| localization of nors in chromosomes of mouse cell lines by a combined 33258-hoechst and ag-staining technique. | | 1979 | 84805 |
| detection of a low-molecular-weight antigen on melanoma cells by a human antiserum in leukocyte-dependent antibody assays. | biochemical characterization of serologically detected human melanoma antigens was undertaken for the development of immunodiagnostic assays in melanoma. an antiserum from a human melanoma patient, which detected melanoma antigens expressed on a large proportion of different melanoma cells, was used in leucocyte-dependent cytotoxic antibody (lda) 51cr-release assays to monitor the purification of melanoma antigens in urea/acetate extracts of lactoperoxidase 125i-labelled melanoma cell membranes. ... | 1979 | 91379 |
| spontaneous interictal spiking in the awake kindled rat. | the spontaneous interictal spike (sis) noted in eeg recordings is a signal of the abnormally excitable behavior of neurons in an epileptic focus. this study provides a detailed, quantitative, temporal and spatial profile of sis in the amygdaloid kindled rat. the reproducible nature of the development and decline of sis in kindling provides a useful model for biochemical study of the mechanisms that initiate and regulate sis. | 1979 | 91486 |
| the specificity of human autoantibodies that react with both cell nuclei and plasma membranes: the nuclear antigen is present on core mononucleosomes. | we have examined the nature of the nuclear antigen recognized by certain natural human antibodies that react specifically with both cell nuclei and plasma membranes from many species. partial purification of these antibodies, called x-ana, is achieved by binding to and rapid elution from the surface of viable human leukocytes. chicken erythrocyte chromatin was solubilized by digestion with staphylococcal nuclease and fractionated into a 0.15 m nacl soluble fraction that consisted of core mononuc ... | 1979 | 91640 |
| an investigation of the optimal conditions for the in vivo production of immunologically sensitised rat mast cells. | experiments were designed to develop an optimal method for inducing in vivo production of sensitised peritoneal mast cells. rats of different strains were sensitised with whole egg-white and killed at suitable intervals to harvest the peritoneal mast cells. release of histamine was induced in vitro by both whole egg-white and its major protein constituents, and assayed by a standard spectrofluorometric method. wistar rats showed higher levels of sensitisation than black-hooded lister rats; it wa ... | 1979 | 92507 |
| the effect of dose loading and of double labelling with 57co and 125i on the tissue distribution in animals. | dose loading effects upon the performance of 57co-bleomycin as a tumor localizing agent have been investigated in rhabdomyosarcoma bearing wag/ry rats. the addition of non-radioactively labelled co-bleomycin increased the relative uptake of 57co-bleomycin in rapid growing tumors, but the addition of non-chelated bleomycin had no influence at all. in our experimental system, iodinated bleomycin generally labelled by reaction with icl, was found to be an unsatisfactory tumor localizing agent. in o ... | 1979 | 93048 |
| differential location of different types of intermediate-sized filaments in various tissues of the chicken embryo. | the location of constitutive proteins of different types of intermediate-sized (about 10 mm) filaments (cytokeratin, vimentin, desmin, brain filament protein) was examined in various tissues of 11--20 day chick embryos, using specific antibodies against the isolated proteins and immunofluorescence microscopy on frozen sections and on isolated serous membrane. the tissues studied which contained epithelia were small intestine, gizzard, esophagus, crop, liver, kidney, thymus, mesenteries, and epid ... | 1979 | 93557 |
| measurement of androgen receptor in cytosol of human prostatic tissues with a sepharose-linked antibody system. | | 1979 | 93753 |
| purification of antibodies to influenza a virus structural proteins by affinity chromatography, and their participation in haemagglutination-inhibition, neutralization and enzyme-linked immunosorbent assay. | affinity chromatography was used to purify rabbit antibodies to common and strain-specific antigeneic determinants of haemagglutinin, to neuraminidase, and to a combination of the internal proteins of influenza a viruses. the purity of the antibodies was assessed by haemagglutination-inhibition, enzyme-linked immunosorbent assay (elisa) and competition elisa. the antibodies were examined for their participation in neutralization and haemagglutination-inhibition assays, and in elisa. elisa was fo ... | 1979 | 93960 |
| an unstable donor-recipient dna complex in transformation of bacillus subtilis. | in re-extracted dna obtained shortly after uptake of transforming dna by bacillus subtilis, increased amounts of donor dna radioactivity banding at the position of donor-recipient dna complex (drc) are observed in cscl gradients, if the cells are irradiated with high doses of uv prior to reextraction of the dna. qualitatively, the same phenomenon is observed if lysates of transforming cells are irradiated. uv-irradiation of lysates of competent cells to which single-stranded dna is added after l ... | 1978 | 106232 |
| changes in lung ultrastructure following heterologous and homologous serum albumin infusion in the treatment of hemorrhagic shock. | the object of this study was to compare the ultrastructure pulmonary effects of the infusion of homologous and heterologous serum albumin solution in the treatment of hemorrhagic shock in baboons. adult baboons subjected to hemorrhagic shock were resuscitated with either baboon serum albumin, human serum albumin, or ringer's lactate solution. the lungs were fixed in vivo with potassium pyroantimony, a solution which produces electron dense interstitial precipitation of sodium. the lungs from ani ... | 1979 | 106780 |
| [radioautographic identification of dopaminergic axons in the rat cns. in vitro marking with tritiated dopamine or noradenaline]. | a new radioautographic technique for the visualization of central dopaminergic fibers is described. this technique is based upon in vitro uptake of triatiated norepinephrine or tritiated dopamine in the presence of monoamine oxidase inhibitor and specific uptake inhibitor in noradrenergic fibers. after a short step in glutaraldehyde, the use of osmium vapours as a fixative, instead of osmic acid immersion, allows a satisfactory retention of triatiated amines within dopaminergic fibers. | 1979 | 120791 |
