| bioisomerization of lindane in rats. | the major environmental problem associated with the use of gamma-hexachlorocyclohexane (gamma-hch, lindane) has been the appearance of the more oncogenic alpha- and beta- isomers as terminal residues in nature. to account for these residues it was suggested that gamma-hexachlorocyclohexane had been bioisomerized to the more stable alpha- and beta- isomers. in this study the effect of dose and duration of treatment on the proposed bioisomerization of gamma-hexachlorocyclohexane in the rat was inv ... | 1979 | 84852 |
| highly purified insulins. | | 1979 | 85008 |
| x-linked immunocompetence and epstein-barr virus oncogenesis. | | 1979 | 86133 |
| [intratypical antigenic differentiation of poliovirus strains circulating in mali by means of cross-adsorbed sera]. | the intratypic antigenic differentiation of poliovirus strains isolated from sick or healthy children in mali in 1975-1976 was performed. the method is based on the use of cross-adsorbed sera in the neutralization tests in which the virus is titrated by the plaque procedure or by the cytopathic effect. all the examined strains of serologic types i, ii, and iii were distinguished by the antigenic structure from the vaccine strains and were classified as "wild". quantitative differences in the ant ... | 1978 | 86237 |
| distribution of myosin isoenzymes among skeletal muscle fiber types. | using an immunocytochemical approach, we have demonstrated a preferential distribution of myosin isoenzymes with respect to the pattern of fiber types in skeletal muscles of the rat. in an earlier study, we had shown that fluorescein-labeled antibody against "white" myosin from the chicken pectoralis stained all the white, intermediate and about half the red fibers of the rat diaphragm, a fast-twitch muscle (gauthier and lowey, 1977). we have now extended this study to include antibodies prepare ... | 1979 | 90047 |
| the use of avidin-biotin interaction in immunoenzymatic techniques. | biotin was covalently attached to antibodies, antigens and enzymes, and the effects of this labeling on the antigen and antibody binding capacity and on enzymatic activity were tested. based on avidin-biotin interaction, the labeled proteins were used in quantitative enzyme-immunoassay and enzyme-immunohistochemical staining procedures. two procedures were developed. in the first procedure, named the bridged avidin-biotin (brab) technique four steps were used sequentially in order to quantify or ... | 1979 | 90074 |
| polyribonucleotide inhibition of ribonucleic acid directed deoxyribonucleic acid polymerase of mouse mammary tumor (type b) virus and simian sarcoma (type c) virus. | | 1979 | 90331 |
| the expression of a human b-lymphocyte antigen and surface membrane immunoglobulin by lymphoid cells from patients with lymphocytic lymphoma, multiple myeloma and benign monoclonal gammapathy. | | 1979 | 90449 |
| [animal experimental studies on the volume expanding effect of hydroxyethyl starch 40000 in the dog acute hemorrhagic shock]. | after acute hemorrhagic shock in dogs the hemodynamics of hydroxyethyl starch (mw: 40,000) are studied. the experiment shows that this plasma expander has a good efficiency for 3 to 4 hours. the indications of application of this colloidal solution are discussed. | 1979 | 90651 |
| antigens in penicillin allergy. iv. induction of ige antibody response in mice after daily treatment with contaminated but not with pure penicillin. | | 1979 | 90657 |
| a 16-fold semi-microelectrode for intracortical recording of field potentials. | a 16-fold semi-microelectrode with 10 x 10 sq. microns contacts at distances of 150 microns, produced by means of thin-film technology, is described. these small dimensions are required to study the laminar electrophysiological differences within the cerebral cortex. | 1979 | 91493 |
| trace polypeptides in cellular extracts and human body fluids detected by two-dimensional electrophoresis and a highly sensitive silver stain. | development of a highly sensitive silver stain permits the characterization of trace cellular and body fluid proteins separated by the two-dimensional electrophoresis technique of o'farrell. many of the proteins detected by the silver stain in urine, spinal fluid, amniotic fluid, and cells were undetected with the widely used coomassie blue stain. trace polypeptides observed in escherichia coli cell lysates with this silver stain could be detected previously only by growth in radioactive precurs ... | 1979 | 92027 |
| concert chromatography purification of mouse interferon [proceedings]. | | 1979 | 92244 |
| the effect of ablation of eosinophils on immediate-type hypersensitivity reactions. | the effect of ablation of eosinophils on hypersensitivity reactions in guinea-pigs was tested by administration of rabbit antiserum to the eosinophil (aes) and by administration of glucocorticoids. both aes and methylprednisolone ablated eosinophils from the blood and peritoneal cavity of test animals. neither administration of aes nor methylprednisolone, however, altered passive cutaneous or systemic anaphylactic reactions when compared to reactions occurring in control animals treated with nor ... | 1979 | 92457 |
| detailed monitoring of the effects of mannitol following experimental head injury. | the experimental model of a cerebral missile injury developed by crockard was used in three groups of rhesus monkeys treated with mannitol. one group received mannitol 15 minutes after being injured with a bb pellet at 90 m/sec impact. another group was wounded identically, but mannitol treatment was delayed until 1 hour after injury. the last group was wounded with the missile traveling at 180 m/sec, and mannitol was started 15 minutes after trauma. the data were contrasted with the results fro ... | 1979 | 106095 |
