| tick-borne diseases in the united states: rocky mountain spotted fever and colorado tick fever. a review. | the historical, clinical, ecological, and epidemiological features of rocky mountain spotted fever and colorado tick fever, the two important tick-borne diseases in the united states, are reviewed. rocky mountain spotted fever, once considered a disease of the past, has again become a measurable public health problem. its nationwide incidence has steadily increased since 1960 and has reached record proportions in 1976. the various factors responsible for this trend as well as for the mortality r ... | 1977 | 19954 |
| massive occurrence of rickettsiae of the spotted fever group in fowl tampan, argas persicus, in the armenian s.s.r. | fowl tampans (argas persicus) collected in oktemberyan in the armenian s.s.r. in 1974 were found to be massively infected with rickettsiae of the spotted fever (sf) group. one isolated rickettsial strain, designated armenia 9, is antigenically related to, and probably identical with, strain armenia 11 and strain b of rickettsia slovaca, isolated from dermacentor marginatus ticks. strain armenia 9 grows readily in chick embryo yolk sacs and in chick embryo cell (cec) and l-cell cultures. it cause ... | 1977 | 22239 |
| proteolytic enzymes of the k-1 strain of streptomyces griseus obtained from a commercial preparation (pronase). effect of ph, metal ions, and amino acids on aminopeptidase activity. | | 1978 | 22544 |
| effect of quantity and quality of igg antibodies on blocking of allergenic histamine release in vitro. | in a study of the protective role of igg antibodies in allergen injection therapy, serum antibodies to timothy grass allergens were raised in rabbits and tested for inhibition of histamine release from basophils of grass pollen-sensitive human subjects. rabbit sera were not inhibitory until late in the immune response when large amounts of relatively low affinity igg antibodies were detected by primary binding. inhibition of histamine release by late sera required preincubation of allergen and a ... | 1979 | 84798 |
| expression in escherichia coli of chemically synthesized genes for human insulin. | synthetic genes for human insulin a and b chains were cloned separately in plasmid pbr322. the cloned synthetic genes were then fused to an escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. the insulin peptides were cleaved from beta-galactosidase, detected by radioimmunoassay, and purified. complete purification of the a chain and partial purification of the b chain were achieved. these products were mixed, reduced, and r ... | 1979 | 85300 |
| systemic hyalinosis (juvenile hyaline fibromatosis). ultrastructure of the hyaline with particular reference to the cross-banded structure. | systematic hyalinosis (juvenile hyaline fibromatosis) is characterized by hyalinized skin lesions. electron microscopic examination of a hyalinized skin tumor from a 19-year-old man with this syndrome revealed that the hyaline was composed of ruthenium red-positive ultrastructures (granules, filaments, and a kind of cross-banded structure), indicating the presence of glycosamino-glycan or glycoprotein, and a small number of thin collagen fibrils. using a new ruthenium red staining method combine ... | 1979 | 88923 |
| inhibition of spontaneous transformation of rat embryo cells releasing endogenous type c virus by virus-specific antiserum. | noninbred sprague-dawley rat embryo cell clones predictably undergo transformation after 20-30 in vitro passages following spontaneous release of endogenous rat leukemia virus (ralv). in the presence of ralv-specific antiserum, virus production and infectivity were reduced and transformation was delayed from 6 to 25 weeks. transformation was not associated with an increased expression of kirsten murine sarcoma virus-related src gene rna. | 1979 | 89211 |
| verification of human fetal breathing with phased array ultrasound imaging. | continuous observation of fetal movement patterns is a novel and useful capability of new high-speed ultrasound imaging systems. confirmatory documentation of human fetal breathing as a component of these movement patterns has been achieved using a phased-array real time ultrasound sector scanning system. this system permits direct visualization of fetal breathing movements. permanent recordings can be made on videotape. recordings can also be made as a time-motion echocardiograms during continu ... | 1978 | 96144 |
| regulation of the in vitro synthesis of the alpha-peptide of beta-galactosidase directed by a restriction fragment of the lactose operon. | | 1978 | 96823 |
