| [human genetics: a task for the public health service? (author's transl)]. | | 1977 | 143637 |
| lysergic acid diethylamide: evidence for stimulation of pituitary dopamine receptors. | lysergic acid diethylamide (lsd), 0.05 mg/kg and 0.20 mg/kg, significantly decreased plasma prolactin (prl) levels in male rats. lsd, 0.20 mg/kg, also inhibits the increase in plasma prl levels produced by chlorpromazine (cpz), 5 mg/kg, and alpha-methyl-paratyrosine (ampt), 50 mg/kg, both of which interfere with dopaminergic inhibition of prl secretion. lsd was more potent than methysergide, a serotonin receptor blocker, in lowering plasma prl levels and more potent than apomorphine, a known dir ... | 1977 | 143671 |
| reversible hypothyroidism in growth hormone-deficient children treated with human growth hormone. | six children with human growth hormone (hgh) deficiency became hypothyroid during the course of their therapy with hgh. this was accompanied by a decreasing growth rate, clinical symptoms of hypothyroidism and decreased serum t4 concentrations. three of the 6 patients returned to the euthyroid state, both clinically and biochemically, with cessation of hgh therapy, and reinstitution of hgh precipitated hypothyroidism again in 2 of the three. the patients who remained hypothyroid have evidence of ... | 1975 | 165211 |
| incidence and pathological features of spontaneous tumors in athymic nude mice. | systematic observation of 1141 nude mice (swiss background [corrected] strain) that received human tumor transplants revealed 24 spontaneous tumors, 18 of lymphoreticular origin and 6 pulmonary adenomas. spontaneous tumors were seen at an average age of 9.1 months, and 22 of the tumors were seen only in that fraction of our group (324 mice) surviving for 5 months or more (22 of 324 x 100 = 6.8%). transplantation of these tumors to other nude mice was successful in three of five cases. mice trans ... | 1979 | 427772 |
| interactions of folch-lees proteolipid apoprotein with planar lipid bilayers. | water-soluble folch-lees proteolipid apoprotein from bovine cns white matter induces a voltage-dependent conductance in black lipid membranes. na+ is required for the induced conductance change but the established conductance has very low ionic selectivity. the induced conductance fluctuates with a minimum amplitude of 10(-11)--10(-10) mho. the magnitude of the conductivity change is dependent on protein concentration and on the composition of lipid bilayers. at a fixed voltage the induced condu ... | 1979 | 522128 |
| urease assay and urease-producing species of anaerobes in the bovine rumen and human feces. | a growth medium and test were developed for rapid detection of urease in fermentative anaerobic bacteria. using nonselective rumen fluid roll-tube agar medium and the new test, it was confirmed that peptostreptococcus productus is often the most numerous urease-forming species in human feces. also, some fecal strains of ruminococcus albus, clostridium innocuum, and clostridium beijerinckii produced urease. single strains of fusobacterium prausnitzii, coprococcus catus, and streptococcus mitis th ... | 1977 | 879770 |
| transfer of toxin susceptibility to plant protoplasts via the hemlmintosporoside binding protein of sugarcane. | | 1975 | 1169066 |
| 2,6-dichlorophenolindophenol is a competitive inhibitor for xanthine oxidase and is therefore not usable as an electron acceptor in the fluorometric assay. | xanthine oxidase has been recognized as an important source of oxygen free radicals in ischemia-reperfusion injury. in order to study this enzyme in biological tissues, the conversion of pterin (2-amino-4-hydroxypteridine) to isoxanthopterin provides the basis for a very sensitive fluorometric assay. xanthine oxidase is typically assayed in the presence of pterin only, while an electron acceptor which replaces nad+ is used to determine the combined xanthine dehydrogenase plus xanthine oxidase ac ... | 1992 | 1563644 |
| differentiation of growth signal requirement of b lymphocyte precursor is directed by expression of immunoglobulin. | during b cell differentiation, at least three stages can be defined in terms of their growth signal requirement by using two different growth signals, which are recombinant interleukin 7 (il-7) and a stromal cell clone pa6 which does not produce il-7; first a pa6 dependent stage, second a pa6 + il-7 dependent stage and third an il-7 dependent stage. in order to test the possibility that this differentiation of growth signal requirement is controlled by the expression of functional immunoglobulin ... | 1991 | 1899373 |
| [new antitubercular drugs]. | | 1990 | 2168041 |
| sporidesmin and gliotoxin induce cell detachment and perturb microfilament structure in cultured liver cells. | changes in cell morphology and cell adhesion occurred when cultured cells from the rat liver cell strain c3 were exposed to the fungal toxins, sporidesmin or gliotoxin. both toxins caused loss of attachment of the cells to the plastic of tissue culture plates and this effect was preceded by loss of actin cables. other changes included cytoplasmic vacuolation and blocked entry into s-phase of the cell cycle. under these conditions [3h]thymidine incorporation into the cells was also diminished but ... | 1986 | 2432076 |
| acetylcholine causes nicotinic depolarization in rat dorsal motor nucleus of the vagus, in vitro. | action of acetylcholine (ach) on the electrophysiologically identified preganglionic neurons in the dorsal motor nucleus of the vagus (dmv) were investigated in slice preparations of rat medulla oblongata by using conventional intracellular recording technique. applications of ach to the dmv neurons resulted in a marked depolarization, which was not abolished in ca-free superfusing solution, indicating the direct action of ach on the cells. this depolarization was associated with an increase in ... | 1989 | 2575433 |
| assessing clamp-related vascular injuries by measurement of associated vascular dysfunction. | the development of vascular clamps requires a reliable method to quantitate clamp-related vascular injuries. the degree of vessel damage usually is estimated subjectively from photomicrographs made with scanning electron microscopy. in order to test whether the use of vascular rings to assess residual vascular function may be a better method, rabbit thoracic aortas were occluded by five types of clamps: a fogarty softjaw bulldog, a fogarty hydragrip, a 6-inch satinsky clamp, an edslab bulldog, a ... | 1989 | 2648627 |
