| purification and characterization of l-asparaginase with anti-lymphoma activity from vibrio succinogenes. | homogeneols l-asparaginase with anti-lymphoma activity was prepared from vibrio succinogenes, an anaerobic bacterium from the bovine rumen. an overall yield of pure l-asparaginase of 40 to 45% and a specific activity of 200 +/- 2 iu per mg of protein was obtained. the pure enzyme can be stored at -20 degrees for at least 3 months with no loss of activity. the isoelectric point of the l-asparaginase is 8.74. no carbohydrate, phosphorus, tryptophan, disulfide, or sulfhydryl groups were detected. t ... | 1976 | 11211 |
| the covalently bound flavin of vibrio succinogenes succinate dehydrogenase. | | 1977 | 14028 |
| evidence for peptidoglycan-associated protein(s) in neisseria gonorrhoeae. | | 1978 | 27176 |
| pattern of milk flow in breast-fed infants. | 122 six-day-old infants were test-weighed during breast feeding. cross-sectional data collection avoided repeated interruptions of individual feeds. milk flow conformed to a definite pattern: a mean of 50% of the feed from each breast was taken in the first two minutes of sucking and a mean of 80-90% was taken by four minutes. the second half of a ten-minute feed from either breast was almost non-nutritive. preliminary studies suggest that the pattern is the same after one month of lactation. | 1979 | 87962 |
| studies on histamine release induced by compound 48/80 and peptide 401 [proceedings]. | | 1979 | 88890 |
| detection of antilymphocyte antibody with two-color method in systemic lupus erythematosus and its heterogeneous specificities against human t-cell subsets. | the two-color method originally described by van rood et al. (van rood, j. j., a. van leeuwen, and j. s. ploen. 1976. simultaneous detection of two cell populations by two-color fluorescence and application to the recognition of b-cell determinants. nature (lond.). 262: 795-797) for the typing of homologous leukocytic antibodies, d-region was used for the detection of antilymphocyte antibody (ala) in systemic lupus erythematosus. in this method, surface immunoglobulin-bearing cells were identifi ... | 1979 | 91623 |
| the murine kupffer cell. i. characterization of the cell serving accessory function in antigen-specific t cell proliferation. | murine kupffer cells, the tissue macrophages of the liver, were isolated by collagenase digestion, differential sedimentation over metrizamide, and glass adherence. the resultant cell population was more than 86% phagocytic, and 95% of cells stained positively for alpha-naphthyl butyrate esterase activity. the cells also had cell surface receptors for complement (c) and the fc portion of igg. in addition, a large proportion of kupffer cells was shown to bear ia antigens: about half of the cells ... | 1979 | 91637 |
| the specificity of human autoantibodies that react with both cell nuclei and plasma membranes: the nuclear antigen is present on core mononucleosomes. | we have examined the nature of the nuclear antigen recognized by certain natural human antibodies that react specifically with both cell nuclei and plasma membranes from many species. partial purification of these antibodies, called x-ana, is achieved by binding to and rapid elution from the surface of viable human leukocytes. chicken erythrocyte chromatin was solubilized by digestion with staphylococcal nuclease and fractionated into a 0.15 m nacl soluble fraction that consisted of core mononuc ... | 1979 | 91640 |
| purification of human plasma alpha 2 macroglobulin and alpha 1 proteinase inhibitor using zinc chelate chromatography. | | 1979 | 92900 |
| characterization of various horse thyroid forms of cyclic-nucleotide phosphodiesterase [proceedings]. | | 1979 | 92956 |
| comparison of antigen-specific i-region-associated cell interaction factors. | two basic types of factors reacting with anti-i region (anti-ia) antisera are compared, those derived from macrophage-like antigen presenting cells and others derived from t-lymphocytes, of either the suppressor or helper type. despite the common property of reacting with anti-ia antisera, the two sets of factors differ by many criteria. macrophages, upon culture with antigen, release complexes of ia antigen and a fragment of the original immunogen. this material is only produced by responder ma ... | 1979 | 93870 |
| hereditary alpha 2-macroglobulin deficiency. | on screening of a normal material 1 man was found (age 37) who on repeated determinations had a low alpha 2m, namely 25 % with electroimmuno assay according to laurell. investigation of the family revealed that the mother (age 69) and one daughter (age 5) had low values too. all other coagulation and fibrinolytic components were normal. they had no signs of increased fibrinolysis and normal levels of alpha 2-antiplasmin, alpha 1-antitrypsin and antithrombin iii. liver function tests were normal. ... | 1979 | 94459 |
| a biochemical comparison of development of various facial bones in neonatal rats. | | 1979 | 94540 |
| the effect of dextran on the incidence of thrombosis in microvenous nakayama ring pin anastomoses. including further studies on the histopathology of nakayama anastomoses. | anastomoses of the posterior facial veins in the rabbits neck (diameter appr. 1.5-2.0 mm) were performed under the operating microscope, using the nakayama ring pin stapler. immediately before the operation the animals received clinical dextran (mw 70000) or physiological saline intravenously in a double-blind randomized pattern. the total incidence of partial or complete thrombosis in this experiment was low (around 10%). no difference between the two groups were detected. all anastomoses were ... | 1979 | 94697 |
| the effect of glutaurine on the development of amphibian larvae with inhibited rna and protein synthesis. | 1. glutaurine (gamma-l-glutamyl-taurine) isolated from bovine parathyroid and later synthetized retards the metamorphosis of rana arvalis. 2. from day 13 of treatment glutaurine moderates the metamorphosis inhibiting effect of antibiotics acting at the transcription and translation level. | 1979 | 94855 |
