| development and characterization of porphyromonas gingivalis-specific rat t-cell clones. | porphyromonas gingivalis has been implicated as a major pathogen in periodontitis. to determine the role of t cells in the regulation of this disease, a method was developed for the generation and characterization of rat t-cell clones with antigen specificity to p. gingivalis whole cells. the clones studied so far demonstrated a t-helper (th) phenotype w3/13+, w3/25+, ox8- and ox22-. these t-cell clones proliferated in vitro in response to p. gingivalis, but not to other bacteria (prevotella int ... | 1992 | 1281976 |
| analysis of cell surface antigens of campylobacter rectus. | campylobacter rectus is one of the predominant bacteria in the lesions of human periodontitis. the surface antigens of the bacterium contain several components which may play significant roles in colonization and pathogenesis. a high-molecular-weight protein was selectively isolated from the cell surface of c. rectus by acid extraction and purified by deae sepharose. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that the extracted protein was 150 kda. the protein was ... | 1992 | 1303324 |
| microbiology of healthy and diseased periodontal sites in poorly controlled insulin dependent diabetics. | a group of poorly-controlled insulin dependent diabetes mellitus (iddm) patients were examined in a cross-sectional design for total microbial levels, microbial incidence, and the percent levels of selected periodontal microorganisms. these organisms were selected on the basis of prior reports that associated them with either periodontal disease or health. one periodontally-healthy and one periodontally-diseased site were examined in each iddm patient. increased levels of the periodontal pathoge ... | 1992 | 1315389 |
| the use of dna probes to examine the distribution of subgingival species in subjects with different levels of periodontal destruction. | the present investigation examined the distribution of 14 subgingival species at a total of 2299 sites in 90 subjects with different levels of periodontal destruction. subgingival plaque samples taken from the mesial aspect of each tooth were anaerobically dispersed, diluted and plated on non selective media. after anaerobic incubation, colonies were lifted to nylon filters and specific species detected using digoxygenin-labeled whole chromosomal dna probes. the mean total viable count for all s ... | 1992 | 1318331 |
| humoral igg antibodies to oral microbiota in a population at risk for root-surface caries. | mutans streptococci have been strongly implicated in the initiation of dental caries on coronal surfaces. their role in development of root-surface caries is less clear. the etiologic agents of both types of dental caries are likely to elicit systemic immune responses. the objective of the present study, therefore, was to study the association of clinical variables of disease with humoral igg antibodies to nine oral micro-organisms in 314 adult subjects, aged 45-65 years, who were at risk for ro ... | 1992 | 1321176 |
| subgingival temperature (iii). relation to microbial counts. | the present investigation examined the relationship of selected bacterial species and subgingival temperature. 35 subjects were measured at 6 sites per tooth for clinical parameters and subgingival temperature. measurements were repeated for 21 subjects at 2 month intervals providing a total of 66 subjects visits. at each visit, subgingival plaque samples were taken from the mesial aspect of each tooth and anaerobically dispersed, diluted and plated on non-selective media. after anaerobic incuba ... | 1992 | 1321846 |
| synergistic effect of pyrophosphate and sodium dodecyl sulfate on periodontal pathogens. | our previous studies have shown that pyrophosphate (ppi), the anticalculus component of tartar-control dentifrices, inhibits the growth of organisms associated with coronal and root surface caries. the purposes of this investigation were to: 1) determine if periodontal pathogens are similarly susceptible to the growth-inhibitory properties of ppi; and 2) determine if combinations of pyrophosphate-sodium dodecyl sulfate (ppi-sds) inhibit growth synergistically. porphyromonas gingivalis, actinobac ... | 1992 | 1324304 |
| supragingival cleaning 3 times a week. the microbiological effects in moderately deep pockets. | it is generally believed that supragingival plaque control has little effect on the subgingival microflora of deep periodontal pockets. however, this may not be true for moderately deep pockets (4-5 mm), which may represent a pathological state between gingivitis and marginal periodontitis. in 6 patients with poor oral hygiene and severe gingival inflammation, 4 matched sites (1 in each quadrant), were chosen which demonstrated at least 20% spirochetes and 15% black-pigmented gram-negative bacil ... | 1992 | 1325484 |
| microbial etiology of periodontal diseases. where are we? where are we going? | many studies indicate a strong association of actinobacillus actinomycetemcomitans with localized juvenile periodontitis. species associated with adult periodontitis include bacteroides forsythus, porphyromonas gingivalis, prevotella intermedia, a. actinomycetemcomitans, and wolinella recta. capnocytophaga species may be important in pubertal gingivitis. an unnamed spirochete related to treponema pallidum has been identified in acute necrotizing ulcerative gingivitis lesions. species isolated fr ... | 1992 | 1325846 |
| cytochrome composition and oxygen-dependent respiration-driven proton translocation in wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis. | the membrane fractions of the microaerobically grown type strains of wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis contained membrane-bound cytochrome b, cytochrome c, and co-binding cytochrome c. soluble cytochrome c and co-binding cytochrome c were also present. although b. gracilis is oxidase negative, it possessed cytochrome c. with h2 or formate as the electron donor, proton efflux from anaerobic cells occurred upon addition of a pulse of oxygen. with f ... | 1992 | 1325865 |
| comparative in-vitro activity of azithromycin, macrolides (erythromycin, clarithromycin and spiramycin) and streptogramin rp 59500 against oral organisms. | the in-vitro activities of azithromycin, clarithromycin, spiramycin and rp 59500 were compared with erythromycin against a wide range of oral organisms which have been implicated in oral infections and/or endocarditis (clindamycin was included for oral streptococci). all compounds tested showed good activity against many of these organisms, although some variation was observed with different species. clarithromycin was the most active of the antibiotics tested against gram-positive anaerobes, in ... | 1992 | 1331019 |
| putative periodontopathogens in "diseased" and "non-diseased" persons exhibiting poor oral hygiene. | the aim of the study was to assess the occurrence of some putative periodonto-pathogens in "test" and "control" sites in "diseased" and "non-diseased" persons, respectively, from an adult rural kenyan population exhibiting poor oral hygiene and widespread loss of attachment (la). 14 persons (less than 35 years) were assigned to a "diseased" category on the basis of at least 4 sites with la greater than or equal to 4 mm; at least 5 mm la and a pocket greater than or equal to 4 mm interproximally ... | 1992 | 1346265 |