| dna alkylation and formation of dna interstrand cross-links by potential antitumour 2,5-bis(1-aziridinyl)-1,4-benzoquinones. | a series of 3,6-substituted 2,5-bis(1-aziridinyl)-1,4-benzoquinone derivatives was shown to alkylate calf thymus dna and to form dna interstrand cross-links. alkylation and cross-link formation were enhanced after electrochemical reduction of the compounds and increased with lower ph in the ph range from 4.5 to 8.0. reduction especially shifts the ph at which cross-linking and alkylation occurs to higher values, which are more physiologically relevant. this shift is probably caused by the increa ... | 1989 | 2663197 |
| [the hormonal response in bulls to the administration of zeranol and rumensin]. | the objective of the study was to investigate the influence of the growth stimulators ralgro zeranol and rumensin on changes in concentrations of certain hormones in the blood of beef bulls. in the first experiment 21 bulls were investigated which were divided into three groups. the first group was control, the second was administered the ralgro stimulator (3x) and the third the ralgro and rumensin stimulators. in the second experiment there were 12 bulls. the animals in the test group were impl ... | 1989 | 2667252 |
| minitek urea disk test, a sensitive and cost-effective method to screen for campylobacter pylori in gastric biopsies. | one hundred fifty-five biopsy specimens from the gastric mucosa of 81 patients undergoing routine endoscopy procedures were tested for the presence of campylobacter pylori by three methods: gram stain, culture, and modified minitek, a rapid urea disk test (bbl microbiology systems, cockeysville, md.). twenty-nine patients were infected with c. pylori. sensitivities and specificities of detection were 100 and 94% with the minitek test and 93 and 100% with gram stain, respectively. rapid testing b ... | 1989 | 2687317 |
| falciparum malaria--present day problems. an experience with 425 cases. | clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ... | 1989 | 2693436 |
| use of the glucose oxidase/peroxidase method for glucose assay leads to overestimation of the inhibition of gluconeogenesis by aminopyrine. | 4-aminoantipyrine strongly inhibits glucose determination by the glucose oxidase/peroxidase/dianisidine assay but does not interfere in the assay using glucose-6-phosphate dehydrogenase, hexokinase and atp. as a result, the inhibition of gluconeogenesis by aminopyrine reported to be 50-90% (bánhegyi, g., mandl, j., antoni, f. and garzó, t. (1987) biochim. biophys. acta 927, 406-416) is strongly overestimated and amounts to only 10-30%. | 1989 | 2713417 |
| protective role of serum antibody in immunity to chlamydial genital infection. | female guinea pigs were injected intraperitoneally with pooled immunoglobulin derived from animals immunized to the chlamydial agent of guinea pig inclusion conjunctivitis. genital infections in animals receiving pooled immunoglobulin from immune animals were markedly decreased with regard to the number of inclusions detected compared with control animals. these data indicated that serum-derived antibody was able to provide a degree of protection against a chlamydial genital tract infection. | 1989 | 2909492 |
| ischaemic mouse thigh model for evaluation of pathogenicity of non-clostridial anaerobes. | a mouse thigh model has been devised in which the growing culture of non-clostridial anaerobe in the ischaemic tissue produces inflammatory swelling and death. the swelling of the right thigh served as an index of pathogenicity of the test strain in comparison to the negatively reacting left thigh which received injection of the control strain of bifidobacterium infantis. actinomyces naeslundii exceptionally caused death in all animals within 24 h. mortality and thigh swelling were pronounced (g ... | 1989 | 2914729 |
| [septicemic campylobacteriosis after dog bite]. | | 1986 | 2945190 |
| effect of mononuclear cell factors on calcium transport in sarcoplasmic reticulum vesicles. | the effect of supernatants from cultures of mitogen-stimulated human mononuclear cells on calcium transport by sarcoplasmic reticulum was examined. calcium transport was assayed by measuring the time course of calcium accumulation by sarcoplasmic reticulum incubated with supernatants from stimulated mononuclear cells was 20% less than that by vesicles exposed to control supernatants (p less than 0.001). in contrast, no difference in calcium-dependent atpase activity was noted between vesicles in ... | 1986 | 2946307 |
| demonstration of non-degraded aleutian disease virus (adv) proteins in lung tissue from experimentally infected mink kits. brief report. | isolates of adv replicate to rather high quantities in lungs from neonatally infected mink kits. the virus was analysed for polypeptide composition, and for the first time high molecular weight polypeptides have been observed in in vivo produced advs. these polypeptides are analogous to those of in vitro produced advs. the molecular weights of the structural polypeptides of the low virulence pullman adv and the highly virulent dk and utah i isolates of adv were found to be 88kd and 78kd and in v ... | 1986 | 3002299 |
| central nervous system lesions in suckling mice and rats inoculated intranasally with sialodacryoadenitis virus. | suckling cd-1 and cfw mice and wi wistar rats were inoculated intranasally with sialodacryoadenitis virus. in animals inoculated during the first week of life, there was an acute necrotizing encephalitis with malacia and minimal inflammatory cell response. in mice and rats which survived for up to 12 days post inoculation, loss of brain substance, gitter cells and occasionally mineralized debris were seen. viral antigen was readily demonstrated in neurons and nasal epithelium in trypsin-treated, ... | 1986 | 3004003 |
| epstein-barr virus infections in families: the role of children with infectious mononucleosis. | we performed a prospective evaluation of epstein-barr virus (ebv) infections in 78 families with a childhood index case of ebv-infectious mononucleosis (ebv-im). during the acute index episode, adult family (household) contacts, compared with control adults, had a greater rate of oropharyngeal ebv excretion and high serum antibody responses, which suggested a recent antecedent reactivation of an old ebv infection. the increased prevalence of oropharyngeal ebv and acute serological responses foun ... | 1986 | 3021867 |