| hydrophobic interactions of the apo-gln-i polypeptide component of human high density serum lipoprotein. | apo-gln-i, the major polypeptide component of human serum high density lipoprotein, has four noninteracting hydrophobic sites which associate with alkanes, anionic detergents, and cationic detergents. hexane and octane bind to these sites with association constants of 6.8 times 10-2 and 1.8 times 10-4 liters/mol, respectively, and compete with the anionic detergent, sodium dodecyl sulfate (c12oso3-minus), at low detergent ligand binding ratios (i.e. smaller than or equal to 1.0 mol of c12oso3-mi ... | 1975 | 165177 |
| glucagon and adipose tissue. | | 1975 | 167786 |
| the control of adaptive hypertrophy in the salt glands of geese and ducks. | 1. factors controlling adaptive hypertrophy, which occurs when marine, or potentially marine, birds drink salt water, have been investigated in geese and ducks using changes in salt-weight weight, rna and dna contents as indices of this process. 2. unilateral post-ganglionic denervation in geese prevented the changes in [rna] and [rna]:[dna] that occurred in the intact gland of birds given salt water for 24 hr; denervation had no significant effect in birds on fresh water throughout. 3. atropine ... | 1975 | 168357 |
| conditional lethal mutants of vesicular stomatitis virus. | | 1975 | 169102 |
| preferential synthesis of viral late rna by nuclei isolated from sv40 lytically infected cells. | nuclei from sv40-infected monkey cells were isolated late in lytic infection and their cell-free transcriptional activity was characterized. 3h-rna synthesized in vitro was hybridized to excess quantities of separated sv40 dna strands which were each covalently bound to sepharose. it was found that 3-5% of the newly synthesized rna is virus-specific and that the plus-strand dna, coding for late rna sequences, is transcribed at a rate about 15 times higher than that of the minus-strand dna, which ... | 1976 | 182375 |
| the transport of esterified cholesterol in plasma high density lipoproteins of human subjects: a mathematical model. | the pattern of labeling of free and esterified cholesterol in the plasma high-density lipoproteins after an intravenous injection of [3h]-mevalonic acid has been examined in six human subjects with a variety of plasma lipoprotein phenotypes. attempts have been made to fit these data to theoretical models of high-density lipoprotein esterified cholesterol transport constructed on the basis of previous experimental observation. the first model, which assumed that the high-density lipoprotein ester ... | 1976 | 184220 |
| regulation of cyclic adenosine 3',5'-monophosphate concentration in chick cerebral hemispheres during development. | | 1976 | 184253 |
| particulate carbonic anhydrase in homogenates of human kidney. | about 2% of human kidney carbonic anhydrase (carbonate hydro-lyase, ec 4.2.1.1) has been found in particulate fractions. its distribution in the particulate fractions obtained by differential centrifugation suggests that it may be concentrated in the brush border. the particulate enzyme is like red cell carbonic anhydrace c in its susceptibility to inhibition by anions. particulate carbonic anhydrase is firmly bound to the membrane and is not released by incubation at ph 10.6 and 37 degrees c or ... | 1976 | 184830 |
| [isolation of preparations of type b monoamine oxidase from liver mitochondria]. | | 1977 | 193582 |
| [possible participation of prostaglandins a1 and b1 in the development of internal carotid artery spasm]. | experiments were carried out on dogs with the internal carotid artery circulatory isolated and continuously perfused in situ with oxygenated ringer-krebs bicarbonate solution. prostaglandins a1 and b1 (pg-a1 and pg-b1) exerted a constrictory effect on the artery. the effect of both prostaglandins was significantly less, but more prolonged than that of the same doses of serotonin. a possible role of pg-a1 and pg-b1 in the development of vasospasm is testified to by the following experimental resu ... | 1977 | 195648 |
| synthesis of estradiol-17 beta by sertoli cells in culture: stimulation by fsh and dibutyryl cyclic amp. | sertoli cells isolated from the testes of 18 to 20-day old rats synthesize estradiol-17 beta when grown in primary culture in the presence of testosterone (5 x 10(-7) m). after an initial lag phase of about 2 hours, follicle-stimulating hormone (fsh) stimulates synthesis of estradiol up to 50-fold, synthesis being approximately linear for 24 hours when medium is changed every 2-6 hours. luteinizing hormone (lh) causes only a marginal stimulation, at concentrations consistent with contamination w ... | 1975 | 195777 |
| proposed effects of brain noradrenaline on neuronal activity and cerebral blood flow during rem sleep. | we propose that the observed increases of both neuronal activity and cerebral blood flow seen throughout the brain during rem sleep may be effects of decreased central noradrenaline release. | 1977 | 201486 |
| effects of compounds used for radiostrontium removal on 85sr distribution in diffusible & non-diffusible forms in rat tissues. | | 1977 | 202563 |
| acute encephalopathy with liver dysfunction, chylous ascites and cytomegalovirus infection. | a child with acute encephalopathy and liver dysfunction subsequently developed acute chylous ascites. titers for cytomegalovirus increased from less than 1:2 to 1:32 during the illness, and cytomegalovirus was isolated from the urine. the case is the first one possibly linking cytomegalovirus and acute encephalopathy and liver dysfunction in a child. in our patient, enlargement of the abdominal lymph nodes, as seen on a lymphangiogram, resulted in a severe obstruction of abdominal lymphatic flow ... | 1977 | 202566 |
| dissociation of cyclic gmp from the negative inotropic action of carbachol in guinea pig atria. | the relationship between the negative inotropic action of carbachol and its ability to elevate cyclic gmp was determined in isolated paced guinea pig atria. a clear dissociation was observed between that concentration of carbachol which depressed contractility and that which elevated tissue cyclic gmp content. doses as low as 0.03 micronm caused a negative inotropic effect while cyclic gmp was not elevated until concentrations nearly 100-fold higher were used. thus a correlation between tissue c ... | 1977 | 203613 |
| intestinal calcium binding protein levels in the one month old turkey poult. | intestinal calcium binding protein cabp) was studied in one month old poults which had been fed a vitamin d3-deficient diet unsupplemented or with 399, 600, 900 or 1200 i.u. vitamin d3 added per kg. diet. cabp levels increased with vitamin d3 supplementation up to 600 i.u. but showed no further increase with higher dietary vitamin d3. poults supplemented with 600 to 1200 i.u. vitamin d3/kg. diet had an average of 1.03 mg. cabp/g. of duodenal mucosa cells collected. the calcium binding activity o ... | 1977 | 203920 |
