| [immunofluorescent test of rickettsia microagglutination. 3. isolation of antibodies to rickettsia quintana]. | | 1976 | 62095 |
| a passive hemagglutination test for diagnosis of trench fever due to rochalimaea quintana. | a passive hemagglutination test devised for diagnosis of trench fever was easily performed and highly sensitive and specific. tanned sheep erythrocytes were sensitized with soluble antigen from rochalimaea quintana. the test detected antibody in six of seven cases of primary infection and in four cases of late, relapsed trench fever. titers of antibody ranged from 1:20 to 1:640. although both igm and igg antibody to r. quintana were detected by passive hemagglutination, igg appeared to be the ma ... | 1976 | 63526 |
| [formamidase--microheterogeneity, catalytic properties and inhibitors (author's transl)]. | formamidase from rat liver proved to be microheterogenous. after preparative isoelectric focusing in density gradient columns, two peaks of formamidase with identical substrate specificity were identified. by analytical focusing in thin layers of polyacrylamide or sephadex g-75 sf, even five bands could be separated. their isoelectric points were 4.75, 4.78, 4.82, 4.92 (main band) and 5.11, but their michaelis constants did not differ significantly (54 to 62 mumol/l). an identical molecular weig ... | 1979 | 84781 |
| chlorpyrifos and 3,5,6-trichloro-2-pyridinol: residues in the body tissues of cattle wearing chlorpyrifos-impregnated plastic ear bands. | | 1978 | 84823 |
| safety of hepatitis-b vaccines containing intact 20 nm particles. | | 1979 | 85121 |
| [role of anti-brain antibodies in the development of mineralocorticoid deficiency]. | | 1979 | 85292 |
| [relationship of the structure of specific somatic antigen polysaccharides in shigella dysenteriae bacteriae to the immunochemical properties]. | | 1979 | 85516 |
| preganglionic neurons in the sacral spinal cord of the rat: an hrp study. | | 1979 | 86176 |
| definition of two ld antigens in rhesus monkeys. | two rhesus (macaca mulatta) monkey lymphocyte-defined (ld) antigens have been identified using two typing cells as stiumlators in a one-way mixed leukocyte culture (mlc) assay. an analysis of the genetic behavior of these ld antigens in six rhesus monkey families revealed that both antigens were linked with rhla. one probable recombinant indicated that the ld locus lies outside the two known rhla-sd loci and the locus which controls the serum protein, properdin b(bf). these two antigens, ld1 and ... | 1979 | 87026 |
| potentiating effect of nitrate and other anions on contraction in vascular smooth muscle. | | 1978 | 87173 |
| anaphylatoxin-induced shock and two patterns of anaphylactic shock: hemodynamics and mediators. | in the dog, different cardiorespiratory reactions were identified in two types of anaphylactic shock and in c5a-at (anaphylatoxin)-induced shock. all three types had in common a portal blood pooling with consequent decrease in the venous return, cardiac output, and arterial pressure. in anaphylaxis (a) of the first type, at a low titer of hemagglutinating antibodies, the latent period was 68 s and heart and lung function was unchanged. in the second type, at high titer, the latency was 19 s and ... | 1979 | 88165 |
| immunologic studies of native and modified human factor viii/von willebrand factor. | factor viii/von willebrand factor (fviii/vwf) is a glycoprotein with a molecular weight greater than one-million daltons. two activities are associated with this large molecule: fviii procoagulant activity and vwf activity. incubation of fviii/vwf with proteolytic enzymes causes rapid inactivation of the fviii procoagulant activity but has little effect on the vwf activity or antigenicity. in an attempt to gain insight into the structural features required for these two activities, antisera were ... | 1979 | 88237 |
| hippocampal evoked potentials and eeg changes during classical conditioning in the rat. | hippocampal evoked potentials (eps) and eeg responses were studied in rats, using a classical conditioning paradigm (water, us), with a spatially discontiguous cs-us arrangement in order to separate the cs and goal-related responses. in early training, when the orienting score was high, the tone cs, instead of eliciting a definite ep, usually reset hippocampal theta activity in phase, i.e. theta rhythm became time-locked to cs. with further training, orienting activity (ori) decreased to the pre ... | 1979 | 88362 |
| systemic hyalinosis (juvenile hyaline fibromatosis). ultrastructure of the hyaline with particular reference to the cross-banded structure. | systematic hyalinosis (juvenile hyaline fibromatosis) is characterized by hyalinized skin lesions. electron microscopic examination of a hyalinized skin tumor from a 19-year-old man with this syndrome revealed that the hyaline was composed of ruthenium red-positive ultrastructures (granules, filaments, and a kind of cross-banded structure), indicating the presence of glycosamino-glycan or glycoprotein, and a small number of thin collagen fibrils. using a new ruthenium red staining method combine ... | 1979 | 88923 |
| prolonged survival of corneal allografts in rabbits treated with cyclosporin a. | | 1979 | 90771 |
| [antibody response to hemagglutinin and neuraminidase induced by an influenza subunit vaccine (author's transl)]. | to study antigenicity, persistence of antibody (efficacy) and reactogenicity of a new bivalent influenza subunit vaccine, sandovac, it was given in two different concentrations (1000 and 2000 iu) to 156 subjects from two epidemiologically destinct areas (119 students from essen, average age 22 years, 37 residents from kassel average age 56 years). serum antibody response was measured by hemagglutination inhibition (hi) and neuraminidase inhibition (ni) tests before, one, two and six months after ... | 1979 | 92113 |