| major and modified nucleosides in trna hydrolysates by high-performance liquid chromatography. | we describe a high-performance liquid chromatographic analytical method that can be readily placed in operation, and which is particularly well suited to scientists investigating trna structure, biosynthesis, and function, and for the determination of major and modified nucleosides of trna. the method is characterized by the following features: (1) sensitivity at the nanogram level; (2) high chromatographic resolution and selectivity; (3) direct measurement of nucleosides with accuracy and preci ... | 1979 | 94916 |
| contribution to the knowledge of histophysiological characteristics of the pineal gland of ewes during pregnancy. | | 1979 | 95051 |
| fibrinogen turnover in pregnant rabbits during the first and last thirds of gestation. | although thrombin-mediated fibrinogen derivatives have been observed in normal pregnancy, an increased turnover of fibrinogen has been suspected but has not yet been demonstrated. fibrinogen turnover was studied in 14 pregnant and 10 nonpregnant female rabbits by use of purified rabbit 125i-labeled fibrinogen. in the last third of gestation the half life of fibrinogen was shortened by 45% and the fractional catabolic rate increased by 44% in comparison to the values for the first third of gestat ... | 1978 | 99036 |
| congeners of dom: effect of distribution of the evaluation of pharmacologic data. | | 1978 | 101880 |
| the safety of rapeseed oil. | | 1978 | 101916 |
| restricted posterior parietal lesions in the rhesus monkey and performance on visuospatial tasks. | monkeys with lesions restricted to the inferior parietal lobule or the banks and depths of the superior temporal sulcus were tested on a route-following task. these areas are considered on neuroanatomical grounds to be homologous to parts of the human posterior parietal cortex, where lesions produce profound spatial disorientation. the operated monkeys were impaired on the route task, thus confirming at the behavioural level the anatomical predictions of comparability between parietal cortex in ... | 1979 | 103604 |
| a new sequence-specific endonuclease from anabaena cylindrica. | | 1978 | 103749 |
| domains and the hinge region of an immunoglobulin heavy chain are encoded in separate dna segments. | a 6.8-kilobase dna fragment containing the sequence coding for the constant region of the mouse immunoglobulin gamma1 heavy chain was cloned from total cellular dna. electron microscopic and nucleotide sequencing studies showed that the three protein domains and the hinge region are encoded in separate dna segments. | 1979 | 106304 |
| penicillin-resistant gonorrhea. | | 1979 | 106997 |
| resonance raman spectroscopy of chlorophyll-protein complexes. | resonance raman spectra of chlorophyll a (chl a) and of chl b were selectively obtained, at low temperature, from chlorophyll-protein complexes prepared from green and blue-green algae and from higher plants. antenna chl a in the chl a-p700-protein complexes (cp i) and in the light-harvesting chl a/b-protein complexes (cp ii gives resonance raman spectra extremely close in all their features to those previously obtained from intact cells and chloroplasts. in particular, the same multiplicity of ... | 1978 | 110567 |
| [microbiological quality of frozen vegetable foods. ii. indicator microorganisms]. | | 1979 | 122280 |
| modification of yeast phosphofructokinase by trypsin and subtilisin in the presence of effectors. | yeast phosphofructokinase was subjected to limited proteolysis by trypsin in the presence of different effectors. it could be demonstrated that the substrates mgatp and fructose-6-phosphate are able to protect the enzyme from inactivation by trypsin. other effectors like amp, adp, phosphoenolpyruvate, citrate and ammonium ions exhibit only negligible effects. during the first step of degradation consisting in the conversion of the subunits from mr 120,000 to 90,000 no significant effects of the ... | 1978 | 154818 |
| differential susceptibility to amphotericin b of 3t3 cells transformed by sv40 or polyoma. | | 1975 | 169082 |
| [sympathetic vascular tonus in beta blockade]. | | 1977 | 207039 |
| improved growth media for vibrio succinogenes. | five new culture media for vibrio succinogenes are described. of these, a medium composed of 0.4% yeast extract, 100 mm ammonium formate, 120 mm sodium fumarate, and 0.05% sodium thioglycolate, ph 7.3, supports the best growth. | 1975 | 234152 |
| mechanism of sensitivity of cultured pancreatic carcinoma to asparaginase. | the effects of e. coli l-asparaginase on cultured human pancreatic carcinoma (mia paca-2) have been studied. the enzyme (1 u/ml) inhibited growth and protein synthesis in both mia paca-2 and panc-1, another pancreatic carcinoma cell line, but had little or no effect on human breast carcinoma or melanoma cells. the inhibition of protein synthesis by e. coli l-asparaginase was largely reversed by l-glutamine but not by l-asparagine. the growth of both mia paca-2 and panc-1 showed absolute dependen ... | 1978 | 363626 |
| primary structure of yeast proteinase b inhibitor 2. | the complete amino acid sequence of yeast proteinase b inhibitor 2 (ib2) was determined to be h3n+-thr-lys-asn-phe-ile-val-thr-leu-lys-lys-asn-thr-pro-asp-val-glu-ala-lys-lys-phe-leu-asp-ser-val-his-his-ala-gly-gly-ser-ile-leu-his-glu-phe-asp-ile-ile-lys-gly-tyr-thr-ile-lys-val-pro-asp-val-leu-his-leu-asn-lys-leu-lys-glu-lys-his-asn-asp-val-ile-glu-asn-val-glu-asp-lys-glu-val-his-thr-asn-coo-. elucidation of the primary structure was enabled by automated edman degradation and cooh-terminal hydro ... | 1979 | 387783 |
| comparison of plasma, erythrocyte and brain lithium concentrations in the guinea-pig and rat [proceedings]. | | 1979 | 497586 |
| [response of certain laboratory animals to being fed an ocean fish, the ruvette]. | | 1979 | 516605 |