| immunoglobulins in milk from cows immunized with oral strains of actinomyces, prevotella, porphyromonas, and fusobacterium. | immunization of pregnant cows with bacteria leads to the presence of high concentrations of specific antibodies in colostrum and milk. a total of 14 cows was immunized with single strains of heat-killed oral bacteria or pools of strains of actinomyces, porphyromonas, prevotella, and fusobacterium. two cows were treated with adjuvant alone. the mean percentages of igg1, igg2, igm, and iga in all of the milks were 83.8, 3.8, 9.3, and 3.1, respectively. elisa and whole cell agglutination assays dem ... | 1992 | 1354674 |
| location of the basal disk and a ringlike cytoplasmic structure, two additional structures of the flagellar apparatus of wolinella succinogenes. | the basal body of wolinella succinogenes consists of a central rod, a set of two rings (l and p rings), a basal disk from 70 to 200 nm in diameter, and a terminal knob. in negatively stained preparations of flagellar hook-basal body complexes, some disks remain fixed perpendicularly to the grid and show that such a disk is located on the distal side of the p ring. the basal disks have been isolated with and without the p ring; in both cases there is a hole in the center of the disk. the diameter ... | 1992 | 1370283 |
| pore-forming ability of major outer membrane proteins from wolinella recta atcc 33238. | three major outer membrane proteins with apparent molecular masses of 43, 45, and 51 kda were purified from wolinella recta atcc 33238, and their pore-forming abilities were determined by the black lipid bilayer method. the non-heat-modifiable 45-kda protein (omp 45) showed no pore-forming activity even at high kcl concentrations. the single-channel conductances in 1 m kcl of the heat-modifiable proteins with apparent molecular masses of 43 kda (omp 43) and 51 kda (omp 51) were 0.49 and 0.60 ns, ... | 1992 | 1370429 |
| specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction. | development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ... | 1992 | 1400961 |
| longitudinal monitoring of humoral antibody in subjects with destructive periodontal diseases. | fifty-one subjects (16-61 years old) with evidence of prior destructive periodontal disease were monitored clinically and immunologically at bi-monthly intervals for up to 5 yr. periodontal disease activity, determined as new attachment loss, was detected in 33 of these subjects. only 4 of 51 subjects failed to show an elevated serum antibody level to any of the 18 subgingival species tested. the antibody level threshold established for periodontally healthy subjects was exceeded most often in d ... | 1992 | 1403580 |
| microbiota associated with residual clefts and neighboring teeth in patients with cleft lip, alveolus, and palate. | twenty patients with residual clefts or pronounced soft tissue grooves, treated for uni- or bilateral cleft lip, alveolus, and palate were included in this study. ten patients were recalled for dental prophylaxis at regular intervals, 10 patients were not. one microbiologic sample was obtained from the cleft area and two samples from a tooth adjacent to the cleft (sites adjacent and distant to the cleft). between the recall and the nonrecall group there were notable differences in the presence o ... | 1992 | 1472527 |
| review of chemosystematics: multivariate approaches to oral bacteria and yeasts. | there are several problems related to the classification and identification of bacterial and yeast species assigned to the genera actinobacillus, haemophilus, pasteurella, bacteroides, prevotella, porphyromonas, campylobacter, wolinella, treponema, candida, torulopsis, and saccharomyces, most of which belong to the resident oral microflora. the present review was written to demonstrate how multivariate analyses of data on cellular fatty acids, sugars, enzyme activities, and lysis kinetics during ... | 1992 | 1476049 |
| susceptibility of anaerobic microorganisms to hypothiocyanite produced by lactoperoxidase. | the susceptibility of capnocytophaga ochracea, eikenella corrodens, eubacterium yurii, fusobacterium nucleatum, peptostreptococcus micros, prevotella intermedia, selenomonas sputigena, wolinella recta to hypothiocyanite (oscn-) produced by the lactoperoxidase system was tested. results showed a decrease of bacterial survival rate after oscn- exposure, with an intra- and inter-species variability from 0 to 95% for c. ochracea, 34-100% for e. corrodens, 0-83% for e. yurii, 1-15% for f. nucleatum, ... | 1992 | 1481764 |
| associations between microbial species in dental root canal infections. | the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ... | 1992 | 1494447 |
| periodontal pathogens in the shallow pockets of immigrants from developing countries. | the aim of this study was to examine the distribution of typical periodontitis-associated microorganisms in refugees arriving from non-industrialized countries, and to relate the presence of these organisms to the periodontal condition of the subjects. thirty males between 35-44 years of age were surveyed. dental plaque, calculus, gingivitis, loss of attachment, and probing depths were recorded for all surfaces. a total of 90 microbiological samples were taken with paper points from mesial sites ... | 1992 | 1494449 |
| an apparently allosteric effect involving n2o with the nitrous oxide reductase from wolinella succinogenes. | it was shown that kcat for the benzyl viologen cation (bv+)-n2o oxidoreductase activity of nitrous oxide reductase from wolinella succinogenes was 2-3 times greater at high n2o concentrations than at low. this effect of n2o on kcat exhibited a titration curve implicating a single secondary binding site for n2o with a kd of 130-200 microm (km with respect to n2o is about 2.5 microm). this work represents the first evidence of an apparently allosteric kinetic effect among nitrous oxide reductases. ... | 1992 | 1520293 |
| enumeration of subgingival species on primary isolation plates using colony lifts. | this study evaluated the feasibility of using a colony lift method and dna probes to enumerate bacterial species cultured on primary isolation plates. fourteen digoxigenin-labeled whole chromosomal dna probes representing 12 subgingival species were validated by hybridization with colony lifts prepared from 249 reference strains of 51 species grown on trypticase soy agar plates supplemented with 5% sheep blood. colonies of reference strains were lifted onto nytran filters from plates and treated ... | 1992 | 1528619 |
| the early colonization of gram-negative anaerobic bacteria in edentulous infants. | the occurrence of oral gram-negative anaerobes was examined in 30 edentulous infants (mean age 3 months, range 1-7 months). one pooled swab sample from mucosal surfaces (cheeks, palate, tongue) and one saliva sample was taken from each infant. the samples were cultured aerobically and anaerobically using non-selective and selective media. prevotella (bacteroides) melaninogenica was the most frequently isolated anaerobe, found in 70% of the infants. the other common anaerobes were fusobacterium n ... | 1992 | 1528621 |
| predominant obligate anaerobes in human periodontal pockets. | this study was carried out to investigate the predominant anaerobic bacteria of periodontal pockets in patients with advanced periodontitis, who had no previous treatment other than supragingival scaling, no history of recent or chronic systemic illness, nor any intake of antibiotics within 6 weeks prior to bacteriological sampling. care was taken not to ignore tiny-colony-forming anaerobes, by means of a stereoscope and an anaerobic glove box system. out of 422 (100%) isolates, 380 (90%) were o ... | 1992 | 1531505 |
