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application of histochemical and histological stains to epoxy sections: pre-treatment with potassium permanganate and oxalic acid. 197884320
antigenic relationships between the typhus and spotted fever groups of rickettsiae.paired sera from cases of epidemic typhus in ethiopia and from probable cases of rocky mountain spotted fever (rmsf) in the united states were examined by microagglutination (ma) and microimmunofluorescence (micro-if) tests for antibodies against rickettsia prowazekii, rickettsia typhi, rickettsia canada, rickettsia rickettsii, rickettsia conorii and ricksettsia akari. igg and igm antibodies against the various rickettsiae were titrated with specific fluorescein-conjugated anti-igg and anti-igm ...197899029
[action of the lysozyme isolated from ixodid tick species on rickettsia prowazekii, rickettsia canada and rickettsia sibirica]. 1979117293
antigenic relations of rickettsia prowazekii and rickettsia canada, established in the study of sera of patients with brill's disease.sera of patients with brill's disease and of healthy persons with spotted fever in their past history were examined in the complement fixation reaction (cfr) to determine antigenic relations between r. prowazekii and r. canada. r. canada was found to have common antigenic determinants with r. prowazekii and r. mooseri. however, the antigenic determinants of r. canada differed from those of the mentioned rickettsiae. the titres of complement-fixing antibodies in the sera of patients with brill's ...1977560410
experimental forms of infection and serological analysis of the antigenic structure of rickettsia canada.experimental forms of rickettsia canada infection were characterized and serological analysis of the antigenic structure of r. canada was carried out. according to its pathogenicity for experimental animals, r. canada can be characterized as a poorly virulent species of rickettsiae, similar to r. prowazekii (for guinea pigs). the complement-fixing, haemagglutinating and agglutinating antigens of r. canada are fairly similar to those of the typhus group rickettsiae. the region of antigenic activi ...1977580434
antigenic relationships among the rickettsiae of the spotted fever and typhus groups.using immunoblots to analyze antigenic relationships among the pathogenic spotted fever and typhus group rickettsiae, i found that the rickettsial lipopolysaccharide (lps) was a group-specific antigen. all the rickettsiae examined had 135-kda and 58-kda protein antigens. the spotted fever rickettsiae and rickettsia canada had, in addition, 190-kda protein antigens which were antigenic analogs of previously described protective antigens of r. conorii and r. rickettsii.19911916232
effects of amino acid infusion on renal hemodynamics. role of endothelium-derived relaxing factor.ingestion of protein or intravenous infusion of amino acids acutely elevates glomerular filtration rate (gfr) and renal plasma flow (rpf) by unknown mechanisms. endothelium-derived relaxing factor (edrf), now known to be nitric oxide derived from metabolism of l-arginine, participates in local regulation of vascular tone. to investigate the hypothesis that edrf may participate in the renal vasodilatation and increased gfr after amino acid infusion, we characterized the effect of inhibition of ed ...19912045148
heterogeneity of cd4-positive human t-cell clones which recognize the surface protein antigen of rickettsia typhi.immunity to the typhus group of rickettsiae is largely dependent on the effector function of several classes of t lymphocytes, including those which produce gamma interferon. since the surface protein antigen (spa) derived from typhus group rickettsiae has been shown to be an effective immunogen in animal models, human t-cell clones specific for the spa of rickettsia typhi were isolated and tested for their antigenic specificity, as well as for their ability to produce gamma interferon. eighteen ...19892466795
human aldehyde dehydrogenase: coenzyme binding studies.the binding of nadh and nad+ to the human liver cytoplasmic, e1, and mitochondrial, e2, isozymes at ph 7.0 and 25 degrees c was studied by the nadh fluorescence enhancement technique, the sedimentation technique, and steady-state kinetics. the binding of radiolabeled [14c]nadh and [14c]nad+ to the e1 isozyme when measured by the sedimentation technique yielded linear scatchard plots with a dissociation constant of 17.6 microm for nadh and 21.4 microm for nad+ and a stoichiometry of ca. two coenz ...19892775711