| effect of immunosuppression on rickettsia rickettsii infection in guinea pigs. | the role of the immune response in the pathogenesis of rickettsia rickettsii infection in guinea pigs was investigated by immunosuppression, using antilymphocyte serum. twenty guinea pigs were inoculated with r. rickettsii, sheila smith strain, on day 0. fifteen animals received antilymphocyte serum on days --1, 0, 2, 4, and 6. five animals received normal rabbit serum on the same schedule. at necropsy, specimens were collected for histological examination, rickettsial immunofluorescence, ricket ... | 1978 | 97223 |
| detection of the rocky mountain spotted fever agent, rickettsia rickettsii, in dead ticks, dermacentor andersoni. | rickettsia rickettsii remained viable and retained chromophilic properties for not more than 24 h after dermacentor andersoni were killed by freezing. antigenic reactivity was detected for at least 71 days by direct immunofluorescence. however, rickettsiae in ticks suffocated with mineral oil remained pathogenic for at least 14 days. accordingly, ticks removed from a host by mineral oil or dying from desiccation in transit are still suitable for rickettsial examination. | 1978 | 97233 |
| an improved electrophoretic method for identifying antibiotics with special reference to animal tissues and animal feeding stuffs. | | 1978 | 97257 |
| prophylactic treatment of rocky mountain spotted fever. | prophylactic treatment of rocky mountain spotted fever with a single dose of oxytetracycline was investigated in guinea pigs. disease was prevented when treatment was administered shortly before expected onset. relapses occurred when treatment preceded expected onset by 48 h or more. | 1978 | 97307 |
| rickettsiae: a new vaccine for rocky mountain spotted fever. | | 1978 | 97783 |
| antigenic relationships between the typhus and spotted fever groups of rickettsiae. | paired sera from cases of epidemic typhus in ethiopia and from probable cases of rocky mountain spotted fever (rmsf) in the united states were examined by microagglutination (ma) and microimmunofluorescence (micro-if) tests for antibodies against rickettsia prowazekii, rickettsia typhi, rickettsia canada, rickettsia rickettsii, rickettsia conorii and ricksettsia akari. igg and igm antibodies against the various rickettsiae were titrated with specific fluorescein-conjugated anti-igg and anti-igm ... | 1978 | 99029 |
| identification of rickettsia rickettsii in formalin-fixed, paraffin-embedded tissues by immunofluorescence. | with slight modification of a trypsin digestion technique, rickettsia rickettsii were demonstrated specifically by immunofluorescence staining in formalin-fixed, paraffin-embedded tissue sections from a human, rhesus monkey, and guinea pig with rocky mountain spotted fever and in infected membranes from a chicken embryo. tissues were cut at 4 micron and, using geltain as a tissue adhesive, were hydrated in a routine manner. sections were then digested in refrigerated 0.1% trypsin for 16 h, washe ... | 1978 | 100509 |
| serologic typing of rickettsiae of the spotted fever group by microimmunofluorescence. | | 1978 | 101593 |
| distribution and prevalence of spotted fever group rickettsiae in ticks from south carolina, with an epidemiological survey of persons bitten by infected ticks. | live ticks removed from human hosts were tested for the presence of spotted fever group rickettsiae from 1974 through 1976. spotted fever group rickettsiae were detected in dermacentor variabilis, amblyomma americanum, amblyomma maculatum, and ixodes scapularis. persons from whom positive ticks were removed in 1974 and 1975 were questioned about ensuing illness. eleven of 51 persons from whom rickettsia-positive d. variabilis were removed reported some unexplained combination of symptoms compati ... | 1978 | 103448 |
| growth characteristics of atypical mycobacteria in water and their comparative resistance to disinfectants. | with the increasing significance of group iv atypical mycobacteria as etiological agents in a variety of infections, studies were conducted to determine their growth capabilities in water and their comparative resistance to disinfectants used to decontaminate hospital equipment. isolates of mycobaterium chelonei (tm strains) from peritoneal fluids of patients and peritoneal dialysis machines were able to multiply in commercial distilled water, with generation times at 25 degrees c ranging from 8 ... | 1978 | 104656 |
| increased levels of dihydrofolate reductase in rifampin-resistant mutants of bacillus subtilis. | several independent, spontaneous rifampin-resistant mutants of bacillus subtilis were isolated and found to have an increased resistance to trimethoprim, an inhibitor of dihydrofolate reductase. this increased resistance in the rif mutants was the result of a specific threefold increase in the activity of dihydrofolate reductase, since six other enzymes examined remained unchanged. this increased level of dihydrofolate reductase and the trimethoprim resistance were cotransformed (100%) with the ... | 1979 | 106041 |
