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in vitro effect of leukocytic and thymic histones and their fractions on the activity of the causative agent of meningopneumonia.it was found in studying the antimeningococcus activity of the leukocytic and thymus histones and their fractions that both histones were capable of neutralizing in vitro the activity of the causative agent of meningopneumonia (mp). the neutralization effect was chiefly associated with the f3 fraction rich in arginine and depended on the duration of the histone fraction contact with the mp causative agent, the weight concentration of the histone and the ph of the incubation medium.19751947
factors affecting the sensitivity of replicating mccoy cells in the isolation and growth of chlamydia a (tric agents).normal non-irradiated mccoy cell cultures provide a sensitive and reproducible method for the isolation of oculo-genital strains of chlamydia a directly from human secretions and for laboratory studies with these agents. since september 1973, chlamydia have been isolated from 175 of 562 women (32-1%) attending venereal disease clinics. freshly isolated and low passage strains have been used to determine the importance of centrifugation, constitution and ph of the tissue culture medium, and the t ...19766592
phagocyte lysosomes: interactions with infectious agents, phagosomes, and experimental perturbations in function. 197720833
seroepidemiological investigations in domestic ruminants from egypt, somalia and jordan for the demonstration of complement fixing antibodies against rickettsia and chlamydia (author's transl).1450 random serum samples of domestic ruminants from egypt, somalia and jordan were investigated for complement fixing antibodies against rickettsia and chlamydia. between 1.5 and 3.4% of the samples from the animals investigated had antibodies against the rmsf-group of rickettsia, with exception of the sera from somalian cattle and sheep from jordan. antibodies against rickettsia of the typhus-group were found in 4 cattle and 1 goat from jordan and 2 sheep from egypt; by agglutination test with ...197828651
[imported diseases from the viewpoint of the microbiologist]. 197831553
a comparison of staining techniques for demonstrating group a chlamydia in tissue culture. 197551467
antigenic analysis of chlamydiae by two-dimensional immunoelectrophoresis. ii. a trachoma-lgv-specific antigen.two-dimensional immunoelectrophoresis was utilized to study precipitins in hyperimmune rabbit serum made against chlamydiae and from patients with chlamydial infections. an antigen of triton x-100-solubilized l2/434/bu organisms with an electrophoretic mobility of 0.65 relative to bovine serum albumin at ph 8.6 was excised from the agarose gel of electrophorograms as antigen-antibody complexes and used to immunize rabbits. a monospecific antiserum to antigen 0.65 was obtained that reacted with t ...197551883
[identification of the causative agents of trachoma and paratrachoma by the method of direct immunofluorescence and staining by the romanovskiÄ­-giemsa method]. 197656498
[biology of a newly isolated agent of the chlamydia group].the results of identification of the agent ("progress" strain) isolated from the organs of a calf sacrificed in the early stage of pneumonia are presented. the agent adapted to yolk sacs of chick embryos was pathogenic for white mice by the intracerebral route and for monkeys infected with aerosol. the infected animals developed specific complement-fixing antibody. the antigen of the agent reacted in the cft with homologous immune sera and with sera against ornithosis and other members of the or ...197556815
letter: search for microbes in arthritis. 197657384
letter: growth of chlamydia trachomatis in cell culture. 197657430
comparison of chlamydia subgroup a detection from clinical specimens after 40 and 64 hours of incubation in 5-iodo-2-deoxyuridine-treated mccoy's cells.the time course of formation of inclusion bodies produced by chlamydia in 5-iodo-2-deoxyuridine (iudr)-treated mccoy's cells was studied with the use of a known isolate of chlamydia trachomatis d/uw-184/ur and 47 frozen clinical urethral specimens previously shown to be either positive or negative for chlamydial inclusions after 3 days of incubation. subsequent examination of 369 clinical specimens from the genitourinary tract over a 6-month period revealed 47 (13%) chlamydia-positive cultures, ...197657968
isolation of chlamydia trachomatis in tissue-culture of human thyroid. 197661387
differential response of chlamydial and ureaplasma-associated urethritis to sulphafurazole (sulfisoxazole) and aminocyclitols.91 men with non-gonococcal urethritis (n.g.u.) were randomly treated with either sulphafurazole (sulfisoxazole), 500 mg orally q.i.d. for 10 days, or an aminocyclitol (streptomycin or spectinomycin), 2 g intramuscularity for 1 to 3 doses at 12 h intervals. initial urethral cultures were positive for chlamydia trachomatis (c) in 36 (40%). ureaplasma urealyticum (u) was isolated from the urethra or urine from20 (95%) of 21 white men in a first episode of n.g.u. who had negative chlamydia cultures. ...197663748