| release of low infectivity vesicular stomatitis virus particles from tunicamycin-treated cells. | the effect of tunicamycin (tm) treatment on the production, glycosylation, morphology and infectivity of vesicular stomatitis virus (vsv) released from mouse (lb), monkey (cos-1 and vero), bovine (mdbk), hamster (bhk) and human (hela, u and gm2504) cells was investigated. the yield of vsv particles released from tm treated cells, as measured by total viral proteins, was inhibited only 2-10 fold while the infectivity of these particles was greatly reduced (10-1000 fold). the morphology of vsv par ... | 1985 | 3023584 |
| initiation of swimming activity by trigger neurons in the leech subesophageal ganglion. ii. role of segmental swim-initiating interneurons. | cell tr1, a trigger neuron found in the subesophageal ganglion of the leech, hirudo medicinalis, is part of a network of subesophageal ganglion neurons which control swimming activity, and makes apparently direct connections to swim-initiating interneurons (siis; cells 204 and 205). in this study, we investigated the role of siis in swim initiation by cell tr1. we also examined how brief tr1 activity controls swim initiation at the levels of the siis and of the oscillator neurons. we found: in s ... | 1986 | 3023603 |
| immunosuppressive properties of human placenta: study of supernatants from short-term syncytiotrophoblast cultures. | using collagenase and mechanical treatment to attempt to eliminate cellular contamination such as macrophages and decidual cells, trophoblast enriched cell suspensions were isolated from the human placenta. with a view to assessing the role of trophoblast in impairing maternal rejection of the fetus, supernatants (spl4) were prepared from these placental cells after short-term culture (4 h). the immunosuppressive activity of these supernatants was studied following application to mitogen-stimula ... | 1986 | 3023607 |
| synthesis and antiviral evaluation of 1,4-dioxane nucleoside analogues related to nucleoside dialdehydes. | since biologically active nucleoside 2',3'-dialdehydes exist as six-membered cyclic acetals (1) in solution, we have investigated the antiviral activity of some structurally similar 1,4-dioxane nucleoside analogues. by reacting 2',3'-seconucleoside tosylates with base, the guanine (10) and adenine (18) substituted (hydroxymethyl)dioxanes have been constructed. in addition, an unusual adenine-substituted divinyl ether (22) was synthesized via a base-catalyzed, double elimination of a 2',3'-di-o-t ... | 1986 | 3023613 |
| beta 2 microglobulin enhances the infectivity of cytomegalovirus and when bound to the virus enables class i hla molecules to be used as a virus receptor. | we have previously demonstrated that human cytomegalovirus (cmv) binds the host protein beta 2 microglobulin (beta 2m) from body fluids or from cell culture media. in this report we have examined the effect of the beta 2m on viral infectivity. we have shown that the addition of human purified beta 2m, or a fraction of foetal calf serum corresponding to bovine beta 2m, to culture medium increased the amount of infectious extracellular cmv, compared to that from cells grown in serum-free medium. m ... | 1987 | 3029306 |
| regional variation in high-density lipoprotein binding to human adipocyte plasma membranes of massively obese subjects. | obesity is associated with significant changes in cholesterol and lipoprotein metabolism. high density lipoprotein (hdl) cholesterol is often reduced and adipose tissue cholesterol stores are increased in obese individuals. this prompted a study on the binding of hld fractions (hdl2 and hdl3) to adipocyte plasma membranes obtained from massively obese subjects (bmi greater than 37 kg m-2) undergoing gastroplasty. regional variation in hdl binding to these adipocyte plasma membranes was demonstra ... | 1987 | 3032643 |
| a high-yield, high-purity elutriation method for preparing human granulocytes demonstrating enhanced experimental lifetimes. | a counterflow centrifugal elutriation procedure for preparing human granulocytes is described. this method permits isolation of 1-2 x 10(9) granulocytes with a yield of 70% +/- 7% from a unit of whole blood in 3 h. these cells are 99% +/- 1% pure and 95-99% viable. when compared to cells prepared by a conventional procedure employing hypotonic lysis to remove red cells, the elutriated cells show enhanced oxidative responses resulting from enhanced longevity. other responses including receptor-me ... | 1987 | 3036986 |
| t and b peripheral blood lymphocytes in normal and lymphocytotic sheep. | surface immunoglobulins (sig), peanut agglutinin (pna), spontaneous erythrocyte rosette (e-rosette) and helix pomatia (hp) marker were investigated in normal and bovine leukemia virus (blv)-infected sheep. in normal sheep, 19.3% +/- 4.9 of peripheral blood lymphocytes (pbl) were sig+, whereas 58% +/- 5.69 were pna+, and 19.6 +/- 5.2 were e-rosette forming cells (e-rfc). in blv-induced lymphocytotic sheep, sig+ cells in pbl reached 59.4% +/- 15.06. in the same animals, pna bound to 20.6% +/- 9.69 ... | 1987 | 3040327 |
| roles of hormonal and nutritional factors in the regulation of rat liver alcohol dehydrogenase activity and ethanol elimination rate in vivo. | fasting reduced the liver alcohol dehydrogenase (adh) activity by 51% (p less than 0.001). insulin, within 2 hr, increased the adh activity found in fasted animals by 28% (p less than 0.02). insulin administration failed to stimulate the reduced adh activity in diabetic rats. however, adh activity in the diabetic-fed rats decreased by 52-54% (p less than 0.001) compared to normal-fed rats regardless of whether they were meal-fed or refed the normal chow. glucagon blocked by 15% (p less than 0.02 ... | 1988 | 3044171 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| metabolism-related tissue-binding of halogenated hydrocarbons. | | 1986 | 3101260 |
| chemotherapy of pulmonary tuberculosis in britain. | | 1988 | 3123192 |
| serum albumin adducts in the molecular epidemiology of aflatoxin carcinogenesis: correlation with aflatoxin b1 intake and urinary excretion of aflatoxin m1. | aflatoxin-serum albumin adducts in the blood of 42 residents of guangxi province, people's republic of china, were determined and compared with intake of aflatoxin b1 (afb1) and excretion of aflatoxin m1 (afm1) in urine. blood specimens were obtained during the same period that urine was collected and that diet was sampled. serum albumin was isolated from blood by affinity chromatography on reactive blue 2-sepharose and subjected to enzymatic proteolysis using pronase. immunoreactive products we ... | 1988 | 3133131 |
