| development and characterization of porphyromonas gingivalis-specific rat t-cell clones. | porphyromonas gingivalis has been implicated as a major pathogen in periodontitis. to determine the role of t cells in the regulation of this disease, a method was developed for the generation and characterization of rat t-cell clones with antigen specificity to p. gingivalis whole cells. the clones studied so far demonstrated a t-helper (th) phenotype w3/13+, w3/25+, ox8- and ox22-. these t-cell clones proliferated in vitro in response to p. gingivalis, but not to other bacteria (prevotella int ... | 1992 | 1281976 |
| antibacterial activity of dental implant metals. | this study was conducted to investigate the specific in vitro antibacterial effect of seven dental implant metals on oral bacteria which have often been identified in failing implants. the metals chosen for evaluation were titanium, chromium, cobalt, aluminum, iron, gold, and vanadium. these metals were selected because they are contained in many commonly used dental implants. the bacteria selected for this study included isolates of porphyromonas endodontalis, porphyromonas gingivalis, prevotel ... | 1992 | 1288798 |
| putative periodontal pathogens on titanium implants and teeth in experimental gingivitis and periodontitis in beagle dogs. | the microflora around titanium implants and teeth in 4 beagle dogs was analyzed in order to follow the longitudinal development from healthy conditions to experimental gingivitis and periodontitis. a 2-month plaque control program was performed to establish healthy conditions on titanium implants and control teeth (baseline, day 0). subgingival bacterial samples and radiographs were obtained. the plaque control was ceased and all measurements were repeated on day 21 (gingivitis). a new period of ... | 1992 | 1290791 |
| the relationship between clinical symptoms and anaerobic bacteria from infected root canals. | the purpose of this study was to investigate the correlation between the composition of bacterial flora from infected root canals and clinical symptoms. the materials evaluated consisted of 28 teeth from 25 patients with apical periodontitis. eubacterium were found to be significantly related to acute or chronic clinical symptoms and peptococcus, peptostreptococcus, and porphyromonas gingivalis to subacute clinical symptoms. we suggested that peptococcus, peptostreptococcus, eubacterium, porphyr ... | 1992 | 1298793 |
| characterization of total membrane protein of porphyromonas endodontalis. | porphyromonas endodontalis strains atcc 35406, hg 181, and hg 413 were cultured in an enriched broth medium, and growth curves were determined. total membrane protein profiles of cells, harvested either from exponential phase or from stationary phase of growth, were studied by sodium dodecyl sulfate polyacrylamide-gel electrophoresis. the protein profiles indicated that the three strains shared three major proteins, designated a, b, and c, with molecular masses of 59, 43, and 41 kda, respectivel ... | 1992 | 1298803 |
| role of superoxide dismutase in resistance of porphyromonas gingivalis to killing by polymorphonuclear leukocytes. | porphyromonas gingivalis in which the synthesis of superoxide dismutase (sod) was induced by nitrate or by aeration was rendered resistant to killing by polymorphonuclear leukocytes. sod purified from either anaerobically maintained or aerated cells also inhibited bacterial killing when added exogenously, and no difference between the effects of the two sods was observed. these results suggest that sod may form part of a defense mechanism that helps protect p. gingivalis against killing by polym ... | 1992 | 1309723 |
| the effect of 3 mouthrinses on plaque and gingivitis development. | the aim of this study was to evaluate the effect of 3 mouthrinses, listerine antiseptic (thymol), peridex (chlorhexidine), perimed (povidone iodine and hydrogen peroxide), and a placebo (water) on the development of dental plaque and gingivitis, when used as the only oral hygiene procedure for 14 days. 71 subjects were entered into a randomized, double-blind study. at the baseline examination, papillary bleeding score (pbs), and plaque index (pi) were registered, after which subjects received su ... | 1992 | 1310096 |
| comparison of the benzoyl-dl-arginine-naphthylamide (bana) test, dna probes, and immunological reagents for ability to detect anaerobic periodontal infections due to porphyromonas gingivalis, treponema denticola, and bacteroides forsythus. | most forms of periodontal disease are associated with the presence or overgrowth of anaerobic species that could include treponema denticola, porphyromonas gingivalis, and bacteroides forsythus among others. these three organisms are among the few cultivable plaque species that can hydrolyze the synthetic trypsin substrate benzoyl-dl-arginine-naphthylamide (bana). in turn, bana hydrolysis by the plaque can be associated with periodontal morbidity and with the presence of these three bana-positiv ... | 1992 | 1311335 |
| influence of immunization on porphyromonas gingivalis colonization and invasion in the mouse chamber model. | the effects of immunization with invasive or noninvasive porphyromonas (bacteroides) gingivalis strains on the pathogenesis of infection in a mouse chamber model were examined. balb/c mice were immunized by a single injection of heat-killed p. gingivalis invasive strain a7436 or w83 or noninvasive strain 33277, hg405, or 381 directly into subcutaneous chambers. p. gingivalis-specific antibody was detected in chamber fluid 21 days postimmunization, and mice were subsequently challenged by injecti ... | 1992 | 1312515 |
| porphyromonas gingivalis virulence in mice: induction of immunity to bacterial components. | selected cell envelope components of porphyromonas gingivalis were tested in a balb/c mouse model in an attempt to elucidate further the outer membrane components of this putative oral pathogen that might be considered as virulence factors in host tissue destruction. lipopolysaccharide (lps), outer membrane, and outer membrane vesicles of p. gingivalis w50, atcc 53977, and atcc 33277 were selected to examine an immunological approach for interference with progressing tissue destruction. mice wer ... | 1992 | 1312516 |
| biochemical characterization of porphyromonas (bacteroides) gingivalis collagenase. | a protease was purified from porphyromonas gingivalis 1101, a clinical isolate, by sequential sodium dodecyl sulfate-polyacrylamide gel electrophoresis, substrate diffusion gel electrophoresis, and electroelution. the enzyme cleaved radiolabeled human basement membrane type iv collagen and the synthetic collagen peptide substrate for eukaryotic collagenases. it was inactivated by the thiol protease inhibitor n-ethylmaleimide but not by edta or egta [ethylene glycol-bis(beta-aminoethyl ether)-n,n ... | 1992 | 1312517 |
| phagocytosis of porphyromonas gingivalis and prevotella intermedia by human polymorphonuclear leukocytes in clots formed from human plasma or purified fibrinogen. | we have studied the polymorphonuclear leukocyte (pmn) phagocytosis of porphyromonas gingivalis (bacteroides gingivalis) and prevotella intermedia (bacteroides intermedius) in three-dimensional fibrin meshworks formed by either clotting human plasma or by clotting purified fibrinogen with human serum incorporated. pmn phagocytosis of rhodamine isothiocyanate-labelled p. gingivalis in the fibrinogen clot was 43 +/- 6% and in the plasma clot was 62 +/- 10%; for p. intermedia the values were 15 +/- ... | 1992 | 1312590 |