[electron microscopic determination of the localization of the group-specific antigen of halprowise (chlamydiae) by the direct immunoperoxidase method].the direct immunoperoxidase technique was applied to the study of the localization of a group-specific antigen of halprowiae (chlamydiae) in the causative agents of meningopneumonia (strain mp) and of paratrachoma (strain lb-i) at the individual stages of their developmental cycle in the l-cell (16, 24 and 48 hours after infection of the culture). the product of reaction to peroxidase pointing to the localization of the group-specific antigen was localized not only on the surface, but in the who ...197666828
chlamydial and ureaplasma-associated urethritis. 197767307
aetiology of acute epididymitis.24 patients with acute epididymitis were examined and underwent urethral and urine cultures for neisseria gonorrhoeae, ureaplasma urealyticum, herpes-simplex virus, chlamydia trachomatis, cytomegalovirus, and gram-negative aerobic bacteria. the results suggest that in young men the sexually transmitted organisms which cause urethritis (n. gonorrhoeae, c. trachomatis, and possibly u. urealyticum) may also lead to acute epididymitis, whereas in older men coliforms and pseudomonas are the predomina ...197767333
[antigenic properties of chlamydia of different origins]. 197876284
photodynamic action of toluidine blue on a chlamydia ornithosis strain. 197880878
antibodies against chlamydia of lymphogranuloma-venereum type in crohn's disease.antibodies against chlamydia of lymphogranuloma-venereum type were detected by the micro-immunofluorescence technique in 38 (69%) of 55 patients with crohn's disease. weak positive reactions were seen in only 2 or 21 patients with other gastrointestinal disorders and 1 of 50 healthy blood-donors.197983465
fulminant psittacosis.two patients died of psittacosis after presenting with generalised toxaemia, acute renal failure, and evidence of pancreatitis. death was attributed to the virulence of the chlamydial strain and the delay in antemortem diagnosis. in one case chlamydia psittaci was isolated from necropsy lung tissue. a third case of psittacosis suggested person-to-person or fomite spread, which is rarely reported. infection was acquired from an apparently healthy, imported and quarantined cockatiel. import restri ...197985004
is trachoma an ocular component of a more generalised chlamydial infection?in industrialised countries, inclusion conjunctivitis of the newborn is now known to be just one manifestation of a more generalised infection with chlamydia trachomatis. pneumonia and enteric infection can develop in perinatally infected infants. it is possible that trachoma in developing countries may reflect extraocular infection that may contribute to the high frequency of pneumonia and diarrhoea in infants in these countries. if true, this would explain the short-term effects of topical tre ...197985939
low frequency of chlamydial antibodies in patients with crohn's disease and ulcerative colitis.serum samples from 55 patients with crohn's disease and from 23 patients with ulcerative colitis were tested for antibodies to chlamydia trachomatis immunotypes by a micro-immunofluorescence technique. antibody titres of 1:8 or greater against several immunotypes were detected in 14.5% of patients with crohn's disease and in 21.7% of those with ulcerative colitis. these figures resemble the incidence in a healthy, non-venereal-disease population. furthermore, there was no correlation between the ...197986884
erythromycin and lymecycline treatment in chlamydia-positive and chlamydia-negative non-gonococcal urethritis--a partner-controlled study.a group of 213 men with non-gonococcal urethritis and their sexual partners were treated either with erythromycin stearate 500 mgx2 for 15 days or with lymecycline 300 mgx2 for 10 or 20 days. chlamydia trachomatis was isolated from 40% of the men, from 26% of their female partners and from 56% of the partners of men with chlamydia-positive urethritis. one hundred and eighty-one men were available for evaluation of therapy. there were no significant differences between the treatment schedules. th ...197987096
chlamydia, cytomegalovirus, and yersinia in inflammatory bowel disease.the relationship of three infectious agents, chlamydia, cytomegalovirus, and yersinia enterocolitica, to the aetiology, clinical course, and diagnosis of crohn's disease was studied. there was no evidence of chlamydial infection in crohn's disease and no indication either that cytomegalovirus alters the outcome of acute attacks or that infection with y. enterocolitica is being misdiagnosed as crohn's disease.197987888
chlamydia and crohn's disease. 197987925