| intravenous immune globulin in symptomatic paediatric human immunodeficiency virus infection. | seven paediatric patients with symptomatic human immunodeficiency virus (hiv) infection were prospectively studied for 6-24 months after the start of intravenous immune globulin (ivig) therapy. there was substantial clinical benefit during ivig treatment with marked reduction of febrile and infectious episodes, normalization of physical and psychomotor development, and absence of mortality. the immunologic monitoring revealed some discrete objective improvements. the results of this study favour ... | 1988 | 3134244 |
| smokeless tobacco control. report of a who study group. | | 1988 | 3145646 |
| effects of maternal exposure to polychlorobiphenyls (pcbs) on f1 generation behavior in the rat. | the effect of fenclor 42 (pcb) exposure of female rats (fischer 344 strain) was studied through assessment of the behavioral development of their f1 progeny. female rats were exposed to pcb according to the following treatment schedule: (a) (5 days) 2 weeks prior to mating, (b) during gestation (days 6-15 of pregnancy), (c) during lactation (days 1-21 after delivery). behavioral endpoints of motor reflexes, motor coordination, activity (preweaning behaviors), and learning (postweaning behavior) ... | 1988 | 3146520 |
| clonidine hyperphagia: neuroanatomic substrates and specific function. | recent studies have indicated that the alpha 2-noradrenergic agonist clonidine (clon), when peripherally and centrally administered, potentiates feeding in satiated rats in a manner similar to that observed following injection of norepinephrine (ne) into the hypothalamic paraventricular nucleus (pvn). the present experiments examined the effects of clon on meal patterns and macronutrient selection and compared these findings to earlier ne-stimulated feeding studies. administration of clon (25 nm ... | 1988 | 3147461 |
| distribution of sensory receptors in the flexor carpi radialis muscle of the cat. | the structures and distribution of encapsulated muscle receptors were examined in serial transverse sections of flexor carpi radialis in the adult cat. four types of receptors (muscle spindles, golgi tendon organs, paciniform, and pacinian corpuscles) were identified. their structures resembled those encountered in other limb muscles. pacinian corpuscles were rare and occurred only in the external fascial coat of the muscle near its origin. the other three receptor types were distributed in an u ... | 1985 | 3156250 |
| characterization of protein kinase c and its role in catecholamine secretion from bovine adrenal-medullary cells. | protein kinase c activity towards exogenous histone was detected in a cytosolic fraction of bovine adrenal medulla. the enzyme was dependent on ca2+ and phosphatidylserine for its activity, with half-maximal activation being achieved at approx. 18 microm free ca2+ and 8 micrograms of phosphatidylserine/ml. both diolein and 4 beta-phorbol 12-myristate 13-acetate (tpa) decreased the ca2+ requirement of the enzyme, half-maximal activation being obtained at approx. 12 microm and 9 microm free ca2+ r ... | 1985 | 3159383 |
| structure of the human liver/bone/kidney alkaline phosphatase gene. | in man, there are multiple forms of alkaline phosphatase encoded by at least three homologous genes: placental, intestinal, and liver/bone/kidney. this report describes the characterization of the human liver/bone/kidney alkaline phosphatase locus. the gene appears to exist as a single copy in the haploid genome and is comprised of 12 exons distributed over more than 50 kilobases. in liver, kidney, saos-2 human osteosarcoma cells, and cultured fibroblasts, there is a single major start for trans ... | 1988 | 3165380 |
| [metabolic criteria of viability of isolated cardiomyocytes]. | | 1988 | 3179764 |
| [metabolic criteria of viability of isolated cardiomyocytes]. | | 1988 | 3179764 |
| protein-related differences in the excretion of nitrosoproline and nitrate by the rat--possible modification of de novo nitrate synthesis. | male ola:sd rats were fed purified diets containing 5 or 20% lactalbumin as the source of protein, and the daily urinary excretion of nitrate and nitrosoproline was measured. animals fed the high-protein diet consistently excreted more nitrate and nitrosoproline than littermates fed the low-protein ration, despite a similar, negligible amount of nitrate in both diets. furthermore, whereas nitrite administration enhanced nitrosoproline excretion in both diet groups, nitrate administration increas ... | 1988 | 3220325 |
| determination of u in human tissues by delayed neutron activation analysis. | a rapid, sensitive and highly selective technique using delayed neutron activation analysis (dnaa) has been used to determine u concentrations in human tissues. two different sample preparation techniques were compared: one involves total matrix destruction to a dry ash while the other is a nondestructive preparation of the wet sample. the data obtained from the analyses of the same sample by dnaa of wet tissues, dnaa of ashed tissues and from radiochemical analyses using alpha spectroscopy (a s ... | 1988 | 3198401 |
| epithelium-dependent relaxation of guinea-pig tracheal smooth muscle by carbachol. | modulation of the effect of carbachol by epithelium on tracheal smooth muscle of guinea-pig was studied by using coaxial bioassay system. the contractile response of the bioassay organ (epithelium-free tracheal smooth muscle of guinea-pig) to carbachol was well maintained along the observation period of 30 min. when the same bioassay organ was taken into epithelium-intact tracheal tube of guinea-pig, a significant relaxation followed the carbachol-induced initial contraction within 30 min which ... | 1988 | 3233050 |
| competitive direct enzyme-linked immunosorbent assay for detection of sulfamethazine residues in swine urine and muscle tissue. | a sensitive assay for the detection of sulfamethazine in swine urine and muscle tissue using a direct competitive enzyme-linked immunosorbent assay (elisa) has been developed. undiluted urine or a phosphate-buffered saline extract of pork muscle tissue is mixed with an enzyme-labeled conjugate of sulfamethazine and horseradish peroxidase. the mixture is added to wells of a microtiter plate coated with antibody to sulfamethazine. after the test system is incubated, washed, and re-incubated with s ... | 1988 | 3240969 |
