hormonal control of zinc uptake and binding in the rat dorsolateral prostate.the zinc uptake in the dorsolateral prostate of rats was studied after different hormonal manipulations. orchiectomy reduced the uptake of 65zn. administration of estradiol benzoate to orchiectomized rats doubled the 65zn uptake, a phenomenon which was not observed in orchiectomized-adrenalectomized rats. adrenalectomy in orchiectomized rats had no effect on the concentration of radioactivity beyond the castration-induced decrease. a prolactin release inhibitor, 6-methyl-8-erogelenylacetamide, r ...197715955
cell-mediated immune response in mice treated with steroidal contraceptives.the splenomegaly assay (simonsen, 1962) was standardized using different strains of rats and mice. male wistar rat (donor)-female swiss mouse (host) was found to be the suitable combination that could be employed in subsequent experiments to study the potential of contraceptive steroids to alter cmir. the index of splenomegaly appeared to increase in case of mice treated with combination oral contraceptives (ovulen, ovral or enovid). the differences observed, however, neared significance only in ...197555327
a serial study of pregnancy proteins in primigravidae.the plasma concentrations from four 'pregnancy proteins' and three steroid hormones have been measured throughout pregnancy in 15 primigravidae. two of the proteins, human placental lactogen (hpl) and pregnancy-specific beta1-glycoprotein (psbetag), are specific for pregnancy and correlate well with the stage of gestation. it is suggested that measurement of psbetag may be useful in assessing feto-placental wellbeing. neither of the pregnancy-associated proteins, steriod-binding beta-globulin (s ...197657799
serological definition of the lentivirus group of retroviruses.the major polypeptides of visna viruses and other lentiviruses have been isolated and shown to be closely related if not identical in radioimmunoassays. by this criterion the lentiviruses form a distinct group of retroviruses unrelated to spuma viruses, mammalian and avian retroviruses that cause tumors, and unclassified retroviruses of cattle and horses. two sera obtained from goats immunized with mason-pfizer monkey virus or squirrel monkey virus reacted with visna p30. additional data suggest ...197985722
the isolated human cortex. a golgi analysis of krabbe's disease.the clinical course of a child with krabbe's leukodystrophy was characterized by clinical seizures, startle myoclonus, and paroxysmal activity recorded by eeg. at autopsy in the fourth year, myelinated subcortical axons were destroyed, virtually completely. despite isolation from major subcortical and interhemispheric connections, the cell and fiber pattern of the cortex appeared remarkably normal in routine histologic preparations. the normal range of pyramidal and stellate interneurons were al ...197986346
chronotropic response of isolated atria to acid base alterations.the effect of acid-base alterations on spontaneous rate was analysed using isolated atria exposed to cumulative degrees of acidosis produced either by adding hcl or by increasing pco2 in the incubation medium. frequncy vs. ph curves were made to assess chronotropic response to acid-base changes. heart rate was increased in alkalosis and decreased when the ph of the medium was lowered. both "respiratory" and "metabolic" alterations affected the contraction rate to the same extent. decreasing ph f ...197887182
an immunogenetic analysis of the mhc of the rat--agb(rt1). i. serologic data. 197989739
distribution of myosin isoenzymes among skeletal muscle fiber types.using an immunocytochemical approach, we have demonstrated a preferential distribution of myosin isoenzymes with respect to the pattern of fiber types in skeletal muscles of the rat. in an earlier study, we had shown that fluorescein-labeled antibody against "white" myosin from the chicken pectoralis stained all the white, intermediate and about half the red fibers of the rat diaphragm, a fast-twitch muscle (gauthier and lowey, 1977). we have now extended this study to include antibodies prepare ...197990047
effects of arachidonic acid and its metabolites on antigen-induced histamine release from human basophils in vitro. 197990097
polyribonucleotide inhibition of ribonucleic acid directed deoxyribonucleic acid polymerase of mouse mammary tumor (type b) virus and simian sarcoma (type c) virus. 197990331
characterization of a type-c virus produced by co-cultures of human leukemic bone-marrow and fetal canine thymus cells.the putative human helper virus ska-21/a204v, isolated by nooter et al. in 1977 from human leukemic bone-marrow cells following co-culture with normal fetal canine thymus cells, cf2th, has been characterized with respect to its major viral core protein, reverse transcriptase, and nucleic acid sequences. the results of these analyses show that this virus is not distinguishable from the woolly monkey type-c virus, ssav-1, by the techniques employed.197990662
antigen-specific suppressor factors produced by t cell hybridomas for delayed-type hypersensitivity.this study describes the generation of t hybridoma lines which secret factors specifically suppressing delayed-type hypersensitivity (dth) to sheep red blood cells (srbc). akr strain-derived t lymphoma bw 5147 cells were fused with spleen cells from mice primed with srbc and containing antigen-specific t suppressor cells for dth. supernants from the derived hybridomas were tested for suppression of either expression of induction of dth to srbc. six lines produced specific suppressor activity for ...197991526