characterization of mannoproteins from a virulent candida albicans strain and its derived, avirulent strain.antigenic differences between a wild-type virulent strain of candida albicans 4918 (wt) and its spontaneous avirulent mutant (m-10) were found with crossed-immunoelectrophoresis and western blotting. mannoprotein fractions from yeast and mycelial forms were obtained by affinity chromatography on concanavalin a and analyzed with use of patient sera or sera from rabbits infected with live wt cells and boosted with whole extracts or rabbits immunized with purified wt cell wall preparations. a spike ...19883055210
[experimental chemo- and chemoendocrine therapy of human breast carcinomas serially transplanted into nude mice].four human breast carcinoma strains serially transplanted into nude mice were used for the experimental chemotherapy and combination chemoendocrine therapy. whereas three of these strains (mcf-7, br-10, tm-61) possessed cytosol estrogen receptor (erc) and were dependent on estradiol for the tumor growth, the other strain (mx-1) without erc was hormone independent. for the chemotherapy, mitomycin c (mmc), adriamycin (adm), cyclophosphamide (cpm) and 5-fluorouracil (5-fu) were administered 3 times ...19883129645
endoscopic findings of bleeding esophageal varices and experimental study.it has been considered that the rupture of esophageal varices occurs with high incidences, and once bleeding occurs it sometimes cause fetal massive bleeding. however, from our experiences with the active application of emergency endoscopy, the incidence of bleeding from varix was unexpectedly low. from these results, we studied the rupture signs of esophageal varices in experiments on dogs and clinical cases. the actual rupture signs of varices were summarized as active bleeding, adhesion of fr ...19873497471
perturbation of differentiated functions in vivo during persistent viral infection. iii. decreased growth hormone mrna.lymphocytic choriomeningitis virus (lcmv) persistent infection that results from the inoculation of c3h/st newborn mice causes growth hormone (gh) deficiency and associated disease characterized by both reduced weight and serum glucose levels. molecular analysis of pituitary nucleic acids shows gh deficient mice have, on average, fivefold reduced levels of gh mrna although the histopathology of such gh producing cells is normal. northern blots indicate that the length of gh mrna is comparable in ...19873811236
zoonotic infections in military scout and tracker dogs in vietnam.significant levels of antibodies indicative of a variety of zoonotic infections were demonstrated in sera collected from 64 u.s. military scout and tracker dogs after service in the republic of vietnam (rvn). scrub typhus antibodies were found in 45% of the specimens, melioidosis in 19%, group b arbovirus in 49%, and leptospirosis in 62%. only 38% of the seropositive reactions for leptospirosis could be related to overseas infections. tests were conducted on paired sera obtained from 32 of the d ...19724564881
[electron microscope study of rickettsia canada in tissue culture cells]. 19734576056
serologic evidence of rickettsia canada infection of man. 19704985949
genotype characterization of the bacterium expressing the male-killing trait in the ladybird beetle adalia bipunctata with specific rickettsial molecular tools.the male-killing ladybird beetle (lb) bacterium (ab bacterium) was analyzed with specific rickettsial molecular biology tools in the lb adalia bipunctata strains. eight phenotype-positive lb strains showing mortality of male embryos were amplified with rickettsial genus-specific primers from the gene for citrate synthase (cs) and the gene for a 17-kda protein and spotted fever group-specific primers from the gene for the 120-kda outer membrane protein (ompb). the specificity of amplification was ...19957747963
citrate synthase gene comparison, a new tool for phylogenetic analysis, and its application for the rickettsiae.using pcr and an automated laser fluorescent dna sequencer, we amplified and sequenced a 1,234-bp fragment of the citrate synthase-encoding gene (glta) of 28 bacteria belonging to the genus rickettsia. comparative sequence analysis showed that most of the spotted fever group (sfg) rickettsiae belonged to one of two subgroups. the first subgroup included rickettsia massiliae, strain bar 29, rickettsia rhipicephali, "rickettsia aeschlimanni," and rickettsia montana, which have been isolated only f ...19979103608