| betamethasone and the rhesus fetus: multisystemic effects. | in this controlled study of betamethasone administration to pregnant rhesus monkeys, using dosages per gram of fetal body weight similar to those reported in several human clinical studies, the most significant fetal pulmonary changes observed were increases in maximum lung volumes. the fact that comparable increases in peak volumes were demonstrated on saline filling supports our contention that these changes are related primarily to lung structural alterations rather than surfactant effects. a ... | 1979 | 106730 |
| [pseudomonas aeruginosa--the causative agent of infection in birds]. | pseudomonas aeruginosa was isolated from the viscera of diseased and dead birds (young and adult chickens) as well as from 9 poultry houses on seven farms. its typing was performed on the basis of the blue-green coloration of the cultures, their hemolytic activity, the pleasant odour of lime-tree, the growth in culturing at 42 degrees c, the presence of the pyocianin pigment, and the positive results of the cytochromoxidase test after gaby and hadley and the biochemical test after kovacs. the pa ... | 1975 | 127412 |
| [human genetics: a task for the public health service? (author's transl)]. | | 1977 | 143637 |
| a survey of intestinal parasitism in a community on the pan american highway route in eastern panama. | a survey of intestinal parasitism was conducted at yaviza, a town in eastern panama's province of darien. single stool specimens from 202 persons, representing 11 percent of the town's total population, were examined. in all, 181 (90 per cent) of the specimens examined were found positive for one or more potentially pathogenic parasites. the most common parasite found was trichuris trichiura, which was observed in 80 per cent of the samples. others were ascaris lumbricoides, in 62 per cent of th ... | 1975 | 167889 |
| intraperitoneal oxygen and carbon dioxide tensions in experimental adhesion disease and peritonitis. | intraperitoneal oxygen and carbon dioxide tensions were studied in rats during silica-induced adhesion formation or fecal peritonitis. measurements of po2 and pco2 in the abdominal cavity were performed by means of an implanted silastic tonometer. during active adhesion formation one to three weeks after administration of silica, the intra-abdominal po2 decreased by 50 per cent from normal whereas the intra-abdominal pco2 and the rate of oxygen consumption in the peritoneum were elevated. progre ... | 1975 | 170836 |
| steroid production by quartered and capsular rat adrenal gland in response to haemorrhage. | 60 min after rapid bleeding (1.5--2.0 per cent of b. w.) both aldosterone and corticosterone production rate by quartered rat adrenals were found to be elevated. however, no difference was observed in the rate of aldosterone and corticosterone production by capsular adrenals of sham operated and hypovolaemic rats. corticosterone production rate by decapsulated adrenals was much more higher after haemorrhage than in the control group. the same alterations could be observed incubating adrenal tiss ... | 1975 | 179800 |
| atp-ppi exchange activity of progesterone receptor. | progesterone receptor preparations from avian oviduct catalyze a pyrophosphate (ppi)-exchange reaction between atp and 32p-labeled ppi. the reaction requires atp exclusively and is mn++-dependent. this enzyme activity is detectable in receptor preparations that have been purified extensively by chromatography on atp-sepharose and deae-sephadex columns. polyacrylamide gel electrophoresis of purified preparations reveals a comigration of [3h]progesterone-receptor complex and the enzyme activity. t ... | 1976 | 185612 |
| [isolation of preparations of type b monoamine oxidase from liver mitochondria]. | | 1977 | 193582 |
| loss of regulation of lipid metabolism in morris hepatomas: a potential role for cytoplasmic binding proteins. | | 1977 | 205110 |
| apparent equilibrium constant between beta-adrenoceptors and a competitive antagonist on a cultured pacemaker cell of mammalian heart. | heart cells from newborn rats and kittens were cultured. in both species the rate of pacemaker activity of single, cultured cells was increased by low concentrations of catecholamines. tthe beta-adrenoceptor blocker (minus)-kl255 antagonized competitively the effects of both, (minus)-noradrenaline and (minus)-isoprenaline. a mean equilibrium constant of 3.6 times 10-10m between (minus)-kl255 and beta-adrenoceptors of single cells was estimated. | 1975 | 235102 |
| apparatus for uniaxial testing of soft human tissues in vitro. | | 1978 | 309051 |
| demonstration of thy-1 antigen on pluripotent hemopoietic stem cells in the rat. | three approaches were used to demonstrate the presence of thy-1 antigen on the surface of pluripotent hemopoietic stem cells in the rat. in the first, stem cells from fetal liver, neonatal spleen, and adult bone marrow were prevented from forming hemopoietic colonies in the spleens of irradiated recipients spleen (colony-forming unit assay) by incubation with antibodies to thy-1 antigen. highly specific rabbit heteroantiserum to purified rat brain thy-1 antigen and mouse alloantisera to thy-1.1- ... | 1978 | 309914 |
| a simple one-step hemolytic assay for c2 with c2-deficient human serum. | a simple one-step procedure has been developed for the molecular titration of c2 by utilizing the ability of the test material to restore the hemolytic activity of human serum selectively deficient in c2 (c2d serum). in this assay, equal volumes of ea (10(8) cells/ml), c2d serum (1/20), and a suitable dilution of a source of c2 were incubated at 37 degrees c for 60 min and the fraction of cells lysed was used to calculate the effective molecules of c2/ml test material. the assay can be used to t ... | 1977 | 321681 |
| superoxide dismutase in bacteroides fragilis and related bacteroides species. | superoxide dismutase (sod) activity was demonstrated in cell-free extracts of bacteroides fragilis, bacteroides vulgatus, bacteroides distasonis, bacteroides ovatus, and bacteroides thetaiotaomicron. the strains were grown under anaerobic conditions in trypticase soy broth, and the specific activity of sod in the extracts was, in most strains, higher than in cell-free extracts of escherichia coli b grown under anaerobic conditions. isoelectric focusing of the extracts in polyacrylamide gel demon ... | 1977 | 332713 |