| [food and biological value of the adapted acidophilous mixtures, maliutka and malysh]. | the new acidophilus liquid mixtures "malyutka" and "malysh" manufactured under industrial conditions were subjected to comprehensive physicochemical, microbiological and biological studies. amino acids and fatty acids contained by the mixtures as well as their biological value were also examined. | 1979 | 516609 |
| [mathematical model of stretch reflex system of single muscle with an eye to the gamma activity (author's transl)]. | | 1979 | 522301 |
| the effects of dopamine on neurones in the nucleus tractus solitarii and on the central control of blood pressure in the rat [proceedings]. | | 1979 | 529063 |
| effect of medium composition on the growth and asparaginase production of vibrio succinogenes. | asparaginase synthesis by vibrio succinogenes is induced by ammonium ions. synthesis occurs throughout exponential phase, and in early stationary phase asparaginase accounts for about 5% of the total soluble protein. the organism grows best when fumarate is provided as the terminal electron acceptor of the formate-oxidizing cytochrome system. yeast extract or enzyme-hydrolyzed proteins are effective nutrient sources. in an ammonium formate-sodium fumarate medium, where maximum growth and asparag ... | 1978 | 697359 |
| clinical isolates of anaerobic gram-negative rods with a formate-fumarate energy metabolism: bacteroides corrodens, vibrio succionogenes, and unidentified strains. | strains of anaerobic, gram-negative bacteria, isolated from human clinical specimens and from studies of human normal flora, that have energy metabolism similar to vibrio succinogenes are described. included are four human isolates of v. succinogenes, five similar strains of motile straight rods, three strains of bacteroides corrodens, and two unidentified strains. all strains studied grew poorly in usual anaerobic broth media but produced good turbidity in overnight broth cultures in media cont ... | 1976 | 770499 |
| hydrogen-dependent organisms from the human gingival crevice resembling vibrio succinogenes. | twenty-eight strains of microaerophilic, motile, slightly curved gram-negative rods isolated from the gingival crevice of patients with gingivitis were studied. they seemed similar to vibrio sputorum, though eleven strains differed in minor characters from bergey's description under the new name campylobacter sputorum, subspecies sputorum. the oral strains studied appeared to be closely related to several species of the genus campylobacter and to vibrio succinogenes. the oral strains were able t ... | 1976 | 821391 |
| ability of insulin to increase calcium binding by adipocyte plasma membranes. | calcium specifically binds to adipocyte plasma membranes, demonstrating two classes of binding sites having affinity constants of 4.5 x 10(4) m-1 and 2.0 x 10(3) m-1. insulin (100 microunits/ml) added directly to the isolated plasma membranes caused no alteration in calcium binding, whereas incubation of the adipocytes with 100 microunits/ml of insulin resulted in a 2.50 +/- 1.6% increase in calcium binding to the subsequently isolated plasma membranes. the increase in calcium binding produced b ... | 1976 | 1064022 |
| comparative antihypertensive effects and tissue distribution of beta adrenergic blocking drugs. | the precise mechanism of the antihypertensive action of beta adrenergic blocking drugs is not known. both peripheral and central sites of action have been proposed. the comparative antihypertensive actions and tissue distribution of propranolol, pindolol and sotalol were investigated in both normotensive sprague-dawley and spontaneously hypertensive rats. from recordings of concurrent changes in blood pressure and heart rate it was observed that oral or subcutaneous administrations of both propr ... | 1975 | 1151751 |
| femoral percutaneous catheterization in infants and small children for cerebral angiography. | an improved technique of selective cerebral angiography for infants and small chicken is described. the needle is inserted approximately 45 degrees to the skin in order to avoid subintimal placement of the tip. after good backflow is established, the guidewire is advanced to a position in the descending aorta. a no. 3 polyethylene catheter and 21-gauge scalp vein needle were most successful. | 1975 | 1153751 |
| the effect of iodine deficiency on thyroid function in the infant rat. | we have studied rats born to severely iodine-deficient mothers and subsequently maintained on a low-iodine diet (lid) from birth to 41 days. they were compared with controls born to mothers fed a high-iodine diet (hid). lid babies from birth on had large goiters, high thyroid labeled mit/dit ratios and radioiodine uptakes, high plasma tsh and low plasma t4 in comparison to hid controls. thyroid labeled t3/t4 ratios were low in all babies at birth but were higher in the lid than hid babies from d ... | 1975 | 1196133 |
| synchronization of the fission yeast schizosaccharomyces pombe using heat shocks. | | 1975 | 1196141 |
| the intercellular content of the three transcobalamins at various stages of normal and leukaemic myleoid cell development. | a study of the three serum transcobalamins (tc) in myeloproliferative disorders and acute leukaemia has suggested a granulocyte origin for the three binders; the later stages of this cell line might be releasing 'tc i' and 'tc iii' while 'tc ii' which is increased in sera from patients with acute myelogenous leukaemia, probably originates at least in part from myeloblasts. the intracellular content of each tc has been studied in normal and leukaemic bone marrow cells after separation on a discon ... | 1975 | 1201243 |
| attenuated, streptomycin-dependent salmonella typhi oral vaccine: potential deleterious effects of lyophilization. | four studies were done with streptomycin-dependent salmonella typhi as an oral, attenuated vaccine. studies 1 and 3 employed freshly harvested vaccine, whereas studies 2 and 4 involved lyophilized vaccine. five to eight doses (3 x 10(10)-10(11) organisms/dose) were given; oral streptomycin (1.0 g) was administered concomitantly in studies 2 and 3, with only two of the doses of vaccine in study 1, and was not given in study 4. no adverse reactions were encountered in 179 vaccinated men, and 94% o ... | 1976 | 1262709 |
