| [intratypical antigenic differentiation of poliovirus strains circulating in mali by means of cross-adsorbed sera]. | the intratypic antigenic differentiation of poliovirus strains isolated from sick or healthy children in mali in 1975-1976 was performed. the method is based on the use of cross-adsorbed sera in the neutralization tests in which the virus is titrated by the plaque procedure or by the cytopathic effect. all the examined strains of serologic types i, ii, and iii were distinguished by the antigenic structure from the vaccine strains and were classified as "wild". quantitative differences in the ant ... | 1978 | 86237 |
| the use of trypan blue for counterstaining in the sepharose bead immunofluorescence test. the application of the test for the demonstration of primate retrovirus-specific antibodies and antigens. | the sepharose bead immunoflurorescence test was performed by counterstaining the beads with trypan blue. this results in a red staining of negative beads which allows an easy distinction from positive green-fluorescent beads. sepharose beads conjugated with viral proteins or antiviral antibodies were used to demonstrate mason-pfizer monkey virus (mpmw)- and simian sarcoma virus (ssv) - specific antigens or antibodies. the test shows a high sensitivity and specificity and needs a small amount of ... | 1978 | 86253 |
| antibodies detectable by counterimmunoelectrophoresis against bacteroides antigens in serum of patients with inflammatory bowel disease. | heat-extracted antigens from seven species of bacteroides were used in passive hemagglutination and counterimmunoelectrophoretic tests. sera from 87 normal persons (group i) and 15 patients with ulcerative colitis (group ii) were of low and equal reactivity in passive hemagglutination tests; all positive tests were eliminated by 2-mercaptoethanol reduction of the sera. when these same sera were tested by counterimmunoelectrophoresis with six of the bacteroides antigens, no significant difference ... | 1979 | 88458 |
| the pathophysiology of asthma. | because postmortem studies of humans provide little information on the initial pathophysiologic events in asthma, animal models have been developed. recently the ascaris-allergic rhesus monkey has provided an opportunity to examine the onset of pathophysiologic changes following challenge and to correlate them with airway structure. these studies have suggested that the initial interaction between antigen and mast cells may occur in the bronchial lumen or in the epithelium superficial to the tig ... | 1979 | 89006 |
| histamine-releasing effect of a corticotrophin derivative. ii. mechanism of action of histamine release by c 44 680-ba, compared with that of cpd. 48/80, dextran and triton. | the mechanism of the histamine-liberating action of the synthetic polypeptide c 44 680-ba, an alkyl-prolyl derivative of beta 1-19 corticotrophin, was investigated and compared with those of compound 48/80, dextran, melittin and triton x-100. it was found that the release of histamine from rat peritoneal cells induced by the polypeptide is dependent on temperature, ph, calcium ions and energy-providing processes. in regard to these criteria, the mode of action of this histamine liberator resembl ... | 1979 | 89804 |
| distribution of myosin isoenzymes among skeletal muscle fiber types. | using an immunocytochemical approach, we have demonstrated a preferential distribution of myosin isoenzymes with respect to the pattern of fiber types in skeletal muscles of the rat. in an earlier study, we had shown that fluorescein-labeled antibody against "white" myosin from the chicken pectoralis stained all the white, intermediate and about half the red fibers of the rat diaphragm, a fast-twitch muscle (gauthier and lowey, 1977). we have now extended this study to include antibodies prepare ... | 1979 | 90047 |
| the use of avidin-biotin interaction in immunoenzymatic techniques. | biotin was covalently attached to antibodies, antigens and enzymes, and the effects of this labeling on the antigen and antibody binding capacity and on enzymatic activity were tested. based on avidin-biotin interaction, the labeled proteins were used in quantitative enzyme-immunoassay and enzyme-immunohistochemical staining procedures. two procedures were developed. in the first procedure, named the bridged avidin-biotin (brab) technique four steps were used sequentially in order to quantify or ... | 1979 | 90074 |
| polyribonucleotide inhibition of ribonucleic acid directed deoxyribonucleic acid polymerase of mouse mammary tumor (type b) virus and simian sarcoma (type c) virus. | | 1979 | 90331 |
| the expression of a human b-lymphocyte antigen and surface membrane immunoglobulin by lymphoid cells from patients with lymphocytic lymphoma, multiple myeloma and benign monoclonal gammapathy. | | 1979 | 90449 |
| the intra-nucleolar localization of amplified rdna in xenopus laevis oocytes. | a fluorescent feulgen-stain was adapted in order to demonstrate dna-containing structures inside the amplified nucleoli of xenopus laevis. at all stages of oogenesis this method reveals granules or complex structures of dna in each nucleolus. the micronucleoli which do not stain with this method and which do not reveal an internal structure in low molarity saline, unlike real nucleoli are considered as nucleolus-like bodies. the dna-containing structures in the nucleoli can be composed of one or ... | 1979 | 90588 |
| antigens in penicillin allergy. iv. induction of ige antibody response in mice after daily treatment with contaminated but not with pure penicillin. | | 1979 | 90657 |
| characterization of a type-c virus produced by co-cultures of human leukemic bone-marrow and fetal canine thymus cells. | the putative human helper virus ska-21/a204v, isolated by nooter et al. in 1977 from human leukemic bone-marrow cells following co-culture with normal fetal canine thymus cells, cf2th, has been characterized with respect to its major viral core protein, reverse transcriptase, and nucleic acid sequences. the results of these analyses show that this virus is not distinguishable from the woolly monkey type-c virus, ssav-1, by the techniques employed. | 1979 | 90662 |