| quantitative aspects of the naphthol yellow s staining for proteins studied in a model system of polyacrylamide films and in isolated rat liver cells and nuclei. | after staining with naphthol yellow s (nys) at optimal conditions of ph (2.8), the protein content of rat liver cells isolated by means of a collagenase perfusion technique was found to be cytophotometrically immeasurable, because of too high local dye absorbances. in order to lower the absorption values, techniques to flatten the cells, off-peak measurements and nys staining at non-optimal ph levels were applied. with polyacrylamide model films incorporated with albumin, the reliability of off- ... | 1979 | 95604 |
| deficiency of the ninth component of complement in man. | the studies of serum from a case with c9 (the ninth component of complement) deficiency are described. a 29-year-old woman in good health was found to have low serum complement levels (ch50). c9 of her serum was undetectable by the hemolytic assay and by the immunochemical analysis but all other components were normal. it was demonstrated that low ch50 of her serum was due to the hemolysis of the sensitized sheep erythrocytes (ea) by the complement components from c1 to c8. | 1979 | 95812 |
| [the lice of the gerbils of tajikistan]. | 2606 gerbils of four species from tajikistan were examined. on them there were found over 14 thousand sucking lice of 10 species, four of which are typical to gerbils. hoplopleura merionidis ferris is a mongolian-turkestan species, which in central asia parasitizes meriones meridianus pallas and m. unguiculatus milne-edw. and in middle asia--m. meridianus and m. erythrourus gray, seldom m. tamariscinus pallas. polyplax chinensis ferris, a parasite of m. meridianus, is a mongolian-turkestan speci ... | 1979 | 95816 |
| [the adrenergic innervation of the thoracic lymphatic duct in dogs in postnatal ontogeny]. | using histochemical fluorescence technique, studies have been made on the adrenergic innervation of the wall of the thoracal lymphatic duct in postnatal development of puppies. adrenergic fibers were found in the adventitial layer of the duct. during postnatal development of the animals, adrenergic innervation of this duct becomes more perfect, reaching the level of adult animals to the age of 2-3 months. | 1979 | 95857 |
| naturally occurring diatomaceous pneumoconiosis in sub-human primates. | | 1978 | 96157 |
| [3h]serotonin release: an improved method to measure mast cell degranulation. | a method based on the release of tritium-labelled serotonin by activated mast cells in rodents is described. mast cells incorporate labelled serotonin selectively and release the label after activation by non-specific stimulators (compound 48/80, polymyxin b sulphate, atp, bovine chymotrypsin and l-alpha-lysophosphatidylcholine) or anaphylactic antibody and the corresponding antigen. these two types of activation were investigated in comparison with the toluidine blue microscopic rat mast cell d ... | 1978 | 96185 |
| the fetus, pregnancy and parturition. | | 1977 | 98095 |
| aerosolized antigen dose-response studies in asthmatic monkeys. | this study used rhesus monkeys which had consistent respiratory responses to a standard (single dose) aerosolized concentration of a. beginning at lower concentrations of a which did not produce an airway response, fourfold increases in concentrations of a were aerosolized until an airway response occurred. compared with the single-dose sac after the tac, some consistently reactive animals did not respond to the sac, the maximal concentration of a used for testing. thus the tac may result in an ... | 1978 | 98604 |
| immunochemical analysis of the conformational properties of intermediates trapped in the folding and unfolding of bovine pancreatic trypsin inhibitor. | | 1978 | 98640 |
| differential tolerance to pentobarbital in rats bred for differences in alcohol sensitivity. | two lines of rats bred for differences in motor impairment following alcohol treatment were also found to be differentially affected by sodium pentobarbital in three experiments. the most affected (ma) animals, bred for sensitivity to alcohol, showed a decrement in stabilimeter activity at doses of 8 mg and 16 mg pentobarbital per kg body weight. the least affected (la) animals, bred for insensivity to alcohol, were affected only by the higher dose, at which the resulting impairment was still le ... | 1978 | 98785 |
| peptide hydrolases of lactobacillus casei: isolation and general properties of various peptidase activities. | discovery of an endopeptidase by gel chromatography and separation of 3 exopeptidases (a dipeptidase, an aminopeptidase and a specific carboxypeptidase) from lactobacillus casei ncdo 151 by affinity chromatography is described. the 3 exopeptidases were strongly inhibited by the metal chelators edta and 1,10-phenanthroline but were reactivated with co2+ and mn2+. the ph optima for aminopeptidase, dipeptidase and carboxypeptidase activities were 6.5, 7.6 and 7.2, respectively. maximum activity was ... | 1978 | 101561 |
| congenital defects due to hyperthermia. | | 1978 | 104560 |
| is gip a glucagon cell constituent? | "gastric inhibitory peptide" or "glucose-dependent insulin-releasing peptide" (gip) is a member of the gut hormone family. its physiological action is thought to be related to its insulinotrophic effect. the occurrence and distribution of gip was studied by immunohistochemistry. in all species examined including man, gip immunoreactivity was found to reside in the glucagon cells of the pancrease and gut. three pancreatic glucagonomas were found to contain numerous cells displaying gip and glucag ... | 1978 | 104936 |
