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should fiberoptic bronchoscopy aspirates be cultured?the reliability of fiberoptic bronchoscopy as a method to study the bacteriology of the lower respiratory tract was tested. the procedure used was suction aspiration through the inner channel after topical anesthesia with lidocaine. to detect contamination by oropharyngeal bacteria, the aspirates were cultured in patients with no evidence of active infection, comparison was made with results of transtracheal aspiration cultures, and the aspirate was tested for the presence of an oral dye marker. ...19767173
antibodies reactive with leptotrichia buccalis in human serum from infancy to adulthood. 19768029
influence of pencillinase production by strains of bacteroides melaninogenicus and bacteriodes oralis on pencillin therapy of an experimental mixed anaerobic infection in mice. 19768031
influence of amino acids on the growth of bacteroides melaninogenicus.addition of individual amino acids to a trypticase-yeast extract-hemin medium affected growth rates and final yields of an asaccharolytic strain and a saccharolytic strain of bacteroides melaninogenicus. l-aspartate or l-asparagine produced maximal growth enhancement for both strains. l-14caspartate was fermented by resting cells of the asaccharolytic strain. l-cysteine or l-serine also enhanced growth for the saccharolytic strain. however, growth of the saccharolytic strain was inhibited by l-l ...19768425
characterization of bacteroides melaninogenicus.fifty-eight human isolates of bacteroides melaninogenicus, 42 from a variety of clinical infections and the rest from normal flora, were studied for pigment production and ultraviolet light fluorescence and by forty biochemical and other tests, including end-product analysis by gas-liquid chromatography. in a number of instances, tests were repeated several times and the results were reproducible. agar plate dilution susceptibility tests were also performed to 12 antimicrobial agents. these 58 s ...197610317
bacterial interference by oropharynegeal and clinical isolates of anaerobic bacteria.anaerobic isolates were tested for bacterial inhibitory activity. of 144 isolates, 102 were from oropharynegeal washings, and 42 were from clinical specimens. thirteen facultative bacterial species (seven members of the enterobacteriaceae and six species of gram-positive cocci) were used as indicators of inhibition. eleven anaerobic species were isolated from oral secretions. all isolates of bacteroides melaninogenicus, the most commonly recovered species, consistently inhibited several species ...197610336
susceptibility of phosphomycin as a differential character for gram negative anaerobic bacilli. 197611203
evaluation of enrichment, storage, and age of blood agar medium in relation to its ability to support growth of anaerobic bacteria.by measuring the colony size of a variety of anaerobic bacteria isolated from clinical specimens, an evaluation was made of the benefits derived from the addition of several enrichments to blood agar medium commonly used for the growth of anaerobes. similar methods were used to study the effects of various storage conditions and age of the medium. the results were compared with those obtained on freshly prepared and enriched blood agar plates as well as commercially available blood agar plates. ...197611226
casamino acids enhance growth of bacteroides melaninogenicus.casamino acids enhance the growth of bacteroides melaninogenicus when added to various concentrations of trypticase. absence of a peptide, not amino acids, is responsible for the inability of casamino acids to support growth.197712141
a study of the pigment produced by bacteroides melaninogenicus. 197612196
septicemia caused by ristella. 197612929
fermentation of l-aspartate by a saccharolytic strain of bacteroides melaninogenicus.resting cells of bacteroides melaninogenicus fermented l-14caspartate as a single substrate. the 14c-labeled products included succinate, acetate, co2, oxaloacetate, formate, malate, glycine, alanine, and fumarate in the relative percentages 68, 15, 9.9, 2.7, 1.8, 1.0, 0.7, 0.5, and 0.06, respectively, based on the total counts per minute of the l-14caspartate fermented. ammonia was produced in high amounts, indicating that 96% of the l-aspartate fermented was deaminated. these data suggest that ...197713713
treatment of bacteroides endocarditis with carbenicillin.a heroin addict developed acute bacterial endocarditis with streptococcus viridans and bacteroides melaninogenicus. although blood cultures became negative during penicillin g and clindamycin therapy there was little clinical response. prompt clinical improvement was achieved with intravenous carbenicillin in a dose of 40 g daily. in vitro testing supported the superiority of carbenicillin therapy in this patient.197715455