| purification of a new high activity form of glucose-6-phosphate dehydrogenase from rat liver and the effect of enzyme inactivation on its immunochemical reactivity. | a new form of cytoplasmic glucose-6-phosphate dehydrogenase (e.c.1.1.1.49) was purified from rat liver by protamine sulfate precipitation, ammonium sulfate fractionation, ion exchange chromatography with diethylaminoethyl cellulose, and affinity chromatography with cibacron blue agarose and nadp agarose. this form of the enzyme has a specific activity of over 600 units/mg of protein and gives essentially a single band by polyacrylamide gel electrophoresis. the form of the enzyme isolated by this ... | 1979 | 90677 |
| radioprotection of mouse intestine by inhibitors of cyclic amp phosphodiesterase. | | 1979 | 91600 |
| binding of purified, soluble major histocompatibility complex polypeptide chains onto isolated t-cell receptors. i. reactivity against allo- and self-determinants. | in this study, we tried to get information about the fine antigen-binding ability of purified, soluble, idiotype-positive t-cell receptor molecules. lewis anti-da t-cell receptors were purified from normal lewis serum by the use of anti-idiotypic immunosorbent and sodium dodecyl sulfate-polyacrylamide gel, and were coupled to cyanogen bromide-activated sepharose 4b. in parallel, lewis anti-da, lewis anti-bn, and da anti-lewis alloantibody immunosorbents were prepared. the major ag-b chain (44,00 ... | 1979 | 91653 |
| trace polypeptides in cellular extracts and human body fluids detected by two-dimensional electrophoresis and a highly sensitive silver stain. | development of a highly sensitive silver stain permits the characterization of trace cellular and body fluid proteins separated by the two-dimensional electrophoresis technique of o'farrell. many of the proteins detected by the silver stain in urine, spinal fluid, amniotic fluid, and cells were undetected with the widely used coomassie blue stain. trace polypeptides observed in escherichia coli cell lysates with this silver stain could be detected previously only by growth in radioactive precurs ... | 1979 | 92027 |
| electron-microscopic identification of axonal initial segments belonging to interneurons in the dorsal lateral geniculate nucleus of the monkey. | | 1978 | 98733 |
| [some aspects relating to the aflatoxin generation during the self-heating of cereals]. | in a stored batch of grain which was already affected by mould-formation tests were carried out with the known aflatoxin producer aspergillus flavus. a significantly lower aflatoxin production ensued if the mould growth was not connected with self-heating of the stored product. however, in conformity with increasing mould formation the germinating power was adversely affected and the significant signs (fatty acid number, reductive and none-reductive sugars) were influenced in the grain, irreleve ... | 1978 | 98933 |
| [isolation of treponemas from the colon of pigs with clinical dysentery]. | optimal culture conditions in artificial nutritive media were determined for a defined avirulent strain of treponema hyodysenteriae and for four field strains of treponemas in pigs with clinical dysentery. the treponemas were isolated with the use of milliporous filters with pores of 0.3 micrometer in diameter, which were located on the surface of blood agar. no significant difference in the influence of equine, bovine or sheep blood on the growth of treponemas was determined. the commercial amo ... | 1979 | 105448 |
| multiple sclerosis: immunochemical studies on the demyelinating serum factor. | sera from patients with multiple sclerosis (ms) frequently produce demyelination of central nervous system tissue cultures. the nature of the factors responsible for demyelination is not as yet clearly established. however, several authors previously reported, in in vivo and in vitro models, demyelinating activity in igg fractions isolated from sera and cerebrospinal fluid of ms patients14,42,48. we found the demyelinating activity of ms sera to be extremely labile to conventional biochemical tr ... | 1979 | 105780 |
| betamethasone and the rhesus fetus: multisystemic effects. | in this controlled study of betamethasone administration to pregnant rhesus monkeys, using dosages per gram of fetal body weight similar to those reported in several human clinical studies, the most significant fetal pulmonary changes observed were increases in maximum lung volumes. the fact that comparable increases in peak volumes were demonstrated on saline filling supports our contention that these changes are related primarily to lung structural alterations rather than surfactant effects. a ... | 1979 | 106730 |
| effects of trh and ergocryptine on mammary arterial and venous concentrations of growth hormone in lactating cows. | | 1979 | 107518 |
| [new species of microsporidians (microsporidia, nosematidae) from horsefly larvae in the ukraine]. | a new species of microsporidians, stempellia lairdi sp. n., was found in larvae of tabanus autumnalis l. from ukraine. it affects the muscular tissue and haemolymph of horse fly larvae. spores of the species are oval, egg-shaped, 1.8--7.6 x 1.2--3.8 mu in size. the species is pathogenic and causes the mortality of infected larvae. | 1979 | 108652 |
| protein structure refinement: streptomyces griseus serine protease a at 1.8 a resolution. | | 1979 | 119870 |
| neonatal low-level lead exposure in monkeys: locomotor activity, schedule-controlled behavior, and the effects of amphetamine. | | 1979 | 120039 |
| [pseudomonas aeruginosa--the causative agent of infection in birds]. | pseudomonas aeruginosa was isolated from the viscera of diseased and dead birds (young and adult chickens) as well as from 9 poultry houses on seven farms. its typing was performed on the basis of the blue-green coloration of the cultures, their hemolytic activity, the pleasant odour of lime-tree, the growth in culturing at 42 degrees c, the presence of the pyocianin pigment, and the positive results of the cytochromoxidase test after gaby and hadley and the biochemical test after kovacs. the pa ... | 1975 | 127412 |
| [human genetics: a task for the public health service? (author's transl)]. | | 1977 | 143637 |
| interaction of lysine-rich histone and dna in chromatin structure. | the heat denaturation and renaturation curves of rat liver and ascites hepatoma (ah 108a) chromatins were measured. in these renaturation curves, there are small sigmoidal regions. these sigmoidal regions remained in redenaturation curves and were largely stable to dnaase i digestion. when the chromatins were treated stepwise with naclo4 and lysine-rich histones were removed, the sigmoidal regions in the renaturation curves disappeared. these results suggested that the sigmoidal regions reflecte ... | 1975 | 167842 |
