| [intratypical antigenic differentiation of poliovirus strains circulating in mali by means of cross-adsorbed sera]. | the intratypic antigenic differentiation of poliovirus strains isolated from sick or healthy children in mali in 1975-1976 was performed. the method is based on the use of cross-adsorbed sera in the neutralization tests in which the virus is titrated by the plaque procedure or by the cytopathic effect. all the examined strains of serologic types i, ii, and iii were distinguished by the antigenic structure from the vaccine strains and were classified as "wild". quantitative differences in the ant ... | 1978 | 86237 |
| the use of trypan blue for counterstaining in the sepharose bead immunofluorescence test. the application of the test for the demonstration of primate retrovirus-specific antibodies and antigens. | the sepharose bead immunoflurorescence test was performed by counterstaining the beads with trypan blue. this results in a red staining of negative beads which allows an easy distinction from positive green-fluorescent beads. sepharose beads conjugated with viral proteins or antiviral antibodies were used to demonstrate mason-pfizer monkey virus (mpmw)- and simian sarcoma virus (ssv) - specific antigens or antibodies. the test shows a high sensitivity and specificity and needs a small amount of ... | 1978 | 86253 |
| inheritance of tolerance susceptibility to human gamma-globulin in congenic mice. | the genetic control of susceptibility to tolerance induction with human gamma-globulin (hgg) was studied by using h-2 congenic mice. strains tested that were congenic with c57bl/10sn were completely tolerized by 1.0 mg deaggregated hgg. in contrast a/sn mice showed full tolerance whereas a.sw mice were only intermediately tolerant. it was further shown that (b10 x sjl)f1 mice could be rendered tolerant but (b10.s x sjl)f1 mice could not. these data indicate a role for h-2 linked genes in control ... | 1979 | 89162 |
| acid fast staining of oxidized neuromelanin and lipofuscin in the human brain. | acid fast staining of the bleached residuum of substantia nigra neuromelanin and of oxidized inferior olive lipofuscin was demonstrated in paraffin and frozen sections stained with the acetic acid, carbol fuchsin method of barbeito-lopez and the aldehyde fuchsin method of gomori. acid fast staining occurred when sections were exposed to a prestain oxidation with potassium permanganate in conjunction with a poststain differentiation with dilute hydrochloric acid. the acid fast staining with aceti ... | 1979 | 89189 |
| histamine-releasing effect of a corticotrophin derivative. ii. mechanism of action of histamine release by c 44 680-ba, compared with that of cpd. 48/80, dextran and triton. | the mechanism of the histamine-liberating action of the synthetic polypeptide c 44 680-ba, an alkyl-prolyl derivative of beta 1-19 corticotrophin, was investigated and compared with those of compound 48/80, dextran, melittin and triton x-100. it was found that the release of histamine from rat peritoneal cells induced by the polypeptide is dependent on temperature, ph, calcium ions and energy-providing processes. in regard to these criteria, the mode of action of this histamine liberator resembl ... | 1979 | 89804 |
| distribution of myosin isoenzymes among skeletal muscle fiber types. | using an immunocytochemical approach, we have demonstrated a preferential distribution of myosin isoenzymes with respect to the pattern of fiber types in skeletal muscles of the rat. in an earlier study, we had shown that fluorescein-labeled antibody against "white" myosin from the chicken pectoralis stained all the white, intermediate and about half the red fibers of the rat diaphragm, a fast-twitch muscle (gauthier and lowey, 1977). we have now extended this study to include antibodies prepare ... | 1979 | 90047 |
| the use of avidin-biotin interaction in immunoenzymatic techniques. | biotin was covalently attached to antibodies, antigens and enzymes, and the effects of this labeling on the antigen and antibody binding capacity and on enzymatic activity were tested. based on avidin-biotin interaction, the labeled proteins were used in quantitative enzyme-immunoassay and enzyme-immunohistochemical staining procedures. two procedures were developed. in the first procedure, named the bridged avidin-biotin (brab) technique four steps were used sequentially in order to quantify or ... | 1979 | 90074 |
| the expression of a human b-lymphocyte antigen and surface membrane immunoglobulin by lymphoid cells from patients with lymphocytic lymphoma, multiple myeloma and benign monoclonal gammapathy. | | 1979 | 90449 |
| the intra-nucleolar localization of amplified rdna in xenopus laevis oocytes. | a fluorescent feulgen-stain was adapted in order to demonstrate dna-containing structures inside the amplified nucleoli of xenopus laevis. at all stages of oogenesis this method reveals granules or complex structures of dna in each nucleolus. the micronucleoli which do not stain with this method and which do not reveal an internal structure in low molarity saline, unlike real nucleoli are considered as nucleolus-like bodies. the dna-containing structures in the nucleoli can be composed of one or ... | 1979 | 90588 |
| the indirect rat mast cell degranulation test reconsidered. | the indirect rat mast cell degranulation test for the detection of human reaginic antibodies was reinvestigated. when the mast cells were incubated with human reaginic serum at concentrations exceeding approximately 3%, a massive, non-specific histamine release occurred, irrespective of the presence or absence of allergen. at tolerated serum concentrations (below 3%), the allergen concentration did not influence the release process. we conclude that the indirect rat mast cell test is not applica ... | 1979 | 90655 |
| antigens in penicillin allergy. iv. induction of ige antibody response in mice after daily treatment with contaminated but not with pure penicillin. | | 1979 | 90657 |
| characterization of a type-c virus produced by co-cultures of human leukemic bone-marrow and fetal canine thymus cells. | the putative human helper virus ska-21/a204v, isolated by nooter et al. in 1977 from human leukemic bone-marrow cells following co-culture with normal fetal canine thymus cells, cf2th, has been characterized with respect to its major viral core protein, reverse transcriptase, and nucleic acid sequences. the results of these analyses show that this virus is not distinguishable from the woolly monkey type-c virus, ssav-1, by the techniques employed. | 1979 | 90662 |