| [methods of studying the electrical activity of the stomach and duodenum]. | | 1976 | 1272972 |
| [treatment and monitoring of primary hypothyroidism]. | treatment of primary hypothyroidism requires a long-term hormonal therapy with levothyroxine which restores a normal tsh value. in a series of 192 patients followed up for at least 3 years we tried to evaluate the variability of maintenance dosage and to analyse the factors predictive of replacement dosage. the l-t4 dosage varied from one individual to the other, from 0.6 to 3.2 micrograms/kg. the mean maintenance dosage (118 +/- 38 micrograms/day) was similar in men and women weight for weight, ... | 1992 | 1287777 |
| analysis of cell surface antigens of campylobacter rectus. | campylobacter rectus is one of the predominant bacteria in the lesions of human periodontitis. the surface antigens of the bacterium contain several components which may play significant roles in colonization and pathogenesis. a high-molecular-weight protein was selectively isolated from the cell surface of c. rectus by acid extraction and purified by deae sepharose. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that the extracted protein was 150 kda. the protein was ... | 1992 | 1303324 |
| characterization of the structure and properties of the his 62-->ala and arg 38-->ala mutants of yeast phosphoglycerate kinase: an investigation of the catalytic and activatory sites by site-directed mutagenesis and nmr. | the role of two "basic patch" residues, arg-38 and his-62, in the catalytic function and anion-dependent activation of yeast 3-phosphoglycerate kinase (pgk) was investigated by site-directed mutagenesis. steady-state kinetics and nmr experiments were conducted to characterize the functional properties and structural integrity of the r38a and h62a mutants. the results of these studies, in combination with earlier mutagenesis experiments, suggest that arg-38 is the only catalytically essential res ... | 1992 | 1304916 |
| expression and release of neuroregulators during development: monoamines and neuropeptides. | the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ... | 1992 | 1306802 |
| gap-43 mrna localization in the rat hippocampus ca3 field. | gene expression of the axonal growth-associated protein, gap-43, has been studied in the adult rat brain by in situ hybridization histochemistry. this protein is synthesized at high levels in neuronal somata in immature and regenerating neurons, but after establishment of mature synaptic relations its synthesis generally declines sharply, thus providing a marker denoting propensity for exhibiting synaptic plasticity. detailed examination of the distribution of mrna for gap-43 in rat hippocampus ... | 1992 | 1317499 |
| the pathogenicity of drug-resistant variants of herpes simplex virus. | | 1992 | 1317602 |
| multiple rearrangements and activated expression of c-myc induced by woodchuck hepatitis virus integration in a primary liver tumour. | woodchuck hepatitis virus (whv) is a small, partially double-stranded dna virus. like the related human hepatitis b virus (hbv), whv induces acute and chronic hepatitis and hepatocellular carcinoma (hcc) in its natural host. whv dna integration into c-myc and n-myc, resulting in deregulated expression of these genes, has been described previously in woodchuck hcc. we have analysed a woodchuck liver tumour in which whv dna was integrated in the c-myc gene. the virus insertion provoked multiple al ... | 1992 | 1317604 |
| an immunochemical and immunocytologic study of the increase in myometrial gap junctions (and connexin 43) in rats and humans during pregnancy. | myometrial gap junctions are thought to play a necessary role in control of parturition. in this study we evaluated the presence of gap junctions in myometrial tissues with a specific antibody to the gap junction protein, connexin 43, with immunocytochemistry and immunoblots. immunofluorescence of rat heart showed gap junctions were localized along the intercalated disks, but no change occurred during pregnancy. fluorescent staining of rat myometria showed punctate staining of gap junctions betw ... | 1992 | 1323215 |
| soluble tnf and membrane tnf expressed on cd4+ t lymphocytes differ in their ability to activate macrophage antileishmanial defense. | in our studies of host defense against the intracellular parasite leishmania major, we obtained evidence for a novel mechanism of macrophage activation for antimicrobial defense that involves direct cell contact between cd4+ t lymphocytes and leishmania-infected macrophages. the mechanism is distinctive as it does not involve secretion of lymphokines but is apparently mediated by the membrane-anchored form of tumor necrosis factor (mtnf; approximately 50-60 kd) present on the surface of the effe ... | 1992 | 1347312 |
| isolation and sequence of a cdna encoding the precursor of a bombesinlike peptide from brain and early embryos of xenopus laevis. | a cdna encoding the precursor of a bombesinlike peptide was isolated from brain of xenopus laevis. the predicted end product resembles neuromedin b, which was originally isolated from mammalian spinal cord. the mrna for this precursor was also present in gastrointestinal tract and in ovaries. moreover, it could be detected in early embryos (stage 2 and stage 10) of x. laevis. these findings suggest novel roles for peptides of the bombesin family in oocyte maturation and early amphibian developme ... | 1992 | 1409705 |
| relative effects of intraoperative tissue expansion and undermining on wound closing tensions. | intraoperative tissue expansion is advocated as a technique to facilitate closure of soft-tissue wounds with less tension than if they were closed primarily without expansion or, presumably, simple undermining. few objective studies have been done, however, to validate the technique. we specifically questioned the relative effects of intraoperative tissue expansion and undermining on wound closing tensions in an animal model. our data showed statistically significant reduction in the force requi ... | 1992 | 1418898 |