| rapid, sensitive and specific electron capture-gas chromatographic method for the quantitation of 6-chloro-2-(1-piperazinyl) pyrazine in biological fluids. | a highly specific and sensitive gas chromatographic method for the determination of 6-chloro-2-(1-piperazinyl)pyrazine (mk-212), a central serotonin-like agent, in biological fluids is described. mk-212 and a related internal standard are extracted into benzene from an alkaline solution, back-extracted into acid and then re-extracted into benzene at an alkaline ph. the amines are converted to the trifluoroacetyl derivatives (characterized by gas-liquid chromatography-mass spectrometry), chromato ... | 1979 | 105011 |
| effect of specific antisera on the redistribution of chlorinated biphenyls in the mouse. | | 1979 | 105217 |
| induction of reaginic (ige) gonococcal antibodies in the rat by a common antigen of neisseria gonorrhoeae. | an antigen (zab) common to neisseria gonorrhoeae was prepared by stepwise elution of a crude gonococcal antigen (za) from columns of diethylaminoethyl cellulose employing 0.02 m phosphate buffers, ph 7.6, containing increasing concentrations of sodium chloride. rats immunized with zab produced reaginic (ige) antibody which cross-reacted with za prepared from eight gonococcal strains by the passive cutaneous anaphylaxis (pca) test. heating of the sera at 56 degrees c for 4 h destroyed the pca act ... | 1979 | 108009 |
| galvanized caging as a potential factor in the development of the "fading infant" or "white monkey" syndrome. | four rhesus females and their infants were kept in galvanized enclosures, and three were kept in stainless steel caging. all four offspring in the galvanized enclosures developed achromotrichia, alopecia, and weakness that varied from modererate to severe while the three infants in stainless steel cages were clinically normal. plasma copper, zinc, and iron values as well as liver copper and zinc values of the infants were compared for the two types of caging. plasma copper values were significan ... | 1978 | 108464 |
| comparison of 15n flux and nitrogen balance with carcass analysis during oral and iv nutrition. | | 1979 | 120030 |
| [heterogeneous and kinetic analysis of certain properties of ca2+-atpase in sarcolemma of rabbit skeletal muscles]. | kinetic properties of ca2+-atpase were studied by means of the continuous potentiometric method. on the basis of heterogenous and kinetic ideas it is shown that the experimental data on the effect of ions ca2+ on the ca2+-atpase activity may be explained, assuming the chelate complex ca-atp2- to be a substrate of the enzyme. the results obtained show that if the chelate complex formed in the aqueous stage is a substrate, then, a competition for adsorption at the active centre is possible between ... | 1975 | 128175 |
| [morphological and biochemical studies on the retinal blood vessels of streptozotocin diabetic rats (author's transl)]. | | 1975 | 130794 |
| developmental patterns of peripheral nerve, myoneural junction and muscle: a review. | | 1977 | 148071 |
| radioimmunoassay for the detection and quantitation of 5-fluorodexoyuridine. | a sensitive radioimmunoassay for 5-fluorodeoxyuridine (fdurd) and 5-fluoro-2'-deoxyuridine 5'-monophosphate has been developed by using antibody induced in rabbits, [3h]fdurd, and a separation technique with nitrocellulose filters. antibody specificity was characterized by using compounds chemically related to fdurd and comparing their effectiveness for displacing [3h]fdurd from the antibody-combining site. drug levels in serum samples from patients receiving fdurd chemotherapy were easily and r ... | 1978 | 148966 |
| divergent actions of protein kinase modulator in regulating mammalian cyclic gmp-dependent and cyclic amp-dependent protein kinases. | protein kinase modulator can either augment or depress phosphorylation of substrate proteins catalyzed by cyclic gmp-dependent and cyclic amp-dependent protein kinases prepared from mammalian and arthropod tissues. alteration by the modulator of the phosphorylating activity of the protein kinases is considered to be due to modification of protein substrate specificity subsequent to interaction of the modulator with the catalytic subunits of the enzymes. it is likely that the physiologic role of ... | 1975 | 165354 |
| adenosine 3':5'-cyclic monophosphate phosphodiesterase activity in the dystrophic rat retina. | | 1975 | 165991 |
| restoration of ultraviolet-induced unscheduled dna synthesis of xeroderma pigmentosum cells by the concomitant treatment with bacteriophage t4 endonuclease v and hvj (sendai virus). | ultraviolet (uv)-induced unscheduled dna synthesis of xeroderma pigmentosum cells, belonging to complementation groups a, b, c, d, and e, was restored to the normal level by concomitant treatment of the cells with t4 endonuclease v and uv-inactivated hvj (sendai virus). the present results suggest that (1) t4 endonuclease molecules were inserted effectively into the cells by the interaction of hvj with the cell membranes, (2) the enzyme was functional on human chromosomal dna which had been dama ... | 1975 | 172893 |
| [inhibitory effect of low temperature on collagenase activity in superficial experimental corneal wounds (author's transl)]. | | 1979 | 228110 |
| structure and specific sequences of avian erythroblastosis virus rna: evidence for multiple classes of transforming genes among avian tumor viruses. | two major rna species were found in several clonal isolates of avian erythroblastosis virus (aev) and avian erythroblastosis-associated helper virus (aeav) complexes: one of 8.7 kilobases (kb), the other of 5.5 kb. the 5.5-kb species was identified as aev rna because (i) it was absent from non-transforming aeav isolated from the same virus complex, (ii) it was present in complexes of aev and different helper viruses, and (iii) its structure is similar to that of avian acute leukemia viruses of t ... | 1979 | 228273 |
