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[effect of a beryllium complex on growth of pseudomonas fluorescens (types r and s). ii. competition with magnesium].a study of the inhibitory action of beryllium on the growth of pseudomonas fluorescens reveals that the observed effect can be partly explained by competition between beryllium and magnesium in various processes which are indispensable to cellular metabolism. in addition, an "adaptation" phenomenon is observed which appears to be based on the selection of cells which are more highly resistant towards the inhibitor.1975126779
oral susceptibility of aedes albopictus to dengue type 2 virus: a study of infection kinetics, using the polymerase chain reaction for viral detection.female aedes albopictus mosquitoes, aged 1 week, were infected with den-2 dengue virus. the kinetics of infection in mosquito brain and mesenteron were monitored using dna probes with polymerase chain reaction (pcr) amplification of target dna sequences coding for den-2 virus envelope protein, compared with the standard immunofluorescence assay technique (ifa). rates of virus detection in the mesenteron of orally infected mosquitoes rose to 38% by day 4 post-inoculation, then declined until day ...19921463895
purification and characterization of the major group-specific core antigen vp7 of bluetongue virus synthesized by a recombinant baculovirus.the major core protein, vp7, of bluetongue virus serotype 10 (btv-10) has been purified from insect cells infected with a genetically manipulated recombinant baculovirus. the high level expression of vp7 (in excess of 100 mg per litre of culture) and its presence in the soluble fraction of infected cells following lysis by detergent has allowed the purification of the protein virtually to homogeneity (95%) by a simple two-step procedure of ammonium sulphate fractionation and ion-exchange chromat ...19902174958
disappearance of a putative da-neuron antibody following adrenal medulla transplantation: relationship to a striatal-derived da neuron trophic factor. 19902290972
effect of a trypsin inhibitor from soybeans (bowman-birk) on the secretory activity of the human pancreas.this study was undertaken to clarify the issue of whether feedback regulation of pancreatic enzyme secretion occurs in humans. a naturally occurring inhibitor of trypsin and chymotrypsin, the bowman-birk inhibitor of soybeans, was used to reduce the activities of these enzymes normally secreted by the pancreas into the duodenum. pure pancreatic juice was collected by endoscopic retrograde cannulation of the pancreatic duct in a protocol consisting of three periods: period 1 (15 min), collections ...19882446949
rapid diagnosis of bovine herpesvirus encephalitis: comparison of nucleic acid hybridization and immunoperoxidase methods using clinical samples. 19882844043
[experimental study of the activity of a number of enzyme-substrate systems after inhalation of aluminum-cobalt-molybdenum catalyst]. 19902116994
taxonomy of leishmania. use of isoenzymes. suggestions for a new classification.the authors propose a new classification for the genus leishmania ross, 1903 based both on the use of intrinsic and extrinsic characters and on linnean and adansonian methods. the type of vertebrate host makes it possible to recognize the genus group: leishmania designates kinetoplastida parasites of mammals. neighbouring forms which parasite reptiles are now grouped in the genus sauroleishmania ranque, 1973. characteristics of the intravectorial cycle (supra- and peri-pyloric) are used to defin ...19902080829
electroencephalographic study of rat hyperthermic seizures.the behavior and eeg of rat hyperthermic convulsions was studied. we found that a long run of high-voltage slow waves without convulsions, high-voltage irregular activity consisting of spikes and waves with intermittent myoclonus, and rapid spike and wave bursts accompanied by generalized clonic convulsions occurred sequentially. this behavioral and eeg activity is analogous to human febrile convulsions and supports the usefulness of this model for studies of human febrile convulsions. rats freq ...19912044491
transiently catecholaminergic (tc) cells in the bowel of the fetal rat: precursors of noncatecholaminergic enteric neurons.experiments were done to study the fate of transient catecholaminergic (tc) cells that develop in the rodent gut during ontogeny. when they are first detected, at day e11 in rats, tc cells are distributed along the vagal pathway, in advance of the descending fibers of the vagus nerves, and in the foregut. the early tc cells coexpress the immunoreactivities of several neural markers, including 150-kda neurofilament protein, peripherin, microtubule associated protein (map) 5, and growth-associated ...19901976556
enhancement of dynorphin gene expression in spinal cord following experimental inflammation: stimulus specificity, behavioral parameters and opioid receptor binding.the stimulus specificity for enhancement of dynorphin gene expression in rat spinal cord was studied by combined measurements of the peptide dynorphin a 1-8 and preprodynorphin mrna levels during peripheral inflammation induced by several agents. the density of kappa receptors, the putative receptor for dynorphin peptides, was examined using receptor binding with autoradiographic visualization. mu and delta receptor classes were also studied. all inflammatory agents tested (carrageenan, phorbol ...19882906426
location of phosphotyrosine-containing proteins by immunocytochemistry in the rat forebrain corresponds to the distribution of the insulin receptor.cellular regulation by certain growth factor receptors and protooncogene products involves tyrosine kinase activity with the resultant tyrosine phosphorylation of protein substrates. in the present report we describe the distribution of phosphotyrosine-containing material detected by immunocytochemistry (icc) in the rat forebrain. specificity of the affinity-purified antibody against phosphotyrosine used in the icc technique was demonstrated by the ability of phosphotyrosine and p-nitrophenyl ph ...19901693770
