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current status of treatment of pneumonia.proper treatment of pneumonia is dependent upon a correct diagnosis. pneumonia may be due to infectious agents, allergic phenomena, or chemical causes. treatment regimens are outlined for the various types of pneumonia--pneumococcal, staphylococcal, fungal, and pneumonia due to gram-negative and anaerobic gram-negative bacilli, to blastomyces dermatitidis, and to the parasite pneumocystis carinii. in discussing current concepts of treatment, several well-known methods are emphasized, as well as ...1975792
some observations on the effects produced in white mice following the injection of certain suspensions of corroding bacilli.strictly anaerobic and facultatively anaerobic strains of "corroding bacilli" failed to produce any pathological symptoms when injected into white mice and no viable organisms could be recovered after 7 days. however, when these same strains were coupled with certain other living bacteria or certain sterile bacterial extracts, lesions developed from which corroding bacilli could be isolated even after 21 days.19751080
rapid screening of veillonella by ultraviolet fluorescence.among 51 strains of anaerobic gram-negative cocci belonging to the family veillonellaceae, all strains of veillonella (v. parvula and v. alcalescens) displayed red fluorescence under long-wave (366 nm) ultraviolet light, whereas no acidaminococcus or megasphaera demonstrated fluorescence. in contrast to bacteroides melaninogenicus, growth of veillonella does not require hemin and menadione, and flourescence is rapidly lost upon exposure to air. the fluorescent component of a strain of v. parvula ...19751419
the polysaccharide capsule of bacteroides fragilis subspecies fragilis: immunochemical and morphologic definition.a large-molecular-weight capsular polysaccharide was isolated from strains of bacteroides fragilis subspecies fragilis. by means of electron microscopy and staining with ruthenium red, the thick polysaccharide capsule was also visualized. with use of a radioactive antigen-binding assay, antibody to this capsular polysaccharide was demonstrated in antisera prepared in rabbits to each of eight strains of b. fragilis fragilis. antibody of similar specificity was not found in antisera prepared to ba ...19761451
survival of anaerobic bacteria during transportation. 1. experimental investigations on the effect of evacuation of atmospheric air by flushing with carbon dioxide and nitrogen.the effect of evacuation of atmospheric air during transportation on recovery of anaerobic bacteria was investigated. evacuation of atmospheric air from glass tubes by flushing with pure carbon dioxide lowered the content of oxygen to about 0.4 per cent. three b. fragilis strains and one strain of fusobacterium mortiferum and of peptostreptococcus anaerobius were investigated. bacterial recovery was determined one hour and 24 hours after evacuation of atmospheric air by pure carbon dioxide and p ...19763089
vitamin b12 absorption--a study of intraluminal events in control subjects and patients with tropical sprue.the intraluminal fate of orally administered radioactive vitamin b12 has been studied in control subjects with normal vitamin b12 absorption and those with vitamin b12 malabsorption due to tropical sprue. in control subjects 1 to 21% of the dose was bound to sedimentable material and 37 to 75% was bound to immunoreactive intrinsic factor. in subjects with vitamin b12 malabsorption due to tropical sprue, the results were identical with the control subjects. bacteriological studies showed a statis ...19753457
in vitro susceptibility studies with josamycin and erythromycin.the in vitro activity of josamycin and erythromycin against five bacterial species was compared. in general, erythromycin was slightly more active by weight than josamycin, although both agents had a similar range of activity.19764012
description of a polyvalent conjugate and a new serogroup of bacteroides melaninogenicus by fluorescent antibody staining.a polyvalent conjugate (fluorescein isothiocyanate-labeled antibody reagent) containing serogroups a, b, and c conjugates was prepared. this polyvalent conjugate gave a positive fluorescent antibody (fa) stain with 49 stains of bacteroides melaninogenicus representing serogroups a, b, and c. when additional strains (92 strains) of the three subspecies of b. melaninogenicus were examined by the fa stain, with a, b, and c, and polyvalent conjugates, nine strains of b. melaninogenicus subsp. interm ...19766487
in vitro activity of josamycin against aerobic gram-positive cocci and anaerobes.josamycin, a new macrolide antibiotic, was compared with ampicillin, erythromycin, and clindamycin in vitro against 25 isolates each of pneumococci, enterococci, staphylococcus aureus, s. epidermidis, and nonenterococcal hemolytic streptococci and against 25 anaerobes including 10 bacteroides fragilis. minimal inhibitory concentration and minimal bactericidal concentration data were obtained for the aerobic organisms, using serial twofold tube dilutions in mueller-hinton broth. minimal inhibitor ...19767189
