| the effect of mixed phosphatidylcholine liposomes on beef heart cytochrome c oxidase [proceedings]. | | 1978 | 84573 |
| distribution of myosin isoenzymes among skeletal muscle fiber types. | using an immunocytochemical approach, we have demonstrated a preferential distribution of myosin isoenzymes with respect to the pattern of fiber types in skeletal muscles of the rat. in an earlier study, we had shown that fluorescein-labeled antibody against "white" myosin from the chicken pectoralis stained all the white, intermediate and about half the red fibers of the rat diaphragm, a fast-twitch muscle (gauthier and lowey, 1977). we have now extended this study to include antibodies prepare ... | 1979 | 90047 |
| the use of avidin-biotin interaction in immunoenzymatic techniques. | biotin was covalently attached to antibodies, antigens and enzymes, and the effects of this labeling on the antigen and antibody binding capacity and on enzymatic activity were tested. based on avidin-biotin interaction, the labeled proteins were used in quantitative enzyme-immunoassay and enzyme-immunohistochemical staining procedures. two procedures were developed. in the first procedure, named the bridged avidin-biotin (brab) technique four steps were used sequentially in order to quantify or ... | 1979 | 90074 |
| polyribonucleotide inhibition of ribonucleic acid directed deoxyribonucleic acid polymerase of mouse mammary tumor (type b) virus and simian sarcoma (type c) virus. | | 1979 | 90331 |
| [animal experimental studies on the volume expanding effect of hydroxyethyl starch 40000 in the dog acute hemorrhagic shock]. | after acute hemorrhagic shock in dogs the hemodynamics of hydroxyethyl starch (mw: 40,000) are studied. the experiment shows that this plasma expander has a good efficiency for 3 to 4 hours. the indications of application of this colloidal solution are discussed. | 1979 | 90651 |
| purification of a new high activity form of glucose-6-phosphate dehydrogenase from rat liver and the effect of enzyme inactivation on its immunochemical reactivity. | a new form of cytoplasmic glucose-6-phosphate dehydrogenase (e.c.1.1.1.49) was purified from rat liver by protamine sulfate precipitation, ammonium sulfate fractionation, ion exchange chromatography with diethylaminoethyl cellulose, and affinity chromatography with cibacron blue agarose and nadp agarose. this form of the enzyme has a specific activity of over 600 units/mg of protein and gives essentially a single band by polyacrylamide gel electrophoresis. the form of the enzyme isolated by this ... | 1979 | 90677 |
| the possible role of tightly bound adenine nucleotides in oxidative and photosynthetic phosphorylation. | the tightly bound nucleotides of the beff-heart mitochondrial atpase are released during cold inactivation followed by ammonium sulfate precipitation. during incubation at 0 degrees c the sedimentation coefficient (s20w) of the atpase first declines from 12.1s to 9s. prolonged incubation or precipitation with ammonium sulfate leads to dissociation of the 9s component into subunits with s20w of 3.5s. the 9s component still bears bound nucleotides which exchange more extensively and rapidly with a ... | 1975 | 127089 |
| [susceptibility testing of anaerobes (author's transl)]. | the methodology of susceptibility testing of anaerobes has not yet been standardized and many individual test methods are in use. among the factors which may influence the outcome of susceptibility tests are test organisms, antimicrobials and methods of susceptibility testing. comparative studies have shown that the density of the inoculum largely influences the mics of sulphonamides and certain other antimicrobials. in testing susceptibility of anaerobes to doxycycline, the mics determined by a ... | 1978 | 365718 |
| immunolatex localization by scanning electron microscopy of intestinal bacteria from cockroaches. | an immunolatex reagent was developed from antiserum to fusobacterium varium, an obligate anaerobe isolated from the colon of the cockroach, periplaneta americana l. a new technique that enabled the preparation of a highly efficient immunolatex conjugate was used to localize the bacterium with scanning electron microscopy, in situ, in the mixed gut contents. | 1978 | 623460 |
| microbial synergy in experimental intra-abdominal abscess. | intra-abdominal sepsis was studied in wistar rats by using four microbial species: escherichia coli, enterococci, bacteroides fragilis, and fusobacterium varium. these organisms were implanted into the peritoneal cavity singly and in all possible dual combinations. results were evaluated by mortality rates and the incidence of intra-abdominal abscesses on autopsy following sacrifice after 7 days. mortality was restricted to recipients of e. coli, thus implicating coliforms in the acute lethality ... | 1976 | 814099 |
| [susceptibility to thiamphenicol and chloramphenicol of anaerobic bacteria (author's transl)]. | the in vitro susceptibility to thiamphenicol and chloramphenicol of 272 anaerobes, most of which were recent clinical isolated, was determined by broth dilution tests, with chloramphenicol, 133 anaerobic gram-negative non-sporing rods (48 bacteroides fragilis, 13 b. thetaiotaomicron, 14 b. oralis, 16 sphaerophorus varius etc.) had mic values of 0.03 through 16 microng/ml. very similar results (mic, 0.06-16 microng/ml) were obtained with thiamphenicol. in concentrations of 4 microng/ml or less ch ... | 1977 | 848218 |
| [in vitro susceptibility of bacteroidaceae to cefoxitin and cephalothin (author's transl)]. | the activity of the new cephamycin c derivatives cefoxitin against 102 clinical isolates of bacteroidaceae (95 bacteroides strains and 7 fusobacterium-sphaerophorus cultures) was determined by tube dilution and standardized agar diffusion tests and compared to that of cephalothin. cefoxitin was more active than cephalothin against 56 bacteroides fragilis strains, 19 bacteroides thetaiota-omicron strains and two bacteroides splanchnicus strains. the difference seemed less pronounced with five bac ... | 1977 | 856727 |