| the effect of cyclic amp on the maturation and degranulation of mast cells. | in short-term tissue cultures dibutyryl camp inhibits the cortisone-induced degranulation of mast cells. the effect of methylxanthine was found to be similar but developed at a slower rate. the increase of camp level also affected the maturation of mast cells, as the ratio of cells of mixed granulation increased, compared to the alcian blue- and safranin-present model inhibited degranulation. | 1977 | 204137 |
| a model of quick phase generation in the vestibuloocular reflex. | the vestibuloocular relfex of the cat was studied during step and sinusoidal head velocity stimuli. a model is presented which simulates the observed slow phase and quick phase behavior. the model is constructed to be compatible with neurophysiological observations of the behavior of neurons in the pons. emphasis is placed on the amplitude and timing of quick phases which are active orienting movements that drive the eyes into the direction of turning. it is proposed that quick phases, like sacc ... | 1978 | 204366 |
| [invasion of the mucosa of the colon by entamoeba histolytica. recent observations]. | | 1978 | 213335 |
| a protein from ustilago which forms an acid-soluble complex with deoxyribonucleic acid. | a protein which can render dna largely acid-soluble has been purified 1600-fold from high salt extracts of ustilago maydis. the activity is unusual in that substrate dna is not made acid-soluble through hydrolysis to small oligomers. rather, the protein appears to bind to dna to form a complex which itself is acid-soluble. the activity of conversion of dna to an acid-soluble form is cold-labile, but the inactivation by cold is reversible by brief heat treatment. divalent cations stimulate the ac ... | 1975 | 235535 |
| the establishment of bacteroides ruminicola in a gnotobiotic lamb. | | 1975 | 237060 |
| purification and partial characterization of deoxyribonuclease i from bovine parotid gland. | deoxyribonuclease i has been purified from bovine parotid gland. the purification procedure utilizes an acid extraction of minced parotid gland, salt fractionation, gel filtration, and ion-exchange chromatography. the last step, chromatography on sulfopropyl-sephadex, resolves the enzymatic activity into several fractions. the major fraction, designated dnase a, was subjected to further investigation. this enzyme has, as expected, an alkaline ph optimum and an obligate requirement for divalent c ... | 1977 | 265962 |
| taste acuity of the human palate. iii. studies with taste solutions on subjects in different age groups. | the taste acuity at the midline of the hard and soft palate near their junction and, for comparison, on representative areas of the tongue was determined in 80 subjects aged 11-79 years by applying test solutions of the four basic tastes. twenty-one subjects (26%) could identify at least one taste on the hard palate but none could recognize all four tastes. seventy subjects (87%) could identify at least one taste on the soft palate and 37 subjects (46%) could recognize all four tastes. taste thr ... | 1979 | 291279 |
| d-penicillamine: mitogenic effect on mouse, rat and human spleen lymphocytes. | the effect of d-penicillamine (d-pen) on the proliferation of cultures of normal mouse, rat, and human spleen lymphocytes and peripheral blood lymphocytes was examined. d-pen in concentrations of 2 x 10(-3) m to 8 x 10(-3) m in serum-free and in serum-containing medium resulted in a highly significant incorporation of 3h-tdr by normal mouse and rat spleen cells. enhanced incorporation of 3h-tdr by normal human spleen cells only occurred in serum-containing medium. d-pen in concentrations of 10(- ... | 1979 | 315028 |
| flow cytometry of isolated nuclei prepared from 9l rat brain tumor. | nuclei isolated by grinding 9l rat brain tumors gave a dna distribution consisting of a 2c dna peak due to noncycling normal cells and a bimodal distribution beginning at about 4c dna due to the cycling tumor cells. autoradiographic studies of nuclei from rats that received a pulse of 3h-tdr in vivo confirmed that the majority of proliferating cells were in the bimodal part of the distribution. the fraction of cells in s-phase determined from the dna distributions was 15.3 per cent. this is cons ... | 1979 | 376940 |
| an inducible proline transport system in candida albicans. | 1. when candida albicans cells were preincubated with proline or grown in the presence of proline as the sole nitrogen source they exhibited a rapid increase in the influx of proline (the inducible transport system). 2. the induction appeared to be specific for proline and also demonstrated in other candida species. 3. both the inducible and constitutive proline uptake systems exhibited similar characteristic features. 4. the nature of the inducer for proline uptake in c. albicans appeared to be ... | 1979 | 383150 |
| some effects of uncouplers and inhibitors on growth and electron transport in rumen bacteria. | uncouplers and inhibitors of electron transport affected growth and electron transport of rumen bacteria in various ways. selenomonas ruminantium was not affected by inhibitor and uncoupler concentrations which affected growth and electron transport of bacteroides ruminicola, b. succinogenes, and butyrivibrio fibrisolvens. inhibitors, when active, led to accumulation of reduced electron carriers before the site of action, but differences were found among organisms in the site of action of these ... | 1979 | 457609 |
| xylose, arabinose, and rhamnose fermentation by bacteroides ruminicola. | metabolism and growth yields of bacteroides ruminicola grown on d-xylose, l-arabinose, and l-rhamnose were studied. growth yields were 62, 68, and 35.5 g (dry weight) per mol of carbohydrate fermented after correction for storage polysaccharide. experiments with [1-(14)c]arabinose indicated that pentose was fermented by a pentose phosphate cycle plus glycolysis, with some indication of a minor phosphoketolase-type pathway. the product ratios from pentose were similar to those previously describe ... | 1979 | 485153 |
| obligate anaerobes in clinical veterinary practice. | clinical specimens obtained from domestic animals were examined to determine the relative prevalence of obligate anaerobic bacteria and the species represented. of 3,167 samples cultured anaerobically as well as aerobically, 2,234 were bacteriologically positive. of these positive samples, 583 (26%) contained species of obligate anaerobic bacteria in a total of 641 isolates. most positive samples contained anaerobes admixed with aerobic species, although 6% of such samples yielded pure cultures ... | 1979 | 511987 |