lidocaine inhibits prolactin secretion in gh4c1 cells by blocking calcium influx.the mechanism of the inhibitory effect of local anesthetics on hormone secretion was studied in the gh4c1 line of rat pituitary tumor-derived cells. lidocaine between 0.1 and 5 mm exerted significant dose-dependent inhibition on the increment in cytosol ca2+ concentration ([ca2+]i) and prolactin (prl) secretion induced by 30 mm k+. for both effects the ic50 was 0.25 mm and maximal inhibition occurred at 5 mm. a normal response returned within 20 min after removal of lidocaine from the incubation ...19921280232
development of ancylostoma ceylanicum looss, 1911 (hamster strain) in the albino mouse, mus musculus, with and without cortisone.complete development of ancylostoma ceylanicum (hamster strain) is reported for the first time in mice. a patent infection (viz. ova in faeces) could be established only under the influence of hydrocortisone acetate. cultures made from the faeces containing eggs produced viable larvae which were infective to mice.19751187181
[the future of viral hepatitis vaccines]. 19852990115
visual-vestibular responses in vestibular nuclear neurons in the intact and cerebellectomized, alert cat. 1979514510
estradiol-17 beta stimulates basal and thyrotropin releasing hormone induced prolactin secretion by bovine pituitary cells in primary culture. 1979111979
clonospecific structural heterogeneity in the thy-1 molecule from mouse t lymphocytes.the thy-1 molecule immunoprecipitated from detergent-solubilized, 125i-labeled cell-surface proteins was shown to be processed in two distinct ways by mouse t lymphocytes: one leading to the expression by thymocytes, concanavalin a-activated spleen blasts, and six of nine t-cell clones of a molecule of 25-28 kd, and another, observed in three other t-cell clones, leading to the expression at their surface of a so far undescribed low mr (23 kd) form of thy-1. the results of two-dimensional gel el ...19852860061
clonospecific structural heterogeneity in the thy-1 molecule from mouse t lymphocytes.the thy-1 molecule immunoprecipitated from detergent-solubilized, 125i-labeled cell-surface proteins was shown to be processed in two distinct ways by mouse t lymphocytes: one leading to the expression by thymocytes, concanavalin a-activated spleen blasts, and six of nine t-cell clones of a molecule of 25-28 kd, and another, observed in three other t-cell clones, leading to the expression at their surface of a so far undescribed low mr (23 kd) form of thy-1. the results of two-dimensional gel el ...19852860061
in vitro motility of isolated adults and segments of onchocerca volvulus, brugia pahangi and acanthocheilonema viteae.observations were made on the spontaneous motility in vitro of entire adult worms and segments of onchocerca volvulus, brugia pahangi and acanthocheilonema viteae. segment motility was recorded in a mechano-transducer apparatus and responses of worm tissues to anthelmintics and other pharmacological agents were compared. entire adult female o. volvulus showed varied patterns of motor activity, ranging from continuous low level whole body motion to periodic spasmodic contractions interspersed bet ...19882852396
an electrophysiological analysis of the effect of reactive blue 2, a putative p2-purinoceptor antagonist, on inhibitory junction potentials of rat caecum.the electrophysiological effects of the putative p2-purinoceptor antagonist reactive blue 2 (rb2) were investigated in strips of circular muscle of rat caecum with the sucrose gap technique. rb2 (0.1-1 mm) antagonized in a concentration-dependent manner, the amplitude, rate of rise and speed of onset of the inhibitory junction potentials elicited either with single pulse or with train of field stimulation at 10 hz. a fully effective concentration of rb2 (0.5 mm) decreased the membrane response t ...19863019730
an electrophysiological analysis of the effect of reactive blue 2, a putative p2-purinoceptor antagonist, on inhibitory junction potentials of rat caecum.the electrophysiological effects of the putative p2-purinoceptor antagonist reactive blue 2 (rb2) were investigated in strips of circular muscle of rat caecum with the sucrose gap technique. rb2 (0.1-1 mm) antagonized in a concentration-dependent manner, the amplitude, rate of rise and speed of onset of the inhibitory junction potentials elicited either with single pulse or with train of field stimulation at 10 hz. a fully effective concentration of rb2 (0.5 mm) decreased the membrane response t ...19863019730
copy choice illegitimate dna recombination.precise excision of tn10 and related transposons occurs by recombination between directly repeated 9 bp sequences that flank the transposon. the excision, which is a model for a class of illegitimate dna recombination events, was stimulated 10(6) times by induction of single-stranded dna synthesis, occurred during conversion of single-stranded dna to double-stranded form, and entailed no transfer of physical material from parental to progeny molecules. we conclude that it occurred by copy choice ...19882832066
expression of glutathione s-transferase isoenzymes in human small cell lung cancer cell lines.a glutathione s-transferase (gst) isoenzyme having common antigenicity to rat placental form (gst-p) and human placental form (gst-pi) has recently been suggested may be a marker of carcinogenesis. in the present study we have investigated the expression of this isoenzyme in three small cell lung cancer cell lines in order to determine whether or not this isoenzyme can be used as a general marker of carcinogenesis. gst activity towards 1-chloro-2,4-dinitrobenzene in two of the cell lines (nes an ...19882826036