| wolinella recta in adult gingivitis and periodontitis. | wolinella recta has been associated with adult periodontitis, but its role in the disease remains uncertain. this report clarifies the distribution of w. recta in periodontally healthy and diseased subjects, and treated patients with recurrent disease. a specific polyclonal rabbit antiserum against w. recta strain 372 was used for indirect immunofluorescence localization of w. recta in dental plaque from untreated and treated patients. supragingival plaque was collected from 15 periodontally hea ... | 1992 | 1531512 |
| the quinone-reactive ni/fe-hydrogenase of wolinella succinogenes. | the hydrogenase (hyd) isolated from the cytoplasmic membrane of wolinella succinogenes consists of three polypeptides (hyda, hydb and hydc) and contains cytochrome b (6.4 mumol/g protein), which was reduced upon the addition of h2. the enzyme catalyzed the reduction of 2,3-dimethyl-1,4-naphthoquinone with h2, in contrast to an earlier preparation which was made up of hyda and hydb only and did not contain cytochrome b (unden, g., böcher, r., knecht, j. & kröger, a. (1982) febs lett. 145, 230-234 ... | 1992 | 1587288 |
| cloning and nucleotide sequence of the psra gene of wolinella succinogenes polysulphide reductase. | the polysulphide reductase (formerly sulphur reductase) of wolinella succinogenes is a component of the phosphorylative electron transport system with polysulphide as the terminal acceptor. using an antiserum raised against the major subunit (psra, 85 kda) of the enzyme, the corresponding gene (psra) was cloned from a lambda-gene bank. the n-terminal amino acid sequence of psra mapped within the psra gene product, which also contained an apparent signal peptide. downstream of the psra gene two m ... | 1992 | 1597189 |
| the relation of ligand binding to redox state in the hexa-heme nitrite reductase of wolinella succinogenes. | ligand binding reactions and the relation between redox state and ligand binding in the hexa-heme nitrite reductase of wolinella succinogenes have been studied using laser flash photolysis. on a picosecond time scale, a rapid excursion was observed corresponding to the breaking and reforming of an iron histidine bond. with the co derivative, a geminate reaction was observed with a rate of 3 ns-1. on a nanosecond time scale, no slower geminate reactions were observed. for the cyanide derivative, ... | 1992 | 1597437 |
| modulation of human neutrophil adherence by oral bacteria. | polymorphonuclear neutrophils (pmns) comprise over 90 per cent of leukocytes in the oral cavity. although these phagocytic cells have primary defence roles in the gingiva, their stimulation by micro-organisms may also cause substantial tissue damage due to the release of lysosomal enzymes and oxygen radicals. adherence of pmns to the endothelium and their subsequent diapedesis and egress to areas of infection are considered early vital events in the inflammatory process. in this study, oral bact ... | 1992 | 1605750 |
| reactivity of the bacillus subtilis succinate dehydrogenase complex with quinones. | the succinate dehydrogenase isolated from bacillus subtilis was found to catalyze the oxidation of succinate with hydrophilic quinones. either naphthoquinones or benzoquinones served as acceptors. the enzyme activity increased with the redox potential of the quinone. the highest turnover number was commensurate with that of the bacterial succinate respiration in vivo. the succinate dehydrogenase was similarly active in fumarate reduction with quinols. the highest activity was obtained with the m ... | 1991 | 1655027 |
| relation of baseline microbial parameters to future periodontal attachment loss. | the relationship between the level of subgingival species at baseline and subsequent attachment loss in a subject was examined. 38 subjects (14-71 years) with prior evidence of periodontal destruction were monitored 2x for pocket depth and attachment level at 6 sites per tooth at baseline and 2 months. a subject was considered to exhibit new attachment loss if 1 or more sites increased 3 mm or more in attachment level in 2 months. subgingival plaque samples were taken at the baseline visit from ... | 1991 | 1661304 |
| restriction fragment length polymorphisms in the ribosomal genes for species identification and subtyping of aerotolerant campylobacter species. | whole-cell chromosomal digests of 84 strains of aerotolerant campylobacter (ac) were examined by using pvuii restriction fragment length polymorphisms of rrna genes followed by hybridization with escherichia coli 16s and 23s rrna (ribotyping). the ac strains belonged to campylobacter cryaerophila (n = 13) and a newly defined species, "c. butzleri" (n = 64). strains of c. cryaerophila belonged to two hybridization groups: dna group 1a (including the type strain of c. cryaerophila) and dna group 1 ... | 1991 | 1684797 |
| the relationship of serum igg antibody titers to periodontal pathogens to indicators of the host response in crevicular fluid. | in this study; the relationship of indicators of the local host response in gingival crevicular fluid (gcf) to the serum antibody titer to periodontal pathogens was examined. 15 patients with chronic adult periodontitis were studied. gcf was collected and analyzed for the total amount of igg, igm, the lysosomal enzyme b-glucuronidase (bg) and alpha-2-macroglobulin (alpha 2m). at the same examination, serum from these patients was collected, and enzyme-linked immunosorbent assays used to determin ... | 1990 | 1696949 |
| phylogeny of helicobacter felis sp. nov., helicobacter mustelae, and related bacteria. | strain cs1t (t = type strain) is a gram-negative, microaerophilic, urease-positive, spiral-shaped bacterium that was isolated from the gastric mucosa of a cat. additional strains which possessed biochemical and ultrastructural characteristics similar to those of strain cs1t were isolated from the gastric mucosa of cats and dogs. the guanine-plus-cytosine content of the dna of strain cs1t was 42.5 mol%. the 16s rrna sequences of strain cs1t, strain ds3 (a spiral-shaped isolate from a dog), and he ... | 1991 | 1704791 |
| revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
| atypical structure of the 23s ribosomal rna molecule in certain oral bacteria. | ribosomal rna (rrna) isolated from wolinella recta and seven related bacteria was examined by agarose gel electrophoresis. the 23s rrna molecule could not be detected in w. recta, wolinella curva, bacteroides gracilis, or bacteroides ureolyticus. in place of the 23s molecule, there were three smaller molecules of approximately 1700, 650, and 600 bases designated 23s alpha, 23s beta, and 23s delta, respectively. an intact 23s rrna molecule could be isolated from wolinella succinogenes, campylobac ... | 1991 | 1710629 |
| detection of helicobacter pylori in stomach tissue by use of a monoclonal antibody. | monoclonal antibodies were produced against an acid glycine extract of helicobacter pylori atcc 43504t. one of these appeared to be specific for h. pylori; it recognized all h. pylori isolates by an indirect immunofluorescence assay (iif) but it did not cross-react with the other strains tested, including different species of the genera helicobacter, campylobacter, and wolinella. different strains of members of the families enterobacteriaceae and pseudomonadaceae or other gram-negative bacteria ... | 1991 | 1721923 |