| a sensitive enzymatic method (sk-013) for detection and quantification of specific periodontopathogens. | porphyromonas gingivalis, bacteroides forsythus, and treponema denticola have been found to predominate in periodontal pockets of patients with adult periodontitis. these microorganisms hydrolyze the synthetic peptide n-benzoyl-dl-arginine-2-naphthylamide (bana). in this study, we developed an enzymatic method, designated sk-013, to detect the existence of these microorganisms in subgingival plaque bacteria. this enzymatic method was based on the observation of the hydrolysis of n-carbobenzoxy-g ... | 1992 | 1312591 |
| a sensitive enzymatic method (sk-013) for detection of treponema denticola, porphyromonas gingivals and bacteroides forsythus in subgingival plaque samples. | an enzymatic method, sk-013, was developed for rapid detection of the peptidase activity in subgingival plaque samples. this method was found to have specificity for porphyromonas gingivalis, treponema denticola, bacteroides forsythus, and some capnocytophaga strains. the purpose of this study was to determine whether sk-013 could indicate the presence of periodontopathic bacteria, including t. denticola, p. gingivalis and b. forsythus, which produce trypsin-like enzymes. subgingival plaque samp ... | 1992 | 1312592 |
| gingival crevicular fluid concentration and side effects of minocycline: a comparison of two dose regimens. | the purpose of this study was to conduct a direct comparison of two dose regimens of minocycline to determine 1) whether they achieved crevicular fluid concentrations in a therapeutic range; and 2) the frequency of side effects. in a double-blind design, 30 patients divided into 2 groups were given either 100 mg minocycline or 200 mg minocycline per day for an 8-day period. the concentration of minocycline in the gingival clevicular fluid (gcf) at 8 days was 4.77 micrograms/ml for the 100 mg a d ... | 1992 | 1313100 |
| assessment of the relative cytotoxicity of porphyromonas gingivalis cells, products, and components on human epithelial cell lines. | established human cell lines derived from a transitional cell carcinoma (j82), a squamous carcinoma (scaber), and a normal urothelium (hcv-29) were used to assess the relative cytotoxicity and tissue specificity of putative virulence determinants of p. gingivalis w83. intact cells of w83 had no effect on any of the cell lines, whereas disrupted cells caused extensive cytotoxicity particularly to monolayers of hcv-29 and j82. the purified cysteine proteinase, gingivain, caused marked disruption o ... | 1992 | 1313102 |
| microbiological and clinical results of metronidazole plus amoxicillin therapy in actinobacillus actinomycetemcomitans-associated periodontitis. | we report on the microbiological and clinical effects of mechanical debridement in combination with metronidazole plus amoxicillin therapy in 118 patients with actinobacillus actinomycetemcomitans-associated periodontitis. patients were categorized into 3 groups: 28 had localized periodontitis; 50 had generalized periodontitis, and 40 had refractory periodontitis. after initial treatment and metronidazole plus amoxicillin therapy 114 of 118 (96.6%) patients had no detectable a. actinomycetemcomi ... | 1992 | 1313103 |
| humoral antibody responses in periodontal disease. | several forms of periodontal disease are considered to be infectious diseases with associated specific bacteria. this study examined the humoral antibody levels as assayed by elisa to actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and prevotella intermedia in adult periodontitis (ap), localized juvenile periodontitis (ljp), rapidly progressive periodontitis (rpp), and in periodontally healthy subjects (hs). sixty-two of the 64 (96.9%) patients had significantly elevated antibody ... | 1992 | 1313106 |
| evaluation of oral bacteria as risk indicators for periodontitis in older adults. | the prevalence of people and sites with attachment loss, pocket depth, actinobacillus actinomycetemcomitans, prevotella intermedia, and porphyromonas gingivalis are described for a random sample of 366 black and 297 white community-dwelling adults, aged 65 or over, residing in five counties in north carolina. in addition, relationships between sites harboring these microorganisms and loss of attachment (la) and pocket depth (pd) are presented in a manner that considers the lack of independence o ... | 1992 | 1313107 |
| a statistical approach to the ecology of porphyromonas gingivalis. | previous studies have suggested that infections with porphyromonas gingivalis, associated with periodontal disease, may consist of one clonal type. it has also been shown that each individual patient carries a unique clonal type of p. gingivalis, as assessed by dna fingerprinting. this issue was further examined by random collection of multiple isolates of p. gingivalis from multiple sites in several patients, and characterization of these isolates by dna fingerprinting. although most patients a ... | 1992 | 1313462 |
| evidence for independent molecular identity and functional interaction of the haemagglutinin and cysteine proteinase (gingivain) of porphyromonas gingivalis. | the sequence of events involved in haemagglutination and lysis of erythrocytes by washed cells, vesicles and the culture supernate of porphyromonas gingivalis strain w83 was monitored by 51cr release and transmission electronmicroscopy. all preparations, except capsular material and lipopolysaccharide, caused haemagglutination and, by a slow process of attachment and specific attack on the surface structures of the red blood cells, produced minute pores and eventual leakage of cellular contents. ... | 1992 | 1313881 |
| inactivation of human serum bactericidal activity by a trypsinlike protease isolated from porphyromonas gingivalis. | a protease was isolated from an outer membrane vesicle preparation of porphyromonas gingivalis atcc 33277 and assessed for its ability to inactivate the bactericidal activity of normal human serum. the enzyme, which was activated by reducing agents, was found to be a trypsinlike protease with a molecular mass of approximately 80 kda. prior to being tested in the bactericidal assay, pooled human serum was preincubated with the partially purified enzyme. under conditions in which the trypsinlike p ... | 1992 | 1314221 |
| treponema denticola and porphyromonas gingivalis as prognostic markers following periodontal treatment. | subgingival plaque samples were collected from individuals with advanced periodontitis before and 3 to 11 weeks after scaling and root planing periodontal treatment. the plaque levels of treponema denticola and porphyromonas gingivalis antigens were measured before and after treatment by a quantitative immunoassay procedure using monoclonal antibodies specific for these oral bacteria. a decrease in mean levels of t. denticola (p less than .05) and p. gingivalis antigens (p less than .09) were ob ... | 1992 | 1315388 |
| microbiology of healthy and diseased periodontal sites in poorly controlled insulin dependent diabetics. | a group of poorly-controlled insulin dependent diabetes mellitus (iddm) patients were examined in a cross-sectional design for total microbial levels, microbial incidence, and the percent levels of selected periodontal microorganisms. these organisms were selected on the basis of prior reports that associated them with either periodontal disease or health. one periodontally-healthy and one periodontally-diseased site were examined in each iddm patient. increased levels of the periodontal pathoge ... | 1992 | 1315389 |
| serum immunoglobulin g antibody to porphyromonas gingivalis in rapidly progressive periodontitis: titer, avidity, and subclass distribution. | porphyromonas gingivalis is a suspected pathogen in rapidly progressive periodontitis (rpp). we have determined the anti-p. gingivalis serum immunoglobulin g (igg) isotype response and avidity and the subclass titer distributions for 30 rpp patients and 30 age-, sex-, and race-matched healthy subjects by using enzyme-linked immunosorbent assay technology. patients and control subjects were classified as seropositive if their total igg response to p. gingivalis was twofold or more than the median ... | 1992 | 1316874 |
