| an attempt to apply lethal synthesis to the design of chemotherapeutic agents. fluorinated 5 beta-(hydroxyethyl)-4-methylthiazoles. | | 1979 | 106121 |
| interaction of soluble brain adenylate cyclase with manganese. | | 1979 | 106055 |
| semicarbazide-sensitive amine oxidase in rat aorta: a plasma-membrane enzyme [proceedings]. | | 1979 | 93954 |
| differential location of different types of intermediate-sized filaments in various tissues of the chicken embryo. | the location of constitutive proteins of different types of intermediate-sized (about 10 mm) filaments (cytokeratin, vimentin, desmin, brain filament protein) was examined in various tissues of 11--20 day chick embryos, using specific antibodies against the isolated proteins and immunofluorescence microscopy on frozen sections and on isolated serous membrane. the tissues studied which contained epithelia were small intestine, gizzard, esophagus, crop, liver, kidney, thymus, mesenteries, and epid ... | 1979 | 93557 |
| quantitative succinate-dehydrogenase histochemistry. i. a methodological study on mammalian and fish muscle. | in enzyme histochemistry formazan production can be used as a measure for oxidative enzyme activity. the formazan deposits can be measured quantitatively per cell with a scanning and integrating microspectrophotometer. optimal conditions are described for the estimation of histochemical succinate dehydrogenase activity in sections of fish bodymusculature and mouse soleus and plantaris muscle. it is shown that when proper measuring conditions are chosen a ditetrazolium salt (tnbt) can be used in ... | 1979 | 93099 |
| effect of ischaemia on muscle contraction in man [proceedings]. | | 1979 | 92943 |
| effect of radiotherapy on blood lymphocyte population in mammary carcinoma. | | 1976 | 184069 |
| [treatment of experimental staphylococcal endophthalmitis]. | | 1979 | 92092 |
| sexual differentiation and testosterone production. | | 1976 | 184389 |
| spontaneous interictal spiking in the awake kindled rat. | the spontaneous interictal spike (sis) noted in eeg recordings is a signal of the abnormally excitable behavior of neurons in an epileptic focus. this study provides a detailed, quantitative, temporal and spatial profile of sis in the amygdaloid kindled rat. the reproducible nature of the development and decline of sis in kindling provides a useful model for biochemical study of the mechanisms that initiate and regulate sis. | 1979 | 91486 |
| a comparison of the prophylactic and therapeutic effects of poly i:c and endotoxin in mice infected with mengo virus. | we have compared the protective effect in akr mice of poly i:c and bacterial endotoxins against lethal doses of mengo virus. administered intravenously or intraperitoneally, both interferon inducers protected mice to about the same extent from virus challenges of 2-3 ld50's. endotoxin, however, was unable to protect the mice effectively against higher challenge doses of virus. evidence is presented that the level of protection afforded by both inducers is related to the level of circulating inte ... | 1976 | 184902 |
| potentiation of the acth response to metyrapone by l-dopa in the monkey. | the intravenous injection of l-dopa (15 mg/kg) to monkeys (macaca mulatta) failed to alter plasma concentrations of acth and of 11-deoxy-cortisol. when cortisol synthesis was blocked with iv metyrapone, potentiation of acth secretion by l-dopa became apparent. simultaneous injection of l-dopa and metyrapone resulted in a marked increase in plasma acth from 93 +/- 18 pg/ml to 432 +/- 80 pg/ml, whereas plasma 11-deoxycortisol increased from 1.5 +/- 0.2 mug/100 ml to 14.6 +/- 1.0 mug/100 ml 90 min ... | 1976 | 185048 |
| detection of a low-molecular-weight antigen on melanoma cells by a human antiserum in leukocyte-dependent antibody assays. | biochemical characterization of serologically detected human melanoma antigens was undertaken for the development of immunodiagnostic assays in melanoma. an antiserum from a human melanoma patient, which detected melanoma antigens expressed on a large proportion of different melanoma cells, was used in leucocyte-dependent cytotoxic antibody (lda) 51cr-release assays to monitor the purification of melanoma antigens in urea/acetate extracts of lactoperoxidase 125i-labelled melanoma cell membranes. ... | 1979 | 91379 |
| [effect of human antigens on the biological properties of the causative agents of typhoid fever and paratyphoid b]. | | 1979 | 91281 |
| proceedings: facilitation of the tonic vibration reflex in the spinal cat by 5-hydroxytryptophan (5-htp). | | 1975 | 168362 |
| presence of the methylester of 5-carboxymethyl uridine in the wobble position of the anticodon of trnaiii arg from brewer's yeast. | the methylester of 5-carboxymethyluridine (mcm5u), its degradation product 5-carboxymethyluridine (cm5u) and the corresponding nucleotide (cm5up) were isolated from brewer's yeast trnaiii arg or from the dodecanucleotide containing the anticodon. their chromatographic and electrophoretic properties and their uv absorbing spectra were identical to that of the corresponding synthetic compounds. the gas chromatographic behavior and the mass spectrum of mcm5u obtained from trnaiii arg and of a synth ... | 1975 | 167871 |
| effects of several common long chain fatty acids on the properties and lipid composition of the very low density lipoprotein secreted by the perfused rat liver. | 1. livers from normal fed male rats were perfused in vitro with a bloodless medium which contained intially 3% bovine serum albumin and 100 mg% glucose. albumin alone, or myristate (14 : 0), palmitate (16 : 0), palmitoleate (16 : 1), stearate (18 : 0), oleate (18 : 1), or linoleate (18:2) was infused at a constant rate (496 mumol/4 h), as a complex with albumin, during the experiment. 2. the very low density lipoprotein secreted by the liver after infusion of unsaturated fatty acids (16 : 1, 18 ... | 1975 | 167855 |