| direct polymerase chain reaction test for detection of helicobacter pylori in humans and animals. | we designed a polymerase chain reaction (pcr) for amplifying the helicobacter pylori gene encoding 16s rrna. primers for the specific detection of h. pylori were designed for areas of the 16s rrna gene in which there is the least sequence homology between h. pylori and its closest relatives. the specificity of detection was confirmed by ensuring that the primers did not amplify dna extracts from the campylobacters h. cinaedi, h. mustelae, and wolinella succinogenes, which are the closest relativ ... | 1991 | 1723072 |
| evolutionary relationships among sulfur- and iron-oxidizing eubacteria. | some 37 reverse transcriptase, partial 16s rrna sequences from sulfur- and/or iron-oxidizing eubacteria, including sequences from species of the genera thiobacillus, thiothrix, thiomicrospira, acidophilium, "leptospirillum," thiovulum, and chlorobium, have been determined. in addition, 16s sequences from a number of unnamed sulfur- and/or iron-oxidizing bacteria from hydrothermal vent sites, from invertebrate-bacterial endosymbioses, and from various mineral recovery operations also have been de ... | 1992 | 1729214 |
| helicobacter muridarum sp. nov., a microaerophilic helical bacterium with a novel ultrastructure isolated from the intestinal mucosa of rodents. | helical organisms with novel ultrastructural characteristics were isolated from the intestinal mucosa of rats and mice. these bacteria were characterized by the presence of 9 to 11 periplasmic fibers which appeared as concentric helical ridges on the surface of each cell. the cells were motile with a rapid corkscrewlike motion and had bipolar tufts of 10 to 14 sheathed flagella. the bacteria were microaerophilic, nutritionally fastidious, and physiologically similar to helicobacter species and w ... | 1992 | 1736969 |
| the microflora of periodontal sites showing active destructive progression. | 20 adult periodontitis (ap) subjects were examined every 2 to 4 months and microbiological samples were collected and cultured when 2 mm or more loss of attachment (active sites) was detected by 2 examiners. similar sites in which no progressive destruction was observed (control sites) also were sampled in the same subjects. by lambda-analysis, there was no statistically significant difference in floras of active (42 sites from 12 subjects) and control (36 sites from 12 subjects) sites or betwee ... | 1991 | 1752997 |
| cloning and nucleotide sequence of the structural genes encoding the formate dehydrogenase of wolinella succinogenes. | the formate dehydrogenase of wolinella succinogenes is a membraneous molybdo-enzyme which is involved in phosphorylative electron transport. the gene (fdha) encoding the largest subunit was isolated from a gene bank by immunological screening. the fdha gene was located in an apparent transcriptional unit (fdha,b,c,d) together with three more structural genes. the n-terminal sequences of three polypeptides present in the isolated enzyme were found to map within the fdha, b and c structural genes. ... | 1991 | 1781728 |
| antimicrobial activity of flurbiprofen and ibuprofen in vitro against six common periodontal pathogens. | nonsteroidal antiinflammatory drugs (nsaids) such as flurbiprofen and ibuprofen, have been shown to inhibit the inflammation and alveolar bone loss associated with chronic destructive periodontal disease. however, the direct effect of nsaids on the gingival crevice microflora has not been studied. the purpose of this investigation was to evaluate the antimicrobial activity of ibuprofen and flurbiprofen in vitro on six commonly isolated periodontal pathogens. the bacterial strains evaluated were ... | 1991 | 1812907 |
| multicenter evaluation of tetracycline fiber therapy. iii. microbiological response. | in a multicenter study of the effects of tetracycline (tc) fiber therapy, subgingival plaque samples were tested for 6 probable periodontal pathogens by dna probe analysis. levels of actinobacillus actinomycetemcomitans, eikenella corrodens, fusobacterium nucleatum, porphyromonas (bacteroides) gingivalis, prevotella intermedia (bacteroides intermedius), and wolinella recta were quantitatively determined in samples taken at baseline, and immediately after tc fiber removal, control fiber removal, ... | 1991 | 1832457 |
| tetracycline fiber therapy monitored by dna probe and cultural methods. | oligonucleotide dna probe and selective cultural methods were compared in their ability to monitor 6 putative periodontal pathogens in a study evaluating local tetracycline fiber therapy. subgingival plaque was sampled from 4 sites in each of 20 subjects. samples were taken before and after therapy from sites assigned to the following test groups: tetracycline (tc) fiber, scaling and root planing, control fiber, and untreated. each sample was analyzed by both dna probe and cultural methods. tota ... | 1991 | 1832458 |
| electron paramagnetic resonance observations on the cytochrome c-containing nitrous oxide reductase from wolinella succinogenes. | nitrous oxide reductase from wolinella succinogenes, an enzyme containing one heme c and four cu atoms/subunit of mr = 88,000, was studied by electron paramagnetic resonance (epr) at 9.2 ghz from 6 to 80 k. in the oxidized state, low spin ferric cytochrome c was observed with gz = 3.10 and an axial cu resonance was observed with g parallel = 2.17 and g perpendicular = 2.035. no signals were detected at g values greater than 3.10. for the cu resonance, six hyperfine lines each were observed in th ... | 1991 | 1846617 |
| wolinella recta, wolinella curva, bacteroides ureolyticus, and bacteroides gracilis are microaerophiles, not anaerobes. | although the nonfermentative, asaccharolytic, putative anaerobes wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis are phylogenetically related to the true campylobacters, the type strains of these species exhibited o2-dependent microaerophilic growth in brucella broth and on brucella agar. the optimum o2 levels for growth of these strains ranged from 4 to 14% in brucella broth and from 2 to 8% on brucella agar, when h2 was provided as the electron donor. no gro ... | 1991 | 1854636 |
| virulence of wolinella recta in a murine abscess model. | the virulence of wolinella recta isolates was studied in an experimental animal model by using monoinfection of balb/c mice. infection with clinical isolates of w. recta 576 and w. recta 234 induced dry, flat, depressed gangrenous necrotic skin lesions, whereas w. recta atcc 33238 failed to induce a similar lesion. histological examination of the skin lesion 72 h postinfection revealed coagulation necrosis of the epidermis, subcutis and cutaneous truncus muscle, with marked exudation of serum pr ... | 1991 | 1855996 |
| dental bacterial plaques. nature and role in periodontal disease. | antony van leeuwenhoek first described oral bacteria. however, not until almost 200 years later was the famous koch postulate introduced. since then, research has extensively been performed regarding the development and microbiology of dental plaques. in spite of the complexity of the developing flora of supragingival plaque, culture studies have shown a remarkably orderly succession of organisms. lately, the concept of microbial specificity in the etiology of periodontal diseases has been widel ... | 1991 | 1890226 |