the effectiveness of bronchoscopy in the diagnosis of pneumocystis carinii and cytomegalovirus pulmonary infections in acquired immunodeficiency syndrome.we evaluated the sensitivity of bronchoscopy for the diagnosis of pneumocystis carinii and cytomegalovirus pulmonary infections in patients with acquired immunodeficiency syndrome. the antemortem and postmortem diagnoses were compared in 36 patients who underwent fiberoptic bronchoscopy within two weeks of death. in autopsy-proved cases of pneumocystis carinii pneumonia (pcp), the organism was correctly identified antemortem in 22 (88%) of 25 cases, including 94% of adequate transbronchial bronc ...19873030223
differential effects of steroid hormones on parameters of cell growth.steroid hormones affect the growth of many tumor cells both in vivo and in vitro. growth of cells in vitro can be studied as either anchorage dependent (monolayer culture) or anchorage independent (suspension culture), and in each case, steroid hormones can alter log-phase proliferation rate, saturation density, or cell morphology. results presented here demonstrate that different steroid hormones can have different effects on each of these parameters in breast cancer cells. in androgen-responsi ...19873032417
production of laudanosine following infusion of atracurium in man and its effects on awakening.twenty patients were given maximal doses of atracurium or vecuronium by infusion during surgery. anaesthesia was maintained with an infusion of thiopentone, nitrous oxide and fentanyl. in patients administered atracurium, the plasma laudanosine concentration at cessation of surgery was 0.34 (sd 0.22) micrograms ml-1; there was little tendency to cumulate during operation. a 20% higher arterial concentration of thiopentone was found at awakening in patients given atracurium, suggesting cns stimul ...19892765346
[basis for the maximum allowable concentration of thiourea in the air of the work zone]. 19863084337
[basis for the maximum allowable concentration of thiourea in the air of the work zone]. 19863084337
identification of carbohydrate moieties involved in edta-stable or edta-sensitive cell contact of dictyostelium discoideum.antisera against purified contact site a glycoprotein, with an apparent molecular weight of 80 x 10(3) (80 kda), from dictyostelium discoideum were raised by using freund's adjuvant (antiserum-a) and by using alu-gel-s (antiserum-b) as immunoadjuvants. they were converted into fab fragments for the cell agglutination assay. fab fragments of antiserum-b inhibited only edta-stable cell contact, whereas fab fragments of antiserum-a (fab-a) inhibited edta-sensitive cell contact as well as edta-stabl ...19873115971
central baroreflex regulation by the renal nerves in anesthetized rats.to determine whether the renal nerves affect central baroreflex regulation, the aortic depressor nerve (adn) was stimulated electrically, while blood pressure, heart rate, and splanchnic nerve activity were recorded in renal denervated and sham-operated rats anesthetized with urethane. tail cuff systolic pressure fell 6 days after renal denervation, but mean blood pressure recorded after anesthetizing with urethane did not differ between renal denervated and sham-operated rats. urinary sodium ex ...19892761074
[cerebrovascular incidents in 4 hemodialysis patients treated with erythropoietin].human recombinant erythropoietin (r-huepo) is very effective in the treatment of anemia of hemodialyzed patients. we describe 4 patients who developed symptoms of central nervous dysfunction during r-huepo therapy. three exhibited typical hypertensive encephalopathy, whereas signs of cerebral ischemia were found in the fourth. the increase in blood viscosity with r-huepo treatment, leading to a rise in peripheral vascular resistance and blood pressure especially in previously hypertensive patien ...19883187475
time of application of negative pressure pulses and upper airway muscle activity.effect of upper airway pressure changes on thoracic inspiratory muscles has been shown to depend on the time of application during the breathing cycle. the present study was designed to investigate the importance of the time of application of upper airway negative pressure pulses on upper airway muscles. the upper airway was functionally isolated into a closed system in 24 anesthetized spontaneously breathing rabbits. negative pressure pulses were applied in early (within the first 200 ms) and l ...19892759965
possible role of ammonia in experimental cancer anorexia.plasma concentrations of ammonia were elevated significantly in tumor-bearing rats prior to the onset of anorexia and continued to increase as the tumor grew and anorexia developed. associated with this hyperammonemia were elevated levels of brain glutamine and large neutral amino acids (phenylalanine, tyrosine, tryptophan, methionine, histidine). concentrations of the dopamine metabolites, dopac or hva were elevated in the corpus striatum, nucleus accumbens, hypothalamus and amygdala of anorect ...19892731036
the occurrence of ectopic pregnancy with and without clomiphene citrate use in assisted and nonassisted reproductive technology.an eleven-year review of treatment records of patients who became pregnant as a result of infertility treatment (n = 3692) was conducted. all records were examined to determine whether clomiphene citrate (cc) was used in the cycle of conception and whether or not an ectopic pregnancy occurred. data were additionally analyzed for the incidence of ectopic pregnancy with and without assisted reproductive technology (art). the incidence of ectopic pregnancy was similar between cc (3.4%) and non-cc ( ...19892698906
bombesin-induced hypothermia in the insulin-treated rat: effect on tail-skin temperature.bombesin-like peptides are widely distributed in the mammalian central nervous system and appear to participate in the regulation of a variety of autonomic functions. bombesin has been shown to alter feeding behavior, locomotor activity, and thermoregulation. microinfusion of bombesin into the preoptic area of the hypothalamus produces a reduction in core body temperature, but only if the rat has been cold-exposed, food-deprived, or pretreated with insulin. the mechanism for bombesin-induced hyp ...19892695893