| targeted cytokine production. | it has been well established that bispecific antibodies containing anti-t-cell receptor mabs crosslinked to anti-tumor mabs induce t cells to lyse tumor cells, as measured in a 51cr-release assay. such lysis requires direct attachment between target and cytotoxic cells and most probably involves the exocytosis of cytolytic substances into the cell:cell interface. in addition, targeted t cells mediate a second activity, the secretion into the medium of factors that can block the growth of bound t ... | 1992 | 1428400 |
| sexual cranial dimorphism in malnourished rats treated with growth hormone. | one hundred and sixty two holtzman rats of both sexes were malnourished during suckling and post-weaning periods. some of them received periodic injections of growth hormone. after sampling, at 56 days of age, skulls were cleaned and measured. differences within and between sexes were estimated by mahalanobis d2 distances. normal cranial differentiation between sexes (scd) was decreased by malnutrition and restored by growth hormone treatment. the effect of the growth hormone on skull size was l ... | 1992 | 1428418 |
| [colchicine in toxic liver cirrhosis and primary biliary cirrhosis]. | in animals, colchicine has been shown hepatoprotective, which is related to its anti-fibrotic and anti-inflammatory effect. in man, colchicine has been used for the treatment of alcoholic liver disease and primary biliary cirrhosis. although controlled trials observed an improvement of liver function tests under colchicine, the survival rate in most studies was only slightly improved without reaching statistical significance. therefore, at present, no final evaluation of colchicine for the treat ... | 1992 | 1449015 |
| volumes of the cytoarchitectonic areas in the rat cerebral cortex. | the volumes of the isocortical, periallocortical and allocortical areas were measured in the brains of nine 6 month old male wistar rats. after perfusion fixation with bodian's solution, determination of brain weight, paraffin embedding and serial sectioning, the areal volumes were estimated with the cavalieri method and corrected for individual brain shrinkage. thus, fresh volumes were measured. the present report provides comprehensive data on the volumes of the cortical areas of one hemispher ... | 1992 | 1479193 |
| the influence of the stomach on crop function and feeding behavior in domestic turkeys. | we tested the hypotheses that motility of the crop and muscular stomach are coordinated and that the stomach exerts primary control over crop filling and emptying in domestic turkeys. simultaneous recordings of motility of the crop, esophagus, and stomach with implanted strain gauge transducers and visual observations of food passage using image intensification radiography revealed an inverse relationship between the frequency of stomach and crop contractions. artificially filling the stomach of ... | 1992 | 1523251 |
| localization and quantification of nonapeptide binding sites in the kidney of xenopus laevis: evidence for the existence of two different nonapeptide receptors. | the distribution and properties of nonapeptide binding sites in the kidney of the anuran xenopus laevis were investigated using quantitative in vitro autoradiography. the binding studies were performed with [3h]arginine vasopressin (avp) as ligand because [125i]arginine vasotocin (avt) lacks biological activity. specific binding sites for [3h]avp are located in the glomeruli of the kidney. [3h]avp binding results in a steady state of association and dissociation between ligand and binding sites. ... | 1992 | 1563620 |
| ab-interno neodymium:yag versus erbium:yag laser sclerostomies in a rabbit model. | this study was undertaken to determine whether thermally-induced tissue necrosis was a factor in ab-interno contact-laser sclerostomy failure. a rabbit model was used to compare the continuous-wave neodymium (nd):yag with the pulsed erbium (er):yag laser with respect to such failure. laser energy was focused into a fused-silica fiber optic (400 microns) for the nd:yag laser (12 w; 3 to 5 seconds), and into a single-crystal, uncladded sapphire fiber optic (250 microns) for the er:yag laser (7 to ... | 1992 | 1574289 |
| the relation of ligand binding to redox state in the hexa-heme nitrite reductase of wolinella succinogenes. | ligand binding reactions and the relation between redox state and ligand binding in the hexa-heme nitrite reductase of wolinella succinogenes have been studied using laser flash photolysis. on a picosecond time scale, a rapid excursion was observed corresponding to the breaking and reforming of an iron histidine bond. with the co derivative, a geminate reaction was observed with a rate of 3 ns-1. on a nanosecond time scale, no slower geminate reactions were observed. for the cyanide derivative, ... | 1992 | 1597437 |
| [the human chorionic gonadotropin (hcg) receptor: a new class within the family of gtp protein coupled receptors. epitope mapping of receptor-bound agonistic and antagonistic forms of hcg]. | human chorionic gonadotropin (hcg) is a member of the glycoprotein hormone family. it is composed of an alpha and a beta subunit, the latter being closely homologous to that of human luteinizing hormone (hlh). hcg and hlh bind to the same receptor. the molecular mass of hcg is 38 kd, up to 30% of which is contributed by the carbohydrate moieties linked to each of the two subunits. structure-function relationship studies have indicated that both subunits interact with the receptor and that altoge ... | 1992 | 1636261 |