prospective study of chlamydial infection in neonates.chlamydia trachomatis was recovered from the cervices of 4% (36/900) of pregnant women tested. 20 infants born through chlamydia-infected cervices were followed up for a year, as were 18 infants born to chlamydia-negative mothers. a statistically significant excess of conjunctivitis and pneumonia was found in infants exposed to chlamydia. the attack-rate for inclusion conjunctivitis was 35% (7/20) and for chlamydial pneumonia it was 20% (4/20). chlamydiae were recovered from 10 of the 20 (50%) e ...197989446
[detection of humoral antibodies to species-specific and group ornithosis antigens in the dynamics of the infection].the time course of antibody production to group- and species-specific antigens of ornithosis agent was studied by complement fixation (cft) and hemagglutination-inhibition (hi) tests. in rabbits after a single intravenous inoculation of the ornithosis agent, antibody to the homologous species-specific antigen appeared in the peripheral blood 3--5 days after inoculation and reached maximum levels during the 1-st week, and to the group-specific antigen could be detected 3--5 days after inoculation ...197990431
response to treatment of chlamydial infection of uterine cervix. 197990939
preparation of antigens for microimmunofluorescence testing for antichlamydial antibodies. 197990960
direct immunofluorescence tests with counterstains for detection of chlamydia psittaci in infected avian tissues.different methods of preparation and serological evaluation of rabbit globulins for use in fluorescent antibody conjugate and different methods of counterstaining with fluorescent antibody tests were evaluated for detection of chlamydia psittaci in infected turkey tissues. the agar gel precipitin reaction was that chosen for testing and selecting antiserums to be used for fluorescein isothiocyanate conjugation. the fluorescent antibody staining was most pronounced with conjugate made from globul ...197991417
immunofluorescence testing for chlamydial antibodies. 197991741
immunofluorescence testing for chlamydial antibodies. 197991742
optical demonstration of chlamydiae in cell cultures by means of fluorochrome 33,258 h.fluorochrome 33,258 h, a bisbenzimidazole, was employed for demonstrating chlamydiae in cell cultures, and proved to be particularly suitable for illustrating the unique intracellular reproduction processes of these organisms. the staining procedure is simple and permits a selective differentiation of chlamydial inclusions and their developmental processes in the cell. contrary to other staining methods, all stages of the chlamydial reproduction cycle can be illustrated thus permitting the early ...197990017
delayed hypersensitivity to chlamydia trachomatis: cause of chronic prostatitis. 197993221
cytologic investigations in chlamydia infection.chlamydia is an extremely common genital infection. it can cause serious disseminated disease and can infect the neonate and the sexual partner. morphologically, chlamydia infection was identified in routine fast smears obtained from 160 women. chlamydia infection manifests as intracytoplasmic coccoid and inclusion bodies in the metaplastic cells. infected cells also reveal cytomegaly and multinucleation. immunofluorescence studies, electron microscopy, tissue studies, histochemical techniques a ...197993839
chlamydia psittaci: growth characteristics and enumeration of serotypes 1 and 2 in cultured cells.the growth characteristics of chlamydia psittaci serotypes 1 and 2 (ovine and bovine origin) were studed in mouse l cells. formation of inclusions and yield of infectious progeny for serotype 1 were maximal when host cells were treated with cycloheximide and the ph in the cell culture medium was 7.2-7.4. the number of cells that contained inclusions and the infectivity yield for serotype 2 were maximal when the ph was 6.6-7.0. treatments with diethylaminoethyl dextran and cycloheximide increased ...197994339
[comparative study of various chlamydia strains--preliminary study (author's transl)]. 197994564
current concepts in ophthalmology. ocular infections. 197896339
microbiology of the vagina in children: normal and potentially pathogenic organisms.vaginal cultures from 100 healthy girls, 2 months to 15 years of age, were examined for the presence of normal and potentially pathogenic microorganisms. corynebacterium vaginale, yeast species, and genital mycoplasmas were isolated from vaginal cultures from 13.5 %, 28%, and 28% of the girls examined, respectively. colonization with these organisms was not associated with signs or symptoms of vaginitis. neisseria gonorrhoeae was isolated from a 4-year-old with purulent vaginitis. trichomonas va ...197898750