| purification and immunohistochemical localization of rat brain myelin proteolipid protein. | | 1977 | 404395 |
| [problems of the disease due to mycobacteria other than tubercle bacilli: surgical treatment and indication (author's transl)]. | | 1979 | 522352 |
| protective efficacy of immunization with capsular antigen against experimental infection with bacteroides fragilis. | the protective efficacy afforded by immunization with the capsular antigen of bacteroides fragilis against abscess formation and bacteremia due to this organism was studied in an experimental rat model of intraabdominal sepsis. of unimmunized animals, animals immunized with methylated bovine serum albumin and complete freund's adjuvant, and animals immunized with lipopolysaccharide of bacteroides thetaiotaomicron, greater than 90% developed abscesses when challenged intraperitoneally with strain ... | 1979 | 528789 |
| synthesis of alpha-ketoglutarate by reductive carboxylation of succinate in veillonella, selenomonas, and bacteriodes species. | evidence for reductive carboxylation of succinate to synthesize alpha-ketoglutarate was sought in anaerobic heterotrophs from the rumen and from other anaerobic habitats. cultures were grown in media containing unlabeled energy substrates plus [14c]succinate, and synthesis of cellular glutamate with a much higher specific activity than that of cellular asparate was taken as evidence for alpha-ketoglutarate synthase activity. our results indicate alpha-ketoglutarate synthase functions in selenomo ... | 1979 | 533772 |
| factors affecting production of catalase by bacteroides. | several variables affected the production of catalase by members of the "bacteroides fragilis group" of anaerobic bacteria. both media yielded higher catalase levels than the respective agar media. addition of hemin to media after autoclave sterilization, rather than before, significantly increased production of catalase. both of these variables could be related to the available hemin concentration present in the medium being tested. significantly higher amounts of hemin were required for catal ... | 1978 | 730827 |
| production and some properties of catalase and superoxide dismutase from the anaerobe bacteroides distasonis. | the catalase level of bacteroides distasonis (atcc 8503, type strain) varied with the amount of hemin supplied to the medium when the cells were grown in either a prereduced medium containing 0.5% peptone, 0.5% yeast extract, and 1% glucose or in a prereduced, defined heme-deficient medium. the effect of hemin on catalase production could not be duplicated by ferrous sulfate or ferrous ammonium citrate. catalase activity reached peak values in late log phase, whereas superoxide dismutase specifi ... | 1977 | 845116 |
| laminarinase (beta-glucanase) activity in bacteroides from the human colon. | laminarin, a beta(1 leads to 3)-glucan similar to those found in plant cell walls, is fermented by some species of anaerobic bacteria from the human colon. laminarinase (ec 3.2.1.6) and beta-glucosidase (ec 3.2.1.21) activities were determined in strains representing bacteroides thetaiotaomicron, bacteroides distasonis, and an unnamed deoxyribonucleic acid homology group of bacteroides fragilis. in all three species, laminarinase activity was inducible by laminarin and was predominantly cell bou ... | 1977 | 879772 |
| new approach to caloric stimulation of the vestibular receptor. | a new caloric testing method is described. during continuous aural irrigation, fluid is switched between hot and cold values at times computed according to a mathematical model of heat conduction in the labyrinth area. as a result, the induced temperature difference across the lateral semicircular canal describes an approximately sinusoidal time course, reaching peak values of equal magnitude but opposite sign. the magnitude of the caloric stimulus may be selected by choosing appropriate irrigat ... | 1975 | 1081359 |
| [studies on the protein and amino acid metabolism of laying hens using 15n labeled casein. 15n concentration in the intestinal content and intestinal tissue in relation to the time of the last 15n administration]. | four laying hens with anus praeternaturalis were fed 15n casein for a period of 6 experimental days. each experimental bird thus received a 15n excess (15n') of 154.44 mg. 11.54 mg of 15n' were found in lysine 3.99 mg in histidine and 9.58 mg in arginine. 2 hens were slaughtered 48 hours after the last 15n dose; another 2 were sacrificed after 144 hrs. in this investigation the n content of the intestinal contents and in the proteins of the small and large intestine were determined. the average ... | 1975 | 1233975 |
| the distribution of serotonin-containing cells in the human alimentary tract. | the distribution of serotonin-containing cells (sc cells) in the human alimentary tract was studied by means of the fluorescent histochemical method of falck et al. on surgically resected specimens. no sc cells could be observed in the nonmucosal parts of the alimentary tract and in the squamous cell epithelium of the esophagus. the richest sc cells were observed in the upper portion of the small intestine (namely the duodenum and the jejunum). the rectum was relatively rich in the distribution ... | 1975 | 1234108 |
| german multicentre study on the in vitro susceptibility of bacteroides species. the german bacteroides study group. | in 1990 the first german multicentre study on the in vitro susceptibility of bacteroides species was completed. employing a commercially prepared microbroth dilution assay, nine participating institutions evaluated approximately 100 consecutive isolates of bacteroides species from relevant clinical specimens. a total of 911 strains (449 bacteroides fragilis, 201 bacteroides thetaiotaomicron, 79 bacteroides ovatus, 78 bacteroides vulgatus, 77 bacteroides distasonis, 25 bacteroides uniformis, 2 ot ... | 1992 | 1295762 |