| extraction, purification, and characterization of major outer membrane proteins from wolinella recta atcc 33238. | the outer membrane of wolinella recta atcc 33238 was isolated by french pressure cell disruption and differential centrifugation. outer membrane proteins (omps) were solubilized by zwittergent 3.14 extraction and separated by deae-sephacel ion-exchange chromatography. the major omps that were found in w. recta atcc 33238 and in several other wolinella spp. consisted of proteins with apparent molecular masses of 51, 45, and 43 kda. these three conserved proteins were purified to essential homogen ... | 1991 | 1894372 |
| microbial aspects of failing osseointegrated dental implants in humans. | the subgingival microflora of 18 failing implants were examined for pathogenic periodontal microorganisms. peptostreptococcus micros was recovered from 6 failing implants, wolinella recta from 6, fusobacterium species from 5, candida albicans from 5, and bacteroides intermedius from 4. enteric rods or pseudomonads constituted a significant part of the microflora in 5 failing implants. actinobacillus actinomycetemcomitans, nonpigmented bacteroides species, capnocytophage species, and staphylococc ... | 1991 | 1906537 |
| factors in virulence expression and their role in periodontal disease pathogenesis. | the classic progression of the development of periodontitis with its associated formation of an inflammatory lesion is characterized by a highly reproducible microbiological progression of a gram-positive microbiota to a highly pathogenic gram-negative one. while this gram-negative microbiota is estimated to consist of at least 300 different microbial species, it appears to consist of a very limited number of microbial species that are involved in the destruction of periodontal diseases. among t ... | 1991 | 1912148 |
| comparative study of lipopolysaccharides from wolinella recta, w. curva, w. succinogenes and campylobacter sputorum ssp. sputorum. | lipopolysaccharides (lps) were extracted from cells of wolinella recta atcc 33238, w. curva atcc 33224, w. succinogenes atcc 29543 and campylobacter sputorum ssp. sputorum a 3563 by a hot phenol-water method and purified by nuclease treatment and by repeated ultracentrifugation. chemical compositions of the purified lps including fatty acid and sugar composition were examined and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) was performed. all lps preparations contained a ... | 1991 | 1916228 |
| monoclonal antibody to a specific antigen from wolinella recta atcc 33238. | four monoclonal antibodies (m-abs) to a specific antigen (150-kda protein antigen) isolated from wolinella recta atcc 33238 by acid extraction were obtained. the four antibodies were all of the igg1 subclass and exhibited equally high specificity for the antigen. a battery of 14 strains or oral bacteria were screened for cross-reactivity with each mab by elisa and western blot analysis. weak cross-reactivity to some strains was observed which differed depending upon the mab. immuno-electromicros ... | 1991 | 1936935 |
| bacteroides ureolyticus (ntu) medium for the selective recovery of bacteroides gracilis. | bacteroides gracilis is a gram-negative anaerobic bacillus which requires formate and fumarate for growth; it has been implicated in periodontal disease and serious infections of the head and neck. in this study, bacteroides ureolyticus (ntu) medium was tested for its ability to allow the growth of b. gracilis and other formate-fumarate requiring gram-negative anaerobes and to enable the recovery of these organisms from clinical specimens. all reference strains grew on ntu medium with the except ... | 1991 | 1942000 |
| analysis of in vitro lymphoproliferative responses and antibody formation following subcutaneous injection of actinobacillus actinomycetemcomitans and wolinella recta in a murine model. | the potential of wolinella recta and actinobacillus actinomycetemcomitans to cause abscesses and induce an immune response was tested in balb/c mice. mice were injected subcutaneously with w. recta, a. actinomycetemcomitans or a mixture of these 2 microorganisms. mice injected with a. actinomycetemcomitans alone, or with both organisms, demonstrated abscesses at the injection site 2 days later, from which pure cultures of a. actinomycetemcomitans were isolated. mice injected with w. recta had sm ... | 1991 | 1945478 |
| dna probe detection of periodontal pathogens in hiv-associated periodontal lesions. | recent studies have shown that an atypical gingivitis and a rapidly progressive periodontal disease may be early-occurring opportunistic infections associated with human immunodeficiency virus (hiv) infection. this study examined the prevalence of selected periodontal pathogens associated with these hiv-related periodontal lesions. subgingival plaque samples were obtained from both hiv-seronegative and hiv-seropositive homosexual men and from presumably uninfected heterosexual men. dna probes we ... | 1991 | 1945481 |
| aspartate and asparagine as electron acceptors for wolinella recta. | since fumarate and nitrate are not usually available in the oral ecosystem, it was investigated whether aspartate and asparagine could be used as alternative electron acceptors by wolinella recta, which is strictly dependent on a respiratory metabolism with formate or h2 as electron donors. both aspartate and asparagine were indeed shown to support growth of w. recta with formate as electron donor. fermentative growth with aspartate alone was not possible. succinate was the major end-product and ... | 1991 | 1945491 |
| dna probe detection of eikenella corrodens, wolinella recta and fusobacterium nucleatum in subgingival plaque. | this cross-sectional study used species-specific dna probes to examine subgingival plaque specimens for the presence of eikenella corrodens, wolinella recta, and fusobacterium nucleatum in adults with untreated periodontitis or gingivitis and in healthy controls. w. recta and f. nucleatum were more prevalent in diseased sites from the periodontitis group when compared with the controls (81% vs 22% and 83% vs 20% respectively). e. corrodens was detected in 62% of the control sites and 81% of the ... | 1991 | 1945492 |
| isolation and biochemical and molecular analyses of a species-specific protein antigen from the gastric pathogen helicobacter pylori. | a protein of mr 26,000 which was present in large quantities in extracts of cells of helicobacter pylori was purified to homogeneity by ammonium sulfate precipitation followed by gel filtration and reversed-phase chromatography or anion-exchange chromatography. the protein appeared to be associated with the soluble fraction of the cell, and antibodies raised against the protein were reactive with whole-cell lysates of a variety of h. pylori strains in a simple immunodot blot assay. this reaction ... | 1991 | 1987145 |
| multivariate analyses of cellular fatty acids in bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. | the genera bacteroides, wolinella, and campylobacter contain several similar species that require taxonomic revision. fatty acid profiles of whole bacterial cells have proven useful for taxonomy. in this study, cellular fatty acids from bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. were identified and quantitated by gas chromatography and gas chromatography-mass spectrometry, and the data were subjected to principal component analyses. bacteroides fragilis, the type s ... | 1991 | 1993755 |