| a survey of intestinal parasitism in a community on the pan american highway route in eastern panama. | a survey of intestinal parasitism was conducted at yaviza, a town in eastern panama's province of darien. single stool specimens from 202 persons, representing 11 percent of the town's total population, were examined. in all, 181 (90 per cent) of the specimens examined were found positive for one or more potentially pathogenic parasites. the most common parasite found was trichuris trichiura, which was observed in 80 per cent of the samples. others were ascaris lumbricoides, in 62 per cent of th ... | 1975 | 167889 |
| steroid production by quartered and capsular rat adrenal gland in response to haemorrhage. | 60 min after rapid bleeding (1.5--2.0 per cent of b. w.) both aldosterone and corticosterone production rate by quartered rat adrenals were found to be elevated. however, no difference was observed in the rate of aldosterone and corticosterone production by capsular adrenals of sham operated and hypovolaemic rats. corticosterone production rate by decapsulated adrenals was much more higher after haemorrhage than in the control group. the same alterations could be observed incubating adrenal tiss ... | 1975 | 179800 |
| the protective action of disodium cromoglycate on alveolar macrophages in phagocytosis of fibrogenic silica. | | 1976 | 187887 |
| hydrolysis of nicotinamide adenine dinucleotide by choleragen and its a protomer: possible role in the activation of adenylate cyclase. | choleragen and the isolated a protomer catalyzed the hydrolysis of nad to adp-ribose and nicotinamide. the protein with nadase activity (nad nucleosidase; nad glycohydrolase, ec 3-2-2-5) migrated on polyacrylamide gels with choleragen, and chromatographed on bio-gel p-60 columns with the a protomer. the nadase activity of choleragen and of the a protomer was increased markedly in acetate and phosphate buffers, and enhanced over 10-fold by dithiothreitol in high concentration. nad hydrolysis was ... | 1976 | 188038 |
| [effects of the nervous system on the kidney. part i. review of the literature]. | the authors review the literature on the effects of the nervous system on the kidney in 3 stages: 1) historical stage when the role of the renal nerve supply was overetimated by claude bernard. 2) intermediate stage between the two wars with a clinical description of uremic encephalits or acute delirium. from the experimental point of view was demonstrated the inessential character of the nerve supply for the survival of the kidney. 3) modern stage: from the clinical point of view one may distin ... | 1976 | 188168 |
| localization and activity of tissue bound cyclic nucleotide phosphodiesterase in normal and lack of changes in psoriatic human skin. | this study has been undertaken to elucidate the localization and the activity of cyclic nucleotide phosphodiesterase (pde) in psoriatic epidermis compared to normal. the results showed that the evaluation of cytochemical methods may be difficult because of the various factors which interfere with the reaction and the considerable amount of background staining. additionally, only the tissue bound particulate enzyme fraction may be demonstrated by cytochemical means. nevertheless, the method did r ... | 1976 | 188439 |
| [isolation of preparations of type b monoamine oxidase from liver mitochondria]. | | 1977 | 193582 |
| ovarian involvement by cytomegalovirus. | | 1978 | 204571 |
| characterization of a murine myeloma (mopc-315) c-type virus by nucleic acid hybridization. | | 1979 | 219600 |
| activity of semisynthetic penicillins and synergism with mecillinam against bacteroides species. | the minimal inhibitory concentrations (mic) of six penicillins (ampicillin, carbenicillin, ticarcillin, piperacillin, mezlocillin, and bay k 4999) against 29 clinical isolates of bacteriodes spp. (including bacteroides fragilis, bacteroides thetaiotaomicron, and bacteroides vulgatus) were determined by an agar dilution method. bay k 4999 was most active, followed in descending order by ampicillin, piperacillin, mezlocillin, ticarcillin, and carbenicillin. mecillinam, a 6 beta-amidino-penicillani ... | 1979 | 228593 |
| decline of maternal antibodies to plague in norway rats. | the decline of maternal antibodies to the fraciton i antigen of yersinia pestis was investigated in newly weaned rattus norvegicus obtained from dams vaccinated with strain ev76(51f) of y. pestis. iha titre decreased by 50% each 7-3 days and cf titre declined 50% each 10-0 days in young rats. an analysis of available data indicated that maternal iha and cf antibodies could persist to 3 months of age. therefore, positive serologic reactions in young r. norvegicus, detected in the course of serolo ... | 1977 | 264498 |
| nonrandom chromosome rearrangements in 27 cases of human myeloid leukemia. | the r-banding pattern of the chromosomes of 31 patients hospitalized in the hematologic clinic for myeloid leukemia were studied before chemotherapy. this analysis permitted identification of one unusual 3-chromosome rearrangement t(3;9;22) in addition to 25 classic forms of (22q-;9q+) translocation accompanied by the specific ph' chromosome in chronic granulocytic leukemia patients, independent of the blastic course of the disease. during blastic crisis observed in 6 patients, extra 8 and 10 ch ... | 1977 | 271632 |
| genetic and environmental factors in the etiology of dental disease in the aleut. | | 1977 | 273033 |
| comparison of primary and secondary stimulation of male rats by estradiol in terms of prolactin synthesis and mrna accumulation in the pituitary. | male rats received acute or chronic primary or acute secondary stimulation with estradiol, and the effects on pituitary prolactin synthesis and its mrna accumulation were examined. prolactin synthesis was determined by the in vitro incorporation of [(3)h]leucine into prolactin over a period of 1 hr. prolactin mrna was measured both by cell-free translation in a nuclease-treated rabbit reticulocyte lysate and by hybridization to the complementary dna. the latter two methods gave similar results u ... | 1979 | 284404 |