shielding of phospholipid vesicles from phospholipase c hydrolysis by alpha-lactalbumin adsorption [proceedings]. 197992261
phylogeny of the rabbit gamma-chain determinants: a d12-like antigenic determinant in pronolagus rupestris.a survey for constant region gamma-chain allotypes (de locus) was undertaken in different lagomorph genera. as yet only pronolagus rupestris, a paleolaginae, showed the presence of a determinant similar to rabbit d12 although it lacked the widespread e15 determinant. all seven individuals possessed the d12 like determinant which was studied by immunodiffusion, haemagglutination inhibition, radiobinding and binding inhibition assays. in addition, a new enzymic method for typing for d12, based on ...197993133
a novel class of schizosaccharomyces pombe mutants, phenotypically unable to grow on glycerol, a respiratory substrate, but still able to oxidize glycerol aerobically [proceedings]. 197993469
a comparative study in porcellio laevis (lat.) and armadillidium vulgare (lat.): response to temperature, photoperiod and oxygen concentration.relationship of body weight, imposed fasting, temperature, light intensity, and oxygen concentration to oxygen consumption in porcellio laevis and armadillidium vulgare has been investigated in a series of laboratory experiments. it was observed that (1) the metabolic response in the two species to temperature change was a uniform increase of oxygen consumption with increasing temperature from 15 degrees c to 60 degrees c. beyond 30 degrees c, the oxygen consumption in each species fell, and the ...197993887
study on the significance of bronchial hyperreactivity in the bronchus obstruction after inhalation of cat dander allergen.the role of bronchial hyperreactivity in the process that leads to bronchial obstruction after inhalation of an allergen was investigated. in 30 asthmatic children selected because of a positive skin test to cat dander allergen, we measured the histamine threshold, the reaction after allergen inhalation, the allergen-specific ige concentration in serum, the lowest allergen concentration to which the intracutaneous skin test was positive (skin titer), and the histamine release of leukocytes after ...197992487
procaine-induced seizures in epileptic monkeys with bilateral hippocampal foci.intravenous procaine hcl given at low doses (0.5-2.5 mg/kg) to two monkeys with bilateral alumina hippocampal foci depressed interictal spiking or had little effect. at 5.0 mg/kg unilateral limbic activation occurred. at 10.0 mg/kg unilateral or bilateral limbic activation and generalized seizures could be evoked within 3-10 min. at higher doses (15 and 20 mg/kg) bilateral limbic activation or brief (one min) generalized seizures occurred. the unilateral-onset psychomotor seizures were not ident ...197991502
a 16-fold semi-microelectrode for intracortical recording of field potentials.a 16-fold semi-microelectrode with 10 x 10 sq. microns contacts at distances of 150 microns, produced by means of thin-film technology, is described. these small dimensions are required to study the laminar electrophysiological differences within the cerebral cortex.197991493
adequacy of breast-feeding.there are 3 main errors in the paper by professor waterlow and dr. thomson on the subject of adequacy of breast feeding. the errors relate to the following: validity of recommended dietary allowances (rda), balance between intake and requirements, and frequency of weight faltering. far from being "generally accepted," the rdas for early infancy (0-6 months) are highly tenuous. as waterlow and thomson indicate, they are largely based on s.j. fomon's studies on intakes observed in north americ ...197990775
cea-like antigens in different histologic types of human lung carcinoma.comparative studies on the presence and immunological reactivity of cea and cea-like antigens in lung and digestive tract cancers were performed. specific goat and rabbit anti-cea immune sera were used. immunofluorescence studies revealed in histologically different types of the lung carcinomas the presence of the cross reacting antigen with classical cea. absorption procedures, and double diffusion tests have shown the existence of two at least antigenic determinants in cea molecule: 1. the det ...197989630
[post-extrasystolic and post-pacing potentiation. ii. study by means of systolic time intervals in man]. 197888948
heterogeneity of constitutive heterochromatin in somatic syrian hamster chromosomes.syrian hamster constitutive heterochromatin was analyzed for c-band distribution and for bru late-replication pattern. characteristic for this species is relatively large amounts of sex-chromosome and autosomal heterochromatin. the distribution of constitutive heterochromatin was determined. the long term of the x chromosome, the whole y, the short arms of 8 autosomal pairs, the long arm of the smallest metacentric pair, and the centromeric regions of 12 pairs stained intensely dark on c-band pr ...197988308
interaction of diphosphatidylglycerol with beef heart cytochrome c oxidase in reconstitution experiments [proceedings]. 197994792
human breast carcinoma antigen is immunologically related to the polypeptide of the group-specific glycoprotein of mouse mammary tumor virus.we have shown [mesa-tejada, r., keydar, i., ramanarayanan, m., ohno, t., fenoglio, c. & spiegelman, s. (1978) proc. natl. acad. sci. usa 75, 1529--1533] that an antigen immunologically related to gp52, a 52,000-dalton glycoprotein of the mouse mammary tumor virus, can be identified in sections of human breast cancer by means of an indirect immunoperoxidase technique. the specificity of the reaction was established by absorption experiments which revealed that only purified gp52, or material cont ...197988056