| reactivity of the bacillus subtilis succinate dehydrogenase complex with quinones. | the succinate dehydrogenase isolated from bacillus subtilis was found to catalyze the oxidation of succinate with hydrophilic quinones. either naphthoquinones or benzoquinones served as acceptors. the enzyme activity increased with the redox potential of the quinone. the highest turnover number was commensurate with that of the bacterial succinate respiration in vivo. the succinate dehydrogenase was similarly active in fumarate reduction with quinols. the highest activity was obtained with the m ... | 1991 | 1655027 |
| terminal epidermal differentiation of human keratinocytes grown in chemically defined medium on inert filter substrates at the air-liquid interface. | a fully differentiated epithelium having the features of epidermis was obtained in vitro by culturing second-passage normal human keratinocytes (nhk) in the chemically defined medium mcdb 153 on inert filter substrates at the air-liquid interface for 14 d. vertical sections stained for histology and indirect immunofluorescence studies show a correct stratification and expression of differentiation markers. the presence of desmosomes, keratohyalin granules, and lamellar granules, and the formatio ... | 1990 | 1698886 |
| phylogeny of helicobacter felis sp. nov., helicobacter mustelae, and related bacteria. | strain cs1t (t = type strain) is a gram-negative, microaerophilic, urease-positive, spiral-shaped bacterium that was isolated from the gastric mucosa of a cat. additional strains which possessed biochemical and ultrastructural characteristics similar to those of strain cs1t were isolated from the gastric mucosa of cats and dogs. the guanine-plus-cytosine content of the dna of strain cs1t was 42.5 mol%. the 16s rrna sequences of strain cs1t, strain ds3 (a spiral-shaped isolate from a dog), and he ... | 1991 | 1704791 |
| revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
| atypical structure of the 23s ribosomal rna molecule in certain oral bacteria. | ribosomal rna (rrna) isolated from wolinella recta and seven related bacteria was examined by agarose gel electrophoresis. the 23s rrna molecule could not be detected in w. recta, wolinella curva, bacteroides gracilis, or bacteroides ureolyticus. in place of the 23s molecule, there were three smaller molecules of approximately 1700, 650, and 600 bases designated 23s alpha, 23s beta, and 23s delta, respectively. an intact 23s rrna molecule could be isolated from wolinella succinogenes, campylobac ... | 1991 | 1710629 |
| direct polymerase chain reaction test for detection of helicobacter pylori in humans and animals. | we designed a polymerase chain reaction (pcr) for amplifying the helicobacter pylori gene encoding 16s rrna. primers for the specific detection of h. pylori were designed for areas of the 16s rrna gene in which there is the least sequence homology between h. pylori and its closest relatives. the specificity of detection was confirmed by ensuring that the primers did not amplify dna extracts from the campylobacters h. cinaedi, h. mustelae, and wolinella succinogenes, which are the closest relativ ... | 1991 | 1723072 |
| helicobacter muridarum sp. nov., a microaerophilic helical bacterium with a novel ultrastructure isolated from the intestinal mucosa of rodents. | helical organisms with novel ultrastructural characteristics were isolated from the intestinal mucosa of rats and mice. these bacteria were characterized by the presence of 9 to 11 periplasmic fibers which appeared as concentric helical ridges on the surface of each cell. the cells were motile with a rapid corkscrewlike motion and had bipolar tufts of 10 to 14 sheathed flagella. the bacteria were microaerophilic, nutritionally fastidious, and physiologically similar to helicobacter species and w ... | 1992 | 1736969 |
| analysis of high psa n-cam expression during mammalian spinal cord and peripheral nervous system development. | using a monoclonal antibody that recognizes specifically a high polysialylated form of n-cam (high psa n-cam), the temporal and spatial expression of this molecule was studied in developing spinal cord and neural crest derivatives of mouse truncal region. temporal expression was analyzed on immunoblots of spinal cord and dorsal root ganglia (drgs) extracts microdissected at different developmental stages. analysis of the ratio of high psa n-cam to total n-cam indicated that sialylation and desia ... | 1991 | 1769342 |
| cloning and nucleotide sequence of the psra gene of wolinella succinogenes polysulphide reductase. | the polysulphide reductase (formerly sulphur reductase) of wolinella succinogenes is a component of the phosphorylative electron transport system with polysulphide as the terminal acceptor. using an antiserum raised against the major subunit (psra, 85 kda) of the enzyme, the corresponding gene (psra) was cloned from a lambda-gene bank. the n-terminal amino acid sequence of psra mapped within the psra gene product, which also contained an apparent signal peptide. downstream of the psra gene two m ... | 1992 | 1597189 |
| urinary metabolites of doxapram in premature neonates. | 1. urine samples from 20 premature neonates who received doxapram by i.v. infusion were analysed for drug metabolites by g.l.c-mass spectrometry. 2. in addition to doxapram, all urines contained at least one metabolite, but the known metabolite, 3-ketodoxapram, was detected in only 50% of the samples, and in some instances only in trace amounts. 3. significant inter-individual differences in the metabolic pathways of doxapram were observed. 4. a total of six metabolites of doxapram were isolated ... | 1991 | 1796617 |
| the immunobiology of mycoplasma arthritidis and its superantigen mam. | | 1991 | 1802617 |
| the immunobiology of mycoplasma arthritidis and its superantigen mam. | | 1991 | 1802617 |
| the quinone-reactive ni/fe-hydrogenase of wolinella succinogenes. | the hydrogenase (hyd) isolated from the cytoplasmic membrane of wolinella succinogenes consists of three polypeptides (hyda, hydb and hydc) and contains cytochrome b (6.4 mumol/g protein), which was reduced upon the addition of h2. the enzyme catalyzed the reduction of 2,3-dimethyl-1,4-naphthoquinone with h2, in contrast to an earlier preparation which was made up of hyda and hydb only and did not contain cytochrome b (unden, g., böcher, r., knecht, j. & kröger, a. (1982) febs lett. 145, 230-234 ... | 1992 | 1587288 |