| trace polypeptides in cellular extracts and human body fluids detected by two-dimensional electrophoresis and a highly sensitive silver stain. | development of a highly sensitive silver stain permits the characterization of trace cellular and body fluid proteins separated by the two-dimensional electrophoresis technique of o'farrell. many of the proteins detected by the silver stain in urine, spinal fluid, amniotic fluid, and cells were undetected with the widely used coomassie blue stain. trace polypeptides observed in escherichia coli cell lysates with this silver stain could be detected previously only by growth in radioactive precurs ... | 1979 | 92027 |
| epithelioid sarcoma: ultrastructural observation of lymphoid cell-induced lysis of tumor cells. | epithelioid sarcoma of the palm of 7 months' duration was observed in a 30-year-old man. six months after wide surgical excision there was no evidence of recurrence or metastasis. by light microscopic examination the tumor showed typical nodular arrangement of malignant cells, with necrosis of these cells in the centers of the nodules. patchy lymphocytic infiltrates were observed at the peripheries of the nodules and also extended in places between the tumor cells. other types of inflammatory ce ... | 1979 | 92147 |
| membrane-bound mitochondrial dna: isolation, transcription and protein composition. | mitochondrial membrane-bound dna complex from bovine heart mitochondria lysed in the presence of triton x-100 was isolated by differential centrifugation. the yield of "nucleoid" is about 30 microgram protein/mg mitochondrial protein. it contains about 3-5 microgram dna/mg protein and varying amounts of rna. the heart mitochondrial nucleoid actively synthesizes rna. the nucleoid fraction contains about sixteen different proteins as evidenced by urea-sds gel electrophoresis and about twenty-one p ... | 1979 | 92288 |
| the effect of dose loading and of double labelling with 57co and 125i on the tissue distribution in animals. | dose loading effects upon the performance of 57co-bleomycin as a tumor localizing agent have been investigated in rhabdomyosarcoma bearing wag/ry rats. the addition of non-radioactively labelled co-bleomycin increased the relative uptake of 57co-bleomycin in rapid growing tumors, but the addition of non-chelated bleomycin had no influence at all. in our experimental system, iodinated bleomycin generally labelled by reaction with icl, was found to be an unsatisfactory tumor localizing agent. in o ... | 1979 | 93048 |
| [studies on moraxella. i. a new selective medium for moraxella sp. (author's transl)]. | | 1977 | 101603 |
| [volatile fatty acids in gastrointestinal diseases in young infants]. | | 1978 | 101958 |
| purification and some properties of porcine brain mitochondrial monoamine oxidase b. | | 1978 | 103556 |
| [isolation of treponemas from the colon of pigs with clinical dysentery]. | optimal culture conditions in artificial nutritive media were determined for a defined avirulent strain of treponema hyodysenteriae and for four field strains of treponemas in pigs with clinical dysentery. the treponemas were isolated with the use of milliporous filters with pores of 0.3 micrometer in diameter, which were located on the surface of blood agar. no significant difference in the influence of equine, bovine or sheep blood on the growth of treponemas was determined. the commercial amo ... | 1979 | 105448 |
| affinity chromatography of endoglucanase of trichoderma viride by concanavalin a-agarose. | endoglucanase (c kappa cellulase) and cellobiase are often cross-contaminated in separation procedures by ion-exchange chromatography such as deae-cellulose. by using concanavalian a (con a)-agarose chromatography, c kappa cellulase and cellobiase from trichoderma virde can be separated. c kappa cellulase showed affinity toward con a. indicating a glycoprotein containing alpha-d-mannopyransyl and alpha-d-glucopyranosyl end groups or internal 2-o-d-mannopyranosyl residues in sugar moieties. this ... | 1979 | 106904 |
| [homologous transplants in the middle ear (author's transl)]. | the original concept of tympanoplasty included besides the closure of the middle ear, the removal of pathological middle ear components leaving intact the functional and healthy components. the special conditions that prevail in the middle ear (aeration, absence of intrinsic trauma) allow the reconstruction of the middle ear mechanics to a great extent. animal experiments and clinical experience reveal good transplantation properties of denatured and preserved ear ossicles. they are partly repla ... | 1975 | 123985 |
| [pseudomonas aeruginosa--the causative agent of infection in birds]. | pseudomonas aeruginosa was isolated from the viscera of diseased and dead birds (young and adult chickens) as well as from 9 poultry houses on seven farms. its typing was performed on the basis of the blue-green coloration of the cultures, their hemolytic activity, the pleasant odour of lime-tree, the growth in culturing at 42 degrees c, the presence of the pyocianin pigment, and the positive results of the cytochromoxidase test after gaby and hadley and the biochemical test after kovacs. the pa ... | 1975 | 127412 |
| steroid production by quartered and capsular rat adrenal gland in response to haemorrhage. | 60 min after rapid bleeding (1.5--2.0 per cent of b. w.) both aldosterone and corticosterone production rate by quartered rat adrenals were found to be elevated. however, no difference was observed in the rate of aldosterone and corticosterone production by capsular adrenals of sham operated and hypovolaemic rats. corticosterone production rate by decapsulated adrenals was much more higher after haemorrhage than in the control group. the same alterations could be observed incubating adrenal tiss ... | 1975 | 179800 |
| radioimmunoassay of triiodothyronine in human serum with special reference to methodological problems. | a radioimmunoassay for measuring the level of triiodothyronine in human serum has been developed and studied with respect to interference from hormone-binding substances present in human serum. dextran-coated charcoal was used for the separation of free and antibody-bound hormone. ans (8-anilino-1-naphthalenesulfonic acid) was used to reduce the interference from hormone-binding substances in serum. extraction of the hormone by ethanol before the radioimmunoassay did not solve the problem of int ... | 1976 | 181837 |