formation of beta-lactamase in bacteroides fragilis: cell-bound and extracellular activity.nine strains of bacteroides fragilis were cultivated in stirred fermentors and tested for their ability to produce beta-lactamase. there was a correlation between formation of beta-lactamase and high values of the minimal inhibitory concentration against beta-lactam antibiotics. b. fragilis strain b70 was used for optimizing the production of beta-lactamase. the highest bacterial yield was obtained in a proteose peptone-yeast extract medium. optimal conditions for growth and beta-lactamase produ ...19767997
influence of pencillinase production by strains of bacteroides melaninogenicus and bacteriodes oralis on pencillin therapy of an experimental mixed anaerobic infection in mice. 19768031
influence of amino acids on the growth of bacteroides melaninogenicus.addition of individual amino acids to a trypticase-yeast extract-hemin medium affected growth rates and final yields of an asaccharolytic strain and a saccharolytic strain of bacteroides melaninogenicus. l-aspartate or l-asparagine produced maximal growth enhancement for both strains. l-14caspartate was fermented by resting cells of the asaccharolytic strain. l-cysteine or l-serine also enhanced growth for the saccharolytic strain. however, growth of the saccharolytic strain was inhibited by l-l ...19768425
bifidobacteria in the intestinal tract of infants: an in-vivo study.weekly faecal specimens from 18 babies were examined during the first 8 weeks of life. eight infants were breast fed, ten were bottle-fed. all suckling infants received supplementary feeds for the first 8 days. a buffer consisting of acetic acid and acetate was demonstrated in the faeces of all the breast-fed infants at some time during the period of examination. this buffer was rarely detected during the 1st week of life when supplementary feeds were given, and buffer already present gradually ...19768646
blastogenic response of human lymphocytes to oral bacterial antigens: comparison of individuals with periodontal disease to normal and edentulous subjects.cell-mediated immunity in humans to antigens derived from oral plaque bacteria was investigated by using the lymphocyte blastogenesis assay. subjects with varying severities of periodontal disease including normal, gingivitis, periodontitis, and edentulous were compared. mononuclear leukocytes were separated from peripheral blood and cultured with antigens prepared by sonication of actinomyces viscosus (av), actinomyces naeslundii (an), veillonella alcalescens (va), leptotrichia buccalis (lb), b ...197610259
characterization of bacteroides melaninogenicus.fifty-eight human isolates of bacteroides melaninogenicus, 42 from a variety of clinical infections and the rest from normal flora, were studied for pigment production and ultraviolet light fluorescence and by forty biochemical and other tests, including end-product analysis by gas-liquid chromatography. in a number of instances, tests were repeated several times and the results were reproducible. agar plate dilution susceptibility tests were also performed to 12 antimicrobial agents. these 58 s ...197610317
bacterial interference by oropharynegeal and clinical isolates of anaerobic bacteria.anaerobic isolates were tested for bacterial inhibitory activity. of 144 isolates, 102 were from oropharynegeal washings, and 42 were from clinical specimens. thirteen facultative bacterial species (seven members of the enterobacteriaceae and six species of gram-positive cocci) were used as indicators of inhibition. eleven anaerobic species were isolated from oral secretions. all isolates of bacteroides melaninogenicus, the most commonly recovered species, consistently inhibited several species ...197610336
purification and characterization of bile salt hydrolase from bacteroides fragilis subsp. fragilis.a high-molecular-weight (250 000) bile salt hydrolase (cholylglycine hydrolase, ec 3.5.-.-) was isolated and purified 128-fold from the "spheroplast lysate" fraction prepared from bacteroids fragilis subsp. fragilis atcc 25285. the intact enzyme had a molecular weight of approx. 250 000 as determined by gel infiltration chromatography. one major protein band, corresponding to a molecular weight of 32 500, was observed on 7% sodium dodecyl sulfate polyacrylamide gel electrophoresis of pooled frac ...197610993
susceptibility of phosphomycin as a differential character for gram negative anaerobic bacilli. 197611203
an unusual bilateral pathological fracture.an unusual case of a bilateral pathological fracture of the mandible has been presented. the fracture of the right side of the mandible was through an area of bacteroides osteomyelitis and the fracture of the left side of the mandible was through a large cementifying fibroma, which had replaced the left body of the mandible.197711280
the role of microflora in development of graft-versus-host disease. 197611583
casamino acids enhance growth of bacteroides melaninogenicus.casamino acids enhance the growth of bacteroides melaninogenicus when added to various concentrations of trypticase. absence of a peptide, not amino acids, is responsible for the inability of casamino acids to support growth.197712141
a study of the pigment produced by bacteroides melaninogenicus. 197612196