| functional differentiation of splanchnic a delta fibres in relation to viscerosomatic reflexes. | the splanchnic afferent signal in the myelinated fibre spectrum was analysed in cats and its relationship to viscerosomatic reflex activity was studied. in addition to abeta and adelta components, a further elevation was observed in the sympathetic chain neurogram. according to the conduction rate (15 plus or minus 4 m/sec), it was caused by myelinated fibres 2-3 mu in diameter. the presence of this component at thoracic dorsal root level, or in the sympathetic chain in stimulation of the postga ... | 1975 | 167390 |
| studies of the mechanisms for the induction of in vivo tumor immunity. iv. enhancement of the in vitro generation of secondary cell-mediated cytotoxic response by normal peritoneal macrophages and their culture supernatants. | the effect of macrophages on the induction of the cell-mediated cytotoxicity against a leukemia in a syngeneic system was investigated. the addition of exogenous peritoneal cells from normal c57bl/6 mice enhanced the in vitro secondary cell-mediated cytotoxic response of both spleen and lymph node cells as responding cells against syngeneic fbl-3 leukemia. peritoneal phagocytic macrophages seemed to be responsible for the enhancement. no inhibitory effect was demonstrated by the addition of peri ... | 1979 | 156217 |
| effect of human immunoglobulin preparations on fc rosette formation between anti-d-coated erythrocytes and lymphocytes. | human immunoglobulin (ig) preparations were tested for their inhibitory effect on fc rosette formation between anti-d-coated human erythrocytes and lymphocytes, as compared to their complement activating capacity. both of the two biological activities ascribed to the sites in the fc portion of the igg molecules were found to be reduced in the pepsin-treated, as well as in the s-sulfonated ig preparations, as compared to the activities of the normal human ig preparation. in the plasmin-treated ig ... | 1978 | 150144 |
| purification by affinity chromatography, properties and crystallisation of phosphofructokinase from thermophilic micro-organisms. | | 1975 | 124670 |
| clinical and microbiologic surveillance of neonatal staphylococcal disease. relationship to hexachlorophene whole-body bathing. | neonatal staphylococcal disease occurred in the nurseries of a university hospital three weeks after discontinuation of routine, daily, whole-body bathing of newborns with hexachlorophene. of 16 infants who had clinical manifestations within a two-week period, 11 cases were confirmed bacteriologically. shortly after onset of the outbreak, daily clinical and microbiologic surveillance and control measures on all infants and personnel were inaugurated. clinical surveillance after the outbreak did ... | 1975 | 123704 |
| bacterial gene into monkey cells. | | 1978 | 210401 |
| [radioautographic identification of dopaminergic axons in the rat cns. in vitro marking with tritiated dopamine or noradenaline]. | a new radioautographic technique for the visualization of central dopaminergic fibers is described. this technique is based upon in vitro uptake of triatiated norepinephrine or tritiated dopamine in the presence of monoamine oxidase inhibitor and specific uptake inhibitor in noradrenergic fibers. after a short step in glutaraldehyde, the use of osmium vapours as a fixative, instead of osmic acid immersion, allows a satisfactory retention of triatiated amines within dopaminergic fibers. | 1979 | 120791 |
| comparison of the 10 000 and 43 000 dalton polypeptide populations isolated from the water soluble and insoluble fractions of human cataractous lenses. | | 1979 | 118040 |
| persistence of ketosis despite suppression of lipolysis by 16,16-dimethylprostaglandin e2 in experimental diabetic ketoacidosis in rats. | | 1979 | 113273 |
| [determination of the localization of the kcpa gene on the chromosome of e. coli o124. i. use of the type f' plasmids of varying length]. | the loss of virulence was observed in some of the transconjugates of e. coli o124 in the process of the transfer of the plasmids differing in length: f' 200 ps, f'x 573 and f'x 363, carrying the genes of the chromosome area lac--pur e. the genetic determinants contributing to the ability of e. coli o124 to produce keratoconjunctivitis seem to be localized near the lactose marker, and not near e as in sh. flexneri. in e. coli o124 these genetic determinants are probably localized on both sides of ... | 1979 | 373340 |
| somatic cell genetic analysis of transgenome integration. | the site of association of the human transgenome and host murine chromosomes was determined in several subclones of a stable human/mouse transformed cell line. chromosomes were transferred from each of three transformed subclones into chinese hamster recipient cells, and selection was applied for the expression of human transgenome-encoded hprt. a series of trispecific microcell hybrids was isolated and characterized for each subclone. evidence is presented that, within a given transformed subc ... | 1979 | 545718 |
| experimental and postexperimental response to anteriorly directed extraoral force in young macaca nemestrina. | an anteriorly directed extraoral force was applied to the maxillas of four healthy young m. nemestrina monkeys. experimental, retention, and postretention alterations were evaluated through a combination of cephalometric, histologic, and gross techniques. in view of the results of the present study, the following conclusions can be made: 1. skeletal remodeling occurs in all circummaxillary sutures following the application of an anteriorly directed extraoral force to the maxilla. the amount of r ... | 1979 | 107808 |
| the pathway of formation of acetate and succinate from pyruvate by bacteroides succinogenes. | bacteroides succinogenes produces acetate and succinate as major products of carbohydrate fermentation. an investigation of the enzymes involved indicated that pyruvate is oxidized by a flavin-dependent pyruvate cleavage enzyme to acetyl-coa and co2. active co2 exchange is associated with the pyruvate oxidation system. reduction of flavin nucleotides is coash-dependent and does not require ferredoxin. acetyl-coa is further metabolized via acetyl phosphate to acetate and atp. reduced flavin nucle ... | 1978 | 678020 |