| deoxyribonucleic acid base composition of certain species of the genus bacteroides. | the moles percent guanine plus cytosine content of the dna (% g + c) of 15 bacteroides strains representing six species was determined. one group, including three strains of bacteroides ruminicola, two strains of b. melaninogenicus, two strains of b. succinogenes, and one strain of b. oralis (ji), had a % g + c of 47.6--50.3 and a second group including two strains of b. amylophilus, four strains of b. fragilis, and one strain of b. succinogenes had a lower % g + c of 40.3--42.7. the taxonomic r ... | 1977 | 561646 |
| alkaline phosphatase activity of rumen bacteria. | of the 54 strains of rumen bacteria examined for alkaline phosphatase (apase) production, 9 of 33 gram-negative strains and none of 21 gram-positive strains produced the enzyme. the apase of the cells of the three strains of bacteroides ruminicola that produced significant amounts of the enzyme was located in the periplasmic area of the cell envelope, whereas the enzyme was located in the strains of selenomonas ruminantium and succinivibrio dextrinosolvens was associated with the outer membrane. ... | 1977 | 563216 |
| production of branched-chain volatile fatty acids by certain anaerobic bacteria. | net production of isobutyric acid, isovaleric acid, and 2-methylbutyric acid by cultures of bacteroides ruminicola and megasphaera elsdenii on media that contained trypticase or casein hydrolysate continued (up to 5 days) after growth had ceased. only trace quantities of these acids were produced in a medium that contained a mixture of amino acids that did not include the branched-chain amino acids. m. elsdenii produced increased quantities of the branched-chain fatty acids in a medium that cont ... | 1978 | 566082 |
| amino acid uptake systems in bacteroides ruminicola. | uptake of amino acids by bacteroides ruminicola was observed in cells grown in a complete defined medium, containing ammonia as the nitrogen source. a high rate of uptake occurred only in fresh medium, as an inhibitory substance, possibly acetate, apparently accumulated during growth. all amino acids except proline were taken up and incorporated into cold trichloroacetic acid precipitable material. different patterns of incorporation and different responses to 2,4-dinitrophenol and potassium fer ... | 1979 | 575310 |
| origins of fermentation products formed during growth of bacteroides ruminicola on glucose. | bacteriodes ruminicola grown on complex medium with glucose as carbon source gave acetate, co2, formate and succinate as main fermentation products. no evidence was found for significant glucose catabolism by pathways other than the embden-meyerhof sequence. however, [u-14c]glucose fermentation gave products whose specific radioactivities were much lower than expected. there appear to be two main causes. firstly, a rapid exchange occurred between metabolic intermediates and co2, probably due to ... | 1978 | 670931 |
| inhibitory effects of h2 on growth of clostridium cellobioparum. | hydrogen inhibits the growth of hydrogen-producing clostridium cellobioparum, but not of escherichia coli or bacteroides ruminicola. the inhibition is reversible. when hydrogen was removed either by palladium black or by gassing out the tube, glucose utilization increased as did optical density and hydrogen production of c. cellobioparum. removal of the h2 by methanogenic bacteria favors the growth of c. cellobioparum. grown with methanobacterium ruminantium in various concentrations of glucose, ... | 1976 | 779644 |
| characterization of the predominant bacteria occurring in the rumen of goats (capra hircus). | a total of 44 strains of bacteria were isolated from rumen contents of the goat. based on morphology, gram stain, anaerobiosis, motility, and fermentation end products, they were grouped into 11 different types. for each type, all or representative strains were characterized in detail. the type, number of strains characterized over total number of strains, and identification were as follows: type 1, 6/21, atypical butyrivibrio fibriosolvens; type 2, 6/9, atypical butyrivibrio fibrisolvens; type ... | 1977 | 879766 |
| urease assay and urease-producing species of anaerobes in the bovine rumen and human feces. | a growth medium and test were developed for rapid detection of urease in fermentative anaerobic bacteria. using nonselective rumen fluid roll-tube agar medium and the new test, it was confirmed that peptostreptococcus productus is often the most numerous urease-forming species in human feces. also, some fecal strains of ruminococcus albus, clostridium innocuum, and clostridium beijerinckii produced urease. single strains of fusobacterium prausnitzii, coprococcus catus, and streptococcus mitis th ... | 1977 | 879770 |
| tetrapyrrole utilization by bacteroids ruminocola. | reduced versus oxidized difference spectra of whole cells and pyridine hemochromogens of heme-requiring isolates of bacteroides ruminicola are altered when deuteroporphyrin or mesoporphyrin replaces protoheme as a growth factor. during growth in the presence of either deuteroporphyrin or mesoporphyrin, whole cells exhibit peaks at 545 t547, 515 to 518, and 412 to 413 nm. pyridine hemochromogen spectra confirm the presence of meso -or deuteroheme in cells grown in the presence of meso- or deutero ... | 1977 | 893342 |
| [the bacteriodaceae flora in the faeces of pigs (author's transl)[]. | a total of 180 bacteroidaceae strains from the fecal specimens of 31 clinically healthy pigs aged 120 to 180 days from 4 farms were isolated in numbers of 10(8)-10(10) per gram feces and differentiated for their morphological, cultural and biochemical properties. 161 of these strains belonged to bacteroides ruminicola and related species, 10 to b. fragilis ss. fragilis, 3 to b. fragilis ss. vulgatus, 4 to fusobacterium necrogenes, 1 to f. mortiferum and 1 to a non-motile variant of f. novum. the ... | 1976 | 936825 |
| metabolism and growth yields in bacteroides ruminicola strain b14. | metabolism of d-glucose by bacteroides ruminicola subsp. brevis, strain b14, has been examined. growth yield studies gave molar growth yields, corrected for storage polysaccharide, of approximately 66 g (dry weight)/mol of glucose fermented. the storage polysaccharide amounted to about 14% of the total dry weight, or 55% of the total cellular carbohydrate, at full growth. after correcting glucose utilization for incorporation into cellular carbohydrate, measurement of product formation showed th ... | 1976 | 970946 |
| effect of actinomycin d and oxygen on the ribonucleic acid synthesis of an anaerobic gram-negative bacterium. | the synthesis of ribonucleic acid by whole cells of bacteroides ruminicola is not sensitive to actinomycin d, but it is sensitive to actinomycin d in the presence of ethylenediaminetetraacetate. ribonucleic acid synthesis by whole cells of this gram-negative anaerobic bacterium is also totally inhibited by oxygen. | 1975 | 1147598 |