isolation of 3-phosphohistidine from phosphorylated pyruvate, phosphate dikinase.pyruvate, phosphate dikinase (ec 2-7-9-1) catalyzes formation of phosphoenolpyruvate, amp, and inorganic pyrophosphate from pyruvate, atp, and orthophosphate. a pyrophosphoryl and phosphoryl form of the enzyme is involved in this transfer. the 32pphosphoryl form of pyruvate, phosphate dikinase was prepared with enzyme isolated from bacteroides symbiosus. the 32pphosphoryl enzyme was found to have properties corresponding to a phosphoramidate linkage and this was confirmed by isolation of 3-32pph ...197612506
septicemia caused by ristella. 197612929
beta-lactamase activity in strains of bacteroides melaninogenicus and bacteroides oralis.beta-lactamase from strains of bacteroides melaninogenicus and bacteroides oralis hydrolyzed penicillin more rapidly than ampicillin or carbenicillin. cephalothin and a chromogenic cephalosporin (87/312) were also hydrolyzed by the enzyme. activity was found only in beta-lactam-resistant strains, but there was considerable variation in activity among strains having the same minimal inhibitory concentrations of antibiotic. beta-lactamase activity was cell bound and appeared to be tightly associat ...197713705
fermentation of l-aspartate by a saccharolytic strain of bacteroides melaninogenicus.resting cells of bacteroides melaninogenicus fermented l-14caspartate as a single substrate. the 14c-labeled products included succinate, acetate, co2, oxaloacetate, formate, malate, glycine, alanine, and fumarate in the relative percentages 68, 15, 9.9, 2.7, 1.8, 1.0, 0.7, 0.5, and 0.06, respectively, based on the total counts per minute of the l-14caspartate fermented. ammonia was produced in high amounts, indicating that 96% of the l-aspartate fermented was deaminated. these data suggest that ...197713713
comparison of the biochemical properties of bacteroides melaninogenicus from human dental plaque and other sites. 197614095
purification and some properties of an extracellular alpha-amylase from bacteroides amylophilus.a medium was developed to obtain maximum yields of extracellular amylase from bacteroides amylophilus 70. crude enzyme preparation, obtained by ammonium sulfate precipitation of cell-free broth, contained six amylolytic isoenzymes that were detected by isoelectric focusing and polyacrylamide gel electrophoresis. one of these amylases was purified by diethylaminoethyl-sephadex a-50 ion-exchange chromatography and sephadex g-200 gel filtration techniques. some properties of the purified extracell ...197714926
treatment of bacteroides endocarditis with carbenicillin.a heroin addict developed acute bacterial endocarditis with streptococcus viridans and bacteroides melaninogenicus. although blood cultures became negative during penicillin g and clindamycin therapy there was little clinical response. prompt clinical improvement was achieved with intravenous carbenicillin in a dose of 40 g daily. in vitro testing supported the superiority of carbenicillin therapy in this patient.197715455
the syndrome of inappropriate secretion of antidiuretic hormone associated with anaerobic thoracic empyema.the syndrome of inappropriate secretion of antidiuretic hormone has been associated with many pulmonary diseases, including tuberculosis and bacterial and viral pneumonia: however, it has not been reported with anaerobic infections or empyema in the absence of pneumonia. we report a patient with empyema due to bacteroides melaninogenicus, bacteroides oralis, and peptostreptococcus who developed the syndrome. eight hours before the start of therapy, his serum sodium concentration was 127 meq per ...197715491
energy-dependent incorporation of sphingolipid precursors and fatty acids in bacteriodes melaninogenicus.washed cells of bacteroides melaninogenicus are unable to incorporate the sphingolipid precursor 3-ketodihydrosphingosine (3kds) or dihydrosphingosine into the complete sphingolipids ceramide phosphorylethanolamine (cpe) and ceramide phosphorylglycerol (cpg), whereas growing cultures are able to do so. this result suggested that an energy source was required by washed cells to initiate the incorporation of 3kds. investigation of a number of energy sources for b. melaninogenicus showed that gluta ...197715984
purification and immunochemical characterization of the outer membrane complex of bacteroides melaninogenicus subspecies asaccharolyticus.morphologic study of bacteroides melaninogenicus subspecies asaccharolyticus by electron microscopy disclosed the presence of a capsule and a cell wall structure otherwise typical of a gram-negative organism. an outer membrane complex was isolated with use of gentle methods. relative purity of the preparation was confirmed by electron microscopy and by the formation of a single band in a sucrose density gradient. gel chromatography was used for separation of the major components of the membrane. ...197716065
penicillin resistance and penicillinase production in clinical isolates of bacteroides melaninogenicus.the minimum inhibitory concentrations (mic) of penicillin and six other antimicrobials were determined for 50 clinical isolates of bacteroides melaninogenicus. agar dilution susceptibilities were performed using supplemented brucella blood agar and the proposed national committee for clinical laboratory standards standard method for anaerobes; results with the two methods were comparable. a penicillin concentration >/=0.8 mug/ml was needed to inhibit 56% of the isolates, whereas 100% were suscep ...197716564
the classification of bacteroides melaninogenicus and related species. 197717592