| [studies on the use of wheat rich in crude proteins in the feeding of broilers. 2. carcass yields and carcass composition]. | studies were made to investigate whether the the substitution of maize for high-protein wheat fed in combination with different protein in fattening rations to broilers would influence the carcass yields and chemical carcass composition of broilers. the described dietary regime was not found to have a significant influence on the different criteria of carcass composition (roaster carcass, flesh, abdominal fat, total proportion of utilizable parts) with the exception of the relative proportion of ... | 1975 | 1233971 |
| immunolocalization of scrapie amyloid (prp27-30) in chronic wasting disease of rocky mountain elk and hybrids of captive mule deer and white-tailed deer. | scrapie amyloid-immunoreactive plaques are present in brain tissues of captive mule deer with chronic wasting disease (cwd), a progressive neurological disorder characterized neuropathologically by widespread spongiform change of the neuropil, intracytoplasmic vacuolation in neuronal perikarya and astrocytic hypertrophy and hyperplasia. we report here the immunolocalization of scrapie amyloid (prp27-30) in plaques observed in brain tissues of rocky mountain elk (cervus elaphus nelsoni) and hybri ... | 1991 | 1681473 |
| intrageneric relationships of members of the genus fusobacterium as determined by reverse transcriptase sequencing of small-subunit rrna. | the phylogenetic interrelationships of 14 members of the genus fusobacterium were investigated by performing a comparative analysis of the 16s rrna sequences of these organisms. the sequence data revealed considerable intrageneric heterogeneity. the four species fusobacterium nucleatum (including f. nucleatum subsp. nucleatum, f. nucleatum subsp. polymorphum, "f. nucleatum subsp. fusiforme," and "f. nucleatum subsp. animalis"), fusobacterium alocis, fusobacterium periodonticum, and fusobacterium ... | 1991 | 1715737 |
| the anti-tachycardic mechanism of a direct-acting vasodilator, budralazine, in rats. | the present study was undertaken to elucidate the anti-tachycardic effect of a direct-acting vasodilator, budralazine, using an electrophysiological technique. normotensive male wistar rats were used. rats were anesthetized intraperitoneally with urethane and alpha-chloralose. intravenous administration of budralazine (0.5-5.0 mg/kg) produced a dose-dependent reduction of mean arterial pressure in anesthetized rats. at doses of 0.5 and 1.0 mg/kg, budralazine induced bradycardia accompanied with ... | 1991 | 1773498 |
| selected faecal bacteria and nutrients essential for antagonism of salmonella typhimurium in anaerobic continuous flow cultures. | as few as five of the species of bacteria commonly found in human faeces--escherichia coli, enterobacter aerogenes, enterococcus faecalis, bacteroides ovatus and fusobacterium varium--when grown together in anaerobic continuous flow cultures exerted antagonistic effects on salmonella typhimurium as great as those given by mixed bacteria from extracts of human faeces. in a single culture, the population of s. typhimurium was c. 10(8) cfu/ml but in mixed cultures with the five antagonistic bacteri ... | 1991 | 1875391 |
| biological functions of the water-insoluble fraction of mouse seminal vesicle fluid. i. suppression of the blastogenic response of lymphocytes. | the effects of the water-insoluble fraction of mouse seminal vesicle fluid (wif-svf) on lymphocytes was investigated to clarify its role in reproductive immunity. wif-svf inhibited the blastogenic response of t-cells to concanavalin-a (con-a), but it did not inhibit the blastogenic response of b-cells to lipopolysaccharide (lps). pretreatment of splenocytes with wif-svf did not suppress the blastogenic response of splenocytes to con-a when treated cells were washed prior to culture. wif-svf did ... | 1991 | 1906049 |
| microbiological study of hiv-related periodontitis. | the subgingival microbiota in 14 persons with hiv-periodontitis was examined. subgingival plaque samples were collected with paper points, transported in vmga iii, and plated on anaerobic enriched brucella blood agar and various selective media. hiv-periodontitis sites revealed actinobacillus actinomycetemcomitans, wolinella recta, peptostreptococcus micros, and bacteroides intermedius, each averaging 7% to 16% of the cultivable subgingival flora in positive patients. high levels of spirochetes ... | 1991 | 2002434 |
| the role of escherichia coli haemolysin in the pathogenic synergy of colonic bacteria in subcutaneous abscess formation in mice. | the growth of nine species of colonic bacteria--escherichia coli, enterococcus faecalis, bacteroides ovatus, fusobacterium varium, clostridium perfringens, klebsiella pneumoniae, proteus vulgaris, staphylococcus aureus and bifidobacterium adolescentis--was examined after concomitant injection to form experimental subcutaneous abscesses in mice. injection of a mixture of c. 10(5) cfu of each of the first five strains (e. coli, ent. faecalis, b. ovatis, f. varium and c. perfringens) resulted in ab ... | 1990 | 2146395 |
| recognition of biovar c of fusobacterium necrophorum (flügge) moore and holdeman as fusobacterium pseudonecrophorum sp. nov., nom. rev. (ex prévot 1940). | the cellular morphology, colonial morphology, biochemical properties, dna base compositions, and dna-dna homolgies of three biovars of fusobacterium necrophorum were examined. some differences were found among the three biovars in cellular morphology, colonial morphology, and biochemical properties. the guanine-plus-cytosine contents of dnas from biovar c strains fn521t (t = type strain), fn522, and fn520 were 30.4, 29.3, and 28.0 mol%, respectively, and the guanine-plus-cytosine contents of dna ... | 1990 | 2223600 |