[isolation and identification of clamydia psittaci as the pathogen in enzootic abortion of sheep in eastern slovakia].abortions in ewes occurred on a large scale in three localities in eastern slovakia. antibodies to the group-type ornithosis antigen were detected in titres of 1 : 128 to 1 : 2048 in the aborting ewes in the mentioned localities. suspensions were prepared from the four samples of material, obtained either from the placentae and afterbirths of the aborting ewes or from the tissues of the aborted foetuses. seven-day old yolk sacs of chicken embryos were infected with these suspensions. four strain ...197899861
comparison of direct and modified direct complement-fixation and agar-gel precipitin methods in detecting chlamydial antibody in wild birds.detection of chlamydial antibody in serums of wild birds was compared for the following methods: direct complement-fixation (dcf), modified dcf (mdcf), and agar-gel precipitin (agp). the birds species used were great-tailed grackles (cassidix mexicanus), common grackles (quiscalus quiscula), brown-headed cowbirds (molothrus ater), bronzed cowbirds (tangavius aeneus), and mourning doves (zenaida macroura). the birds were either inoculated with an isolate of chlamydia psittaci obtained originally ...1978100098
isolation of a type-specific antigen from chlamydia trachomatis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.this report describes the isolation of a type-specific antigen of a serotype a strain of chlamydia trachomatis. the antigen could be identified in sodium dodecyl sulfate-polyacrylamide electrophoretic (sds-page) analysis of immunoprecipitates of homologously reacted lysates from bolton-hunter 125i-labeled elementary bodies, solubilized by sonication and treatment with nonidet p40. the electrophoretic pattern of this precipitate revealed a peak of unique mobility that was not reproduced by hetero ...197984016
a comparison of genital infections caused by chlamydia trachomatis and by neisseria gonorrhoeae.patients with culture-proven infections with neisseria gonorrhoeae or chlamydia trachomatis, or both, seen at the olmsted county health department sexually transmitted disease clinic were evaluated for the presence of dysuria and exudate. eighty-four patients (21%) had neither symptom. n. gonorrhoeae infection alone was most frequent (47%), but c. trachomatis predominated in men. dysuria was most often detected in men infected with c. trachomatis, but exudate was most frequently found in men inf ...1978101076
viral infections and ige levels.six non-atopic patients with respiratory infections to a variety of viruses demonstrated a consistent drop in ige levels (35% to 87%) in the convalescent phase compared to the acute phase of infection, suggesting that the viral agent affects t lymphocytes to suppress and hence b lymphocytes to secrete ige immunoglobulin.197880144
[acute urethritis--an overview]. 1978101824
[unspecific chronic "prostato-urethritis"]. 1978101828
[the value of the urethral smear for the diagnosis of unspecific prostato-urethritis]. 1978101831
[therapy of prostato-vesiculitis]. 1978101836
separation and partial characterization of a type-specific antigen from chlamydia trachomatis.type-specific antigens of chlamydia trachomatis have been demonstrated by the mouse toxicity prevention test and a variety of immunofluorescent techniques. in addition, biologic activity has been associated with these antigens in terms of type-specific immunity to trachoma infections. this report is the first to describe the detection of a soluble type-specific antigen of c. trachomatis and its separation from those antigens that cross-react among different immunotypes. test antigens were prepar ...197878941
chlamydia trachomatis urethritis in men attending a venereal disease clinic: a culture and therapeutic study.urethral specimens from 459 male patients attending a venereal disease clinic were studied for presence of chlamydia trachomatis and neisseria gonorrhoeae. of the 459 patients, 362 had symptoms suggestive of urethritis. in these 362, gonorrhoea was diagnosed in 78 (22%), while 88 (24%) harboured c. trachomatis; 15 harboured both organisms. of those patients from whom c. trachomatis was isolated, 17% had no subjective symptoms of urethritis; the same percentage of symptomless carriers of gonococc ...197876400
morphological and cytochemical study of chlamydia with edta regressive technique and gautier staining in ultrathin frozen sections of infected cell cultures: a comparison with embedded material.the cryo-ultramicrotomy technique was applied to study the ultrastructure of chlamydia using two strains: one of c. psittaci and one of c. trachomatis. it clearly appeared that in both strains reticulate bodies show a high degree of plasticity, contrasting with the rigid spherical appearance of elementary bodies. ultrastructural cytochemical study shows dna fibrils dispersed throughout the cytoplasm in reticulate bodies whereas dna is condensed in a nucleoid in elementary and intermediate bodies ...1978106752
[the isolation of chlamydia in the male urogenital tract]. 1979107412