| the isolation and characterisation of a major outer-membrane protein from bacteroides distasonis. | an outer-membrane protein (omp) was isolated from a clinical strain of bacteroides distasonis. changes in growth media did not appreciably affect the appearance of this protein in crude outer-membrane preparations examined by sds-page. however, the proportion of the protein relative to other omps was greater in 24-h cultures than in 48-h cultures. the protein could not be readily solubilised by various conventional detergent extraction techniques but treatment of the insoluble material at 100 de ... | 1992 | 1325560 |
| a new restriction endonuclease from bacteroides distasonis (bdii). | | 1990 | 1368524 |
| characterization of restriction endonuclease bdisi, an isoschizomer of sfei, from bacteroides distasonis strain s-7. | | 1991 | 1368669 |
| recovery of bacteroides fragilis group from clinical specimens following antimicrobial therapy. | over a period of 14 years (1973-1987), 3165 specimens submitted to the microbiology laboratory demonstrated the recovery of anaerobic bacteria. a total of 988 bacteroides fragilis group isolates were recovered (0.3 isolates per specimen). bacteroides fragilis accounted for 62% of the total of all b. fragilis group isolates, bacteroides thetaiotaomicron for 15%, bacteroides vulgatus for 8%, bacteroides ovatus for 7%, bacteroides distasonis for 6%, and bacteroides uniformis for 2%. of the 988 b. f ... | 1992 | 1393825 |
| pathogenicity of bacteroides fragilis group in rat intra-abdominal abscesses. | attempts were made to study the pathogenicity of some strains of bacteroides fragilis group in the rat intra-abdominal abscess model. multiple intraabdominal abscesses were produced in 50 to 70% of animals when an inoculum containing 10(9) cfu/ml of any of the five species of bacteroides fragilis group was injected. rising homologous antibody titers determined by indirect fluorescent antibody test were observed till the 3rd week when tested last, indirectly confirming the multiplication of the o ... | 1992 | 1479960 |
| 2,6-dichlorophenolindophenol is a competitive inhibitor for xanthine oxidase and is therefore not usable as an electron acceptor in the fluorometric assay. | xanthine oxidase has been recognized as an important source of oxygen free radicals in ischemia-reperfusion injury. in order to study this enzyme in biological tissues, the conversion of pterin (2-amino-4-hydroxypteridine) to isoxanthopterin provides the basis for a very sensitive fluorometric assay. xanthine oxidase is typically assayed in the presence of pterin only, while an electron acceptor which replaces nad+ is used to determine the combined xanthine dehydrogenase plus xanthine oxidase ac ... | 1992 | 1563644 |
| survey of bacteroides fragilis group susceptibility patterns in canada. | the in vitro activities of penicillin, clindamycin, chloramphenicol, metronidazole, piperacillin, piperacillin-tazobactam, ticarcillin, ticarcillin-clavulanate, ampicillin-sulbactam, cefoxitin, ceftizoxime, cefotetan, moxalactam, and imipenem against 348 bacteroides fragilis group isolates collected from six canadian cities during 1990 were determined by the national committee for clinical laboratory standards (nccls) agar dilution technique. all isolates were susceptible to chloramphenicol, met ... | 1992 | 1605600 |
| a novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. | acute and chronic experimental ulcerative colitis models were produced in mice by providing them with drinking water containing synthetic dextran sulfate sodium. mice that developed acute colitis showed signs of diarrhea, gross rectal bleeding, and weight loss within 6-10 days after ingesting 3%-10% dextran sulfate sodium. on postmortem examination, multiple erosions and inflammatory changes including crypt abscesses were found on the left side of the large intestine. mice that developed chronic ... | 1990 | 1688816 |
| the anti-tachycardic mechanism of a direct-acting vasodilator, budralazine, in rats. | the present study was undertaken to elucidate the anti-tachycardic effect of a direct-acting vasodilator, budralazine, using an electrophysiological technique. normotensive male wistar rats were used. rats were anesthetized intraperitoneally with urethane and alpha-chloralose. intravenous administration of budralazine (0.5-5.0 mg/kg) produced a dose-dependent reduction of mean arterial pressure in anesthetized rats. at doses of 0.5 and 1.0 mg/kg, budralazine induced bradycardia accompanied with ... | 1991 | 1773498 |
| the effect of ecdysteroids on the microfilarial production of brugia pahangi and the control of meiotic reinitiation in the oocytes of dirofilaria immitis. | the effects of the ecdysteroids ecdysone and 20-hydroxyecdysone on microfilarial release in brugia pahangi and on meiotic reinitiation in the oocytes of dirofilaria immitis were studied. ecdysone was found to stimulate microfilarial release at 2 x 10(-6) m, but this effect was reduced at higher and lower concentrations. 20-hydroxyecdysone was found to have no such effect. in d. immitis, ecdysone at 10(-5) m was also found to overcome the period of meiotic arrest that occurs during the pachytene ... | 1991 | 1994372 |
| epidemiology, antimicrobial susceptibility, pathogenicity, and significance of bacteroides fragilis group organisms isolated at los angeles county-university of southern california medical center. | the epidemiology of species of the bacteroides fragilis groups isolated at los angeles county-university of southern california medical center was examined. in addition, frequency of resistance to six beta-lactam antibiotics (cefmetazole, cefotetan, ceftizoxime, imipenem, penicillin, and cefoxitin) and to clindamycin, chloramphenicol, and metronidazole was determined for each species. while b. fragilis was most commonly isolated, the other species of the b. fragilis group accounted for half of t ... | 1991 | 2017610 |