molecular phylogeny and rearrangement of rrna genes in rickettsia species.it has previously been observed that rickettsia prowazekii has an unusual arrangement of the rrna genes. in this species, the three rrna genes, 16s (rrs), 23s (rrl), and 5s (rrf), are not linked in the typical arrangements for bacteria. rather, the 16s rrna gene has been separated from the 23s and 5s rrna gene cluster, and the 23s rrna gene is preceded by a gene which codes for methionyl-trnaf(met) formyltransferase (fmt). in this study, we screened the genus rickettsia for the fmt-rrl motif in ...199910406115
phylogenetic analysis of members of the genus rickettsia using the gene encoding the outer-membrane protein rompb (ompb).to confirm the phylogenetic analysis previously inferred by comparison of the citrate synthase and rompa gene sequences (gita and ompa, respectively), the rompb gene (ompb) of 24 strains of the genus rickettsia was amplified and sequenced. rompb is an outer-membrane protein of high molecular mass, the presence of which can be demonstrated in most rickettsiae by immunological cross-reactivity in western blots. no pcr amplification was obtained with rickettsia bellii or rickettsia canadensis. for ...200010939649
intranuclear growth of rickettsia canada, a member of the typhus group.intranuclear development of rickettsia canada, a typhus-group rickettsia, in cells of tick tissues is described and illustrated.197016557789
[primary transport of acute patients. optimal use of rescue resources exemplified by the canton of graubünden]. 20113927607
when is serum albumin worth measuring? 20113214132
child abuse: does prosecution serve any useful purpose? 20061635513
ovarian carcinoma: abdominopelvic irradiation following reexploration.twenty patients with ovarian cancer, who following reexploration were left with residual disease nodules of less than 2 cm diameter, received abdominopelvic irradiation. of these patients 18 had previous chemotherapy. fifteen patients completed treatment, 13 of whom had prior chemotherapy. six of the 18 patients with invasive tumors were alive and disease free 18-53 months postradiotherapy, while only 4 patients had died at the time of follow-up. no patient with residual nodules greater than 1 c ...20113141249
hypophosphatemia-associated respiratory muscle weakness in a general inpatient population.although hypophosphatemia has been implicated as a cause of respiratory failure, its impact on respiratory muscle function in patients hospitalized for other reasons remains to be determined. maximal inspiratory pressures (mip) and maximal expiratory pressures (mep) were measured at the bedside in 23 hospitalized patients with serum phosphate levels less than 2.5 mg/dl, and these measurements were repeated daily during phosphate repletion until serum phosphate levels reached the normal range. a ...20123364446
[measurement of orthodontic appliance force using prototype sensors].in introductory remarks the most important biomechanical principles, which are relevant for the research, and conditions which must be satisfied presented in intraoral measuring of orthodontic forces were pointed out. first the aim of the research was defined, and then the investigation of mechanical properties of acrylics as a material for orthodontic appliances were carried out. the type of strain gauges, bonds and measuring system configurations, as well as measuring amplifiers and recording ...20082635572
oral biopsy technique. the pathologist's perspective. 20123524767
[microbiological findings in patients with kidney transplantations]. 20164915085
[stereotaxic procedures of the thalamus in painful conditions]. 20164899400
risk factors for infantile recurrent otitis media: atopy but not type of feeding.we followed 183 infants from birth to 2.3 yr of age. of these infants 28 had recurrent otitis media (rom), defined as five or more separate episodes of otitis media (om) during the first 2 yr of life or four such episodes during their 2nd yr. the om presented during their 1st yr (early-onset rom) in 12 infants and during their 2nd yr (2nd yr rom) in 16. eighty infants had no om and served as a comparison group. regarding type of feeding, the infants with early-onset rom did not differ from their ...20123387173
the hp, gc, gm, km and beta 2-glycoprotein i genetic variants in hairy cell leukemia: no apparent association.no significant differences could be observed when hp, gc, gm, km and beta 2-glycoprotein i phenotypes of 50 patients with hairy cell leukemia were compared with healthy unrelated adults.20133923375
history of medicine in alaska. harriet crocker jackson schirmer, m.d. 20092662801