| effectiveness of orally administered bordetella pertussis vaccine in mice. | oral administration of killed bordetella pertussis organisms to mice results in increased resistance to an intracerebral infection with virulent b. pertussis cells. the rate of survival is dependent on the dose of antigen. but besides specific systemic immunity, which is persistent over a long period, also transient non-specific resistance is increased. these effects are evidently induced without penetration of bacterial substances into the circulation. | 1976 | 182602 |
| [radiolabeling of surface sialyl and galactosyl residues of zajdela's ascitic hepatoma cells]. | | 1978 | 208658 |
| a direct radioimmunoassay for tonin. | a sensitive radioimmunoassay for the measurement of tonin is described. it is based on the use of antibodies produced in rabbits against highly purified tonin obtained from rat submaxillary gland. a radioiodinated enzyme with high specific activity was obtained by the chloramine-t method. optimal conditions for radioimmunoprecipitation were established and the double-antibody method was used to separate bound and free tonin. the radioimmunoassay may be used to measure tonin in amounts as low as ... | 1978 | 210909 |
| presence of viral particles in the salivary gland of calomys musculinus infected with junin virus by a natural route. | calomys musculinus, a wild cricetid rodent, is one of the main reservoirs of junin virus. six of these animals were infected by being placed in close contact with animals that had been experimentally infected with the virus. they were sacrificed at 10, 15 and 20 months after contact, and their salivary glands were studied by ultrastructural, immunohistochemical and virological methods. two animals developed chronic viremia and low titers of complement-fixing antibodies. these animals were the on ... | 1979 | 218895 |
| fecal bacteria in south african rural blacks and other population groups. | quantitative studies were performed on fecal flora of three population groups consuming different diets. twenty rural black south africans and 22 japanese, representing groups at low risk for carcinoma of the colon, were compared with 41 north americans from a high-risk population. specimens taken immediately after defecation were mixed and processed under anaerobic conditions. after the initial incubation, roll tubes were shipped to the united states for final identification. bacterioides and b ... | 1979 | 447500 |
| synthesis of alpha-ketoglutarate by reductive carboxylation of succinate in veillonella, selenomonas, and bacteriodes species. | evidence for reductive carboxylation of succinate to synthesize alpha-ketoglutarate was sought in anaerobic heterotrophs from the rumen and from other anaerobic habitats. cultures were grown in media containing unlabeled energy substrates plus [14c]succinate, and synthesis of cellular glutamate with a much higher specific activity than that of cellular asparate was taken as evidence for alpha-ketoglutarate synthase activity. our results indicate alpha-ketoglutarate synthase functions in selenomo ... | 1979 | 533772 |
| factors affecting production of catalase by bacteroides. | several variables affected the production of catalase by members of the "bacteroides fragilis group" of anaerobic bacteria. both media yielded higher catalase levels than the respective agar media. addition of hemin to media after autoclave sterilization, rather than before, significantly increased production of catalase. both of these variables could be related to the available hemin concentration present in the medium being tested. significantly higher amounts of hemin were required for catal ... | 1978 | 730827 |
| new approach to caloric stimulation of the vestibular receptor. | a new caloric testing method is described. during continuous aural irrigation, fluid is switched between hot and cold values at times computed according to a mathematical model of heat conduction in the labyrinth area. as a result, the induced temperature difference across the lateral semicircular canal describes an approximately sinusoidal time course, reaching peak values of equal magnitude but opposite sign. the magnitude of the caloric stimulus may be selected by choosing appropriate irrigat ... | 1975 | 1081359 |
| [effect of an oxygen-vitamin complex on general and immunological reactivity of irradiated animals]. | | 1975 | 1153721 |
| [studies on the use of wheat rich in crude proteins in the feeding of broilers. 2. carcass yields and carcass composition]. | studies were made to investigate whether the the substitution of maize for high-protein wheat fed in combination with different protein in fattening rations to broilers would influence the carcass yields and chemical carcass composition of broilers. the described dietary regime was not found to have a significant influence on the different criteria of carcass composition (roaster carcass, flesh, abdominal fat, total proportion of utilizable parts) with the exception of the relative proportion of ... | 1975 | 1233971 |
| german multicentre study on the in vitro susceptibility of bacteroides species. the german bacteroides study group. | in 1990 the first german multicentre study on the in vitro susceptibility of bacteroides species was completed. employing a commercially prepared microbroth dilution assay, nine participating institutions evaluated approximately 100 consecutive isolates of bacteroides species from relevant clinical specimens. a total of 911 strains (449 bacteroides fragilis, 201 bacteroides thetaiotaomicron, 79 bacteroides ovatus, 78 bacteroides vulgatus, 77 bacteroides distasonis, 25 bacteroides uniformis, 2 ot ... | 1992 | 1295762 |
| effect of clavulanic acid, sulbactam and tazobactam on three different beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum. | the effect of three beta-lactamase inhibitors, clavulanic acid, sulbactam and tazobactam used in clinical practice were compared for their activity against purified beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum. the enzymes from b. uniformis and c. butyricum were produced in fermenters under controlled growth conditions and the enzyme from f. nucleatum was produced in batch cultures. purification of the beta-lactamases was achieved by anion-exchang ... | 1992 | 1331018 |
| effect of beta-lactamase inhibitors on beta-lactamases from anaerobic bacteria. | three beta-lactamase inhibitors in clinical use--clavulanic acid, sulbactam and tazobactam--were investigated for their activity on beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum. purification of the beta-lactamases was carried out by anion-exchange chromatography, gel filtration and fplc. the inactivation of beta-lactamase activity was determined spectrophotometrically with nitrocefin as substrate. various concentrations of the inhibitors were prei ... | 1992 | 1338315 |