| sarcocystinae: nomina dubia and available names. | examination of the original descriptions of the species of sarcocystis in cattle, sheep, and swine, and of isosporid oocysts shed sporulated by dogs, cats, man, and other carnivores, has shown that it is not possible in most instances to identify unambiguously recently recognized taxa. the original descriptions are insufficient, and because no type specimens exist, could apply to two or more of the presently recognized taxa. we consider the following nomina dubia: sarcocystis hirsuta s. miescher ... | 1979 | 107666 |
| an unstable donor-recipient dna complex in transformation of bacillus subtilis. | in re-extracted dna obtained shortly after uptake of transforming dna by bacillus subtilis, increased amounts of donor dna radioactivity banding at the position of donor-recipient dna complex (drc) are observed in cscl gradients, if the cells are irradiated with high doses of uv prior to reextraction of the dna. qualitatively, the same phenomenon is observed if lysates of transforming cells are irradiated. uv-irradiation of lysates of competent cells to which single-stranded dna is added after l ... | 1978 | 106232 |
| interaction of hunger and thirst in rats with lesions of the preoptic area. | | 1975 | 1135317 |
| depression in the excitability of relay cells of lateral geniculate nucleus following saccadic eye movements in the cat. | 1. the excitability of relay cells of the lateral geniculate nucleus during a saccadic eye movement was studied in alert cats. excitability was assessed by the firing probability of the cells in response to electrical stimulation of the optic chiasm. modifications in the excitability were evaluated during the period following eye movements, by triggering a stimulator from potential shifts in electro-oculogram and altering delays in the stimulus pulse. 2. the cells were classified into s and t ce ... | 1975 | 168363 |
| ia antigens in serum during different murine infections. | there exists in the mouse a family of i-region-controlled (ia) antigens which carry carbohydrate-defined determinants. these antigens appear in serum as glycolipids and seem to be actively secreted by antigen-activated t-cells. this paper describes the ability of selected viral, bacterial, and protozoal infections of mice to markedly alter the serum levels of these ia antigens. all the infectious agents examined induced substantial augmentation or suppression of serum ia concentrations or both. ... | 1979 | 94905 |
| human cytomegalovirus. iii. virus-induced dna polymerase. | infection of wi-38 human fibroblasts with human cytomegalovirus (cmv) led to the stimulation of host cell dna polymerase synthesis and induction of a novel virus-specific dna polymerase. this cytomegalovirus-induced dna polymerase was purified and separated from host cell enzymes by deae-cellulose and phosphocellulose column chromatographies. it can be distinguished from host cell enzymes by chromatographic behavior, template primer specificity, sedimentation property, and the requirement of sal ... | 1975 | 168404 |
| conditional lethal mutants of vesicular stomatitis virus. | | 1975 | 169102 |
| structure of the mannoheptose-containing pentaglycosyldiacylglycerol from acholeplasma modicum. | | 1976 | 182247 |
| lithogenic bile after conjugated estrogen. | reference is made to a previous paper by bennion et al. describing t he effects of contraceptive steroids on the lipid composition of gall bladder bile. a similar rise in cholesterol saturation of hepatic bile was observed after giving conjugated equine estrogens (.5 mg/kg) to 7 fa sting patients with indwelling t-tubes. liver function tests were norma l and bile culture was negative. 1 ml of hepatic bile was collected aft er 6, 12, and 24 hours for lipid determinations. the administration o ... | 1976 | 184386 |
| particulate carbonic anhydrase in homogenates of human kidney. | about 2% of human kidney carbonic anhydrase (carbonate hydro-lyase, ec 4.2.1.1) has been found in particulate fractions. its distribution in the particulate fractions obtained by differential centrifugation suggests that it may be concentrated in the brush border. the particulate enzyme is like red cell carbonic anhydrace c in its susceptibility to inhibition by anions. particulate carbonic anhydrase is firmly bound to the membrane and is not released by incubation at ph 10.6 and 37 degrees c or ... | 1976 | 184830 |
| degradation of maize stem by two rumen fungal species, piromyces communis and caecomyces communis, in pure cultures or in association with cellulolytic bacteria. | two species of rumen fungi, piromyces (piromonas) communis fl and caecomyces (sphaeromonas) communis fg10, were cultured alone or in association with the cellulolytic bacteria ruminococcus flavefaciens or fibrobacter succinogenes on maize stem. a kinetic study of the degradation of the substrate was then made. after 48 h of culture, all non-lignified tissues observed by scanning electron microscopy disappeared with p communis and degradation was as complete as that observed in the rumen. in cont ... | 1992 | 1418394 |
| liver monoamine oxidase (mao) in liver homogenate of mice infected with schistosoma mansoni and effect of certain therapeutic agents. | mao activity in liver homogenate of mice infected with schistosoma mansoni was determined from the second till the 14th week following infection. significant diminution of mao activity was noticed starting from the sixth week following infection, reaching its lowest value at the eighth week, obviously denoting progress of hepatic fibrosis. treatment of the infected, animals with four different antischistosomal agents, tarter emetic, stibophen, niridazole and hycanthone, resulted in an improvemen ... | 1977 | 203063 |
| rabbit mammary prolactin receptors. demonstration of a late puerperal increase in affinity. | crude receptor preparations of rabbit mammary gland were made by differential centrifugation and reacted with lactoperoxidase-iodinated ovine prolactin (oprl) in order to determine their binding characteristics. receptors prepared from the mammary glands of animals less than 4 days postpartum bound oprl with high affinity (ka = 3.50 x 10(9) m-1), in good agreement with previous results of other investigators. the binding capacity of these preparations was 107 +/- 16.3 fmol/mg of protein. in cont ... | 1978 | 203595 |