| regulation of embryonic smooth muscle myosin by myosin light chain kinase and by protein kinase c. | | 1989 | 2533455 |
| acetylcholine causes nicotinic depolarization in rat dorsal motor nucleus of the vagus, in vitro. | action of acetylcholine (ach) on the electrophysiologically identified preganglionic neurons in the dorsal motor nucleus of the vagus (dmv) were investigated in slice preparations of rat medulla oblongata by using conventional intracellular recording technique. applications of ach to the dmv neurons resulted in a marked depolarization, which was not abolished in ca-free superfusing solution, indicating the direct action of ach on the cells. this depolarization was associated with an increase in ... | 1989 | 2575433 |
| pathogenicity of fusobacterium necrophorum biovar c in mice by intraperitoneal and intraportal injections. | three strains of fusobacterium necrophorum biovar c were injected into mice intraperitoneally and intraportally. all the mice survived. in one mouse out of 15 mice injected intraperitoneally, a few focal abscesses were formed in the liver. the microorganisms were recovered from the liver abscess and the tissue of liver with abscess. no changes were observed in the organs of other 14 mice and no bacteria were recovered from them. in the 15 mice injected intraportally, no liver abscesses and no ma ... | 1989 | 2689741 |
| betalactam antibiotics interfere with eukaryotic dna-replication by inhibiting dna polymerase alpha. | betalactam antibiotics (bla) are the most widely used antibacterial drugs in practical medicine. recent experiments suggested that bla, especially after "aging" in aqueous solutions, have an inhibitory effect on the growth of a variety of cultured human cells by interfering with dna synthesis (neftel et al. cell biol. toxicol. 2, 513-521, 1986). our initial observation that the replicative dna polymerase alpha might be the target of the action of betalactam compounds (hübscher et al. cell biol t ... | 1987 | 2827125 |
| stringent regulation of stably integrated chloramphenicol acetyl transferase genes by e. coli lac repressor in monkey cells. | monkey cell lines that constitutively synthesize 38.6 kd lac repressor protein and bear stably integrated chloramphenicol acetyl transferase (cat) genes linked to a lac operator-containing sv40 early promoter-enhancer were generated. when grown in medium containing isopropyl beta-d-thiogalactoside (iptg), these cells acquired a cat+ phenotype. in contrast, when grown in parallel in medium lacking iptg, the cells remained cat-. maximum induction of cat activity occurred after 4 days of iptg expos ... | 1988 | 2830990 |
| ecology of colorado tick fever. | | 1988 | 2849373 |
| antiviral chemoprophylaxis for cytomegalovirus infection. | | 1988 | 2849602 |
| potent antagonism of escherichia coli, bacteroides ovatus, fusobacterium varium, and enterococcus faecalis, alone or in combination, for enteropathogens in anaerobic continuous flow cultures. | interactions between representative strains of four predominant resident bacteria of the human colon, escherichia coli, enterococcus faecalis, bacteroides ovatus, and fusobacterium varium, and strains of seven enteropathogens, yersinia enterocolitica, shigella flexneri, salmonella typhimurium, vibrio parahaemolyticus, v. cholerae serogroup non o1, staphylococcus aureus, and clostridium perfringens, were examined in studies with an anaerobic continuous flow culture system and medium resembling th ... | 1986 | 2875188 |
| structure of bordetella pertussis peptidoglycan. | bordetella pertussis tohama phases i and iii were grown to the late-exponential phase in liquid medium containing [3h]diaminopimelic acid and treated by a hot (96 degrees c) sodium dodecyl sulfate extraction procedure. washed sodium dodecyl sulfate-insoluble residue from phases i and iii consisted of complexes containing protein (ca. 40%) and peptidoglycan (60%). subsequent treatment with proteinase k yielded purified peptidoglycan which contained n-acetylglucosamine, n-acetylmuramic acid, alani ... | 1987 | 2887547 |
| regulation of net bicarbonate transport in rabbit cortical collecting tubule by peritubular ph, carbon dioxide tension, and bicarbonate concentration. | the effects of changes in peritubular ph, carbon dioxide tension (pco2), and hco3- concentration on net hco3- transport was examined in in vitro perfused cortical collecting tubules (ccts) from unpretreated new zealand white rabbits. lowering peritubular hco3- concentration and ph by reciprocal replacement of hco3- with cl-, significantly stimulated net hco3- absorption. lowering peritubular hco3- concentration and ph, by substitution of hco3- with gluconate, while keeping cl- concentration cons ... | 1986 | 3084564 |
| [comparative study of the progestagenic action of 16 alpha, 17 alpha-cyclohexanoprogesterones (pregna-d'6-pentarans) when administered by different routes]. | a comparative study of the progestational activity of modified pentarans depending on the dose and the route of administration was carried out. it was shown that modification of the molecule of 16 alpha, 17 alpha-cyclohexanoprogesterones by introducing and additional double bond in positions 6, 7 and methyl group in position 6 provided a high gestational effect, being particularly pronounced at intramuscular administration. | 1988 | 3191979 |
| [medico-genetic studies of human provisional organs]. | | 1987 | 3318529 |
| comparative immunological studies on corynebacterium parvum polysaccharide and fusobacterium varium lipopolysaccharide. | | 1987 | 3435242 |
| arylsulfotransferase iv catalyzed sulfation of 1-naphthalenemethanol. | | 1986 | 3464166 |