the syndrome of inappropriate secretion of antidiuretic hormone associated with anaerobic thoracic empyema.the syndrome of inappropriate secretion of antidiuretic hormone has been associated with many pulmonary diseases, including tuberculosis and bacterial and viral pneumonia: however, it has not been reported with anaerobic infections or empyema in the absence of pneumonia. we report a patient with empyema due to bacteroides melaninogenicus, bacteroides oralis, and peptostreptococcus who developed the syndrome. eight hours before the start of therapy, his serum sodium concentration was 127 meq per ...197715491
purification and immunochemical characterization of the outer membrane complex of bacteroides melaninogenicus subspecies asaccharolyticus.morphologic study of bacteroides melaninogenicus subspecies asaccharolyticus by electron microscopy disclosed the presence of a capsule and a cell wall structure otherwise typical of a gram-negative organism. an outer membrane complex was isolated with use of gentle methods. relative purity of the preparation was confirmed by electron microscopy and by the formation of a single band in a sucrose density gradient. gel chromatography was used for separation of the major components of the membrane. ...197716065
penicillin resistance and penicillinase production in clinical isolates of bacteroides melaninogenicus.the minimum inhibitory concentrations (mic) of penicillin and six other antimicrobials were determined for 50 clinical isolates of bacteroides melaninogenicus. agar dilution susceptibilities were performed using supplemented brucella blood agar and the proposed national committee for clinical laboratory standards standard method for anaerobes; results with the two methods were comparable. a penicillin concentration >/=0.8 mug/ml was needed to inhibit 56% of the isolates, whereas 100% were suscep ...197716564
international aspects of oral microbiology.it appears that the bacteria of the mouth are remarkably similar throughout the world. there are differences, but they are mostly of a quantitative rather than a qualitative nature. although much is yet to be learned on the regulation of the oral ecosystems, these quantitative fluctuations to a large extent seem to be induced by different dietary patterns: the food exerting a strong selective pressure on the oral microbiota.197721852
sodium dodecyl sulphate-polyacrylamide gel electrophoresis of polypeptides from bacteroides melaninogenicus. 197722532
role of nucleosides, 5-phosphoribosyl-1-pyrophosphate, and ribose 1-phosphate in the biosynthesis of phosphosphingolipids in bacteroides melaninogenicus. 197823730
the hydrolysis of dextran by gram negative non-sporing anaerobic bacilli. 197724035
quantitative evaluation of three commercial blood culture media for growth of anaerobic organisms.the ability of three different commercial blood culture media--brain heart infusion broth (pfizer), thiol broth (difco), and prs-peptone broth (becton, dickinson & co.)--to support the growth of five different anaerobes is described. inocula of 100 and 1,000 colony-forming units per ml were used to evaluate potential differences in survival, lag time, growth rate, and doubling times of each anaerobe in each medium. in addition, each medium was evaluated for its ability to neutralize the antibact ...197824059
immunochemical and biologic studies of the lipopolysaccharide of bacteroides melaninogenicus subspecies asaccharolyticus. 197824073
clotting and fibrinolytic activities of bacteroides melaninogenicus.106 strains of bacteroides melaninogenicus ss. asaccharolyticus, intermedius and melaninogenicus were tested for production of extracellular blood clotting and fibrinolytic factors. 78.3% of tested strains caused clotting of rabbit plasma and 79.2% activated human plasminogen. strains producing the clotting or fibrinolytic factor only were isolated. both factors were quite active, as positive results for most strains were detected within one hour of incubation in controlled test system.197724963
the cellular hypersensitivity in guinea pigs immunized with bacteroides melaninogenicus through gingiva. 197626475
efficient fractionation of collagenolytic sample from oral bacteroides melaninogenicus. 197626476
bacteriocin-like activities of human dental plaque flora against oral anaerobic microorganisms. 197726477
a method for the isolation of bacteroides melaninogenicus from the human mouth.isolation procedures involving the direct plating of specimens and the use of selective, non-selective, transport and enrichment media were compared in respect of their value for the recovery of bacteroides melaninogenicus from the gingival sulcus. a selective medium containing kanamycin and vancomycin enhanced the recovery rate of b. melaninogenicus. vmgii transport medium was convenient to use and gave as good a recovery rate as that obtained with plates directly inoculated in the clinic.197826805