| the occurrence of periodontitis-related microorganisms in relation to titanium implants. | the peri-implant space was examined for the presence of periodontitis-related microorganisms in 19 patients. all patients had been fitted with osseointegrated titanium implants which were clinically sound at the time of examination and all had been prescribed daily 0.2% chlorhexidine mouthrinse. in 17 patients, two contralateral fixtures in patients were selected, the remaining 2 patients had only one fixture, and associated mucosal clinical variables assessed for plaque accumulation, gingival i ... | 1992 | 1317427 |
| sequence analysis and characterization of the porphyromonas gingivalis prtc gene, which expresses a novel collagenase activity. | in order to examine the potential role of bacterial collagenases in periodontal tissue destruction, we recently isolated a gene, prtc, from porphyromonas gingivalis atcc 53977, which expressed collagenase activity (n. takahashi, t. kato, and h. k. kuramitsu, fems microbiol. lett. 84:135-138, 1991). the nucleotide sequence of the gene has been determined, and the deduced amino acid sequence corresponds to a basic protein of 37.8 kda. in addition, southern blot analysis indicated that the prtc gen ... | 1992 | 1317840 |
| enhanced gingivitis in the deciduous and permanent dentition. an experimental study in the dog. | the aim of the experiment was to analyze the reaction of the marginal gingival tissues to 21 days of plaque formation on buccal tooth surfaces in the deciduous and permanent dentition of beagle dogs. in order to enhance the formation of plaque, the buccal surfaces of the experimental teeth were coated with a composite filling material. 5 beagle dogs were used. the animals were monitored during 2 periods, called period a (42 days during the deciduous dentition) and period b (42 days during the pe ... | 1992 | 1318329 |
| the use of dna probes to examine the distribution of subgingival species in subjects with different levels of periodontal destruction. | the present investigation examined the distribution of 14 subgingival species at a total of 2299 sites in 90 subjects with different levels of periodontal destruction. subgingival plaque samples taken from the mesial aspect of each tooth were anaerobically dispersed, diluted and plated on non selective media. after anaerobic incubation, colonies were lifted to nylon filters and specific species detected using digoxygenin-labeled whole chromosomal dna probes. the mean total viable count for all s ... | 1992 | 1318331 |
| immunoglobulin-degrading enzymes in localized juvenile periodontitis. | previous reports have indicated the association of periodontal diseases with elevated levels of serum immunoglobulin g (igg) antibodies to periodontally relevant bacteria. recent results from this laboratory suggest that enzymes proteolytic for immunoglobulins are important virulence factors of several periodontal bacteria. specifically, enzymes from porphyromonas (bacteroides) gingivalis culture supernatant fluid (sf) cleaved human igg (4 subclasses), iga1 and iga2, igm, igd and ige. proteolyti ... | 1992 | 1318974 |
| gene cloning of porphyromonas gingivalis specific antigens recognized by serum of adult periodontitis patient. | 1. porphyromonas gingivalis is believed an important pathogen of adult periodontitis. a gene library of p. gingivalis 381 was constructed in lambda phage vector l47.1. the library was probed with serum obtained from patients of severe adult periodontitis. two clones, lambda mdbg101 and lambda mdbg103 which were expressed, 200 and 160 kda respectively, were selected and further studied. 2. the expressed antigens in these two clones were also reacted with rabbit antiserum against whole cells, caps ... | 1992 | 1319357 |
| the introduction of colonic-bacteroides shuttle plasmids into porphyromonas gingivalis: identification of a putative p. gingivalis insertion-sequence element. | two escherichia coli-bacteroides plasmid-shuttle vectors pnjr5 and pnjr12 were introduced for the first time into porphyromonas gingivalis w83 by conjugal transfer from e. coli. the transfer frequencies were comparable to those obtained when using colonic bacteroides as recipients. both plasmids were maintained in p. gingivalis w83 and could be isolated and introduced back into e. coli. plasmid dna extracted from one p. gingivalis w83 pnjr12 transconjugant had an additional 1.5 kb of inserted dn ... | 1992 | 1319376 |
| inhibition of porphyromonas gingivalis adhesion to streptococcus gordonii by human submandibular-sublingual saliva. | porphyromonas gingivalis w50 adheres in vitro to biofilms of streptococcus gordonii g9b. this phenomenon is believed to facilitate the initial colonization of the oral cavity by p. gingivalis and to contribute to the maturation of dental plaque. in this report, we describe the modulating effects of human submandibular-sublingual saliva (hsmsl) on this in vitro model of intergeneric bacterial adhesion (coaggregation). hsmsl inhibited p. gingivalis adhesion to s. gordonii by 50% at a concentration ... | 1992 | 1319402 |
| molecular cloning and expression of a major surface protein (the 75-kda protein) of porphyromonas (bacteroides) gingivalis in escherichia coli. | a major immunodominant surface protein (the 75-kda protein) of porphyromonas (bacteroides) gingivalis 381 has been purified and its amino-terminal amino acid sequence has been determined. using oligonucleotide probes corresponding to the sequence, we identified a recombinant plasmid clone carrying a single 4.2-kb bamhi fragment from puc19 libraries of p. gingivalis. the bamhi fragment transferred to the bacteriophage t7 rna polymerase/promoter expression vector system produced a slightly larger ... | 1992 | 1320586 |
| cloning of a porphyromonas (bacteroides) gingivalis protease gene and characterization of its product. | a clone expressing a porphyromonas gingivalis protease from the recombinant plasmid (pys307) has been identified in a genomic library of p. gingivalis w83. the cloned gene was localized to a 2.4-kb dna fragment between bamhi and hindiii sites. when a 3.2-kb hindiii fragment of pys307 was used as a probe in southern hybridization, hindiii-digested chromosomal dna of p. gingivalis w83, as well as those of w50 and w12, showed a single 3.2-kb hybridizing band, while that of p. gingivalis 33277 showe ... | 1992 | 1320588 |
| humoral igg antibodies to oral microbiota in a population at risk for root-surface caries. | mutans streptococci have been strongly implicated in the initiation of dental caries on coronal surfaces. their role in development of root-surface caries is less clear. the etiologic agents of both types of dental caries are likely to elicit systemic immune responses. the objective of the present study, therefore, was to study the association of clinical variables of disease with humoral igg antibodies to nine oral micro-organisms in 314 adult subjects, aged 45-65 years, who were at risk for ro ... | 1992 | 1321176 |
| migration and chemiluminescence of polymorphonuclear cells and monocytes to bacteroides sonicates. | recent investigations have demonstrated that various preparations obtained from representatives of the genus bacteroides are poorly phagocytized by polymorphonuclear cells (pmn) and macrophages. crude cell sonicates derived from bacteroides have been examined for their ability to inhibit migration of pmn and monocytes using a modified migration under agarose in vitro assay. b. gingivalis and b. intermedius were found to be inhibitors of such migration while b. asaccharolyticus did not share this ... | 1992 | 1321583 |