activity of spectinomycin against anaerobes.the in vitro inhibitory activity of spectinomycin was tested against various anaerobic bacteria. different results were obtained with different media and with different initial ph's of the media. the highest minimum inhibitory concentrations for bacteroides fragilis (formula: see text 128 mug/ml) were obtained with the use of wilkins-chalgren agar (ph 7.2) and brucella blood agar (ph 7.0). brucella blood agar at higher ph's (7.4 and 8.0) and mueller-hinton and diagnostic sensitivity test agars p ...197718985
antimicrobial activity of human ascitic fluid. 197719339
chemical composition, serological reactivity and endotoxicity of lipopolysaccharides extracted in different ways from bacteroides fragilis, bacteroides melaninogenicus and bacteroides oralis.lipopolysaccharides (lps) extracted from strains of bacteroides fragilis, bacteroides melaninogenicus and bacteroides oralis with phenol-water, trichloroacetic acid, edta or liquid phenol-chloroform-petroleum ether (pcp) and isolated by ultracentrifugation, varied considerably in their quantitative chemical composition. negligible yields of lps were obtained by pcp-extraction. all preparations were more or less serologically active. all methods (except pcp) extracted the same o-antigenic determi ...197719922
sodium dodecyl sulphate-polyacrylamide gel electrophoresis of polypeptides from bacteroides melaninogenicus. 197722532
current aspects of gas gangrene, apropos of 47 cases collected over a 3-year period (1974-1976).on the basis of 47 cases of gas gangrene collected over the three year period between 1974 and 1976, the authors review the circumstances surrounding its development, the clinical features and the prognosis of the disorder which remains grave despite a well-defined therapeutic protocol combining surgery, antibiotics and hyperbaric oxygen. there would appear to be a real resurgence of the disease at the present time. post-traumatic and surgical aetiologies predominate, giving rise to two types of ...197723023
bacteroides fragilis septicemia.on the basis of eight cases of bacteroides fragilis septicaemia, the authors review recent data from the literature related to this topic. they first note the increasing prevalence, accounting for approximately 10 per cent of all septicaemias at the present time. they note that the most frequent portals of entry are sites of localised suppuration, related on the one hand to digestive or gynaecological surgery and, secondly, to the post-partum or post-abortum period. from a therapeutic standpoint ...197723026
role of nucleosides, 5-phosphoribosyl-1-pyrophosphate, and ribose 1-phosphate in the biosynthesis of phosphosphingolipids in bacteroides melaninogenicus. 197823730
local antibody production in guinea pigs by gingival inoculation of bacteroides melaninogenicus. 197523908
attachment of bacteroides melaninogenicus subsp. asaccharolyticus to oral surfaces and its possible role in colonization of the mouth and of periodontal pockets.this investigation examined the ability of cells of bacteroides melaninogenicus subsp. asaccharolyticus 381 to adhere to surfaces that might be important for its initial colonization of the mouth and its subsequent colonization in periodontal pockets. of 48 asaccharolytic strains of b. melaninogenicus, 47 agglutinated human erythrocytes, whereas none of 20 fermentative strains, which included reference cultures of the subspecies intermedius and melaninogenicus, were active. electron microscopy i ...197824002
the hydrolysis of dextran by gram negative non-sporing anaerobic bacilli. 197724035
quantitative evaluation of three commercial blood culture media for growth of anaerobic organisms.the ability of three different commercial blood culture media--brain heart infusion broth (pfizer), thiol broth (difco), and prs-peptone broth (becton, dickinson & co.)--to support the growth of five different anaerobes is described. inocula of 100 and 1,000 colony-forming units per ml were used to evaluate potential differences in survival, lag time, growth rate, and doubling times of each anaerobe in each medium. in addition, each medium was evaluated for its ability to neutralize the antibact ...197824059
immunochemical and biologic studies of the lipopolysaccharide of bacteroides melaninogenicus subspecies asaccharolyticus. 197824073
clotting and fibrinolytic activities of bacteroides melaninogenicus.106 strains of bacteroides melaninogenicus ss. asaccharolyticus, intermedius and melaninogenicus were tested for production of extracellular blood clotting and fibrinolytic factors. 78.3% of tested strains caused clotting of rabbit plasma and 79.2% activated human plasminogen. strains producing the clotting or fibrinolytic factor only were isolated. both factors were quite active, as positive results for most strains were detected within one hour of incubation in controlled test system.197724963
the nature of clotting and fibrinolytic activities of bacteroids melaninogenicus.from the supernatant of b. melaninogenicus ss. asaccharolyticus culture, a protein fraction was isolated by ethanol precipitation. the fraction was tested for the presence of clotting and fibrinolytic activities by application of quantitative techniques and specific substrates for measurement of prothrombin and plasminogen activation, and collagenase and elastase activity. it is postulated that ability of bacteroides melaninogenicus ss. asaccharolyticus extracellular factors to clot fibrinogen a ...197824965