| postnatal brain growth and allometry in the rabbit oryctolagus cuniculus. | a new four-parameter version of pütter's growth curve (pütter, 1920) recently developed by jolicoeur and pirlot (1988) is applied to cross-sectional data on brain and body weight in 66 male rabbits (oryctolagus cuniculus) ranging up to one year in postnatal age. apart from the olfactory bulb, in which there appears to be no initial delay, the estimated initial delays range from 21.7 days (after fertilization) for the cerebellum to 29.9 days for the cerebrum, which suggests that the weight of mos ... | 1988 | 3255724 |
| trimethoprim-sulfamethoxazole for acute dysuria in women: a single-dose or 10-day course. a double-blind, randomized trial. | to compare single-dose and 10-day treatment regimens of trimethoprim-sulfamethoxazole in women with acute dysuria, urgency, or urinary frequency. | 1988 | 3257670 |
| some properties of semicarbazide-sensitive amine oxidases. | the semicarbazide-sensitive amine oxidases (ssaos) comprise a substantial but diffuse group of enzymes separable from classical monoamine oxidase in several respects. differences in cofactor requirement, molecular weight and subcellular distribution are crucial for such a separation. differential sensitivity to enzyme inhibitors, characterized by resistance to inhibition by acetylenic mao inhibitors coupled with sensitivity to semicarbazide and some related compounds are characteristic of these ... | 1987 | 3295116 |
| subendocardial and subepicardial wall thickening during ischemia in exercising dogs. | to determine whether ischemia in the exercising dog is associated with preservation of subepicardial thickening relative to subendocardial thickening, 10 dogs were chronically instrumented with circumflex artery flow probes, hydraulic occluders, and pairs of ultrasonic microcrystals for determination of wall thickness in the circumflex artery distribution. one pair of crystals spanned the entire ventricular wall (transmural), and the other spanned the outer half of the ventricular wall. inner wa ... | 1988 | 3180383 |
| a controlled trial of nebulized aminoglycoside and oral flucloxacillin versus placebo in the outpatient management of children with cystic fibrosis. | six children with cystic fibrosis who had persistently had pseudomonas aeruginosa isolated from their respiratory tract, completed a double-blind cross-over comparison of oral flucloxacillin and nebulized aminoglycoside versus double placebo. the patients had higher fev1 results at the end of the month of active treatment than after the month of placebo. | 1987 | 3329531 |
| recurrent hypercalcemia and elevated 1,25-dihydroxyvitamin d levels in hodgkin's disease. | hypercalcemia has been infrequently associated with hodgkin's disease. when seen, most cases have been attributable to skeletal invasion by disease. herein is described a 40-year-old man with a 15-year history of hodgkin's disease. each of four disease recurrences was heralded by hypercalcemia occurring in the absence of bone disease or elevation of parathyroid hormone levels. marked elevations of 1,25-dihydroxyvitamin d levels were observed that paralleled his disease course and response to the ... | 1988 | 3337120 |
| evaluation of the quality of bacteriologic methods used to diagnose tuberculosis in argentina. | | 1988 | 3147118 |
| pharmacokinetic and pathological evaluation of gentamicin in cats given a small intravenous dose repeatedly for five days. | gentamicin was administered to six cats at a dosage of 3 mg/kg of body weight intravenously every 8 h for five days. peak and trough serum gentamicin concentrations were measured after each injection. gentamicin elimination rate and serum half-life were calculated. serum urea nitrogen, creatinine, biochemistry profile, electrolyte, glucose, total protein, and albumin concentrations were measured daily. urinalyses were performed before and after the five-day experimental period. the mean +/- sd p ... | 1988 | 3370552 |
| bile acid synthesis in man. in vivo activity of the 25-hydroxylation pathway. | during biosynthesis of bile acid, carbons 25-26-27 are removed from the cholesterol side-chain. side-chain oxidation begins either with hydroxylation at the 26-position, in which case the three-carbon fragment is released as propionic acid, or with hydroxylation at the 25-position, in which case the three-carbon fragment is released as acetone. we have previously shown in the rat that the contribution of the 25-hydroxylation pathway can be quantitated in vivo by measuring production of [14c]acet ... | 1988 | 3134400 |
| alveolar macrophage subpopulations' responsiveness to chemotactic stimuli. | recent data suggest that alveolar macrophages are a heterogeneous group of cells with several subpopulations. this study was undertaken to determine if there is heterogeneity among rat alveolar macrophage subpopulations ability to respond to chemotactic stimuli. alveolar macrophages were harvested by bronchoalveolar lavage and fractionated into density-defined fractions by centrifugation through a continuous isoosmotic gradient of colloidal silica. unfractionated and density-defined alveolar mac ... | 1988 | 3394797 |
| follicle-stimulating hormone induces deoxyribonucleic acid polymerase alpha before deoxyribonucleic acid synthesis in immature rat ovarian follicles perfused in vitro. | to examine the effects of follicle-stimulating hormone (fsh) on cell proliferation and cell division in ovaries, deoxyribonucleic acid (dna) polymerase activities and dna concentrations were determined in ovarian follicles of immature, hypophysectomized, estradiol-treated rats perfused in vitro. ovine fsh (2-20 micrograms/ml) significantly enhanced the dna polymerase alpha activity in the follicles perfused for 180-300 minutes, whereas the dna polymerase beta activity and dna concentration showe ... | 1988 | 3126468 |
| the effects of triclabendazole on the lung fluke, paragonimus uterobilateralis in the experimental host sigmodon hispidus. | the new veterinary drug triclabendazole (fasinex) was tested for its anthelmintic activity against adult paragonimus uterobilateralis in experimentally infected cotton rats, sigmodon hispidus. after a single dose of 50, 100 or 150 mg/kg b.w. triclabendazole or after two doses of 5 or 10 mg/kg b.w. given at an interval of 24 hours, 56-61% of the flukes were dead at three to five weeks after treatment. two doses of 50 or 75 mg/kg b.w. triclabendazole killed all flukes. the flukes, which were still ... | 1988 | 3067323 |