| fermentation products in the rumen of a gnotobiotic lamb dosed with bacteroides ruminicola. | | 1975 | 1187635 |
| glucose toxicity and inability of bacteroides ruminicola to regulate glucose transport and utilization. | ammonia-limited (3.5 mm ammonia) cultures of bacteroides ruminicola b(1)4 had a high number of viable cells (greater than 10(9)/ml), but only when the concentration of glucose was not too high (10 mm or less). when the glucose concentration was increased from 10 to 50 mm, there was a marked decrease in viability (10(5)-fold or greater). because there was little decline in ph and only a small increase in succinate and acetate as the glucose concentration was increased, it did not appear that end ... | 1992 | 1320369 |
| presynaptic dopaminergic inhibition of the spinal reflex in rats. | dopaminergic influence on spinal monosynaptic transmission was examined in rats. monosynaptic mass reflex (mmr) was recorded from the ventral root l6 following supramaximal stimulation (0.2 hz; 0.1 ms) to the ipsilateral dorsal root l6 in spinalized rat under pentobarbitone sodium (40 mg/kg, i.p.) anaesthesia. mmr was inhibited by intravenous administration of the dopaminergic agonist, apomorphine (50-200 ug/kg) in a dose-dependent manner. the attenuatory effect of apomorphine (200 ug/kg i.v.) o ... | 1992 | 1352179 |
| deoxyribonuclease activity in rumen bacteria. | deoxyribonuclease activity was surveyed in 22 strains belonging to 12 species of rumen bacteria, with lambda bacteriophage dna as substrate. activity was readily detected in broken cell preparations from 15 of these strains. particularly high levels of activity were present in cells and culture supernatant of all 5 strains of bacteroides succinogenes, and 2 out of 6 strains of bacteroides ruminicola, examined. | 1990 | 1366671 |
| [gastric emptying of solids, acid secretion and tobacco in duodenal ulcer]. | we study in a group of patients with endoscopically diagnosed duodenal ulcer (19; 17 males) and controls (11; 7 males) the gastric emptying of solids through scintigraphy and gastric acid secretion by standard tests. in the same way we investigated prospectively some clinical data, specially smoking habits. as a whole, patients with duodenal ulcer showed an emptying of solids slightly faster than controls (t 1/2-minutes-: 85.4 +/- 28.6 in patients with duodenal ulcer versus 116.9 +/- 46.5 in con ... | 1992 | 1389553 |
| [the characteristics and regulation of glutamate dehydrogenase in strains of bacteroides ruminicola ssp. ruminicola isolated from the rumen of fallow deer]. | very little information about nh4+ assimilation paths in rumen anaerobic bacteria is available, and the information about wild animals is completely missing. glutamate dehydrogenase (gdh) isolated from the rumen strain b. ruminicola in fallow deer was purified and its properties were specified after crystalline ammonium sulphate precipitation and gel filtration on sephadex g-200. the properties of partly purified gdh were specified. one of the first specifications concerning gdh from various sou ... | 1992 | 1413388 |
| properties of a genetically reconstructed prevotella ruminicola endoglucanase. | a puc19-derived plasmid was constructed that coded for a hybrid cellulase with the thermomonospora fusca e2 cellulose-binding domain at its c terminus joined to the prevotella ruminicola 40.5-kda carboxymethyl cellulase (cmcase). the hybrid enzyme was purified and characterized enzymatically. it bound tightly to cellulose, and its specific activities on carboxymethyl cellulose, amorphous cellulose, and ball-milled cellulose were 1.5, 10, and 8 times that of the 40.5-kda cmcase, respectively. fur ... | 1992 | 1482181 |
| distribution of xylanase genes and enzymes among strains of prevotella (bacteroides) ruminicola from the rumen. | the distribution of two xylanase genes was examined by southern hybridization among 26 strains of the rumen anaerobic bacterium prevotella (bacteroides) ruminicola. hybridization with a xylanase/endoglucanase gene from the type strain 23 was found in six strains while hybridization with a xylanase gene from strain d31d was found in 14 strains. sequences related to both genes were present, on different restriction fragments, in six strains, whereas no hybridization to either gene was detected in ... | 1992 | 1490595 |
| selective isolation of bacteria with dipeptidyl aminopeptidase type i activity from the sheep rumen. | five-hundred-and-six fresh isolates of rumen bacteria were tested for their ability to hydrolyse the synthetic substrate for dipeptidyl aminopeptidase type i, glyarg-4-methoxy-2-naphthylamide (glyarg-mna), using a gel overlay technique. twelve positive isolates were small gram-negative rods which resembled bacteroides ruminicola in their biochemical and morphological properties. sds-page of whole cell extracts indicated that two were similar to b. ruminicola strain b14, six resembled b. ruminico ... | 1992 | 1526449 |
| interaction of ruminal bacteria in the production and utilization of maltooligosaccharides from starch. | the degradation and utilization of starch by three amylolytic and one nonamylolytic species of ruminal bacteria were studied. pure cultures of streptococcus bovis jb1, butyrivibrio fibrisolvens 49, and bacteroides ruminicola d31d rapidly hydrolyzed starch and maltooligosaccharides accumulated. the major starch hydrolytic products detected in s. bovis cultures were glucose, maltose, maltotriose, and maltotetraose. in addition to these oligosaccharides, b. fibrisolvens cultures produced maltopenta ... | 1992 | 1539992 |
| properties of ionophore-resistant bacteroides ruminicola enriched by cultivation in the presence of tetronasin. | bacteroides ruminicola m384 was grown in the presence of increasing concentrations of tetronasin, an ionophore that has been developed as a feed additive for ruminants. the resulting culture, b. ruminicola m384/tnr, was then maintained in medium containing 0.1 microgram tetronasin/ml. growth of the parent strain was eliminated by the addition of 0.1 micrograms tetronasin/ml, but the growth rate of b. ruminicola m384/tnr, which grew more slowly than the parent strain, was unaffected by adding tet ... | 1992 | 1541602 |
| evidence for natural transfer of a tetracycline resistance gene between bacteria from the human colon and bacteria from the bovine rumen. | previously, we demonstrated conjugal transfer of a specially constructed shuttle vector, prdb5, from the human colonic anaerobe bacteroides uniformis to the ruminal anaerobe prevotella (bacteroides) ruminicola b(1)4. we have now shown that naturally occurring gene transfer elements in bacteroides species and prevotella ruminicola can also be transferred between these two genera. a self-transmissible chromosomal element originally found in a clinical isolate of bacteroides fragilis (tcr emr 12256 ... | 1992 | 1599250 |
