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a 16-fold semi-microelectrode for intracortical recording of field potentials.a 16-fold semi-microelectrode with 10 x 10 sq. microns contacts at distances of 150 microns, produced by means of thin-film technology, is described. these small dimensions are required to study the laminar electrophysiological differences within the cerebral cortex.197991493
presence of the asialoglycoproteins-binding protein in primary monolayer culture of adult rat hepatocytes [proceedings]. 197993914
comparison of antigen-specific i-region-associated cell interaction factors.two basic types of factors reacting with anti-i region (anti-ia) antisera are compared, those derived from macrophage-like antigen presenting cells and others derived from t-lymphocytes, of either the suppressor or helper type. despite the common property of reacting with anti-ia antisera, the two sets of factors differ by many criteria. macrophages, upon culture with antigen, release complexes of ia antigen and a fragment of the original immunogen. this material is only produced by responder ma ...197993870
revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov.hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ...19911704793
the indirect rat mast cell degranulation test reconsidered.the indirect rat mast cell degranulation test for the detection of human reaginic antibodies was reinvestigated. when the mast cells were incubated with human reaginic serum at concentrations exceeding approximately 3%, a massive, non-specific histamine release occurred, irrespective of the presence or absence of allergen. at tolerated serum concentrations (below 3%), the allergen concentration did not influence the release process. we conclude that the indirect rat mast cell test is not applica ...197990655
comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis.to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ...19911800380
the use of avidin-biotin interaction in immunoenzymatic techniques.biotin was covalently attached to antibodies, antigens and enzymes, and the effects of this labeling on the antigen and antibody binding capacity and on enzymatic activity were tested. based on avidin-biotin interaction, the labeled proteins were used in quantitative enzyme-immunoassay and enzyme-immunohistochemical staining procedures. two procedures were developed. in the first procedure, named the bridged avidin-biotin (brab) technique four steps were used sequentially in order to quantify or ...197990074
distribution of myosin isoenzymes among skeletal muscle fiber types.using an immunocytochemical approach, we have demonstrated a preferential distribution of myosin isoenzymes with respect to the pattern of fiber types in skeletal muscles of the rat. in an earlier study, we had shown that fluorescein-labeled antibody against "white" myosin from the chicken pectoralis stained all the white, intermediate and about half the red fibers of the rat diaphragm, a fast-twitch muscle (gauthier and lowey, 1977). we have now extended this study to include antibodies prepare ...197990047
studies on histamine release induced by compound 48/80 and peptide 401 [proceedings]. 197988890
benign prostatic hyperplasia in an xx man.a sixty-nine-year-old white phenotypic male who was being investigated for a myeloproliferative disorder, was found to have an xx karyotype in all cells examined in bone marrow, lymphocytes, and skin fibroblast cultures. despite essentially no testosterone in the plasma, he also suffered from severe prostatic hyperplasia, a finding not reported previously in patients with this genotype. while endocrine studies showed normal follicle-stimulating hormone, the luteinizing hormone level was twice th ...197988115
multivariate analyses of cellular fatty acids in bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp.the genera bacteroides, wolinella, and campylobacter contain several similar species that require taxonomic revision. fatty acid profiles of whole bacterial cells have proven useful for taxonomy. in this study, cellular fatty acids from bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. were identified and quantitated by gas chromatography and gas chromatography-mass spectrometry, and the data were subjected to principal component analyses. bacteroides fragilis, the type s ...19911993755
selective medium for bacteroides gracilis. 19911993761
pattern of milk flow in breast-fed infants.122 six-day-old infants were test-weighed during breast feeding. cross-sectional data collection avoided repeated interruptions of individual feeds. milk flow conformed to a definite pattern: a mean of 50% of the feed from each breast was taken in the first two minutes of sucking and a mean of 80-90% was taken by four minutes. the second half of a ten-minute feed from either breast was almost non-nutritive. preliminary studies suggest that the pattern is the same after one month of lactation.197987962
immunochemical studies of guinea pig progesterone-binding plasma protein.an antiserum specific for guinea pig progesterone-binding plasma protein (pbp) has been prepared. using a very sensitive immunoenzymatic assay, pbp could be detected not only in pregnant guinea pig plasma (970 microgram/ml of plasma at day 40--60 of pregnancy), but also in the plasma of fetuses (2.77 micrograms/ml), umbilical arteries (1.79 micrograms/ml), umbilical vein (2.90 micrograms/ml), and in amniotic fluid (0.47 microgram/ml). the protein was also found in low concentration in the plasma ...197885546
highly purified insulins. 197985008