[virus diseases of the nervous system in selected patients at a neurologic specialty unit during a 9-year period].during a 9-year period an unselected population of 377 patients with clinically suspected viral diseases of the nervous system were hospitalized. due to the serological findings a definite viral etiology could be established in 123 (33%). with respect to clinical syndromes serological evidence of viral etiology was found in 74% of mono- respectively oligoneuritides, 43% of meningoencephalitides, whereas in polyneuritis only in 18%. concerning different virus strains varicella zoster virus appear ...19883222963
resistance control in hypertension.in hypertension the small arteries undergo structural changes that increase vascular resistance, both in vivo and in vitro. the hallmark of a physiological vascular amplifier is that enhanced resistance responses must occur about the resting value. for this to happen, the average radius of the resistance vessels must be narrower than normal; increased wall thickness without narrowing does not result in this type of amplification. in primary hypertension in spontaneously hypertensive rats (shr), ...19892681590
whole rat embryos require methionine for neural tube closure when cultured on cow serum.headfold-stage rat embryos, when cultured on cow serum without supplemental methionine, failed to close their neural tubes, lacked eyes and branchial arches, were abnormally shaped and were reduced in protein content compared to methionine-supplemented embryos. methionine was essential during the first 18 h of culture, a period in which neural tube closure was initiated in supplemented cultures. all cow serum samples tested were found to require methionine addition, and the methionine was not re ...19892600678
hypogammaglobulinemia and depressed natural killer cell cytotoxicity in a patient with pneumocystis carinii infection. 19883263615
the prevalence of hantaan viruses among rattus norvegicus in tokyo in the 1960's.during the 1960's, 15 out of 89 rats (16.8%) captured in the port area and 6 out of 59 rats (10.2%) in the suburb of tokyo were seropositive against hantaan viruses.19892573224
effect of tidal volume and peep on rate of edema formation in in situ perfused canine lobes.the effects of raising tidal volume and positive end-expiratory pressure (peep) on rate of edema formation were studied in in situ canine left upper lobe preparations. edema was induced by increasing blood flow to the left upper lobe (4-8 times normal). in the same animal, at equivalent flows and microvascular hydrostatic pressures, rate of edema formation observed with larger tidal volumes was significantly higher than that observed with smaller tidal volumes (0.73 +/- 0.29 vs. 0.58 +/- 0.30, p ...19883292503
[electrical and contractile properties of the smooth muscle of the femoral artery in the rabbit]. 19872446933
primate specific sialoglycoprotein of sperm head plasma membrane defined by an anti-carbohydrate monoclonal antibody.a sialoglycoprotein, an integral component of the head plasma membrane of human spermatozoa, is recognized by the a-hs 1a.1 monoclonal antibody. the antigenicity is associated with the sugar moiety since: a) trypsin digestion did not affect the antigenic determinant; b) pretreatment of the cells with beta-glucosidase, alpha-mannosidase and neuraminidase completely abolished antibody binding. endoglycosidase d and glycopeptidase f were inactive. the a-hs 1a.1 did not recognize a variety of blood- ...19873317219
testing for chlamydia trachomatis: objective criteria for recommendations for screening using nonculture techniques. 19873318161
nad+-dependent retinol dehydrogenase in liver microsomes.a microsomal nad+-dependent retinol dehydrogenase is being described with optimal activity at physiological ph. the enzyme was present in liver microsomes of rats and also in a strain of deermice which lacks the cytosolic retinol dehydrogenase. unlike the latter enzyme, the microsomal retinol dehydrogenase was not inhibited by either ethanol or 4-methylpyrazole; its activity was insensitive to co and not oxygen dependent, in contradistinction with that of the microsomal cytochrome p-450 and nadp ...19873322195
nmr measurements of intracellular sodium in the rabbit proximal tubule.the present study evaluated the use of nuclear magnetic resonance (nmr) spectroscopy to monitor directly and continuously intracellular sodium levels in rabbit renal cortical tubule suspensions. when the paramagnetic shift reagent dysprosium tripolyphosphate was added to the extracellular medium it was possible to resolve signals from intracellular and extracellular sodium without adversely affecting cellular viability. an efflux of intracellular sodium against a significant concentration gradie ...19852409818
lymphocytes proliferation kinetics and sce variation after rubella vaccination.the effect of anti-rubella vaccination on lymphocyte proliferation kinetics in vitro and on sce frequency was studied in three young women. the studies were carried out by taking blood samples before vaccination (day 0) and subsequently on days 7, 14, 28 and 42. the mitotic index (mi) shows a decrease at day 14 and 28 followed at day 42 by an increase above day 0 levels. the average cell cycle (acc) shows a decrease at day 14 followed by an increase at day 28. complex variations were also found ...19883352628
differential effects of adrenergic agonists and phorbol esters on the alpha 1-adrenoceptors of hepatocytes and aorta.epinephrine, norepinephrine and phenylephrine stimulate phosphatidylinositol labeling with [32p]pi in both rat hepatocytes and rabbit aorta. methoxamine was a full agonist for this effect in rabbit aorta whereas cirazoline and oxymetazoline were partial agonists. in contrast, these three agents (methoxamine, cirazoline and oxymetazoline) were unable to stimulate phosphatidylinositol labeling in rat hepatocytes. furthermore, cirazoline and oxymetazoline were able to displace the dose-response cur ...19852862055