formation of glycosidases in batch and continuous culture of bacteroides fragilis.nine strains of bacteroides fragilis were cultivated in stirred fermentors and tested for their ability to produce glycosidases. b. fragilis subsp. vulgatus b70 was used for optimizing the production of glycosidases. the highest bacterial yield was obtained in proteose peptone-yeast extract medium. the optimum ph for maximal bacterial yield was 7.0, and the optimum temperature for growth was 37 degrees c. the formation of glycosidases was optimal between ph 6.5 and 7.5, and the optimum temperatu ...197825044
reduction of azo dyes by intestinal anaerobes.reduction of seven azo dyes (amaranth, ponceau sx, allura red, sunset yellow, tartrazine, orange ii, and methyl orange) was carried out by cell suspensions of predominant intestinal anaerobes. it was optimal at ph 7.4 in 0.4 m phosphate buffer and inhibited by glucose. flavin mononucleotide caused a marked enhancement of azo reduction by bacteroides thetaiotaomicron. other electron carriers, e.g., methyl viologen, benzyl viologen, phenosafranin, neutral red, crystal violet, flavin adenine dinucl ...197825047
the bactericidal activity of nitrofurantoin and metronidazole against anaerobic bacteria. 197825874
the cellular hypersensitivity in guinea pigs immunized with bacteroides melaninogenicus through gingiva. 197626475
efficient fractionation of collagenolytic sample from oral bacteroides melaninogenicus. 197626476
bacteriocin-like activities of human dental plaque flora against oral anaerobic microorganisms. 197726477
a method for the isolation of bacteroides melaninogenicus from the human mouth.isolation procedures involving the direct plating of specimens and the use of selective, non-selective, transport and enrichment media were compared in respect of their value for the recovery of bacteroides melaninogenicus from the gingival sulcus. a selective medium containing kanamycin and vancomycin enhanced the recovery rate of b. melaninogenicus. vmgii transport medium was convenient to use and gave as good a recovery rate as that obtained with plates directly inoculated in the clinic.197826805
isolation and characterization of bacteroides melaninogenicus from human specimen (author's transl). 197827649
early diagnosis of crepitant gangrene caused by bacteroides melaninogenicus.a rapid non-invasive test for the presence of b. melaninogenicus in the wounds of crepitant non-clostridial gangrene is described. the wounds are viewed under an ultraviolet light, and the presence of bright red fluorescene indicates the probable presence of b. melaninogenicus.197827832
an experimental model for osteomyelitis in the mandible. 197728338
beta-lactamases in bacteroides.bacteroides fragilis is responsible for most anaerobic infections in man. most isolates of b. fragilis show resistance to beta-lactam antibiotics. this resistance might be due to beta-lactamase production or permeability barrier in the cell wall. b. fragilis produce beta-lactamase with mainly cephalosporinase activity. other bacteroides species such as b. clostridiformis, b. melaninogenicus and b. oralis also produce beta-lactamase but with different biochemical characteristics.197828561
chloramphenicol acetyltransferase of bacteroides fragilis.chloramphenicol-resistant strains of bacteroides fragilis (minimum inhibitory concentration, 12.5 mug/ml) were isolated from a stool specimen which contained multiply resistant escherichia coli. the enzyme responsible for resistance, chloramphenicol acetyltransferase, was produced constitutively by these strains; the specific activity was 10-fold lower than that of the e. coli enzymes. similar activity was not detected in susceptible b. fragilis strains, nor could it be induced by growth in the ...197828690
cefoxitin sodium activity against anaerobes: effect of the inoculum size, ph variation and different culture media. 197829027
pleuropulmonary lung infection by anaerobic bacteria.two patients with severe pleuropulmonary infection caused by non-sporing anaerobic bacteria are described. one had an empyema with foul-smelling pus and developed bacteraemia, and the other developed a lung abscess. both were successfully treated with antibiotics and drainage, chest surgery being avoided. the successful diagnosis and treatment of these patients involved close liaison between clinical and laboratory staff.197829654
preparation of prereduced anaerobically sterilized media and their use in cultivation of anaerobic bacteria.several modifications of the roll-tube method have made it simpler for routine use in the isolation and growth of anaerobic bacteria. these include use of a check valve for the production of prereduced anaerobically sterilized media; a salvarsan tube under oxygen-free gas pressure for the dispensing of molten prereduced anaerobically sterilized agar medium; a kelly infusion bottle with a graduated pipette side arm (also under gas pressure) for quantitative delivery of fluid prereduced anaerobica ...197829909
[ecology of bacteroides melaninogenicus in the oral cavities of preschool children (author's transl)]. 197731099
growth inhibition of streptococcus mutans by the black pigment (haematin) of bacteroides melaninogenicus. 197831156