| interaction of aminoalkylcarbamates of forskolin with adenylyl cyclase: synthesis of an iodinated derivative of forskolin with high affinity for adenylyl cyclase. | 7-(2-aminoethyl)aminocarbonyl-7-desacetylforskolin (7-aec-fsk) and 6-(2-aminoethyl)aminocarbonylforskolin (6-aec-fsk) were synthesized and tested for their ability to activate adenylyl cyclase and inhibit the high affinity binding of [3h]forskolin to bovine brain membranes. forskolin and 7-aec-fsk were equipotent in activating adenylyl cyclase, with ec50 values of about 4 microm, whereas 6-aec-fsk had an ec50 of about 2 microm. 6-aec-fsk and 7-aec-fsk stimulated adenylyl cyclase about 7-fold ove ... | 1992 | 1538712 |
| k+ channel blocking and anti-muscarinic effects of a novel piperazine derivative, ino 2628, on the isolated dog atrium. | the effects of a novel piperazine derivative, ino 2628, on the negative inotropic and chronotropic responses to intracardiac parasympathetic nerve stimulation and carbachol, to adenosine and to the k+ channel openers, pinacidil and nicorandil, were investigated in isolated, blood-perfused dog heart preparations. ino 2628 (0.1-10 mumol) injected into the sinus node artery of the isolated atrium induced negative chronotropic and small positive inotropic responses in a dose-dependent manner. ino 26 ... | 1991 | 1828769 |
| effects of fluorescein isothiocyanate on insulin actions in rat adipocytes. | the effects of fluorescein isothiocyanate ii (fitc) on the actions of insulin in rat adipocytes were studied. when adipocytes were incubated with fitc at ph 7.4 (2 mm agent, 8 min), the cells were completely deprived of their specific insulin-binding activity and rendered unresponsive to the hormone. the effect of fitc on the insulin-binding activity was milder at ph 9.0, and camp phosphodiesterase in cells exposed to fitc at ph 9.0 was maximally stimulated if the insulin concentration was incre ... | 1992 | 1536560 |
| an apparently allosteric effect involving n2o with the nitrous oxide reductase from wolinella succinogenes. | it was shown that kcat for the benzyl viologen cation (bv+)-n2o oxidoreductase activity of nitrous oxide reductase from wolinella succinogenes was 2-3 times greater at high n2o concentrations than at low. this effect of n2o on kcat exhibited a titration curve implicating a single secondary binding site for n2o with a kd of 130-200 microm (km with respect to n2o is about 2.5 microm). this work represents the first evidence of an apparently allosteric kinetic effect among nitrous oxide reductases. ... | 1992 | 1520293 |
| [effect of anp on estradiol and progesterone production by rat ovarian cells]. | isolated ovarian corpus luteal cells and granulosa cells of rat were employed to investigate the effect of alpha-anp on the secretion of progesterone and estradiol. the contents of the steroid hormones are determined by ria. the results showed that 0.1-10 ng/ml anp promoted progesterone production in a dose dependent manner. alpha-anp also enhanced progesterone production by granulosa cells, but not estradiol. it seems that the effect of alpha-anp on ovarian steroidogenesis is a direct one. | 1991 | 1839085 |
| electron paramagnetic resonance observations on the cytochrome c-containing nitrous oxide reductase from wolinella succinogenes. | nitrous oxide reductase from wolinella succinogenes, an enzyme containing one heme c and four cu atoms/subunit of mr = 88,000, was studied by electron paramagnetic resonance (epr) at 9.2 ghz from 6 to 80 k. in the oxidized state, low spin ferric cytochrome c was observed with gz = 3.10 and an axial cu resonance was observed with g parallel = 2.17 and g perpendicular = 2.035. no signals were detected at g values greater than 3.10. for the cu resonance, six hyperfine lines each were observed in th ... | 1991 | 1846617 |
| growth arrest induced by transforming growth factor beta 1 is accompanied by protein phosphatase activation in human keratinocytes. | protein phosphorylation and dephosphorylation are involved in regulation of cell growth. we tested the hypothesis that the growth inhibitory effect of transforming growth factor beta 1 (tgf-beta 1) involves activation of protein phosphatases. exposure of human keratinocytes in culture to 400 pm tgf-beta 1 for 48 h led to 80% inhibition of dna synthesis as measured by nuclear labeling. incubation of cultured keratinocytes with 400 pm tgf-beta 1 rapidly activated (within 30 min) protein serine/thr ... | 1991 | 1847373 |
| miniature manipulator with microelectrode for chronic recording of single units from freely-moving animals. | we report a simple recording and stimulating system, a miniature manipulator with a microelectrode (mmm) that can be mounted on animal's skull. the system consists of a miniature manipulator and a microelectrode which are used in one body. several mmms can be implanted simultaneously into the brain as the part of the manipulator is small. with this device, recording of single units from several different cortices is possible from awake and unrestricted animals. | 1991 | 1851979 |
| sleep organization and energy expenditure of breast-fed and formula-fed infants. | sleep organization of infants may be influenced by differences in nutrient intakes from human milk and formula. because sleep/awake and sleep stage patterns affect energy expenditure, we hypothesized that differences in sleep organization between breast-fed and formula-fed infants might account in part for differences in energy expenditure between feeding groups. sleep stages and cycling of 4-mo-old breast-fed (n = 10) formula-fed (n = 10) infants were studied with simultaneous measurements of e ... | 1992 | 1480450 |