isolation and characterization of bacteroides melaninogenicus from human specimen (author's transl). 197827649
pleuropulmonary lung infection by anaerobic bacteria.two patients with severe pleuropulmonary infection caused by non-sporing anaerobic bacteria are described. one had an empyema with foul-smelling pus and developed bacteraemia, and the other developed a lung abscess. both were successfully treated with antibiotics and drainage, chest surgery being avoided. the successful diagnosis and treatment of these patients involved close liaison between clinical and laboratory staff.197829654
preparation of prereduced anaerobically sterilized media and their use in cultivation of anaerobic bacteria.several modifications of the roll-tube method have made it simpler for routine use in the isolation and growth of anaerobic bacteria. these include use of a check valve for the production of prereduced anaerobically sterilized media; a salvarsan tube under oxygen-free gas pressure for the dispensing of molten prereduced anaerobically sterilized agar medium; a kelly infusion bottle with a graduated pipette side arm (also under gas pressure) for quantitative delivery of fluid prereduced anaerobica ...197829909
[ecology of bacteroides melaninogenicus in the oral cavities of preschool children (author's transl)]. 197731099
growth inhibition of streptococcus mutans by the black pigment (haematin) of bacteroides melaninogenicus. 197831156
isolation of anaerobes in ludwig angina. 197931420
acute suppurative parotitis caused by anaerobic bacteria: report of two cases.staphylococcus aureus is the predominant pathogen in acute suppurative parotitis. although anaerobic bacteria are prevalent in the normal oral and upper respiratory tract flora and frequently are involved in oral and facial infections, they have seldom been isolated from patients with suppurative parotitis. it may be that a role for anaerobes in parotitis has not been documented because of inadequate specimen transport or anaerobic culture techniques. we describe two cases of acute suppurative p ...197832513
sphingolipid biosynthesis and vitamin k metabolism in bacteroides melaninogenicus.b. melaninogenicus provides a unique system for the study of the biosynthesis of an important group of lipids, the phosphosphingolipids. sphingolipid biosynthesis can be repressed and induced by depletion and restoration of vitamin k. at least one enzyme involved in sphingolipid biosynthesis from the microorganism can be solubilized and so purified by conventional methods. pathways involved in biosynthesis may differ from hitherto postulated pathways, for example, the incorporation of nh4+ into ...197932765
longitudinal study of experimentally induced periodontal disease in macaca arctoides: relationship between microflora and alveolar bone loss.macaca arctoides monkeys develop periodontal disease, and they harbor a periodontopathic indigenous flora largely similar to that of humans. this study showed that various haemophilus isolates and h2o2-splitting asaccharolytic bacteroides melaninogenicus strains constituted major segments of the monkey periodontal microflora. these organisms have not been previously identified among human isolates. furthermore, the present data revealed that asaccharolytic b. melaninogenicus strains increased in ...197933902
chromogenic cephalosporin spot test to detect beta-lactamase in clinically significant bacteria.lactamase production. reacteroides melaniongenicus, 14197934624
[phlegmon of the fore arm succeeding to a dog-bite : respective role of the miscellaneous bacteria involved in this mixed infection (author's transl)].the authors report a case of a severe mixed infection (phlegmon of the fore arm) succeeding to a dog bite. a complex flora has been isolated from this phlegmom : pasteurella multocida, pasteurella pneumotropica, streptococcus mitis, actinomyces sp., bacteroides melaninogenicus, and a gram- bacteria related to group ii j. the pathogeny of such diseases is discussed, dwelling in one hand on the part of synergistic development of these various germs and in the other hand on the part played by saliv ...197934886
[the bacterial flora of preputial space].the bacterial flora of the preputial space of 210 healthy males (43 children aged between 2 and 11 years, 137 males between 12 and 60 years and 30 men over 60 years) was determined by smears and cultueres from glans penis, sulcus coronarius and the adjacent prepuce. the results were grouped according to various criteria, e.g. glans covered or uncovered and age of males. differences in the distribution of germs could be found in relationship to age. in the case of an uncovered glans penis the pre ...197935486