| subgingival temperature (iii). relation to microbial counts. | the present investigation examined the relationship of selected bacterial species and subgingival temperature. 35 subjects were measured at 6 sites per tooth for clinical parameters and subgingival temperature. measurements were repeated for 21 subjects at 2 month intervals providing a total of 66 subjects visits. at each visit, subgingival plaque samples were taken from the mesial aspect of each tooth and anaerobically dispersed, diluted and plated on non-selective media. after anaerobic incuba ... | 1992 | 1321846 |
| cloning, expression, and sequencing of a protease gene (tpr) from porphyromonas gingivalis w83 in escherichia coli. | porphyromonas gingivalis is a highly proteolytic organism which metabolizes small peptides and amino acids. indirect evidence suggests that the proteases produced by this microorganism constitute an important virulence factor. in this study, a gene bank of p. gingivalis w83 dna was constructed by cloning 0.5- to 20-kb hindiii-cut dna fragments into escherichia coli dh5 alpha by using the plasmid vector puc19. a clone expressing a protease from p. gingivalis was isolated on lb agar containing 1% ... | 1992 | 1322368 |
| conjugal transfer of plasmid and transposon dna from escherichia coli into porphyromonas gingivalis. | using the broad host-range vector r751 to provide transfer functions, plasmid pval-1 and transposon tn4351 were conjugally mobilized from escherichia coli into porphyromonas gingivalis. transfer frequencies for both elements varied between 10(-6) and 10(-11), depending upon the recipient. the behavior of pval-1 and tn4351 in p. gingivalis was essentially as described previously in bacteroides spp. these data indicate that plasmid and transposon dna can be conjugally transferred into p. gingivali ... | 1992 | 1323280 |
| humoral response to porphyromonas (bacteroides) gingivalis in rats: time course and t-cell dependence. | in this study, we describe the time course and t-cell dependence of the serum antibody response to the periodontopathogen porphyromonas (bacteroides) gingivalis in an experimental rat model. normal fischer rats were challenged by a local injection of p. gingivalis (2 x 10(8) bacteria) into gingival tissue or by the administration of a similar number of bacteria by the intravenous (i.v.) route on days 0, 2, and 4. serum antibody activity was detected within 1 week and peaked at 8 weeks after ging ... | 1992 | 1323534 |
| e-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms. | the purpose of the study was to validate the epsilometer test (e-test) method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard. the e-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. the device consists of a predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip. after 48 hours incub ... | 1992 | 1324301 |
| development of a rapid latex agglutination test for periodontal pathogens. | the studies reported here describe the development, characterization, and initial application of latex agglutination assays for periodontal pathogens. latex reagents were prepared by sensitization of latex microspheres with rabbit igg antibodies to either actinobacillus actinomycetemcomitans, porphyromonas gingivalis, or prevotella intermedia. the protein concentration utilized for sensitization and microsphere size were optimized. one reagent was prepared to a. actinomycetemcomitans and a secon ... | 1992 | 1324302 |
| synergistic effect of pyrophosphate and sodium dodecyl sulfate on periodontal pathogens. | our previous studies have shown that pyrophosphate (ppi), the anticalculus component of tartar-control dentifrices, inhibits the growth of organisms associated with coronal and root surface caries. the purposes of this investigation were to: 1) determine if periodontal pathogens are similarly susceptible to the growth-inhibitory properties of ppi; and 2) determine if combinations of pyrophosphate-sodium dodecyl sulfate (ppi-sds) inhibit growth synergistically. porphyromonas gingivalis, actinobac ... | 1992 | 1324304 |
| a preliminary study on the effects of the nd:yag laser on root surfaces and subgingival microflora in vivo. | the purpose of this study was to examine the effects of root preparation using the pulsed nd:yag laser, either alone or in combination with manual instrumentation. study specimens consisting of 18 teeth with associated periodontal pockets from 8 different patients were treated as follows: 5 specimens were root planed with curets followed by laser exposure for 3 minutes using energy settings of 3.0 w at 20 pps; 2 specimens were root planed and then laser treated for 3 minutes using settings of 2. ... | 1992 | 1324305 |
| transposition of tn4351 in porphyromonas gingivalis. | genetic analysis of porphyromonas gingivalis, an obligately anaerobic gram-negative bacterium, has been hindered by the apparent lack of naturally occurring bacteriophages, transposable elements, and plasmids. plasmid r751::*omega 4 has previously been used as a suicide vector to demonstrate transposition of tn4351 in b. uniformis. the erythromycin resistance gene on tn4351 functions in bacteroides and porphyromonas. erythromycin-resistant transconjugants were obtained at a mean frequency of 1.6 ... | 1992 | 1325062 |
| susceptibility of porphyromonas gingivalis and p. asaccharolytica to the non-oxidative killing mechanisms of human neutrophils. | neutrophils are essential for host defence against bacterial dental plaque and the pathogenic bacterial species within it, but in anaerobic environments such as the gingival crevice neutrophils can kill bacteria only with non-oxidative microbicidal compounds stored in their granules. porphyromonas gingivalis w83, a pathogenic plaque species, and the avirulent non-oral type-strain p. asaccharolytica were incubated anaerobically with intact neutrophils and with compounds extracted from normal huma ... | 1992 | 1325146 |
| anti-proliferative and cytotoxic activity of surface-associated material from periodontopathogenic bacteria. | the easily solubilized surface-associated material from three bacterial species associated with periodontal diseases, actinobacillus actinomycetemcomitans, porphyromonas gingivalis and eikenella corrodens, produced dose-dependent inhibition of thymidine incorporation by human fibroblasts, the human monocytic cell line u937 and guinea pig epidermal cells. in contrast, lipopolysaccharides from a. actinomycetemcomitans and p. gingivalis were either inactive or substantially less active over the dos ... | 1992 | 1325147 |
| inhibition of peptidase and glycosidase activities of porphyromonas gingivalis, bacteroides intermedius and treponema denticola by plant extracts. | aqueous extracts from 5 plants used widely in kenya as chewing sticks (mswaki) for the control of oral hygiene were tested for their ability to inhibit extracellular peptidase and glycosidase enzyme activities produced by the periodontopathic bacteria porphyromonas gingivalis (formerly bacteroides gingivalis), bacteroides intermedius and treponema denticola. the plants studied were rhus natalensis, cupressus hisitanica, sida cordifolia, olea africana and euclea divinorum. protease activities, in ... | 1992 | 1325483 |
| supragingival cleaning 3 times a week. the microbiological effects in moderately deep pockets. | it is generally believed that supragingival plaque control has little effect on the subgingival microflora of deep periodontal pockets. however, this may not be true for moderately deep pockets (4-5 mm), which may represent a pathological state between gingivitis and marginal periodontitis. in 6 patients with poor oral hygiene and severe gingival inflammation, 4 matched sites (1 in each quadrant), were chosen which demonstrated at least 20% spirochetes and 15% black-pigmented gram-negative bacil ... | 1992 | 1325484 |