[immunosuppressive activity of variously prepared alpha-globulin fractions of human blood]. 197994844
ability of enriched immune t cells to confer resistance in hamsters to infection with treponema pertenue.this investigation presents the first direct evidence that t cells are involved in resistance to challenge with treponema pertenue. enriched t cells from immune hamsters were obtained by sequential filtration through glass and nylon-wool columns. this procedure removed the majority of functional antibody-producing and immunoglobulin-bearing cells. the fractionated cell suspensions were less responsive to stimulation by phytohemagglutinin, lipopolysaccharide, and dextran sulfate, but they were en ...197994906
increased ratio of 5 alpha-reductase: 3 alpha (beta)-hydroxysteroid dehydrogenase activities in the hyperplastic human prostate.the activities of 5 alpha-reductase and 3 alpha (beta)-hydroxysteroid dehydrogenase were assayed in homogenates of eight normal, 21 hyperplastic and four carcinomatous human prostates. samples consisting of 300--500 microgram tissue protein in tris buffer, ph 7.0, were incubated at 37 degrees c for 30 min in the presence of 50 nm-[3h]androgen and an nadph-generating system started with 5 x 10(-4)m-nadp. the yield of 5 alpha- and 3 alpha-reduced metabolites, as established by using t.l.c. and g.l ...197986594
spontaneous termination of reentry ventricular tachycardia in the late myocardial infarction period: an experimental study in the dog.forty episodes of induced ventricular tachycardia in the late myocardial infarction period (4-6 days old) were analyzed in 12 dogs in an attempt to identify the possible mechanisms for the termination of reentry tachycardia. multiple epicardial and endocardial composite electrograms were recorded in and around the central ischemic zone of the infarction. during tachycardia, the earliest site of activation was identified in the epicardial surface of the border or normal zone immediately adjacent ...197995291
immunochemical characterization of the plasmin-antiplasmin system. basis for the specific detection of the plasmin-antiplasmin complex by latex agglutination assays. 197985679
molybdenum independence of nitrogenase component synthesis in the non-heterocystous cyanobacterium plectonema.the cyanobacterium plectonema boryanum (iu 594-utex 594) fixes n2 only in the absence of combined n and of o2. we induced nitrogenase by transfer to anaerobic n-free medium and studied the effect of mo starvation on nitrogenase activity and synthesis. activity was first detected within 3 h after transfer by the acetylene reduction assay in controls, increasing for at least 25 h. cells grown on nitrate and mo and then transferred to n-free, mo-free medium produced 8% of the control nitrogenase ac ...197896092
pattern of phenazine pigment production by a strain of pseudomonas atypical strain of pseudomonas aeruginosa capable of synthesizing three phenazine pigments was isolated. cultural conditions, under which the strain forms either chlororaphin, oxychlororaphin, or pyocyanine, are described. this broad spectrum of pigment production, as well as some other characteristics, sets this strain apart from previously described chlororaphin producers.197896094
pattern of activity of nucleolus organizers during spermatogenesis in mammals as analyzed by silver-staining.silver-staining in the nuclei and chromosomes of spermatogenesis of four species of mammals (man, mus musculus, rattus norvegicus, and cavia cobaya) was investigated qualitatively and quantitatively. these species show a very similar pattern of activity of the nucleolus organizer regions (nors) during the various stages of spermatogenesis. silver precipitates are detectable in growing spermatogonia and up until the pachytene stage of meiotic prophase. during the meiotic metaphases i and ii and d ...197985513
food-borne diseases: a food service supervisor's dilemma. 197896375
solid phase indirect radioimmunoassays for the rapid diagnosis of sindbis virus antigen.indirect radioimmunoassays have been developed for the rapid detection of sindbis virus. dilutions of sindbis virus from tissue culture fluids have been immobilized and allowed to react with rabbit anti-sindbis virus antibodies. the bound antibodies were assayed either by 125i-labelled anti-rabbit igg-antibodies or alternatively by addition of human complement and 125i-labelled anti-human c1q antibodies or 125i-labelled protein a.197884855
localization of nors in chromosomes of mouse cell lines by a combined 33258-hoechst and ag-staining technique. 197984805
the allergenic structure of allergen m from cod. iii. studies on the antigenicity of long-sequence peptides.fragments tm 1 (75 amino acid residues) and tm 2 (38 amino acid residues), and 3 other polypeptides (range 16-25 amino acid residues) of allergen m from cod were shown to be active in rabbit igg-mediated reactions. the same peptides were previously found to possess reactivity in ige-mediated reactions, thus suggesting a structural relationship between their antigenic and allergenic determinants.197984803