antibiotic susceptibility of anaerobic bacteria with special reference to bacteroides fragilis.it was shown that recent swedish clinical isolates of anaerobic bacteria are susceptible to many antibiotics by the agar dilution method with the exception of the bacteroides group versus beta-lactam antibiotics or tetracyclines. strains of b. fragilis were inhibited by 4--greater than 128 micrograms benzylpenicillin or cephalothin/ml, 1.0--64 micrograms cefoxitin/ml, 0.064--2 micrograms clindamycin or metronidazole/ml, 2--8 micrograms chloramphenicol/ml, 2--16 micrograms fusidic acid/ml and 0.0 ...197937593
anaerobic pulmonary infections.the main cause of anaerobic pulmonary infections is aspiration of saliva, upper airway secretions or gastric content. predisposing conditions include prominent dental disease, chronic upper respiratory tract infections and reduced consciousness. fusobacterium nucleatum, bacteroides melaninogenicus and anaerobic gram-positive cocci are the most frequently encountered organisms. the clinical presentations are lung abscess, lung gangrene and empyema, which all tend to take a slow and indolent cours ...197937594
[in vitro ability of dental plaque formation by bacteroides melaninogenicus (author's transl)]. 197738768
the predominant cultivable microbiota of the periodontal abscess. 197938308
serological identification of bacteroides species by an enzyme-linked immunosorbent assay.an enzyme-linked immunosorbent assay was used to titrate antisera raised against live cultures of eight type species (biotypes) of bacteroides with the edta-released outer-membrane complex from 29 characterised strains of bacteroides species. with only minor exceptions, the strains investigated reacted to titre with the antisera raised against the homologous type species and not against the heterologous type species. cross-reactivity between heterologous species and antiserum was only significan ...197934633
complement activation by propionibacterium acnes and bacteroides melaninogenicus. 197833641
gas abscess transformation of a huge hypernephroma. 197941445
anaerobic isolates in chronic recurrent suppurative otitis media. treatment with carbenicillin alone and in combination with gentamicin.tympanocentesis was performed in 32 pediatric patients with chronic recurrent suppurative otitis media. the aspirate was cultured aerobically and anaerobically. aerobes were isolated from ten patients (31.2%); anaerobes from one patient; and both aerobes and anaerobes from 21 patients (65.6%). there were 46 aerobic isolates. the aerobes commonly recovered were pseudomonas aeruginosa (24 isolates) proteus sp. (5) and staphylococcus aureus (3). there were 32 anaerobes isolated including anaerobic ...197941811
fluorescent antibody test kit for rapid detection and identification of members of the bacteroides fragilis and bacteroides melaninogenicus groups in clinical specimens.the fluoretec fluorescent antibody test kit (pfizer inc., new york, n.y.), developed for the rapid detection of members of the bacteroides fragilis and b. melaninogenicus groups, was evaluated by testing 58 stock cultures and 76 clinical specimens. the test reagents detected 100% of 40 b. fragilis and b. thetaiotaomicron stock culture strains, although only 22% of 18 b. vulgatus, b. distasonis, and b. ovatus strains showed positive fluorescence. the 76 clinical specimens were evaluated by examin ...197941847
rapid diagnosis of anaerobic infections by gas-liquid chromatography.it was postulated that the short chain fatty acids (scfa) produced by anaerobic bacteria might serve as microbial markers in purulent material. eighteen pus specimens from various sources were analysed by gas-liquid chromatography (glc), and the scfa detected were compared with the microorganisms isolated by conventional methods. it was found that the detection of propionic, isobutyric, butyric, or isovaleric acids by direct glc of pus specimens is strong evidence for anaerobic infection but not ...197941850
metronidazole in the prophylaxis and treatment of anaerobic infection.the influence of prophylactic metronidazole on vaginal carriage rates of anaerobes and the development of postoperative anaerobic infection was studied in 104 women who underwent abdominal hysterectomy. metronidazole prophylaxis in 54 patients led to a decrease in the anaerobe vaginal carriage rate from 65% pre-operatively to 17% and 28% on the 3rd and 7th postoperative days respectively. in the control group (50 patients) no significant decrease in anaerobe yield was noted, corresponding percen ...197833454
survival of crevicular microorganisms in an artificial culture medium. 197943361