| microbial etiology of periodontal diseases. where are we? where are we going? | many studies indicate a strong association of actinobacillus actinomycetemcomitans with localized juvenile periodontitis. species associated with adult periodontitis include bacteroides forsythus, porphyromonas gingivalis, prevotella intermedia, a. actinomycetemcomitans, and wolinella recta. capnocytophaga species may be important in pubertal gingivitis. an unnamed spirochete related to treponema pallidum has been identified in acute necrotizing ulcerative gingivitis lesions. species isolated fr ... | 1992 | 1325846 |
| application of polymerase chain reaction with arbitrary primer (ap-pcr) to strain identification of porphyromonas (bacteroides) gingivalis. | several molecular methods are currently available for identification and discrimination of bacterial strains within the same species, which vary in efficiency and required labour. here we applied a novel method for fingerprinting genomes, called arbitrarily primed pcr (ap-pcr), to the delineation of strains within the species porphyromonas gingivalis. using a single primer on a set of nine strains, nine simple distinct banding patterns, indicative of genetic polymorphism, were observed. common a ... | 1992 | 1326466 |
| bacterial synergy of treponema denticola and porphyromonas gingivalis in a multinational population. | treponema denticola and porphyromonas gingivalis have been associated with human adult severe periodontitis. in this study, we quantified these putative pathogens in subgingival plaque samples collected from 74 fijians, 74 colombians and 73 u.s. americans stationed at the multinational force and observers encampment in the sinai desert, egypt. a contingency table of t. denticola and p. gingivalis frequency revealed a highly significant synergistic relationship. we discovered that the occurrence ... | 1992 | 1326739 |
| immunochemical and biological characterization of outer membrane proteins of porphyromonas endodontalis. | outer membrane proteins (omp) of porphyromonas endodontalis hg 370 (atcc 35406) were prepared from the cell envelope fraction of the organisms. the cell envelope that had been obtained by sonication of the whole cells was extracted in 2% lithium dodecyl sulfate and then successively chromatographed with sephacryl s-200 hr and deae-sepharose fast flow. two omp fractions, omp-i and omp-ii, were obtained, and their immunochemical properties and induction of specific antibodies were examined. the om ... | 1992 | 1328059 |
| lipopolysaccharides of bacteroides intermedius (prevotella intermedia) and bacteroides (porphyromonas) gingivalis induce interleukin-8 gene expression in human gingival fibroblast cultures. | lipopolysaccharides (lps) prepared from bacteroides intermedius (prevotella intermedia) and bacteroides (porphyromonas) gingivalis by hot phenol-water extraction induced interleukin-8 (il-8) mrna in normal human gingival fibroblast cultures, as demonstrated by northern (rna) blot analysis. il-8 mrna levels began to increase after a 2-h exposure, reached a maximum after 12 h, and then dropped to the unstimulated level at 48 h. il-8 mrna levels were also enhanced in a dose-dependent manner. by con ... | 1992 | 1328062 |
| dentin as inhibitor of bacterial toxicity on pulpal cells in vitro. | many studies have shown that the major cause of pulpal disease is the presence of bacteria or their by-products in the dentinal tubules. the purpose of this investigation was to develop an in vitro model, simulating the pulp chamber, that would permit the study of the transport of bacterial by-products through dentin and their effect on pulpal cells. human pulpal cells were cultured in a modified sykes-moore chamber and exposed through dentin to sonicated extracts of porphyromonas gingivalis atc ... | 1992 | 1328455 |
| comparative study of subgingival microbiological sampling techniques. | the presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease. the technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported. in this study, paper point sampling and scaler sampling are compared. subgingival samples using both methods were taken from three surfaces in each of 12 patients at the following time points: at each of two appointments one week apart before treatment a ... | 1992 | 1328591 |
| longitudinal monitoring for disease progression of localized juvenile periodontitis. | the present study was performed to investigate the factors associated with disease progression in localized juvenile periodontitis (ljp) patients by longitudinal monitoring of microbiological changes. following a 9-month period, 9 ljp patients were divided into 2 groups based upon attachment loss, progressing and non-progressing. both groups received scaling, root planing, and modified widman flaps. clinical and microbiological data were obtained at baseline, following the observation period, an ... | 1992 | 1328592 |
| effects of a bisphosphonate on experimental periodontitis in monkeys. | bisphosphonates have been shown to increase bone mass in estrogen-deficient patients by inhibiting osteoclast activity. the purpose of this study was to measure clinical and radiographic effects of a bisphosphonate on periodontitis development in monkeys. twenty-seven (27) adult cynomolgus monkeys were studied. after quarantine, baseline data were obtained including plaque index, gingival index, clinical probing depth measurements, and intraoral radiographs. standardized radiographs were analyze ... | 1992 | 1328593 |
| characterization of the adherence of porphyromonas gingivalis to oral streptococci. | adherence of porphyromonas gingivalis to strains of streptococcus sanguis and streptococcus mitis deposited on nitrocellulose paper was investigated. a variety of laboratory strains and clinical isolates of p. gingivalis bound to both s. sanguis and s. mitis. binding of p. gingivalis to all but one of the streptococci was not inhibited by salivary molecules. pretreatment of p. gingivalis with periodate and pretreatment of s. sanguis and s. mitis with pronase decreased binding, suggesting that ad ... | 1992 | 1328995 |
| inhibition of actinomyces viscosus--porphyromonas gingivalis coadhesion by trypsin and other proteins. | protease activity is associated with the coadhesion of actinomyces viscosus and porphyromonas gingivalis. to try to distinguish whether the recognition/adhesion or degradative functions of proteases are more crucial for coadhesion, we determined the effect of trypsin and other purchased proteases and proteins on coadhesion when they were incorporated in the coadhesion assay buffer or when a. viscosus cells were pretreated with trypsin. coadhesion was measured by the decrease in turbidity caused ... | 1992 | 1328996 |
| survey of a receptor protein in human erythrocytes for hemagglutinin of porphyromonas gingivalis. | the purpose of this study is to survey a receptor protein in human erythrocyte membrane for the hemagglutinin (ha) of porphyromonas gingivalis. human erythrocytes were modified by either chymotrypsin or p. gingivalis ha along with the disappearance of their hemagglutinating ability and the removal of the band 3 protein. by preparative electrophoresis, this protein was isolated and purified from human erythrocytes. the purified protein showed strong inhibitory activity for hemagglutination and th ... | 1992 | 1328997 |
| comparative studies of three proteases of porphyromonas gingivalis. | three thiol-activated proteases, designated qa, ra, and sa, in the soluble fraction of the cell extract of porphyromonas gingivalis atcc 33277 were purified by combinations of gel filtration, ion exchange chromatography and electrophoresis, and characterized. the molecular weights estimated by gel filtration method were 43 kda (sa), 87 kda (ra), and 170 kda (qa). however, they were found to have the same molecular weight (43 kda), when estimated by sds-page, indicating that sa is a monomeric, ra ... | 1992 | 1328998 |
