| localization of nors in chromosomes of mouse cell lines by a combined 33258-hoechst and ag-staining technique. | | 1979 | 84805 |
| [intratypical antigenic differentiation of poliovirus strains circulating in mali by means of cross-adsorbed sera]. | the intratypic antigenic differentiation of poliovirus strains isolated from sick or healthy children in mali in 1975-1976 was performed. the method is based on the use of cross-adsorbed sera in the neutralization tests in which the virus is titrated by the plaque procedure or by the cytopathic effect. all the examined strains of serologic types i, ii, and iii were distinguished by the antigenic structure from the vaccine strains and were classified as "wild". quantitative differences in the ant ... | 1978 | 86237 |
| quantitation of particles in the freeze-fractured nuclear membrane after renal ischemia. | changes in the number and sizes of membrane-associated particles have been quantitated in the protoplasmic (p) and exoplasmic (e) fracture faces of the outer membrane of nuclei isolated from the inner cortex following renal ischemia and reflow in the rat. no changes were observed in the inner nuclear membrane. after 20-min ischemia, the number of particles in both fracture faces decreased. with reflow, the total number of particles decreased after both 20- and 60-min ischemia. the partition coef ... | 1978 | 98907 |
| [effect of polychlorinated biphenyls in the rat: ii.--preliminary report on metabolic alterations (author's transl)]. | rats were fed with a diet containing 10 and 100 ppm (wet weight) of phenoclor dpr for 4 weeks in order to evaluate the toxicity of a french pcb mixture. the influence of pcb was studied by determining the liver weights, the hepatic and muscular proteins, glycogen and humidity content as well as hematocrite, glucose, proteins and lipids in the plasma, and the lipidic contents of the liver and the carcass. there was a significant increase in both liver to body weight ratios and liver lipid and pro ... | 1978 | 105425 |
| properties of the bacillus subtilis spore coat. | about 70% of the protein in isolated bacillus subtilis spore coats was solubilized by treatment with a combination of reducing and denaturing agents at alkaline ph. the residue, consisting primarily of protein, was insoluble in a variety of reagents. the soluble proteins were resolved into at least seven bands by sodium dodecyl sulfate gel electrophoresis. about one-half of the total was four proteins of 8,000 to 12,000 daltons. these were relatively tyrosine rich, and one was a glycoprotein. th ... | 1979 | 108242 |
| the possible role of tightly bound adenine nucleotides in oxidative and photosynthetic phosphorylation. | the tightly bound nucleotides of the beff-heart mitochondrial atpase are released during cold inactivation followed by ammonium sulfate precipitation. during incubation at 0 degrees c the sedimentation coefficient (s20w) of the atpase first declines from 12.1s to 9s. prolonged incubation or precipitation with ammonium sulfate leads to dissociation of the 9s component into subunits with s20w of 3.5s. the 9s component still bears bound nucleotides which exchange more extensively and rapidly with a ... | 1975 | 127089 |
| altered activity of hepatic mixed-function mono-oxygenase enzymes in streptozotocin-induced diabetic rats. | 1. in streptozotocin-induced diabetic male rats, hepatic microsomal aminopyrine n-demethylase activity was depressed, whereas aniline hydroxylase activity and cytochrome p-450 content were increased over control values. 2. in diabetic female rats, hepatic microsomal aminopyrine n-demethylase activity, aniline hydroxylase activity, biphenyl 4-hydroxylase activity, and cytochrome p-450 content were increased over control values. 3. insulin treatment of diabetic male and female rats antagonized all ... | 1978 | 152519 |
| interactions of folch-lees proteolipid apoprotein with planar lipid bilayers. | water-soluble folch-lees proteolipid apoprotein from bovine cns white matter induces a voltage-dependent conductance in black lipid membranes. na+ is required for the induced conductance change but the established conductance has very low ionic selectivity. the induced conductance fluctuates with a minimum amplitude of 10(-11)--10(-10) mho. the magnitude of the conductivity change is dependent on protein concentration and on the composition of lipid bilayers. at a fixed voltage the induced condu ... | 1979 | 522128 |
| antiinflammatory beta-arylamidoacrylic acids. | a series of 34 beta-arylamidoacrylic acids was prepared and examined for antiinflammatory activity. these compounds are vinylogous carbamic acids, and several displayed activity equal to phenylbutazone in the rat pleural effusion model. highest activity was associated with structures bearing halogen and cyano substituents. amides were inactive. | 1975 | 1151961 |
| gene cloning of porphyromonas gingivalis specific antigens recognized by serum of adult periodontitis patient. | 1. porphyromonas gingivalis is believed an important pathogen of adult periodontitis. a gene library of p. gingivalis 381 was constructed in lambda phage vector l47.1. the library was probed with serum obtained from patients of severe adult periodontitis. two clones, lambda mdbg101 and lambda mdbg103 which were expressed, 200 and 160 kda respectively, were selected and further studied. 2. the expressed antigens in these two clones were also reacted with rabbit antiserum against whole cells, caps ... | 1992 | 1319357 |
| detubularization-induced contractile response change of the ileum following ileocystoplasty. | we reported previously that following ileocystoplasty the structure and pharmacologic response of the implanted ileum changes towards that of the bladder. specifically, the relaxation response to alpha adrenergic (methoxamine) and purinergic (atp) stimulation reverses to a contractile response one month after the ileal segment is surgically made part of the urinary bladder. the present study was designed to investigate possible signals for this change and also to determine whether bladder respon ... | 1992 | 1351953 |
| emended descriptions of prevotella denticola, prevotella loescheii, prevotella veroralis, and prevotella melaninogenica. | during studies of human periodontal disease, a number of bacterial strains were encountered that, on the basis of results of standard biochemical tests, appeared to be prevotella buccalis, prevotella denticola, prevotella melaninogenica, or prevotella loescheii. however, use of the standard biochemical tests, cellular fatty acid analyses, and the polyacrylamide gel electrophoresis patterns of soluble proteins resulted in conflicting identifications of these strains. the results of tests for cell ... | 1992 | 1390106 |