| the structure of the dihaem cytochrome b of fumarate reductase in wolinella succinogenes: circular dichroism and sequence analysis studies. | the fumarate reductase from wolinella succinogenes contains two haem groups with markedly different midpoint potentials (-20 mv and -200 mv). the enzyme is made up of three subunits, the lipophilic one of which (cytochrome b) ligates the haems. circular dichroism (cd) spectroscopy has been applied to the reductase in order to obtain information on the structure of the haems and of their environment. this approach is integrated with amino acid sequence comparison of the cytochrome b with other qu ... | 1991 | 2001380 |
| microbiological study of hiv-related periodontitis. | the subgingival microbiota in 14 persons with hiv-periodontitis was examined. subgingival plaque samples were collected with paper points, transported in vmga iii, and plated on anaerobic enriched brucella blood agar and various selective media. hiv-periodontitis sites revealed actinobacillus actinomycetemcomitans, wolinella recta, peptostreptococcus micros, and bacteroides intermedius, each averaging 7% to 16% of the cultivable subgingival flora in positive patients. high levels of spirochetes ... | 1991 | 2002434 |
| three-dimensional structure of the surface layer of wolinella recta. | the three-dimensional structure of the crystalline surface layer (s-layer) of wolinella recta atcc 33238t, a gram-negative, anaerobic periodontopathogen, was determined to 3.8 nm resolution by electron microscopy and digital image processing. the s-layer protein is closely associated with the outer bacterial membrane, and shows p6 symmetry with lattice spacing and thickness of 21 nm and 15 nm, respectively. the funnel-shaped subunits consist of 6 heavy domains located round a common base at the ... | 1990 | 2080071 |
| effect of a triclosan/copolymer/fluoride dentifrice on the oral microflora. | eighty-one human subjects completed a double-blind study which examined the effects of a 0.3% triclosan/2% gantrez copolymer/0.243% sodium fluoride dentifrice on the microflora of supragingival dental plaque. subjects were divided into an experimental group which performed normal oral hygiene with the triclosan/copolymer/fluoride dentifrice and a control group which also performed normal oral hygiene with the same dentifrice minus the triclosan/copolymer. at baseline, 10 weeks, and 28 weeks, sup ... | 1990 | 2083042 |
| monoclonal antibodies for the detection of 'periodontopathic' bacteria. | the development of indirect immunofluorescence assays using mabs as specific probes has made it possible to detect a variety of suspected periodontal pathogens in subgingival plaque, with exquisite sensitivity. the studies demonstrate high reproducibility of the results if different mabs are used to assess the same bacterial species. bacteroides forsythus, bact. gingivalis, bact. intermedius and wolinella recta, but not actinobacillus actinomycetemcomitans were found in sites of adult periodonti ... | 1990 | 2088218 |
| hydrophobicities of human polymorphonuclear leukocytes and oral bacteroides and porphyromonas spp., wolinella recta, and eubacterium yurii with special reference to bacterial surface structures. | the hydrophobicities of human polymorphonuclear leukocytes (pmnls) and bacteroides buccae, b. oris, b. oralis, b. veroralis, b. buccalis, b. heparinolyticus, b. intermedius, b. denticola, b. loescheii, b. melaninogenicus, porphyromonas gingivalis, p. endodontalis, wolinella recta, and eubacterium yurii were studied by the hexadecane method. the majority of the strains were equally or less hydrophobic than the pmnls. only in the case of e. yurii and the only strain of b. buccalis were all strains ... | 1990 | 2091243 |
| effect of actinobacillus actinomycetemcomitans, wolinella recta and bacteroides gingivalis on the viability of retinoic acid-induced and dimethyl sulfoxide-induced hl-60 cells. | we studied the interactions between viable and heat-killed, opsonized and unopsonized periodontopathic bacteria with both uninduced and induced hl-60 promyelocytic leukemia cells. the cells were induced to differentiate into granulocyte-like cells by incubation with retinoic acid (ra) or dimethyl sulfoxide (dmso). when unopsonized, wolinella recta atcc 33228 significantly suppressed the net proliferation of uninduced hl-60 cells, actinobacillus actinomycetemcomitans strain y4 was markedly lethal ... | 1990 | 2098697 |
| interaction of gram-negative periodontal pathogens with retinoic acid-induced and dimethyl sulfoxide-induced hl-60 cells. | as a first step toward elucidating the reasons for differences among periodontal pathogens in their cytotoxic effects on hl-60 cells, we used transmission electron microscopy to examine morphological aspects of granulocyte-bacteria interactions. unopsonized actinobacillus actinomycetemcomitans strain y4 and bacteroides gingivalis atcc 33277 adhered to, and were phagocytosed by, retinoic acid-induced and dimethyl sulfoxide-induced hl-60 cells. in contrast, there was only minimal interaction betwe ... | 1990 | 2098698 |
| sialidase (neuraminidase) activity among gram-negative anaerobic and capnophilic bacteria. | a filter paper spot test with 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid as a substrate was used to study the prevalence of sialidase activity among gram-negative anaerobic and capnophilic bacteria. a total of 567 isolates representing four genera of obligate anaerobes and four genera of capnophilic organisms was tested. sialidase activity was detected in 94% of 66 isolates from the bacteroides fragilis group, 98% of 66 b. bivius isolates, and all isolates of the following species ... | 1990 | 2108991 |
| studies of the subgingival microflora in patients with acquired immunodeficiency syndrome. | two unique forms of periodontal disease, hiv-gingivitis and hiv-periodontitis, have been described in patients with acquired immunodeficiency syndrome (aids). in order to determine the bacterial species associated with periodontitis in aids patients, the predominant cultivable microflora was examined in 21 subgingival plaque samples from 11 aids patients with periodontitis. the presence of putative periodontal pathogens including actinobacillus actinomycetemcomitans, bacteroides intermedius, por ... | 1990 | 2123926 |
| spectroscopic studies of partially reduced forms of wolinella succinogenes nitrite reductase. | reductive titrations of the dissimilatory hexa-haem nitrite reductase, wolinella succinogenes, with methyl viologen semiquinone (mv) and sodium dithionite, have been followed at room temperature by absorption, natural (cd) and magnetic circular dichroism (mcd) spectroscopies and at liquid helium temperature by electron paramagnetic resonance (epr) and mcd spectroscopies. the nature of the reduced enzyme depends on the reductant employed. at room temperature a single high-spin ferrous haem, obser ... | 1990 | 2162787 |
| the effect of haem ligands on the redox states of the hexa-haem nitrite reductase from wolinella succinogenes. | the nitrite reductase of wolinella succinogenes containing six covalently bound haem groups has one haem group that will not reduce fully in the presence of excess na2s2o4. the effect of the extrinsic ligands co and cyanide on the redox state of this haem was studied by e.p.r. and magnetic c.d. spectroscopy. it was found that both ligands increased the extent of reduction of this haem group, and that in the case of co binding the level of reduction was correlated with the extent of co saturation ... | 1990 | 2171500 |