| dna sequence and transcription of an endoglucanase gene from prevotella (bacteroides) ruminicola ar20. | the endoglucanase gene was sequenced from prevotella ruminicola ar20, isolated as clone pjw4. the endoglucanase (brend) is encoded by an open reading frame (orf1) of 501 codons, corresponding to a protein of calculated molecular weight 55.7 kda. analysis of proteins on sds-page revealed a protein corresponding to the calculated molecular weight of the processed brend. the protein showed substantial homology to members of the a4 sub-family cellulases. primer extension studies revealed that transc ... | 1992 | 1603069 |
| some growth and metabolic characteristics of monensin-sensitive and monensin-resistant strains of prevotella (bacteroides) ruminicola. | new strains with enhanced resistance to monensin were developed from prevotella (bacteroides) ruminicola subsp. ruminicola 23 and p. ruminicola subsp. brevis ga33 by stepwise exposure to increasing concentrations of monensin. the resulting resistant strains (23mr2 and ga33mr) could initiate growth in concentrations of monensin which were 4 to 40 times greater than those which inhibited the parental strains. resistant strains also showed enhanced resistance to nigericin and combinations of monens ... | 1992 | 1622231 |
| vitamin b12-dependent propionate production by the ruminal bacterium prevotella ruminicola 23. | when prevotella ruminicola 23 was grown in a defined medium containing a vitamin mixture, significant amounts of propionate were formed. succinate and acetate were the major fermentation acids produced when vitamins were omitted, and further experiments demonstrated that propionate formation was dependent on vitamin b12. when the organism was grown in continuous culture at dilution rates of less than 0.20 h-1, propionate and acetate were the predominant fermentation products and little succinate ... | 1992 | 1637169 |
| enzymes associated with metabolism of xylose and other pentoses by prevotella (bacteroides) ruminicola strains, selenomonas ruminantium d, and fibrobacter succinogenes s85. | prevotella (bacteroides) ruminicola strains b(1)4 and s23 and selenomonas ruminantium strain d used xylose as the sole source of carbohydrate for growth, whereas fibrobacter succinogenes was unable to metabolize xylose. prevotella ruminicola strain b(1)4 exhibited transport activity for xylose. in contrast, f. succinogenes lacked typical xylose uptake activity but did exhibit low binding potential for the sugar. prevotella ruminicola strains b(1)4 and s23 as well as s. ruminantium d showed low x ... | 1992 | 1643581 |
| ion chromatographic methods for the detection of starch hydrolysis products in ruminal digesta. | dionex high-performance ion chromatographic methods were evaluated for separation and quantitation of plant sugars and starch digestion products in the ruminal digesta of cattle. mono- and disaccharides were eluted from a dionex carbopac pa1 column with sodium hydroxide used isocratically or as a ph gradient. maltooligosaccharides which had a degree of polymerization (dp) less than 30 glucose residues were eluted in 60 min by a sodium hydroxide eluent containing a sodium acetate gradient. carboh ... | 1991 | 1653257 |
| utilization of nucleic acids by selenomonas ruminantium and other ruminal bacteria. | species of ruminal bacteria were screened for the ability to grow in media containing rna or dna as the energy source. bacteroides ruminicola d31d and selenomonas ruminantium hd4, ga192, and d effectively used rna for growth, but not dna. b. ruminicola d31d was able grow on nucleosides but not on bases or ribose. the s. ruminantium strains were able to grow when provided with either nucleosides or ribose but not bases. strains of s. ruminantium, but not b. ruminicola d31d, were also able to use ... | 1990 | 1707252 |
| taxonomic relationships among strains of the anaerobic bacterium bacteroides ruminicola determined by dna and extracellular polysaccharide analysis. | dna and extracellular polysaccharide (eps) analyses were performed on 14 strains of bacteroides ruminicola. the guanine-plus-cytosine (g+c) base contents, determined from the buoyant densities of chromosomal dnas, showed a broad range of values, from 37.6 to 50.9 mol%. dna hybridization showed generally low dna relatedness among the strains. seven strains formed two groups of closely related bacteria consisting of five (group 1) and two (group 2) strains, and another strain, e42g, showed moderat ... | 1991 | 1746957 |
| a survey of peptidase activity in rumen bacteria. | twenty-nine strains of 14 species of rumen bacteria were screened for their ability to hydrolyse ala2, ala5, glyarg-4-methoxy-2-naphthylamide (glyarg-mna) and leu-mna. several species, notably megasphaera elsdenii, were active against ala2, and a smaller number, including bacteroides ruminicola, butyrivibrio fibrisolvens, ruminococcus flavefaciens, lachnospira multipara and ruminobacter amylophilus, broke down ala5. streptococcus bovis had an exceptionally high leucine arylamidase activity. howe ... | 1991 | 1748877 |
| conjugal transfer of a shuttle vector from the human colonic anaerobe bacteroides uniformis to the ruminal anaerobe prevotella (bacteroides) ruminicola b(1)4. | prevotella ruminicola (formerly bacteroides ruminicola) is an anaerobic, gram-negative, polysaccharide-degrading bacterium which is found in the rumina of cattle. since p. ruminicola is thought to make an important contribution to digestion of plant material in rumina, the ability to alter this strain genetically might help improve the efficiency of rumen fermentation. however, previously there has been no way to introduce foreign dna into p. ruminicola strains. in this study we transferred a sh ... | 1991 | 1768083 |
| the anti-tachycardic mechanism of a direct-acting vasodilator, budralazine, in rats. | the present study was undertaken to elucidate the anti-tachycardic effect of a direct-acting vasodilator, budralazine, using an electrophysiological technique. normotensive male wistar rats were used. rats were anesthetized intraperitoneally with urethane and alpha-chloralose. intravenous administration of budralazine (0.5-5.0 mg/kg) produced a dose-dependent reduction of mean arterial pressure in anesthetized rats. at doses of 0.5 and 1.0 mg/kg, budralazine induced bradycardia accompanied with ... | 1991 | 1773498 |