| efficacy and safety of recombinant human erythropoietin to prevent the anaemias of prematurity. european randomized multicenter trial. | | 1991 | 2040194 |
| isolation and identification of strains of bacteroides fragilis group from the digestive tract of callithrix penicillata marmosets. | thirty-five strains of the bacteroides fragilis group were isolated from oral and intestinal samples from 5 wild caught, captive callithrix penicillata. nine oral strains of bacteroides fragilis (7) and bacteroides distasonis (2), and 26 intestinal strains of bacteroides fragilis (14) and bacteroides distasonis (12) were identified. | 1990 | 2106054 |
| [new antitubercular drugs]. | | 1990 | 2168041 |
| imipenem resistance in bacteroides distasonis mediated by a novel beta-lactamase. | imipenem is a highly active drug against the bacteroides fragilis group of organisms. on the basis of a nationwide survey of over 500 isolates, it was found that the frequency of imipenem resistance was less than 0.1%. we have a highly resistant bacteroides distasonis isolate, tal7860, for which the following mics (micrograms per milliliter) were determined by agar dilution: cefoxitin, greater than 128; moxalactam, greater than 128; piperacillin, greater than 128; imipenem, 16; and sch34343, 16. ... | 1990 | 2327745 |
| risk factors for kaposi's sarcoma in the vancouver lymphadenopathy-aids study. | in our ongoing cohort study of homosexual men, the ratio of new kaposi's sarcoma (ks) cases to new opportunistic infections (oi) during the periods 1982-1985, 1986-1987, and 1988-1989 fell from 0.75 (9 ks: 12 oi) to 0.57 (12 ks:21 oi) to 0.27 (4 ks:15 oi), respectively. to examine factors associated with the development of ks as compared to oi, we compared antecedent risk factors in 25 ks cases and 48 oi "controls." in univariate analyses, several classical hiv risk factors including numbers of ... | 1990 | 2395081 |
| [bacteroides distasonis meningitis]. | a 50 year old woman while undergoing severe treatment for rheumatoid arthritis, developed anaerobic meningitis. the cerebrospinal fluid (csf) sample was transported and cultivated aerobically and anaerobically. after 48 h at 37 degrees c the anaerobically incubated plate, the enriched fluid thioglycollate medium and the anaerobic culture medium yielded luxuriant growth of an anaerobic gram negative bacillum. the biochemical and antimicrobial susceptibility patterns were consistent with those for ... | 1989 | 2487744 |
| effects of serum testosterone level with buserelin on the activities of drug and testosterone hydroxylase and on the content of a male-specific form of cytochrome p-450 in male rats. | the effects of administration of buserelin, a synthetic agonist of lutenizing hormone-releasing hormone (lh-rh), on the content of p-450-male, a male specific form of cytochrome p-450, and the activities of drug and testosterone hydroxylases were examined in liver microsomes of male rats. administration of buserelin resulted in a decrease in the serum level of testosterone in a dose-dependent manner. similar decreases were seen in the activities of aminopyrine n-demethylase, 7-propoxycoumarin o- ... | 1989 | 2492807 |
| evidence that sindbis virus nsp2 is an autoprotease which processes the virus nonstructural polyprotein. | the four nonstructural proteins (nsp1-4) of sindbis virus, a member of the togaviridae family, are initially expressed from the 5' segment of the single-stranded genomic (+)rna as a polyprotein which is subsequently proteolytically processed. in attempts to identify the protease acting on this nonstructural polyprotein, we established a coupled in polyprotein, we established a coupled in vitro transcription-translation system which was able to faithfully process the major polyprotein when an mrn ... | 1989 | 2525839 |
| patterns of resistance to anaerobic organisms in australia. | this study investigated patterns of resistance in anaerobic organisms isolated at westmead hospital in sydney, australia, during the years 1987 to 1988. minimal inhibitory concentrations (mics) to ampicillin, sulbactam/ampicillin, metronidazole, clindamycin, and cefoxitin were determined by agar dilution for 200 anaerobes from clinically significant infections. antibiotics active against nearly all of these anaerobes included metronidazole and sulbactam/ampicillin, which demonstrated good activi ... | 1989 | 2591175 |
| omental autoimplantation of splenic tissue and intravenous pneumococcal challenge. a comparative study in rats. | splenectomized subjects show a higher incidence of myocardial ischemia and of overwhelming post-splenectomy infection (opsi). it is doubtful that implanted splenic tissue guarantees an adequate protection from opsi. the histological characteristics and the capacity of protection from opsi of two models of omental autoimplantation of splenic tissue in rats were examined. the implanted splenic tissue offers a significant protection from pneumococcal sepsis, even though there is no relation between ... | 1989 | 2628385 |
| pathogenicity of the bacteroides fragilis group. | the bacteroides fragilis group is one of the most important pathogens in polymicrobial infections. the distribution of the different members of the b. fragilis group in clinical infections varies. bacteroides fragilis accounts for 63 percent of all the group isolates, bacteroides thetaiotaomicron for 14 percent, bacteroides vulgatus and bacteroides ovatus for seven percent each, bacteroides distasonis for six percent and bacteroides uniformis for two percent. all members of the group induced bac ... | 1989 | 2679351 |
| falciparum malaria--present day problems. an experience with 425 cases. | clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ... | 1989 | 2693436 |