| an adenosine 3':5'-monophosphate-adenosine binding protein from mouse liver. a study on its interaction with adenosine 3':5'-monophosphate and adenosine. | | 1978 | 203599 |
| [studies of the contamination of coastal waters of the baltic sea by viruses]. | | 1977 | 204116 |
| phosphoprotein phosphatase in bovine tracheal smooth muscle. multiple fractions and multiple substrates. | phosphoprotein phosphatase (phosphoprotein phosphohydrolase, ec 3.1.3.16) from bovine tracheal smooth muscle extracts was isolated and its activity determined using two [32p]phosphorylated proteins as substrates, i.e. phosphorylated histone (h-p) and a phosphorylated muscle specific substrate protein (ms-p) for the tracheal smooth muscle protein kinase. the enzyme was purified by the use of deae-cellulose followed by a two stage chromatography on a histone-sepharose affinity column. elution from ... | 1978 | 204354 |
| studies on neutralization of varicella-zoster virus and serological follow-up of cases of varicella and zoster. | | 1978 | 208504 |
| [production and immunologic study of anticytochrome oxidase sera to dog heart and skeletal muscle]. | | 1978 | 210051 |
| regulation by patterned preganglionic neural activity of transmitter stores in a sympathetic ganglion. | 1. the effect of patterned preganglionic stimulation on acetylcholine (ach) stores was studied in the superior cervical ganglion of the cat. 2. patterns of stimulation based on 0.5 and 1.0 s trains at frequencies from 10 to 80/s and intervals between trains of 2-180 s increased ach stores, over periods of 1-3 h stimulation, to the same extent (30-70%) as found earlier to result from continuous stimulation. 3. if the mean frequency of the overall pattern was greater than 5/s the increase in store ... | 1978 | 211230 |
| time-related decrease of substance p and cgrp in central and peripheral projections of sensory neurones in mycobacterium leprae infected nude mice: a model for lepromatous leprosy in man. | we have previously shown the depletion of cutaneous calcitonin gene-related peptide (cgrp)- and substance p-containing nerves in human leprosy. the aims of this study were to investigate the temporal effects of leprosy on nerves in skin and spinal cord. tissues were taken from nude mice, 6 and 12 months after inoculation of mycobacterium leprae into the hind footpads, and from age-matched controls. sections were immunostained with antisera to substance p or cgrp. after 6 months of infection, sub ... | 1990 | 1694240 |
| studies on cytochrome c oxidase, iii. relationship of cytochrome oxidase subunits to electron carriers of photophosphorylation. | | 1978 | 213364 |
| an inducible proline transport system in candida albicans. | 1. when candida albicans cells were preincubated with proline or grown in the presence of proline as the sole nitrogen source they exhibited a rapid increase in the influx of proline (the inducible transport system). 2. the induction appeared to be specific for proline and also demonstrated in other candida species. 3. both the inducible and constitutive proline uptake systems exhibited similar characteristic features. 4. the nature of the inducer for proline uptake in c. albicans appeared to be ... | 1979 | 383150 |
| role of glia in k+ and ph homeostasis in the neonatal rat spinal cord. | stimulation-evoked transient changes in extracellular potassium ([k+]e) and ph (phe) were studied in the neonatal rat spinal cords isolated from 3-13-day-old pups. in unstimulated pups the [k+]e baseline was elevated and phe was more acid than that in ringer's solution (3.5 mm k+, ph 7.3-7.35). the [k+]e and phe in 3-6-day-old pups was 3.91 +/- 0.12 mm and phe 7.19 +/- 0.01, respectively, while in 10-13-day-old pups it was 4.35 +/- 0.15 mm and 7.11 +/- 0.01, respectively. the [k+]e changes evoke ... | 1991 | 1828787 |
| adhesion of bacteroides succinogenes in pure culture and in the presence of ruminococcus flavefaciens to cell walls in leaves of perennial ryegrass (lolium perenne). | bacteroides succinogenes and ruminococcus flavefaciens are two of the most important cellulolytic bacteria in the rumen. adhesion of b. succinogenes in pure culture, and in mixed culture with r. flavefaciens, to the various types of cell walls in sections of perennial ryegrass (lolium perenne l. cultivar s24) leaves was examined by transmission and scanning electron microscopy. b. succinogenes adhered to the cut edges of most plant cell walls except those of the meta- and protoxylem. it also adh ... | 1978 | 567035 |
| some effects of arsenic on the rumen microflora; an in vitro study. | the rate of fermentation of the rumen microflora was inhibited almost 30% by 5 micrograms/ml of arsenic added in the form of arsenite, although 304 micrograms/ml was required to cause 50% inhibition. arsenate was less inhibitory. the rate of fermentation of a separated bacterial fraction was inhibited 37% by 1 microgram of arsenite per millilitre, whereas 100 micrograms/ml had little effect on the fermentation of a separated protozoal fraction. similar results were obtained for arsenate. both fr ... | 1978 | 754875 |
| ampicillin-resistant hemophilus sp. | | 1976 | 1082793 |
| dap-decarboxylase activity and lysine production by rumen bacteria. | the last step of pathway of lysine biosynthesis by rumen bacteria was tested. the first measurements of dap-decarboxylase activity and of lysine production by megasphera elsdenii, selenomonas ruminantium, clostridium spp., butyrivibrio fibrisolvens and bacteroides succinogenes as well as the first attempts to increase the lysine production by ruminal streptococci by mutation are described. the highest values were measured in selenomonas ruminantium (dap-decarboxylase activity = 146 micrograms da ... | 1992 | 1295484 |
| structure of holo-chaperonin studied with electron microscopy. oligomeric cpn10 on top of two layers of cpn60 rings with two stripes each. | a structural model of holo-chaperonin, known as a protein-folding control protein comprising 60 kda (cpn60) and 10 kda polypeptides (cpn10), is proposed based on the electron microscopic images of holo-chaperonin from thermus thermophilus and cpn60 from paracoccus denitrificans. isolated paracoccus cpn60 shows very similar images to those of escherichia coli tetradecameric cpn60, a seven-membered ring in the top view and a rectangular shape with four stripes in the side view. however, a small nu ... | 1992 | 1347504 |