multivalvular vegetations in a patient with enterococcal endocarditis diagnosed by echocardiography.a patient with enterococcal endocarditis of 11 months' duration is presented, and the role of surgery and echocardiography is reviewed. echocardiography revealed vegetations of the aortic and mitral valves. after appropriate antibiotic therapy the patient had successful aortic and mitral valve replacement with porcine heterografts. enterococcal endocarditis is increasing in frequency and is likely to infect young women of childbearing age, elderly men who have had genitourinary tract manipulatio ...197896535
cross-reaction between the mycelial galactomannas of three hormodendrum strains and the mannans of two candida albicans strains of different serotypes, a and b.cross-reactivity between galactomannans of three representative hormodendrum strains, h. pedrosoi ifo 6071, h. compactum ifo 6726, and h. dermatitidis ifo 6421, and the mannans of two candida strains, c. albicans nih a-207 (serotype a) and c. albicans nih b-792 (serotype b), were investigated by means of agar-gel double diffusion, quantitative preciptin reaction, and passive cutaneous anaphylaxis. the following results were obtained. (i) antisera to whole cells of three hormodendrum strains were ...197984792
the fate of bacteriophage phie transfecting dna. 197896585
polarized light retinal photography of a monkey eye. 197896590
fluorescence and electron microscope studies on the interaction between lysosomes of mammalian host-cells and toxoplasma gondii rh following treatment with cotrimoxazole [proceedings]. 197884590
equine markers genes. polymorphism for group-specific component (gc).polymorphism of equine gc protein was demonstrated by immunofixation electrophoresis with a goat anti-human gc antibody. three different phenotypes, f, fs and s, were found. family data supported the genetic theory of two autosomal codominant alleles, gcf and gcs. both alleles occurred in standardbred, thoroughbred and arabian horses and in shetland ponies. a frequency of 0.23 for gcs in the american standardbred horse indicates the system should be useful for problems of identification and pare ...197884540
retrograde axonal transport of antibody to dopamine-beta-hydroxylase. 197656975
the ph-dependence of the non-specific esterase activity of carboxypeptidase a.the hydrolysis of the following 6 esters by bovine pancreatic carboxypeptidase a (peptidyl-l-amino-acid hydrolase, ec has been investigated over the range ph 5--10 at 25 degrees c, ionic strength 0.2: ch3co2chrco2h (r = c6h5 (ester 3), c6h5ch2 (ester 4), 4-no2c6h4co2chrco2h (r = c6h5 (ester 5), c6h5ch2 (ester 6), ch3(ch2)2 (ester 7)), ch3ch2co2ch(ch2c6h5)-co2h (ester 9). for each ester the ph dependence of kcat/km indicates that substrate binding is controlled by an acid of pkeh = 9.2 ...197831187
the effects of acridine orange on deoxyribonucleic acid in escherichia coli.1. acridine orange inhibits growth of escherichia coli k12 when incubated at ph 7.9, but not at ph 7.4.2. at a non-permissive temperature for dna polymerase i, acridine orange inhibits growth of a temperature-sensitive strain and also increases the rate of elimination of the f'-lac plasmid. 3. dna isolated from cells treated with acridine orange under conditions that inhibit growth contains material of low molecular weight, which is absent from dna isolated from cells treated under conditions in ...197827167
[interaction of epidermal growth factor with human fibroblasts in culture: an autoradiographic study at the ultrastructural level].the potent mitogen epidermal growth factor (egf) binds to specific receptors on human fibroblasts. in the present study we have used a quantitative em autoradiographic approach to visualize the events involved in the binding process. when 125i-egf is incubated at 4 degrees c for 120 min, labeled egf primarily localizes to the plasma membrane of the fibroblast but when incubated at 37 degrees c for 120 min., over 2/3 of the labeled material is internalized by the cell. the internalized radioacit ...197897010
characterization of receptors mediating 5-hydroxytryptamine- and catecholamine-induced platelet aggregation, assessed by the actions of alpha- and beta-blockers, butyrophenones, 5-ht antagonists and chlorpromazine.1 blood platelets from normal human volunteers were isolated and aggregated in vitro with 5-hydroxytryptamine (5-ht), noradrenaline (na), dopamine and n-dimethyldopamine (dmda). 2 the effects of 5-ht antagonists, alpha- and beta-adrenoceptor blocking agents, butyrophenones and chlorpromazine upon aggregation induced by the four amines were investigated. 3 only the the 5-ht antagonists, chlorpromazine and spiroperidol were potent inhibitors of 5-ht-induced aggregation, and only phentolamine was a ...197826445
effect of carotid artery ligation on regional cerebral blood flow in normotensive and spontaneously hypertensive rats.regional cerebral blood flow (rcbf) was measured in normotensive rate (ntr) and spontaneously hypertensive rats (shr), in a lightly anesthetized state and with control of paco2 by artificial ventilation. without carotid artery ligation, ntr and shr showed almost identical rcbf values and distribution, despite significantly elevated levels of blood pressure in shr. bilateral carotid artery ligation, however, caused much more pronounced decreases of rcbf (ischemia) in shr than ntr, in regions supp ...197716364
immunologic regulation in pregnancy.theories to explain the failure of rejection of the fetus by the mother during pregnancy include immunologic privilege of the uterus as a graft site, lack of transplantation antigen expression on the trophoblast, weakening of maternal cellular immunity during pregnancy, and separation of maternal and fetal circulations. evidence for and against each of these theories is discussed. local concentration of a variety of hormones, including human chorionic gonadotropin (hcg), sex steroids, alpha-feto ...197992318