binding parameters and microbiological activity of macrolides, lincosamides and streptogramins against staphylococcus aureus.parameters of erythromycin binding to staphylococcus aureus were measured in-vitro using an equilibrium method with [3h]erythromycin. the dissociation constant of the complex, erythromycin-s. aureus sensitive strain, was kd = 0.11 microm. the maximal binding, representing the density of binding sites was 14,847 molecules/cell. no binding was detectable on the constitutive resistant strain. macrolides, streptogramins and lincosamides displaced bound [3h]erythromycin by a competitive process indic ...19872884302
somatostatin release from dispersed hypothalamic cells: effects of diabetes.we examined the release of growth hormone-release inhibiting factor (somatostatin) from dispersed hypothalamic cells obtained from mature diabetic rodents and normal age-matched controls, in an attempt to demonstrate a possible hypothalamic defect which might underlie some of the reported abnormalities in somatotrophic function in diabetes mellitus. insulinopoenic diabetes was induced by either streptozotocin or alloxan. somatostatin release from cells from diabetic rats was diminished both basa ...19872895719
adsorptive endocytosis of lysosomal enzymes by human fibroblasts: presence of two different functional systems that deliver an acid hydrolase to lysosomes.endocytosis of human spleen beta-glucuronidase by human fibroblasts can be completely impaired by the competitive inhibitor mannose 6-phosphate or by pretreatment with acid phosphatase or endoglycosidases h or f. however, endocytosis of bovine spleen and liver beta-glucuronidase is partially impaired by the same treatments, suggesting that the bovine enzyme contains two endocytosis recognition markers located in separate enzyme domains. the mannose 6-phosphate recognition marker seems to be resp ...19892913951
physicochemical characterization of [3h] dhea binding in rat liver.dehydroepiandrosterone (dhea), the native clinical steroid and steroid precursor, may have a targeted physiologic role. a high affinity (kd 2.3nm) and steroid specific [3h] dhea binding macromolecule in male sprague-dawley rat hepatic cytosol suggests that dhea may have receptor mediated physiologic action. 3[h] dhea binding was highest in the liver followed by kidney and testis cytosols. sulfhydryl reagents such as n-ethylmalemide and iodoacetamide inhibited the binding of [3h]dhea by up to 60- ...19882972284
abnormalities of erythrocyte membrane fatty acid composition in human essential hypertension.the fatty acid content of erythrocyte plasma membrane was estimated in cells of 17 untreated hypertensive patients, and compared to that of 16 control subjects matched for age, sex and weight. mean linoleic acid (18:2) content was significantly lower in hypertensives than controls, whereas arachidonic acid (20:4) and oleic acid (18:1) were significantly higher. these results provide further evidence for an alteration in the physiochemical structure of the plasma membrane in cells of hypertensive ...19873508198
expression of human choriogonadotropin in monkey cells using a single simian virus 40 vector.we have inserted the cdnas coding for both polypeptide subunits, alpha and beta, of human choriogonadotropin (hcg) into a single simian virus 40 expression vector in such a way that they replace the viral vp2 and vp1 coding sequences, respectively. monkey cells infected with this virus and the appropriate helper virus produce dimeric hcg. hcg produced in this system was shown to chromatograph identically to standard hcg preparations on gel filtration and to be biologically active in the mouse ut ...19852987938
binding and degradation of 125i-gastrin by plasma membranes from homogenized rat gastric mucosa.binding of 125i-gastrin to the 270-30,000 g fraction from homogenized rat oxyntic mucosa was studied. 'specific' binding was calculated by subtracting the binding at excess cold gastrin from the binding with labelled gastrin (250 pm) only. at 30 degrees c specific binding rose rapidly to a short-lived maximum before falling gradually, whereas at 15 degrees c and 0 degree c specific binding rose gradually to a higher plateau level. the reduced binding at 30 degrees c could be caused by degradatio ...19863018914
binding and degradation of 125i-gastrin by plasma membranes from homogenized rat gastric mucosa.binding of 125i-gastrin to the 270-30,000 g fraction from homogenized rat oxyntic mucosa was studied. 'specific' binding was calculated by subtracting the binding at excess cold gastrin from the binding with labelled gastrin (250 pm) only. at 30 degrees c specific binding rose rapidly to a short-lived maximum before falling gradually, whereas at 15 degrees c and 0 degree c specific binding rose gradually to a higher plateau level. the reduced binding at 30 degrees c could be caused by degradatio ...19863018914
flow cytometric analysis and sorting of live female rat anterior pituitary cell types by forward angle and perpendicular light scatter: effect of 17 beta-estradiol.administration of physiological concentrations of 17 beta-estradiol (e2) to ovariectomized rats resulted in changes in the forward angle light scatter (fals) and perpendicular light scatter (pls) signals of anterior pituitary lobe cells. one day after steroid treatment some cells showed increases in pls signals to an intermediate ridge position lying between the two ridges seen for cells prepared from oil-treated ovariectomized rats. over the first 5 days of e2 treatment, the major effect was a ...19863023018