| intergeneric protoplast fusion between fusobacterium varium and enterococcus faecium for enhancing dehydrodivanillin degradation. | intergeneric protoplast fusion between fusobacterium varium (pcs glu+) and enterococcus faecium (pcr glu-) was performed under strictly anaerobic conditions to improve dehydrodivanillin (ddv) degradation. the fusion frequency obtained from the selective medium (pc+ glu-) was about 0.9 x 10(-5) to 1.3 x 10(-5). the seven fusants isolated were all gram-negative anaerobes with rod shapes like that of f. varium and with main phenotypical properties of cocci like those of e. faecium such as esculin a ... | 1987 | 3579269 |
| bvd virus infection and embryo transfer. | | 1986 | 3716157 |
| action of surfactants on porcine heart malate dehydrogenase isoenzymes and a simple method for the differential assay of these isoenzymes. | the cationic surfactant, cetyl (hexadecyl) trimethylammonium bromide (ctab), completely inactivates porcine heart cytoplasmic malate dehydrogenase (l-malate:nad+ oxidoreductase, ec 1.1.1.37) at concentrations (of surfactant) which do not affect the activity of the mitochondrial isoenzyme. these concentrations are close to, or higher than, the critical micelle concentration of ctab. an increase in the ionic strength of the medium significantly retards the ctab-induced inactivation of the cytoplas ... | 1986 | 3768404 |
| protoplast formation and regeneration of dehydrodivanillin-degrading strains of fusobacterium varium and enterococcus faecium. | two strains of rumen anaerobes isolated from dehydrodivanillin-degrading cultures were identified as fusobacterium varium and enterococcus faecium. these organisms degraded dehydrodivanillin synergistically to 5-carboxymethylvanillin and vanillic acid. specific conditions for protoplast formation and cell wall regeneration for both bacteria were determined, under strictly anaerobic conditions, to be as follows. the cell wall of each bacterium in yeast extract medium was loosened by adding penici ... | 1986 | 3777921 |
| serum urate concentrations in the dalmatian coach hound. | the mean concentration of urate in the serum of 80 dalmatian coach hounds was approximately double that in the serum of 99 dogs of other breeds. both values were higher than those reported previously. | 1985 | 3837799 |
| imipenem/cilastatin in the treatment of intraabdominal infections: a review of worldwide experience. | imipenem is a new broad-spectrum beta-lactam antibiotic, which when administered with cilastatin may be a suitable alternative to the combination of an aminoglycoside and either clindamycin or a nitroimidazole as therapy for intraabdominal infections. the clinical and microbiologic results for 164 assessable patients given imipenem/cilastatin for intraabdominal infections are reported. only three of 416 bacterial strains isolated prior to therapy from intraabdominal infection sites were resistan ... | 1985 | 3901215 |
| concomitant immunohistochemical localization of fibronectin and collagen in schistosome granulomata. | affinity purified antibodies to human collagen type i and iii and antihuman fibronectin, were employed immunohistochemically on formalin-fixed, paraffin-embedded human tissue containing schistosome granulomata. the distribution of the three connective tissue proteins was studied in 45 biopsies in which there was schistosoma haematobium (30) and schistosoma mansoni (15) infestation. the granulomata were divided into early, intermediate and late according to the proportion of inflammatory cell typ ... | 1985 | 3906066 |
| canine brucellosis in a household. | three cases of canine brucellosis from the same household were discovered during a serosurvey of privately owned dogs. one dog was found to be seropositive and culture-positive, 1 dog was seropositive, and 1 dog had suspect test results, with eye lesions suggestive of brucella canis infection. all dogs had signs seen with b canis infection, but none was pathognomonic. although pathologic findings in 2 of the dogs were consistent with findings reported by other investigators, none was conclusive ... | 1985 | 3921503 |
| response of cat skin mechanothermal nociceptors to cold stimulation. | in the cat skin, the activity of 5 mechanothermal nociceptors has been studied to cold nociceptive stimuli. the frequency of discharge is very low (maximum of 2.0 hz). the repetition of very cold stimulations decreased the discharge threshold of these receptors. after -5 degrees c stimulus, the mechanothermal nociceptors were completely inhibited during 3 to 7 minutes. these mechanothermal nociceptors should have an important function in cold pain. | 1985 | 4063842 |
| purification and properties of a phenol oxidase from the blowfly calliphora erythrocephala. | | 1973 | 4123357 |
| [demonstration of lysine decarboxylase activity in the obligately anaerobic bacterium sphaerophorus varius (author's transl)]. | | 1974 | 4151801 |
| [dependance of the gas exchange on body weight and food consumption during antithyroid and contra-insular treatment in the white rat]. | | 1966 | 4168074 |
| desmosomes in human seminiferous epithelium. an electron microscopic study. | | 1974 | 4216139 |
| [anti-lymphocyte serum, bone marrow and immunological tolerance in the young dog]. | | 1967 | 4229530 |
| biomechanical and biochemical changes in the periarticular connective tissue during contracture development in the immobilized rabbit knee. | | 1974 | 4281375 |
| [isolation of foot-and-mouth disease virus in sheep]. | | 1970 | 4316723 |
| [the replicative complexes of sendai virus]. | | 1971 | 4332921 |
| proceedings: active enhancement of mouse skin allografts: the effect of bordetella pertussis and regional lymphadenectomy. | | 1974 | 4364996 |
| [the etiological structure of dysentery in the city of yerevan based on bacteriological and immunological studies]. | | 1972 | 4573289 |
| intramuscularly administered krypton clathrate kr 85. measurement of acute changes in regional blood flow. | | 1973 | 4751835 |
| effects of exogenous renin and sodium load on renin activity in unilaterally nephrectomized rats. | | 1973 | 4795631 |
| dna synthesis in cell-free extracts of a dna polymerase-defective mutant. | | 1970 | 4933688 |