| demonstration of thy-1 antigen on pluripotent hemopoietic stem cells in the rat. | three approaches were used to demonstrate the presence of thy-1 antigen on the surface of pluripotent hemopoietic stem cells in the rat. in the first, stem cells from fetal liver, neonatal spleen, and adult bone marrow were prevented from forming hemopoietic colonies in the spleens of irradiated recipients spleen (colony-forming unit assay) by incubation with antibodies to thy-1 antigen. highly specific rabbit heteroantiserum to purified rat brain thy-1 antigen and mouse alloantisera to thy-1.1- ... | 1978 | 309914 |
| the effects of additional flora on the response of salmonella mutants lodged in the gastrointestinal tract. | a histidine auxotroph of salmonella typhimurium, strain ta1538, will lodge for several months in the gastrointestinal tract of otherwise germ-free rats and of rats additionally associated with bacteria characteristic of the normal flora such as lactobacillus plantarum and bacteroides vulgatus. in the presence of the additional flora, the concentration of strain ta1538 is diminished in the stomach but not in the lower gastrointestinal tract or in the feces. following the ingestion of 2-nitrofluor ... | 1977 | 318921 |
| superoxide dismutase in bacteroides fragilis and related bacteroides species. | superoxide dismutase (sod) activity was demonstrated in cell-free extracts of bacteroides fragilis, bacteroides vulgatus, bacteroides distasonis, bacteroides ovatus, and bacteroides thetaiotaomicron. the strains were grown under anaerobic conditions in trypticase soy broth, and the specific activity of sod in the extracts was, in most strains, higher than in cell-free extracts of escherichia coli b grown under anaerobic conditions. isoelectric focusing of the extracts in polyacrylamide gel demon ... | 1977 | 332713 |
| use of ganglioside affinity filters to identify toxigenic strains of clostridium botulinum types c and d. | clostridium botulinum neurotoxin is synthesized by toxic clones grown anaerobically on ganglioside affinity filters. the toxin binds to the filters and is detected by reaction with 125i-immunoglobulin g from type-specific antitoxin. toxin spots from culture filtrates were similarly identified. the c. botulinum type c and d strains were selected for developing this affinity filter assay because synthesis of the c1 and d toxins is bacteriophage dependent. toxigenic clones were distinguished from p ... | 1979 | 387601 |
| incidence and pathological features of spontaneous tumors in athymic nude mice. | systematic observation of 1141 nude mice (swiss background [corrected] strain) that received human tumor transplants revealed 24 spontaneous tumors, 18 of lymphoreticular origin and 6 pulmonary adenomas. spontaneous tumors were seen at an average age of 9.1 months, and 22 of the tumors were seen only in that fraction of our group (324 mice) surviving for 5 months or more (22 of 324 x 100 = 6.8%). transplantation of these tumors to other nude mice was successful in three of five cases. mice trans ... | 1979 | 427772 |
| [bacteriological and clinical studies on metronidazole in bacteroides fragilis infections (author's transl)]. | the antimicrobial activity of 24 bacteroides fragilis, 13 bacteroides thetaiotaomicron and 5 bacteroides vulgatus, all of which were recent clinical isolates, was determined by the agar dilution technique. these three species had similar susceptibility patterns, but the b. thetaiotaomicron strains were generally less susceptible than the b. fragilis strains. clindamycin was very active against 81% of the bacteroides strains at 3.1 mcg/ml of less while 21% of the bacteroides strains had mics of 1 ... | 1979 | 439390 |
| [importance of bilirubinuria in the diagnosis of viral hepatitis]. | | 1979 | 452553 |
| studies on selective induction of lysosomal enzyme activities in mouse peritoneal macrophages. | | 1979 | 522087 |
| pathogenic synergism between treponema hyodysenteriae and other selected anaerobes in gnotobiotic pigs. | gnotobiotic pigs were orally exposed to various anaerobes at 6 to 9 days of age and similarly inoculated with treponema hyodysenteriae b204 3 to 6 days later. watery diarrhea and fecal excretion of large quantities of mucus and some fibrin clots were observed 4 to 20 days after inoculation with b204 if other anaerobes were present. colonic lesions characteristic of swine dysentery were observed when b204 was present with fusobacterium necrophorum, three strains of bacteroides vulgatus, a clostri ... | 1979 | 528047 |
| synthesis of alpha-ketoglutarate by reductive carboxylation of succinate in veillonella, selenomonas, and bacteriodes species. | evidence for reductive carboxylation of succinate to synthesize alpha-ketoglutarate was sought in anaerobic heterotrophs from the rumen and from other anaerobic habitats. cultures were grown in media containing unlabeled energy substrates plus [14c]succinate, and synthesis of cellular glutamate with a much higher specific activity than that of cellular asparate was taken as evidence for alpha-ketoglutarate synthase activity. our results indicate alpha-ketoglutarate synthase functions in selenomo ... | 1979 | 533772 |
| swine dysentery: studies of gnotobiotic pigs inoculated with treponema hyodysenteriae, bacteroides vulgatus, and fusobacterium necrophorum. | transmission experiments were carried out in gnotobiotic pigs to determine whether lesions typical of swine dysentery could be produced by oral inoculation of treponema hyodysenteriae in combination with bacteroides vulgatus or fusobacterium necrophorum, or both. each of the organisms had been isolated from swine with early lesions of the disease. lesions were not found in 6 pigs inoculated with t hyodysenteriae alone, in 4 pigs given f necrophorum and t hyodysenteriae, or in 4 pigs given b vulg ... | 1978 | 624669 |
| factors affecting production of catalase by bacteroides. | several variables affected the production of catalase by members of the "bacteroides fragilis group" of anaerobic bacteria. both media yielded higher catalase levels than the respective agar media. addition of hemin to media after autoclave sterilization, rather than before, significantly increased production of catalase. both of these variables could be related to the available hemin concentration present in the medium being tested. significantly higher amounts of hemin were required for catal ... | 1978 | 730827 |
| [in vitro susceptibility of bacteroidaceae to cefoxitin and cephalothin (author's transl)]. | the activity of the new cephamycin c derivatives cefoxitin against 102 clinical isolates of bacteroidaceae (95 bacteroides strains and 7 fusobacterium-sphaerophorus cultures) was determined by tube dilution and standardized agar diffusion tests and compared to that of cephalothin. cefoxitin was more active than cephalothin against 56 bacteroides fragilis strains, 19 bacteroides thetaiota-omicron strains and two bacteroides splanchnicus strains. the difference seemed less pronounced with five bac ... | 1977 | 856727 |