| suppressive effect of soluble factor(s) derived from prevotella loescheii atcc 15930 on proliferation of human lymphocytes. | soluble sonic extracts of prevotella loescheii caused a dose-dependent inhibition of human peripheral blood lymphocyte proliferation by mitogen and of the proliferation of a leukemic cell line, ball-1, when assessed by dna synthesis (3h-thymidine incorporation). rna (3h-uridine incorporation) and protein (3h-leucine incorporation) synthesis were similarly altered after exposure to the extract. there was no effect on cell viability as measured by either trypan blue exclusion or extracellular rele ... | 1992 | 1408357 |
| the gene encoding a prevotella loescheii lectin-like adhesin contains an interrupted sequence which causes a frameshift. | we cloned and sequenced the prevotella loescheii gene plaa, which encodes a lectin-like adhesin that mediates the coaggregation of p. loescheii 1295 with streptococcus oralis 34. a probe derived from the n-terminal amino acid sequence of the purified adhesin was used to identify the plaa gene from a p. loescheii genomic library constructed in lambda gem-11. sequence analysis of plaa indicates that the initial translation product contains a 22-amino-acid leader. the reading frame of the plaa gene ... | 1992 | 1429455 |
| relationship between oral gram-negative anaerobic bacteria in saliva of the mother and the colonization of her edentulous infant. | various gram-negative anaerobic bacterial species can be detected in the oral flora of edentulous infants. however, knowledge of the initial infection source is still scarce. thirty caucasian mothers (mean age 30 years) and their edentulous infants (mean age 3 months) were examined for the possible similarity of the oral gram-negative anaerobic flora. paraffin-stimulated saliva was collected from the mothers. a pooled swab sample from mucosal surfaces and unstimulated saliva were collected from ... | 1992 | 1494450 |
| the early colonization of gram-negative anaerobic bacteria in edentulous infants. | the occurrence of oral gram-negative anaerobes was examined in 30 edentulous infants (mean age 3 months, range 1-7 months). one pooled swab sample from mucosal surfaces (cheeks, palate, tongue) and one saliva sample was taken from each infant. the samples were cultured aerobically and anaerobically using non-selective and selective media. prevotella (bacteroides) melaninogenica was the most frequently isolated anaerobe, found in 70% of the infants. the other common anaerobes were fusobacterium n ... | 1992 | 1528621 |
| degradation of native human hemoglobin following hemolysis by prevotella loescheii. | prevotella loescheii pk1295 can grow on native hemoglobin as a source of heme. supernatants of p. loescheii cultures hemolysed human erythrocytes and degraded native hemoglobin. these combined activities may provide heme (or iron) for the growth of p. loescheii and other dental plaque bacteria. | 1992 | 1548099 |
| rapid presumptive identification of black-pigmented gram-negative anaerobic bacteria by using 4-methylumbelliferone derivatives. | a rapid method for presumptive identification of black-pigmented gram-negative anaerobic rods was developed. using filter paper spot tests for indole production, sialidase, alpha-glucosidase, beta-glucosidase, alpha-fucosidase, and trypsinlike enzyme activities, 100% of porphyromonas gingivalis, prevotella intermedia, and bacteroides levii and 89% of prevotella corporis isolates were correctly identified to the species level. porphyromonas asaccharolytica and porphyromonas endodontalis could not ... | 1991 | 1774320 |
| purification and properties of neuraminidase from the culture medium of bacteroides loescheii. | neuraminidase was purified from the culture medium of bacteroides loescheii atcc 15930 by ultrafiltration followed by a deae-sephacel anion exchange chromatography, fast-protein liquid chromatography using mono p column and high-performance liquid chromatography using a shim-pack diol-300 column. the molecular weight of the purified enzyme was measured to be approximately 87 kda and the optimal ph was at 4.8. although the enzyme was able to hydrolyze the substrates with alpha,2-3, alpha,2-6, and ... | 1991 | 1820573 |
| major salivary gland lymphoepithelial lesions and the acquired immunodeficiency syndrome. | presently, there is no consensus regarding the most appropriate diagnostic and therapeutic approach to patients with human immunodeficiency virus (hiv)-associated lymphoepithelial lesions of the major salivary glands. a retrospective review of 60 consecutive patients with lymphoepithelial lesions is presented. thirty-eight cases were associated with hiv infection. lesions associated with hiv infection were usually bilateral, multiple, cystic, and associated with lymphadenopathy. in contrast, in ... | 1991 | 1951883 |
| purification and characterization of a bacteroides loeschei adhesin that interacts with procaryotic and eucaryotic cells. | the adhesin of bacteroides loeschei pk1295 that mediates coaggregation with streptococcus sanguis 34 and hemagglutination of erythrocytes was purified to electrophoretic homogeneity. the lectinlike protein has an estimated native mr of 450,000 and consists of six subunits of identical molecular weight (mr 75,000). the purified adhesin appears to be a basic protein with a pi between 7.4 and 8.0. amino acid and n-terminal sequence analyses were carried out with the purified protein. these indicate ... | 1990 | 1970562 |
| the formation of hydrogen sulfide and methyl mercaptan by oral bacteria. | the capacity to form volatile sulfur compounds was tested in bacteria isolated from subgingival microbiotas and in a representative number of reference strains. a majority of the 75 tested oral bacterial species and 7 unnamed bacterial taxa formed significant amounts of hydrogen sulfide from l-cysteine. the most active bacteria were found in the genera peptostreptococcus, eubacterium, selenomonas, centipeda, bacteroides and fusobacterium. methyl mercaptan from l-methionine was formed by some mem ... | 1990 | 2082242 |