| presynaptic dopaminergic inhibition of the spinal reflex in rats. | dopaminergic influence on spinal monosynaptic transmission was examined in rats. monosynaptic mass reflex (mmr) was recorded from the ventral root l6 following supramaximal stimulation (0.2 hz; 0.1 ms) to the ipsilateral dorsal root l6 in spinalized rat under pentobarbitone sodium (40 mg/kg, i.p.) anaesthesia. mmr was inhibited by intravenous administration of the dopaminergic agonist, apomorphine (50-200 ug/kg) in a dose-dependent manner. the attenuatory effect of apomorphine (200 ug/kg i.v.) o ... | 1992 | 1352179 |
| deoxyribonuclease activity in rumen bacteria. | deoxyribonuclease activity was surveyed in 22 strains belonging to 12 species of rumen bacteria, with lambda bacteriophage dna as substrate. activity was readily detected in broken cell preparations from 15 of these strains. particularly high levels of activity were present in cells and culture supernatant of all 5 strains of bacteroides succinogenes, and 2 out of 6 strains of bacteroides ruminicola, examined. | 1990 | 1366671 |
| effects of glycerol on the growth, adhesion, and cellulolytic activity of rumen cellulolytic bacteria and anaerobic fungi. | the effect of glycerol on the growth, adhesion, and cellulolytic activity of two rumen cellulolytic bacterial species, ruminococcus flavefaciens and fibrobacter succinogenes subsp. succinogenes, and of an anaerobic fungal species, neocallimastix frontalis, was studied. at low concentrations (0.1-1%), glycerol had no effect on the growth, adhesion, and cellulolytic activity of the two bacterial species. however, at a concentration of 5%, it greatly inhibited their growth and cellulolytic activity ... | 1992 | 1368974 |
| extracellular beta-galactosidase activity of a fibrobacter succinogenes s85 mutant able to catabolize lactose. | fibrobacter succinogenes s85 is unable to grow with lactose as the source of carbohydrate, although it does exhibit low beta-galactosidase (ec 3.2.1.23) activity. spontaneous mutants of strain s85 able to grow on lactose were isolated after spreading cells on a chemically defined agar medium with lactose as the carbohydrate source. a lactose-catabolizing isolate, designated l2, exhibited a sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein profile and an immunoblot profile with po ... | 1990 | 2128006 |
| two beta-glucosidase activities in fibrobacter succinogenes s85. | few bacteria are capable of degrading crystalline cellulose but there is considerable interest in the properties of enzyme systems with this capability. in the bovine and ovine rumen the principal cellulolytic bacterium is fibrobacter (formerly bacteroides) succinogenes. the cellulase system of this organism is composed of multiple enzyme components, including a constitutive and cell-associated beta-glucosidase active against cellobiose. the properties of the beta-glucosidase activity have been ... | 1992 | 1399917 |
| alterations of acetylcholine enzymes in neuroblastoma cells persistently infected with lymphocytic choriomeningitis virus. | | 1977 | 193868 |
| cyclophosphamide induced sensitivity against avian rna myeloblastosis virus in age-resistant hosts. | the effect of cyclophosphamide administration on survival of 4- to 7-week-old chickens as well as on induction of leukemia after avian myeloblastosis virus (amv) injection was studied. the drug treatment alone did not cause any neoplastic effect in the birds during 4 months of observation. immediate application of amv to cyclophosphamide-pretreated age-resistant chickens induced acute myeloblastic leukemia in about 80 per cent of test animals. the sensitivity of chickens against amv, induced by ... | 1977 | 201874 |
| localization of bacterial antigens in calves inoculated orally with ruminal bacteroides succinogenes and selenomonas ruminantium. | localization of bacteria and bacterial materials was investigated in calves inoculated orally with live organisms of both bacteroides succinogenes and selenomonas ruminantium by a immunohistological method using rabbit antiserum against the outer membrane of those organisms and by a scanning electron microscope. the intact organisms of both inoculated bacterial species were detected on the rumen wall and in the lamina propria of the forestomach, and s. ruminantium also in the lymph nodes associa ... | 1990 | 2202844 |
| [use of glucose and cellobiose by 3 strains of fibrobacter succinogenes]. | f succinogenes strains s85, 128 and 095 were compared with respect to their growth using glucose and/or cellobiose as the carbon and energy substrate(s) and their capacities to degrade cellulose. the growth rate of f succinogenes strain s85 was the same using glucose or cellobiose, whereas the growth rates of strains 128 and 095 were about thrice the rate when using cellobiose. strain s85 could simultaneously use glucose and cellobiose, while strains 128 and 095 tended to use preferentially gluc ... | 1990 | 2206331 |
| a fluorescence enhancement assay of cell fusion. | two probes were synthesized which consist of fluorescent molecules conjugated to saturated hydrocarbon chains, 18 carbons long, to ensure their localization into cellular membranes. there is an overlap between the emission spectrum of one probe (donor) and the absorption spectrum of the other probe (acceptor). by the use of appropriate wavelengths it is possible to specifically excite the donor probe and record the fluorescence of the acceptor probe. two cell populations, each labelled with one ... | 1977 | 202606 |
| purification, characterization, and mode of action of endoxylanases 1 and 2 from fibrobacter succinogenes s85. | two different endoxylanases (1,4-beta-d-xylan xylanohydrolases, ec 3.2.1.8), designated 1 and 2, have been purified by column chromatography to apparent homogeneity from the nonsedimentable extracellular culture fluid of the strictly anaerobic, ruminal bacterium fibrobacter succinogenes s85 grown on crystalline cellulose. endoxylanases 1 and 2 were shown to be basic proteins of 53.7 and 66.0 kda, respectively, with different ph and temperature optima, as well as different substrate hydrolysis ch ... | 1992 | 1539970 |