| rifampin-blood-agar as a selective medium for the isolation of certain anaerobic bacteria. | a selective medium which allows detection of relatively small numbers of fusobacterium varium in fecal specimens is described. blood-agar containing 50 mug of rifampin per ml inhibits the growth of many species of bacteriodes and of f. fusi-forme/nucleatum but allows good growth of f. varium and most strains of f. mortiferum. quantitative cultures of 11 fecal specimens were done on rifampin and other selective and nonselective media. f. varium was recovered in counts of 10(6) and 10(7) per gram ... | 1971 | 4943582 |
| ultrastructural studies of sphaerophorus varius and the infective process of an associated bacteriophage. | | 1972 | 5070709 |
| tryptophanase in diverse bacterial species. | the distribution of tryptophanase was studied. the highest observed specific activity, mumoles per minute per milligram (dry weight) cells, is given in parentheses after each species. tryptophanase was inducible and repressible in escherichia coli (.914), paracolobactrum coliforme (.210), proteus vulgaris (.146), aeromonas liquefaciens (.030), photobacterium harveyi (.035), sphaerophorus varius (.021), bacteroides sp. (.048), and corynebacterium acnes (.042). the enzyme was constitutive and nonr ... | 1969 | 5781572 |
| antimicrobial drugs used in the management of anaerobic infections in children. | optimum antimicrobial therapy effective against anaerobes is required to rapidly resolve infections due to these organisms and to prevent serious complications. selection of antimicrobial therapy should be based on clinical experience and presumptive evidence until culture and sensitivity tests are available. if an abscess should develop, surgical drainage (when possible) is of paramount importance. antimicrobial therapy for anaerobic infections should usually be given for prolonged periods beca ... | 1983 | 6360636 |
| cellular fatty acid and soluble protein profiles of oral fusobacteria. | we compared the cellular fatty acid and protein content of 43 strains of oral fusobacteria isolated from patients with chronic gingivitis, acute necrotizing ulcerative gingivitis, and juvenile and adult periodontitis, as well as from the stump-tailed macaque, to that of eight reference strains of oral and non-oral fusobacterium species. a gas-liquid chromatographic examination of trimethylsilyl derivatives of the fatty acid methyl esters revealed n14:0, 3-oh-14:0, n16:0, 16:1, 3-oh-16:0, n18:0, ... | 1983 | 6581193 |
| purification and characterization of c-s lyase from fusobacterium varium. a c-s cleavage enzyme of cysteine conjugates and some s-containing amino acids. | an enzyme responsible for the carbon-sulfur bond cleavage of various s-aryl, s-aralkyl, and s-alkyl cysteines has been purified about 270-fold from fusobacterium varium. incubation of a cysteine conjugate of p-bromobenzene with the enzyme yielded equimolar amounts of p-bromobenzenethiol, pyruvic acid, and ammonia, indicating that the carbon-sulfur bond cleavage proceeds via an alpha, beta-elimination reaction. the enzyme activity was inhibited either by hydroxylamine or kcn and stabilized by pyr ... | 1984 | 6698982 |
| [in vitro sensitivity of strict anaerobic gram-negative bacilli to thienamycin nf and various cephalosporins]. | minimal inhibitory concentrations of 105 anaerobic gram negative bacilli were determined by two-fold dilution in wilkins - chalgren agar following the reference method proposed by sutter et al. no resistance was observed with thienamycin, which is especially active against the bacteroides fragilis group (mic 50 = 0.125 mg/l). among the beta-lactams investigated, cefoxitin and moxalactam proved more active than ceftizoxime and ceftriaxone, while cefotiam exhibited poor activity. while most fusoba ... | 1984 | 6739150 |
| alteration in sensitivity of transmural electrical response to propionate in rat colon after chronic luminal infusion of short-chain fatty acids. | the colon is always exposed to abundant short-chain fatty acids (scfa) produced by gut fermentation. in order to know an effect of chronic load of scfa on colonic functions, we studied that the acute and chronic effects of scfa on transmural potential difference (p.d.) across the proximal colon of germ-free (gf), gnotobiotic (gb) and conventionalized (cv) rats in vivo. intravenous administration of scfa (acute effect), such as propionate, butyrate, valerate or caproate, caused a transient increa ... | 1983 | 6827889 |
| septic arthritis due to fusobacterium varium in a patient with sickle-cell anemia. | | 1983 | 6842476 |
| gram-negative anaerobic bacilli: their role in infection and patterns of susceptibility to antimicrobial agents. ii. little-known fusobacterium species and miscellaneous genera. | twenty infrequently reported species of gram-negative anaerobic bacilli other than fusobacterium nucleatum, fusobacterium necrophorum, and members of the genus bacteroides were studied with regard to their role in infection and their susceptibility to antimicrobial agents. in addition, the literature regarding the recovery of these organisms from both the normal flora and infections of humans was reviewed. during a six-year period at the wadsworth clinical anaerobic bacteriology research laborat ... | 1981 | 7025153 |
| anaerobic mastoiditis: a report of two cases with complications. | aerobic bacteria are responsible for most cases of suppurative otitis media/mastoiditis (om/m). as many as 20-30% of middle ear aspirates in acute otitis media, however, are reported to be sterile on aerobic culture. some of these cases may be due to anaerobic bacteria. two cases of om/m due to anaerobic bacteria are reported. the first patient had no antecedent ear disease and developed a large bezold abscess caused by fusobacterium varium. the second patient had attic retraction pouches bilate ... | 1982 | 7144403 |