acute suppurative parotitis caused by anaerobic bacteria: report of two cases.staphylococcus aureus is the predominant pathogen in acute suppurative parotitis. although anaerobic bacteria are prevalent in the normal oral and upper respiratory tract flora and frequently are involved in oral and facial infections, they have seldom been isolated from patients with suppurative parotitis. it may be that a role for anaerobes in parotitis has not been documented because of inadequate specimen transport or anaerobic culture techniques. we describe two cases of acute suppurative p ...197832513
sphingolipid biosynthesis and vitamin k metabolism in bacteroides melaninogenicus.b. melaninogenicus provides a unique system for the study of the biosynthesis of an important group of lipids, the phosphosphingolipids. sphingolipid biosynthesis can be repressed and induced by depletion and restoration of vitamin k. at least one enzyme involved in sphingolipid biosynthesis from the microorganism can be solubilized and so purified by conventional methods. pathways involved in biosynthesis may differ from hitherto postulated pathways, for example, the incorporation of nh4+ into ...197932765
metronidazole in the prophylaxis and treatment of anaerobic infection.the influence of prophylactic metronidazole on vaginal carriage rates of anaerobes and the development of postoperative anaerobic infection was studied in 104 women who underwent abdominal hysterectomy. metronidazole prophylaxis in 54 patients led to a decrease in the anaerobe vaginal carriage rate from 65% pre-operatively to 17% and 28% on the 3rd and 7th postoperative days respectively. in the control group (50 patients) no significant decrease in anaerobe yield was noted, corresponding percen ...197833454
complement activation by propionibacterium acnes and bacteroides melaninogenicus. 197833641
longitudinal study of experimentally induced periodontal disease in macaca arctoides: relationship between microflora and alveolar bone loss.macaca arctoides monkeys develop periodontal disease, and they harbor a periodontopathic indigenous flora largely similar to that of humans. this study showed that various haemophilus isolates and h2o2-splitting asaccharolytic bacteroides melaninogenicus strains constituted major segments of the monkey periodontal microflora. these organisms have not been previously identified among human isolates. furthermore, the present data revealed that asaccharolytic b. melaninogenicus strains increased in ...197933902
chromogenic cephalosporin spot test to detect beta-lactamase in clinically significant bacteria.lactamase production. reacteroides melaniongenicus, 14197934624
serological identification of bacteroides species by an enzyme-linked immunosorbent assay.an enzyme-linked immunosorbent assay was used to titrate antisera raised against live cultures of eight type species (biotypes) of bacteroides with the edta-released outer-membrane complex from 29 characterised strains of bacteroides species. with only minor exceptions, the strains investigated reacted to titre with the antisera raised against the homologous type species and not against the heterologous type species. cross-reactivity between heterologous species and antiserum was only significan ...197934633
factors affecting the incidence and anti-salmonella activity of the anaerobic caecal flora of the young chick.thirty-two different types of anaerobic bacteria isolated from chickens have been tested for anti-salmonella activity in vitro. under the conditions of the test only bacteroides hypermegas and a bifidobacterium sp. were shown to inhibit the salmonellas and this was attributed to the production of volatile fatty acids (vfa's) coupled with a low ph. when these organisms were tested in newly hatched chicks no inhibition of s. typhimurium occurred. possible explanations for this observation are cons ...197934658
[phlegmon of the fore arm succeeding to a dog-bite : respective role of the miscellaneous bacteria involved in this mixed infection (author's transl)].the authors report a case of a severe mixed infection (phlegmon of the fore arm) succeeding to a dog bite. a complex flora has been isolated from this phlegmom : pasteurella multocida, pasteurella pneumotropica, streptococcus mitis, actinomyces sp., bacteroides melaninogenicus, and a gram- bacteria related to group ii j. the pathogeny of such diseases is discussed, dwelling in one hand on the part of synergistic development of these various germs and in the other hand on the part played by saliv ...197934886
[bacteriological studies of peritonitis].bacterial infections of the peritoneum may be primary (pneumococcal peritonitis of childhood). these are rare, as are streptococcal and staphylococcal peritonitis in the neonate, following umbilical or tegumental infections. the great majority of cases of peritonitis are secondary to an adjacent inflammation, a perforation of the gastro-intestinal tract, or trauma, which may be surgical in origin. the microbial flora involved is generally mixed, with a high proportion of anaerobic organisms, ref ...197835067