| the incidence of periodontopathic microorganisms in young children. | thirty-six children in 4 cohorts of 0-1 week, 1-6 months, 1-2 years and 2-2 1/2 years of age were examined for the presence of black-pigmented bacteroides species and some other bacteria suspected of being involved in the subsequent development of periodontal destruction. none of the sought-after bacteria were detected in the first week of life. bacteroides intermedius and bacteroides melaninogenicus were detected as early as 1 month after birth. both of these bacteria were detected in 16-37% of ... | 1990 | 2087345 |
| [purification and some properties of neuraminidase from the culture medium of bacteroides loescheii atcc 15930]. | the purpose of this study is to investigate the mechanism on enhanced hemagglutination caused by neuraminidase from bacteroides in an aspect of zeta-potential levels on erythrocytes and inhibitors on hemagglutination activity. the neuraminidase from the culture medium of bacteroides loescheii atcc 15930 was purified by ultrafiltration followed by deae-sephacel anion exchange chromatography, fast protein, polypeptide, polynucleotide, liquid chromatography using mono p column and finally high perf ... | 1989 | 2488923 |
| fimbria-associated adhesin of bacteroides loeschei that recognizes receptors on procaryotic and eucaryotic cells. | inhibition studies with a set of adhesin-specific monoclonal antibodies and various sugars revealed that a fimbria-associated adhesin of bacteroides loeschei recognizes receptors on both procaryotic and eucaryotic cells. these interactions permit this bacterium to attach to both types of cells, producing coaggregates in the presence of strains of streptococcus sanguis and hemagglutination in the presence of neuraminidase-treated human erythrocytes. | 1989 | 2569449 |
| bacteroides loeschei pk1295 cells express two distinct adhesins simultaneously. | bacteroides loeschei synthesizes two distinct adhesins that mediate its coaggregation with streptococcus sanguis 34 and actinomyces israeli pk14. streptococcal adhesin-specific and actinomyces adhesin-specific monoclonal antibodies were used to prepare antibody-coated 5- or 10-nm gold particles. these were used in immunoelectron microscopic studies to establish that essentially all bacteroides cells in a population express both adhesins. in general, the two sizes of gold particles representing e ... | 1989 | 2572563 |
| prevalence of black-pigmented bacteroides species in root canal infections. | the prevalence of black-pigmented bacteroides species in the root canals of 72 teeth with apical periodontitis was evaluated. twenty-two of the canals contained one or more species of black-pigmented bacteroides. bacteroides intermedius (14 strains) and bacteroides endodontalis (5 strains) were most common. of the species bacteroides gingivalis, bacteroides loeschei, and bacteroides denticola, 2, 3, and 1 strains, respectively, were isolated. the median number of bacterial cells recovered from t ... | 1989 | 2607261 |
| further studies on the degradation of immunoglobulins by black-pigmented bacteroides. | the ability of several species of black-pigmented bacteroides to degrade immunoglobulins a and g was confirmed in this study. the cleavage products from igg strongly stimulated the growth of bacteria degrading igg. growth of bacteroides gingivalis on limiting media supplemented with igg paralleled growth on complete medium. the degradation of igg and iga by black-pigmented bacteroides appeared to occur in 2 stages. the molecules were broken into large fragments which were subsequently degraded i ... | 1989 | 2628862 |
| immunomodulatory effects of bacteroides products on in vitro human lymphocyte functions. | bacteroides spp. have been implicated in the pathogenesis of several diseases, including periodontal diseases. in this study sonic extracts of 6 bacteroides spp. were examined for their abilities to alter human lymphocyte function. we found that soluble extracts from bacteroides intermedius, bacteroides endodontalis, bacteroides asaccharolyticus, bacteroides melaninogenicus, and to a lesser degree bacteroides loescheii, caused dose-dependent inhibition of human lymphocyte responsiveness to both ... | 1989 | 2628864 |
| energetics and mechanics of contracting white myotomal fibres from dogfish, scyliorhinus canicula. | | 1989 | 2798483 |
| energetics and mechanics of contracting white myotomal fibres from dogfish, scyliorhinus canicula. | | 1989 | 2798483 |
| low-grade lymphomas in young homosexual men without lav/htlv iii infection. | | 1986 | 2877052 |
| the challenge of malaria vaccine: trials and tribulations. | | 1987 | 2880172 |
| fimbria-associated proteins of bacteroides loescheii pk1295 mediate intergeneric coaggregations. | bacteroides loescheii pk1295 serves as a coaggregation bridge between streptococcus sanguis 34 and actinomyces israelii pk14, two gram-positive oral bacteria that are otherwise unable to coaggregate. whereas coaggregation with s. sanguis 34 is inhibited by lactose, no simple sugar was found that inhibited coaggregation with a. israelii pk14. coaggregation-defective (cog-) mutants of b. loescheii pk1295 were isolated for the purpose of identifying the surface components responsible for the intera ... | 1987 | 2887546 |
| characterization of monoclonal antibodies to fimbria-associated adhesins of bacteroides loescheii pk1295. | bacteroides loescheii pk1295 fimbriae, which mediate the lactose-sensitive coaggregation with streptococcus sanguis 34 and the lactose-insensitive coaggregation with actinomyces israelii pk14, were injected into mice to raise adhesin-specific monoclonal antibodies (mabs). supernatants of hybridomas were screened for the capacity to inhibit coaggregation and agglutinate intact bacteria. of the 10 mabs that were isolated, 4 were specific and potent inhibitors of the coaggregation between b. loesch ... | 1988 | 2891617 |
| localization and enumeration of fimbria-associated adhesins of bacteroides loescheii. | monoclonal antibodies that specifically inhibit coaggregation between bacteroides loescheii pk1295 and its two gram-positive partners streptococcus sanguis 34 and actinomyces israelii pk14 were used to enumerate and localize two distinct types of fimbria-associated adhesins on the surface of b. loescheii. binding studies with radiolabeled monoclonal antibodies indicated that a maximum (bmax calculated from scatchard plots) of approximately 400 adhesin molecules specific for s. sanguis and 310 ad ... | 1988 | 2893786 |
| a computer-aided analysis of the effect of peripheral nerve transection on tmpase activity of substantia gelatinosa rolandi. | | 1986 | 3020818 |