| transfer of 2,4,5,2',4',5'-hexachlorobiphenyl and 2,2-bis-(p-chlorophenyl),1,1,1-trichloroethane (p,p'-ddt) from maternal to newborn and suckling rats. | pregnant rats were given a small dose of 14c-2,4,5,2',4',5'-hexachlorobiphenyl (hcb) and 3h-ddt intraperitoneally. the transfer of hcb and ddt through the placenta and milk was then investigated. transfer through the placenta was 2.7 and 1.5% (respectively) of the initial doses; transfer through milk was 39.2 and 21.5%. hcb is obviously more transferable than ddt through the placenta and milk, the ratio of the amount of hcb transferred through milk to the amount transferred through the placenta ... | 1978 | 104695 |
| comparative ultrastructural study on the cell envelopes of rickettsia prowazekii, rickettsia rickettsii, and rickettsia tsutsugamushi. | rickettsia tsutsugamushi differs from other rickettsiae in its cell envelope organization. the differences were made evident through a comparative study of the outer envelope of r. tsutsugamushi, r. prowazekii, and r. rickettsii by electron microscopy. | 1978 | 101465 |
| sarcocystinae: nomina dubia and available names. | examination of the original descriptions of the species of sarcocystis in cattle, sheep, and swine, and of isosporid oocysts shed sporulated by dogs, cats, man, and other carnivores, has shown that it is not possible in most instances to identify unambiguously recently recognized taxa. the original descriptions are insufficient, and because no type specimens exist, could apply to two or more of the presently recognized taxa. we consider the following nomina dubia: sarcocystis hirsuta s. miescher ... | 1979 | 107666 |
| an apparent outbreak of rickettsial illness in costa rica, 1974. | a focal outbreak of highly fatal disease occurred in eastern costa rica in 1974. no rickettsial spotted fevers have previously been reported in costa rica. nevertheless, the available evidence points to an unidentified rickettsial pathogen, probably of the spotted fever group, as the agent responsible for this outbreak. | 1978 | 100162 |
| a kinetic study of ribulose bisphosphate carboxylase from the photosynthetic bacterium rhodospirillum rubrum. | the activation kinetics of purified rhodospirillum rubrum ribulose bisphosphate carboxylase were analysed. the equilibrium constant for activation by co(2) was 600 micron and that for activation by mg2+ was 90 micron, and the second-order activation constant for the reaction of co(2) with inactive enzyme (k+1) was 0.25 x 10(-3)min-1 . micron-1. the latter value was considerably lower than the k+1 for higher-plant enzyme (7 x 10(-3)-10 x 10(-3)min-1 . micron-1). 6-phosphogluconate had little effe ... | 1978 | 100101 |
| trifluridine in resistant human herpetic keratitis. | patients with active dendritic herpetic keratitis that was unresponsive to topical idoxuridine or vidarabine therapy were treated topically with a 1% solution of trifluridine. the dendritic ulcers healed in an average time of 6.1 days. all patients tolerated the drug well except for one patient in whom a reversible crystalline epithelial keratitis developed. | 1978 | 100084 |
| plaque formation by strains of spotted fever rickettsiae in monolayer cultures of various cell types. | some strains of spotted fever rickettsiae could be distinguished by their ability or inability to form plaques in monolayer cultures of various mammalian and avian cell types. | 1978 | 99454 |
| diagnosis of rocky mountain spotted fever by immunofluorescent identification of rickettsia rickettsii in skin biopsy tissue. | | 1979 | 113518 |
| the effect of complement depletion on bacterial lipopolysaccharide (lps)-induced hemodynamic and hematologic changes in the rhesus monkey. | lipopolysaccharide (lps) isolated from salmonella minnesota r595 or from escherichia coli 0111:b4 induces hypotension in rhesus monkeys with normal complement levels. this hypotension is accompanied by decreased total peripheral resistance. the depletion of c3 and terminal complement components by prior intraperitoneal administration of the anticomplementary protein cobra factor did not alter the hemodynamic changes which occur following the rapid injection of 5 mg/kg of r595 lps, the infusion o ... | 1978 | 99042 |
| antibacterial functions of macrophages in experimental protein-calorie malnutrition. i. description of the model, morphologic observations, and macrophage surface igg receptors. | an experimental protein-calorie malnutrition was produced in weanling sprague-dawley rats. the model resembles human malnutrition with respect to weight loss, inanition, angular stomatitis, anemia, lymphopenia, hypoproteinemia with hypoalbuminemia, and marked thymic involution. in addition, systemic invasion by gram-negative rods was documented. however, no edema was produced, and animals did not survive for longer than six weeks on the protein-deficient diet. one percent glycogen was found to b ... | 1978 | 98598 |