intracellular distribution of dopa and 5-s-cysteinyldopa in pigment cells with minimal pigment formation.the hypothesis that only melanosomal catecholic amino acids contribute to melanin formation was tested by studying adult bovine eyes in which pigment synthesis is considered to be low or absent. dopa and 5-s-cysteinyldopa were investigated in different cell fractions of the choroid and retinal pigment epithelium of cattle. most of the dopa and 5-s-cysteinyldopa was found in the cytoplasm and very little in the large granule fraction. the presence of cysteinyldopa in the adult eye is evidence of ...197992151
effect of 6-hydroxydopamine on baroreceptor-heart rate and nasopharyngeal reflexes of the rabbit. 197994399
[study on the pathogenecity of chrysosporium species (author's transl)]. 197994426
study of calcium dipicolinate release during bacterial spore germination by using a new, sensitive assay for dipicolinate.the release of calcium and dipicolinic acid from spores of bacillus megaterium km during l-alanine-induced triggering of germination has been studied using a new, simple, and rapid assay for dipicolinic acid capable of detecting a concentration of 0.5 micron. the release of both calcium and dipicolinate started within seconds of exposure of the spores to l-alanine, thus preceding other measurable changes associated with germination. from the earliest times, the two substances were released in eq ...197897264
neutral protease activity in lymphocytes of lewis rats with acute experimental allergic encephalomyelitis.lymphocytes from popliteal and inguinal lymph nodes of lewis rats with acute eae as a result of injection of lyophilized guinea pig myelin in freund's complete adjuvant exerted strong proteolytic activity at neutral ph toward myelin basic protein. after injection of myelin the level of proteolytic activity remained about the same as that in lymphocytes from freund's adjuvant-injected controls until about day 10 after injection, just before the onset of paralytic symptoms; then the proteolytic ac ...197994652
hla types in corneal diseases.evaluation of the results of hla typing of 187 patients grouped into herpetic keratitis (37), non-herpetic keratitis (43), keratoconus (42), endothelial dystrophy (23), stromal dystrophy (13), lues (5), and injuries (24), failed to show convincing deviations in any of the groups from a normal control series (2900 persons). yet, as for the herpes group, a rise in b5 must strongly be suspected. the data are presented and possible implications are discussed.197994740
demonstration of specific antigenecity of various fractions of human liver homogenate. 197994039
characterization of the effects of mg2+ on ca2+- and sr2+-activated tension generation of skinned rat cardiac fibers.submaximum and maximum forces of the cardiac muscle contractile apparatus, activated by ca2+ or sr2+, were determined as a function of mg2+ concentration. apical left ventricular tissue from sprague-dawley rats was broken by homogenization into small bundles of fibers with disrupted sarcolemmas (skinned). tension generation was activated by and graded according to the concentration of ca2+ or sr2+ in solutions bathing the skinned fibers and measured with a photodiode force transducer. steady-sta ...197897362
animal oral pigmentations. 197897376
mechanism of binding of mouse interferon to controlled pore glass.many proteins bind to controlled pore glass; they are either acid elutable or alkali elutable. mouse interferon is an acid-elutable protein. since poly(l-lysine) and, to some extent, poly(l-arginine) are also eluted from controlled pore glass under acidic conditions, one may postulate that mouse interferon binds to controlled pore glass via some of the protein's epsilon-amino groups (of lysine) and/or guanidinium groups (of arginine) and the beads' silanol (hydroxyl groups). the necessity of lys ...197994835
flow cytometry: general principles and applications to selected studies in tumor biology.the cell populations derived from normal tissues and solid tumors comprised many different cell types. within each cell type there is a distribution of cells in different phases of the cell cycle and/or metabolic states (ie, differing rates of protein, rna, and other macromolecular syntheses). flow cytometry and companion instrumentation now promise to aid in rapid quantitative analyses of heterogeneous cell populations, thus finding broad applicability in many areas of cancer research and treat ...197995452
mr. pechsniff's horse? (psychodynamics in compulsive drug use). 197797538
[the appearance of penicillin resistant gonococci. clinical, genetic and epidemiologic problems]. 197896408
isolation care of infection-prone burn experimental and clinical study of spread of colonisation between burn patients, and their susceptibility to infection, was performed. burn patients' polymorphonuclear neutrophil granulocytes (pmn) functioned poorly, particularly during the second week after injury which coincided with maximum growth of bacteria in the burn wound. patients with large burns often dispersed s. aureus and ps. aeruginosa but also beta-hemolytic streptococcus to the air of their rooms. airborne transfer of these b ...197897776