| regional localization of cd18, the beta-subunit of the cell surface adhesion molecule lfa-1, on human chromosome 21 by in situ hybridization. | the gene coding for the beta-subunit of the cell surface adhesion glycoprotein lfa-1 has been localized to the tip of the long arm of chromosome 21 at 21q22.1-qter. | 1988 | 3073708 |
| fatty acids and sugars in lipoplysaccharides from bacteroides intermedius. bacteroides gingivalis and bacteroides loescheii. | | 1988 | 3268745 |
| the role of black-pigmented bacteroides in human oral infections. | today, 10 black-pigmented bacteroides (bpb) species are recognized. the majority of these species can be isolated from the oral cavity. bpb species are involved in anaerobic infections of oral and non-oral sites. in the oral cavity, bpb species are associated with gingivitis, periodontitis, endodontal infections and odontogenic abscesses. cultural studies suggest a specific role of the various bpb species in the different types of infection. bacteroides gingivalis is closely correlated with dest ... | 1988 | 3280611 |
| intergeneric rosettes: sequestered surface recognition among human periodontal bacteria. | the human oral bacteria, streptococcus sanguis and bacteroides loescheii, when mixed in equal numbers in vitro, formed large settling coaggregates. as the relative number of each cell type was changed, coaggregates became smaller until at cell-type ratios of 10 to 1, rosettes formed. rosettes consisting of a streptococcal cell in the center surrounded by bacteroides cells exhibited surface recognition properties of only the bacteroides, which coaggregated with many other cell types such as actin ... | 1988 | 3377498 |
| evidence of estrogen receptors in normal human osteoblast-like cells. | in seven strains of cultured normal human osteoblast-like cells, a mean of 1615 molecules of tritium-labeled 17 beta-estradiol per cell nucleus could be bound to specific nuclear sites. the nuclear binding of the labeled steroid was temperature-dependent, steroid-specific, saturable, and cell type-specific. these are characteristics of biologically active estrogen receptors. pretreatment with 10 nanomolar estradiol in vitro increased the specific nuclear binding of progesterone in four of six ce ... | 1988 | 3388021 |
| effect of a protease derived from the oral bacterium bacteroides loescheii on the inhibition of calcium-phosphate precipitation by human parotid saliva. | a protease from the culture fluid of this microorganism was purified and characterized using deae-sephadex and chromatofocusing and classified as a thiol protease with a mol. wt estimated as 27,500. it abolished the inhibitory activity of parotid saliva on the precipitation of calcium phosphate from supersaturated solutions. such proteases may contribute to dental calculus formation. | 1986 | 3541865 |
| a computerized system for measuring detection sensitivity over the visual field. | a device is described for comprehensively measuring the capability of human operators to detect and react to visual signals occurring at a variety of locations throughout the functional visual field. the system is completely automated and computerized, and provides both documentation files and descriptive graphics of the operator's performance immediately upon completion of testing. sensitivity of detection can be measured for red, yellow, and green stimulus colors in a testing configuration whi ... | 1987 | 3632544 |
| influence of inoculation sites and tumor cell culture techniques on the phenotype of a mixed cartilage- and bone-producing mouse mammary tumor. | we have previously reported the characterization of an intraperitoneally (ip) transplantable bone-forming mxt tumor. however, the question was unresolved as whether the bone-forming cells originated from either the host animal or from the neoplasm itself. the present work attempts to answer this question by studying the influences of inoculation sites (subcutaneously, sc; intraperitoneally, ip; in the brain, ib; intracranially, icr) on both the cartilage- and bone-forming tumor phenotypes. furth ... | 1986 | 3813489 |
| cloning of the e. coli o6-methylguanine and methylphosphotriester methyltransferase gene using a functional dna repair assay. | alkylating agents react with various nitrogen and oxygen atoms in dna and many of the products are substrates for repair processes. oxygen atom derivatives such as o6-methylguanine (o6-meg) o4-methylthymine and methylphosphotriesters (mp) have been shown to undergo repair by methyl group removal. the proteins involved in the latter reaction can be considered to be methyltransferases (mt) because their action results in the transfer of the methyl group to a cysteine residue within a polypeptide. ... | 1985 | 3889845 |
| cloning of the e. coli o6-methylguanine and methylphosphotriester methyltransferase gene using a functional dna repair assay. | alkylating agents react with various nitrogen and oxygen atoms in dna and many of the products are substrates for repair processes. oxygen atom derivatives such as o6-methylguanine (o6-meg) o4-methylthymine and methylphosphotriesters (mp) have been shown to undergo repair by methyl group removal. the proteins involved in the latter reaction can be considered to be methyltransferases (mt) because their action results in the transfer of the methyl group to a cysteine residue within a polypeptide. ... | 1985 | 3889845 |
| antimycotic 2-alkyldithio, 2-aralkyldithio, 2-aryldithiobenzamides. | some 2-alkyldithio, 2-aralkyldithio, 2-aryldithio benzoic acids, their methyl esters and n-monosubstituted amides were prepared and tested in vitro against candida albicans and trichophyton mentagrophytes. some n-monosubstituted amides displayed activities similar to those of clotrimazole and pyrrolnitrin. against candida albicans, n-monosubstituted amides exhibited a generally higher activity than the corresponding n-monosubstituted amides of 2,2'-dicarboxydiphenyldisulfide. | 1985 | 3910452 |
| [effects of nitronaphthofurans on the transfer of a plasmid]. | | 1985 | 3915195 |
| striatal tyrosine hydroxylases with high and low affinity for tyrosine: implications for the multiple-pool concept of catecholamines. | | 1973 | 4148015 |
| suppressor cells in the rabbit appendix. | | 1974 | 4547314 |
| tobacco smoke: a noxious air pollutant. a review (and comment). ii of ii. tobacco smoke: general characteristics and composition. | | 1971 | 4930344 |