metronidazole in surgical infections. 197829029
beta-lactamases in bacteroides.bacteroides fragilis is responsible for most anaerobic infections in man. most isolates of b. fragilis show resistance to beta-lactam antibiotics. this resistance might be due to beta-lactamase production or permeability barrier in the cell wall. b. fragilis produce beta-lactamase with mainly cephalosporinase activity. other bacteroides species such as b. clostridiformis, b. melaninogenicus and b. oralis also produce beta-lactamase but with different biochemical characteristics.197828561
[are periodontal diseases specific infectious diseases?]. 197844349
an experimental model for osteomyelitis in the mandible. 197728338
black-pigmented bacteroides from clinically characterized periodontal sites. 197944523
early diagnosis of crepitant gangrene caused by bacteroides melaninogenicus.a rapid non-invasive test for the presence of b. melaninogenicus in the wounds of crepitant non-clostridial gangrene is described. the wounds are viewed under an ultraviolet light, and the presence of bright red fluorescene indicates the probable presence of b. melaninogenicus.197827832
the role of bacteroides melaninogenicus and other anaerobes in periodontal infections.recent taxonomic and anatomical studies of dental plaque associated with periodontal health and disease have demonstrated that differences in the microbial populations in plaque may be responsible for the initiation and progression of disease. the consistent isolation of large numbers of anaerobic and capnophilic bacteria from the depths of periodontal lesions has suggested an important role for these organisms. bacteria that have been isolated include capnocytophaga (bacteroides ochraceus), oth ...197944923
virulence factors of anaerobic bacteria: summary of the symposium. 197944924
the nature of clotting and fibrinolytic activities of bacteroids melaninogenicus.from the supernatant of b. melaninogenicus ss. asaccharolyticus culture, a protein fraction was isolated by ethanol precipitation. the fraction was tested for the presence of clotting and fibrinolytic activities by application of quantitative techniques and specific substrates for measurement of prothrombin and plasminogen activation, and collagenase and elastase activity. it is postulated that ability of bacteroides melaninogenicus ss. asaccharolyticus extracellular factors to clot fibrinogen a ...197824965
attachment of bacteroides melaninogenicus subsp. asaccharolyticus to oral surfaces and its possible role in colonization of the mouth and of periodontal pockets.this investigation examined the ability of cells of bacteroides melaninogenicus subsp. asaccharolyticus 381 to adhere to surfaces that might be important for its initial colonization of the mouth and its subsequent colonization in periodontal pockets. of 48 asaccharolytic strains of b. melaninogenicus, 47 agglutinated human erythrocytes, whereas none of 20 fermentative strains, which included reference cultures of the subspecies intermedius and melaninogenicus, were active. electron microscopy i ...197824002
inhibition of phagocytosis in vitro by obligate anaerobes.a variety of species of obligate anaerobes have been shown to interfere with the phagocytosis and killing of proteus mirabilis and other aerobic bacteria in vitro. although all the obligate anaerobes examined showed this activity, the effect was greatest with strains of bacteroides melaninogenicus and b. fragilis. in contrast, none of the 36 aerobes tested acted in this way. these observations suggest that the presence of obligate anaerobes may be fundamental to the pathogenesis of some types of ...197773952
severe arm pain associated with pathological bone cavity of maxilla. 197874557
abscesses of the frontal lobe of the brain secondary to covert dental sepsis.the bacterial species found in pus aspirated from brain abscesses in two patients were typical of those found in dental sepsis. subsequently apical-root abscesses were demonstrated in the upper jaws of both patients. this evidence strongly suggests that these cerebral abscesses were secondary to dental sepsis which could have spread from the teeth to the frontal lobes by several possible antaomical pathways.197879867
chemotherapy of brain abscess. 197882092
local antibody production in guinea pigs by gingival inoculation of bacteroides melaninogenicus. 197523908
anaerobic breast abscesses. 197984192