| recovery of bacteroides fragilis group from clinical specimens following antimicrobial therapy. | over a period of 14 years (1973-1987), 3165 specimens submitted to the microbiology laboratory demonstrated the recovery of anaerobic bacteria. a total of 988 bacteroides fragilis group isolates were recovered (0.3 isolates per specimen). bacteroides fragilis accounted for 62% of the total of all b. fragilis group isolates, bacteroides thetaiotaomicron for 15%, bacteroides vulgatus for 8%, bacteroides ovatus for 7%, bacteroides distasonis for 6%, and bacteroides uniformis for 2%. of the 988 b. f ... | 1992 | 1393825 |
| antibiotic sensitivity of the bacteroides fragilis group in europe. european study group. | rates of resistance to 12 antibiotics were determined for 1,289 isolates of bacteroides fragilis group submitted in 1988-1989 by 22 laboratories in 15 european countries. there was no resistance to metronidazole (breakpoint 8 mg/l) and only one isolate was resistant to chloramphenicol (breakpoint 8 mg/l). resistance was uncommon for imipenem (0.3% at greater than 4 mg/l), amoxicillin/clavulanate (1% at greater than 8 mg/l), cefoxitin (3% at greater than 32 mg/l), mezlocillin (6% at greater than ... | 1992 | 1396748 |
| thermally-induced delayed fluorescence of photosystem i and ii chlorophyll in thermophilic cyanobacterium synechococcus elongatus. | stationary delayed fluorescence (df) of chlorophyll in isolated membrane preparations from thermophilic cyanobacterium synechococcus elongatus was investigated as a function of temperature. two peaks at different temperatures were observed. the low-temperature peak (54-60 degrees c) coincided with the main maximum of the thermally-induced delayed fluorescence of chlorophyll in intact cells and psii-particles with active oxygen-evolving system. the high-temperature peak (78 degrees c) coincided w ... | 1992 | 1426973 |
| evidence for natural transfer of a tetracycline resistance gene between bacteria from the human colon and bacteria from the bovine rumen. | previously, we demonstrated conjugal transfer of a specially constructed shuttle vector, prdb5, from the human colonic anaerobe bacteroides uniformis to the ruminal anaerobe prevotella (bacteroides) ruminicola b(1)4. we have now shown that naturally occurring gene transfer elements in bacteroides species and prevotella ruminicola can also be transferred between these two genera. a self-transmissible chromosomal element originally found in a clinical isolate of bacteroides fragilis (tcr emr 12256 ... | 1992 | 1599250 |
| conjugal transfer of a shuttle vector from the human colonic anaerobe bacteroides uniformis to the ruminal anaerobe prevotella (bacteroides) ruminicola b(1)4. | prevotella ruminicola (formerly bacteroides ruminicola) is an anaerobic, gram-negative, polysaccharide-degrading bacterium which is found in the rumina of cattle. since p. ruminicola is thought to make an important contribution to digestion of plant material in rumina, the ability to alter this strain genetically might help improve the efficiency of rumen fermentation. however, previously there has been no way to introduce foreign dna into p. ruminicola strains. in this study we transferred a sh ... | 1991 | 1768083 |
| the anti-tachycardic mechanism of a direct-acting vasodilator, budralazine, in rats. | the present study was undertaken to elucidate the anti-tachycardic effect of a direct-acting vasodilator, budralazine, using an electrophysiological technique. normotensive male wistar rats were used. rats were anesthetized intraperitoneally with urethane and alpha-chloralose. intravenous administration of budralazine (0.5-5.0 mg/kg) produced a dose-dependent reduction of mean arterial pressure in anesthetized rats. at doses of 0.5 and 1.0 mg/kg, budralazine induced bradycardia accompanied with ... | 1991 | 1773498 |
| effect of testosterone on the pressor response to common carotid occlusion in gonadectomized conscious male rats with lesion of the median eminence. | the influence of testosterone on the development of the pressor response to common carotid occlusion was investigated in control and median eminence-lesioned male rats. in control rats (n = 9), gonadectomy performed 21 days before the experiments reduced by 22% (from 51 +/- 2 to 40 +/- 2 mmhg) and treatment with testosterone (300 micrograms for 4 days before the measurements) increased the initial peak pressor response (from 51 +/- 2 to 57 +/- 2 mmhg) which depends on carotid innervation. the ma ... | 1991 | 1797293 |
| effect of testosterone on the pressor response to common carotid occlusion in gonadectomized conscious male rats with lesion of the median eminence. | the influence of testosterone on the development of the pressor response to common carotid occlusion was investigated in control and median eminence-lesioned male rats. in control rats (n = 9), gonadectomy performed 21 days before the experiments reduced by 22% (from 51 +/- 2 to 40 +/- 2 mmhg) and treatment with testosterone (300 micrograms for 4 days before the measurements) increased the initial peak pressor response (from 51 +/- 2 to 57 +/- 2 mmhg) which depends on carotid innervation. the ma ... | 1991 | 1797293 |
| evaluation of the rapid id 32a system for the identification of bacteroides fragilis and related organisms. | this study evaluated the ability of a rapid identification system for anaerobic bacteria, atb 32a, now renamed rapid id 32a (api-biomérieux uk ltd., basingstoke), to identify accurately 74 strains of the 'b. fragilis group'. atb 32a identified correctly 78.4% of strains to species level, without supplemental tests. the percentage of strains identified to species level rose to 94.6% when a supplementary test (advised by biomérieux) for catalase production was used to differentiate between bactero ... | 1991 | 1960112 |
| the role of alpha- and beta-adrenoceptor subtypes in mediating the effects of catecholamines on fasting glucose and insulin concentrations in the rat. | 1. the role of alpha- and beta-adrenoceptor subtypes in the regulation of plasma glucose and immunoreactive insulin (iri) levels has been investigated in normal conscious fasted rats by employing selective agonists and antagonists. 2. adrenaline (0.2 mg kg-1)-induced hyperglycaemia was abolished by the selective alpha 2-adrenoceptor antagonist idazoxan (1.0 mg kg-1), unaltered by non-selective beta-adrenoceptor blockade (propranolol, 1.0 mg kg-1) and potentiated by the selective alpha 1-adrenoce ... | 1990 | 1976400 |