| two structurally and kinetically distinct forms of wolinella succinogenes nitrite reductase. | it is shown that the oxidized form of the hexa-haem nitrite reductase of wolinella succinogenes exists in two structurally and functionally distinct forms, termed 'resting' and 'redox-cycled'. the nitrite reductase as initially isolated, termed 'resting', has five low-spin ferrihaem groups and one high-spin ferrihaem group. the reduction of these haem groups by na2s2o4 occurs in two kinetically and spectrally distinct phases. in the slower phase the haem groups are reduced by dithionite with a l ... | 1990 | 2171501 |
| susceptibility of oral bacteria to phenoxyethanol and phenoxyethanol/chlorhexidine combinations. | a total of 57 bacterial strains (26 different species) which may be isolated from subgingival plaque were tested for their in vitro susceptibility to phenoxycthanol, a commonly-used antiseptic and preservative. ninety-five percent of the strains, including those associated with chronic inflammatory periodontal disease, were susceptible to concentrations of phenoxyethanol used topically (2% w/v). phenoxyethanol at a concentration of 1% (w/v) was also found that to have a rapid bactericidal effect ... | 1990 | 2202809 |
| plaque and chronic inflammatory periodontal disease. a question of ecology. | the nature of the relationship between dental plaque and chronic inflammatory periodontal disease (cipd) remains unclear, although there is no doubt that plaque is the direct cause. non-specific, specific and exogenous hypotheses have been proposed to explain plaque-host relationships. current evidence indicates that plaque is part of the natural human microflora, one of many such in nature, and that disruption of oral microbial ecology, due primarily to diet texture changes, leads to gingivitis ... | 1990 | 2212083 |
| evaluation of the indoxyl acetate hydrolysis test for rapid differentiation of campylobacter, helicobacter, and wolinella species. | a total of 410 well-defined campylobacter, helicobacter, and wolinella strains, comprising 26 named species, subspecies, and defined groups, were tested for indoxyl acetate hydrolysis by a disk method by using disks prepared at the centers for disease control, atlanta, ga. all c. coli (43 strains), c. cryaerophila (34 strains), c. fennelliae (5 strains), c. fennelliae-campylobacter-like organism 3 (2 strains), c. jejuni (66 strains), c. jejuni subsp. doylei (3 strains), hippurate-negative c. jej ... | 1990 | 2229360 |
| an analysis of the reaction kinetics of the hexahaem nitrite reductase of the anaerobic rumen bacterium wolinella succinogenes. | the reduction kinetics of both the resting and redox-cycled forms of the nitrite reductase from the anaerobic rumen bacterium wolinella succinogenes were studied by stopped-flow reaction techniques. single-turnover reduction of the enzyme by dithionite occurs in two kinetic phases for both forms of the enzyme. when the resting form of the enzyme is subjected to a single-turnover reduction by dithionite, the slower of the two kinetic phases exhibits a hyperbolic dependence of the rate constant on ... | 1990 | 2241924 |
| the fumarate reductase operon of wolinella succinogenes. sequence and expression of the frda and frdb genes. | the genes of the fumarate reductase of wolinella succinogenes are organized in an operon. the three structural genes in the order frdc, frda, frdb, are preceded by a common promoter (körtner et al. 1990) and followed by a terminator of transcription. the proteins encoded by the genes are identical with the subunits present in the isolated enzyme. frda and frdb are hydrophilic proteins consisting of 656 and 238 amino acids, respectively. the 12 cysteine residues present in frdb form 3 ferredoxin- ... | 1990 | 2244791 |
| regulation of the hexaheme nitrite/nitric oxide reductase of desulfovibrio desulfuricans, wolinella succinogenes and escherichia coli. a mass spectrometric study. | dissimilatory nitrite reduction, carried out by hexaheme proteins, gives ammonia as the final product. representatives of this enzyme group from 3 bacterial species can also reduce no to either ammonia or n2o. the redox regulation of the nitrite/nitric oxide activities is discussed in the context of the denitrifying pathway. | 1990 | 2265715 |
| obligately anaerobic bacterial species isolated from foot-rot lesions in goats. | lesions showing clinical signs of foot-rot from 120 goats were cultured on six selective media during october 1987 to november 1988. a total of 582 strictly anaerobic microorganisms belonging to 50 different species was isolated and identified. the anaerobes most frequently isolated belonged to the following genera: bacteroides (80%), peptostreptococcus (63.6%), megasphaera (40%), fusobacterium (29.2%), clostridium (22.5%), propionibacterium (12.5%), eubacterium (11.7%) and leptotrichia (10.8%). ... | 1990 | 2271912 |
| associations between six dna probe-detected periodontal bacteria and alveolar bone loss and other clinical signs of periodontitis. | the purpose of the present study was to assess the associations between the presence and amounts of actinobacillus actinomycetemcomitans, bacteroides gingivalis, b. intermedius, eikenella corrodens, wolinella recta, and fusobacterium nucleatum in the periodontal pocket and the degree of alveolar bone loss and other clinical signs of periodonitis, such as probing pocket depth, attachment level, and presence of bleeding on probing at the same site. the study material comprised 16 subjects with or ... | 1990 | 2288212 |
| species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria. | oligodeoxynucleotide probes were developed for identification of the periodontal bacteria actinobacillus actinomycetemcomitans, bacteroides gingivalis, b. intermedius types i and ii, b. forsythus, eikenella corrodens, fusobacterium nucleatum, haemophilus aphrophilus, streptococcus intermedius, and wolinella recta. probes were designed by sequencing the 16s rrna for each bacterium, identifying hypervariable regions, and chemically synthesizing species-specific probes. these probes were specific w ... | 1990 | 2312676 |
| the obligate and facultatively anaerobic bacterial flora of the normal feline gingival margin. | samples from the gingival margins of 14 cats considered normal on clinical examination were cultured for facultative and obligate anaerobic bacteria. all mouths were free from any gingival marginal inflammation and tartar build-up; all cats were between 6 and 12 months of age. a mixed growth was obtained from all samples. the mean number of bacterial species per sample was 10.7 with a range of 7-16 isolates. of the 150 isolates processed, 109 (72.66%) were obligate anaerobes. of the facultativel ... | 1990 | 2353448 |
| surface characteristics of wolinella recta atcc 33238 and human clinical isolates: correlation of structure with function. | selected characteristics of the surface of wolinella recta atcc 33238 and three w. recta clinical isolates (ci) were studied as well as the adherence of these strains to human gingival fibroblasts (hgf). w. recta atcc 33238 and the ci were examined by electron microscopy, electrophoresis, isoelectric focusing, and adherence to hgf. electron microscopic examination of ci revealed the presence of a periodic paracrystalline layer external to and associated with the outer membrane. this surface laye ... | 1990 | 2387623 |
| wolinella succinogenes fumarate reductase contains a dihaem cytochrome b. | the fumarate reductase operon of wolinella succinogenes is made up of three structural genes (frd-cab). the frdc gene was located next to the promoter region and identified as the cytochrome b structural gene encoding 256 amino acid residues. the n-terminal amino acid sequences of seven fragments derived from the cytochrome b moiety of the enzyme all mapped within the frdc gene. this suggested that the enzyme contained only one species of cytochrome b. re-evaluation of earlier measurements of su ... | 1990 | 2388563 |