| is the mitochondrially made subunit 2 of cytochrome oxidase synthesized as a precursor in neurospora crassa? | | 1979 | 228973 |
| a small, unstable rna molecule of escherichia coli: spot 42 rna. ii. accumulation and distribution. | | 1979 | 229230 |
| the replication of subgroup e avian retroviruses is blocked at or before viral dna synthesis in restrictive chicken cells. | | 1979 | 229631 |
| [pomacea haustrum (reeve, 1856) as a decoy to infection of biomphalaria glabrata (say, 1818) with schistosoma mansoni sambon, 1907]. | | 1979 | 262318 |
| effects of althesin and its steroidal components on a variety of excised smooth muscle preparations. | | 1979 | 262472 |
| enzyme immunoassay and radioimmunoprecipitation tests for the detection of antibodies to rochalimaea (rickettsia) quintana. | | 1977 | 320604 |
| enzyme immunoassay of antibody to rochalimaea quintana: diagnosis of trench fever and serologic cross-reactions among other rickettsiae. | enzyme immunoassay (eia) tests were used to diagnose trench fever and to determine cross-reactions of rochalimaea quintana with other rickettsiae. the results were compared with those obtained by counterimmunoelectrophoresis (cie). all sera from cases of primary or relapsed forms of trench fever were positive both in eia, with serum antibody titers of 1:20-1:640, and in cie, giving one to three precipitin lines. sera from patients with other rickettsial infections were also tested for reactivity ... | 1978 | 351072 |
| taxonomic relationship of vole agent to rochalimaea quintana. | we investigated the recent claim that the vole agent, a rickettsia-like microorganism isolated from wild voles by baker in 1946, is actually a strain of rochalimaea quintana, the etiological agent of trench fever. the two organisms were compared on the basis of percent guanine-plus-cytosine content, genome size, deoxyribonucleic acid-deoxyribonucleic acid hybridization, polypeptide composition, and serological relationships. although the two organisms do have identical or nearly identical deoxyr ... | 1979 | 393636 |
| effects of nitroglycerin on cardiac function and regional blood flow distribution in conscious dogs. | the effects of intravenous infusion of nitroglycerin (ntg), 8 and 32 microgram/kg.min for 7 min, and of sublingual ntg, 1.2 mg, were examined on direct and continuous measurements of systemic, coronary, and regional hemodynamics, left ventricular (lv) dimensions, pressures, and myocardial contractility in conscious dogs. ntg induced sustained reductions in lv dimensions and transient increases in heart rate and dp/dt, and decreases in mean arterial pressure. initially ntg increased cardiac outpu ... | 1978 | 415619 |
| kinetics of in vitro bovine lymphocyte immunostimulation with a brucella abortus antigen. | a brucella abortus-soluble antigen was investigated, using in vitro assay of lymphocyte immunostimulation, to determine which concentration of this antigen and which period of incubation of the lymphocyte cultures would induce maximum specific lymphocyte immunostimulation as an additional method for further study of b abortus infection in cattle. soluble antigen was prepared from autoclaved cells of b abortus strain 1119-3. peripheral blood lymphocytes were obtained from cattle infected with b a ... | 1978 | 415646 |
| vole agent identified as a strain of the trench fever rickettsia, rochalimaea quintana. | the vole agent described by baker in 1946 was studied as an example of a bacterium that has been mistakenly regarded a rickettsia. unlike rickettsiae, the vole agent killed chicken embryos with great irregularity, multipled primarily at the surface of avian or mammalian cells and not intracellularly, produced colonies rather than plaques on chicken embryo monolayers under agar, and developed small colonies after 4 to 7 days of cultivation on blood plates. it was most conveniently cultivated on m ... | 1978 | 417026 |
| synergy of vancomycin plus cefazolin or cephalothin against methicillin-resistance staphylococcus epidermidis. | the in vitro activity of cephalothin, cefazolin, and vancomycin against 25 isolates of methicillin-resistant staphylococcus epidermidis was determined by means of a broth dilution technique with two sizes of inoculum. the size of the inoculum had a marked effect on the minimal inhibitory concentrations and the minimal bactericidal concentrations of all three antibiotics. with a small inoculum, 100% of the isolates were inhibited by 3.12 micrograms of vancomycin/ml, 76% by 12.5 micrograms of ceph ... | 1979 | 438546 |
| aedes triseriatus and la crosse virus: lack of infection in eggs of the first ovarian cycle following oral infection of females. | la crosse (lac) virus filial infection rates were 0% for 279 first ovarian cycle larvae, 43% for 380 second ovarian cycle larvae, and 58% for 363 third ovarian cycle larvae from orally infected mosquitoes representing 14 wisconsin populations of aedes triseriatus. lac virus was not detected in 72 pools representing 2,250 first ovarian cycle larvae, while 35 pools and 16 pools each containing 30 second and third ovarian cycle larvae, respectively, were all positive for lac virus. similar results ... | 1979 | 484772 |
| [effect of lead on the osmotic fragility of red blood cells in rabbits by the use of coil planet centrifuge (author's transl)]. | | 1979 | 522292 |
| [influence of starvation on the heart rate of rabbits]. | the ecg was recorded in rabbits during eeg-controlled slow wave sleep and during food conditioning sessions. a 2-day food deprivation caused a significant slowing of mean heart rate during sleep. the heart rate increased in hungry animals waiting for food (food expectancy), but did not reach the values of saturated sleeping animals. | 1979 | 525147 |