| inactivation of anaerobic bacteria by various photosensitized porphyrins or by hemin. | the photodynamic effects of deuteroporphyrin (dp), hematoporphyrin derivative (hpd), hematoporphyrin (hp), or protoporphyrin (pp) on a variety of anaerobic microorganisms were examined in this study. the majority of the species, among the 350 strains tested, were inhibited by concentrations of < or = 2.5 micrograms/ml of light-activated dp. species found to be resistant to this treatment included bilophila wadsworthia, fusobacterium mortiferum, fusobacterium varium, and bacteroides gracilis. the ... | 1994 | 7765091 |
| activity of wy-49605 compared with those of amoxicillin, amoxicillin-clavulanate, imipenem, ciprofloxacin, cefaclor, cefpodoxime, cefuroxime, clindamycin, and metronidazole against 384 anaerobic bacteria. | the national committee for clinical laboratory standards agar dilution method was used to compare the in vitro activity of wy-49605 (also called sun/sy 5555 and alp-201), a new broad-spectrum oral penem, to those of amoxicillin, amoxicillin-clavulanate, imipenem, ciprofloxacin, cefaclor, cefpodoxime, cefuroxime, clindamycin, and metronidazole against 384 clinically isolated anaerobes. these anaerobic organisms included 90 strains from the bacteroides fragilis group, 87 prevotella and porphyromon ... | 1994 | 7872754 |
| fusobacterial infections in children. | a total of 243 strains of fusobacteria species was recovered from 226 of 1399 (16%) specimens obtained from 213 children. the strains included 65 (27%) fusobacterium sp., 144 (59%) fusobacterium nucleatum, 25 (10%) fusobacterium necrophorum, five (2%) fusobacterium varium, three (1%) fusobacterium mortiferum, and one (0.4%) fusobacterium gonidiaformans. most fusobacteria species were recovered from patients with abscesses (100), aspiration pneumonia (24), paronychia (15), bites (14), chronic sin ... | 1994 | 8034995 |
| effect of co2 on susceptibilities of anaerobes to erythromycin, azithromycin, clarithromycin, and roxithromycin. | the oxyrase agar dilution method (oxyrase, inc., mansfield, ohio), which provides an anaerobic environment without added co2, was compared with the reference agar dilution method recommended by the national committee for clinical laboratory standards (anaerobic chamber with 10% co2) to test the susceptibilities of 302 gram-negative and gram-positive anaerobes to erythromycin, azithromycin, clarithromycin, and roxithromycin. for erythromycin, the overall mic for 50% of isolates tested (mic50) was ... | 1994 | 8192445 |
| patterns of susceptibility to fluoroquinolones among anaerobic bacterial isolates in the united states. | the activity of the fluoroquinolone antibiotics against anaerobic bacteria has generally been moderate to poor. nalidixic acid and cinoxacin show poor activity against almost all anaerobes. norfloxacin and enoxacin exhibit poor anaerobic activity (mic90, 8- > 256 micrograms/ml) except against clostridium perfringens, bacteroides ureolyticus, and some eubacteria. norfloxacin is slightly more active than enoxacin against some bacteroides species. while ciprofloxacin is more active than earlier flu ... | 1993 | 8324151 |
| in vitro activity of fk037, a new parenteral cephalosporin, against anaerobic bacteria. | the activity of fk037, a new parenteral cephalosporin, was compared with those of cefpirome, ceftazidime, and flomoxef against 322 recent clinical isolates of anaerobic bacteria. a fastidious facultative anaerobe, gardnerella vaginalis, was also studied. fk037 inhibited 90% of isolates of peptostreptococcus anaerobius, peptostreptococcus asaccharolyticus, clostridium perfringens, mobiluncus spp., g. vaginalis, and porphyromonas gingivalis at < or = 0.78 micrograms/ml. the mics of fk037 for 50 an ... | 1993 | 8517721 |
| a simulation of microbial competition in the human colonic ecosystem. | many investigations of the interactions of microbial competitors in the gastrointestinal tract used continuous-flow anaerobic cultures. the simulation reported here was a deterministic 11-compartment model coded by using the c programming language and based on parameters from published in vitro studies and assumptions were data were unavailable. the resource compartments were glucose, lactose and sucrose, starch, sorbose, and serine. six microbial competitors included indigenous nonpathogenic co ... | 1996 | 8837418 |
| antianaerobic activity of the ketolide ru 64004 compared to activities of four macrolides, five beta-lactams, clindamycin, and metronidazole. | agar dilution methodology (with added oxyrase in the case of the macrolide group to allow incubation without added co2) was used to compare the activity of ru 64004, a new ketolide, with the activities of erythromycin, azithromycin, clarithromycin, roxithromycin, clindamycin, amoxicillin with and without clavulanate, piperacillin with and without tazobactam, metronidazole, and imipenem against 379 anaerobes. overall, ru 64004 yielded an mic at which 50% of the isolates are inhibited (mic50) of 1 ... | 1997 | 9145865 |
| comparative antianaerobic activities of the ketolides hmr 3647 (ru 66647) and hmr 3004 (ru 64004). | hmr 3647 (ru 66647) and hmr 3004 (ru 64004), two ketolides, had mics at which 50% of the strains are inhibited (mic50s) of 0.06 to 0.125 microg/ml and mic90s of 16.0 microg/ml against 352 anaerobes. mic50s and mic90s of erythromycin, azithromycin, clarithromycin, and roxithromycin were 0.5 to 2.0 microg/ml and 32.0 to >64.0 microg/ml, respectively. hmr 3647 and hmr 3004 were more active against non-bacteroides fragilis-group anaerobes (other than fusobacterium mortiferum, fusobacterium varium, a ... | 1997 | 9303406 |
| characterization of a novel bacteriophage in fusobacterium varium. | | 1997 | 9310709 |