| design, expression, and crystallization of recombinant lectin from the garden pea (pisum sativum). | the propeptide form of the lectin from the garden pea (pisum sativum agglutinin) has been expressed in escherichia coli by attaching its cdna to an inducible promoter. by a number of criteria, including the ability to form dimers, hemagglutination titer, western blot, and enzyme-linked immunosorbent assay, the resulting propeptide molecule is virtually indistinguishable from the mature proteolytically processed lectin isolated from peas. preliminary crystallization experiments using the recombin ... | 1989 | 2708344 |
| design, expression, and crystallization of recombinant lectin from the garden pea (pisum sativum). | the propeptide form of the lectin from the garden pea (pisum sativum agglutinin) has been expressed in escherichia coli by attaching its cdna to an inducible promoter. by a number of criteria, including the ability to form dimers, hemagglutination titer, western blot, and enzyme-linked immunosorbent assay, the resulting propeptide molecule is virtually indistinguishable from the mature proteolytically processed lectin isolated from peas. preliminary crystallization experiments using the recombin ... | 1989 | 2708344 |
| antibody responses and protection in mice immunized with herpes simplex virus type 1 antigen immune-stimulating complex preparations. | the formation of immune-stimulating complexes (iscoms) obtained by mixing the glycoside quil a with an antigen preparation derived from herpes simplex virus type 1 (hsv-1)-infected cell cultures using a zwitterionic detergent is described. the hsv-1 antigen preparation incorporated into iscoms elicited significantly greater antibody responses in mice than the same preparation administered together with aluminium hydroxide gel, and provided complete protection against hsv-1 or hsv-2 lethal, syste ... | 1989 | 2769234 |
| biochemical properties of beta-lactamase produced by bacteroides distasonis. | three of 29 clinical isolates of bacteroides distasonis strains, gai2095, gai2361 and gai6270 were found to produce high levels of beta-lactamase relative to the remaining 26 strains. the enzymes from these strains showed a broad substrate profile, hydrolyzing cephaloridine, cephalothin, cefazolin, oxyimino-cephalosporins such as cefuroxime, cefotaxime and cefmenoxime, and piperacillin at high rates. examination of the substrate profiles indicated that the enzymes were mainly oxyimino-cephalospo ... | 1989 | 2786864 |
| antiviral chemoprophylaxis for cytomegalovirus infection. | | 1988 | 2849602 |
| future trends in the management of peptic ulcer disease. | future trends in the short- and long-term management of peptic ulcer disease are considered. the present state of development of pharmacologic agents for the short-term healing of duodenal and gastric ulcer is impressive, and high rates of healing with rapid symptom relief can be safely achieved with several agents. the problem of ulcer recurrence has not been solved, although new work concerning the role of campylobacter pylori holds promise. the mortality of acute gastrointestinal haemorrhage ... | 1988 | 2906462 |
| structure and expression of the hepatitis b virus genome. | | 1987 | 2948897 |
| [morphologic studies following coronary thrombolysis and ptca]. | compared with those of other species human coronary arteries show a particularly marked diffuse thickening of the intima. superimposed on this diffuse thickening local sclerotic changes develop. in early stages they contain plenty of lipids which are mainly stored in modified smooth muscle cells, partly also in cells of monocytic origin. normally ruptures of arteriosclerotic plaques are responsible for thrombotic occlusions in extensive transmural myocardial infarctions. they are caused by ruptu ... | 1986 | 2950674 |
| [clostridium butyricum and bacteroides distasonis peritonitis in a child treated by peritoneal dialysis. usefulness of anaerobic culture after filtration]. | | 1986 | 2954070 |
| activity of trospectomycin against bacteroides fragilis and other bacteroides species. | the in vitro activity of trospectomycin (u-63366; 6'-n-propyl spectinomycin pentahydrate sulfate) was evaluated against 189 clinical isolates of the bacteroides fragilis group and 65 bacteroides species isolates. at less than or equal to 8 micrograms/ml, the activity of trospectomycin compared favorably with those of clindamycin and cefoxitin against b. fragilis, bacteroides distasonis, and bacteroides vulgatus, and there was no cross resistance to these three drugs among the strains of the b. f ... | 1988 | 2967671 |
| intestinal bacterial flora and host defense mechanisms. | the protective mechanisms of intestinal bacterial flora against exogenous infections will be discussed in this paper. experimental data on protective function of escherichia coli, enterococcus faecalis and bacteroides distasonis comprising intestinal flora against oral infection of shigella flexneri which causes localized infection are presented. furthermore, the present investigation deals with non-specific defense mechanism which indicate that protective activity of intestinal flora against pa ... | 1986 | 2968005 |
| regulation by interleukin 2 of interleukin 2 receptors and gamma-interferon synthesis by human thymocytes: augmentation of interleukin 2 receptors by interleukin 2. | the role of interleukin 2 (il 2) on the expression of il 2 receptors and on the synthesis of gamma-interferon (gamma-ifn) by human thymocytes was investigated. human thymocytes isolated from specimens obtained during cardiac surgery of infants and children were induced with one or all of the following agents: il 2, concanavalin a (con a), and 12-o-tetradecanoylphorbol 13-acetate (tpa). the expression of il 2 receptors and gamma-ifn titers were determined. the results indicate that thymocytes cul ... | 1985 | 2981271 |
| demonstration of non-degraded aleutian disease virus (adv) proteins in lung tissue from experimentally infected mink kits. brief report. | isolates of adv replicate to rather high quantities in lungs from neonatally infected mink kits. the virus was analysed for polypeptide composition, and for the first time high molecular weight polypeptides have been observed in in vivo produced advs. these polypeptides are analogous to those of in vitro produced advs. the molecular weights of the structural polypeptides of the low virulence pullman adv and the highly virulent dk and utah i isolates of adv were found to be 88kd and 78kd and in v ... | 1986 | 3002299 |