| plasmid transformation of bacteroides spp. by electroporation. | transformation of bacteroides spp. with a variety of plasmid dnas was accomplished using electroporation. the standard transformation assay system used to deduce the optimal electroporation parameters employed a 50-to 100-fold concentrated cell suspension of mid-logarithmic phase bacteroides fragilis strain 638 and the 5.4-kb clindamycin resistance (ccr) vector, pbi191. a variety of electroporation buffers were used successfully in transformation experiments but of these, 1 mm mgcl2 in 10% glyce ... | 1990 | 2096397 |
| role of bacteroides uniformis in susceptibility of ta:cf# 1 mice to infection by treponema hyodysenteriae. | among the various strains and colonies of mice tested specific-pathogen-free (spf) ta:cf#1 mice exhibited the highest susceptibility to treponema hyodysenteriae infection. staphylococcus epidermidis 1047, streptococcus faecalis 2039, lactobacillus murinus 3010, escherichia coli 1049, and bacteroides thetaiotaomicron 3008 were administered to the ta:cf#1 mice. b. uniformis ik, which appeared to colonize in the breeding facility under spf conditions, was isolated from the cecal content of a ta:cf# ... | 1990 | 2148089 |
| a cryptic 65-kilobase-pair transposonlike element isolated from bacteroides uniformis has homology with bacteroides conjugal tetracycline resistance elements. | a 65-kilobase-pair element, xbu4422, which has some transposonlike characteristics but carries no known antibiotic resistance genes, has been isolated from bacteroides uniformis 0061. xbu4422 was trapped on bacteroides-escherichia coli shuttle vectors during experiments in which one of the conjugal bacteroides tetracycline resistance (tcr) elements was being used to mobilize the shuttle vectors to bacteroides recipients. results of southern hybridization experiments showed that xbu4422 is normal ... | 1990 | 2156799 |
| the region of a bacteroides conjugal chromosomal tetracycline resistance element which is responsible for production of plasmidlike forms from unlinked chromosomal dna might also be involved in transfer of the element. | large (greater than 50 kilobases) conjugal chromosomal tetracycline resistance (tcr) elements have been found in many human colonic bacteroides strains. recently, n. b. shoemaker and a. a. salyers (j. bacteriol, 170:1651-1657, 1988) reported that some of these tcr elements appeared to mediate production of plasmidlike forms, nbu1 and nbu2, from an unlinked region of the chromosome of bacteroides uniformis 0061. production of the plasmidlike forms and the transfer frequency of the tcr elements we ... | 1990 | 2165473 |
| [new antitubercular drugs]. | | 1990 | 2168041 |
| non-immune hydrops in trisomy-18. diagnosis by vaginosonography and chorionic villus sampling in the first trimester. case report. | | 1990 | 2223690 |
| heterologous expression of the bacteroides ruminicola xylanase gene in bacteroides fragilis and bacteroides uniformis. | a cloned xylanase gene from the ruminal bacterium bacteroides ruminicola 23 was transferred by conjugation into the colonic species bacteroides fragilis and bacteroides uniformis by using the escherichia coli-bacteroides shuttle vector pval-1. the cloned gene was expressed in both species, and xylanase specific activity in crude extracts was found to be at least 1400-fold greater than that found in the b. ruminicola strain. analysis of crude extract proteins from the recombinant b. fragilis by s ... | 1990 | 2323546 |
| effects of serum testosterone level with buserelin on the activities of drug and testosterone hydroxylase and on the content of a male-specific form of cytochrome p-450 in male rats. | the effects of administration of buserelin, a synthetic agonist of lutenizing hormone-releasing hormone (lh-rh), on the content of p-450-male, a male specific form of cytochrome p-450, and the activities of drug and testosterone hydroxylases were examined in liver microsomes of male rats. administration of buserelin resulted in a decrease in the serum level of testosterone in a dose-dependent manner. similar decreases were seen in the activities of aminopyrine n-demethylase, 7-propoxycoumarin o- ... | 1989 | 2492807 |
| morphological response of mycoplasma to fleroxacin. | quinolones exhibit partially reduced activity in vitro against genital mycoplasma. therefore, the morphological response to fleroxacin, a difluorinated quinolone, by mycoplasma hominis was studied. even at subinhibitory concentrations the ultrastructural investigations revealed distinct alterations such as cytolysis, vacuole and cell "ghost" formation. the typical dumbbell-shaped cells observed with monofluorinated compounds were not found, which may indicate a qualitatively different mode of ac ... | 1989 | 2515157 |
| evidence that sindbis virus nsp2 is an autoprotease which processes the virus nonstructural polyprotein. | the four nonstructural proteins (nsp1-4) of sindbis virus, a member of the togaviridae family, are initially expressed from the 5' segment of the single-stranded genomic (+)rna as a polyprotein which is subsequently proteolytically processed. in attempts to identify the protease acting on this nonstructural polyprotein, we established a coupled in polyprotein, we established a coupled in vitro transcription-translation system which was able to faithfully process the major polyprotein when an mrn ... | 1989 | 2525839 |
| specificity spillover at the hormone receptor--exploring its role in human disease. | | 1989 | 2537464 |
| electroporation induced transformation of bacteroides ruminicola and bacteroides uniformis by plasmid dna. | frequencies of greater than 10(5) transformants per microgram dna were achieved in bacteroides ruminicola f101 by electroporation of cells under anaerobic conditions, using the 19.5 kbp tetracycline resistance plasmid prri4. similar procedures gave frequences of 10(6) erythromycin resistant transformants per microgram dna with the shuttle plasmid pdp1 (19 kbp) in bacteroides uniformis. transformation of b. uniformis occurred at a far lower frequency (10(3) micrograms) when pdp1 dna was derived f ... | 1989 | 2599352 |