vitamin e kinetics after intraperitoneal administration of dl-alpha-tocopherol and dl-alpha-tocopherol acetate in sheep.the disposition of radiotocopherol after intraperitoneal administration of 14c-labelled-dl-alpha-tocopherol (1 microci/kg bw, dose 1) and 3h-labelled-dl-alpha-tocopherol (4 microci/kg bw dose 4) and of d-alpha-tocopherol, after intraperitoneal administration of dl-alpha-tocopherol acetate (100 mg/kg bw) was investigated in sheep. plasma samples were taken at regular intervals after dosing and assayed for radioactivity and d-alpha-tocopherol. plasma profiles were modelized using a compartmental a ...19921610075
effects of bradykinin on the hemodynamics of tumor and granulating normal tissue microvasculature.bradykinin (bk) is an important endogenous mediator of microvascular flow modulation. since the structure of the microcirculation is very different in tumor tissues than in normal tissues, bradykinin may elicit different responses in tumors. this study was designed to test the hypothesis that local administration of bradykinin increases blood flow preferentially in normal tissue relative to adjacent tumor tissue, resulting in a "vascular steal" phenomenon. microvessel diameters (d), velocities ( ...19921594761
the early colonization of gram-negative anaerobic bacteria in edentulous infants.the occurrence of oral gram-negative anaerobes was examined in 30 edentulous infants (mean age 3 months, range 1-7 months). one pooled swab sample from mucosal surfaces (cheeks, palate, tongue) and one saliva sample was taken from each infant. the samples were cultured aerobically and anaerobically using non-selective and selective media. prevotella (bacteroides) melaninogenica was the most frequently isolated anaerobe, found in 70% of the infants. the other common anaerobes were fusobacterium n ...19921528621
detubularization-induced contractile response change of the ileum following ileocystoplasty.we reported previously that following ileocystoplasty the structure and pharmacologic response of the implanted ileum changes towards that of the bladder. specifically, the relaxation response to alpha adrenergic (methoxamine) and purinergic (atp) stimulation reverses to a contractile response one month after the ileal segment is surgically made part of the urinary bladder. the present study was designed to investigate possible signals for this change and also to determine whether bladder respon ...19921351953
[human chorionic prealbumin: its identification, physicochemical properties and detection in the blood serum in trophoblastic disease].human chorionic prealbumin (hcpa) was immunochemically identified and isolated in pure form from immature placenta, particularly, from chorion. comparative immunodiffusion analysis shows that hcpa differs from the known placenta-specific proteins. hcpa is a glycoprotein with mw about 100 kd determined by gel-filtration and 45 kd by sds polyacrylamide gel electrophoresis. by immunodiffusion hcpa was not found in fetal and adult tissues and in biological fluids. hcpa was discovered in patients wit ...19882848598
cytochrome composition and oxygen-dependent respiration-driven proton translocation in wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis.the membrane fractions of the microaerobically grown type strains of wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis contained membrane-bound cytochrome b, cytochrome c, and co-binding cytochrome c. soluble cytochrome c and co-binding cytochrome c were also present. although b. gracilis is oxidase negative, it possessed cytochrome c. with h2 or formate as the electron donor, proton efflux from anaerobic cells occurred upon addition of a pulse of oxygen. with f ...19921325865
microbiology of healthy and diseased periodontal sites in poorly controlled insulin dependent diabetics.a group of poorly-controlled insulin dependent diabetes mellitus (iddm) patients were examined in a cross-sectional design for total microbial levels, microbial incidence, and the percent levels of selected periodontal microorganisms. these organisms were selected on the basis of prior reports that associated them with either periodontal disease or health. one periodontally-healthy and one periodontally-diseased site were examined in each iddm patient. increased levels of the periodontal pathoge ...19921315389
in vitro activity of meropenem and other agents against oral bacteria.the susceptibility of 106 oral bacteria to meropenem, a new carbapenem antimicrobial, penicillin v, tetracycline and metronidazole was assessed by an agar dilution method. meropenem was the most effective of the antimicrobials, with all strains tested being inhibited at < or = 1 mg/l. a number of strains were resistant to penicillin v, particularly bacteroides gracilis, and tetracycline resistance was widespread amongst the streptococci tested. metronidazole was extremely effective against oblig ...19921286575
[effect of x-rays on human adult skin explants]. 19751233920
effect of repeated bloodletting on the incidence of 2,7-faa-induced rat leukemia.the oral administration of n,n'-2,7-fuorenylenebisacetamide (2,7-faa) induced leukemia, especially mature granulocytic leukemia, in rats. 2,7-faa was administered to the rats of the wistar strain and a small amount of bloodletting was repeated for a long period. the incidence of leukemia and mature granulocytic leukemia became elevated by bloodletting. the induction of mature granulocytic leukemia might be increased by the promotion of granulopoiesis which had been suppressed by 2,7-faa.19761068628
effectiveness of orally administered bordetella pertussis vaccine in mice.oral administration of killed bordetella pertussis organisms to mice results in increased resistance to an intracerebral infection with virulent b. pertussis cells. the rate of survival is dependent on the dose of antigen. but besides specific systemic immunity, which is persistent over a long period, also transient non-specific resistance is increased. these effects are evidently induced without penetration of bacterial substances into the circulation.1976182602