| intracellular ph of acid-tolerant ruminal bacteria. | acid-tolerant ruminal bacteria (bacteroides ruminicola b1(4), selenomonas ruminantium hd4, streptococcus bovis jb1, megasphaera elsdenii b159, and strain f) allowed their intracellular ph to decline as a function of extracellular ph and did not generate a large ph gradient across the cell membrane until the extracellular ph was low (less than 5.2). this decline in intracellular ph prevented an accumulation of volatile fatty acid anions inside the cells. | 1991 | 1781695 |
| growth arrest induced by transforming growth factor beta 1 is accompanied by protein phosphatase activation in human keratinocytes. | protein phosphorylation and dephosphorylation are involved in regulation of cell growth. we tested the hypothesis that the growth inhibitory effect of transforming growth factor beta 1 (tgf-beta 1) involves activation of protein phosphatases. exposure of human keratinocytes in culture to 400 pm tgf-beta 1 for 48 h led to 80% inhibition of dna synthesis as measured by nuclear labeling. incubation of cultured keratinocytes with 400 pm tgf-beta 1 rapidly activated (within 30 min) protein serine/thr ... | 1991 | 1847373 |
| a bacteroides ruminicola 1,4-beta-d-endoglucanase is encoded in two reading frames. | escherichia coli transformed with a plasmid containing a bacteroides ruminicola endoglucanase (carboxymethyl cellulase [cmcase]) gene produced three immunologically cross-reacting cmcases which had molecular weights of 40,500, 84,000, and 88,000, while b. ruminicola produced cmcases with molecular weights of 82,000 and 88,000. the two b. ruminicola enzymes (purified from culture supernatants) had different n-terminal amino acid sequences, but each enzyme was encoded by the same gene (three indep ... | 1991 | 1938895 |
| the hydrolysis of lucerne cell-wall monosaccharide components by monocultures or pair combinations of defined ruminal bacteria. | the defined ruminal bacterial strains fibrobacter succinogenes s85, ruminococcus flavefaciens fd1, ruminococcus albus 7, butyrivibrio fibrisolvens d1, and bacteroides ruminicola ga33 were grown, in monocultures or as combinations of pair strains, on isolated lucerne cell-walls (cw) as the sole carbohydrate substrate. fibrobacter succinogenes s85 was the dominant strain determining extent of cw hydrolysis in all combinations with s85. the hydrolysis of cellulose, xylan, hemicellulose side-sugars, ... | 1991 | 2030098 |
| introduction of the bacteroides ruminicola xylanase gene into the bacteroides thetaiotaomicron chromosome for production of xylanase activity. | the xylanase gene from the ruminal bacterium bacteroides ruminicola 23 is highly expressed in colonic bacteroides species when carried on plasmid pval-rx. in order to stabilize xylanase expression in the absence of antibiotic selection, the xylanase gene was introduced into the chromosome of bacteroides thetaiotaomicron 5482 by using suicide vector pval-7. xylanase activity in the resulting strain, b. thetaiotaomicron btx, was about 30% of that observed in b. thetaiotaomicron 5482 containing the ... | 1991 | 2036016 |
| plasmid transformation of bacteroides spp. by electroporation. | transformation of bacteroides spp. with a variety of plasmid dnas was accomplished using electroporation. the standard transformation assay system used to deduce the optimal electroporation parameters employed a 50-to 100-fold concentrated cell suspension of mid-logarithmic phase bacteroides fragilis strain 638 and the 5.4-kb clindamycin resistance (ccr) vector, pbi191. a variety of electroporation buffers were used successfully in transformation experiments but of these, 1 mm mgcl2 in 10% glyce ... | 1990 | 2096397 |
| site-specific rate constants for iron removal from diferric transferrin by nitrilotris(methylenephosphonic acid) and pyrophosphate. | the kinetics of iron removal from human serum diferric transferrin by nitrilotris(methylenephosphonic acid) (ntp) and pyrophosphate (ppi) have been studied in 0.1 m, ph 7.4, n-(2-hydroxyethyl)piperazine-n'-2-ethanesulfonate buffer at 25 degrees c using urea/polyacrylamide gel electrophoresis. the four microscopic rate constants required for a complete description of iron removal from the two transferrin metal-binding sites have been measured at 100 mm concentrations of ntp and ppi. there is very ... | 1990 | 2168158 |
| cloning of the yeast sfl2 gene: its disruption results in pleiotropic phenotypes characteristic for tup1 mutants. | we have identified a yeast gene, sfl2 (suppressor gene for flocculation), which complemented a newly isolated sfl2 mutant. this mutation causes asexual cell aggregation. the strain bearing the sfl2 gene disruption exhibited pleiotropic phenotypes characteristic for tup1 mutants. physical mapping and complementation analysis suggested that the cloned sfl2 gene is identical to the tup1 gene. the sfl2 gene encodes a 669-amino acid protein which has domains rich in glutamine, as does the ssn6 protei ... | 1990 | 2197185 |
| [denture stomatitis. its relation to candida albicans]. | one of the most usual complications of the patients that carry a prosthesis is one called stomatitis denture. its etiology is not clear enough. in this work we study ninety people, sixty of them wear prosthesis. we take samples of different places in the mouth to plate and class c. albicans. we look for the relationship of results with the clinical parameters of the patient. | 1990 | 2203365 |
| non-immune hydrops in trisomy-18. diagnosis by vaginosonography and chorionic villus sampling in the first trimester. case report. | | 1990 | 2223690 |
| obligately anaerobic bacterial species isolated from foot-rot lesions in goats. | lesions showing clinical signs of foot-rot from 120 goats were cultured on six selective media during october 1987 to november 1988. a total of 582 strictly anaerobic microorganisms belonging to 50 different species was isolated and identified. the anaerobes most frequently isolated belonged to the following genera: bacteroides (80%), peptostreptococcus (63.6%), megasphaera (40%), fusobacterium (29.2%), clostridium (22.5%), propionibacterium (12.5%), eubacterium (11.7%) and leptotrichia (10.8%). ... | 1990 | 2271912 |
| physiology and genetics of xylan degradation by gastrointestinal tract bacteria. | hemicelluloses or xylans are major components (35%) of plant materials. for ruminant animals, about 50% of the dietary xylans are degraded, but only small amounts of xylans are degraded in the lower gut of nonruminant animals and humans. in the rumen, the major xylanolytic species are butyrivibrio fibrisolvens and bacteroides ruminicola. in the human colon, bacteroides ovatus and bacteroides fragilis subspecies "a" are major xylanolytic bacteria. xylans are chemically complex, and their degradat ... | 1990 | 2283426 |