| gestational trophoblastic diseases: the ratio of choriogonadotropin to specific pregnancy protein, hcg/sp1, provides useful diagnostic and prognostic evidence. | serum levels of human chorionic gonadotropin (hcg), specific pregnancy protein (sp1), and hpl were measured in 675 samples from women with uneventful pregnancy, and serially from the time of presentation in 125 patients with hydatidiform mole (hm), 43 with invasive mole (im), and 34 with choriocarcinoma (cc). in hm serum levels of hcg and sp1 declined steadily from presentation to remission; when gestational age at the time of molar evacuation was shorter than 11 weeks, hcg declined to the norma ... | 1986 | 3002918 |
| central nervous system lesions in suckling mice and rats inoculated intranasally with sialodacryoadenitis virus. | suckling cd-1 and cfw mice and wi wistar rats were inoculated intranasally with sialodacryoadenitis virus. in animals inoculated during the first week of life, there was an acute necrotizing encephalitis with malacia and minimal inflammatory cell response. in mice and rats which survived for up to 12 days post inoculation, loss of brain substance, gitter cells and occasionally mineralized debris were seen. viral antigen was readily demonstrated in neurons and nasal epithelium in trypsin-treated, ... | 1986 | 3004003 |
| [morphological changes caused by adrenalectomy in rabbits]. | | 1986 | 3019628 |
| [morphological changes caused by adrenalectomy in rabbits]. | | 1986 | 3019628 |
| sulfite oxidase from chicken liver. the role of imidazole and carboxyl groups for the reaction with cytochrome c. | oxidation of sulfite to sulfate by sulfite oxidase is inhibited when the enzyme is treated with reagents known to modify imidazole and carboxyl groups. modification inhibits the oxidation of sulfite by the physiological electron acceptor cytochrome c, but not by the artificial acceptor ferricyanide. this indicates interference with reaction steps that follow the oxidation of sulfite by the enzyme's molybdenum cofactor. reaction with diethylpyrocarbonate modifies ten histidines per enzyme monomer ... | 1986 | 3019695 |
| sulfite oxidase from chicken liver. the role of imidazole and carboxyl groups for the reaction with cytochrome c. | oxidation of sulfite to sulfate by sulfite oxidase is inhibited when the enzyme is treated with reagents known to modify imidazole and carboxyl groups. modification inhibits the oxidation of sulfite by the physiological electron acceptor cytochrome c, but not by the artificial acceptor ferricyanide. this indicates interference with reaction steps that follow the oxidation of sulfite by the enzyme's molybdenum cofactor. reaction with diethylpyrocarbonate modifies ten histidines per enzyme monomer ... | 1986 | 3019695 |
| localization of quantitative changes in pulmonary beta-receptors in ovalbumin-sensitized guinea pigs. | impaired beta-receptor function has been postulated as one factor contributing to airway hyperreactivity in asthmatic patients. although numerous indirect studies have cast doubt on this theory, none of these previous investigations has been able to directly measure changes in beta-receptor number on intrapulmonary structures capable of affecting the physiologic changes seen in this disease state. to help clarify the intrapulmonary location of such changes, a model of allergic bronchoconstrictio ... | 1987 | 3037954 |
| natural killer cells are deficient in the surface expression of the complement regulatory protein, decay accelerating factor (daf). | decay-accelerating factor (daf) is a 75,000 m.w. membrane protein that inhibits autologous complement c3 activation at the cell surface. one-color direct immunofluorescence with anti-daf antibody and cytofluorographic analysis indicates that normal human monocytes and granulocytes are uniform in expression of daf, whereas 23% of peripheral blood lymphocytes are daf deficient. a two-color indirect immunofluorescence method, used to define the phenotype(s) of the daf-deficient lymphocytes, was les ... | 1986 | 3090145 |
| stimulatory and inhibitory regulation of myocardial adenylate cyclase by 5'-guanylyl-imidodiphosphate. | particulate and soluble rat myocardial adenylate cyclase enzymes were characterized with respect to their stimulatory and inhibitory regulation by gpp(nh)p. gpp(nh)p (60 microm) stimulated mg2+- and mn2+-dependent adenylate cyclase. high concentrations of gpp(nh)p (600 microm) attenuated the maximal stimulatory response to gpp(nh)p but only at low cation concentrations. the attenuating effects of 600 microm gpp(nh)p resulted predominantly from the introduction of a prolonged lag in the kinetics ... | 1987 | 3103629 |
| the inhibition by valproic acid of the mitochondrial oxidation of monocarboxylic and omega-hydroxymonocarboxylic acids: possible implications for the metabolism of gamma-aminobutyric acid. | the interactions of 1-5 mm valproic acid with the hepatic fatty acid oxidation are here described. valproic acid was not substrate for hepatic peroxisomal fatty acid oxidation. its activation outside the mitochondrial matrix compartment was poor when compared to that of octanoic acid, a fatty acid containing the same number of carbones. valproic acid did not inhibit the fatty acyl-coa oxidase nor the cyanide-insensitive acyl-coa oxidation. valproic acid inhibited the mitochondrial oxidations of ... | 1987 | 3117781 |
| [feed-dependent changes in the rumen mucosa of high-producing cows from the dry period to eight weeks post partum]. | | 1987 | 3122467 |