| subgingival microbiota of shallow periodontal pockets in individuals after head and neck irradiation. | this study aimed at investigating the subgingival plaque microorganisms of shallow pockets (< or = 5 mm) in subjects who previously received irradiation in the head and neck region for treatment of nasopharyngeal carcinoma. direct microscopy and anaerobic culture were used. subgingival paper point samples were taken from 6 tooth-sites (one/sextant) per subject for direct microscopy (n = 108). another set of paper points was taken from the deepest of the previously selected sites (one per subject ... | 1998 | 9573815 |
| subgingival microflora associated with nifedipine-induced gingival overgrowth. | the purpose of this study was to examine the composition of subgingival plaque of 140 periodontal lesions in 35 patients with cardiovascular disorders who were administered nifedipine and manifested nifedipine-induced gingival overgrowth (go). age was inversely associated with the go. plaque index and bleeding index showed a significant association with go, while nifedipine dosage and duration of nifedipine therapy were not found to be significant predictors of go. the gingival inflammation as e ... | 1998 | 9660335 |
| in vitro activity of gemifloxacin (sb 265805) against anaerobes. | gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ... | 1999 | 10471570 |
| utilization of d-amino acids by fusobacterium nucleatum and fusobacterium varium. | the utilization of d- and l-amino acids with acidic, basic or polar side chains was demonstrated by hplc. two species of the anaerobe fusobacterium utilized d-lysine and the l isomers of glutamate, glutamine, histidine, lysine and serine. only f. varium used l-arginine, d-glutamate and d-serine as substrates, whereas f. nucleatum specifically utilized d-histidine and d-glutamine. d-glutamate accumulated in f. nucleatum cultures supplemented with d-glutamine, and ornithine was detected when eithe ... | 1999 | 10524276 |
| activities of gemifloxacin (sb 265805, lb20304) compared to those of other oral antimicrobial agents against unusual anaerobes. | the activities of gemifloxacin (sb 265805, lb20304) and comparator agents were determined by an agar dilution method against 419 clinical strains of less-commonly identified species of anaerobes. gemifloxacin was generally more active than trovafloxacin against gram-positive strains by one to two dilutions. peptostreptococci (peptostreptococcus asaccharolyticus, peptostreptococcus magnus, peptostreptococcus micros, and peptostreptococcus prevotii) and porphyromonas spp. (porphyromonas asaccharol ... | 1999 | 10543754 |
| synergic activity, for anaerobes, of trovafloxacin with clindamycin or metronidazole: chequerboard and time-kill methods. | chequerboard titrations were used to test the activity of trovafloxacin, alone and in combination with clindamycin or metronidazole, against 156 gram-positive or gram-negative anaerobes, including 47 bacteroides fragilis group, 36 prevotella spp., 26 fusobacteria, 21 peptostreptococci and 26 clostridia. mic50/mic90 values (mg/l) of each drug alone against all 156 strains were: trovafloxacin, 0.5/1; clindamycin, 0.25/2; metronidazole, 1/2. synergy (fic indices </= 0.5) was seen in two strains wit ... | 2000 | 10797085 |
| the human gut bacteria bacteroides thetaiotaomicron and fusobacterium varium produce putrescine and spermidine in cecum of pectin-fed gnotobiotic rats. | pectin is a soluble indigestible polysaccharide that stimulates cecal polyamine formation in rats. bacteroides and fusobacteria, two numerically dominant bacterial population groups in the large intestine, were found to synthesize in vitro high amounts of spermidine and putrescine. the purpose of this study was to elucidate the effect of pectin on the polyamine production by defined bacterial species in vivo. germfree male wistar rats (n = 18) were randomly assigned to one of three treatments: ( ... | 2000 | 10801923 |
| comparative in vitro activities of abt-773 against 362 clinical isolates of anaerobic bacteria. | the activity of abt-773, a novel ketolide antibiotic, against clinical isolates of anaerobic bacteria was determined and compared to the activities of other antimicrobial agents. mics at which 90% of isolates were inhibited (mic(90)s) were </=0.06 microg/ml for actinomyces spp., clostridium perfringens, peptostreptococcus spp., propionibacterium spp., and porphyromonas spp. the mic(50)s and mic(90)s were </=0.06 and >32 microg/ml, respectively, for eubacterium spp., lactobacillus spp., clostridi ... | 2001 | 11120995 |
| purification and characterization of beta-methylaspartase from fusobacterium varium. | beta-methylaspartase (ec 4.3.1.2) was purified 20-fold in 35% yield from fusobacterium varium, an obligate anaerobe. the purification steps included heat treatment, fractional precipitation with ammonium sulfate and ethanol, gel filtration, and ion exchange chromatography on deae-sepharose. the enzyme is dimeric, consisting of two identical 46 kda subunits, and requires mg2+ (km = 0.27+/-0.01 mm) and k+ (km = 3.3+/-0.8 mm) for maximum activity. beta-methylaspartase-catalyzed addition of ammonia ... | 2001 | 11506174 |
| 5s ribosomal ribonucleic acid sequences in bacteroides and fusobacterium: evolutionary relationships within these genera and among eubacteria in general. | the 5s ribosomal ribonucleic acid (rrna) sequences were determined for bacteroides fragilis, bacteroides thetaiotaomicron, bacteroides capillosus, bacteroides veroralis, porphyromonas gingivalis, anaerorhabdus furcosus, fusobacterium nucleatum, fusobacterium mortiferum, and fusobacterium varium. a dendrogram constructed by a clustering algorithm from these sequences, which were aligned with all other hitherto known eubacterial 5s rrna sequences, showed differences as well as similarities with ... | 1989 | 11542181 |
| association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid. | the objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (ast) in gingival crevicular fluid (gcf) using the colorimetric periogard (ptm) test and the subgingival microflora in early onset periodontitis lesions. | 2001 | 11737506 |