| new approach to caloric stimulation of the vestibular receptor. | a new caloric testing method is described. during continuous aural irrigation, fluid is switched between hot and cold values at times computed according to a mathematical model of heat conduction in the labyrinth area. as a result, the induced temperature difference across the lateral semicircular canal describes an approximately sinusoidal time course, reaching peak values of equal magnitude but opposite sign. the magnitude of the caloric stimulus may be selected by choosing appropriate irrigat ... | 1975 | 1081359 |
| [studies on the protein and amino acid metabolism of laying hens using 15n labeled casein. 15n concentration in the intestinal content and intestinal tissue in relation to the time of the last 15n administration]. | four laying hens with anus praeternaturalis were fed 15n casein for a period of 6 experimental days. each experimental bird thus received a 15n excess (15n') of 154.44 mg. 11.54 mg of 15n' were found in lysine 3.99 mg in histidine and 9.58 mg in arginine. 2 hens were slaughtered 48 hours after the last 15n dose; another 2 were sacrificed after 144 hrs. in this investigation the n content of the intestinal contents and in the proteins of the small and large intestine were determined. the average ... | 1975 | 1233975 |
| albumin synthesis and effect of betamethasone on albumin synthesis in perfused liver of normal and ccl4-intoxicated rats. | synthesis of plasma albumin was studied in perfused liver of normal and ccl4-intoxicated rats, using 14c-bicarbonate method. 1. in normal liver, albumin synthesis averaged 5.6 mg/hr/100 g body weight. 2. in ccl4-intoxicated liver, albumin synthesis decreased to 4.6 mg at acute stage, but restored to 5.1 mg at non-septal fibrotic stage. at septal fibrotic, cirrhotic and cirrhotic stage with ascites, the synthesis rate averaged 4.5 mg, 4.1 mg and 3.2 mg, respectively. from these results, it is inf ... | 1975 | 1234104 |
| the distribution of serotonin-containing cells in the human alimentary tract. | the distribution of serotonin-containing cells (sc cells) in the human alimentary tract was studied by means of the fluorescent histochemical method of falck et al. on surgically resected specimens. no sc cells could be observed in the nonmucosal parts of the alimentary tract and in the squamous cell epithelium of the esophagus. the richest sc cells were observed in the upper portion of the small intestine (namely the duodenum and the jejunum). the rectum was relatively rich in the distribution ... | 1975 | 1234108 |
| binding of [3h] kainic acid, and analogue of lglutamate, to brain membranes. | | 1976 | 1255165 |
| german multicentre study on the in vitro susceptibility of bacteroides species. the german bacteroides study group. | in 1990 the first german multicentre study on the in vitro susceptibility of bacteroides species was completed. employing a commercially prepared microbroth dilution assay, nine participating institutions evaluated approximately 100 consecutive isolates of bacteroides species from relevant clinical specimens. a total of 911 strains (449 bacteroides fragilis, 201 bacteroides thetaiotaomicron, 79 bacteroides ovatus, 78 bacteroides vulgatus, 77 bacteroides distasonis, 25 bacteroides uniformis, 2 ot ... | 1992 | 1295762 |
| evidence that mehp inhibits rat granulosa cell function by a protein kinase c-independent mechanism. | we have recently shown that mono-(2-ethylhexyl) phthalate (mehp), the active metabolite of the reproductive toxicant di-(ethylhexyl) phthalate (dehp), inhibited fsh- but not forskolin-, isoproterenol-, or cholera toxin-stimulated granulosa cell camp accumulation in vitro. in addition, mehp also inhibited fsh-stimulated progesterone production, a camp-dependent process. similar to mehp, the protein kinase c (pkc) activator, 12-0-tetradecanoyl-phorbol 13-acetate (tpa) has been shown to inhibit rat ... | 1992 | 1317232 |
| detubularization-induced contractile response change of the ileum following ileocystoplasty. | we reported previously that following ileocystoplasty the structure and pharmacologic response of the implanted ileum changes towards that of the bladder. specifically, the relaxation response to alpha adrenergic (methoxamine) and purinergic (atp) stimulation reverses to a contractile response one month after the ileal segment is surgically made part of the urinary bladder. the present study was designed to investigate possible signals for this change and also to determine whether bladder respon ... | 1992 | 1351953 |
| sulphasalazine fails to prevent development of mucosal ulceration and 5-lipoxygenase activity in guinea-pigs with chronic inflammatory bowel disease induced by combined bacterial immunization and oral carrageenin. | a model of inflammatory bowel disease in guinea-pigs involving a 14 day initial treatment with formalin-killed bacteroides vulgatus subcutaneously and oral carrageenin plus live b. vulgatus for 10 days was used to determine the effects of sulphasalazine 100 mg kg-1 day-1 b.i.d., p.o. given for 4, 7 and 10 days after cessation of the bacterial/carrageenin treatment on the morphological and histological states of the established disease and on the production of the principal 5-lipoxygenase product ... | 1992 | 1359081 |
| recovery of bacteroides fragilis group from clinical specimens following antimicrobial therapy. | over a period of 14 years (1973-1987), 3165 specimens submitted to the microbiology laboratory demonstrated the recovery of anaerobic bacteria. a total of 988 bacteroides fragilis group isolates were recovered (0.3 isolates per specimen). bacteroides fragilis accounted for 62% of the total of all b. fragilis group isolates, bacteroides thetaiotaomicron for 15%, bacteroides vulgatus for 8%, bacteroides ovatus for 7%, bacteroides distasonis for 6%, and bacteroides uniformis for 2%. of the 988 b. f ... | 1992 | 1393825 |