antichlamydial antibody in genital exudates of men and women with non-gonococcal genital infections. 197876057
experimental nasopharyngitis and pneumonia caused by chlamydia trachomatis in infant baboons: histopathologic comparison with a case in a human infant.three infant male baboons were inoculated with a strain of chlamydia trachomatis isolated from a human infant with pneumonitis. one baboon, inoculated by intratracheal, nasopharyngeal, and oropharyngeal seeding, had rales, radiographic evidence of pneumonia, persistent nasopharyngeal c. trachomatis infection, and a four-fold rise in titer of antibody. at sacrifice 24 days after inoculation, nasopharynx, trachea, airways, and lung yielded c. trachomatis, and epithelial inclusions were seen by lig ...1979108341
treatment of chlamydia trachomatis infections. 197874613
ansamycins. chemistry, biosynthesis and biological activity. 197774108
[schiff's test in diagnosis of ophthalmogalprovioses (chlamydioses)]. 197774054
chlamydia trachomatis infection and veneral disease.chlamydia trachomatis was isolated by the irradiated mccoy cell technique from 44 out of 103 men with non-gonorrhoeic urethritis and from 11 out of 15 patients with post-gonococcal urethritis. in women attending the venereal diseases clinics, chlamydial infection was observed in 49 out of 130 patients (38%), an infection incidence of the same order of magnitude as the one noted for gonococcal infection (40%). in 19% both infections occurred simultaneously. treatment with tetracycline eliminated ...197773314
enzyme-linked immunosorbent assay for chlamydial antibodies.an enzyme-linked immunosorbent assay (elisa) detected chlamydial antibodies in human sera. the assay antigen produced in cell cultures infected with chlamydia psittaci was formalin-fixed to microplates. single convalescent-phase sera positive for chlamydial antibodies by a complement-fixation test were positive at even higher dilutions by elisa. paired sera with diagnostic rises in complement-fixing antibody showed seroconversion by elisa also. control sera from persons with no history of chlamy ...197772759
chlamydial infections of the eye. 197772200
early detection of chlamydial inclusions combining the use of cycloheximide-treated mccoy cells and immunofluorescence staining.detection of chlamydia trachomatis inclusions only 21 h after a specimen reaches the laboratory has been achieved by the combined use of cycloheximide-treated mccoy cells and immunofluorescence staining. moreover, cells exposed to cycloheximide were more sensitive for detecting chlamydial inclusions than those pretreated by irradiation, since larger numbers of inclusions were found in the former cells. the application of this rapid and sensitive method allows a diagnosis of chlamydial infection ...197771306
[detection of chlamydia by the immunofluorescence method].the possibility of detecting chlamydia by the direct immunofluorescence method was tested in the impression preparation of experimentally infected mice, chick embryos and naturally infected domestic mammals. in comparison with slight-microscope detection, this method was found to be sensitive and expedient. the greatest amount of antigen could be detected in the lungs of mice after intranasal infection already within 6 to 12 hours. in the other organs the findings were less ample; the visualizat ...1979117591
chlamydia trachomatis and the respiratory tract. 197766577
purification of a chlamydia trachomatis-specific antigen by immunoadsorption with monospecific antibody.this study describes the isolation and partial characterization of a chlamydia trachomatis specific antigen. a species-specific antigen of c. trachomatis (antigen-0.65) was identified by two-dimensional immunoelectrophoresis. antiserum specific for antigen-0.65 was prepared in rabbits by immunizing with agarose-gel precipitates excised from two-dimensional immunoelectrophorograms. purified gamma-globulins from antigen-0.65 specific serum were coupled to the n-hydroxysuccinimide ester derivative ...197765424
[genital infections by viruses, mycoplasma and chlamydozoa (author's transl)]. 197661630
letter: growth of chlamydia trachomatis in cell culture. 197656688
ultrastructural studies of the nucleoids of the pleomorphic forms of chlamydia psittaci 6bc: a comparison with bacteria.the nucleoids of the various pleomorphic forms of chlamydia psittaci have been examined by direct observation of infected cells and by observations on isolated particles. the fixation and staining methods used were the same as those routinely used for the examination of bacteria to facilitate the comparison of chlamydial fine structure with that of bacteria. the nucleoids of reticulate bodies were composed of fine fibrils which extended throughout these particles. the nucleoids of intermediate b ...197656212