| omental autoimplantation of splenic tissue and intravenous pneumococcal challenge. a comparative study in rats. | splenectomized subjects show a higher incidence of myocardial ischemia and of overwhelming post-splenectomy infection (opsi). it is doubtful that implanted splenic tissue guarantees an adequate protection from opsi. the histological characteristics and the capacity of protection from opsi of two models of omental autoimplantation of splenic tissue in rats were examined. the implanted splenic tissue offers a significant protection from pneumococcal sepsis, even though there is no relation between ... | 1989 | 2628385 |
| cloning and characterization of a bacteroides conjugal tetracycline-erythromycin resistance element by using a shuttle cosmid vector. | the bacteroides conjugal tetracycline resistance (tcr) elements appear not to be plasmids. in many cases, resistance to erythromycin (emr) is cotransferred with tcr. using a newly constructed shuttle cosmid, pnjr1, we cloned 44 to 50 kilobase pairs of a conjugal tcr emr element on overlapping cosmid clones. cosmid libraries were made in escherichia coli with dna from the original clinical bacteroides thetaiotaomicron dot strain containing tcr emr-dot or from a bacteroides uniformis tcr emr-dot t ... | 1989 | 2646276 |
| monoclonal antibody-defined human endothelial antigens as vascular markers. | a review is given of human endothelial antigens recognized by monoclonal antibodies and used as vascular markers. these antigens can be classified tentatively into two categories that partly overlap: 1) differentiation markers and 2) antigens involved in specific cellular functions. monoclonal antibodies recognizing endothelial differentiation markers reacting with all types of human endothelium can be regarded as constitutive endothelial markers. other differentiation markers have a restricted ... | 1989 | 2666520 |
| pathogenicity of the bacteroides fragilis group. | the bacteroides fragilis group is one of the most important pathogens in polymicrobial infections. the distribution of the different members of the b. fragilis group in clinical infections varies. bacteroides fragilis accounts for 63 percent of all the group isolates, bacteroides thetaiotaomicron for 14 percent, bacteroides vulgatus and bacteroides ovatus for seven percent each, bacteroides distasonis for six percent and bacteroides uniformis for two percent. all members of the group induced bac ... | 1989 | 2679351 |
| minitek urea disk test, a sensitive and cost-effective method to screen for campylobacter pylori in gastric biopsies. | one hundred fifty-five biopsy specimens from the gastric mucosa of 81 patients undergoing routine endoscopy procedures were tested for the presence of campylobacter pylori by three methods: gram stain, culture, and modified minitek, a rapid urea disk test (bbl microbiology systems, cockeysville, md.). twenty-nine patients were infected with c. pylori. sensitivities and specificities of detection were 100 and 94% with the minitek test and 93 and 100% with gram stain, respectively. rapid testing b ... | 1989 | 2687317 |
| design, expression, and crystallization of recombinant lectin from the garden pea (pisum sativum). | the propeptide form of the lectin from the garden pea (pisum sativum agglutinin) has been expressed in escherichia coli by attaching its cdna to an inducible promoter. by a number of criteria, including the ability to form dimers, hemagglutination titer, western blot, and enzyme-linked immunosorbent assay, the resulting propeptide molecule is virtually indistinguishable from the mature proteolytically processed lectin isolated from peas. preliminary crystallization experiments using the recombin ... | 1989 | 2708344 |
| protein kinase c is activated in glomeruli from streptozotocin diabetic rats. possible mediation by glucose. | glomerular inositol content and the turnover of polyphosphoinositides was reduced by 58% in 1-2 wk streptozotocin diabetic rats. addition of inositol to the incubation medium increased polyphosphoinositide turnover in glomeruli from diabetic rats to control values. despite the reduction in inositol content and polyphosphoinositide turnover, protein kinase c was activated in glomeruli from diabetic rats, as assessed by an increase in the percentage of enzyme activity associated with the particula ... | 1989 | 2708528 |
| use of the glucose oxidase/peroxidase method for glucose assay leads to overestimation of the inhibition of gluconeogenesis by aminopyrine. | 4-aminoantipyrine strongly inhibits glucose determination by the glucose oxidase/peroxidase/dianisidine assay but does not interfere in the assay using glucose-6-phosphate dehydrogenase, hexokinase and atp. as a result, the inhibition of gluconeogenesis by aminopyrine reported to be 50-90% (bánhegyi, g., mandl, j., antoni, f. and garzó, t. (1987) biochim. biophys. acta 927, 406-416) is strongly overestimated and amounts to only 10-30%. | 1989 | 2713417 |
| antibody responses and protection in mice immunized with herpes simplex virus type 1 antigen immune-stimulating complex preparations. | the formation of immune-stimulating complexes (iscoms) obtained by mixing the glycoside quil a with an antigen preparation derived from herpes simplex virus type 1 (hsv-1)-infected cell cultures using a zwitterionic detergent is described. the hsv-1 antigen preparation incorporated into iscoms elicited significantly greater antibody responses in mice than the same preparation administered together with aluminium hydroxide gel, and provided complete protection against hsv-1 or hsv-2 lethal, syste ... | 1989 | 2769234 |
| adverse effects of the use of unusual phenethylamine compounds sold as illicit amphetamine. | a 40-year-old man, who had regularly taken illicit amphetamine by intra-nasal inhalation for several years without ill effects, was admitted to hospital with signs of massive adrenergic overstimulation shortly after inhaling material which he had purchased in the belief that it was amphetamine. the administration of the beta-blocker practolol produced a paradoxical increase in blood pressure. after his discharge from hospital he suffered disabling feelings of anxiety for several weeks. analysis ... | 1989 | 2770471 |
| direct measurement of basolateral membrane potentials from cells of the macula densa. | at the present time, little is known concerning the electrophysiology of the cells of the macula densa and whether or not these cells are electrically responsive to alterations in luminal fluid composition. to investigate this issue, cortical thick ascending limbs (ctal) containing macula densa and attached glomeruli were dissected from rabbit kidney and the ctal perfused in vitro. basolateral membrane potential (vbl) was measured with microelectrodes in macula densa cells and, for comparison, i ... | 1989 | 2782426 |