the effect of mechanical stretching of the myosin rod component (fragment lmmmm s-2) on the atpase activity of myosin.the binding of myosin to nylon fiber gives immobilized myosin with a considerable atpase activity. treatment of immobilized enzyme with papain results in the entire atpase activity (known to be concentrated in myosin heads, (fragment hmm s-1)) being replaced from the fiber into the solution; this means that myosin is chemically bound to the fiber via its rod part (fragment lmm+hmm s-2). when nylon fiber is mechanically stretched, the atpase activity of myosin attached to it sharply decreases; af ...1978149560
biologic activity of soluble collagen-anticollagen immune complexes as demonstrated by passive cutaneous anaphylaxis.collagen-anticollagen immune complexes were prepared by gel filtration. complex formation was chemically and serologically demonstrated. complexes eluting in gammam- and gammag-peaks of antigen-antiserum mixture showed strong positive reactions in pca experiments performed with 20 guinea pigs. control preparations of gammam- and gammag-peaks of antiserum devoid of antigen gave significantly lower pca-response. the positive reaction of control preparations can be explained in part by a certain de ...1975127469
the possible role of tightly bound adenine nucleotides in oxidative and photosynthetic phosphorylation.the tightly bound nucleotides of the beff-heart mitochondrial atpase are released during cold inactivation followed by ammonium sulfate precipitation. during incubation at 0 degrees c the sedimentation coefficient (s20w) of the atpase first declines from 12.1s to 9s. prolonged incubation or precipitation with ammonium sulfate leads to dissociation of the 9s component into subunits with s20w of 3.5s. the 9s component still bears bound nucleotides which exchange more extensively and rapidly with a ...1975127089
comparative study of aggressive behaviour after injection of cholinomimetics, anticholinesterases, nicotinic, and muscarinic ganglionic stimulants into the cerebral ventricles of conscious cats: failure of nicotinic drugs to evoke aggression. 1979106424
passage of immunoglobulins from plasma to the oral cavity in rhesus monkeys.the passage of immunoglobulin from plasma to the oral cavity was studied in rhesus monkeys. immunoglobulins g, a and m were purified from pooled rhesus monkey serum, radiolabelled with 125i and injected intravenously into twelve monkeys. sequential samples of oral fluids were taken over a 24 h period and were assayed for radioactivity. radioactivity could be detected in crevicular fluid washings after 0.5 h in monkeys injected with igg and iga, and after 2 h in monkeys given igm. maximal levels ...1978104924
analysis of anti-sperm sera for t/t locus-specific antibody.an objective antiglobulin radioimmunoassay for the analysis of anti-spermatozoa antisera has been used to characterize more than 30 alloantisera. each of these antisera was raised against spermatozoa from mice carrying t/t regin mutations. whilst all of the antisera had considerable activity for mouse spermatozoa, none of them was found to contain antibodies specific for t/t region gene products. a detailed analysis of a single serum revealed that its activity is for antigens which are non-polym ...197995255
atypical structure of the 23s ribosomal rna molecule in certain oral bacteria.ribosomal rna (rrna) isolated from wolinella recta and seven related bacteria was examined by agarose gel electrophoresis. the 23s rrna molecule could not be detected in w. recta, wolinella curva, bacteroides gracilis, or bacteroides ureolyticus. in place of the 23s molecule, there were three smaller molecules of approximately 1700, 650, and 600 bases designated 23s alpha, 23s beta, and 23s delta, respectively. an intact 23s rrna molecule could be isolated from wolinella succinogenes, campylobac ...19911710629
wolinella recta, wolinella curva, bacteroides ureolyticus, and bacteroides gracilis are microaerophiles, not anaerobes.although the nonfermentative, asaccharolytic, putative anaerobes wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis are phylogenetically related to the true campylobacters, the type strains of these species exhibited o2-dependent microaerophilic growth in brucella broth and on brucella agar. the optimum o2 levels for growth of these strains ranged from 4 to 14% in brucella broth and from 2 to 8% on brucella agar, when h2 was provided as the electron donor. no gro ...19911854636
identification, and susceptibility to seven antimicrobial agents, of 61 gram-negative anaerobic rods from periodontal pockets.sixty-one cultures of gram-negative anaerobic rods were isolated from deep periodontal pockets of patients with rapidly progressive periodontitis. isolates were speciated as bacteroides gingivalis (18 isolates), bacteroides intermedius (8), bacteroides oris (1), bacteroides gracilis (17) and fusobacterium nucleatum (17). their susceptibilities, to seven antimicrobial agents, were determined in vitro using a plate dilution technique. amoxycillin and amoxycillin with clavulanic acid were active ag ...19911901873
bacteroides ureolyticus (ntu) medium for the selective recovery of bacteroides gracilis.bacteroides gracilis is a gram-negative anaerobic bacillus which requires formate and fumarate for growth; it has been implicated in periodontal disease and serious infections of the head and neck. in this study, bacteroides ureolyticus (ntu) medium was tested for its ability to allow the growth of b. gracilis and other formate-fumarate requiring gram-negative anaerobes and to enable the recovery of these organisms from clinical specimens. all reference strains grew on ntu medium with the except ...19911942000