| [the development and testing of an electronically controlled light sequence bar for the electro oculographic study of the pursuit movement of the human eye (author's transl)]. | | 1979 | 540121 |
| [characteristics of human neutrophils obtained by the chamber method of "skin window"]. | viability, morphology and function of healthy human cells migrated into the chamber through the "skin window" were studied. after 18 to 20 hours there were 42.0 +/- 5.3 (x 10(6))/cm2 viable cells characterized by a high content of mature neutrophils (98.6 +/- 0.6%). normal reactivity of migrated neutrophils was observed using the nitroblue tetrazolium reduction test. the chamber variant of the "skin window" method is recommended as a simple physiological technique for preparing pure populations ... | 1979 | 540158 |
| [effect of aflatoxin b 1 on respiration and oxidative phosphorylation in the rabbit. ii. research on cardiac and renal mitochondria]. | the effect of various concentrations of aflatoxin b1 (ab1) is studied "in vitro" on heart (hm) and kidney mitochondria of rabbit (km). ab1 inhibits (4 x 10(-4)m) the respiratory rate up to a maximum 50-35% in hm and 28-35% in km by glutamate and succinate as substrates respectively, but it does not uncouple oxidative phosphorylation nor does it inhibit site iii (ascorbate + tmpd). the inhibited site appears to be between cytochromes b and c (c1). ab1 seems to be easily transported across heart m ... | 1979 | 548060 |
| morphological and cell association characteristics of rochalimaea quintana: comparison of the vole and fuller strains. | the vole and fuller strains of rochalimaea quintana were grown on monolayers of mouse l cells irradiated 7 days previously and examined by light microscopy and scanning and transmission electron microscopy. most of the bacteria of both strains were shown to adhere to the l cells but remained in an extracellular location. cell division was frequently seen among the extracellular bacteria. the few intracellular bacteria seemed to be within vacuoles and did not multiply. attachment to the eucaryoti ... | 1978 | 681283 |
| effect of specific antibody and complement on the survival of rochalimaea quintana in vitro. | no bactericidal effect was produced when rochalimaea quintana was exposed for 1 h to a combination of high-titered anti-r. quintana rabbit serum and guinea pig complement. | 1978 | 730348 |
| maintenance of spermatogenesis with testosterone or dihydrotestosterone in hypophysectomized rats. | | 1975 | 1151927 |
| comparative morphology of genital cones of genus ancylostoma dubini, 1843. | male worms of ancylostoma braziliense, a. ceylanicum, a. kusimaense, a. malayanum, a. duodenale, a. caninum, a. tubaeforme, agriostomum vryburgi and cyclodontostomum purvisi have the external appendages beside the anogenital aperture. these anogenital structures are morphologically similar and are assumed to be homologous among the species with three pairs of teeth or more. in hookworms with two pairs of teeth, the anogenital structures are complex. anogenital features can be used as a taxonomic ... | 1975 | 1166351 |
| stimulation and inhibition of biosynthesis of prostaglandins in human skin by some hydroxyethylated rutosides. | the effect of some hydroxyethylated rutosides on the biosynthesis of prostaglandins was studied in the microsomal fraction of human skin homogenates. microsomes were incubated with mono-0-hydroxyethyl-7-rutoside (mono-7-hr), di-0-hydroxyethyl-7',4-rutoside (di-hr), tri-0-hydroxyethyl-7,3',4'-rutoside (tri-7,3',4'-hr), tetra-0-hydroxyethyl-5,7,3',4'-rutoside (tetra-hr) and a mixture of hydroxyethylated compounds (hr). prostaglandins were determined by bioassay after organic solvent extraction and ... | 1975 | 1208886 |
| expression and release of neuroregulators during development: monoamines and neuropeptides. | the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ... | 1992 | 1306802 |
| synthesis and degradation of the mrna of the tn21 mer operon. | the mercury resistance locus encoded by tn21 on the monocopy incfii plasmid r100 (mertn21) consists of a metal-responsive activator/repressor, merr, which controls initiation of a polycistronic message that includes genes for the uptake (mertpc) and reduction (mera) of hg2+ and merd, which may also play a minor regulatory role. comparison of the relative abundance of the 5' and 3' ends of the mertpcad transcript revealed a strong transcriptional gradient in the operon, consistent with previous o ... | 1992 | 1317460 |
| new monoclonal antibodies for the detection of immediate early antigens of cytomegalovirus. | two new monoclonal antibodies, cie-1 and cie-2, were developed for the rapid detection of human cytomegalovirus (hcmv) infection. they were found to be reactive with immediate early protein of hcmv in the nuclei of infected fibroblasts, as early as 3 hours post-infection. by radioimmunoprecipitation, cie-1 was found to react with a protein with an apparent molecular weight of 70,000, whereas cie-2 precipitated 2 proteins of 70,000 and 72,000 daltons, respectively. both monoclonal antibodies reco ... | 1992 | 1319172 |
| phylogenetic relationships among the agent of bacillary angiomatosis, bartonella bacilliformis, and other alpha-proteobacteria. | bacillary angiomatosis (ba) and chronic bartonellosis are bacterial infections of humans which result in an unusual vascular proliferative tissue response. in order to determine their phylogenetic relationships, we have determined greater than 95% of the 16s rrna sequences for these two organisms by amplification directly from infected ba tissue and from a bartonella bacilliformis lyophilized culture. the ba agent and b. bacilliformis are closely related alpha-proteobacteria (98.5%), although th ... | 1992 | 1378524 |