| determination of diamino acids in peptidoglycans from anaerobic bacteria. | peptidoglycans isolated from two fusobacterium species of anaerobic bacteria were analyzed for constituent amino acids. hydrolysis conditions were varied to optimize the yield of diamino acids from peptidoglycan. the o-phthalaldehyde derivatives of the diamino acid stereoisomers were separated by high-performance liquid chromatography (opa-hplc), and variations in the relative areas of the two peaks noticed during analysis of solid samples were attributed to sampling errors. co-derivatization/in ... | 2001 | 11764403 |
| [retrospective analysis of fusobacterium associated infections; experience at aomori prefectural hospital with 108 cases from 1995 to 1999]. | we experienced 108 cases of fusobacterium associated infections, including otolaryngeal, oral, pleuropulmonary, intraabdominal, skin and soft tissue infections, at aomori prefectural hospital during the 5 year-period from 1995 to 1999. a total of 433 organisms, included 113 fusobacterium spp. (80 fusobacterium nucleatum, 18 fusobaterium necrophorum, 5 fusobacterium varium, 4 fusobacterium mortiferum, 6 fusobacterium spp.), were recovered with an average of 4.0 organisms per case of the 108 cases ... | 2002 | 11852471 |
| antimicrobial susceptibility of non-bacteroides fragilis group anaerobic gram-negative bacilli in europe. | objective: to investigate the in vitro activity of a range of anti-anaerobe antimicrobials against non-bacteroides fragilis group anaerobic gram-negative bacilli isolated in europe. methods: isolates from 15 laboratories in 13 countries were identified by conventional methods. the mics of 20 antibiotics were determined by an agar dilution method. results: there were 488 prevotella spp., 174 fusobacteria, 69 porphyromonas spp., 33 bacteroides spp., 28 bilophila wadsworthia and 16 campylobacter sp ... | 1999 | 11853565 |
| 16s-23s rdna internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus fusobacterium. | the 16s-23s rdna internal transcribed spacer (its) regions of all currently defined fusobacterium species and related taxa such as leptotrichia buccalis, sebaldella termitidis and streptobacillus moniliformans, were analysed to examine inter- and intraspecies as well as subspecies relationships. for the its-amplification, a new eubacterial universal primer pair was designed and used. the majority of the fusobacterium strains, along with l. buccalis showed one major, and two to three weaker, dist ... | 2002 | 11931161 |
| comparison of the 16s-23s rrna intergenic spacer regions between fusobacterium varium and "fusobacterium pseudonecrophorum" strains. | the 16s-23s rrna intergenic spacer regions (isrs) of five strains of "fusobacterium pseudonecrophorum" which had been proposed as a new species, were compared with those of f. varium atcc 8501t. all the strains of "f. pseudonecrophorum" exhibited of sequence similarities of 97.7% to 100% to the strain of f. varium in their 16s-23s rrna isr sequences. this indicates that the strains of "f. pseudonecrophorum" and the type strain of f. varium are identical at the species level. | 2002 | 11999453 |
| fusobacterium varium localized in the colonic mucosa of patients with ulcerative colitis stimulates species-specific antibody. | microbial agents are a possible cause of ulcerative colitis. we have previously reported evidence of bacteria invading the colonic mucosa of patients with ulcerative colitis. we have isolated bacteria from inflamed colonic mucosa, examined the localization of the species in the mucosa, and assayed for serum antibodies to the bacteria. | 2002 | 12164960 |
| update on the taxonomy and clinical aspects of the genus fusobacterium. | the genus fusobacterium currently includes 13 species. fusobacterium nucleatum, the most frequently encountered species in humans, is heterogeneous and currently includes 5 subspecies. a potentially new subspecies of f. nucleatum that is intrinsically quinolone-resistant and phylogenetically separate from the other 5 subspecies has been identified from dog and cat oral flora. two subspecies have been described for fusobacterium necrophorum, and a new species, fusobacterium equinum, which is rela ... | 2002 | 12173104 |
| induction of experimental ulcerative colitis by fusobacterium varium isolated from colonic mucosa of patients with ulcerative colitis. | bacteria are implicated in certain forms of model chronic colitis but the identity and role of bacteria in human ulcerative colitis (uc) are uncertain. | 2003 | 12477765 |
| in vitro activities of ramoplanin, teicoplanin, vancomycin, linezolid, bacitracin, and four other antimicrobials against intestinal anaerobic bacteria. | by using an agar dilution method, the in vitro activities of ramoplanin, teicoplanin, vancomycin, linezolid, and five other agents were determined against 300 gram-positive and 54 gram-negative strains of intestinal anaerobes. ramoplanin was active at <or=2 microg/ml against 287 of 300 (95.7%) gram-positive organisms, including 18 strains of clostridium difficile for which mics of ramoplanin were 0.25 to 0.5 microg/ml; for 3 of these, linezolid mics were 8 to 16 micro g/ml. nineteen clostridium ... | 2003 | 12821492 |
| [study of a bacteriophage active on an anaerobic bacterium: sphaerophorus varius]. | | 1964 | 14156598 |
| phylogenetic analysis of fusobacterium necrophorum, fusobacterium varium and fusobacterium nucleatum based on gyrb gene sequences. | the nucleotide sequences of the dna gyrase b subunit gene (gyrb) of fusobacterium necrophorum subsp. necrophorum, f. necrophorum subsp. funduliforme and f. varium were determined and analyzed together with those of f. nucleatum subsp. nucleatum and f. nucleatum subsp. vincentii. on the phylogenetic tree constructed, the strains of each fusobacterial species formed distinct clusters with deep sublines. the degree of sequence similarity within each cluster was 93.2% or more, whereas similarities b ... | 2004 | 15528856 |