some properties of the polysaccharide from cell cultures infected with tric agent (chlamydia trachomatis).the polysaccharide, elaborated in trachoma-inclusion conjunctivitis (tric) agent inclusions, was isolated from baby hamster kidney (bhk) cells grown and infected in suspension cultures. it was characterized by physical, chemical and enzymic methods as a glycogen with an average chain length of 14 to 16 glucose units.197551905
a search for mycoplasmas and chlamydiae in acne lesions.no mycoplasmas and no chlamydiae were found in cultures of 75 uninflamed comedones, 72 papulopustular lesions, and 7 cystic lesions from 14 subjects with acne. chlamydial serologic tests were negative for 11 subjects and showed antibodies in 3 subjects to 3 different antigenic types of trachoma agent. these results are viewed as substantial evidence against the possibility that either mycoplasmas or chlamydiae play an important part in the pathogenesis of acne.1976133192
spectinomycin hydrochloride in the treatment of gonorrhoea: its effect on associated chlamydia trachomatis infections.sixty-three heterosexual men were successfully treated with a single injection of spectinomycin hydrochloride 2 g for urethral infections with neisseria gonorrhoeae. chlamydia trachomatis was recovered from the urethra of 11 of these men both before and after treatment. in six men, the organism was isolated after but not before treatment. no isolates were obtained from the remaining men either before or after treatment. all 17 of the men who yielded c. trachomatis developed post-gonococcal ureth ...1977144543
[epidemiologic study data on foci of enzootic abortion of sheep in rostov province].epidemiological and serological investigations of diseases among cattle-breeders aimed at the elucidation of the etiological importance of mammalian chlamydia in human pathology were carried out in cattle-breeding farms where chlamydial infection in sheep (enzootic abortion of sheep) had been diagnosed previously. in these foci with the suppressed enzootic process from 6.2% to 15.6% of the personnel attending to the animals were found to be infected. retrospectively, patients and convalescents w ...1977145094
tetracyclines: new look at old antibiotic. ii. clinical use. 1978149261
cytology as a guide to the presence of chlamydial inclusions in giemsa-stained conjunctival smears in severe endemic trachoma.microscopical examination of 927 giemsa-stained conjunctival smears from children with chronic trachoma in southern tunisia showed 93 (10 per cent.) with typical trachoma (chlamydial) inclusions in epithelial cells. the accompanying cytological features were a useful indicator for inclusions. inclusions were found only in slides with numerous polymorphonuclear neutrophils (pmns) and separation of the epithelial cells. when these two features alone were present, 3 per cent. of the smears were inc ...197548384
chlamydia trachomatis in gonococcal and postgonococcal urethritis.chlamydia trachomatis was isolated from the urethra of 38 (28.6%) out of 133 men with gonococcal urethritis (gu). during the follow up of 72 men postgonococcal urethritis (pgu) was diagnosed in 50 (69.5%) patients. more than half (30 out of 50) of these patients with pgu were chlamydia-positive. out of 31 patients with chlamydia 30 developed pgu whether or not procaine penicillin, spectinomycin, or gentamicin were used. these findings are discussed in relation to present recommendations for the ...1978152142
ankylosing spondylitis and chlamydial infection in apparently healthy hla b27 blood donors. 1979160457
isoelectric focusing of chlamydia trachomatis.hela-229 cells and the elementary bodies of chlamydia trachomatis had a net negative electrical surface charge at neutral ph when measured by isoelectric focusing. inclusion-forming and non-inclusion-forming elementary bodies focused in one band at pi 4.64.197944706
acute perihepatitis.fourteen young females with acute pains in the right hypochondrium were admitted to the surgical department. the history and clinical findings were typical of acute perihepatitis (fitz-hugh-curtis syndrome), and the diagnosis was confirmed by laparoscopy. the gynecological symptoms and signs were negligible, and neisseria gonorrhoeae were cultured from the endocervix in two of the patients. pathogenes in the suprahepatic space were not demonstrated. eight patients used intrauterine contraceptive ...1979161459
the cell as an extreme environment.living cells and their intracellular parasites show many of the characteristics ascribed to extreme environments and their dominant species. the diversity of species colonizing intracellular habitats is low, and successful inhabitants exhibit special fitness traits that often render them obligately dependent on residence within a host cell. however, the diversity-limiting factor in the extreme environment of the host cell interior is not abiotic, as it is in conventional extreme environments. it ...197936622