| ultrastructure of the sebaceous glands of the camel (camelus dromedarius). | the sebaceous gland of the camel has been studied by light and electron microscopy. the gland is lobed but the lobes are not subdivided into lobules. the ultrastructure of the sebaceous gland of the camel is generally similar to that of other animals. it consists of cells at different stages of development and maturation. the cells which are programmed to produce lipid secretion are probably those which lose contact with the basal lamina. various forms of smooth endoplasmic reticulum are seen in ... | 1988 | 3417542 |
| improved method for determination of plasma pindolol by high-pressure liquid chromatography with ultraviolet detection. | | 1987 | 3427212 |
| analysis of epidermal proteins for dna-binding activity. | a group of dna-binding proteins from the soluble extract of newborn rat epidermis have been separated by chromatography using dna-cellulose columns. the electrophoretogram of the dna-binding proteins eluted from a single stranded dna-cellulose column shows five major proteins of molecular weights ranging between 25k to 40k. both the epidermal protein filaggrin and most keratins, except two high molecular weight keratins, do not show in vitro dna-binding activity. | 1987 | 3427216 |
| [trisomy 13 by translocation. possible prenatal ultrasonic diagnosis]. | | 1988 | 3057974 |
| actions of prostaglandins e2 and f2 alpha on release of 14c-labelled mucins from rat submandibular salivary acini in vitro. | mucin secretion was studied in vitro using well-characterized preparations of isolated acini. pge2 significantly (p less than 0.05) increased release of [14c]-glucosamine labelled mucins at the highest concentration tested (10(-5) m), but was ineffective at lower doses (10(-9)-10(-6) m). pgf2 alpha had no effect on mucin secretion over this concentration range. pge2 (10(-9)-10(-5) m) did not modify isoproterenol stimulated mucin secretion. the cyclooxygenase inhibitor indomethacin (10(-7)-10(-5) ... | 1987 | 3482153 |
| characterization of feline t and b cells. | feline peripheral-blood lymphocyte populations (n = 22) were examined for the following markers: rosette formation with guinea pig erythrocytes (gpe-t cells), rosette formation with human rbc (hrbc-t cells), rosette formation with sheep rbc, mixed rosette formation with gpe-t cells and hrbc-t cells (total t cells), erythrocyte antibody-complement rosettes, and surface immunoglobulin. an average of 28% +/- 7% (range, 16% to 39%) of the feline lymphocytes formed rosettes with gpe-t cells, and 27% ... | 1987 | 3493718 |
| significance of intracellular calcium and cyclic adenosine 3',5'-monophosphate in the mechanisms by which prostaglandins influence human sperm function. | the selective ability of pge-1 and pge-2 to enhance the capacity of human spermatozoa for hamster oocyte penetration was dependent upon high concentrations (1.7 mm) of extracellular ca2+ and a prolonged (4 h) duration of exposure, and insensitive to the ca2+ channel antagonist, verapamil. studies with the intracellular calcium indicator quin-2 indicated that exposure to pge-1 and pge-2, but not pgf-2 alpha, induced a significant rise in the levels of cytoplasmic ca2+, suggesting that an ionophor ... | 1986 | 3016256 |
| effect of dibutyryl cyclic amp on morphologic features and biologic markers of a human salivary gland adenocarcinoma cell line in culture. | dibutyryl cyclic amp (db-camp) has marked effects on the growth, morphologic features, and biologic markers of a human salivary gland adenocarcinoma cell line in culture. a cell line with ultrastructure and biologic markers specific to the intercalated duct cells of human salivary glands was cultivated in the presence of db-camp. major alterations, such as process formation and expression of myofilaments and oxytocin receptor in addition to myosin and s-100 protein, were observed in those cells ... | 1986 | 3002591 |
| polarized plasma membrane domains in cultured endothelial cells. | to determine whether distinct plasma membrane domains exist in endothelial cells, we infected monolayer cultures of macro- and microvascular endothelial cells with enveloped rna viruses known to bud selectively from either the apical or basal surface in polarized epithelial cells. we found that vesicular stomatitis (vsv) and sendai virus emerge asymmetrically from cultured endothelial cells. this provides direct evidence for the existence of polarized plasma membrane domains in vascular endothel ... | 1985 | 2996916 |
| [functional status of the rat insular apparatus and adrenal cortex at early periods after irradiation]. | as early as 0.5-3 h after x-irradiation of rats with a lethal dose of 12 gy glucocorticoid activity of adrenal cortex was enhanced and 11-oxycorticosteroid level in blood was increased. the increasing of the immunoreactive insulin content of blood was registered at the background of hypercorticoidism and normal function of insular apparatus during the first hours following irradiation. at later times (24-72 h), a stable hypercorticoidism developed, the ability of beta-cells to react adequately t ... | 1986 | 3534939 |
| [functional status of the rat insular apparatus and adrenal cortex at early periods after irradiation]. | as early as 0.5-3 h after x-irradiation of rats with a lethal dose of 12 gy glucocorticoid activity of adrenal cortex was enhanced and 11-oxycorticosteroid level in blood was increased. the increasing of the immunoreactive insulin content of blood was registered at the background of hypercorticoidism and normal function of insular apparatus during the first hours following irradiation. at later times (24-72 h), a stable hypercorticoidism developed, the ability of beta-cells to react adequately t ... | 1986 | 3534939 |
| endotoxin-induced bovine mastitis: immunoglobulins, phagocytosis, and effect of flunixin meglumine. | milk whey immunoglobulins (ig) and phagocytosis of staphylococci by milk polymorphonuclear neutrophilic leukocytes (pmn) were measured in 12 cows (allotted to 6 pairs) during acute bovine mastitis induced by intramammary inoculation of endotoxin. six of these cows (or 1 in each pair) were treated with flunixin meglumine and were compared with the others (given only saline solution). the endotoxin inoculation comprised 10 micrograms of escherichia coli o26:b6 lipopolysaccharide injected into one ... | 1986 | 3538954 |
