography at 2 and 4 weeks allowed measurement of migration and suspected caval penetration, confirmed by direct examination at autopsy. caval penetr ...19912042090
the recognition and prevalence of bacteroides nodosus serotype m in australia and new zealand. 19912069548
measurement of protease thermostability, twitching motility and colony size of bacteroides extracellular protease activity of bacteroides nodosus in tas liquid culture varied directly with cell mass and buffer concentration between 20 and 50 mm hepes, mops and tes buffers, but not with tris which gave anomalous high cell counts, nor with na2co3 which showed a decline of protease activity and cell mass. the stability of hepes-buffered crude protease preparations were estimated on the basis of temperature or ca2+ activity. variation of the estimates for cellular twitching was grea ...19902194364
evidence that bacteroides nodosus belongs in subgroup gamma of the class proteobacteria, not in the genus bacteroides: partial sequence analysis of a b. nodosus 16s rrna gene.the taxonomic status of the anaerobe bacteroides nodosus has for some time been uncertain. to resolve this uncertainty, the distal portion of a 16s rrna gene from this important ovine pathogen was cloned, mapped, and sequenced. a comparison of the sequence with the sequences of 16s rrna molecules from other bacteria indicated that b. nodosus is more closely related to escherichia coli and other members of the class proteobacteria than to bacteroides fragilis or the bacteroides-flavobacterium-cyt ...19902223609
isolation and identification of anaerobic bacteria from ovine foot rot in spain.a microbiological study was made of 125 merino sheep showing clinical signs of foot rot. a total of 435 strictly anaerobic strains were isolated, belonging to the following genera: bacteroides, peptostreptococcus, tissierella, fusobacterium, megasphaera, eubacterium, acidaminococcus, clostridium, peptococcus and propionibacterium. of the 35 species obtained, the following were found in more than 10 per cent of animals sampled: bacteroides nodosus, b putredinis, b buccae, b ruminicola subspecies ...19902236925
obligately anaerobic bacterial species isolated from foot-rot lesions in goats.lesions showing clinical signs of foot-rot from 120 goats were cultured on six selective media during october 1987 to november 1988. a total of 582 strictly anaerobic microorganisms belonging to 50 different species was isolated and identified. the anaerobes most frequently isolated belonged to the following genera: bacteroides (80%), peptostreptococcus (63.6%), megasphaera (40%), fusobacterium (29.2%), clostridium (22.5%), propionibacterium (12.5%), eubacterium (11.7%) and leptotrichia (10.8%). ...19902271912
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