| the role of calcium in the development of diastolic force during hypoxia in rat myocardium. | | 1989 | 2798517 |
| spontaneous tumors in f-344/jcl rats. | the age-related incidence of spontaneously occurring neoplastic and non-neoplastic lesions in untreated f-344/jcl rats, used as controls in carcinogenicity testing, were studied from the histological examination of tissues from 469 males and 354 females. the incidence of spontaneous tumors was 83.2% in the males and 71.2% in the females. the most common neoplasms were leukemia (males: 24.3%, females: 24.0%), pituitary adenoma (males: 16.0%, females: 45.2%), pheochromocytoma (males: 14.7%, female ... | 1989 | 2810400 |
| tetracycline-dependent appearance of plasmidlike forms in bacteroides uniformis 0061 mediated by conjugal bacteroides tetracycline resistance elements. | some human colonic bacteroides strains carry conjugal tetracycline resistance (tcr) elements, which are thought to be chromosomal. we have found that some of these tcr elements can mediate the appearance of plasmidlike forms in bacteroides uniformis 0061. when b. uniformis 0061, containing a conjugal tcr element designated tcr erl, was grown in medium containing tetracycline (1 microgram/ml), two circular dna forms were found in the alkaline plasmid preparations: nbu1 (10.3 +/- 0.5 kilobases) an ... | 1988 | 2832373 |
| high-performance liquid chromatographic separation and immunological characterization of soluble bovine viral diarrhea virus antigen. | sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blot analysis of the protein extracts from bovine viral diarrhea virus (bvdv, singer strain) infected primary calf testicle cells (soluble antigen) showed the presence of four virus specific polypeptides of 105, 90, 84 and 67 kilodaltons (kd) the 84-kd being the most abundant. anion-exchange high-performance liquid chromatography (hplc) of soluble antigen separated the virus specific polypeptides in individual peaks while the ... | 1988 | 2832429 |
| [human chorionic prealbumin: its identification, physicochemical properties and detection in the blood serum in trophoblastic disease]. | human chorionic prealbumin (hcpa) was immunochemically identified and isolated in pure form from immature placenta, particularly, from chorion. comparative immunodiffusion analysis shows that hcpa differs from the known placenta-specific proteins. hcpa is a glycoprotein with mw about 100 kd determined by gel-filtration and 45 kd by sds polyacrylamide gel electrophoresis. by immunodiffusion hcpa was not found in fetal and adult tissues and in biological fluids. hcpa was discovered in patients wit ... | 1988 | 2848598 |
| antiviral chemoprophylaxis for cytomegalovirus infection. | | 1988 | 2849602 |
| low-grade lymphomas in young homosexual men without lav/htlv iii infection. | | 1986 | 2877052 |
| [adaptive effect and withdrawal syndrome of propranolol in relation to norepinephrine and epinephrine]. | | 1985 | 2934953 |
| structure and expression of the hepatitis b virus genome. | | 1987 | 2948897 |
| [interferon production in vitro]. | interferon production in vitro was induced by poly i: c and pha in leukocytes obtained from patients with cancer, benign diseases and control subjects. the interferon response per lymphocyte was relatively constant in all groups. however, interferon production was slightly inhibited in patients receiving cancer chemotherapy. the peak response of interferon occurred at 48 hours after the initiation of the combined culture in each specimen. from our observations, it was suggested that interferon p ... | 1987 | 2952067 |
| insulin action inhibits insulin-like growth factor-ii (igf-ii) receptor phosphorylation in h-35 hepatoma cells. igf-ii receptors isolated from insulin-treated cells exhibit enhanced in vitro phosphorylation by casein kinase ii. | insulin caused a rapid, dose-dependent increase in the binding of 125i-insulin-like growth factor-ii (igf-ii) to the surface of cultured h-35 hepatoma cells. the [32p]phosphate content of the igf-ii receptors, immunoprecipitated from extracts of h-35 cell monolayers previously incubated with [32p]phosphate for 24 h, was decreased after brief exposure of the cells to insulin. analysis of tryptic digests of labeled igf-ii receptors by bidimensional peptide mapping revealed that the decrease in the ... | 1988 | 2963823 |
| evidence that the clindamycin-erythromycin resistance gene of bacteroides plasmid pbf4 is on a transposable element. | we constructed a shuttle vector, pe5-2, which can replicate in both bacteroides spp. and escherichia coli. pe5-2 contains a cryptic bacteroides plasmid (pb8-51), a 3.8-kilobase (kb) ecori-d fragment from the 41-kb bacteroides fragilis plasmid pbf4, and rsf1010, an incq e. coli plasmid. pe5-2 was mobilized by r751, an incp e. coli plasmid, between e. coli strains with a frequency of 5 x 10(-2) to 3.8 x 10(-1) transconjugants per recipient. r751 also mobilized pe5-2 from e. coli donors to bacteroi ... | 1985 | 2985540 |
| demonstration of non-degraded aleutian disease virus (adv) proteins in lung tissue from experimentally infected mink kits. brief report. | isolates of adv replicate to rather high quantities in lungs from neonatally infected mink kits. the virus was analysed for polypeptide composition, and for the first time high molecular weight polypeptides have been observed in in vivo produced advs. these polypeptides are analogous to those of in vitro produced advs. the molecular weights of the structural polypeptides of the low virulence pullman adv and the highly virulent dk and utah i isolates of adv were found to be 88kd and 78kd and in v ... | 1986 | 3002299 |
| central nervous system lesions in suckling mice and rats inoculated intranasally with sialodacryoadenitis virus. | suckling cd-1 and cfw mice and wi wistar rats were inoculated intranasally with sialodacryoadenitis virus. in animals inoculated during the first week of life, there was an acute necrotizing encephalitis with malacia and minimal inflammatory cell response. in mice and rats which survived for up to 12 days post inoculation, loss of brain substance, gitter cells and occasionally mineralized debris were seen. viral antigen was readily demonstrated in neurons and nasal epithelium in trypsin-treated, ... | 1986 | 3004003 |