| linoleate-dependent co-oxygenation of benzo(a)pyrene and benzo(a)pyrene-7,8-dihydrodiol by rat cytosolic lipoxygenase. | 1. co-oxygenation of 14c-labelled benzo(a)pyrene and benzo(a)pyrene-7,8-dihydrodiol was studied in rat lung cytosol, using linoleic acid as a co-substrate. covalently bound and soluble metabolites were quantified by radiometry and h.p.l.c., respectively. 2. the co-oxygenation resulted in the production of reactive metabolites capable of protein binding as well as a series of soluble derivatives. 3. co-oxygenation of benzo(a)pyrene yielded primarily a significant amount of benzo(a)pyrene-6,12-dio ... | 1992 | 1413876 |
| immunocytochemical identification of rochalimaea henselae in bacillary (epithelioid) angiomatosis, parenchymal bacillary peliosis, and persistent fever with bacteremia. | we report the immunocytochemical identification of rochalimaea henselae, a newly recognized fastidious, gram-negative, warthin-starry-positive organism, as the common pathogen in bacillary angiomatosis (ba), bacillary peliosis (bp) of the liver and spleen, and persistent fever with bacteremia in immunocompromised patients. immunogenic proteins of the r. henselae strain isolated from the blood of a febrile immunocompromised patient with bp of the liver were used to produce primary immune serum in ... | 1992 | 1530106 |
| rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis. | nine strains of rochalimaea spp. that were isolated from patients over a period of 4.5 years were characterized for their enzyme activities, cellular fatty acid compositions, and dna interrelatedness among rochalimaea spp., bartonella bacilliformis, and afipia felis (cat scratch disease bacillus). all except one isolate, which was rochalimaea quintana, were determined to belong to a newly proposed species, rochalimaea henselae sp. nov. after recovery from clinical material, colonies required 5 t ... | 1992 | 1537892 |
| effects of cardioplegic solutions and their components on human saphenous vein contractility. | cardioplegic solution administration into the vein graft is an established method to ensure cardioplegic distribution beyond coronary artery stenoses. the ultrastructural demonstration of severe endothelial damage after cardioplegic exposure suggests that intravenous cardioplegic administration can contribute to early and late graft thrombosis. the direct effect on human saphenous vein contractility of three cardioplegic solutions and their components was compared. a solution with 30 mmol/l k+ a ... | 1992 | 1540064 |
| modulation of macrophage hyperactivity improves survival in a burn-sepsis model. | macrophage hyperactivity with increased production of tumor necrosis factor, interleukin 6, interleukin 1, and prostaglandins has been demonstrated in the injured patient, but the effect of this on the clinical outcome is unclear. we studied the effect of combination interleukin 1 beta and indomethacin sodium therapy on macrophage hyperactivity and survival after sepsis in a murine burn model. macrophage interleukin 1, interleukin 6, and tumor necrosis factor alpha production were all significan ... | 1992 | 1540091 |
| rochalimaea henselae causes bacillary angiomatosis and peliosis hepatis. | recent studies have demonstrated that a newly described agent of persistent bacteremia, rochalimaea henselae, and the agent of bacillary angiomatosis are both closely related to rochalimaea quintana. bacillary peliosis hepatis seemed likely to have the same etiologic agent as bacillary angiomatosis. we sought these pathologic changes in patients from whom r henselae was cultivated. | 1992 | 1546924 |
| bacillary angiomatosis. a "new" disease with a broadening clinicopathologic spectrum. | bacillary angiomatosis (ba) is a reactive vasoproliferative lesion occurring almost exclusively in immunocompromised individuals in response to infection by a bacillus closely related to rochalimaea quintana. the commonest site of involvement is the skin, in the form of multiple erythematous nodules, but bacillary angiomatosis can also present in a wide variety of sites such as soft tissues, bone, lymph node, liver and spleen. some patients may present with persistent fever and bacteraemia. baci ... | 1992 | 1576430 |
| is erythromycin indicated in pregnancy? | | 1992 | 1594870 |
| murine antibody responses distinguish rochalimaea henselae from rochalimaea quintana. | rochalimaea henselae causes persistent bacteremia, bacillary angiomatosis, and parenchymal bacillary peliosis. detection of a specific antibody response to r. henselae infection may represent an alternative to cultivation as a means of diagnosis. we assessed the specificity of induced murine antibodies for antigens from r. henselae and the closely related species r. quintana. groups of cd-1 mice were inoculated with whole organisms of six strains of r. henselae and two of r. quintana. pre- and p ... | 1992 | 1629326 |
| oxidative inactivation of alpha 1-proteinase inhibitor by alveolar macrophages from healthy smokers requires the presence of myeloperoxidase. | the aim of this work was to study the ability of human alveolar macrophages (am) of 10 healthy smokers to inactivate alpha 1-proteinase inhibitor (alpha 1pi). purified alpha 1pi was incubated for 45 min, with human alveolar macrophages before and after stimulation by phorbol myristate acetate (pma) or opsonized zymosan. as a positive control, the same experiments were performed in parallel with blood human neutrophils (pmn). results are expressed as percentage of inactivation of alpha 1pi as eva ... | 1991 | 1657063 |