| futile cycling of glycogen in fibrobacter succinogenes as shown by in situ 1h-nmr and 13c-nmr investigation. | glycogen was synthesized during all the growth phases in the rumen anaerobic cellulolytic bacterium fibrobacter succinogenes. glycogen synthesis and degradation were monitored using in situ 13c and 1h-nmr spectroscopy in resting cells of f. succinogenes. the cells were incubated at 37 degrees c under anaerobic conditions with [1-13c]glucose and [2-13c]glucose. 1h-nmr spectra were used to quantify enrichment by 13c of metabolism products. glucose was utilized for energy requirements of the bacter ... | 1992 | 1628646 |
| enzymes associated with metabolism of xylose and other pentoses by prevotella (bacteroides) ruminicola strains, selenomonas ruminantium d, and fibrobacter succinogenes s85. | prevotella (bacteroides) ruminicola strains b(1)4 and s23 and selenomonas ruminantium strain d used xylose as the sole source of carbohydrate for growth, whereas fibrobacter succinogenes was unable to metabolize xylose. prevotella ruminicola strain b(1)4 exhibited transport activity for xylose. in contrast, f. succinogenes lacked typical xylose uptake activity but did exhibit low binding potential for the sugar. prevotella ruminicola strains b(1)4 and s23 as well as s. ruminantium d showed low x ... | 1992 | 1643581 |
| type ii dna restriction-modification system and an endonuclease from the ruminal bacterium fibrobacter succinogenes s85. | fibrobacter succinogenes is an important cellulolytic bacterium found in the rumen and cecum of herbivores. numerous attempts to introduce foreign dna into f. succinogenes s85 have failed, suggesting the presence of genetic barriers in this organism. results from this study clearly demonstrate that f. succinogenes s85 possesses a type ii restriction endonuclease, fsui, which recognizes the sequence 5'-gg(a/t)cc-3'. analysis of the restriction products on sequencing gels showed that fsui cleaves ... | 1992 | 1644754 |
| degradation of cellulose and forage fiber fractions by ruminal cellulolytic bacteria alone and in coculture with phenolic monomer-degrading bacteria. | we hypothesized that bacterial species capable of metabolizing phenolic monomers may act as catalysts for forage fiber breakdown by increasing microbial access to cell wall polysaccharides. ruminal cellulolytic bacteria alone and in combination with phenolic-degrading bacteria were examined for differences in their ability to degrade fiber fractions of alfalfa or bromegrass. electron micrographs of fibrobacter succinogenes s85 cultured in combination with the ruminal phenolic-degrading organisms ... | 1991 | 1667013 |
| fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. | fluorescent-dye-conjugated oligonucleotides were used to classify 14 fibrobacter strains by fluorescence microscopy. on the basis of partial 16s rrna sequences of six fibrobacter strains, four hybridization probes were designed to discriminate between the species fibrobacter succinogenes and fibrobacter intestinalis and to identify f. succinogenes subsp. succinogenes. after in situ hybridization to whole cells of the six sequenced strains, epifluorescence microscopy confirmed probe specificity. ... | 1990 | 1688842 |
| antigenic nature of the chloride-stimulated cellobiosidase and other cellulases of fibrobacter succinogenes subsp. succinogenes s85 and related fresh isolates. | polyclonal and monoclonal antibodies to the cl-stimulated cellobiosidase of fibrobacter succinogenes subsp. succinogenes s85 reacted with numerous proteins of both higher and lower molecular weights from f. succinogenes subsp. succinogenes s85, but not with escherichia coli proteins, and only one protein each from butyrivibrio fibrisolvens and ruminococcus albus. different profiles were observed for western blots (immunoblots) of peptide digests of both the purified enzyme from f. succinogenes a ... | 1990 | 1692677 |
| endafs, a novel family e endoglucanase gene from fibrobacter succinogenes ar1. | the complete nucleotide sequence of endafs, an endoglucanase gene isolated from the ruminal anaerobe fibrobacter succinogenes ar1, was determined. endafs encodes two overlapping open reading frames (orf1 and orf2), and it was proposed that a -1 ribosomal frameshift was required to allow contiguous synthesis of a 453-amino-acid endoglucanase. a proline- and threonine-rich region at the c terminus of orf1 and rare codons for arginine and threonine were coincident with the proposed frameshift site. ... | 1991 | 1708767 |
| molecular cloning and expression in escherichia coli of a cellodextrinase gene from bacteroides succinogenes s85. | a dna fragment coding for a cellodextrinase of bacteroides succinogenes s85 was isolated by screening of a pbr322 gene library in escherichia coli hb101. of 100,000 colonies screened on a complex medium with methylumbelliferyl-beta-d-cellobioside as the indicator substrate, two cellodextrinase-positive clones (cb1 and cb2) were isolated. the dna inserts from the two recombinant plasmids were 7.7 kilobase pairs in size and had similar restriction maps. after subcloning from pcb2, a 2.5-kilobase-p ... | 1989 | 2650617 |
| structure of the clostridium thermocellum gene licb and the encoded beta-1,3-1,4-glucanase. a catalytic region homologous to bacillus lichenases joined to the reiterated domain of clostridial cellulases. | the nucleotide sequence of the clostridium thermocellum gene licb, coding for a thermoactive beta-1,3-1,4-glucanase, has been determined. the gene is located downstream, but in opposite orientation to the beta-glucosidase gene bgla. a coding region of 1002 bp is flanked by canonical promoter and transcription terminator sequences. the primary translation product of the licb gene has a predicted molecular mass of 37,896 da. the protein sequence can be divided into several discrete segments: an n- ... | 1992 | 1740123 |