localization of primate calls by old world monkeys.monkeys (macaca) were trained by operant conditioning techniques to report the minimum detectable change in location of a sound in space, and were tested with a series of recorded coo or clear call vocalizations. acuity of localization varied from approximately 4 degrees to 15 degrees and was a function of the magnitude of the change in pitch (frequency modulation) of the different clear calls.197897785
alterations of intercellular junctions in acinic cell carcinoma of the canine pancreas.intercellular junctions in spontaneous canine pancreatic acinic cell adenocarcinomas were compared to those in control canine pancreas. the neoplastic cells displayed proliferation and fragmentation of tight junctions and reduction in size and number of gap junctions. marked decrease in desmosomal density was observed only in the poorly differentiated carcinoma. in the well differentiated carcinomas a few of the desmosomes were characteristic of those found in squamous cells. no quantatitive or ...197897855
electron microscopic morphometric analysis of human t and b peripheral blood lymphocytes.using the system of morphometric analysis described in this paper, human peripheral blood t and b lymphocytes, labeled with specific surface markers, can be compared on different analytical levels. they show differences in their surface and the eccentricity of cells, in the relative surfaces occupied by peripheral and central condensed chromatin, in the average surface of the central chromatin clumps and in the number of perichromatin granules per nuclear surface. the morphometric analysis revea ...197897857
chromosomal locations of mouse immunoglobulin genes.the chromosomal locations of the structural genes coding for the constant portions of mouse heavy (h) and light chain immunoglobulins were studied by molecular hybridization techniques. complementary dna probes containing the constant-region sequences of kappa and lambdai light chain and alpha, gamma2b, and mu heavy chain mrnas were annealed to a large excess of dna from a series of eight mouse-human hybrid cell lines that are deficient for various mouse chromosomes. the lines were scored as pos ...197896442
menstruation in intraocular endometrial transplants in the rhesus monkey. 197898044
antifetal and tumor-specific reactivity in human osteosarcoma. 197994187
changes in ovarian activity during hepatocarcinogenesis by n-2-fluorenylacetamide: role of alpha-fetoprotein.during chemical hepatocarcinogenesis by n-2-fluorenylacetamide, the hormonal status of female sprague dawley rats is largely modified. ovaries present a reduced activity which is evidenced by a decreased number of follicles and corpora lutea. the level of progesterone in n-2-fluorenylacetamide treated rats is low and agrees with the lack of corpora lutea. conversely, estradiol level remains normal. this fact may be explained by the presence of alpha-fetoprotein, a carcino-embryonic protein which ...197994191
analysis of anti-sperm sera for t/t locus-specific objective antiglobulin radioimmunoassay for the analysis of anti-spermatozoa antisera has been used to characterize more than 30 alloantisera. each of these antisera was raised against spermatozoa from mice carrying t/t regin mutations. whilst all of the antisera had considerable activity for mouse spermatozoa, none of them was found to contain antibodies specific for t/t region gene products. a detailed analysis of a single serum revealed that its activity is for antigens which are non-polym ...197995255
bacillus subtilis 168 genetic transformation mediated by outgrowing spores: necessity for cell contact.transforming activity released in sequential genetic order during the first synchronous cycle of dna replication during outgrowth of spores of bacillus subtilis 168 was investigated. a transformation assay was used consisting of outgrowing spores as dna donors and multiply marked competent cells as recipients. dna synthesis inhibitors known to stop dna release were used during and subsequent to dna transfer to recipient cells. the released dna sedimented with the outgrowing cells after low-speed ...197896105
surface immunoglobulin of human lymphocytes.using ferritin as surface marker, the localization of the surface immunoglobulin (ig) was studied on peripheral lymphocytes from normal human individuals and patients with macroglobulinaemia waldenström by scanning immunoelectron microscopy. normal igg-, igm-lymphocytes and pathological igm-lymphocytes were then compared with regard to their topographical differences. in all cells examined, igg- and igm-conjugated ferritin particles were detected all over the cell surface, but the distribution o ...197896522
[effectiveness of antigens from various developmental stages of ascaris suum in the deomonstration of the migratory phase of experimental ascariasis in pigs].tests of the efficiency of antigens prepared from different developmental stages of ascaris suum in indirect haemagglutination test in the course of proving the migration phase of experimental ascariasis in pigs show that the antigens prepared by ultrasound from the invasive larval stage of a. suum in comparison with antigens of sexually mature stages have higher serological activity. by using this antigen it is possible to prove specific antibodies in experimentally invaded pigs from 6 to 120 d ...197896575
plasmid-determined resistance to hexachlorophene in pseudomonas aeruginosa.plasmid-containing strains of pseudomonas aeruginosa were screened for resistance to disinfectants. two plasmids, pmg1 and pmg2, were found to determine hexachlorophene resistance.197896729