| heterologous expression of the bacteroides ruminicola xylanase gene in bacteroides fragilis and bacteroides uniformis. | a cloned xylanase gene from the ruminal bacterium bacteroides ruminicola 23 was transferred by conjugation into the colonic species bacteroides fragilis and bacteroides uniformis by using the escherichia coli-bacteroides shuttle vector pval-1. the cloned gene was expressed in both species, and xylanase specific activity in crude extracts was found to be at least 1400-fold greater than that found in the b. ruminicola strain. analysis of crude extract proteins from the recombinant b. fragilis by s ... | 1990 | 2323546 |
| effects of the ionophore tetronasin on nitrogen metabolism by ruminal microorganisms in vitro. | the effects of tetronasin on ruminal protein metabolism were investigated in vitro using ruminal fluid from cattle receiving tetronasin in the diet, ovine ruminal fluid from animals not receiving tetronasin and pure cultures of proteolytic ruminal bacteria. ruminal fluid from cattle receiving tetronasin in a predominantly barley diet had lower proteolytic (76% of control, p less than .10) and deaminative (58% of control, p less than .05) activities than controls after 42 d. the effect of deamina ... | 1990 | 2332385 |
| cloning and sequencing of a bacteroides ruminicola b(1)4 endoglucanase gene. | bacteroides ruminicola b(1)4, a noncellulolytic rumen bacterium, produces an endoglucanase (carboxymethylcellulase [cmcase]) that is excreted into the culture supernatant. cultures grown on glucose, fructose, maltose, mannose, and cellobiose had high specific activities of cmcase (greater than 3 mmol of reducing sugar per mg of protein per min), but its synthesis was repressed by sucrose. b. rumincola did not grow on either ball-milled or acid-swollen cellulose even though the cmcase could hydro ... | 1990 | 2361940 |
| the survival of subgingival plaque bacteria in an amine fluoride-containing gel. | subgingival plaque samples from 20 patients with chronic inflammatory periodontal disease were exposed to a commercial gel formulation containing 2 amine fluorides. the mic of the gel for these samples ranged from 33 to 260 micrograms/ml with a modal value of 260 micrograms/ml. in each sample, the most resistant organisms (i.e., those organisms surviving at one doubling dilution below the mic) were identified. 33 such organisms were isolated, of which 22 (67%) were strict anaerobes, and 25 (75%) ... | 1990 | 2387913 |
| amylolytic activity of selected species of ruminal bacteria. | a variety of species of ruminal bacteria were screened for the ability to grow in starch-containing medium and produce amylase. of those tested, the highest levels of amylase were produced by streptococcus bovis jb1 and ruminobacter amylophilus h18. other strains that grew well on starch and produced amylase included butyrivibrio fibrisolvens a38 and 49 and bacteroides ruminicola 23 and b14. varying the carbohydrate source provided for growth resulted in changes in the growth rate and level of a ... | 1988 | 2454075 |
| [anaerobic bacteria in intracranial suppuration]. | in four cases of intracranial suppurative disease, studied in the laboratory of anaerobic bacteria of institute pasteur of algiers, three cases are caused by anaerobic bacteria and one remain sterile. two of them are empyema with unknown origin and the others are brain abscess with bilateral chronic ear infection to one. in the three cases, gram negative anaerobic bacilli were grown in culture: bacteroides asaccharolyticus, bacteroides vulgatus and bacteroides ruminicola brevis. in two cases, th ... | 1989 | 2489404 |
| [effect of systemic oral administration of tetracycline on experimental gingivitis in golden hamsters]. | local and systemic administrations of tetracycline have been used in human periodontal treatment for conditions including juvenile periodontitis and rapidly progressive periodontitis, although microbiological effects of the treatment have not been clear. the effect of systemic oral administration of tetracycline on subgingival bacteria in experimental periodontal disease in hamsters as an animal model has not yet been reported. the aim of this study was to investigate changes in subgingival bact ... | 1989 | 2489558 |
| morphological response of mycoplasma to fleroxacin. | quinolones exhibit partially reduced activity in vitro against genital mycoplasma. therefore, the morphological response to fleroxacin, a difluorinated quinolone, by mycoplasma hominis was studied. even at subinhibitory concentrations the ultrastructural investigations revealed distinct alterations such as cytolysis, vacuole and cell "ghost" formation. the typical dumbbell-shaped cells observed with monofluorinated compounds were not found, which may indicate a qualitatively different mode of ac ... | 1989 | 2515157 |
| specificity spillover at the hormone receptor--exploring its role in human disease. | | 1989 | 2537464 |
| electroporation induced transformation of bacteroides ruminicola and bacteroides uniformis by plasmid dna. | frequencies of greater than 10(5) transformants per microgram dna were achieved in bacteroides ruminicola f101 by electroporation of cells under anaerobic conditions, using the 19.5 kbp tetracycline resistance plasmid prri4. similar procedures gave frequences of 10(6) erythromycin resistant transformants per microgram dna with the shuttle plasmid pdp1 (19 kbp) in bacteroides uniformis. transformation of b. uniformis occurred at a far lower frequency (10(3) micrograms) when pdp1 dna was derived f ... | 1989 | 2599352 |
| isolation of an endoglucanase gene from bacteroides ruminicola subsp. brevis. | a gene coding for endo-1, 4-beta-glucanase activity has been isolated from bacteroides ruminicola subsp. brevis by cloning in escherichia coli. after restriction mapping of a 6.4 kb insert, a 2.2 kb dna fragment was sub-cloned in puc19 to produce the enzymically active clone pjw3. recloning of the gene fragment in the reverse orientation in puc18 (clone pjw4) indicated that a gene promoter was present in the cloned fragment and was able to function in e. coli. the clone pjw4 displayed increased ... | 1989 | 2628545 |
| cloning and expression in escherichia coli of a xylanase gene from bacteroides ruminicola 23. | a gene coding for xylanase activity in the ruminal bacterial strain 23, the type strain of bacteroides ruminicola, was cloned into escherichia coli jm83 by using plasmid puc18. ab. ruminicola 23 genomic library was prepared in e. coli by using bamhi-digested dna, and transformants were screened for xylanase activity on the basis of clearing areas around colonies grown on remazol brilliant blue r-xylan plates. six clones were identified as being xylanase positive, and all six contained the same 5 ... | 1989 | 2658806 |
| [sweet's syndrome]. | the authors describe a clinical case of sweet's syndrome in a 41-year-old man. from the clinical-instrumental tests carried out a paraneoplastic syndrome is excluded. | 1989 | 2698381 |