| a reassessment of the activity of the third-generation cephalosporins against anaerobes and staphylococcus aureus. | the in vitro activity of cefotaxime (ctx) alone and in combination with its metabolite desacetylcefotaxime (dctx) was evaluated against 106 isolates of bacteroides species. and 32 strains of staphylococcus aureus. all strains were recovered from infected tissues of diabetic patients. addition of dctx to the parent compound markedly increased the inhibitory activity against bacteroides species in general and bacteroides fragilis and bacteroides vulgatus in particular. no effect of the combination ... | 1992 | 1443357 |
| antibiotic sensitivity of ribosomes from wild-type and clindamycin resistant bacteroides vulgatus strains. | the sensitivity to different antibiotics of in-vitro polyuridylic acid-dependent polypeptide synthesizing system from bacteroides vulgatus ryc18f6 and two clindamycin-resistant derivatives was studied. the ribosomes from the resistant strains were not affected by concentrations of up to 0.1 mm clindamycin and lincomycin. in contrast, streptogramin b was found to cause strong stimulation of the clindamycin-resistant polymerizing systems. the modified ribosomes from the resistant strains were more ... | 1992 | 1452494 |
| pathogenicity of bacteroides fragilis group in rat intra-abdominal abscesses. | attempts were made to study the pathogenicity of some strains of bacteroides fragilis group in the rat intra-abdominal abscess model. multiple intraabdominal abscesses were produced in 50 to 70% of animals when an inoculum containing 10(9) cfu/ml of any of the five species of bacteroides fragilis group was injected. rising homologous antibody titers determined by indirect fluorescent antibody test were observed till the 3rd week when tested last, indirectly confirming the multiplication of the o ... | 1992 | 1479960 |
| mapping the biochemical trajectory of nephrotoxicity by pattern recognition of nmr urinanalysis. | | 1992 | 1489674 |
| 2,6-dichlorophenolindophenol is a competitive inhibitor for xanthine oxidase and is therefore not usable as an electron acceptor in the fluorometric assay. | xanthine oxidase has been recognized as an important source of oxygen free radicals in ischemia-reperfusion injury. in order to study this enzyme in biological tissues, the conversion of pterin (2-amino-4-hydroxypteridine) to isoxanthopterin provides the basis for a very sensitive fluorometric assay. xanthine oxidase is typically assayed in the presence of pterin only, while an electron acceptor which replaces nad+ is used to determine the combined xanthine dehydrogenase plus xanthine oxidase ac ... | 1992 | 1563644 |
| adhesion of bacteria to the cecal mucosal surface of conventional and germ-free chickens infected with eimeria tenella. | when salmonella typhimurium and clostridium perfringens were tested in conventional chickens, larger numbers of s typhimurium and c perfringens adhered to eimeria tenella-infected ceca than to uninfected ceca. in germ-free chickens, s typhimurium and c perfringens adhered to the e tenella-infected cecal mucosa more than to the uninfected cecal mucosa, but fewer bacteroides vulgatus and bifidobacterium thermophilum adhered to the e tenella-infected ceca than to the uninfected ceca. many bacteria ... | 1992 | 1575383 |
| a chronic stress state in rats: effects of repeated stress on basal corticosterone and behavior. | the chronic stress state has previously been defined as persistent visceral arousal coupled with behavioral abnormalities. to determine the number of stressor exposures necessary to induce a chronic stress state, male rats were given 2 hours of inescapable shock on 10, 7, 4, or 3 consecutive days. the 3-day stress group had the most pervasive changes in the variables measured: persistently elevated basal plasma corticosterone (cort), continued weight loss in the post-stressor period, and abnorma ... | 1992 | 1594666 |
| copulatory analgesia in male rats ensues from arousal, motor activity, and genital stimulation: blockage by manipulation and restraint. | the effect of copulation on the vocalization threshold to tail shock (vtts) was assessed in freely-moving, sexually experienced, wistar male rats. mean vtts during the first copulation was 40% above the baseline values and slightly decreased during the first postejaculatory interval (pei; 28% above baseline). vtts mean values further increased during the second copulatory series (93% above baseline and 63% during the pei). testing at the same intervals in noncopulating rats did not induce analge ... | 1992 | 1594675 |
| survey of bacteroides fragilis group susceptibility patterns in canada. | the in vitro activities of penicillin, clindamycin, chloramphenicol, metronidazole, piperacillin, piperacillin-tazobactam, ticarcillin, ticarcillin-clavulanate, ampicillin-sulbactam, cefoxitin, ceftizoxime, cefotetan, moxalactam, and imipenem against 348 bacteroides fragilis group isolates collected from six canadian cities during 1990 were determined by the national committee for clinical laboratory standards (nccls) agar dilution technique. all isolates were susceptible to chloramphenicol, met ... | 1992 | 1605600 |
| antimicrobial resistance in streptococcus pneumoniae: a south african perspective. | resistance to penicillin among south african strains of streptococcus pneumoniae increased from 4.9% in 1979 to 14.4% in 1990. except for resistance to co-trimoxazole (44%), resistance to other antimicrobial agents remained relatively low. multiply resistant strains belonged mainly to serovars 6b, 19a, 14, and, more recently, 23f. use of chloramphenicol to treat meningitis caused by strains relatively resistant to penicillin proved to be unsatisfactory, probably because of the inadequate bacteri ... | 1992 | 1617077 |
| inducible expression of ribosomal clindamycin resistance in bacteroides vulgatus. | the abilities of erythromycin and clindamycin to act as inducers of clindamycin resistance in the strain bacteroides vulgatus ryc18f6 is evaluated in vivo (efficiency of plating of inhibitory clindamycin concentrations) and in vitro [efficiency of poly(u)-directed polypeptide synthesis by ribosomes]. uninduced cells failed to grow during the first 72 h, even at a very low clindamycin concentration (0.1 microgram/ml); after induction with erythromycin or clindamycin, cells were able to form colon ... | 1992 | 1622176 |
| [the immunotherapy of herpetic stomatitis in children]. | immunoglobulin with a high titer of antiherpetic antibodies (1:640 to 1:1280) was used for the treatment of children with acute and recurrent herpetic stomatitis. the agent was injected intramuscularly, 2 to 4 injections, depending on the disease severity. the results evidence a favorable effect of the drug on the clinical and immunologic parameters of patients suffering from the acute condition and permit a conclusion that this immunoglobulin prevented the development of recurrent forms in the ... | 1991 | 1656548 |