cardiac-specific troponin-i radioimmunoassay in the diagnosis of acute myocardial infarction.the cardiac isotype of the myofibrillar contractile protein, troponin-i, is located specifically in the mammalian heart. a sensitive radioimmunoassay has been developed to detect human and nonhuman primate cardiac troponin-i in serum down to 10 ng/ml. immunochemical cross reactivity with skeletal troponin-i was only 2% and was species nonspecific. normal patient levels of cardiac troponin-i are about 10 ng/ml. in patients with acute myocardial infarction (n = 32), serum cardiac troponin-i was el ...19873591601
severe illness associated with group a-hemolytic streptococcal infections.the wisconsin division of health (doh) began surveillance for severe illnesses associated with group a beta-hemolytic streptococcus (gabs) infections in late 1989 to describe the current epidemiologic features and clinical spectrum of these infections in the state. severe illness was defined by the isolation of gabs from the blood or by the development of one or more of the following in a patient infected with gabs: shock, extensive tissue injury, desquamating rash, disseminated intravascular co ...19911949873
fetal cortical transplants reduce the thalamic atrophy induced by frontal cortical lesions in newborn rats.fetal neocortical tissue was grafted into frontal cortex lesion cavities made in newborn rats. after survival periods extending up to 14 months, volumetric measurements of the total thalamus and of the lateral, medial and anterior thalamic compartments showed an amelioration of the thalamic atrophy that normally is found after cortical lesions. these results correspond to previous findings demonstrating interconnections between fetal cortical transplants and the host thalamus.19892710397
in vivo oxidants and pulmonary inflammation.endotoxemia is used as a model of the adult respiratory distress syndrome in chronically instrumented awake sheep. the toxic effects of endotoxin may be mediated through inflammatory cells and the release of toxic oxidants. n-acetylcysteine (nac) inhibits the in vivo effects of endotoxemia in chronically instrumented unanesthetized sheep. nac also functions as a free radical scavenger in vitro. it is possible that the protective effects of pretreatment with nac result from nac's ability in vivo ...19863697543
[lithotripsy by ultrashort pulsation. experimental study in renal lithiasis in the dog].a lithotriptor delivering ultrashort shockwaves and designed for use under ultrasonographic guidance has recently been developed. immersion in water is unnecessary. the device was experimented in renal lithiasis in dogs. twenty-two animals were treated. in seven, who served as controls, the safety of shots delivered in particular to the lungs, liver, bones and kidneys was demonstrated. furthermore, no cardiac rhythm disorders were recorded. a human stone was implanted in 15 animals. the stone wa ...19863707073
an evaluation of the multiple-breath nitrogen washout as a pulmonary function test in dairy cattle.multiple-breath nitrogen washouts (mbnw) were performed with 21 adult dairy cows (seven normal cows tested on two days each, four clinically normal cows tested on six days, and ten cows with respiratory disease tested on one day each). the functional residual capacity (frc), lung clearance index (lci), becklake index (bi), mixing ratio (mr), pulmonary n2 clearance delay (pcd), index of distribution of inspiration (idi) and ventilatory efficiency (eff) were calculated for each mbnw. the lci, mr, ...19892713778
in vitro activities of 17 antimicrobial agents against the formate/fumarate-requiring, anaerobic gram-negative bacilli.the in vitro activities of 17 antimicrobial agents were evaluated against 46 clinical isolates of formate/fumarate-requiring anaerobic gram-negative bacilli. strains of bacteroides ureolyticus (23) were almost uniformly susceptible to the tested antimicrobials, whereas strains of bacteroides gracilis (18) showed some striking resistance with penicillin active against only 67%, the cephalosporins active against 67%-89%, and clindamycin active against 67%. although few strains of wolinella species ...19863757477
identification of an additional sendai virus non-structural protein encoded by the p/c mrna.peptide antiserum and monoclonal antibodies have detected a previously unrecognized small non-structural protein in sendai virus-infected cells. this protein, designated x, appears to represent the c-terminal 95 amino acids of the p protein reading frame. the x protein appears to be almost as abundant as the p protein on a molar basis in vivo. no evidence of a precursor-product relationship between the x and p proteins could be found.19872821189
[role of alpha-1 and alpha-2-adrenoreceptors in achieving the compensatory response of the hemostasis system to plasmin]. 19882849566
the challenge of malaria vaccine: trials and tribulations. 19872880172
the effect of metoclopramide treatment on diabetic cystoparesis. 19882897479
wolinella recta, campylobacter concisus, bacteroides gracilis, and eikenella corrodens from periodontal lesions. 19872957486
a computer-aided analysis of the effect of peripheral nerve transection on tmpase activity of substantia gelatinosa rolandi. 19863020818
studies on the induction of serum hemopexin by pentobarbital and polycyclic hydrocarbons. 19724119360
striatal tyrosine hydroxylases with high and low affinity for tyrosine: implications for the multiple-pool concept of catecholamines. 19734148015
oxygen consumption of the germinal epithelium in psoriatic human skin as measured by the cartesian diver micro-gasometer. 19684185292
regulation of rat liver beta-hydroxy-beta-methylglutaryl-coa reductase activity by cholesterol. 19734202331