protein-carbohydrate-lipid complex isolated from the cell envelopes of chlamydia psittaci in alkaline buffer and ethylenediaminetetraacetate.exposure of isolated cell envelopes from purified infectious elementary (eb) of chlamydia psittaci to sodium carbonate-bicarbonate buffer at ph 10 plus ethylenediaminetetraacetate (edta) results in partial solubilization of the total protein. the released materials represent 20% of the dry weight, 16% of the total protein, 40% of the total carbohydrate, and 9% of the total lipid of the cell envelopes. sucrose density gradient centrifugation, and sephadex g-200, sepharose 4b, or diethylaminoethyl ...19761376
effect of alkali on the structure of cell envelopes of chlamydia psittaci elementary bodies.suspensions of isolated cell envelopes of infectious elementary bodies (eb) of chlamydia psittaci at alkaline ph showed a rapid, extensive decrease in absorbance, accompanied by the release of a cell envelope component in a sedimentable form. this phenomenon was observed both at 0 c and with envelopes which had been previously heated to 100 c. monovalent and divalent cations effectively inhibited the turbidity loss, whereas ethylenediaminetetraacetate (edta) caused an accelerated decrease in tur ...19761375
early detection of chlamydia trachomatis using fluorescent, dna binding dyes.hela 229 cells were infected with genital tract strains of chlamydia trachomatis. after incubation for varying times the infected cells were fixed and stained with the fluorescent dna binding dyes hoechst 33258 or dapi for comparison with conventional giemsa stain. fluorochrome-treated preparations were examined by incident ultraviolet fluorescence microscopy and the giemsa-stained preparations by dark-ground light microscopy. chlamydial inclusion bodies could be identified unambiguously as earl ...197992480
chlamydia trachomatis: a cause of an infantile pneumonia syndrome. 1978101061
cell-mediated immune responses in owl monkeys (aotus trivirgatus) with trachoma to soluble antigens of chlamydia trachomatis.the first temporal study of the cell-mediated immune responses (cmi) following ocular infections with chlamydia trachomatis is presented. we examined the cmi of owl monkeys infected with trachoma to soluble antigens of c. trachomatis by leucocyte migration inhibition (lif) and delayed hypersensitivity skin testing. delayed hypersensitivity of a systemic nature developed after a local eye infection in owl monkeys; clearance of inclusions from conjunctival cells coincided with the onset of this re ...1978101327
studies of the viral flora in keratoconjunctivitis sicca.fifty patients suffering from kcs and exhibiting various patterns of corneal staining with fluorescein were examined for adenovirus types 3, 7, 8, and 14, herpes simplex, and the tric group by tissue culture and immunofluorescein techniques. no viruses were detected by either methods, and it is considered that presumption of virus involvement in these cases is untenable.1975164882
etiology of nongonococcal urethritis.chlamydia trachomatis was isolated from the urethra from 48 (42 per cent) of 113 men with non-gonococcal urethritis (ngu), four (7 per cent) of 58 without overt urethritis, and 13 (19 per cent) of 69 with gonorrhea. postgonococcal urethritis (pgu) developed in 11 of 11 men who had c. trum antibody to c. trachomatisis developed. the immunotype specificity of chlamydial antibody corresponded to the immunotype isolated. among culture-negative patients. chlamydial antibody prevalence correlated with ...1975165407
editorial: etiology of nongonococcal urethritis one piece in the puzzle. 1975165408
a serologic survey of pronghorns in alberta and saskatchewan, 1970-1972.to determine the exposure of free-ranging pronghorns (antilocapra americana ord) to selected pathogens, serum samples were obtained from 33 live-trapped animals from southwestern saskatchewan in 1970, and from 26 and 51 animals from southeastern alberta, in 1971 and 1972, respectively. antibodies were found to the agents of parainfluenza 3, bovine virus diarrhea, eastern and western encephalomyelitis, infectious bovine rhinotracheitis and the chlamydial group. no serologic reactors were found to ...1975167203
streptovirudins, new antibiotics with antibacterial and antiviral activity. i. culture taxonomy, fermentation and production of streptovirudin complex.a new antibiotic complex has been isolated from cultures of streptomyces strain no. ja 10124. on the basis of taxonomic studies, the producing microorganism is described as streptomyces griseoflavus (krainsky, 1914) waksman et henrici, 1948, subsp. thuringiensis subsp. nov., type strain ja 10124. the antibiotic complex, designated as streptovirudin, was isolated from extracts of both mycelium and culture filtrate. it is a white amorphous material which consists of ten closely related components ...1975168173