chemical composition, serological reactivity and endotoxicity of lipopolysaccharides extracted in different ways from bacteroides fragilis, bacteroides melaninogenicus and bacteroides oralis.lipopolysaccharides (lps) extracted from strains of bacteroides fragilis, bacteroides melaninogenicus and bacteroides oralis with phenol-water, trichloroacetic acid, edta or liquid phenol-chloroform-petroleum ether (pcp) and isolated by ultracentrifugation, varied considerably in their quantitative chemical composition. negligible yields of lps were obtained by pcp-extraction. all preparations were more or less serologically active. all methods (except pcp) extracted the same o-antigenic determi ...197719922
[capsular-like structure in b. melaninogenicus as revealed by gins' negative staining method (author's transl)]. 197893958
survival of anaerobic and aerobic bacteria on cotton swabs in three transport systems.the capacity of aerobic and anaerobic bacteria to survive on cotton swabs placed into a dry gassed-out co2-filled tube (dgt), dry sterile aerobic tube (dat), and a tube containing a modified stuarts' transport medium (mst), was assessed. pseudomonas aeruginosa increased in numbers by 2 and 3 logs when stored in mst and dat, respectively. the viability of p. aeruginosa, although retarded when compared to msa and dat, was not adversely affected by the co2 environment in the dgt. the mst maintaine ...1975240870
recovery and clearance rates of oral microorganisms following experimental bacteraemias in dogs. 1975242303
[morphological study of several vegetative and reproductive cycles of prevotella bacteriogloeae novum genus, nova species].a new bacteria named prevotella bacterioglaeae is studied in curious types of fructification. its history over the period of a year is given in relation with conditions of temperature. this bacteria is placed in the other of chamydobacteriales.1976821637
the classification of bacteroides melaninogenicus and related species. 197717592
microflora in the healthy gingival sulcus in man.the roll tube culture technique was utilized to examine quantitatively and qualitatively the predominant cultivable microflora inhabiting the clinically healthy gingival sulcus. seven periodontists aged 32-54 years were included in the study. from a total of 350 isolates, 85 (24.3%) were obligate anaerobes, 53 (15.0%) were gram negative, and 197 (56.3%) were categorized as rods. actinomycetes predominated in three samples. four samples were dominated by streptococcus species. the flora as reveal ...197717152
energy-dependent incorporation of sphingolipid precursors and fatty acids in bacteriodes melaninogenicus.washed cells of bacteroides melaninogenicus are unable to incorporate the sphingolipid precursor 3-ketodihydrosphingosine (3kds) or dihydrosphingosine into the complete sphingolipids ceramide phosphorylethanolamine (cpe) and ceramide phosphorylglycerol (cpg), whereas growing cultures are able to do so. this result suggested that an energy source was required by washed cells to initiate the incorporation of 3kds. investigation of a number of energy sources for b. melaninogenicus showed that gluta ...197715984
comparison of the biochemical properties of bacteroides melaninogenicus from human dental plaque and other sites. 197614095
gingival crevicular fluid concentration and side effects of minocycline: a comparison of two dose regimens.the purpose of this study was to conduct a direct comparison of two dose regimens of minocycline to determine 1) whether they achieved crevicular fluid concentrations in a therapeutic range; and 2) the frequency of side effects. in a double-blind design, 30 patients divided into 2 groups were given either 100 mg minocycline or 200 mg minocycline per day for an 8-day period. the concentration of minocycline in the gingival clevicular fluid (gcf) at 8 days was 4.77 micrograms/ml for the 100 mg a d ...19921313100
beta-lactamase activity in strains of bacteroides melaninogenicus and bacteroides oralis.beta-lactamase from strains of bacteroides melaninogenicus and bacteroides oralis hydrolyzed penicillin more rapidly than ampicillin or carbenicillin. cephalothin and a chromogenic cephalosporin (87/312) were also hydrolyzed by the enzyme. activity was found only in beta-lactam-resistant strains, but there was considerable variation in activity among strains having the same minimal inhibitory concentrations of antibiotic. beta-lactamase activity was cell bound and appeared to be tightly associat ...197713705
leukocyte migration inhibition by bacterial antigens in patients with periodontal disease. 197712257
an unusual bilateral pathological fracture.an unusual case of a bilateral pathological fracture of the mandible has been presented. the fracture of the right side of the mandible was through an area of bacteroides osteomyelitis and the fracture of the left side of the mandible was through a large cementifying fibroma, which had replaced the left body of the mandible.197711280