regional localization of cd18, the beta-subunit of the cell surface adhesion molecule lfa-1, on human chromosome 21 by in situ hybridization.the gene coding for the beta-subunit of the cell surface adhesion glycoprotein lfa-1 has been localized to the tip of the long arm of chromosome 21 at 21q22.1-qter.19883073708
characterization of the ksga gene of escherichia coli determining kasugamycin sensitivity.in the plasmid puc8ksga7, the coding region of the ksga gene is preceded by the lac promoter (plac) and a small open reading frame (orf). this orf of 15 codons is composed of nucleotides derived from the lacz gene, a multiple cloning site and the ksga gene itself. the reading frame begins with the atg initiation codon of lacz and ends a few nucleotides beyond the atg start codon of ksga. the ksga gene is not preceded by a shine-dalgarno (sd) signal. cells transformed with puc8ksga7 produce activ ...19873122846
[effect of eicosanoids on the accumulation of cholesterol and the proliferation of subendothelial cells in the human aorta].the effect of prostacyclin and stable thromboxane analog a2 on endothelial culture of human aorta was studied. it was shown that prostacyclin inhibited accumulation of cholesterol in the cells and their proliferation, while thromboxane exhibited an opposite effect. calcium antagonists potentiated effects of prostacyclin and inhibited them in respect to thromboxane. screening of a number of synthetic agents affecting arachidonic acid metabolism was carried out. it was found that lipoxygenase inhi ...19883122858
an analysis of the effects of taurine on cellular and extracellular calcium mobilization in rabbit ear artery. 19873124515
synthetic aci-reductones: 3,4-dihydroxy-2h-1-benzopyran-2-ones and their cis- and trans-4a,5,6,7,8,8a-hexahydro diastereomers. antiaggregatory, antilipidemic, and redox properties compared to those of the 4-substituted 2-hydroxytetronic acids.synthetic procedures for the elaboration of aci-reductones belonging to the 6- or 7-mono- or bis-substituted-3,4-dihydroxy-2h-1-benzopyran-2-ones (6-10) and their cis- and trans-4a,5,6,7,8,8a-hexahydro diastereomers (11, 12) are described. hexahydrobenzopyranone aci-reductones were conveniently prepared by using meldrum's synthon (2,2-dimethyl-1,3-dioxane-4,6-dione, 49). certain of these substances were evaluated for antilipidemic activity in the cholesterol-fed rat model, and all analogues were ...19883133477
suppressor cells in the rabbit appendix. 19744547314
micro in vitro test for chloroquine resistance of plasmodium falciparum: discrepancies attributable to batch variation of plates. 19873318026
pulsatile secretion of growth hormone and insulin in relation to feeding in rats.in unrestrained male wistar rats chronically implanted with intracardiac catheters, blood samples were taken every 20 min throughout the 24 h of the diurnal cycle. plasma concentrations of growth hormone (gh), insulin, and glucose were measured. the pattern of food intake was continuously monitored. the existence of 3-h pulsatile cycles of gh secretion was confirmed. in addition, short bursts of insulin secretion were observed in the middle of every second gh peak-to-peak interval. food intake a ...19873318507
effects of hormone deprivation on the karyometric development of the medial and lateral preoptic area of the male mouse. ii. hypothyroidism.we have analysed the karyometric development of the medial and lateral preoptic area of the hypothalamus in two groups of experimentally hypothyroid male mice, from the 25th to the 160th postnatal day. in one group, animals were treated with propylthiouracil from birth until the day of sacrifice. in the second experimental group animals were treated only until the 35th postnatal day, and thereafter sacrificed at the 45th, 55th, 85th and 160th postnatal day. we compare the results of both groups ...19873440835
cell division promoting activity of naturally occurring dehydrodiconiferyl glucosides: do cell wall components control cell division?naturally occurring isomers of the dehydrodiconiferyl glucosides have been isolated from vinca rosea crown gall tumors and have been tested for cell division promoting activities in the tobacco pith and leaf assay systems. the enantiomeric isomers a and b are active, although they are required at concentrations up to 2 orders of magnitude higher than zeatin riboside to promote comparable growth. we estimate that the active dehydrodiconiferyl glucosides are present in rapidly growing tissues (tum ...19873469655
effect of treatment with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine/mptp/on mouse substantia nigra in vitro. fluorescence and electron microscopic examination. 19873501590
inactivation of anaerobic bacteria by various photosensitized porphyrins or by hemin.the photodynamic effects of deuteroporphyrin (dp), hematoporphyrin derivative (hpd), hematoporphyrin (hp), or protoporphyrin (pp) on a variety of anaerobic microorganisms were examined in this study. the majority of the species, among the 350 strains tested, were inhibited by concentrations of < or = 2.5 micrograms/ml of light-activated dp. species found to be resistant to this treatment included bilophila wadsworthia, fusobacterium mortiferum, fusobacterium varium, and bacteroides gracilis. the ...19947765091
characterization of wolinella spp., campylobacter concisus, bacteroides gracilis, and eikenella corrodens by polyacrylamide gel electrophoresis.the small asaccharolytic, nonpigmenting gram-negative rods of the human oral cavity are difficult to differentiate from each other. protein profiles of sonicated cells of wolinella species, campylobacter concisus, bacteroides gracilis, and eikenella corrodens were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized with a silver stain. the gels were scanned with a laser densitometer, and the similarity of strains was computed by determining correlation coefficien ...19863533976