| genetics of rickettsiae. | classical genetic approaches useful with free-living bacteria are difficult to apply to the rickettsiae. although rickettsial mutants have been isolated over the years, the genetic basis of these mutants is unknown, limiting their usefulness. the application of molecular biological techniques to rickettsial studies has provided the opportunity to isolate and study specific genes. genes encoding metabolic enzymes from rickettsiae were cloned in escherichia coli and shown to retain their regulator ... | 1991 | 1679397 |
| transformation and genomic restriction mapping of rochalimaea spp. | transformation procedures using electroporation were established for rochalimaea quintana. several cosmid/plasmids possessing the rk2 or rsf1010 origin of replication were successfully inserted. plasmid retention and replication were verified by antibiotic resistance and southern blot analysis. the highest level of transformation was obtained at a voltage field strength of 12.5 kv/cm with a pulse time of 10 miliseconds. transformation efficiency was low (0.3%) with approximately 10(5) transforma ... | 1991 | 1687634 |
| immunocytochemical localization of follicle stimulating hormone in normal human stomach. | immunoreactive follicle-stimulating hormone (ir-fsh) is detected in sections of formalin-fixed and paraffin-embedded gastric mucosal tissue of normal men, using the immunoperoxidase staining technique and specific antisera to hfsh (niddk, nih). positive staining for ir-fsh was detected in the parietal cells lining the gastric glands of the intermediate zone. the staining was intracytoplasmic and distributed throughout the cytoplasm. ir-fsh was also found to be present in the basal part of the fo ... | 1990 | 1695496 |
| molecular characterization and proposal of a neotype strain for bartonella bacilliformis. | bartonella bacilliformis, the etiologic agent of bartonellosis, was characterized biochemically and by dna hybridization, guanine-plus-cytosine content, genome size, and 16s rrna sequencing. dnas from the two strains in our collection exhibited 97% relatedness in hydroxyapatite reactions done at 55 degrees c (optimal reassociation criterion) and 100% relatedness in reactions done at 70 degrees c (stringent reassociation criterion). there was no evidence of divergence within the related sequences ... | 1991 | 1715879 |
| 16s rrna sequences of bartonella bacilliformis and cat scratch disease bacillus reveal phylogenetic relationships with the alpha-2 subgroup of the class proteobacteria. | the primary structures of 16s rrnas of bartonella bacilliformis, an isolate of the cat scratch disease (csd) bacillus, and a strain phenotypically similar to the csd bacillus were determined by reverse transcriptase sequencing. these microorganisms were found to be members of the alpha-2 subgroup of the class proteobacteria. the sequence from b. bacilliformis was most closely related to the rrna of rochalimaea quintana (91.7% homology), the etiologic agent of trench fever. the sequence from the ... | 1991 | 1719021 |
| dermatopathologic findings in patients infected with hiv. | several inflammatory, infectious, and neoplastic conditions in hiv-infected patients are distinctive or require a biopsy for diagnosis. some differ subtly from similar conditions seen in noninfected patients. the exanthem of acute hiv infection cannot be diagnosed specifically on biopsy as its histologic appearance is similar to that of other viral exanthemata. a condition that closely resembles seborrheic dermatitis occurs in hiv-infected patients. plasma cells, necrotic keratinocytes, and leuk ... | 1992 | 1730173 |
| taxonomic considerations of bartonella bacilliformis based on phylogenetic and phenotypic characteristics. | the 16s-rrna gene of bartonella bacilliformis was amplified using the polymerase-chain reaction (pcr). the amplification product was sequenced using a linear-pcr procedure and compared with other published 16s-rrna sequences. the results of this analysis placed b. bacilliformis in the alpha subgroup of the proteobacteria, and more specifically demonstrated its close phylogenetic relationship to rochalimaea quintana. this relationship is supported by similarities in the size and mean base composi ... | 1991 | 1778432 |
| hepatoprotector effect of chrysanthemum balsamita extract in ethanol intoxicated rats. | male wistar rats weighing 200 +/- 10 g were intoxicated with ethanol 50% (1 ml/100 g b.w./, day, for 12 days) and concomitantly treated with hydroethanol extract of chrysanthemum balsamita. an increase of liver glucose-6-phosphatase and phosphorylase a activities correlated with a liver glycogen breakdown as well as a decrease of glycemia were obtained in rats given ethanol 50%. it was found that chrysanthemum balsamita extract counteracted ethanol effects and maintained at normal limit the meta ... | 1991 | 1817666 |
| in vitro folding pathway of phage p22 tailspike protein. | the intracellular chain folding and association pathway of the thermostable, trimeric phage p22 tailspike endorhamnosidase has been the subject of a previous detailed study employing temperature-sensitive folding mutants. recently, reconstitution of native tailspikes from completely unfolded polypeptides has been accomplished, providing a model system to compare protein folding pathways in vivo and in vitro. the in vitro reconstitution pathway of the protein after dilution from guanidine hydroch ... | 1991 | 1828991 |
| in vitro folding pathway of phage p22 tailspike protein. | the intracellular chain folding and association pathway of the thermostable, trimeric phage p22 tailspike endorhamnosidase has been the subject of a previous detailed study employing temperature-sensitive folding mutants. recently, reconstitution of native tailspikes from completely unfolded polypeptides has been accomplished, providing a model system to compare protein folding pathways in vivo and in vitro. the in vitro reconstitution pathway of the protein after dilution from guanidine hydroch ... | 1991 | 1828991 |