blastogenic response of human lymphocytes to oral bacterial antigens: comparison of individuals with periodontal disease to normal and edentulous subjects.cell-mediated immunity in humans to antigens derived from oral plaque bacteria was investigated by using the lymphocyte blastogenesis assay. subjects with varying severities of periodontal disease including normal, gingivitis, periodontitis, and edentulous were compared. mononuclear leukocytes were separated from peripheral blood and cultured with antigens prepared by sonication of actinomyces viscosus (av), actinomyces naeslundii (an), veillonella alcalescens (va), leptotrichia buccalis (lb), b ...197610259
gene cloning of porphyromonas gingivalis specific antigens recognized by serum of adult periodontitis patient.1. porphyromonas gingivalis is believed an important pathogen of adult periodontitis. a gene library of p. gingivalis 381 was constructed in lambda phage vector l47.1. the library was probed with serum obtained from patients of severe adult periodontitis. two clones, lambda mdbg101 and lambda mdbg103 which were expressed, 200 and 160 kda respectively, were selected and further studied. 2. the expressed antigens in these two clones were also reacted with rabbit antiserum against whole cells, caps ...19921319357
e-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms.the purpose of the study was to validate the epsilometer test (e-test) method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard. the e-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. the device consists of a predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip. after 48 hours incub ...19921324301
development of a rapid latex agglutination test for periodontal pathogens.the studies reported here describe the development, characterization, and initial application of latex agglutination assays for periodontal pathogens. latex reagents were prepared by sensitization of latex microspheres with rabbit igg antibodies to either actinobacillus actinomycetemcomitans, porphyromonas gingivalis, or prevotella intermedia. the protein concentration utilized for sensitization and microsphere size were optimized. one reagent was prepared to a. actinomycetemcomitans and a secon ...19921324302
description of a polyvalent conjugate and a new serogroup of bacteroides melaninogenicus by fluorescent antibody staining.a polyvalent conjugate (fluorescein isothiocyanate-labeled antibody reagent) containing serogroups a, b, and c conjugates was prepared. this polyvalent conjugate gave a positive fluorescent antibody (fa) stain with 49 stains of bacteroides melaninogenicus representing serogroups a, b, and c. when additional strains (92 strains) of the three subspecies of b. melaninogenicus were examined by the fa stain, with a, b, and c, and polyvalent conjugates, nine strains of b. melaninogenicus subsp. interm ...19766487
the polysaccharide capsule of bacteroides fragilis subspecies fragilis: immunochemical and morphologic definition.a large-molecular-weight capsular polysaccharide was isolated from strains of bacteroides fragilis subspecies fragilis. by means of electron microscopy and staining with ruthenium red, the thick polysaccharide capsule was also visualized. with use of a radioactive antigen-binding assay, antibody to this capsular polysaccharide was demonstrated in antisera prepared in rabbits to each of eight strains of b. fragilis fragilis. antibody of similar specificity was not found in antisera prepared to ba ...19761451
rapid screening of veillonella by ultraviolet fluorescence.among 51 strains of anaerobic gram-negative cocci belonging to the family veillonellaceae, all strains of veillonella (v. parvula and v. alcalescens) displayed red fluorescence under long-wave (366 nm) ultraviolet light, whereas no acidaminococcus or megasphaera demonstrated fluorescence. in contrast to bacteroides melaninogenicus, growth of veillonella does not require hemin and menadione, and flourescence is rapidly lost upon exposure to air. the fluorescent component of a strain of v. parvula ...19751419
[anaerobic gram negative septicemias in digestive tract surgery and in surgical intensive care]. 197939472
improved procedure for transport of dental plaque samples and other clinical specimens containing anaerobic bacteria.an improved transport system for samples containing anaerobic bacteria was developed. this system increased the recovery rate of anaerobic bacteria up to 28.8% as compared to a commonly used method.197939087
the porphyrin pigmentation of subspecies of bacteroides melaninogenicus.various subspecies of bacteroides melaninogenicus differ in their pigmentation. subsp. asaccharolyticus produces protohaem almost exclusively, subsp. intermedicus both protohaem and a smaller proportion of protoporphyrin, and subsp. melaninogenicus mainly protoporphyrin with a trace of protohaem. as a consequence young colonies can be differentiated by their red fluorescence in u.v. light (365nm): subsp. asaccharolyticus does not fluoresce, subsp. intermedicus shows a limited fluorescence, and s ...197939545