| taxonomy of anaerobes: present state of the art. | results of nucleic acid studies, which indicate relationships among strains and species more clearly than do usual phenotypic tests, have led to new bacteriologic nomenclature. some major changes in bacteroides include the recognition of three species (bacteroides melaninogenicus, bacteroides denticola, and bacteroides loescheii) formerly grouped in b. melaninogenicus subspecies melaninogenicus; two species (bacteroides intermedius with two closely related homology groups and bacteroides corpori ... | 1984 | 6201990 |
| bacteriological study of periodontal lesions in two sisters with juvenile periodontitis and their mother. | a total of five bacteriological samples from the periodontal pockets of two sisters with localized juvenile periodontitis and their mother with advanced periodontitis was studied. gram-negative anaerobic rods were predominant in the samples. bacteroides intermedius and bacteroides loescheii were the most predominant species. the antigenicity and bacteriocinogenicity of these isolates were quite similar. serum immunoglobulin g antibody levels of the subjects to gram-negative periodontopathic bact ... | 1984 | 6429040 |
| immunoglobulin g response to subgingival gram-negative bacteria in human subjects. | serum and gingival crevicular fluid from normal healthy adults and patients with periodontitis were screened for immunoglobulin g antibodies to antigens from bacteroides gingivalis 381, bacteroides intermedius 24, bacteroides loescheii atcc 15930, fusobacterium nucleatum atcc 25586, eikenella corrodens 1073, actinobacillus actinomycetemcomitans atcc 29522, and capnocytophaga sp. strain m-12. immunoglobulin g antibody titers to the antigens were measured by an enzyme-linked immunosorbent assay. t ... | 1984 | 6735473 |
| effect of proteolytic enzymes on the lysis and growth of oral bacteria. | previous studies have shown increased levels of proteolytic enzymes in affected periodontal sites. the aim of the present investigation was to evaluate the effect of proteolytic environments on the lysis and growth of selected oral bacteria associated with either healthy or diseased periodontal sites. the effect of trypsin, chymotrypsin and proteinase k on cell lysis was determined following incubation with bacteria, whereas the effect of the same proteolytic enzymes on bacterial growth was test ... | 1994 | 7478762 |
| the tet(q) gene in bacteria isolated from patients with refractory periodontal disease. | twenty-two tetracycline-resistant (tetr) anaerobic and facultative anaerobic bacteria isolated from periodontal pockets of 12 patients with refractory periodontitis were examined for the presence of the tet q determinant by dna-dna hybridization. dot blots of bacterial dna were tested with an intragenic digoxigenin-labelled tet(q) probe consisting of a 1.45 kb ecori/pvuii fragment from plasmid pnfd13-2. southern blots of chromosomal dna digested with the restriction enzyme ecori were also examin ... | 1994 | 7478767 |
| effect of periodontal treatments on serum igg antibody titers against periodontopathic bacteria. | serum igg antibody titers to 7 periodontopathic bacteria in periodontitis patients were measured at the 1st visit and after various periodontal treatments with clinically successful improvement, in order to evaluate what kind of factors are associated with changes of serum antibody titers. 20 patients (10 male and 10 female from 23 to 61 years old) with adult, rapidly progressive periodontitis were enrolled in this study. all patients received initial preparation and most of them also underwent ... | 1995 | 7560233 |
| volatile fatty acids, metabolic by-products of periodontopathic bacteria, inhibit lymphocyte proliferation and cytokine production. | short-chain fatty acids are a major by-product of anaerobic metabolism and can be detected in gingival fluid from periodontal pockets. since most t cells are present subjacent to the pocket epithelium in conjunction with the plasma cells, it is important to know how these t cells are affected by short-chain fatty acids produced by subgingival plaque. the purpose of this study is to examine the effects of extracellular metabolites from periodontopathic bacteria on the proliferation and cytokine p ... | 1995 | 7560387 |
| adhesin degradation: a possible function for a prevotella loescheii protease? | prevotella loescheii pk1295 produces at least 3 proteases that are separable by isoelectric focusing. one of these proteases, an enzyme with an isoelectric point at 8.5 and an m(r) of 36,000, hydrolyzes the fimbria-associated adhesin on p. loescheii responsible for coaggregation with streptococcus oralis 34, as well as gelatin, casein and fibrin. the action of this protease may contribute to the detachment of p. loescheii from its streptococcal coaggregation partner and provide a mechanism for b ... | 1993 | 7903444 |
| the oral gram-negative anaerobic microflora in young children: longitudinal changes from edentulous to dentate mouth. | eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the composition of oral gram-negative anaerobic microflora was investigated in 21 young children (mean age 32 months) with primary dentition. the bacterial findings of samples were compared with those of the same children collected at their edentulous infant period (mean age 3 months). during the primary period, 2 samples were collected from each child: a sample with d ... | 1994 | 7936718 |
| the most frequently isolated gram-negative anaerobes in saliva and subgingival samples taken from young women. | stimulated saliva and a pooled subgingival sample from the first molars were collected from 30 caucasian women (mean age 30 years) for the presence of gram-negative anaerobic bacteria. the samples were inoculated on nonselective and selective media. the gram-negative anaerobic species occurring in the oral cavity of more than 50% of the subjects were selected for further analysis. fusobacterium nucleatum and prevotella melaninogenica were not recovered from subgingival sites without a simultaneo ... | 1994 | 8008430 |
| expression of the prevotella loescheii adhesin gene (plaa) is mediated by a programmed frameshifting hop. | the 2.4-kb plaa gene, which encodes a prevotella loescheii galactoside-specific adhesin, contains a programmed frameshifting hop. the frameshift region consists of two uaa termination codons, two repeats of four identical bases between the terminators, and a stem-loop structure that has the potential to form a pseudoknot located downstream from the second uaa. the stem-loop and pseudoknot are features found in a number of retroviruses where frameshifting is a more common occurrence. the terminat ... | 1994 | 8144461 |