[antibiotic treatment in acute peritonitis].the selection depends on several factors and in particular: -the means of transport by which the antibiotic reaches the peritoneum : the aminosides and the betalactamines attain the peritoneum easily while the polymyxins do not; -the causative agent: most often it is a gram negative aerobic or anaerobic bacteria. the entero-bacteria predominate but in the last few years bacteroides fragilis has been frequently encountered giving rise to numerous studies. the flora is most often polymicrobic. th ...197835071
urease-producing species of intestinal anaerobes and their activities.urease activities of anaerobic bacteria that constituted predominant gut flora were examined. it was demonstrated that some strains of eubacterium aerofaciens, e. lentum, and peptostreptococcus products produced urease. they were the most numerous species in human feces. all strains of bifidobacterium infantis and some strains of bacteroides multiacidus, b. bifidum, clostridium symbiosum, fusobacterium necrophorum, f. varium, lactobacillus fermentum, peptococcus asaccharolyticus, and p. prevotii ...197936839
intracellular polysaccharide of bacteroides fragilis.formation of iodophilic polysaccharide (ips) from glucose was demonstrated in 27 strains of bacteroides fragilis. synthesis was dependent on the glucose concentration of the medium, the ph and the growth phase. when glucose was in short supply the cellular polysaccharide was degraded rapidly at ph 4.5 to 6.5 and fatty acids accumulated in the medium. storage of ips was not responsible for the low carbon recoveries observed in fermentation balance studies. in electron micrographs of thin sections ...197937285
antibiotic susceptibility of anaerobic bacteria with special reference to bacteroides fragilis.it was shown that recent swedish clinical isolates of anaerobic bacteria are susceptible to many antibiotics by the agar dilution method with the exception of the bacteroides group versus beta-lactam antibiotics or tetracyclines. strains of b. fragilis were inhibited by 4--greater than 128 micrograms benzylpenicillin or cephalothin/ml, 1.0--64 micrograms cefoxitin/ml, 0.064--2 micrograms clindamycin or metronidazole/ml, 2--8 micrograms chloramphenicol/ml, 2--16 micrograms fusidic acid/ml and 0.0 ...197937593
anaerobic pulmonary infections.the main cause of anaerobic pulmonary infections is aspiration of saliva, upper airway secretions or gastric content. predisposing conditions include prominent dental disease, chronic upper respiratory tract infections and reduced consciousness. fusobacterium nucleatum, bacteroides melaninogenicus and anaerobic gram-positive cocci are the most frequently encountered organisms. the clinical presentations are lung abscess, lung gangrene and empyema, which all tend to take a slow and indolent cours ...197937594
[in vitro ability of dental plaque formation by bacteroides melaninogenicus (author's transl)]. 197738768
[anaerobic gram negative septicemias in digestive tract surgery and in surgical intensive care]. 197939472
the porphyrin pigmentation of subspecies of bacteroides melaninogenicus.various subspecies of bacteroides melaninogenicus differ in their pigmentation. subsp. asaccharolyticus produces protohaem almost exclusively, subsp. intermedicus both protohaem and a smaller proportion of protoporphyrin, and subsp. melaninogenicus mainly protoporphyrin with a trace of protohaem. as a consequence young colonies can be differentiated by their red fluorescence in u.v. light (365nm): subsp. asaccharolyticus does not fluoresce, subsp. intermedicus shows a limited fluorescence, and s ...197939545
aerobic and anaerobic cervical flora of healthy thai women.the bacteriologic study of the cervical flora in 50 healthy women revealed that 70% harbored mixed aerobic and anaerobic organisms. aerobic organisms alone were recovered in 15 women (30%). polymicrobial organisms were found in all but five women (in these only one aerobe was isolated per patient). the majority of patients had 1-2 anaerobes, with more than two aerobes. the common aerobes were alpha-hemolytic streptococci, staphylococcus epidermidis and lactobacilli. the most common anaerobes wer ...197839833
characterization of anaerobic gram-negative bacilli by using rapid slide tests for beta-lactamase production.a total of 175 isolates of anaerobic gram-negative bacilli were tested for beta-lactamase production by using a slide test modification of the chromogenic cephalosporin (nitrocefin, glaxo, middlesex, england) assay and the iodometric slide test. included isolates were bacteroides melaninogenicus (46), b. fragilis (78), other bacteroides isolates (21), fusobacterium (25), and other gram-negative bacilli (5). both slide tests detected 25 b. melaninogenicus isolates that were beta-lactamase produce ...197941001
gas abscess transformation of a huge hypernephroma. 197941445
anaerobic isolates in chronic recurrent suppurative otitis media. treatment with carbenicillin alone and in combination with gentamicin.tympanocentesis was performed in 32 pediatric patients with chronic recurrent suppurative otitis media. the aspirate was cultured aerobically and anaerobically. aerobes were isolated from ten patients (31.2%); anaerobes from one patient; and both aerobes and anaerobes from 21 patients (65.6%). there were 46 aerobic isolates. the aerobes commonly recovered were pseudomonas aeruginosa (24 isolates) proteus sp. (5) and staphylococcus aureus (3). there were 32 anaerobes isolated including anaerobic ...197941811