lactase activity of microorganisms.sixty-two strains of yeasts, molds and bacteria were screened for lactase (beta-d-galactosidase) activity. strains exhibiting the enzyme activity were evaluated for cell yield as well as enzyme units available per litre of the medium, per g cell dry weight and per mg protein of their cell-free extracts. the molds exhibited lowest enzyme activity but highest cell yields, bacteria produced lowest cell yield and maximum enzyme activity. cultures exhibiting very high activity among yeasts were sacch ...197897187
[vitamin b 6 biosynthesis in bacillus subtilis].1) a vitamin-b6-producing mutant, ba 1, was selected by treatment of bacillus subtilis with n-methyl-n'-nitro-n-nitrosoguanidine. using gradient plates supplemented with the vitamin b6 antagonist isonicotinohydrazide, three mutants of ba 1 were isolated, which excrete 2-5 mg of vitamin b6/l of growth medium. 2) mutation of the three vitamin-b6-producing strains ba 1, ba 11 and l 71 led to the isolation of 49 vitamin-b6 deficient mutants. all mutants are able to grow with pyridoxine, pyridoxal, p ...197897199
influence of type of antibiotic and length of antibiotic feeding period on performance and persistence of antibiotic resistant enteric bacteria in growing-finishing swine. 197897254
antigenic variation and increase in pathogenicity in pseudomonas aeruginosa as a result of growth on glucose or n-hexadecane as sole carbon source.when pseudomonas aeruginosa is grown on glucose as opposed to n-hexadecane as the sole carbon source, the antigenicity, virulence, and protein composition of the outer membrane are altered. the hydrocarbon-grown cells demonstrate a 3-log increase in virulence over the glucose-grown cells (in mice). there also appears to be an additional protein present in the outer membrane of the n-hexadecane-grown cells. this protein may contribute to the observed antigenic differences between the two cell typ ...197896928
test for differentiation of m. tuberculosis and m. bovis from other mycobacteria.a test is described for selective inhibition of mycobacterium tuberculosis and m. bovis isolates in fluid medium. the method employs rho-nitro-alpha-acetyl-amino-beta-hydroxy-propiophenone (nap) as an inhibitory agent for differentiation of mammalian tuberculosis strains from other mycobacteria.197896930
[effect of valinomycin on mitotic activity and ciliary movement during embryonic development].the k+ ionophore valinomycin very quickly arrests cleavage in sea urchin and mouse eggs at concentrations ranging between 10 and 3 micron. development of axolotl and xenopus eggs is not arrested before the blastula or gastrula stage. the motility of sea urchin sperm, blastulae and gastrulae is suppressed, within a few minutes, by 1-9 micron valinomycin.197896956
[stimulation of pituitary lh and fsh secretion after immunoneutralization of endogenous substance p in the cycling rat].in the cycling female rat, administration of substance p antisera performed at noon on the proestrous day provokes a significant increase in lh and fsh serum level, right before or after the preovulatory surges, but had no effect on its occurrence or magnitude. these results suggest that substance p has a normal physiological inhibitory role in gonadotropin regulation.197896967
characterization and genetic control of the immune response to synthetic polypeptide antigens of defined geometry.both humoral and cell-mediated immune responses to the synthetic helical hapten-carrier conjugate poly-glu-tyr-lys(tnp)-(glu-tyr-ala)5 were found to be linked to the major histocompatibility locus in mice and guinea pigs. the responder mouse strains (h-2d haplotype) showed a primary igm response with an igg component appearing after the secondary immunization. the antibody response was accompanied by a positive dth reaction in responder strains. nonresponder mice (h-2b or h-2k haplotypes) showed ...197898587
gonorrhea detection in posthysterectomy patients.a retrospective study of women examined at the venereal disease clinic after hysterectomy was made to determine which culture site was most likely to show anogenital neisseria gonorrhoeae infection when the cervix was absent. seventeen of 104 such patients were culture positive for n gonorrhoeae. rates of positivity for our culture sites were as follows: urethral, 17/17 (100%); vaginal, 7/17 (41%); and rectal, 2/17 (12%).197898647
[protective action on mice of acellular extracts from salmonella typhimurium].acellular fractions obtained by saline extraction at 60 or at 100 degree c of salmonella typhimurium protect mice against an experimental infection with the homologous strain. after purification, these fractions which are complex, might be used for the development of a vaccine.197897021
freedom, the will-o'-the-wisp. 197898690
local cerebral energy metabolism: its relationships to local functional activity and blood flow.the results of studies with the [14c] deoxyglucose technique unequivocally establish that local functional activity in cerebral tissues is, as in other tissues, closely coupled to the local rate of energy metabolism. stimulation of local functional activity increases the local rate of glucose utilization; reduced functional activity depresses it. local cerebral blood flow is normally distributed among the cerebral structures in almost exact proportion to their rates of glucose utilization and it ...197897062
[suprachiasmatic nucleus, an hypothalamic regulation center of vital rhythmic functions]. 197897067