flow cytometric analysis and sorting of live female rat anterior pituitary cell types by forward angle and perpendicular light scatter: effect of 17 beta-estradiol.administration of physiological concentrations of 17 beta-estradiol (e2) to ovariectomized rats resulted in changes in the forward angle light scatter (fals) and perpendicular light scatter (pls) signals of anterior pituitary lobe cells. one day after steroid treatment some cells showed increases in pls signals to an intermediate ridge position lying between the two ridges seen for cells prepared from oil-treated ovariectomized rats. over the first 5 days of e2 treatment, the major effect was a ...19863023018
septic complications in patients with permanent pacemakers.nine patients with implanted pacemakers had the diagnosis of septicaemia to endocarditis. the diagnosis was established on the basis of a repeatedly positive haemoculture. the interval since the first pacemaker implantation to the onset of sepsis to endocarditis was about 5 years. all nine patients had previous reoperation either of the pacemaker or its lead due to decubitus. while, in four patients, the route of infection was a pacemaker lead in its extravascular couse, in 5 patients the source ...19883069327
the effects of heparin on the physicochemical properties of reconstituted collagen.pepsin-solubilized bovine dermal collagen was reconstituted in 0.02 m sodium phosphate (ph 7.2), concentrated to 30-40 mg/ml, and adjusted to physiological ionic strength by addition of sodium chloride. these preparations, at 4-15 degrees c, are fibrillar suspensions composed of fibrils of varying diameters and nonassociated molecules. addition of heparin to these suspensions promoted a dose-dependent increase in average fibril diameter as measured by turbidimetry and electron microscopic analys ...19883126021
low propensity for poultry isolates of pasteurella multocida to acquire adaptive resistance to oxytetracycline.thirty independently derived reference strains and clinical isolates of pasteurella multocida were tested to determine their potential for acquiring adaptive resistance to oxytetracycline in an effort to better understand the prolonged high efficacy of the antibiotic for pasteurellosis in poultry. all reference strains and clinical isolates exhibited uniform susceptibility as measured with the broth dilution method. none of the strains or isolates readily acquired significant resistance when gro ...19883196263
[kinetics of dna reparative replication in the spleen cells of cba- and c57bl/6-strain mice induced by urethane and and the influenza virus].different kinetics of dna repair replication induced by urethan and influenza virus was detected in mice of varying genotypes. inhibition of repair replication was detected in the lymphocytes of c57bl/6 mice, infected with influenza virus and treated with urethan. no inhibition of repair replication was noted in cba mice which is characteristic of resistance to influenza virus. however, stimulation of repair replication by influenza virus was observed in these cells.19863697490
soft drug derivatives related to perhexiline. part ii: in vivo studies.a series of cyclohexylaralkylamines derived from perhexiline and synthesized according to the "soft drug" concept were evaluated for haemodynamic effects and myocardial oxygen consumption in vivo. in anaesthetized rabbits, the most active compounds, 2, 5, and 11 decreased arterial blood pressure, to a greater extent dp/dtmax and, to a lesser extent heart rate. the decrease in cardiac work and the decrease in cardiac output varied concomitantly. the cardiac effort index decreased in all cases to ...19883196404
temporal coding of resonances by low-frequency auditory nerve fibers: single-fiber responses and a population model.1. we recorded responses of low-frequency auditory nerve fibers (characteristic frequency (cf) less than 3 khz) in the cat to resonant stimuli with varied natural frequencies, damping coefficients, and sound pressure levels. responses to resonances were synchronized to frequencies lying between the peak frequency of the stimulus spectrum and a frequency near the fiber's cf. the frequency of the dominant synchrony in the response varied systematically as a function of the stimulus parameters. 2. ...19883199176
ultrastructural identification of calbindin-d28k-immunoreactive cells in the rat superficial pineal body.the vitamin-d-dependent calcium-binding protein, calbindin-d28k, was detected immunocytochemically in the majority of pericapillary pinealocytes of the rat superficial pineal body. the interstitial cells were calbindin-negative. the significance of these findings is discussed.19883213416
the cardiovascular effects of deconditioning after endurance training in rats.the haemodynamic effects of endurance training and physical deconditioning were studied in anaesthetized rats using aortic and left ventricular pressure recordings and volume measurements by thermodilution method during isoproterenol and cacl2 loads. the resting stroke volume was significantly larger in the training group (tg i, n = 10) than in the control group (cg i, n = 13). during the cacl2 infusion stroke index, end-diastolic and end-systolic volumes increased in the tg i, but decreased in ...19883227925
oxygen toxicity protecting enzymes in the human brain.regional distribution of superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activities were studied in 22 anatomic sites of 5 human brains. no significant regional differences were observed in cytosolic activities of any enzyme studied, nor in particulate activities of superoxide dismutase, catalase and glutathione reductase, whereas particulate glutathione peroxidase activities were distributed unevenly, the highest activities observed in the basal nucleus and amy ...19883235996
hepatitis b virus infection in children in taiwan. 19883273357