fluorescent antibody test kit for rapid detection and identification of members of the bacteroides fragilis and bacteroides melaninogenicus groups in clinical specimens.the fluoretec fluorescent antibody test kit (pfizer inc., new york, n.y.), developed for the rapid detection of members of the bacteroides fragilis and b. melaninogenicus groups, was evaluated by testing 58 stock cultures and 76 clinical specimens. the test reagents detected 100% of 40 b. fragilis and b. thetaiotaomicron stock culture strains, although only 22% of 18 b. vulgatus, b. distasonis, and b. ovatus strains showed positive fluorescence. the 76 clinical specimens were evaluated by examin ...197941847
rapid diagnosis of anaerobic infections by gas-liquid chromatography.it was postulated that the short chain fatty acids (scfa) produced by anaerobic bacteria might serve as microbial markers in purulent material. eighteen pus specimens from various sources were analysed by gas-liquid chromatography (glc), and the scfa detected were compared with the microorganisms isolated by conventional methods. it was found that the detection of propionic, isobutyric, butyric, or isovaleric acids by direct glc of pus specimens is strong evidence for anaerobic infection but not ...197941850
the effect of ph on maximum bacterial growth rate and its possible role as a determinant of bacterial competition in the rumen. 197943321
purification of pili from bacteroides nodosus and an examination of their chemical, physical and serological properties.pili from bacteroides nodosus were purified to greater than 99% homogeneity by precipitation at ph 4.0 and in mgcl2 followed by chromatography on biogel a150. the pili were composed entirely of one type of polypeptide subunit, pilin. no carbohydrates, nucleic acid, lipid, lipopolysaccharide or phosphate could be detected in purified pili preparations. the molecular weight of pilin from b. nodosus strains 91b and 198 was 18,400 and from strain 80 was 19,300. the isoelectric points of pili from b. ...197943354
factors affecting digestion of grain-supplemented straw. 197943698
the normal microbial flora of the baboon vagina.the microbial flora of the upper vagina and cervix was examined in 38 adult baboons at various stages of the menstrual cycle. the mean number of different species isolated from each baboon was 9.5, with species of bacteroides, corynebacterium and group d streptococci predominating. lactobacilli and mycoplasmas were found in 47.4 and 44.7% of the animals, respectively. no ureaplasms were isolated. cyclical variations in the microbial flora were minimal.197943896
intestinal microflora and bile acids. in vitro cholic acid transformation by mixed fecal culture of rats.in vitro cholic acid (ca) transformation by mixed fecal culture was investigated. concentrations of glucose, peptone, and yeast extract in the medium and the initial ph of the medium markedly affected the ca transformation. yeast extract enhanced the transformation, whereas high concentrations of glucose and peptone inhibited it. when the initial ph of the medium was below 6.5, ca was converted to 7-keto-deoxycholic acid (7kd), and formation of deoxycholic acid (dc) was not observed. in contrast ...197943948
detection of anticapsular antibodies to bacteroides asaccharolyticus in serum from rabbits and humans by use of an enzyme-linked immunosorbent assay.a sensitive serologic test, the enzyme-linked immunosorbent assay (elisa), was used to detect serum igg antibodies directed specifically to a capsular antigen of bacteroides asaccharolyticus (previously known as bacteroides melaninogenicus subspecies asaccharolyticus). anticapsular igg was measured in 30 specimens of rabbit serum after the animals were immunized with whole b. asaccharolyticus, the two subspecies of b. melaninogenicus, and several other bacterial species. species-specific anticap ...197944309
comparative ultrastructure of bacteroides melaninogenicus subspecies. 197944319
black-pigmented bacteroides from clinically characterized periodontal sites. 197944523
immunochemical characterization of surface antigens of bacteroides melaninogenicus.major surface antigens of bactmbrane complex by gentle methods, purified, and characterized immunochemically. a lipopolysaccharide (lps) was found to be chemically distinct from the lps of facultative gram-negative bacteria in that it lacked two core sugars, 2-keto-3-deoxyoctonate and heptose, as well as beta-hydroxymyristic acid, the predominant fatty acid in the lipid a moiety. the lps was further atypical in that it had a very low level of biologic activity. a capsular polysaccharide was demo ...197944922
the role of bacteroides melaninogenicus and other anaerobes in periodontal infections.recent taxonomic and anatomical studies of dental plaque associated with periodontal health and disease have demonstrated that differences in the microbial populations in plaque may be responsible for the initiation and progression of disease. the consistent isolation of large numbers of anaerobic and capnophilic bacteria from the depths of periodontal lesions has suggested an important role for these organisms. bacteria that have been isolated include capnocytophaga (bacteroides ochraceus), oth ...197944923
virulence factors of anaerobic bacteria: summary of the symposium. 197944924
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