| direct and indirect effects of porphyromonas gingivalis lipopolysaccharide on interleukin-6 production by human gingival fibroblasts. | we examined the production of interleukin-6 (il-6) by human gingival fibroblasts (atcc crl 1292) stimulated with lipopolysaccharide (lps) from porphyromonas gingivalis and escherichia coli, or supernatant of human peripheral blood adherent cell culture medium incubated in the presence of il-1 and the same two lps. confluent monolayers of gingival fibroblasts were incubated with stimulants for 6 h at 37 degrees c in 5% co2 and air. after removal of stimulants, the cell cultures were incubated for ... | 1992 | 1328999 |
| role of porphyromonas gingivalis 40-kda outer membrane protein in the aggregation of p. gingivalis vesicles and actinomyces viscosus. | porphyromonas gingivalis, an important pathogen in periodontitis, produces extracellular vesicles that aggregate with actinomyces viscosus cells. a 40-kda outer membrane protein (omp)-coding gene from p. gingivalis was cloned and the protein was found to be localized in these vesicles. the recombinant 40-kda omp did not show aggregation activity. however, affinity-purified antibody against the recombinant protein significantly inhibited aggregation of p. gingivalis vesicles with a. viscosus cell ... | 1992 | 1329700 |
| igg subclass antibodies to porphyromonas gingivalis in patients with destructive periodontal disease. a case: control study. | we have estimated by elisa the levels of igg subclass antibodies against p. gingivalis strain w83 whole cells in 35 cases of adult periodontitis and 35 age, sex, ethnic origin and plaque index-matched healthy controls. the mean levels of igg1 (p < 0.0003) and igg2 (p < 0.0416) were significantly elevated in the cases. many sera had no detectable igg4 antibodies but by categorising igg4 responses into high, low and absent, this subclass was more often present (p < 0.002) in the cases. analysis of ... | 1992 | 1331202 |
| occurrence of periopathogens in smoker and non-smoker patients. | the aim of the present study was to elucidate the interrelationship between cigarette smoking and the occurrence of periopathogens, i.e., actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius. the study was based on 145 patients with clinically severe periodontitis, 52 men (age range 32-73 years) and 93 women (age range 32-74 years). 83 patients were smokers (> or = 15 cigarettes/day) and 62 non-smokers. bacterial samples were collected from one site with a pro ... | 1992 | 1331203 |
| effect of porphyrins and host iron transport proteins on outer membrane protein expression in porphyromonas (bacteroides) gingivalis: identification of a novel 26 kda hemin-repressible surface protein. | porphyromonas gingivalis is capable of in vitro growth when iron sources are either complexed to hemin or host iron transport proteins, or exist in an inorganic form. this study examined the effect of these iron sources on outer membrane protein (omp) expression in p. gingivalis w50. hemin (iron) starved p. gingivalis was transferred into growth medium containing hemin, hemoglobin, hemin-saturated human serum albumin, hemin-free human serum albumin, transferrin, lactoferrin, or inorganic iron. s ... | 1992 | 1331700 |
| cloning of the gene encoding a glycylprolyl aminopeptidase from porphyromonas gingivalis. | a genomic library of porphyromonas gingivalis 381 was constructed in the cosmid vector phc79. a clone, psn1, was identified by the expression of glycylprolyl-naphthylamide hydrolysing activity. the dna insert contained within the cosmid psn1 was subcloned into the plasmid vector pbr328 to create the recombinant plasmid psn11 containing a 2.9 kb ecorv insert. an escherichia coli transformant containing psn11 produced a protein having a molecular weight of 75 kda. southern-blot hybridization revea ... | 1992 | 1332661 |
| generation of plasma kinin by three types of protease isolated from porphyromonas gingivalis 381. | three types of protease (a, b and c) isolated from the culture supernatant of porphyromonas gingivalis 381 had peculiar activities on kinin generation from high molecular-weight kininogen in vitro. protease c released bradykinin from the kininogen in a reaction mixture containing 2 mm dithiothreitol, but a and b did not. however, the activity of degrading bradykinin was much stronger in protease a and b than in c. these findings suggest that only protease c shows plasma kallikrein activity. | 1992 | 1332662 |
| correlation of haemagglutination activity with trypsin-like protease activity of porphyromonas gingivalis. | porphyromonas gingivalis is a gram-negative anaerobic bacterium associated with various forms of periodontal disease. several characteristics of p. gingivalis are thought to contribute to its pathogenicity; these include haemagglutination and trypsin-like protease activity. previous studies suggest an association between haemagglutination and trypsin-like protease activity of p. gingivalis. to investigate this, two complementary quantitative experimental approaches were taken. five independent m ... | 1992 | 1332663 |
| comparative clinical and microbiological effects of topical subgingival application of metronidazole 25% dental gel and scaling in the treatment of adult periodontitis. | the aim of the study was to compare the clinical and microbiological effects of topical application of a metronidazole gel and a single session of subgingival scaling in the treatment of adult periodontitis. an open, randomized controlled clinical study design was employed. each of 24 subjects received the 2 treatments simultaneously each in 2 randomly selected quadrants of the dentition. the metronidazole, 25% gel was applied subgingivally on days 0 and 7. scaling was carried out in one quadran ... | 1992 | 1332988 |
| nutritional interactions between two suspected periodontopathogens, treponema denticola and porphyromonas gingivalis. | a mutual symbiotic enhancement of growth of porphyromonas gingivalis and treponema denticola is described in this report. brain heart infusion broth supplemented with vitamin k did not support the individual growth of p. gingivalis or t. denticola. however, when inoculated as a mixture, both bacterial species did grow significantly. the growth-stimulating factors produced by p. gingivalis and t. denticola were dialyzable and heat stable and were further identified as isobutyric acid and succinic ... | 1992 | 1333450 |
| refractory periodontitis associated with abnormal polymorphonuclear leukocyte phagocytosis and cigarette smoking. | to learn if refractory periodontitis may be associated with defects in peripheral blood polymorphonuclear leukocyte (pmn) function, phagocytosis and chemotaxis were analyzed in 31 otherwise healthy patients and 12 unaffected controls. when compared to controls, no chemotactic defects to 10 nm f-met-leu-phe (fmlp) were detected. in contrast, phagocytosis was significantly impaired (p < 0.001). the mean rates of adhesion and ingestion of opsonized staphylococcus aureus by pmns were 7.1 +/- 1.7 (+/ ... | 1992 | 1333526 |
| biological role of an arginine residue present in a histidine-rich peptide which inhibits hemagglutination of porphyromonas gingivalis. | inhibitory effects of synthetic fragments in histatin 8, having the sequence lys-phe-his-glu-lys-his-his-ser-his-arg-gly-tyr, on hemagglutination by porphyromonas gingivalis 381 were examined. the hemagglutinating activity was reduced much more by the peptide lys-his-his-ser-his-arg-gly-tyr than by the peptides lys-his-his-ser-his and/or lys-phe-his-glu-lys. these results suggest that the arginine residue may have an important role in the inhibition of hemagglutination by p. gingivalis. | 1992 | 1334017 |