| coaggregation: specific adherence among human oral plaque bacteria. | nearly all human oral bacteria exhibit coaggregation, cell-to-cell recognition of genetically distinct cell types. clumps or coaggregates composed of the two kinds of cells are formed immediately upon mixing two partner cell types. members of all 18 genera tested exhibit lactose-reversible coaggregation. many of these interactions appear to be mediated by a lectin on one cell type that interacts with a complementary carbohydrate receptor on the other cell type. a lactose-sensitive adhesin has be ... | 1993 | 8462782 |
| cell envelope and cell wall immunization of macaca fascicularis: effect on the progression of ligature-induced periodontitis. | the nonhuman primate, macaca fascicularis, was used to study the role of immunization with selected members of the periodontopathic microbiota in the longitudinal progression of ligature-induced periodontitis. animals were immunized with cell envelope antigens prepared from porphyromonas gingivalis and prevotella intermedia, and a mixture prepared from fusobacterium nucleatum, campylobacter rectus, and actinomyces viscosus. serum immunoglobulin g (igg), igm and iga isotype antibodies increased s ... | 1995 | 8602339 |
| an investigation into the use of sds-page of cell surface extracts and proteolytic activity to differentiate prevotella nigrescens and prevotella intermedia. | by comparison of the cell surface proteins derived from the outer membrane and fibrils from 14 prevotella intermedia and 19 prevotella nigrescens strains using sds and analysed by sds-page, it was possible to distinguish the two species. a polypeptide of approx. 21 kda distinguished p. intermedia strains, whereas two polypeptides of approx. 18 and 22 kda could be used to identify p. nigrescens strains. four other human oral black pigmented bacterial species (porphyromonas gingivalis, prevotella ... | 1996 | 8869494 |
| [a case of severe odontogenic infection with allergic skin reaction for beta-lactam antibiotics]. | a 54-aged woman consulted with right buccal phlegmon caused by lower apical periodontitis. the primary chemotherapy using flomoxef induced allergic skin eruption. a decision was made for using clindamycin, levofloxacin and fosfomycin as chemotherapeutic agents. cultured organisms from the abscess revealed the mixed infection with streptococcus sanguis, veillonella sp., prevotella loescheii, wolinella spp. on 12th hospital day, her serum crp turned to negative. this case carried numerous implicat ... | 1997 | 9209128 |
| inhibition of prevotella and capnocytophaga immunoglobulin a1 proteases by human serum. | oral prevotella and capnocytophaga species, regularly isolated from periodontal pockets and associated with extraoral infections, secret specific immunoglobulin a1 (iga1) proteases cleaving human iga1 in the hinge region into intact fab and fc fragments. to investigate whether these enzymes are subject to inhibition in vivo in humans, we tested 34 sera from periodontally diseased and healthy individuals in an enzyme-linked immunosorbent assay for the presence and titers of inhibition of seven pr ... | 1997 | 9220164 |
| beta-lactamase producing bacteria in adult periodontitis. | in 23 untreated adult periodontitis patients, the occurrence of beta-lactamase producing periodontal bacteria was determined. in addition to non-selective isolation media, selective isolation and growth of beta-lactamase positive subgingival bacterial species was carried out on blood agar plates supplemented with amoxicillin and plates with amoxicillin+clavulanic acid. porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, peptostreptococcus micros, fusobacterium ... | 1997 | 9266340 |
| beta-lactamase production by oral pigmented prevotella species isolated from young children. | the frequency of beta-lactamase production by oral pigmented prevotella species isolated from 23 healthy young children and the minimal inhibitory concentrations (mics) for 186 available beta-lactamase-positive isolates were examined by using the chromogenic cephalosporin disk test (ab biodisk, solna, sweden) and the etest (ab biodisk) and/or the agar dilution method of the national committee for clinical laboratory standards (villanova, pa, usa), respectively. beta-lactamase-positive prevotella ... | 1997 | 9310704 |
| mechanisms of lymphocyte adhesion to human vascular endothelial cells in culture. t lymphocyte adhesion to endothelial cells through endothelial hla-dr antigens induced by gamma interferon. | the effects of interferons (ifns) on lymphocyte adhesion to cultured human vascular endothelial cells (ec) were investigated using an in vitro assay. endothelial cells obtained from umbilical vein were first cultured at a low density with a conditioned medium (cm) from 12-o-tetra decanoylphorbol 13-acetate-concanavalin a (tpa-con a) stimulated human peripheral blood lymphocytes (pbl), or with recombinant (r) gamma interferon (ifn-gamma) or r alpha interferon (ifn-alpha), and then were incubated ... | 1986 | 3084562 |
| use of the rapid-ana system to screen for enzyme activities that differ among species of bile-inhibited bacteroides. | the rapid-ana system (innovative diagnostics systems, inc., atlanta, ga.) was used to test 102 strains of 14 species of phenotypically similar bile-inhibited bacteroides from humans. bacteroides oris, bacteroides veroralis, bacteroides buccalis, bacteroides melaninogenicus, bacteroides loescheii, and bacteroides denticola had very similar enzyme activity profiles. clear differentiation of these six species by the rapid-ana system was not possible, but tests for arginine aminopeptidase and beta-g ... | 1986 | 2871042 |
| fermentation product analysis in the identification of black-pigmented bacteria from periodontitis. | a quantitative procedure is described for the analysis of fermentation products of eight representative black-pigmented gram-negative anaerobic bacterial strains (porphyromonas gingivalis 381, porphyromonas gingivalis atcc 33277, prevotella intermedia atcc 25611, prevotella nigrescens atcc 33563, prevotella melaninogenica atcc 25845, prevotella denticola atcc 33185, and prevotella loescheii atcc 15930) from oral sites in humans, using gas-liquid chromatography. this procedure for the identificat ... | 1997 | 9345789 |