air caloric stimulation with tympanic membrane perforation.warm air caloric stimulation in an ear with tympanic membrane perforation or mastoidectomy cavity often causes contralateral nystagmus. secondary nystagmus is common. our evidence with squirrel monkeys and patients indicates that the primary "inversion" results from endolymph cooling due to evaporative cooling due to evaporative cooling of the mucus lining the middle ear cavity, by the dry air stimulus. disconjugate horizontal nystagmus was found in a patient with large eardrum perforation, afte ...197897478
regulation of the rate of beta-galactosidase synthese by the bgs and bgt loci in the mouse.we have developed an assay for the in vivo rate of beta-galactosidase synthesis in mouse tissues to assess the mechanism by which the bgs and bgt loci regulate activity levels of this enzyme. genetically determined differences in liver and kidney beta-galactosidase content reflect equivalent differences in specific rates of enzyme synthesis. we conclude that bgs and bgt regulate beta-galactosidase activity by controlling the rate of synthesis of the beta-galactosidase molecule.197897289
[experiments to reproduce paratyphoid in pigs of different ages]. 197898900
quantitation of particles in the freeze-fractured nuclear membrane after renal ischemia.changes in the number and sizes of membrane-associated particles have been quantitated in the protoplasmic (p) and exoplasmic (e) fracture faces of the outer membrane of nuclei isolated from the inner cortex following renal ischemia and reflow in the rat. no changes were observed in the inner nuclear membrane. after 20-min ischemia, the number of particles in both fracture faces decreased. with reflow, the total number of particles decreased after both 20- and 60-min ischemia. the partition coef ...197898907
[some aspects relating to the aflatoxin generation during the self-heating of cereals].in a stored batch of grain which was already affected by mould-formation tests were carried out with the known aflatoxin producer aspergillus flavus. a significantly lower aflatoxin production ensued if the mould growth was not connected with self-heating of the stored product. however, in conformity with increasing mould formation the germinating power was adversely affected and the significant signs (fatty acid number, reductive and none-reductive sugars) were influenced in the grain, irreleve ...197898933
immunochemical studies of the inactivation of aspartate transcarbamylase by stationary phase bacillus subtilis cells. evidence for selective, energy-dependent degradation.the aspartate transcarbamylase of bacillus subtilis is stable in exponentially growing cells, but undergoes rapid, energy-dependent inactivation when growth is inhibited by nutrient depletion or addition of antibiotics or other inhibitors of metabolism. this inactivation has been analyzed by a variety of immunochemical techniques, including direct and indirect immunoprecipitation of extracts of cells labeled with 3h-amino-acids, microcomplement fixation, and neutralization of enzymatic activity. ...197897299
jaundice and breast-feeding among alaskan eskimo newborns.the course, incidence, and severity of neonatal jaundice was studied in 95 alaskan eskimo infants. breast-fed infants had higher bilirubin concentrations than bottle-fed babies. both groups experienced high bilirubin levels, similar to those previously reported in navajo and oriental infants but greater than those observed in whites and blacks. a marked capacity to inhibit hepatic glucuronyl transferase was observed in breast-milk specimens but only partly accounted for the bilirubin differences ...197899026
separation and characterization of anti-benzylpenicilloyl (bpo) antibodies. ii. immunological properties of different igg fractions.affinity chromatography and subsequent ion-exchange chromatography of pooled anti-benzylpenicilloyl (anti-bpo) hyperimmune sera separated 5 different anti-bpo igg fractions as described in the preceding paper. these fractions were tested for activities in passive hemagglutination (pha), passive immune hemolysis (pih), antibody-dependent cellular cytotoxicity (adcc) and influence on igm-induced hemolysis. it was found that anti-bpo igg fractions with low avidity (dissociation constant k = 4.4--6. ...197897350
[intracellular oxygen and the radiosensitization of cells of the blue-green alaga, anacystis nidulans].the photosynthetic system of the blue-green alga anacystis nidulans was inactivated by 30% at a dose of 100 krad of gamma irradiation in n2. if the cells were irradiated in n2+2% co2 in the light, their radiation sensitivity was increased with an oxygen enhancement ratio of 2.0. neither light nor co2 taken separately affected radiosensitization of the cells which increased almost to the same extent as under aerobic conditions in the presence of both light and co2. the intracellular oxygen of the ...197897503
development of leucocytozoon caulleryi in chick embryos infected by biting of culicoides arakawae through shell membrane.chick embryos were infected with leucocytozoon caulleryi by biting of the midge, culicoides arakawae, through the shell membrane. schizonts of l. caulleryi were detected in the chorioallantoic membrane and most of the internal organs of embryos and of chicks after hatching. the development of schizonts was slower in embryos than in chickens. soluble antigens of l. caulleryi were demonstrated by the precipitation test in the allantoic fluid and blood from the embryos and chicks. no erythrocytic s ...197899671
[quantitative method of assessing the virulence of mycobacterium tuberculosis in an experiment on guinea pigs]. 197897664
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