selective medium for isolation of bacteroides gracilis.a new medium selective for bacteroides gracilis was developed. the medium is tryptic soy agar (difco laboratories, detroit, mich.) containing nalidixic acid, teicoplanin, sodium formate, sodium fumarate, and potassium nitrate. all 18 strains of b. gracilis tested grew with only minimal inhibition. most of the other 214 organisms tested, including most bacteroides species, other anaerobes, and a substantial number of facultative anaerobes, were significantly inhibited by the medium. in a diagnost ...19902144297
in-vitro susceptibilities of bacteroides gracilis, fusobacterium mortiferum and f. varium to 17 antimicrobial agents. 19902211439
effects of the methyl xanthine s 9795 on isolated bronchi of the dog.the xanthine derivative 1-methyl 3-isobutyl 8-(2-ethyl[1-(4-diphenylmethyl piperazinyl)])3, 7-dihydro (1h) purine 2, 6-dione (s 9795) is a potent inhibitor of bronchoconstriction in vivo. the aim of the present study was to analyze the effects of s 9795 in vitro and determine whether s 9795 affects the autonomic nerves, the epithelium or the smooth muscle of the bronchial wall. s 9795 had an inhibitory effect on the contractile responses evoked by acetylcholine and by electrical stimulation of t ...19902395105
in vitro activity of bay y3118 against anaerobic bacteria.the antimicrobial activity of a new quinolone, bay y3118, was determined against 326 strains of anaerobic bacteria and compared with the activities of ampicillin-sulbactam, cefotetan, clindamycin, imipenem, metronidazole, and sparfloxacin. the national committee for clinical laboratory standards-approved wadsworth agar dilution technique with brucella-laked blood agar was used throughout the study. breakpoints used to determine the percent susceptible were 2 micrograms/ml for bay y3118 and sparf ...19938285647
characterization of an unusual microaerophilic organism closely related to bacteroides gracilis. 19938324116
empyema due to bacteroides gracilis: case report and in vitro susceptibilities to eight antimicrobial agents.we describe a patient with empyema due to bacteroides gracilis and a viridans streptococcus. therapy with both cefoxitin and imipenem failed, and the patient remained symptomatic even after thoracotomy was performed. clinical response occurred after initiation of clindamycin therapy. the susceptibilities of the patient's isolate, other clinical strains, and the reference strain were determined by an agar dilution method. the isolates were generally susceptible to all the agents tested; however, ...19938324129
fetal cortical transplants reduce the thalamic atrophy induced by frontal cortical lesions in newborn rats.fetal neocortical tissue was grafted into frontal cortex lesion cavities made in newborn rats. after survival periods extending up to 14 months, volumetric measurements of the total thalamus and of the lateral, medial and anterior thalamic compartments showed an amelioration of the thalamic atrophy that normally is found after cortical lesions. these results correspond to previous findings demonstrating interconnections between fetal cortical transplants and the host thalamus.19892710397
helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis.four strains of a novel helicobacter species were isolated from the stomachs of cheetahs (acinonyx jubilatus) with gastritis. these isolates were phenotypically similar to helicobacter pylori. the isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37 degrees c, but not at 25 or 42 degrees c, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. the isolates did not ferment glucose, mannitol, inositol, sorbitol, r ...19938379970
in vitro activity of fk037, a new parenteral cephalosporin, against anaerobic bacteria.the activity of fk037, a new parenteral cephalosporin, was compared with those of cefpirome, ceftazidime, and flomoxef against 322 recent clinical isolates of anaerobic bacteria. a fastidious facultative anaerobe, gardnerella vaginalis, was also studied. fk037 inhibited 90% of isolates of peptostreptococcus anaerobius, peptostreptococcus asaccharolyticus, clostridium perfringens, mobiluncus spp., g. vaginalis, and porphyromonas gingivalis at < or = 0.78 micrograms/ml. the mics of fk037 for 50 an ...19938517721
nitrogen conversion factors for the proteinaceous content of gums permitted as food additives.nitrogen conversion factors for gum arabic (acacia senegal (l.) willd.), gum tragacanth (asiatic astragalus spp.), gum karaya (sterculia spp.), guar gum (cyamopsis spp.), locust bean (carob) gum (ceratonia spp.), tara gum (caesalpinia spp.), and xanthan gum (xanthomonas campestris) have been calculated from data for the amino acid compositions of their proteinaceous components. the factors derived differ from the arbitrary values (5.7 or 6.25) at present specified by international regulatory aut ...19863743832
epidemiologic studies on possible health effects of intake of pyrolyzates of foods, with reference to mortality among japanese seventh-day adventists.to elucidate the effect of intake of mutagenic and/or carcinogenic pyrolysis products of proteins and amino acids on carcinogenesis in man, we have undertaken two epidemiologic cohort studies: one concerning the possible association of broiled fish consumption with cancer and the other concerning the cancer mortality among japanese seventh-day adventists. the main findings of these studies are described.19863757950