| inflammatory bowel disease induced by combined bacterial immunization and oral carrageenan in guinea pigs. model development, histopathology, and effects of sulfasalazine. | a model of experimentally induced inflammatory bowel disease (ibd) featuring colitis, originally devised by onderdonk and co-workers in guinea pigs, was modified to establish the optimal conditions for ulcer development. upon varying the time of subcutaneous immunization with bacteroides vulgatus and concomitant oral administration of acid-degraded iota-carrageenan and viable b. vulgatus, it was found that the optimal times of administering these agents were one to two weeks and five to six days ... | 1991 | 1672517 |
| serological activity of antigens isolated from 11 bacteroides vulgatus strains. | capsular (cps) and phenol water (pw) extracts were prepared from 11 serogroup specific strains of b. vulgatus isolated from normal gut flora. the extracts were active in immunodiffusion (id) and passive hemagglutination (ha) tests. there was no correlation between sugar and protein contents and serological activity of these extracts. | 1991 | 1725095 |
| cytoarchitecture, neuronal composition, and entorhinal afferents of the flying fox hippocampus. | in a comparative approach, the anatomical organization of the hippocampus was investigated in two species of megachiropteran bats, the grey-headed flying fox, pteropus poliocephalus, and the little red flying fox, pteropus scapulatus. in general, the cytoarchitectonic appearance of the flying fox hippocampus corresponded well with that of other mammals, revealing all major subdivisions. while the dentate fascia was trilaminated with a molecular layer, a granule cell layer, and a distinct polymor ... | 1991 | 1727000 |
| the anti-tachycardic mechanism of a direct-acting vasodilator, budralazine, in rats. | the present study was undertaken to elucidate the anti-tachycardic effect of a direct-acting vasodilator, budralazine, using an electrophysiological technique. normotensive male wistar rats were used. rats were anesthetized intraperitoneally with urethane and alpha-chloralose. intravenous administration of budralazine (0.5-5.0 mg/kg) produced a dose-dependent reduction of mean arterial pressure in anesthetized rats. at doses of 0.5 and 1.0 mg/kg, budralazine induced bradycardia accompanied with ... | 1991 | 1773498 |
| [human neuroblastoma cell lines having smooth muscle cell markers]. | the neural crest gives rise to a variety of tissues, including peripheral neurons, schwann cells, melanocytes and ectomesenchymal cells, which include the smooth muscle cells of large arteries. cell lines derived from neuroblastoma (a neural crest tumor) have at least two distinct morphological cell types, a neuroblastic phenotype (n-type) and an epithelial-like phenotype (s-type) with characteristics of substrate-adhesiveness. we have analyzed 17 human neuroblastoma cell lines using a panel of ... | 1991 | 1782184 |
| measurement of alkaline phosphatase activity in serum with n-methyl-d-glucamine as a buffer: evaluation of the method for routine use. | some aspects of the measurement of alkaline phosphatase activity concentration in human serum, using n-methyl-d-glucamine as a buffer, were evaluated with a view to the possible routine use of the method. the evaluated characteristics included: the temperature-dependence of the ph of the buffer; the effect of adding the magnesium/zinc ions buffer; the effect of the serum volume fraction; the substrate-starter versus the serum-starter mode; the effect of modifying the formulation of reagents; the ... | 1991 | 1782284 |
| fibrin glue. | fibrin glue is a topical biological adhesive, the effect of which imitates the final stages of coagulation. the glue consists of a solution of concentrated human fibrinogen which is activated by the addition of bovine thrombin and calcium chloride. the resultant clot aids haemostasis and tissue sealing and is completely absorbed during wound healing without foreign body reaction or extensive fibrosis. the fibrinogen component of fibrin glue can be produced from fresh frozen plasma obtained from ... | 1991 | 1782483 |
| the effect of exposure to impulse x rays on normal and epileptic activity in rabbit brain. | exposure to x rays (20 impulses of 4 hz frequency, total dose 0.6-1.1 mgy) increased the epileptic activity of a focal area, which was produced in the visual cortex of rabbit brain by freezing with liquid nitrogen, and by stimulating with flashes of light at frequencies of 5-6 hz. the number of seizure complexes during photostimulation for 5 s increased by 80% compared with the initial level, and this effect continued for 15 min. in control animals (with no epileptic foci), a decrease was observ ... | 1991 | 1797142 |
| the effect of exposure to impulse x rays on normal and epileptic activity in rabbit brain. | exposure to x rays (20 impulses of 4 hz frequency, total dose 0.6-1.1 mgy) increased the epileptic activity of a focal area, which was produced in the visual cortex of rabbit brain by freezing with liquid nitrogen, and by stimulating with flashes of light at frequencies of 5-6 hz. the number of seizure complexes during photostimulation for 5 s increased by 80% compared with the initial level, and this effect continued for 15 min. in control animals (with no epileptic foci), a decrease was observ ... | 1991 | 1797142 |
| influence of anticoagulants on growth and vitamin k production of intestinal bacteria. | the influence of anticoagulants on growth and vitamin k production of intestinal bacteria was studied. bacteroides vulgatus and escherichia coli were grown in vitro in the presence of increasing amounts of warfarin, phenprocoumon and acenocoumarol. it was found that growth of b. vulgatus was inhibited under anaerobic conditions whereas growth of e. coli under aerobic conditions was not inhibited. a specific inhibition of vitamin k biosynthesis was not observed in either case. it is concluded tha ... | 1991 | 1798715 |