| development of the rumen digestive functions in lambs placed in a sterile isolator a few days after birth. | the development of the rumen digestive functions was studied in lambs placed in sterile isolators at 1, 4, 8 or 9 days of age to define the role of the bacterial species that colonize the rumen just after birth. the values of the main rumen digestive parameters (ph, concentrations of volatile fatty acid, ammonia, lactic acid) in these lambs were close to those observed in conventional controls. likewise, the digestive utilisation of the dry matter and starch was comparable in isolated and contro ... | 1991 | 1768310 |
| cloning of a xylanase gene from fibrobacter succinogenes 135 and its expression in escherichia coli. | a genomic library consisting of 4- to 7-kb ecori dna fragments from fibrobacter succinogenes 135 was constructed using a phage vector, lambda gtwes lambda b, and escherichia coli ed8654 as the host bacterium. two positive plaques, designated lambda fsx101 and lambda fsx102, were identified. the inserts were 10.5 and 9.8 kb, respectively. a 2.3-kb ecori fragment that was subcloned from lambda fsx101 into pbr322 also showed xylanase activity. southern blot analysis showed that the cloned ecori fra ... | 1991 | 1913358 |
| molecular cloning, expression, and characterization of endoglucanase genes from fibrobacter succinogenes ar1. | a cosmid gene library was constructed in escherichia coli from genomic dna isolated from the ruminal anaerobe fibrobacter succinogenes ar1. clones were screened on carboxymethyl cellulose, and 8 colonies that produced large clearing zones and 25 colonies that produced small clearing zones were identified. southern blot hybridization revealed the existence of at least three separate genes encoding cellulase activity. prc093, which is representative of cosmid clones that produce large clearing zon ... | 1991 | 2014986 |
| the involvement of transcriptional read-through from internal promoters in the expression of a novel endoglucanase gene fsenda, from fibrobacter succinogenes ar1. | two distinct mrna transcripts were synthesized in escherichia coli during expression of fsenda, an endoglucanase gene from fibrobacter succinogenes ar1. expression of fsenda required a ribosomal frameshift between open reading frame 1 (orf1) and orf2 to allow contiguous translation of a 453 amino acid protein (1). the primary transcript initiated upstream of orf1 and the secondary transcript from within orf1. both transcripts terminated downstream of orf2 and termination was essential for endogl ... | 1991 | 2027774 |
| the hydrolysis of lucerne cell-wall monosaccharide components by monocultures or pair combinations of defined ruminal bacteria. | the defined ruminal bacterial strains fibrobacter succinogenes s85, ruminococcus flavefaciens fd1, ruminococcus albus 7, butyrivibrio fibrisolvens d1, and bacteroides ruminicola ga33 were grown, in monocultures or as combinations of pair strains, on isolated lucerne cell-walls (cw) as the sole carbohydrate substrate. fibrobacter succinogenes s85 was the dominant strain determining extent of cw hydrolysis in all combinations with s85. the hydrolysis of cellulose, xylan, hemicellulose side-sugars, ... | 1991 | 2030098 |
| active enhancement of tissue allografts with antilymphocyte serum and bone marrow. | | 1975 | 242206 |
| [surface changes of the pulmonary valve following pulmonary valve xenotransplantation in the descending aorta. an experimental study in dogs]. | upon implantation in the descending aorta, glutaraldehyde-conditioned pulmonary valves (n = 46) are immediately covered by a protein layer, followed by a fibrinous layer. two to thirty minutes later platelets, wbcs, macrophages, and microthrombi are incorporated into the valve's surface. after 3 weeks the first collagen fibers appear on the surface and slowly build a collagen layer that grows toward the free edge. these changes are very similar to the surface changes of bioprostheses implanted i ... | 1979 | 110559 |
| assessment of abdominal fat deposition in female cynomolgus monkeys. | computed tomography (ct) was used to assess abdominal fat distribution in adult, female cynomolgus macaques (macaca fascicularis). the technique used was similar to that applied in human beings and allowed intra-abdominal, subcutaneous and total abdominal fat to be quantitated in 1 cm thick sections. correlations between single scans and the average of scans at several levels ranged from r = 0.96 to r = 1.00, indicating that a single scan is representative of abdominal fat distribution. signific ... | 1991 | 2045214 |
| cellobiose uptake by the cellulolytic ruminal anaerobe fibrobacter (bacteroides) succinogenes. | cellobiose transport by the cellulolytic ruminal anaerobe fibrobacter (bacteroides) succinogenes was measured using randomly tritiated cellobiose. when assayed at the same concentration (1 mm), total cellobiose uptake was one-fourth to one-third that of total glucose uptake. the abilities of f. succinogenes to transport cellobiose or glucose were not affected by the sugar on which the cells were grown. aspects of the simultaneous transport of [14c(u)]glucose and [3h(g)]cellobiose, the failure of ... | 1991 | 2059920 |
| purification and properties of an acetylxylan esterase from fibrobacter succinogenes s85. | an acetylxylan esterase (ec 3.1.1.6) was purified to apparent homogeneity from the nonsedimentable extracellular culture fluid of fibrobacter succinogenes s85 grown on cellulose. this enzyme had an apparent molecular mass of 55 kda and an isoelectric point of 4.0. the temperature and ph optima were 45 degrees c and 7.0, respectively. the apparent km and vmax were 2.7 mm and 9,100 u/mg, respectively, for the hydrolysis of alpha-naphthyl acetate. the enzyme cleaved acetyl residues from birchwood a ... | 1990 | 2082827 |
| serological and morphological observation on the outer membrane of bacteroides succinogenes. | | 1990 | 2107350 |
| site-specific rate constants for iron removal from diferric transferrin by nitrilotris(methylenephosphonic acid) and pyrophosphate. | the kinetics of iron removal from human serum diferric transferrin by nitrilotris(methylenephosphonic acid) (ntp) and pyrophosphate (ppi) have been studied in 0.1 m, ph 7.4, n-(2-hydroxyethyl)piperazine-n'-2-ethanesulfonate buffer at 25 degrees c using urea/polyacrylamide gel electrophoresis. the four microscopic rate constants required for a complete description of iron removal from the two transferrin metal-binding sites have been measured at 100 mm concentrations of ntp and ppi. there is very ... | 1990 | 2168158 |