in vitro activities of trovafloxacin against 557 strains of anaerobic bacteria.the antimicrobial activity of trovafloxacin for 557 strains of anaerobic bacteria was determined by the national committee for clinical laboratory standards-approved wadsworth agar dilution technique. the species tested included bacteroides fragilis (n = 91), other members of the b. fragilis group (n = 130), campylobacter gracilis (n = 15), other bacteroides spp. (n = 16), prevotella spp. (n = 49), porphyromonas spp. (n = 15), fusobacterium spp. (n = 62), bilophila wadsworthia (n = 24), sutterel ...19968878617
the sfia11 mutation prevents filamentation in a response to cell wall damage only in a reca+ genetic background.n-alpha-palmitoyl-l-lysyl-l-lysine dihydrochloride ethyl ester (pll) at sublethal doses causes filamentous growth of e. coli strains except sfia mutants, which divide normally in its presence. pll does not elicit the sos responses as judged by lambda prophage induction, an increase of reca protein synthesis or induction of the sfia operon in a sfia::lacz fusion strain. thus, it appears that filamentation caused by pll is not an sos function and might be the result of membrane damage by pll, whic ...19853911030
recently described clinically important anaerobic bacteria: taxonomic aspects and update.a new method of identifying bacteria, phylogenetic 16s rrna sequencing, has led to major reorganizations among most genera of anaerobic bacteria. the pigmented prevotella species now comprise seven species including p. nigrescens and p. tannerae; p. intermedia/p. nigrescens-like organisms await inclusion. the former mitsuokella dentalis and hallella seregens were transferred to prevotella as one species, p. dentalis. p. enoeca is a new nonpigmenting prevotella. the genus porphyromonas currently ...19979310640
recently described clinically important anaerobic bacteria: medical aspects.there is still inadequate information on the role of certain newly described or reclassified anaerobes in disease processes, on their normal sites of carriage, and on their antimicrobial susceptibilities. herein, we summarize this information (most of the literature reviewed is from the past 5 years, but a few of the articles are approximately 10 years old). porphyromonas species had seemed to be relatively nonpathogenic, but recent work indicates that this belief is incorrect. p. gingivalis, p ...19979310641
dental implant infections.dental implants provide a restorative tool to support crowns, bridge abutments, and removable dentures. osseointegrated implants are titanium posts that are surgically implanted in alveolar bone. a tight immobile bond (osseointegration) forms between bone and titanium, and prosthetic and restorative fixtures are attached to the implants. titanium implants differ from natural teeth, which may make them more susceptible to mechanical stress. a small proportion of implants are not successful and ma ...19979310682
canine brucellosis in a household.three cases of canine brucellosis from the same household were discovered during a serosurvey of privately owned dogs. one dog was found to be seropositive and culture-positive, 1 dog was seropositive, and 1 dog had suspect test results, with eye lesions suggestive of brucella canis infection. all dogs had signs seen with b canis infection, but none was pathognomonic. although pathologic findings in 2 of the dogs were consistent with findings reported by other investigators, none was conclusive ...19853921503
[significance of synthetic calcium phosphate ceramics as a bone replacement material].with reference to the chemical and crystallographic differences, the line is drawn against bone substitute material produced from biological material. extensive animal experiments demonstrated the good healing properties when using calcium phosphate ceramics in bony defects. in the long term, there was more new bone formed after filling up the defect with calcium phosphate ceramics than when using autologous bone grafts. clinically, there is an indication for using this material to fill up bone ...19863960537
[significance of synthetic calcium phosphate ceramics as a bone replacement material].with reference to the chemical and crystallographic differences, the line is drawn against bone substitute material produced from biological material. extensive animal experiments demonstrated the good healing properties when using calcium phosphate ceramics in bony defects. in the long term, there was more new bone formed after filling up the defect with calcium phosphate ceramics than when using autologous bone grafts. clinically, there is an indication for using this material to fill up bone ...19863960537
microbial complexes in subgingival plaque.it has been recognized for some time that bacterial species exist in complexes in subgingival plaque. the purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 years) with (n = 160) or without (n = 25) periodontitis. the presence and levels of 40 subgingival taxa ...19989495612
bacteroides gracilis, an important anaerobic bacterial pathogen.clinical isolates of agar-pitting, formate-fumarate-requiring, anaerobic gram-negative bacilli were recharacterized in consideration of the species descriptions of bacteroides ureolyticus and the newly described b. gracilis, campylobacter concisus, and wolinella sp. during an 11-year period, 7.5% (101 of 1,341) of all specimens yielding anaerobes were found to contain an organism in this group. of the 71 isolates that were available for study, 43 were b. ureolyticus, 23 were b. gracilis, and 5 w ...19854056006
activities of gemifloxacin (sb 265805, lb20304) compared to those of other oral antimicrobial agents against unusual anaerobes.the activities of gemifloxacin (sb 265805, lb20304) and comparator agents were determined by an agar dilution method against 419 clinical strains of less-commonly identified species of anaerobes. gemifloxacin was generally more active than trovafloxacin against gram-positive strains by one to two dilutions. peptostreptococci (peptostreptococcus asaccharolyticus, peptostreptococcus magnus, peptostreptococcus micros, and peptostreptococcus prevotii) and porphyromonas spp. (porphyromonas asaccharol ...199910543754