separation and identification of trifluralin metabolites by open-tubular liquid chromatography/negative chemical ionization mass spectrometry.the utility of combined open-tubular liquid chromatography/negative ion chemical ionization (nci) mass spectrometry for the separation and analysis of trifluralin, a commonly used pre-emergence herbicide, and seven of its metabolites has been demonstrated. the nci mass spectra for these compounds are reported. some of the nci mass spectra were obtained from as little as 50 pg of analyte.20113203148
the effects of general anesthesia on upper respiratory tract infections in children.a prospective cohort study of 489 pediatric patients was performed to investigate the prevalence of perioperative respiratory complications and symptomatology in children presenting for myringotomy with upper respiratory tract infections (uris). all children undergoing myringotomy received halothane n2o/o2 anesthesia administered via face mask. information on complications and respiratory symptoms was obtained from the anesthesia and recovery room records, and by standardized questionnaire. ther ...20133688537
focal dermal hypoplasia syndrome (goltz syndrome): the first dental case report. 20123466146
[case of ovarian thecoma]. 20154350562
[transketolase activity as a parameter for the vitamin b1 provision in some dermatoses (herpes zoster, psoriasis, drug eruptions, excema, and urticaria) (author's transl)]. 2003153861
provision of a lingual biting platform in a severe class iii malocclusion: a case report. 2004321730
long-term enteral feeding: a retrospective comparison of delivery via percutaneous endoscopic gastrostomy and nasoenteric tubes.the use of percutaneous endoscopic gastrostomy (peg) tubes for enteral feeding is widespread, although their superiority to other feeding devices, such as nasoenteric tubes (net), has not been substantiated. we retrospectively compared clinical outcomes in patients who received enteral feeding via peg (n = 80) or net (n = 29) from 1984 to 1988. mean follow-up was 192 days in the peg group and 141 days in the net group. changes in nutritional and performance status were similar in both groups. as ...20071951237
report of the second public health service aids prevention and control conference. women and aids. 20113147491
a medical center-based tertiary reference laboratory. experience at mayo medical laboratories (mml). 20123415449
"where" and "what" in vision.the mixture of a few horizontal and vertical line segments embedded in an aggregate of diagonal line segments can be rapidly counted and their positions rapidly determined by a parallel (preattentive) process. however, the discrimination between horizontal and vertical orientation (that is, discrimination of a single conspicuous feature) requires serial search by focal attention. under recent theories of attention, focal attention has been assumed to be required for the recognition of different ...20124001937
rickettsia infection in amblyomma tonelliae, a tick species from the amblyomma cajennense complex.the present study was performed to evaluate the rickettsia infection in amblyomma tonelliae ticks from argentina. all ticks were subjected to dna extraction and tested by a battery of pcrs to amplify fragments of four rickettsial genes, 23s-5s, glta, ompa and htra. two ticks were positive. the rickettsia detected in one tick represents a new lineage which is named rickettsia sp. strain el tunal. this new strain belongs to the canadensis group because it is closely related to rickettsia monteiroi ...201525544308
[treatment of breastplate deformities: assessment using ct scanning and functional tests].a study to evaluate both the pre- en postoperative functional and morphologic status of major anterior chest wall deformities. four of the eight patients suffered from a progressive reduction in physical capabilities. preoperative pulmonary function tests demonstrated restriction in pulmonary function. in the postoperative period there was progressive return to normal physical strength without objective improvement in pulmonary function. pre- and postoperative thoracometric study by ct scan allo ...20071950309
cefamandole levels in serum and necrotic bone.seven patients with chronic osteomyelitis were treated by surgical debridement. cefamandole was administered intravenously before surgery. during the debridement, cefamandole concentrations were measured in serum and necrotic bone. although adequate levels of antibiotic were achieved in the serum, minimal or no concentration of antibiotic was found in the necrotic bone. there was only minimal penetration of cefamandole into necrotic bone.20144042490