| periodontal bone loss in porphyromonas gingivalis-infected specific pathogen-free rats after preinoculation with endogenous streptococcus sanguis. | anaerobic gram-negative bacteria dominate in periodontitis locations, while gram-positive bacteria characterize healthy sites. a well-established gram-positive flora might therefore inhibit the colonization of gram-negative pathogens. the purpose of the present investigation was to examine whether endogenous s. sanguis could prevent, or reduce, periodontal bone loss in rats infected with a virulent p. gingivalis strain. sixty specific pathogen-free wistar rats were divided into 6 groups. doxycyc ... | 1992 | 1334145 |
| sensitization of oral bacteria in biofilms to killing by light from a low-power laser. | biofilms of streptococcus sanguis, porphyromonas gingivalis, fusobacterium nucleatum and actinobacillus actinomycetemcomitans were prepared on the surfaces of agar plates and a number of compounds were screened for their ability to sensitize bacteria in these biofilms to killing by light from a 7.3 mw helium/neon (he/ne) laser. toluidine blue o and methylene blue enabled detectable killing of all four target organisms after exposure to he/ne light for 30 s. aluminium disulphonated phthalocyanine ... | 1992 | 1334649 |
| expression of fc-gamma-riii and fibronectin in peripheral polymorphonuclear neutrophils with increased response to fc stimulation in patients with juvenile periodontitis. | the nature of increased luminol-enhanced chemiluminescence (cl) in peripheral polymorphonuclear neutrophils (pmn) in juvenile periodontitis is of pathophysiological interest and may serve as a model for tissue damage caused by granulocytes. activation of pmns by opsonized staphylococcus aureus was compared with that of actinobacillus actinomycetemcomitans and bacteroides gingivalis, regarded as being more specific for juvenile periodontitis. the cl was higher in the pmns from the patients, indep ... | 1992 | 1335228 |
| inhibition of bone dna and collagen production by surface-associated material from bacteria implicated in the pathology of periodontal disease. | gentle extraction of oral bacteria implicated in the pathogenesis of periodontal disease, actinobacillus actinomycetemcomitans, porphyromonas gingivalis, or eikenella corrodens, with saline removes the extracellular components while leaving the bacteria intact. this readily-solubilized surface-associated material (sam) has been demonstrated to significantly inhibit dna and collagen synthesis by murine calvaria at concentrations as low as 10 ng/ml. dna and collagen synthesis in isolated calvarial ... | 1992 | 1335499 |
| further evidence for a possible role of trypsin-like activity in the adherence of porphyromonas gingivalis. | the present study was undertaken to evaluate a possible correlation between the level of trypsin-like activity and the adherence properties of porphyromonas gingivalis. it was demonstrated that strains with high cell-associated trypsin-like activity attach in higher numbers to human epithelial cells than strains with low levels of trypsin-like activity. to a lesser extent, the same tendency was also noted for the agglutination of human erythrocytes. the ability of p. gingivalis to attach to eryt ... | 1992 | 1335828 |
| bacterial concentration fluorescence immunoassay (bcfia) for the detection of periodontopathogens in plaque. | a bacterial concentration fluorescence immunoassay (bcfia) was developed to rapidly detect periodontopathic bacteria in human plaque samples. the bcfia utilized fluorescent-tagged monoclonal antibodies (mabs) directed against the lipopolysaccharide of selected gram-negative bacteria. microorganisms identified in plaque using the bcfia included actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum. the immunoassay procedure involved com ... | 1992 | 1336047 |
| dna probe and enzyme analysis in periodontal diagnostics. | recent research in periodontology has indicated that a finite number of bacterial species are associated with periodontal disease. this has generated the hypothesis that periodontal disease is essentially an infection due to one or more of the putative periodontopathogens; i.e., the specific plaque hypothesis (sph). this microbial specificity paradigm has already changed the way researchers and some clinicians view periodontal disease. the clinician must heed several cautions signs, however, if ... | 1992 | 1336048 |
| characteristics and utilization of antibody measurements in clinical studies of periodontal disease. | the detection and quantitation of immune responses to infections have long been used as a diagnostic tool in medical infections. recently, increasing evidence has supported that active, specific antibody responses to selected members of the subgingival microbiota are noted in periodontitis patients. this report describes the various specificities of this antibody as they relate to periodontitis classification and prognosis. the functional aspects of the serum antibody have come under increasing ... | 1992 | 1336049 |
| antimicrobial effect of a dental varnish, in vitro. | the effects of a polymer based antimicrobial releasing varnish cervitec were investigated against different gram-positive and gram-negative bacterial strains as well as a yeast using the agar diffusion inhibitory test (adt-test in vitro). as positive controls a 1% chlorhexidine gel and 1% aqueous solution of thymol and a placebo polymer varnish without active agents were employed. the test experimental varnish containing 1% chlorhexidine and 1% thymol showed antimicrobial activity against all gr ... | 1992 | 1336221 |
| demonstration of a bimodal coaggregation reaction between porphyromonas gingivalis and treponema denticola. | this study demonstrates a strong coaggregation reaction between 2 suspected periodontopathogens: porphyromonas gingivalis and treponema denticola. other black-pigmented oral bacterial species tested did not coaggregate with t. denticola. this specific interbacterial aggregation was bimodal, since heating of both cell types was required to completely eliminate the reaction. the coaggregation reaction occurred between ph 4 and 9. under some conditions, arginine and d-galactosamine were effective i ... | 1992 | 1337373 |
| antibacterial efficacy of root canal medicaments. | the antibacterial efficacy of four endodontic medicaments was studied in vitro. calcium hydroxide (calacept), camphorated parachlorophenol (cpcp), cresophene, and 2% iodine-potassium iodide (iki) were tested against four anaerobic microorganisms, streptococcus mutans, peptostreptococcus anaerobius, porphyromonus gingivalis and fusobacterium nucleatum, for 10 or 15 min. iki was effective only against f. nucleatum and p. gingivalis, and the others were effective against all four microorganisms. | 1992 | 1337555 |
| [the value of using serum specific antibody in differential diagnosis of periodontitis]. | in the present study, serum anti-bacteroides gingivalis (bg) and anti-actinobacillus actinomycetemcomitans (aa) antibody titers in rpp, ap patients and healthy subjects were determined by elisa. discriminant analysis for differential diagnosis of rpp, ap and healthy subjects was performed using clinical measurements (pd, al, pii, bi) and serum antibody levels as combined parameters. the results showed significantly different serum antibody levels among the three examined groups. the results also ... | 1992 | 1338038 |
| avidity and titer of immunoglobulin g subclasses to porphyromonas gingivalis in adult periodontitis patients. | the relative avidity and titer of antibodies representing the 4 immunoglobulin g (igg) subclasses (igg1-4) reactive with porphyromonas gingivalis, p. gingivalis-lipopolysaccharide (-lps), streptokinase (sk) and tetanus toxoid (tt) in the sera of patients having adult periodontitis and of healthy controls were measured. patient antibody titers to p. gingivalis and p. gingivalis-lps were found to be significantly elevated for igg, igg1 (no p. gingivalis-lps antibodies) and igg2. the predominant an ... | 1992 | 1338600 |