| volatile fatty acid, metabolic by-product of periodontopathic bacteria, induces apoptosis in wehi 231 and raji b lymphoma cells and splenic b cells. | the ability of butyric acid, an extracellular metabolite from periodontopathic bacteria, to induce apoptosis in murine wehi 231 cells, splenic b cells, and human raji cells was examined. the culture filtrate of porphyromonas gingivalis, prevotella loescheii, and fusobacterium nucleatum, which contains high a percentage of butyric acid, induced dna fragmentation in wehi 231 cells. volatile fatty acid, especially butyric acid, significantly suppressed b-cell viability in a concentration-dependent ... | 1998 | 9596720 |
| serum antibodies reacting with subgingival species in refractory periodontitis subjects. | the purpose of this investigation was to compare the levels of serum igg antibody to 85 subgingival species in 32 refractory periodontitis, 56 successfully treated, and 33 periodontally healthy subjects. refractory subjects showed mean full mouth attachment loss and/or >3 sites showing attachment loss >2.5 mm within 1 year after 2 treatment modalities, scaling and root planing and surgery plus systemically administered tetracycline. successfully-treated subjects showed mean attachment level gain ... | 1998 | 9696261 |
| the translational hop junction and the 5' transcriptional start site for the prevotella loescheii adhesin encoded by plaa. | the prevotella loescheii adhesin gene, plaa, contains a coding gap between a small open reading frame (orf-1) and a large open reading frame (orf-2). translation of the plaa mrna requires bypassing this 29-nt coding gap on the plaa transcript. we have determined the n-terminal peptide sequence of the so34 adhesin beyond the gap sequence. this sequence shows that the peptide junction between orf-1 and orf-2 is continuous in the adhesin and supports the conclusion that synthesis of the so34 adhesi ... | 1999 | 9841777 |
| a hemagglutinating variant of prevotella melaninogenica isolated from the oral cavity. | strains resembling prevotella melaninogenica were isolated from healthy subjects and patients with periodontal disease and were identified using: a 5-test phenotypic screen; commercial identification kits; and a 16s rrna-based polymerase chain reaction (pcr) method. eleven clinical isolates closely resembling p. melaninogenica, and all from patients with periodontitis, were able to agglutinate erythrocytes. in the electron microscope, hemagglutinating isolates showed fimbria-like structures, tha ... | 1998 | 9872112 |
| detection of dextranase-producing gram-negative oral bacteria. | thirty-one strains of 23 gram-negative oral bacterial species were examined for dextran-degrading activity on agar plates containing blue dextran. one strain each of capnocytophaga ochracea, capnocytophaga sputigena, prevotella loescheii, prevotella melaninogenica and prevotella oralis had detectable dextranase activity. the culture supernatants of p. melaninogenica and p. oralis cells contained dextranases of multiple sizes, but those of the other three species had a single size of enzyme. a 56 ... | 1998 | 9872116 |
| the importance of fimbriae in the virulence and ecology of some oral bacteria. | cumulative evidence indicates that bacterial adherence to mucosal and tooth surfaces as well as bacterial coaggregation are essential steps for colonization of various oral bacterial species. bacterial fimbriae have been shown to play an important role in the interaction between bacteria and host cells or among bacterial cells. the properties of fimbriae from selected species of oral bacteria are discussed in terms of virulence traits and ecological significance. among others, porphyromonas ging ... | 1998 | 10093527 |
| mutant analysis of prevotella sp. plaa-lacz fusion protein expression in escherichia coli: support for an essential role of the stem-loop. | this study investigated the involvement of rna folding in the synthesis of a fusion protein with beta-galactosidase activity. the coding gap region of the prevotella loescheii adhesin gene plaa was fused in-frame with the escherichia coli lacz gene on plasmid psk105. n-terminal sequencing of the expressed plaa-lacz protein indicated that it resulted from translational initiation at a fortuitous ribosomal-binding site within the plaa sequence at nt 570. specific mutations were introduced in the s ... | 1999 | 10380648 |
| profile of subgingival microbiota in children with primary dentition. | eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). the samples were cultured for bacterial isolat ... | 2000 | 10791707 |
| cloning, characterization, and possible origin of the prevotella loescheii dnak homolog. | heat shock proteins play an important role in bacterial survival and response to environmental stress. we cloned the prevotella loescheii hsp70 homolog (dnak) and characterized the coding sequence, regulatory regions, and evolutionary relationships to other bacteria. predicted proteins encoded by the p. loescheii dnak homolog (open reading frame orf-1) and two downstream coding regions, orf-2 and orf-3, are highly homologous to the proteins encoded by orf-4 (dnak), orf-5, and orf-6 from the dnak ... | 2001 | 11136127 |
| profile of subgingival microbiota in children with mixed dentition. | a diversity of microbial species has been detected in children's oral flora at an early age. to investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. the samples were cultured for bacterial isolation anaerobica ... | 2000 | 11155173 |
| subdural empyema caused by prevotella loescheii with reduced susceptibility to metronidazole. | | 2001 | 11222578 |
| isolation and characterisation of dipeptidyl peptidase iv from prevotella loescheii atcc 15930. | a proline-specific dipeptidyl aminopeptidase, dipeptidyl peptidase iv (ec 3.4.14.5), was purified from a cell sonicate soluble fraction of prevotella loescheii atcc 15930 by sequential column chromatography. the molecular mass of the native enzyme was estimated as 160 kda by high-pressure liquid gel filtration column chromatography and unheated sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page). the subunit molecular mass was 80 kda when the enzyme was heated to 100 degrees c ... | 2001 | 11389867 |