| antimicrobial effect of chlorhexidine in a controlled release delivery system. | chlorhexidine is widely used as a mouth rinse in the prevention and treatment of periodontal diseases and dental caries. the purpose of the present study was to evaluate the in vitro antimicrobial effect of chlorhexidine in a controlled release delivery system. the controlled release dispenser comprised a polymeric inner core matrix containing the medicament with an outer vinyl membrane controlling the drug release. the effect on the following bacteria was studied: actinobacillus actinomycetemco ... | 1990 | 2390965 |
| microbiological aspects of helicobacter pylori (campylobacter pylori). | the human gastric pathogen campylobacter pylori has recently been reclassified as helicobacter pylori, and a related spiral bacterium found in the stomach of ferrets has been designated helicobacter mustelae. the general microbiological features of helicobacter pylori are delineated here, with details of phenotypic differences between helicobacter pylori and helicobacter mustelae; comparisons are made with wolinella succinogenes and campylobacter jejuni. the helicobacter organisms possess an ext ... | 1990 | 2406141 |
| ultrastructural relationship of cell envelope layers in wolinella recta. | the cell envelope of wolinella recta was studied by electron microscopy. in thin sections the surface layer (s-layer) seemed to be in close contact to the outer membrane (om). a hexagonally arranged pattern of the s-layer was revealed by freeze-etching. unlike in other oral bacteria with an s-layer, the cleavage occurred along the s-layer by freeze-fracture, suggesting an exceptionally tight relationship between the two outermost layers. the convex surface of the s-layer was usually revealed by ... | 1989 | 2469124 |
| dna-dna hybridization incompatibility of campylobacter pylori with other campylobacter and wolinella species. | dna-dna hybridization in solution was used to characterize 23 human isolates of campylobacter pylori. the 23 isolates showed dna affinity with the type strain (nctc 11637). the relative binding ratios varied between 0.83 and 1. type strains of c. coli (nctc 11366), c. jejuni (nctc 11351), c. laridis (nctc 11352), c. sputorum subsp. sputorum (atcc 35980), wolinella recta (nctc 11489) and w. succinogenes (atcc 29543) showed relative binding ratios less than 0.01 compared to the c. pylori type stra ... | 1989 | 2499346 |
| in-vitro activity of ciprofloxacin and other agents against oral bacteria. | the susceptibility of 102 bacterial strains isolated from oral infections to ciprofloxacin, penicillin v, tetracycline and metronidazole was assessed by an agar dilution technique. penicillin v was the most effective of the antimicrobials, with only three isolates resistant, while widespread resistance to tetracycline was seen. metronidazole, as expected, was extremely effective against anaerobic isolates. ciprofloxacin exhibited intermediate activity with the majority of mics in the range of 1- ... | 1989 | 2513308 |
| purification and some characteristics of a cytochrome c-containing nitrous oxide reductase from wolinella succinogenes. | nitrous oxide reductase from wolinella succinogenes was purified very nearly to homogeneity. the enzyme was found to be dimeric, with mr = 162,000 and subunit mr = 88,000, and to contain three copper atoms and one iron atom (as cytochrome c) per subunit. the oxidized enzyme exhibited absorption bands at 410 and 528 nm, and the dithionite-reduced enzyme, at 416, 520, and 550 nm. the isoelectric point was 8.6; specific activity was at 25 degrees c and ph 7.1, 160 mumol x min-1 x mg-1; and km was 7 ... | 1989 | 2536696 |
| chemical and biological properties of lipopolysaccharide, lipid a and degraded polysaccharide from wolinella recta atcc 33238. | lipopolysaccharide (lps) was isolated and purified from wolinella recta atcc 33238 by the phenol-water procedure and rnaase treatment. the sugar components of the lps were rhamnose, mannose, glucose, heptose, 2-keto-3-deoxyoctonate (kdo) (3-deoxy-d-manno-octulosonate) and glucosamine. the degraded polysaccharide prepared from lps by mild acid hydrolysis was fractionated by sephadex g-50 gel chromatography into three fractions: (1) a high-molecular-mass fraction, eluting just behind the void volu ... | 1989 | 2600585 |
| newly delineated periodontal pathogens with special reference to selenomonas species. | many new species have been isolated from subgingival periodontal pockets, for example wolinella recta and bacteroides forsythus, reflecting better basic microbiological techniques. other species were created from existing species as a result of better characterization methods, i.e. bacteroides buccae and bacteroides oris. we can recognize different types of periodontal disease and can find differences relating to the progressive compared to inactive lesions. data illustrated in this presentation ... | 1989 | 2661440 |
| wolinella recta and spirochetes in relation to alteration of probing depth in a population without regular dental care. | the purpose of this study was to determine the occurence of spirochetes and wolinella sp. in untreated, nonselected subjects and to relate the presence of these organisms to changes in probing depth over a period of 180 days. clinical parameters were recorded and subgingival microbial samples were taken mesiobuccally on the upper first molars of 120 subjects (mean age 37 +/- 14 years) with a history of no regular dental care. the number of motile rods and spirochetes were determined in the darkf ... | 1989 | 2678458 |
| [microbial flora of periodontitis and monitoring of the effect of initial preparation by immunofluorescence microscopy]. | the effect of initial preparation in adult periodontitis was evaluated by changes in clinical parameters and immunofluorescence microscopic counts of periodontal disease associated bacteria. subgingival plaque samples were taken with sterilized paper points from 10 sites of 5 periodontally healthy persons and 44 sites of 23 adult periodontitis patients. twenty-one diseased sites were periodically examined after plaque control and scaling, root planing. the direct immunofluorescence technique was ... | 1989 | 2700361 |
| subgingival microflora, dental and periodontal conditions in patients with hereditary fructose intolerance. | hereditary fructose intolerance (hfi) is a rare autosomally recessive disease which leads to severe hypoglycemia. the fructose-free diet of these patients apparently influences dental health. half of the patients are free of caries, but there are no reports on their periodontal condition nor on the composition of their subgingival microflora. therefore 18 patient with hfi were examined for the following parameters: radiographic bone loss, caries rate, gingival bleeding, occurrence of calculus, p ... | 1989 | 2704977 |
| campylobacter-wolinella group organisms are the only oral bacteria that form arylsulfatase-active colonies on a synthetic indicator medium. | most oral bacteria tested formed colonies on a chemically defined medium with a chromogenic arylsulfatase substrate. arylsulfatase activity was, however, restricted to campylobacter-wolinella group organisms, including wolinella recta, a possible periodontopathogen. w. recta was the only arylsulfatase-active species against which consistently high levels of antibody were detected in human sera. | 1989 | 2707850 |