| serum and breast milk antibodies to food antigens in african mothers and relation to their diet. | results of a series of historical and recent studies of human milk and its protective antibodies against several bacterial and viral diseases and infantile diarrhea are presented. first, 1476 infants from lahore, pakistan from 3 socioeconomic groups, were observed and 300 mothers were given instruction on exclusive breast feeding. the prevalence of diarrhea indicated 60-80% protection to consistency of breast feeding, especially in summer when mothers replace breast milk with other liquids. h ... | 1991 | 1808999 |
| population of salmonella serovar typhimurium in the cecum of gnotobiotic chickens with escherichia coli and intestinal bacteria. | to test the interaction between various species of bacteria and salmonella serovar typhimurium (s. typhimurium), the population of s. typhimurium was measured in the cecum of gnotobiotic chickens in the presence of escherichia coli (e. coli) and one of the four intestinal bacteria; lactobacillus acidophilus. clostridium perfringens, bifidobacterium thermophilum and bacteroides vulgatus. competitive exclusion of s. typhimurium by di-flora chicken was not demonstrated. but the population of s. typ ... | 1991 | 1830793 |
| in vitro antibacterial activity of trospectomycin (u-63,366f) against anaerobic bacteria and aerobic gram-positive cocci in chile. | the in vitro activity of trospectomycin sulfate was compared with those of several antimicrobials, against 301 anaerobic bacteria and 613 aerobic gram-positive cocci. trospectomycin was about 4- to 32-fold more active than was spectinomycin. trospectomycin exhibited consistently good activity against all bacteroides fragilis group isolates, except bacteroides vulgatus, and against all other anaerobes comparable or higher to that of clindamycin. the trospectomycin's activity was most similar to t ... | 1991 | 1832369 |
| extracellular atp itself elicits superoxide generation in guinea pig peritoneal macrophages. | extracellular atp itself elicited the generation of superoxide (o2-) in guinea pig peritoneal macrophages associated with an increase in cytosolic calcium ([ca2+]i). the atp-induced o2- generation was completely inhibited by pretreatment with pertussis toxin (pt) accompanied by the suppression of [ca2+]i mobilization. pre-exposure to a small amount of phorbol myristate acetate (pma) primed the atp-induced generation of o2- without a change of [ca2+]i. the results suggest that atp-induced o2- gen ... | 1991 | 1851110 |
| biological functions of the water-insoluble fraction of mouse seminal vesicle fluid. i. suppression of the blastogenic response of lymphocytes. | the effects of the water-insoluble fraction of mouse seminal vesicle fluid (wif-svf) on lymphocytes was investigated to clarify its role in reproductive immunity. wif-svf inhibited the blastogenic response of t-cells to concanavalin-a (con-a), but it did not inhibit the blastogenic response of b-cells to lipopolysaccharide (lps). pretreatment of splenocytes with wif-svf did not suppress the blastogenic response of splenocytes to con-a when treated cells were washed prior to culture. wif-svf did ... | 1991 | 1906049 |
| bacterial growth control studied by flow cytometry. | by employing flow cytometry, the dna content and cell size of individual bacterial cells may be determined rapidly and with high precision. also, the number of dna replication origins in escherichia coli cells can be measured after treating the cells with rifampicin together with the cell division inhibitor cephalexin. as opposed to wild type cells, certain mutants contain, with high frequency, a number of origins different from 2n, indicating that the mutants do not initiate dna replication at ... | 1991 | 1925010 |
| the assessment of faecal flora in patients with inflammatory bowel disease by a simplified bacteriological technique. | a semi-quantitative bacteriological method was used to study faecal flora in 42 patients with crohn's disease, 37 with ulcerative colitis and 21 healthy controls. faecal homogenates were plated on primary isolation plates by a technique that allowed the growth of various microbial isolates to be assessed on a visual 1(+)-5+ score. this method was first calibrated against a standard quantitative bacteriological technique, which confirmed the reliability and reproducibility of the results obtained ... | 1991 | 1941994 |
| effect of the ltd4 receptor antagonist ly203647 on endotoxic shock sequelae in the rat. | this study investigated the effect of a novel ltd4 receptor antagonist ly203647 on salmonella enteritidis endotoxin-induced shock sequelae in anesthetized rats. ly203647 (30 mg/kg i.v.) or vehicle was given 10 min prior to endotoxin (10 mg/kg i.v.) or its vehicle, and the hematocrit, mean arterial pressure and circulating leukocyte counts were determined. ly203647 significantly inhibited endotoxin-induced hemoconcentration up to 90 min post-endotoxin (46.7 +/- 1.3 vs. 51.9 +/- 2.4% at 30 min pos ... | 1991 | 1954979 |
| efficacy and safety of recombinant human erythropoietin to prevent the anaemias of prematurity. european randomized multicenter trial. | | 1991 | 2040194 |
| pathology of mesenteric lymph nodes of buffaloes and cattle with special reference to salmonellosis. | enlarged mesenteric lymph nodes were collected from ninety buffaloes and sixty cattle slaughtered at faisalabad abattoir. among these, salmonellae were isolated from lymph nodes of 32 (21.33%) animals. maximum preponderance of salmonellosis was recorded in animals over two years of age. enlargement, pale to dark red in color, increased consistency and even calcification were the main gross pathological lesions. histopathological lesions included thickened capsule, typical lymphofollicular reacti ... | 1990 | 2134150 |
| non-immune hydrops in trisomy-18. diagnosis by vaginosonography and chorionic villus sampling in the first trimester. case report. | | 1990 | 2223690 |
| non-immune hydrops in trisomy-18. diagnosis by vaginosonography and chorionic villus sampling in the first trimester. case report. | | 1990 | 2223690 |
| application of brain microdialysis to study the pharmacology of the 5-ht1a autoreceptor. | 5-hydroxytryptamine (5-ht) receptors of the 5-ht1a subtype are localized on serotoninergic cells and dendrites in the raphe nuclei of the brain stem and are believed to regulate synaptic 5-ht release through an inhibitory influence on serotoninergic impulse flow. the effects of 5-ht1a agonists on 5-ht release can, therefore, only be detected by measurement of 5-ht release from intact serotoninergic neurones. here we review the evidence that the microdialysis technique, when applied to the anaest ... | 1990 | 2259248 |