| histochemical and biochemical urease localization in the periplasm and outer membrane of two proteus mirabilis strains. | proteus mirabilis, a gram-negative bacillus, is often implicated in the formation of infectious kidney stones. as ureolytic activity of this organism is thought to play a major role in its pathogenesis, we adapted our recently described urease localization technique to visualize urease activity in vivo. urease activity was ultrastructurally localized in two clinically isolated p. mirabilis strains by precipitating the enzymatic reaction product (ammonia) with sodium tetraphenylboron. subsequent ... | 1986 | 3539291 |
| failure of klebsiella pneumoniae antibodies to cross-react with peripheral blood mononuclear cells from patients with ankylosing spondylitis. | cross-reactivity between antibodies to 2 strains of klebsiella pneumoniae (k43 and f77) and the peripheral blood lymphocytes of patients with ankylosing spondylitis (as) was examined in 3 separate antibody binding and cytotoxicity assays. using k pneumoniae antisera in a chromium release cytotoxicity assay, we found no difference in the reactions of cells from as patients and those from control subjects. this result contrasts with the results of previous studies. similarly, using an enzyme-linke ... | 1987 | 3551965 |
| alterations in concentrations of cyclic guanosine 3',5'-monophosphate in guinea pig urine during the development of a transplantable leukaemia. | cyclic guanosine 3',5'-monophosphate (cgmp) concentrations were measured in urine specimens from guinea pigs before and after transplantation of a leukemia. a transient increase in cgmp concentration occurred 3 days after inoculation which preceded any detectable increase in the white blood cell count. this peak in cgmp concentration was found to be very highly significant when compared with fluctuations in urinary cgmp levels in the guinea pigs from transplantation of the leukemia. inoculation ... | 1985 | 2992765 |
| a stable tracer technique for calcium metabolism studies. | a tracer technique using the stable isotope calcium-48 was developed to facilitate studies of human calcium metabolism. small samples of 1 ml serum were used. calcium levels were determined with good precision using neutron-activation analysis after pre-concentration of the serum calcium by oxalate precipitation. the method has sufficient sensitivity to detect induced dilutions of the tracer by administering other less costly isotopes of calcium. the ability to utilize small volumes of sample is ... | 1988 | 2980818 |
| [cardiac defibrillation. experimental and theoretical aspects]. | | 1987 | 3577372 |
| the polypeptide fold of the globular domain of histone h5 in solution. a study using nuclear magnetic resonance, distance geometry and restrained molecular dynamics. | the polypeptide fold of the 79-residue globular domain of chicken histone h5 (gh5) in solution has been determined by the combined use of distance geometry and restrained molecular dynamics calculations. the structure determination is based on 307 approximate interproton distance restraints derived from n.m.r. measurements. the structure is composed of a core made up of residues 3-18, 23-34, 37-60 and 71-79, and two loops comprising residues 19-22 and 61-70. the structure of the core is well def ... | 1987 | 3608994 |
| abdominal operations: effect on subsequent experimental faecal peritonitis. | rats were subjected to laparotomy, anaesthesia only (controls), laparotomy plus 1-cm-segment ileum isolation, or no treatment (controls) and were challenged with endogenous faecal peritonitis 1 and 2 weeks later, respectively. survival rates were 40% for the laparotomy group and 100% for the ileum isolation group, whereas all controls died. the number of peritoneal leucocytes, particularly macrophages and neutrophils, was significantly (p less than 0.05) higher in the laparotomy group than in th ... | 1987 | 3629184 |
| [a method of quantitative determination of delta 5,7-sterols in unsaponifiable lipid fractions]. | the technique is offer for rapid quantitative definition of sterols having a system of delta 5,7-conjugated double bonds which conditions biological activity of d vitamin--the result of photoisomerisation of the sterols. the technique bases on the analysis of uv absorption spectra of unsaponifiable lipid fraction of several kinds of mutant yeast strains and the model mixtures of sterols. the results obtained concerning the estimation of delta 5,7-sterols containing in total fraction are confirme ... | 1987 | 3658928 |
| adrenergic and cholinergic stimulation of arachidonate and phosphatidate metabolism in cultured astroglial cells. | primary cultures of neonatal rat brain polygonal astroglia, or cells of the c62b glioma line, were incubated with [1-14c]arachidonic acid (aa) in culture for 18 hr. in both culture systems, more than 80% of the added [1-14c]aa was taken up into cellular glycerolipids; less than 1% of the radioactivity in the cells was present in an unesterified form. when prelabeled c62b cells were stimulated with acetylcholine (ach), there was a rapid accumulation of arachidonyl-phosphatidic acid (pa) accompani ... | 1986 | 2875401 |
| bevantolol hydrochloride--preclinical pharmacologic profile. | bevantolol hydrochloride, a beta adrenoceptor antagonist, can be categorized using conventional schemes as being cardioselective, devoid of intrinsic sympathomimetic activity and having weak membrane-stabilizing and local anesthetic properties. the cardioselectivity of bevantolol was conferred by the incorporation of a 3,4-dimethoxyphenyl moiety into the terminal amino portion of the molecule. this portion of the molecule also appears to account for bevantolol's in vitro binding affinity at alph ... | 1986 | 2871786 |
| structural and functional relationships of grafted vasopressin neurons. | fetal vasopressin neurons were grafted into adult long-evans rats with vasopressin deficiencies created by neural lobe ablation one week prior to implantation. control animals and recipients with ectopic grafts still possessed significant deficits in fluid regulation 6 weeks following implantation. however, recipients with grafts fused to the host median eminence and containing magnocellular vasopressin neurons juxtaposed to the host portal vasculature showed restored peripheral vasopressin acti ... | 1986 | 3708331 |