| intravenous immune globulin in symptomatic paediatric human immunodeficiency virus infection. | seven paediatric patients with symptomatic human immunodeficiency virus (hiv) infection were prospectively studied for 6-24 months after the start of intravenous immune globulin (ivig) therapy. there was substantial clinical benefit during ivig treatment with marked reduction of febrile and infectious episodes, normalization of physical and psychomotor development, and absence of mortality. the immunologic monitoring revealed some discrete objective improvements. the results of this study favour ... | 1988 | 3134244 |
| ultrasonic assessment of cardiac function in the intact human fetus. | echocardiography may provide information concerning the structural development of the fetal human heart. the information available from such studies, when interpreted in light of existing knowledge of developmental cardiac physiology, may give insight concerning in utero cardiac pump function. the quantitation of cardiac structural growth in utero has been used to provide growth curves for the chambers of the fetal heart. disparity in the ratio of right ventricular/left ventricular size (normall ... | 1985 | 3155529 |
| comparative in vitro beta-lactam activity against aerobic and anaerobic surgical isolates. | the comparative in vitro activity of cefoxitin, piperacillin, cefotetan, and ceftizoxime was compared against 843 surgical microbial isolates. all compounds exhibited excellent activity against the streptococcal isolates. antimicrobial activity was poor for staphylococcus aureus and s. epidermidis. cefotetan activity against the enterobacteriaceae was comparable to ceftizoxime (greater than 95% susceptible). resistance rates of 0, 1, 5, and 2% were observed with cefoxitin, piperacillin, cefoteta ... | 1988 | 3164263 |
| blastocystis hominis: clinical and therapeutic aspects. | | 1988 | 3176148 |
| subendocardial and subepicardial wall thickening during ischemia in exercising dogs. | to determine whether ischemia in the exercising dog is associated with preservation of subepicardial thickening relative to subendocardial thickening, 10 dogs were chronically instrumented with circumflex artery flow probes, hydraulic occluders, and pairs of ultrasonic microcrystals for determination of wall thickness in the circumflex artery distribution. one pair of crystals spanned the entire ventricular wall (transmural), and the other spanned the outer half of the ventricular wall. inner wa ... | 1988 | 3180383 |
| influence of direct currents on bone vascular supply. | electrical stimulation with a dc of 5 microa was found to result in macromolecular leakages in the microcirculation of rabbit metaphyseal bone. the blood flow rate of living bone did not change during 2.5 hours of electrical stimulation. over a follow-up time of up to 11 weeks it was observed that vessels close to bone borders increased in size and that the number of bone capillaries increased, simultaneous with an increase in bone formation. | 1988 | 3187445 |
| deviations in hepatic amino acid profiles of mouse following repeated hexachlorophene administration. | effects of repeated administration of hexachlorophene (hcp) on the food consumption, body and liver weights, liver somatic indices and hepatic levels of the free amino acids and amino acid-enzyme activities have been studied. a dose-dependent reduction in body weights and food consumption and increased liver somatic indices suggested a progressive and on toward response to hcp. the concentrations of hepatic free amino acids: phenylalanine, tyrosine, leucine, isoleucine, valine, threonine, aspara ... | 1988 | 3192908 |
| neuropathological definition of alzheimer disease: multivariate analyses in the morphometric distinction between alzheimer dementia and normal aging. | although establishing that a patient is suffering from dementia of the alzheimer type initially reflects a clinician's opinion, neuropathological study is for the present the most definitive examination to confirm the clinical diagnosis of alzheimer disease. we review several comprehensive publications attempting on a quantitative basis to differentiate the changes occurring with normal aging of the human brain from those indicative of alzheimer disease. new morphometric data on 5 histopathologi ... | 1988 | 3207478 |
| [phase i clinical study of 99mtc-dtpa-human serum albumin]. | | 1988 | 3225966 |
| individual free fatty acids and lactate uptake in the human heart during severe sepsis. | coronary haemodynamics and myocardial metabolism of nonesterified fatty acids (nefa) and lactate were studied in 11 patients with severe sepsis, and compared to 10 control subjects. coronary sinus blood flow was evaluated by thermodilution. arterial and coronary sinus blood samples were collected for the measurement of lactate and total and individual nefa concentrations both in septic and control patients. there was an increase in lactate and total nefa arterial concentrations with a marked inc ... | 1988 | 3232957 |
| a second chain of human cd8 is expressed on peripheral blood lymphocytes. | human cd8 has been thought to consist of disulfide-linked homodimers and homomultimers of a single polypeptide chain homologous to mouse and rat cd8 alpha. in contrast, mouse and rat cd8 are composed of disulfide-linked heterodimers of alpha and beta chains. we have now isolated and sequenced cdna clones encoding a human homologue of mouse and rat cd8 beta. one such clone was inserted into an expression vector and its encoded product was shown to be expressed on the cell surface after cotransfec ... | 1988 | 3264320 |
| criteria for in vitro susceptibility testing of cefotetan. correlations with clinical and bacteriologic responses. | standardized in vitro susceptibility testing criteria and quality-control guidelines for cefotetan have been well documented in the scientific literature. cefotetan and cefoxitin differ in their in vitro spectrums of activity and cannot be used interchangeably for in vitro tests. in vitro susceptibility information failed to correlate with clinical or bacteriologic responses of treated patients when 314 anaerobic bacteria from 145 patients were evaluated. all cefotetan-resistant anaerobic bacter ... | 1988 | 3287965 |
| [medico-genetic studies of human provisional organs]. | | 1987 | 3318529 |