activities of telithromycin (hmr 3647, ru 66647) compared to those of erythromycin, azithromycin, clarithromycin, roxithromycin, and other antimicrobial agents against unusual anaerobes.the comparative activity of telithromycin (hmr 3647) against 419 human anaerobic isolates was determined by the agar dilution method. at concentrations of </=0.5 microgram/ml, telithromycin was active against actinomyces israelii, actinomyces odontolyticus, bacteroides tectum, bacteroides ureolyticus, bacteroides gracilis (now campylobacter gracilis), porphyromonas spp. (including porphyromonas gingivalis and porphyromonas macacae), prevotella intermedia, prevotella heparinolytica, and almost al ...199910543769
profile of subgingival microbiota in children with primary dentition.eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). the samples were cultured for bacterial isolat ...200010791707
microbiological parameters associated with il-1 gene polymorphisms in periodontitis patients.polymorphisms in the cluster of il-1 genes have been significantly associated with the severity of adult periodontitis. the purpose of this study was to compare microbiological parameters in il-1 genotype negative and positive adult subjects with a range of periodontitis severities.200011073323
comparative in vitro activities of abt-773 against 362 clinical isolates of anaerobic bacteria.the activity of abt-773, a novel ketolide antibiotic, against clinical isolates of anaerobic bacteria was determined and compared to the activities of other antimicrobial agents. mics at which 90% of isolates were inhibited (mic(90)s) were </=0.06 microg/ml for actinomyces spp., clostridium perfringens, peptostreptococcus spp., propionibacterium spp., and porphyromonas spp. the mic(50)s and mic(90)s were </=0.06 and >32 microg/ml, respectively, for eubacterium spp., lactobacillus spp., clostridi ...200111120995
profile of subgingival microbiota in children with mixed dentition.a diversity of microbial species has been detected in children's oral flora at an early age. to investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. the samples were cultured for bacterial isolation anaerobica ...200011155173
some pharmacological properties of a synthetic oxytocin analogue [1-n-carbamoyl-hemicystine-2-o-methyltyrosine]-oxytocin (carbamoyl-methyloxytocin), an antagonist to the neurohypophysial hormones.1. a synthetic oxytocin analogue, [1-n-carbamoyl-hemicystine-2-o-methyltyrosine]-oxytocin (carbamoyl-methyloxytocin), has been tested as an antagonist to the actions of oxytocin and vasopressin on the uterus, the mammary gland and blood pressure.2. the analogue inhibited the response of the isolated rat uterus to both oxytocin and vasopressin without itself stimulating the uterus to contract. the responses to equipotent doses of oxytocin and vasopressin were inhibited equally. there was little o ...19704321760
some pharmacological properties of a synthetic oxytocin analogue [1-n-carbamoyl-hemicystine-2-o-methyltyrosine]-oxytocin (carbamoyl-methyloxytocin), an antagonist to the neurohypophysial hormones.1. a synthetic oxytocin analogue, [1-n-carbamoyl-hemicystine-2-o-methyltyrosine]-oxytocin (carbamoyl-methyloxytocin), has been tested as an antagonist to the actions of oxytocin and vasopressin on the uterus, the mammary gland and blood pressure.2. the analogue inhibited the response of the isolated rat uterus to both oxytocin and vasopressin without itself stimulating the uterus to contract. the responses to equipotent doses of oxytocin and vasopressin were inhibited equally. there was little o ...19704321760
inhibition of hepatic ribonuclease activity by chronic administration of phenobarbitone. 19704394767
comparative in vitro activity of ertapenem and 11 other antimicrobial agents against aerobic and anaerobic pathogens isolated from skin and soft tissue animal and human bite wound infections.we studied the comparative in vitro activity of ertapenem, a new carbapenem, against 240 aerobic and 180 anaerobic recent clinical bite isolates using an agar dilution method and an inoculum of 10(4) cfu/spot for aerobes and 10(5) cfu/spot for anaerobes. ertapenem inhibited 410/420 (98%) of the isolates tested at < or = 4 mg/l with only 4/5 campylobacter gracilis and 1/3 campylobacter rectus strains requiring . or = 16 mg/l for inhibition. ertapenem was only moderately active (mic 8 mg/l) agains ...200111679553
antigen-binding cells following contact-sensitization detected by rosette-formation. characterization of the receptors by inhibition of rosette-forming cells with anti-rabbit allotype and anti-class specific antisera. 19734541103
campylobacter gracilis and campylobacter rectus in primary endodontic infections.a species-specific nested polymerase chain reaction (pcr) assay was used to investigate the occurrence of campylobacter gracilis and c. rectus in primary root canal infections.200312657143
[evaluation of anaerobic flora in lung neoplasms].purpose of this study was to analyse the kind and frequency of anaerobic bacteria in the lower respiratory tract as well as susceptibility of the bacteria to antibiotics. material from 35 patients with lung tumour derived from tumour and post-tumoural area sample. collected sample was cultivated in anaerobic and aerobic conditions. anaerobic bacteria were found in 23 (66%) samples. totally there were 44 isolated of anaerobic bacteria strains. the most common g-negative strains belonged to genus ...200312959024
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