| comparison of the indirect immunobead, radiolabeled, and immunofluorescence assays for immunoglobulin g serum antibodies to human sperm. | the relative sensitivities of the indirect immunobead test, the indirect flo cytometric immunofluorescence assay, and an indirect radiolabeled antiglobulin assay were compared. eighteen immunobead test positive sera and 18 negative sera were used as the standard for the other two assays. of the 18 positive sera, 14 (77%) and 5 (27%) were positive in the immunofluorescence assay and the radiolabeled antiglobulin assay, respectively. four (22%) of the low titer immunobead test positive sera were n ... | 1991 | 1899399 |
| diabetes-induced rat hyposensitivity to compound 48/80. | rat mortality and contractile responses of isolated tracheas to compound 48/80 from rats made diabetic 4 days before by a single intravenous injection of alloxan and from diabetic rats that had been treated with insulin 6 h before were compared with control animals. diabetic animals and tracheal segments from diabetic rats were significantly less responsive to compound 48/80 than control and insulin-treated diabetic animals. on the other hand, diabetic animals have a lower quantity of peritoneal ... | 1991 | 1913334 |
| dissociated loss of vibration, joint position and discriminatory tactile senses in disease of spinal cord and brain. | the clinical functions of the posterior columns of the spinal cord and the signs of disease of these structures have been debated for years. todd in 1847 and schiff in 1858 knew the functions of the posterior columns and 10 years later brown-séquard knew as well. reynolds, romberg, and duchenne, each described a posterior column syndrome based on a disease in which the primary lesion was not in the posterior columns. in the last 150 years almost every white matter structure of the cord has been ... | 1991 | 1913366 |
| polycythemia and steroid overproduction in a gonadotropin-secreting seminoma of the testis. | while investigating the cause of mild polycythemia in a young man, a testicular seminoma was discovered with unusual and tumor-dependent features: an absolute polycythemia with high plasma erythropoietin (epo) levels, an overproduction of estradiol and testosterone, and a dramatic leydig cell hyperplasia surrounding the tumor tissue. the authors attempted to gain insight into the relationship between this testicular tumor and the hormonal overproduction, i.e., of epo, estradiol, and testosterone ... | 1991 | 1913460 |
| location of the basal disk and a ringlike cytoplasmic structure, two additional structures of the flagellar apparatus of wolinella succinogenes. | the basal body of wolinella succinogenes consists of a central rod, a set of two rings (l and p rings), a basal disk from 70 to 200 nm in diameter, and a terminal knob. in negatively stained preparations of flagellar hook-basal body complexes, some disks remain fixed perpendicularly to the grid and show that such a disk is located on the distal side of the p ring. the basal disks have been isolated with and without the p ring; in both cases there is a hole in the center of the disk. the diameter ... | 1992 | 1370283 |
| [creatinine clearance as the foundation for clinical determination of kidney function in swine]. | comparative examinations of renal function using inulin- and endogenous creatinine-clearance tests in 5 pigs showed nearly identical values of glomerular filtration rate (gfr). based on this relation 79 healthy pigs, weighting 2 kg to 230 kg were investigated using the creatinine-clearance test for determination of normal values of gfr and urine flow rate (vu) as well as renal excretion (e), renal clearance (clr) and fractional excretion (fe) of urea, sodium, potassium, calcium, phosphorus, gluc ... | 1991 | 1948984 |
| isolation and biochemical and molecular analyses of a species-specific protein antigen from the gastric pathogen helicobacter pylori. | a protein of mr 26,000 which was present in large quantities in extracts of cells of helicobacter pylori was purified to homogeneity by ammonium sulfate precipitation followed by gel filtration and reversed-phase chromatography or anion-exchange chromatography. the protein appeared to be associated with the soluble fraction of the cell, and antibodies raised against the protein were reactive with whole-cell lysates of a variety of h. pylori strains in a simple immunodot blot assay. this reaction ... | 1991 | 1987145 |
| modulation of adhesion molecules on human large granular lymphocytes by interleukin-2 in vivo and in vitro. | the modulation of adhesion molecules on human large granular lymphocytes (lgl) by interleukin (il)-2 was investigated both in vivo and in vitro. intercellular adhesion molecule-1 (icam-1; cd54) expression increased on lgl of cancer patients receiving il-2 adoptive immunotherapy. icam-1 expression on lgl isolated by percoll gradient centrifugation, lgl purified, and expanded by adherence to plastic surfaces and lgl identified by leu 19 (cd56) monoclonal antibody were increased significantly in re ... | 1992 | 1347485 |
| multivariate analyses of cellular fatty acids in bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. | the genera bacteroides, wolinella, and campylobacter contain several similar species that require taxonomic revision. fatty acid profiles of whole bacterial cells have proven useful for taxonomy. in this study, cellular fatty acids from bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. were identified and quantitated by gas chromatography and gas chromatography-mass spectrometry, and the data were subjected to principal component analyses. bacteroides fragilis, the type s ... | 1991 | 1993755 |
| construction and characterization of a single polypeptide chain containing two enzymatically active dihydrofolate reductase domains. | a single polypeptide chain containing two dihydrofolate reductase (dhfr) sequences from escherichia coli was constructed to determine if a repeat sequence fusion protein could be expressed in an active form. the possibility that intersequence interactions could play a significant role for this enzyme is suggested by the results of hall and frieden (1989, proc. natl acad. sci. usa, 86, 3060-3064) who observed a substantial decrease in the yield of active enzyme when folded in the presence of a la ... | 1992 | 1287660 |