| use of dna probes to monitor nutritional effects on ruminal prokaryotes and fibrobacter succinogenes s85. | we used dna probes to study dietary effects on the prokaryotic population in the rumen. procedures used to isolate and quantify prokaryotic 16s ribosomal rna (rrna) from the rumen using universal and species-specific dna probes were evaluated. in this experiment, three ruminally fistulated steers were fed orchard-grass hay, and ruminal digesta were collected at 0, 3, and 9 h after offering hay (0800). samples of ruminal digesta were taken from the interior portion of the digesta mat and from the ... | 1992 | 1374753 |
| [determination of intracellular concentration of h+, na+, k+ and atp in bacteroides succinogenes adapted to monensin]. | the internal concentrations of the cations h+, na+, k+ and the intracellular atp concentration were measured on b succinogenes adapted to the presence of the ionophore monensin (0.5 microm). adapted bacteria were still able to regulate the intracellular concentrations of na+ and k+, but their internal ph and atp concentration were lower than in control bacteria. | 1990 | 2169758 |
| ultrastructure and biosynthetic activity of polyploid atrial myocytes in patients with mitral valve disease. | biopsies of right auricle of human heart have been obtained during open heart surgery from 6 patients aged 23 to 49. the dna and total protein content have been determined in isolated myocytes by two-wavelength scanning cytophotometry after double staining: feulgen and naphthol yellow s. in all the biopsies predominant are polyploid hypertrophied myocytes. both hypertrophied non-degenerating cells and cells with different extent of degenerative changes, primarily of myofibrils and membranes, are ... | 1992 | 1533947 |
| dna sequence of a fibrobacter succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-d-glucan 4-glucanohydrolase) gene. | the dna sequence of a mixed-linkage beta-glucanase (1,3-1,4-beta-d-glucan 4-glucanohydrolase [ec 3.2.1.73]) gene from fibrobacter succinogenes cloned in escherichia coli was determined. the general features of this gene are very similar to the consensus features for other gram-negative bacterial genes. the gene product was processed for export in e. coli. there is a high level of sequence homology between the structure of this glucanase and the structure of a mixed-linkage beta-glucanase from ba ... | 1990 | 2193918 |
| alpha-adrenoceptor responsiveness in the aged rat. | the effects of ageing on vascular alpha 1- and alpha 2-adrenoceptors were examined using anaesthetised and pithed young (3-7 months) and old (21-24 months) sprague-dawley rats. in pithed animals, the pressor and cardioinhibitory effects of the alpha 2-adrenoceptor agonist xylazine were significantly reduced in old animals (8- and 6-fold shift), but the pressor effects of the alpha 1-adrenoceptor agonist amidephrine were not significantly altered by ageing. in anaesthetised rats, the pressor resp ... | 1986 | 3019712 |
| detection of bovine antibodies to the outer membrane of ruminal bacteroides succinogenes by enzyme-linked immunosorbent assay (elisa). | the enzyme-linked immunosorbent assay (elisa) was used to detect bovine serum antibodies directed to the outer membrane antigen of a ruminal bacteria, bacteroides succinogenes. the outer membrane antigen of b. succinogenes was highly reactive against homologous antiserum, compared with rabbit sera raised against b. ruminicola subsp. ruminicola, b. ruminicola subsp. brevis and selenomonas ruminantium. the titers of sera from colostrum-deprived calves were negligible level, while those of sera fro ... | 1990 | 2313950 |
| cellulose digestion and cellulase regulation and distribution in fibrobacter succinogenes subsp. succinogenes s85. | fibrobacter succinogenes subsp. succinogenes s85 initiated growth on microcrystalline cellulose without a lag whether inoculated from a glucose, cellobiose, or cellulose culture. during growth on cellulose, there was no accumulation of soluble carbohydrate. when the growth medium contained either glucose or cellobiose in combination with microcrystalline cellulose, there was a lag in cellulose digestion until all of the soluble sugar had been utilized, suggesting an end product feedback mechanis ... | 1990 | 2339881 |
| regulation and distribution of fibrobacter succinogenes subsp. succinogenes s85 endoglucanases. | the distribution of endoglucanase activities in cultures of fibrobacter succinogenes subsp. succinogenes s85 grown on different carbon sources was examined by a variety of biochemical and immunological techniques. total culture endoglucanase activity was primarily cell associated and was expressed constitutively, although synthesis of endoglucanase 1 (eg1) was repressed by cellobiose. western immunoblotting showed that eg1 and eg3 were released into the culture fluid during growth, while eg2 rem ... | 1990 | 2339882 |
| white noise analysis of temporal properties in simple receptive fields of cat cortex. | we studied the linear and nonlinear temporal response properties of simple cells in cat visual cortex by presenting at single positions in the receptive field an optimally oriented bar stimulus whose luminance was modulated in a random, binary fashion. by crosscorrelating a cell's response with the input it was possible to obtain the zeroth-, first-, and second-order wiener kernels at each rf location. simple cells showed pronounced nonlinear temporal properties as revealed by the presence of pr ... | 1990 | 2390534 |
| distribution of immunoglobulin-containing cells in calves inoculated orally with ruminal bacteroides succinogenes and selenomonas ruminantium. | distribution of immunoglobulin(ig)-containing cells was investigated in calves inoculated orally with live organisms of both bacteroides succinogenes and selenomonas ruminantium. pathological changes and many ig-containing cells were observed in calves which inoculated three times at 2, 3 and 26 days of age. follicular germinal center was increased in number and size of the lymph nodes associated with the forestomach, suggesting activation of lymph apparatus. in the associated lymph nodes, igg-c ... | 1990 | 2391773 |