| tn4351 transposes in bacteroides spp. and mediates the integration of plasmid r751 into the bacteroides chromosome. | the gene for resistance to erythromycin and clindamycin, which is carried on the conjugative bacteroides plasmid, pbf4, has been shown previously to be part of an element (tn4351) that transposes in escherichia coli. we have now introduced tn4351 into bacteroides uniformis 0061 on the following two suicide vectors: (i) the broad-host-range incp plasmid r751 (r751::tn4351) and (ii) pss-2, a chimeric plasmid which contains 33 kilobases of pbf4 (including tn4351) cloned into the incq plasmid rsf101 ... | 1986 | 3005243 |
| [morphological changes caused by adrenalectomy in rabbits]. | | 1986 | 3019628 |
| [morphological changes caused by adrenalectomy in rabbits]. | | 1986 | 3019628 |
| u3 sequences from htlv-i and -ii ltrs confer px protein response to a murine leukemia virus ltr. | human t-cell leukemia virus (htlv) types i and ii are unusual among replication-competent retroviruses in that they contain a fourth gene (chi) necessary for replication. the chi gene product, p chi, transcriptionally transactivates the viral long repeat (ltr), and is thus a positive regulator. to investigate p chi transactivation, sequences from the u3 regions of the ltrs of htlv-i and -ii were inserted into the moloney murine leukemia virus (m-mulv) ltr by recombinant dna techniques. transient ... | 1987 | 3027896 |
| facilitated transfer of incp beta r751 derivatives from the chromosome of bacteroides uniformis to escherichia coli recipients by a conjugative bacteroides tetracycline resistance element. | the broad-host-range incp beta plasmid r751 can mobilize itself from escherichia coli to bacteroides spp, but it is not maintained in bacteroides spp. if r751 carries the bacteroides transposon tn4351, it can be integrated into the bacteroides chromosome. previously we showed that r751, integrated in the chromosome of bacteroides uniformis, cannot mobilize itself out of b. uniformis into e. coli or isogenic b. uniformis strains. in this report, we showed that if the bacteroides conjugative tetra ... | 1987 | 3036772 |
| experimental models of type-i diabetes. | | 1986 | 3110756 |
| the inhibition by valproic acid of the mitochondrial oxidation of monocarboxylic and omega-hydroxymonocarboxylic acids: possible implications for the metabolism of gamma-aminobutyric acid. | the interactions of 1-5 mm valproic acid with the hepatic fatty acid oxidation are here described. valproic acid was not substrate for hepatic peroxisomal fatty acid oxidation. its activation outside the mitochondrial matrix compartment was poor when compared to that of octanoic acid, a fatty acid containing the same number of carbones. valproic acid did not inhibit the fatty acyl-coa oxidase nor the cyanide-insensitive acyl-coa oxidation. valproic acid inhibited the mitochondrial oxidations of ... | 1987 | 3117781 |
| [feed-dependent changes in the rumen mucosa of high-producing cows from the dry period to eight weeks post partum]. | | 1987 | 3122467 |
| intravenous immune globulin in symptomatic paediatric human immunodeficiency virus infection. | seven paediatric patients with symptomatic human immunodeficiency virus (hiv) infection were prospectively studied for 6-24 months after the start of intravenous immune globulin (ivig) therapy. there was substantial clinical benefit during ivig treatment with marked reduction of febrile and infectious episodes, normalization of physical and psychomotor development, and absence of mortality. the immunologic monitoring revealed some discrete objective improvements. the results of this study favour ... | 1988 | 3134244 |
| evaluation of the quality of bacteriologic methods used to diagnose tuberculosis in argentina. | | 1988 | 3147118 |
| subendocardial and subepicardial wall thickening during ischemia in exercising dogs. | to determine whether ischemia in the exercising dog is associated with preservation of subepicardial thickening relative to subendocardial thickening, 10 dogs were chronically instrumented with circumflex artery flow probes, hydraulic occluders, and pairs of ultrasonic microcrystals for determination of wall thickness in the circumflex artery distribution. one pair of crystals spanned the entire ventricular wall (transmural), and the other spanned the outer half of the ventricular wall. inner wa ... | 1988 | 3180383 |
| neuropathological definition of alzheimer disease: multivariate analyses in the morphometric distinction between alzheimer dementia and normal aging. | although establishing that a patient is suffering from dementia of the alzheimer type initially reflects a clinician's opinion, neuropathological study is for the present the most definitive examination to confirm the clinical diagnosis of alzheimer disease. we review several comprehensive publications attempting on a quantitative basis to differentiate the changes occurring with normal aging of the human brain from those indicative of alzheimer disease. new morphometric data on 5 histopathologi ... | 1988 | 3207478 |
| individual free fatty acids and lactate uptake in the human heart during severe sepsis. | coronary haemodynamics and myocardial metabolism of nonesterified fatty acids (nefa) and lactate were studied in 11 patients with severe sepsis, and compared to 10 control subjects. coronary sinus blood flow was evaluated by thermodilution. arterial and coronary sinus blood samples were collected for the measurement of lactate and total and individual nefa concentrations both in septic and control patients. there was an increase in lactate and total nefa arterial concentrations with a marked inc ... | 1988 | 3232957 |
| evaluation of the syphilis bio-enzabead assay for detection of treponemal antibody. | an enzyme-linked immunosorbent assay for treponema pallidum antibody (syphilis bio-enzabead kit; organon teknika corp., formerly litton bionetics, kensington, md.) was compared with the standard fluorescent treponemal antibody absorption test for syphilis (indirect fluorescent-antibody confirmatory test; zeus scientific inc., raritan, n.j.). six hundred specimens tested in the rapid plasma reagin card test (hynson, westcott and dunning, inc., baltimore, md.) and microhemagglutination assay for a ... | 1987 | 3294884 |
| a controlled trial of nebulized aminoglycoside and oral flucloxacillin versus placebo in the outpatient management of children with cystic fibrosis. | six children with cystic fibrosis who had persistently had pseudomonas aeruginosa isolated from their respiratory tract, completed a double-blind cross-over comparison of oral flucloxacillin and nebulized aminoglycoside versus double placebo. the patients had higher fev1 results at the end of the month of active treatment than after the month of placebo. | 1987 | 3329531 |