importance of the so-called 'other' sexually-transmitted diseases.(1) some data are presented concerning the frequency and potential morbidity of sexually-transmitted organisms other than t. pallidum or n. gonorrhoeae. (2) most of the diseases with which these organisms are associated are more prevalent than syphilis and some, at least in one sex, are as common as gonorrhoea. a number appear to carry considerable morbidity, which in the case of type ii herpes virus--if it is responsible for cervical cancer--may ultimately cause more fatalities than syphilis. ( ...1975168937
[morphological manifestations of the intracellular toxic effect of microorganisms of the ornithosis-lymphogranuloma-trachoma group].interactions of the causative agents of meningopneumonia and ovine abortion with pulmonary macrophages in experimental infection in white mice were studied by histological, immunofluorescent and electron microscopic methods. the causative agent of meningopneumonia did not multiply in colonies and caused toxic lesions in the cells and formed cytoplasmic oxiphilic inclusion bodies. being the product of cell degeneration, these oxiphilic inclusion bodies were conducive to long-term survival of the ...1975169631
nonbacterial respiratory infections. 1975171127
chlamydia trachomatis cervicitis in gynecologic outpatients.symptoms suggestive of a lower genital tract infection (lgti) are common complaints in women who consult gynecologists. sexually transmitted microorganisms, such as chlamydia trachomatis. neisseria gonorrhoeae, and trichomonas vaginalis, are responsible for a substantial proportion of lgti. this study was performed to establish the frequency of lgti caused by c trachomatis in women attending a gynecologic outpatient clinic. of 170 women with lgti, 32.9% harbored one or more of these organisms: c ...1978104212
search for uro-genital tract infections in patients with symptoms of prostatitis. studies on aerobic and strictly anaerobic bacteria, mycoplasmas, fungi, trichomonads and viruses.seventy-nine patients with symptoms of nonacute prostatitis and 20 healthy volunteers were examined for uro-genital tract infection with bacteria, mycoplasmas, fungi, trichomonads and viruses. no differences in the results of the bacterial cultures were found between the patients and the controls. in only a few cases were established urinary tract pathogens found, but in no instance were these findings reproducible in later specimens. the cultures of the expressed prostatic fluids and the sample ...1975175434
[combined vaccination of sheep against anthrax, pox and anaerobic infections]. 1975175545
trachoma in a rural haitian community.we selected 43 patients, aged 15 days to 57 yesrs, for intensive study after screening 975 people for clinical evidence of trachoma in rural haiti. trachoma was present in haiti with low endemicity and was followed by relatively mild sequelae. laboratory studies confirmed the disease through demonstration of conjunctival inclusion bodies by immunofluoresence and serotyping of antibody in sera or eye secretions, or both. of 23 patients (age 1 to 36 years) with active trachoma, all had serum antib ...1976175658
feline respiratory infections--a clinical review. 1976177166
isolation of chlamydia trachomatis from bartholin's ducts.exudate from bartholin's ducts from 30 selected patients was investigated for chlamydia trachomatis and neisseria gonorrhoeae. n. gonorrhoeae was isolated from the duct exudate in 24 patients and c. trachomatis in nine. concurrent infection of the ducts was present in seven (29.2%); the remaining two patients were sexual contacts of men with non-specific urethritis. the duct exudate was mucopus in seven patients, cloudy mucus in one, and clear mucus in the other. although contamination of the vu ...1978104771
experimental acute salpingitis in grivet monkeys provoked by chlamydia trachomatis.chlamydia trachomatis is a common cause of sexually transmitted diseases. recently it has been shown that chlamydiae are also responsible for complications to such lower genital tract infections. in this study, isolates of c. trachomatis from the fallopian tubes of patients with acute salpingitis were inoculated direct into the fallopian tubes of two, and through the cervical canal into the uterine cavity of one grivet monkey. the experimental infections resulted in a self-limited acute salpingi ...1979107723
chlamydia trachomatis in acute salpingitis.in a study to evaluate the possible role of chlamydia trachomatis and neisseria gonorrhoeae in acute salpingitis, 26% of 106 patients with severe symptoms had positive culture results for c. trachomatis; 43% of the 72 patients from whom paired sera were obtained had either positive culture results for or seroconversion in the single antigen immunofluorescence test to c. trachomatis. twenty-six per cent of patients harboured n. gohorrhoeae and 14% had gonococcal complement-fixing antibody titres ...1979111767
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