characterization of anaerobic gram-negative bacilli by using rapid slide tests for beta-lactamase production.a total of 175 isolates of anaerobic gram-negative bacilli were tested for beta-lactamase production by using a slide test modification of the chromogenic cephalosporin (nitrocefin, glaxo, middlesex, england) assay and the iodometric slide test. included isolates were bacteroides melaninogenicus (46), b. fragilis (78), other bacteroides isolates (21), fusobacterium (25), and other gram-negative bacilli (5). both slide tests detected 25 b. melaninogenicus isolates that were beta-lactamase produce ...197941001
clinical and microbiological aspects of chemotherapeutic agents used according to the specific plaque hypothesis.certain forms of dental decay and periodontal disease appear to be due to specific bacterial infections following overgrowth of certain indigenous plaque bacteria, i.e., the specific plaque hypothesis, if so, then antimicrobial treatment based on a diagnosis of elevated levels or proportions of these organisms should be considered. such treatment cannot be administered according to concepts of the non-specific plaque hypothesis. a treatment philosophy is presented which is based upon considerati ...197941862
further studies on some physical and biochemical characteristics of asaccharolytic pigmented bacteroides of feline origin.the ultrastructure of the appendages of 24 strains of asaccharolytic pigmented bacteroides spp. of cats was studied by transmission electron microscopy. all strains examined by thin section showed abundant fimbriae, outer membrane vesicles and capsules. negative staining showed fimbriae which varied from long, fine and wavy in bact. salivosus and cat group 2 to shorter, less abundant and thicker fimbriae in cat strains of bact. gingivalis as well as type strains of porphyromonas gingivalis and p ...19921353758
detection of anticapsular antibodies to bacteroides asaccharolyticus in serum from rabbits and humans by use of an enzyme-linked immunosorbent assay.a sensitive serologic test, the enzyme-linked immunosorbent assay (elisa), was used to detect serum igg antibodies directed specifically to a capsular antigen of bacteroides asaccharolyticus (previously known as bacteroides melaninogenicus subspecies asaccharolyticus). anticapsular igg was measured in 30 specimens of rabbit serum after the animals were immunized with whole b. asaccharolyticus, the two subspecies of b. melaninogenicus, and several other bacterial species. species-specific anticap ...197944309
comparative ultrastructure of bacteroides melaninogenicus subspecies. 197944319
the influence of environment on the oral flora of shangaan mineworkers. 197844502
immunochemical characterization of surface antigens of bacteroides melaninogenicus.major surface antigens of bactmbrane complex by gentle methods, purified, and characterized immunochemically. a lipopolysaccharide (lps) was found to be chemically distinct from the lps of facultative gram-negative bacteria in that it lacked two core sugars, 2-keto-3-deoxyoctonate and heptose, as well as beta-hydroxymyristic acid, the predominant fatty acid in the lipid a moiety. the lps was further atypical in that it had a very low level of biologic activity. a capsular polysaccharide was demo ...197944922
the management of acute, serous and chronic otitis media: the role of anaerobic bacteria.otitis media (om) is a common childhood disease and one which can cause significant morbidity. a knowledge of the pathogens responsible for om enables the most appropriate treatment regimen to be selected and thus minimizes further complications which may require hospital admission and surgery. the microbiology of acute, serous and chronic om is reviewed, with particular regard to the role of anaerobic bacteria. anaerobes, mainly gram-positive cocci, have been recovered from 25% of the ear aspir ...19921362753
genetic relationship between different subspecies of bacteroides melaninogenicus. 197945296
microbiologic and pathologic aspects of pulpal and periapical disease.the greatest cause of endodontic and periapical pathosis is microbial infection of the pulp. most odontogenic infections are of a polymicrobial nature. with advances in anaerobic isolation and culturing techniques, much has been learned about the presence of pathogenic organisms such as porphyromonas and prevotella species (formerly classified as black-pigmented bacteroides species) in infected root canals. this review provides a summary of recent developments in endodontic microbiology, virulen ...19921381632
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