rickettsia monteiroi sp. nov., infecting the tick amblyomma incisum in brazil.free-living adult amblyomma incisum ticks were collected in an atlantic rainforest area at intervales state park, state of s+úo paulo, brazil. from an a. incisum specimen, rickettsiae were successfully isolated in vero cell culture by the shell vial technique. rickettsial isolation was confirmed by optical microscopy, transmission electron microscopy, and pcrs targeting portions of the rickettsial genes glta, htra, rrs, and sca1 on infected cells. fragments of 1,089, 457, 1,362, and 443 nucleoti ...201121685169
mitochondrial porin vdac 1 seems to be functional in rickettsial cells.we have recently shown that rickettsia prowazekii (typhus group rickettsiae) cells incorporate human mitochondrial porin vdac1. here, i report on the import of porin by rickettsiae of spotted fever group. it was shown that rickettsial cells of heavy band of renografin density gradient, known as permeabilized rickettsiae, contain much more vdac 1 compared to the cells of light band, that is, non-permeabilized rickettsiae. these data hint at a functionality of mitochondrial porin in rickettsial ce ...200919538262
detection of rickettsial dna in ixodid ticks recovered from dogs and cats in japan.dna from ticks recovered from 1137 dogs and 133 cats from all over japan were examined for rickettsia infection by citrate synthase gene (glta)-based pcr and partial nucleotide sequencing. a total of 91 dog tick samples and 18 cat tick samples showed a single band of the appropriate size in the nested pcr. sequence analysis was successfully performed on 102 samples. dna of rickettsia japonica or closely related rickettsia spp. strains were detected from 38 ticks in 16 prefectures mainly in weste ...200516397379
serologic and molecular evidence of coinfection with multiple vector-borne pathogens in dogs from thailand.forty-nine dogs from thailand were evaluated for serologic evidence of exposure or polymerase chain reaction (pcr) evidence of infection with vectorborne pathogens, including ehrlichia sp. (ehrlichia canis, ehrlichia chaffeensis, ehrlichia equi, and ehrlichia risticii), bartonella vinsonii subsp. berkhoffi (bvb), spotted fever group (sfg) rickettsiae (rickettsia rickettsii), typhus group (tg) rickettsiae (rickettsia canada, rickettsia prowazekii, and rickettsia typhi), and babesia sp. (babesia c ...200111596732
recommended conservation of the names streptococcus sanguis, streptococcus rattus, streptococcus cricetus, and seven other names included in the approved lists of bacterial names. request for an opinion.with reference to the first principle of the international code of nomenclature of bacteria, which emphasizes stability of names, it is proposed that the original names streptococcus sanguis, streptococcus rattus, streptococcus cricetus, erwinia ananas, eubacterium tarantellus, lactobacillus sake, nitrosococcus oceanus, pseudomonas betle, rickettsia canada and streptomyces rangoon, all included in the approved lists of bacterial names, be conserved. request for an opinion.200111321121
evaluation of the pathogenic potential of rickettsia canada and rickettsia prowazekii organisms in dogs.an unusual pattern of seroreactivity to antigens of rickettsial organisms (rickettsia rickettsii, r rhipicephali, r montana, and r bellii), particularly to r bellii antigen, was detected in 3 dogs during a 2-month period. thus, studies were initiated to clarify the pathogenic potential of the more distantly related rickettsial organisms (r canada and r prowazekii) in dogs. because r bellii are nonpathogenic rickettsiae that share numerous common properties with spotted fever-group and typhus-gro ...19957601694
identification of an isolate of rickettsia canada from california.a strain of rickettsia canada was recovered in 1980 an adult rabbit tick, haemaphysalis leporispalustris, taken from a black-tailed jack rabbit, lepus californicus, in mendocino county, california. in all examined biologic characteristics, this isolate, ca410, is indistinguishable from the prototype, strain 2678, isolated in ontario, canada, in 1963. these similarities include serologic and immunologic reactivity in laboratory mice and guinea pigs, cultural characteristics in vero cells, chick e ...19826756179
rickettsia canada: a new member of the typhus group of rickettsiae isolated from haemaphysalis leporispalustris ticks in canada. 19676039198
observations on rickettsia canada a recently described member of the typhus group rickettsiae. 19684975527
significance of genital mycoplasmas. 19734714870
ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase in rat and bovine brain tissues.inositol 1,4,5-trisphosphate (ins p3) 3-kinase catalyzes the atp-dependent phosphorylation of ins p3 to inositol 1,3,4,5-tetrakisphosphate (ins p4). ca2+/calmodulin (cam)-sensitivity of ins p3 3-kinase was measured in the crude soluble fraction from rat brain and different anatomic regions of bovine brain. kinase activity was inhibited in the presence of egta (free ca2+ below 1 nm) as compared to ca2+ (10 microm free ca2+) or ca2+ (10 microm free ca2+) and cam (1 microm). ca2+-sensitivity was al ...19882838022
acute febrile cerebrovasculitis. a non-spotted fever group rickettsial disease.in december 1985, a patient was seen with an illness that was clinically compatible with the recently described clinical syndrome of acute febrile cerebrovasculitis, including fever, headache, altered mentation, multifocal neurologic signs, and cerebrospinal fluid pleocytosis. an extensive medical evaluation failed to reveal a cause, until, retrospectively, she was shown to have antibodies to rickettsia typhi. detailed serologic analysis with enzyme immunoassays and protein immunoblots indicated ...19892742443
characterization of the natural killer cell activity in hashimoto's and graves' diseases.natural killer (nk) cell activity and blood mononuclear cell subpopulations were characterized in patients with hashimoto's thyroiditis (n = 11), graves' disease (n = 20), non-toxic goitre (n = 10) and in normal controls (n = 22). nk cell activity against k 562 target cells and the capability of ifn-alpha, il-2, and indomethacin to enhance nk cell activity in vitro did not differ significantly between the groups. the percentages of large granular lymphocytes, cd5+, cd4+, cd8+ and cd16+ cells wer ...20092817303
recent experience with the complement fixation test in the laboratory diagnosis of rickettsial diseases in the united states.sera from patients suspected of having rickettsial infections were tested in the complement fixation test with antigens prepared from the rickettsiae of rocky mountain spotted fever (sf), rickettsial pox (rp), murine typhus, epidemic typhus, and from rickettsia canada (rc). eight units of antigen were used in all cases and two units in man. only those patients with antibody titers of 1:16 or higher were included in the study. largely on the basis of comparative titers, the patients were divided ...1976972194
injury to the glenoid fossa.an illustrated case of fracture through the confines of the glenoid fossa by the mandibular condyle is presented. the mechanism of injury and postinjury sequelae of this rarely reported but frequently suspected occurrence are discussed and a treatment rationale proposed. the key to the diagnosis of this entity is an awareness of vector forces directed to the region of the glenoid fossa with or without evidence of subcondylar fracture.20143945692
erythrocyte-sensitizing substance from rickettsia canadia.erythrocyte-sensitizing substance (ess) extracted from rickettsia canada reacted with homologous sera as well as with sera containing antibodies against rickettsia prowazeki, but did not react with sera containing antibodies against rickettsia conori or coxiella burneti. this finding confirms the antigenic relatedness of r. canada to the typhus rickettsiae group.1975239581
fine structure of rickettsia canada in tissues of dermacentor andersoni stiles.preliminary observations on growth and developmental fine structure of rickettsia canada in various organs and tissues of the hard tick, dermacentor andersoni stiles, are reported. r. canada is typically rod-shaped, being delimited with a three-layered wall having a velvety coating adsorbed to its exterior surface. a finely reticulated cytoplasmic matrix containing prominent ribosomes is delimited with a three-layered unit membrane. average length and width of these organisms are 1.6 by 0.4 mum. ...19714100833
[interrelationship of ixodoidea ticks and rickettsia canada]. 1976947273
restriction fragment length polymorphism of the dna of typhus group rickettsiae.the dna of 10 strains of rickettsia prowazekii, 5 strains of rickettsia typhi and 1 strain of rickettsia canada was investigated by restriction fragment length polymorphism analysis. interspecies differences were characterized by a great number of noncomigrating bands. using the endonuclease hindiii and psti fragments comigration as a quantitative criterion, genetic similarity coefficient was calculated for the pair rickettsia prowazekii/rickettsia typhi-32.0%, for rickettsia prowazekii/ricketts ...19911687635
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