hydrophobic interactions of brush border alkaline phosphatases: the role of phosphatidyl inositol.tissue-specific (intestinal) and tissue-nonspecific (kidney) rat alkaline phosphatases are released from their respective brush border membranes by different enzymes. to elucidate the mechanism underlying their membrane attachment, we tested the ability of these enzymes to partition into lipid or aqueous phases both before and after treatment with phospholipases and proteases. interaction with triton x-114 micelles was eliminated or decreased by treatment of intestinal enzyme with phospholipase ...19873813562
human lipoprotein lipase complementary dna sequence.lipoprotein lipase is a key enzyme of lipid metabolism that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins. it has been proposed that the enzyme plays a role in the development of obesity and atherosclerosis. the human enzyme has been difficult to purify and its protein sequence was heretofore undetermined. a complementary dna for human lipoprotein lipase that codes for a mature protein of 448 amino acids has now bee ...19873823907
[non-homogeneity of maturity of cells of the granulocyte line from human bone marrow in in vivo culture in diffusion chambers]. 19853835772
the value of spectrofluorometry for the diagnosis of contagious equine metritis. 19853838846
investigations of the pathogenicity of salmonella enteritidis serotype amsterdam following a naturally occurring infection in rats.a naturally occurring infection of an unusual salmonella sp. was detected in research rats during routine health monitoring procedures. the organism, salmonella enteritidis serotype amsterdam appeared to have a low pathogenicity and did not cause clinical disease in adult rats. experimental mouse and guinea pig inoculations suggest that the organism is more pathogenic in mice.19873302533
nasopharyngeal carcinoma: result of treatment with cis-diamminedichloroplatinum ii, 5 fluorouracil, and radiation therapy.combined ct (cddp + 5fu) and rt were given to 28 patients with npc during july 1982-may 1985. two, 1, 4, and 21 were in stages i-iv (ajc), respectively. none had distant metastasis. four did not complete the planned treatment, and one each had more or fewer ct courses than planned. the median duration of follow-up of surviving patients was 29 months (19-52,x 31.1). objective response (cr + pr) at the primary lesion was 27/28 (96.4%), whereas cr was 23/28 (82%). cr + pr and cr of the regional nod ...19883343153
comparison of cryofixation methods for skin biopsies from sheep infested with psoroptes ovis (acari: psoroptidae).two methods of cryofixing skin samples from sheep infested with psoroptes ovis were compared. in one method the skin biopsy was placed in a capped vial before immersion, while the other allowed direct contact with liquid nitrogen. no statistically significant differences were found between the cryofixation methods with regard to location of p. ovis sections or engaged mouthparts. direct contact with liquid nitrogen is recommended as the preferred method for cryofixing epidermal feeding ectoparas ...19883388743
topographically selective reinnervation of adult mammalian skeletal muscles.in 2 rat muscles, serratus anterior and the diaphragm, the rostrocaudal axis of the motor pool is mapped onto the rostrocaudal axis of the muscle's surface (laskowski and sanes, 1987a). one possible basis for this orderly topography is that motor axons and intramuscular structures bear labels that favor connectivity among positionally matched partners. to test for the existence of such labels, we asked whether axons would selectively reinnervate appropriate portions of the muscles following nerv ...19883411370
catalase-dependent ethanol oxidation in perfused rat liver. requirement for fatty-acid-stimulated h2o2 production by peroxisomes.the purpose of this study was to measure rates of catalase-dependent ethanol uptake and rates of h2o2 generation in perfused rat livers in the presence of fatty acids of varying chain length. rates of ethanol uptake in livers from fasted rats, perfused in a recirculating system, of about 80 mumol g-1 h-1 were decreased to about 10 mumol g-1 h-1 by the addition of an inhibitor of alcohol dehydrogenase (adh), 4-methylpyrazole. the medium-chain-length fatty acid, laurate (12:0; 1 mm), increased rat ...19883416882
abundant nuclear ribonucleoprotein form of cad rna.transcripts of the cad gene in syrian hamster cells are as abundant in the nucleus as in the cytoplasm. this was shown by in situ hybridization of whole cells and by solution and blot hybridization of subcellular fractions. similar results were obtained both for wild-type cells and for a mutant containing amplified cad genes in which the level of cad rna is 150-fold greater. cad nuclear rna is indistinguishable from mature mrna by gel electrophoresis and blot hybridization. discrete higher-molec ...19853990684
abundant nuclear ribonucleoprotein form of cad rna.transcripts of the cad gene in syrian hamster cells are as abundant in the nucleus as in the cytoplasm. this was shown by in situ hybridization of whole cells and by solution and blot hybridization of subcellular fractions. similar results were obtained both for wild-type cells and for a mutant containing amplified cad genes in which the level of cad rna is 150-fold greater. cad nuclear rna is indistinguishable from mature mrna by gel electrophoresis and blot hybridization. discrete higher-molec ...19853990684
[thromboplastic activity of higher plant extracts].hemostatic properties of extracts isolated from the birch floscules and willow blossoms have been revealed. an agent of thromboplastic nature which is identical to thromboplasin of the rat brain according to its biochemical composition and procoagulative action has been found in these extracts. in contrast to brain thromboplastin thromboplastic activity of plant extracts does not possess the species specificity. after intravenous injection of the plant extracts a reaction similar to defence reac ...19854005329