| evaluation of a non-radioactive dna probe for detecting porphyromonas gingivalis in subgingival specimens. | this study compared the ability of a nonradioactive digoxigenin-labeled dna probe and anaerobic culture to identify subgingival porphyromonas gingivalis. total cellular dna from p. gingivalis atcc 33277t was labeled using the genius kit from boehringer mannheim biochemicals. anaerobic culture was performed using vmga iii transport medium and enriched brucella blood agar. the dna probe could detect as little as 1000 p. gingivalis cells added to supragingival plaque. also, the probe could detect p ... | 1992 | 1338601 |
| expression of porphyromonas gingivalis proteolytic activity in escherichia coli. | porphyromonas gingivalis (formerly bacteroides gingivalis) degrades numerous protein substrates including collagen, fibrinogen, fibronectin, gelatin, casein, immunoglobulins and complement components. in order to clone one or more of these protease genes, a genomic library was constructed with sau3a1 restriction fragments of chromosomal dna from p. gingivalis atcc 33277 ligated into the temperature-regulated vector pcqv2, and expressed in escherichia coli dh5 alpha mcr. the electro-transformants ... | 1992 | 1338602 |
| interaction of porphyromonas gingivalis with gingival epithelial cells maintained in culture. | interactions between porphyromonas gingivalis and gingival epithelial cells were investigated. gingival epithelial cells were cultured from surgically removed gingival tissue. electron microscopy demonstrated adherence of p. gingivalis to the cell membranes and microvilli followed by internalization of the bacteria into the epithelial cell cytoplasm. saliva from healthy and periodontally diseased patients inhibited p. gingivalis association with the epithelial cells. attachment to and penetratio ... | 1992 | 1338603 |
| identity of amino acid sequences of superoxide dismutase purified from both anaerobically maintained and aerated porphyromonas gingivalis. | superoxide dismutase (sod) from anaerobically maintained porphyromonas gingivalis (anaero-sod) has the characteristic of fe-sod, whereas sod from aerated cells (aero-sod) has that of mn-sod. however, both types of aposod can bind either fe or mn. to elucidate the structure relationship between anaero- and aero-sod, we examined the amino acid sequence of aero-sod by direct protein analysis. the amino acid sequence of aero-sod was shown to be identical with that of anaero-sod determined previously ... | 1992 | 1338604 |
| the relationship between polysaccharide antigen and interleukin-1 beta producing activity in porphyromonas gingivalis. | strains of porphyromonas gingivalis were serologically classified into three groups (a, b, and c) by immunodiffusion test using the autoclave extracted antigens. all tested strains had proteolytic activity, but the activity differed from strain to strain, regardless of serogroup. it was found that serogroup a strains had more collagenolytic activity than did serogroup b (p < 0.05), but that the three groups have no remarkable differences in trypsin activity. all autoclave extracted serogroup-spe ... | 1992 | 1338913 |
| putative periodontopathogens in "diseased" and "non-diseased" persons exhibiting poor oral hygiene. | the aim of the study was to assess the occurrence of some putative periodonto-pathogens in "test" and "control" sites in "diseased" and "non-diseased" persons, respectively, from an adult rural kenyan population exhibiting poor oral hygiene and widespread loss of attachment (la). 14 persons (less than 35 years) were assigned to a "diseased" category on the basis of at least 4 sites with la greater than or equal to 4 mm; at least 5 mm la and a pocket greater than or equal to 4 mm interproximally ... | 1992 | 1346265 |
| synthetic peptides analogous to the fimbrillin sequence inhibit adherence of porphyromonas gingivalis. | fimbriae are important in the adherence of many bacterial species to the surfaces they eventually colonize. porphyromonas (bacteroides) gingivalis fimbriae appear to mediate adherence to oral epithelial cells and the pellicle-coated tooth surface. the role and contribution of fimbriae in the binding of p. gingivalis to hydroxyapatite (hap) coated with saliva as a model for the pellicle-coated tooth surface were investigated. 3h-labeled p. gingivalis or the radioiodinated purified fimbriae were i ... | 1992 | 1347762 |
| restriction fragment length polymorphism analysis of the fimbrillin locus, fima, of porphyromonas gingivalis. | with hybridization probes derived from the fimbrial locus of porphyromonas gingivalis strain 381, fima381, restriction fragment length polymorphisms (rflps) were examined at the fimbrillin locus in 39 human and animal strains of this species. the 39 strains were subdivided into nine rflp groups (i-ix) after genomic digests were probed with the internal coding sequence of the fima381 gene. thirty-three strains showed one or more alui fragments of moderate-to-high homology (greater than or equal t ... | 1992 | 1351483 |
| immunization with porphyromonas (bacteroides) gingivalis fimbriae protects against periodontal destruction. | adhesive fimbriae from porphyromonas gingivalis are cell surface structures which may be important in the virulence of this oral pathogen and thus may serve as a critical or target antigen. immunization with highly purified 43-kda fimbrial protein protected against periodontal tissue destruction when tested in the p. gingivalis-infected gnotobiotic rat model. a similarly highly purified 75-kda cell surface component did not provide protection. heat-killed whole-cell and sonicated cell surface ex ... | 1992 | 1351883 |
| periodontal bone loss in mice induced by different periodontopathic organisms. | periodontal bone loss in mice orally inoculated with peptostreptococcus anaerobius, pept. magnus and actinobacillus actinomycetemcomitans was compared to that in sham-inoculated mice. six-to-8-week-old balb/c mice were inoculated with 1 x 10(5), 1 x 10(7) or 1 x 10(9) colony-forming units (c.f.u.) of bacteria in 50 microliters of medium. ten mice received each concentration of bacteria and 10 sham-inoculated mice acted as controls. five mice from each of the groups were killed 6 weeks after inoc ... | 1992 | 1353347 |
| further studies on some physical and biochemical characteristics of asaccharolytic pigmented bacteroides of feline origin. | the ultrastructure of the appendages of 24 strains of asaccharolytic pigmented bacteroides spp. of cats was studied by transmission electron microscopy. all strains examined by thin section showed abundant fimbriae, outer membrane vesicles and capsules. negative staining showed fimbriae which varied from long, fine and wavy in bact. salivosus and cat group 2 to shorter, less abundant and thicker fimbriae in cat strains of bact. gingivalis as well as type strains of porphyromonas gingivalis and p ... | 1992 | 1353758 |
| immunoglobulins in milk from cows immunized with oral strains of actinomyces, prevotella, porphyromonas, and fusobacterium. | immunization of pregnant cows with bacteria leads to the presence of high concentrations of specific antibodies in colostrum and milk. a total of 14 cows was immunized with single strains of heat-killed oral bacteria or pools of strains of actinomyces, porphyromonas, prevotella, and fusobacterium. two cows were treated with adjuvant alone. the mean percentages of igg1, igg2, igm, and iga in all of the milks were 83.8, 3.8, 9.3, and 3.1, respectively. elisa and whole cell agglutination assays dem ... | 1992 | 1354674 |