| predominant cultivable subgingival microbiota of healthy and hiv-infected ethnic chinese. | although clinically distinctive types of periodontal disease are known to be associated with hiv infection, the pathogenesis remains unclear. in the present study, the subgingival microflora of 21 hiv-infected and 11 hiv-free ethnic chinese were studied using the direct microscopic and anaerobic culture methods. motile curved rods were found to be three times higher in the hiv-infected group under direct microscopy. otherwise, there were no significant differences between the diseased and health ... | 2001 | 11398993 |
| inhibitory effect of lactoperoxidase-generated hypothiocyanite upon black pigmented anaerobe growth. | this study aimed to evaluate the in vitro effect of lactoperoxidase with or without its substrates (hydrogen peroxide, thiocyanate) on the growth of 4 different black pigmented anaerobe (bpa) strains associated with the development and progress of periodontal diseases: porphyromonas gingivalis atcc 33277, prevotella intermedia nctc 9336, prevotella loescheii atcc 15930, and prevotella melaninogenica nctc 9338. a 5-min lactoperoxidase-generated oscn--hoscn incubation at ph 6.0, 7.0 or 8.0 resulte ... | 2001 | 11408950 |
| evaluation of the rapid id 32a system for identification of anaerobic gram-negative bacilli, excluding the bacteroides fragilis group. | objective: to evaluate the rapid id 32a system (biomérieux, marcy-l'etoile, france) for the identification of anaerobic gram-negative bacilli, excluding the bacteroides fragilis group. methods: five hundred and twenty-eight identified clinical isolates of non-b. fragilis group anaerobic gram-negative bacilli were tested in the rapid id 32a system, and identifications were compared with those obtained with conventional biochemical tests and gas-liquid chromatography. results: the rapid id 32a sys ... | 1999 | 11856276 |
| [clinical and bacteriological features of six cases with intracranial abscess in childhood]. | from october 1988 to march 2001, 5 patients with 6 episodes of intracranial abscesses were admitted to chiba-children's hospital. average age when they were admitted was 10 years and 1 month. initial clinical symptoms were fever in 5 cases, and headache in 1 case. it took 21 days from the appearance of the initial symptoms to diagnose the intracranial abscess. four out of 5 patients had underlying diseases that were prone to cause intracranial abscess. two patients of these were cyanotic congeni ... | 2002 | 11905002 |
| antimicrobial activity of magainin analogues against anaerobic oral pathogens. | magainins are a family of potent antimicrobial cationic peptides that possess antimicrobial activity against a wide range of target organisms. in this study, the antimicrobial activity of synthetic magainin-mimetic compounds msi-751 and msi-774 was investigated against the periodontal pathogens porphyromonas gingivalis, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, eikenella corrodens, prevotella loescheii and prevotella intermedia. p. gingivalis was more susceptible to msi-751 ... | 2003 | 12507842 |
| [outbreak of tropical phagedenic ulcers after the rainy season in dijibouti]. | the incidence of cutaneous ulcers was observed after the rainy season in djibouti in 1997. based on the study of epidemiologic, clinical, biological, and therapeutic features these lesion were classified as phagedenic ulcers. while direct examination showed numerous fusiform bacilli, cultures performed in one patient, led to isolation of numerous colonies of prevotella loescheii. though less common, two other anaerobic bacterial species were detected, i.e., peptostreptococcus anaerobius and pept ... | 2003 | 12910663 |
| infection of a total hip arthroplasty with prevotella loeschii. | infection is a serious complication of total hip replacement. it has been proposed that 6% of all infections after total hip arthroplasty may be of dental origin through hematogenous spread. however, no conclusive evidence that the mouth is a definitive source for infection of a total hip replacement has been reported. in the current case, prevotella loeschii, a pigmented bacteroides species was identified in a total hip replacement. prevotella loeschii is an organism which exclusively inhabits ... | 2004 | 15043121 |
| prevotella shahii sp. nov. and prevotella salivae sp. nov., isolated from the human oral cavity. | two bacterial strains, ehs11(t) and epsa11(t), which were isolated from the human oral cavity, were characterized in terms of phenotypic and biochemical characteristics, cellular fatty acid profiles and phylogenetic position based on 16s rrna gene sequence analysis. 16s rrna gene sequence analysis showed that each of the isolates belonged to a novel species of the genus prevotella. strain ehs11(t) was related to prevotella loescheii (about 95 % similarity), whereas strain epsa11(t) was related t ... | 2004 | 15143039 |
| antimicrobial resistance and beta-lactamase production of clinical isolates of prevotella and porphyromonas species. | this study determined the beta-lactamase production and the antimicrobial resistance of 72 prevotella species and 48 porphyromonas species isolated from different clinical specimens. | 2005 | 15722627 |
| proteinase activity of prevotella species associated with oral purulent infection. | prevotella intermedia and prevotella nigrescens are often regarded as principal causes of acute dentoalveolar infection; however, other species within the genus are also known to be associated with such infection. the aim of this study was to determine the in vitro proteolytic activity of these different prevotella species that have been implicated with dentoalveolar infection. a total of 234 strains were obtained from pus specimens from dentoalveolar infection and from the plaque of healthy vol ... | 2006 | 16586023 |
| septic arthritis of the knee due to prevotella loescheii following tooth extraction. | this is a case report of septic arthritis of the knee due to prevotella loescheii, in a patient with advanced arthrosis. two weeks beforehand he had undergone a dental root extraction without antibiotic prophylaxis. his knee had become inflamed 48 hours after extraction and he was started on ibuprofen and steroid treatment (prescribed by his primary health care doctor). with a provisional diagnosis of septic arthritis, synovial fluid was taken for study. antimicrobial therapy was commenced with ... | 2008 | 18667985 |
| newer approaches in the bedside diagnosis of massive pulmonary embolism. preliminary report. | | 2016 | 5009743 |
| letter: bromide intoxication. | | 2002 | 126712 |
| frankly speaking: economics: nursing's friend or foe? | | 1999 | 96934 |
| fatal leishmaniasis in renal-transport patient. | | 1999 | 88649 |