mechanism of ammonium translocation in rat liver mitochondria. finger-printing of the translocator.we have studied whether ammonia crosses the cristae membrane of rat liver mitochondria as a charged (nh4+) or an uncharged (nh3) species. passive swelling of mitochondria suspended in ammonium and/or sodium acetates showed that: swelling depends upon ammonium concentration in the same way it depends on sodium (at constant acetate concentration). the curves reach a plateau at 115 mm ammonium and 100 mm sodium. a two units change of ph induces a two orders of magnitude change in the concentration ...19873425342
neutrophil enzymes, antiproteases, and oxygen radicals: interaction in cartilage proteoglycan degradation and effect of d-penicillamine. 19863460315
effect of acth, beta-endorphin, morphine and naloxone on the release of cortisol by isolated adrenal glands.the release of cortisol (determined by ria) from isolated slices of adrenal glands of guinea pigs is stimulated by acth, by beta-endorphin, and by morphine in a concentration-dependent way; naloxone gives a small stimulation which is not related to its concentration. naloxone inhibits the effect of acth (1.11 x 10(-11) m) in a competitive manner with an ic50 of about 3.10(-9) m. also morphine and beta-endorphin inhibit the effect of acth, but not in competitive manner. naloxone (10(-9)-10(-7) m) ...19852997810
[aminoglutethimide-induced dyslipidemia. experimental study].aminoglutethimide (ag) is a drug that inhibits steroid synthesis; it is used in advanced breast cancer. after the observation of abnormalities in lipid metabolism in patients, we realized an experimental study to try to look for a pathogenetic hypothesis. three groups of rats received respectively ag, hydrocortisone (hc) or both (ag + hc) and were compared to controls. animals treated with ag (with or without hc) had a greater liver content of triglycerides, cholesterol and phospholipids than co ...19854063538
dna filter retention assay for exonuclease activities. application to the analysis of processivity of phage t5 induced 5'-exonuclease.the 5'-exonuclease of phage t5 has been purified nearly to homogeneity by using a simple and fast procedure. the kinetic properties of the purified enzyme have been studied by using a new sensitive assay based upon retention by nitrocellulose filters of dna with short protruding single-stranded ends. the enzyme is specifically stimulated by kcl. its km is 2.2 x 10(-7) m at 30 degrees c, and its turnover number is 0.33 dna molecule transformed per minute. the filter retention assay shows that the ...19853004572
clarifying electron micrograph labeling. 19863012129
a novel yeast mutant defective in the processing of ras proteins: assessment of the effect of the mutation on processing steps.biosynthesis of ras1 and ras2 proteins of saccharomyces cerevisiae involves processing, fatty acid acylation and transport to plasma membranes. we now report the isolation of a mutant, termed dpr1, defective in these biosynthetic events. the dpr1 cells are temperature sensitive for growth and display sterile phenotype specific to a cells. the following observations were made using cells overproducing the ras2 protein. (i) in the dpr1 cells, the ras2 proteins remain as precursors and accumulate i ...19873556161
cimetidine and neonatal small bowel adaptation: an experimental study.histamine2-blockers are being used more extensively in the pediatric short gut patient as an agent to accelerate small bowel adaptation. reversal of lipid malabsorption and a direct trophic effect on the intestinal crypt cells have been postulated as the mechanism for the salutary influence of cimetidine. weanling sprague-dawley rats underwent 85% small bowel resection. controls had repair of a simple ileal transection. rats received either a fat-defined rat chow only, or chow with high- or low- ...19873612436
picomole analysis of glutathione, glutathione disulfide, glutathione s-sulfonate, and cysteine s-sulfonate by high-performance liquid chromatography.a method for simultaneous detection of picomole quantities of glutathione (gsh), glutathione disulfide (gssg), glutathione s-sulfonate (gsso3h), and cysteine s-sulfonate (cysso3h) by high-performance liquid chromatography has been developed. compounds are separated by anion-exchange chromatography using a citric acid buffer system, and then derivatized postcolumn using o-phthalaldehyde with 2-mercaptoethanol, heated to 70 degrees c, and detected by fluorescence. the compounds elute with retentio ...19854096380
the identification of fractions enriched in nonmyelinated axons from rat whole brain. 19704099991
[utilization of amino acids by various types of staphylococci and micrococci]. 19714108718
female endocrine control mechanisms during the neonatal period. ii. the effect of luteinizing hormone. 19724117571
oxytocin and vasopressin immunoreactivity in hypothalamic and extrahypothalamic sites in late pregnant and postpartum rats.this study examines immunoreactive levels of oxytocin (ot) and arginine-vasopressin (avp) from acid extracts of the paraventricular nucleus (pvn), supraoptic nucleus (son), anterior commissural nucleus (acn) and the suprachiasmatic nucleus (scn) as well as selected extrahypothalamic sites in pregnant or postpartum (pp) rats. animals are sacrificed between 08.30 and 10.30 h 16 or 22 days after sperm is detected in their vaginal smears or on the morning after parturition. peptide levels of pregnan ...19873614554
[the effect of mitomycin c, actinomycin c and puromycin on the synthesis of protein in cell strains obtained from human tumors]. 19654163025
the effect of herbicides on rhizobium. 3. influence of herbicides on mutation. 19684172273
iron metabolism by reticuloendothelial cells in vitro. physical and chemical conditions, lipotrope deficiency, and acute inflammation. 19694185856
antigenic differences between isolates of swine vesicular disease virus and their relationship to coxsackie b5 virus. 19734202480
nutritional status of children at namitambo, malawi. 19724203474
proceedings: differential media for the isolation of listeria monocytogenes. 19724206777
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