| the sertoli-spermatid processes in the mouse: new cellular structure. | in addition to the known sertoli-cell processes, processes of the mouse spermatid's cytoplasm are found to invaginate neighbouring spermatids. surrounded by the adjacent sertoli process, the spermatid processes form a "spermatid-sertoli cell process". they are observed between spermatids at the same step or at different steps of their development and degenerate mostly at step 13 to 15 of spermiogenesis. whether these structures are related to either spermatid exchanges or connections or particip ... | 1991 | 1933027 |
| effects of acute exercise and prolonged exercise training on blood pressure, vasopressin and plasma renin activity in spontaneously hypertensive rats. | the purpose of this study was to investigate the effect of swimming training on systolic blood pressure (bps), plasma and brain vasopressin (avp), and plasma renin activity (pra) in spontaneously hypertensive rats (shr) during rest and after exercise. resting and postexercise heart rate, as well as blood parameters such as packed cell volume (pcv), haemoglobin concentration (hb), plasma sodium and potassium concentrations ([na+], [k+]) osmolality and proteins were also studied. hypophyseal avp h ... | 1991 | 2044526 |
| reduction of drug accumulation and dna topoisomerase ii activity in acquired teniposide-resistant human cancer kb cell lines. | we have isolated stable teniposide (vm26)-resistant cell lines from human cancer kb cells by stepwise exposure to increasing doses of the drug. at each step, we have purified vm26-resistant cell lines. kb/vm-a, kb/vm-b, kb/vm-1, kb/vm-2, kb/vm-3, and kb/vm-4 showed 3-, 6-, 12-, 16-, 74-, and 95-fold higher resistance to vm26 than did kb. we have further characterized kb/vm-2 and kb/vm-4 which showed about 15- and 100-fold higher resistance to vm26 or etoposide (vp16) than did kb. both vm26-resis ... | 1990 | 2168282 |
| in vitro assembly of relaxosomes at the transfer origin of plasmid rp4. | during initiation of conjugative transfer of dna containing the transfer origin (orit) of the promiscuous plasmid rp4, the proteins trai, traj, and trah interact and assemble a specialized nucleoprotein complex (the relaxosome) at orit. the structure can be visualized on electron micrographs. site- and strand-specific nicking at the transfer origin in vitro is dependent on the proteins trai and traj and on mg2+ ions. substrate specificity is directed exclusively towards the cognate transfer orig ... | 1990 | 2168553 |
| structure of the human centromere at metaphase. | until recently the centromere was thought to be a relatively homogeneous region of densely packed heterochromatin with a single differentiated domain--the kinetochore--at its surface, representing the point of attachment of the mitotic spindle. we now know that the centromere of higher eukaryotes is composed of several domains that have been identified using antibody probes. somewhere within the domains are located both the factor(s) that control the disjunction of sister chromatids and the mole ... | 1990 | 2193435 |
| the agent of bacillary angiomatosis. an approach to the identification of uncultured pathogens. | bacillary angiomatosis is an infectious disease causing proliferation of small blood vessels in the skin and visceral organs of patients with human immunodeficiency virus infection and other immunocompromised hosts. the agent is often visualized in tissue sections of lesions with warthin-starry staining, but the bacillus has not been successfully cultured or identified. this bacillus may also cause cat scratch disease. | 1990 | 2233945 |
| a newly recognized fastidious gram-negative pathogen as a cause of fever and bacteremia. | we identified a motile, curved, gram-negative bacillus as the cause of persistent fever and bacteremia in two patients with symptomatic human immunodeficiency virus infection. the same organism was subsequently recovered from a bone marrow-transplant recipient with septicemia and from two immunocompetent persons with week-long febrile illnesses. all the patients recovered after antimicrobial therapy. | 1990 | 2233947 |
| [the cultivation of hybridoma cells in agarose granules]. | the results of cultivation of agarose gel immobilized hybridoma cells producing monoclonal antibodies to human alpha 2-interferon are presented. the immobilized cultured cells retained the higher viability for 10-28 days without changing the growth medium, in comparison with cultured cells in control suspension. the cells immobilized in agarose gel demonstrated a tendency to polyploidization as was revealed by the technique of dna cytofluorimetry with hoechst 33258. | 1990 | 2238110 |
| [the cultivation of hybridoma cells in agarose granules]. | the results of cultivation of agarose gel immobilized hybridoma cells producing monoclonal antibodies to human alpha 2-interferon are presented. the immobilized cultured cells retained the higher viability for 10-28 days without changing the growth medium, in comparison with cultured cells in control suspension. the cells immobilized in agarose gel demonstrated a tendency to polyploidization as was revealed by the technique of dna cytofluorimetry with hoechst 33258. | 1990 | 2238110 |
| presence of specific binding sites for [3h]sarcophytol-a in cultured human skin fibroblasts. | the presence of specific binding sites for [3h]sarcophytol-a in human skin fibroblasts was examined using biochemical and morphological methods. the displacement studies clearly revealed that high (kd = 31.0 nm) and low (kd = 6.05 microm) affinity sites were present in the intact cells. moreover, autoradiographic studies using light microscopy revealed that the specific binding sites may exist in both the cytoplasm and the nuclei. | 1990 | 2347400 |