| reciprocal functional interaction of adjacent myocardial segments during regional ischemia: an intraventricular loading phenomenon affecting apparent regional contractile function in the intact heart. | the mechanism of the increase in function in myocardial segments adjacent to those subjected to regional ischemia is not known. to investigate the hypothesis that changes in regional loading are an important causative factor of this phenomenon, 17 dogs were instrumented with segmental shortening sonomicrometers in the left anterior descending and circumflex artery distributions, circumflex coronary flow probes and cuff occluders, as well as circumflex coronary pressure catheters. additionally, t ... | 1986 | 3711491 |
| reciprocal functional interaction of adjacent myocardial segments during regional ischemia: an intraventricular loading phenomenon affecting apparent regional contractile function in the intact heart. | the mechanism of the increase in function in myocardial segments adjacent to those subjected to regional ischemia is not known. to investigate the hypothesis that changes in regional loading are an important causative factor of this phenomenon, 17 dogs were instrumented with segmental shortening sonomicrometers in the left anterior descending and circumflex artery distributions, circumflex coronary flow probes and cuff occluders, as well as circumflex coronary pressure catheters. additionally, t ... | 1986 | 3711491 |
| reevaluation of the role of gangliosides as receptors for tetanus toxin. | binding of tetanus toxin to rat brain membranes was of lower affinity and capacity when binding was determined in 150 mm nacl, 50 mm tris-hcl (ph 7.4) than in 25 mm tris-acetate (ph 6.0). binding under both conditions was reduced by treating the membranes with neuraminidase. pronase treatment, however, reduced toxin binding only in the tris-saline buffer (ph 7.4). in addition, the concentration of gangliosides required to inhibit toxin binding was 100-fold higher in tris-saline compared to tris- ... | 1986 | 3711900 |
| 3,3',5'-triiodothyronine inhibits iodothyronine-5'-deiodinating activity induced by 3,5,3'-triiodothyronine at equimolar concentrations in cultured fetal mouse liver. | to investigate the rt3 effect on iodothyronine-5'-deiodinating activity (i-5'-da) in cultured fetal mouse liver, liver on the 19th day of gestation, in which little or no i-5'-da was detected and, therefore, rt3 was very stable, was cultured in medium containing thyroid hormone-depleted fetal calf serum supplemented with cortisol, insulin, and various concentrations of iodothyronines. after 4-15 days of culture, i-5'-da in the homogenate was assessed by the amount of iodide released from outer r ... | 1986 | 3732155 |
| effect of tick-borne fever on the disposition of sulphadimidine and its metabolites in plasma of goats. | the effect of tick-borne fever (tbf) on the plasma disposition of sulphadimidine (sdm) and its metabolites in goats was studied. in uninfected goats, sdm was extensively metabolised mainly by hydroxylation, glucuronidation and to a minor extent by acetylation. in tbf infected goats the hydroxylation of sdm into 6-methylhydroxysulphadimidine (sch2oh) as well as into 5-hydroxysulphadimidine (soh) was markedly reduced (-57.6 and -63.6 per cent, respectively). an unidentified metabolite (metabolite ... | 1986 | 3738235 |
| baroreceptor-independent medullary mechanism for release of vasopressin during hypotension in rats. | plasma vasopressin (avp) levels were measured at rest (mean arterial pressure 80-85 mmhg) and during hypotension (mean arterial pressure 38-45 mmhg) induced by ganglionic blockade (trimethaphan) in halothane-anaesthetized respirated rats with end-tidal pco2 maintained at 34-40 mmhg. hypotension (15 min) produced a 310% increase in plasma avp (+/- 60% s.e.m.) which was not reduced significantly by prior baro- and chemoreceptor denervation. the hypotension-induced rise in avp was blocked by bilate ... | 1988 | 2842427 |
| [concentration of anti-brain antibodies in the blood of patients with diffuse toxic goiter and thyrotoxic encephalopathy]. | the content of anticerebral antibodies was studied in the peripheral blood of 10 healthy persons, 42 patients with diffuse toxic goiter and 25 patients with thyrotoxic encephalopathy. it was determined by a method of passive hemagglutination using a human brain protein extract as an antigen. in the healthy persons antibodies to cerebral antigens were undetectable whereas in 24 of 42 patients with diffuse toxic goiter without signs of the nervous system involvement they were detected (log2 titer- ... | 1986 | 3754967 |
| in vivo stability of in vitro labelled 99tcm-red blood cells for radionuclide determination of left ventricular ejection fractions and volumes. | serial determination of radionuclide left ventricular ejection fraction and volumes require a label that remains highly stable after introduction into the human circulation. we have evaluated the in vivo stability of 99tcm-red blood cells (99tcm-rbc) labelled in vitro by preparation with small amounts of a stannous agent in 19 patients with coronary artery disease. the distribution volume of 99tcm-rbc was virtually identical to that of rbc labelled with 51cr. labelling efficiency expressed as th ... | 1986 | 3774253 |
| interactive responses to diethylstilboestrol in c3h mice. | female c3heb/fej mice were fed diethylstilboestrol (des) at a dietary concentration of 100 ppb beginning at 6 wk of age. several oestrogen-sensitive target organs (uterus, vagina, bone and mammary gland) were removed and examined histologically when mice died, became moribund, were killed at scheduled intervals or were removed because of presumptive mammary tumours (palpable mass having a 1-cm diameter). oestrogen-sensitive histological observations for which there was less than a 100% prevalenc ... | 1986 | 3781439 |
| [changes and induction of drug-metabolizing enzymes in rats with hepatic injuries]. | | 1986 | 3804171 |
| [vacuous chewing after haloperidol and gaba-linoleamide administration in the rat]. | | 1986 | 3812899 |
| [vacuous chewing after haloperidol and gaba-linoleamide administration in the rat]. | | 1986 | 3812899 |
| [changes in the estrous cycle of the rat after irradiation with a helium-neon laser]. | | 1986 | 3812911 |