| alterations of body fluid compartments and distribution of tissue water and electrolytes in rhesus monkeys with rocky mountain spotted fever. | chair-restrained rhesus monkeys (macaca mulatta) were inoculated subcutaneously with 10(2)--10(3) plaque-forming units of virulent rickettsia rickettsii. the latent period for fever and rickettsemia was three to four days; death occurred six to eight days after infection. total circulatory electrolyte levels and fluid volumes, including plasma, red blood cell, true circulatory blood, and extracellular fluid, increased. the expansion of the extracellular and plasma volumes resembled findings repo ... | 1978 | 98595 |
| antigen-induced neutrophil chemotactic activity in man. correlation with bronchospasm and inhibition by disodium cromoglycate. | we have previously reported increased neutrophil chemotactic activity in sera obtained after positive antigen inhalation responses in atopic subjects. this report describes the kinetics of appearance of this serum activity and the effects of antigen dose and disodium cromoglycate pretreatment on the response in 10 ragweed-sensitive subjects. significantly increased chemotactic activity was present as early as 1 min, peaked at 10 min, and persisted through 24 hr after inhalation of antigen. the i ... | 1978 | 98551 |
| evaluation of a killed rocky mountain spotted fever vaccine in cynomolgus monkeys. | a nonhuman primate model of rocky mountain spotted fever infection was developed in cynomolgus monkeys (macaca fascicularis) infected by the subcutaneous route or by aerosol. clinical responses, hematology and serum chemistry values, and pathological findings were similar to those found in humans ill with rocky mountain spotted fever. the clinical model was then used to test the efficacy of a killed rocky mountain spotted fever vaccine grown in chicken embryo cells. monkeys were immunized with v ... | 1979 | 120877 |
| a study on the relationship dose-effect in case of gamma-irradiation on leptospira. | | 1977 | 98416 |
| [1st case of rocky mountain fever in costa rica. central america]. | | 1979 | 121621 |
| release of dopamine in the nucleus accumbens of rats by thyrotropin releasing hormone (trh) [proceedings]. | | 1978 | 96901 |
| [morphological and biochemical studies on the retinal blood vessels of streptozotocin diabetic rats (author's transl)]. | | 1975 | 130794 |
| laboratory diagnosis of rocky mountain spotted fever by immunofluorescent demonstration of rickettsia in cutaneous lesions. | direct immunofluorescent staining for rickettsia rickettsii was performed on cryostat sections of skin biopsies from 27 patients suspected of having rocky mountain spotted fever. in nine of the 17 patients whose final diagnosis was rocky mountain spotted fever, coccobacillary forms of r. rickettsii were identified in endothelium and vascular walls within the dermis. facotrs recognized as contributing to false-negative results were prior treatment with tetracycline or chloramphenicol for 24--48 h ... | 1978 | 96691 |
| [intravenous cholangiography in hyperbilirubinaemia (author's transl)]. | the effect of bilirubin on biliary and renal excretion of methylglucamine-iodipamide at an infusion rate of 1.3, 2.6 and 5.2 mu mol/min/kg was studied by intravenous injection into five dogs with normal liver functions and chronic duodenal fistulas. unconjugated bilirubin administered in a dose of 0.1 mu mol/min/kg reduced biliary iodipamide excretion and concentration significantly, but did not affect renal contrast material excretion. this indicates that the unsatisficatory radiological result ... | 1979 | 153309 |
| ultrastructural localization of calcium around the membrane of the surface connected system in the human platelet. | the localization of calcium in the membrane system of human platelets was determined by ultrahistochemical methods equipped with an electron probe x-ray microanalyzer. after potassium oxalate-glutaraldehyde treatment large amounts of electron opaque precipitates were observed around the membrane of the surface connected system. electron probe x-ray microanalysis clearly defined that the precipitates were composed of calcium oxalate. the localization of calcium on the membrane of the surface conn ... | 1978 | 96043 |
| effect on rats and mice of a diet with low tryptophan content (maize flour). | female wistar rats when crowded together become aggressive if fed a diet with low tryptophan (trp) content (maize, flour, mf). when isolated during 30 days and fed mf they show a decrease of weight, of total plasma trp, and of trp and 5-hydroxytryptamine (5-ht) in brain stem, but 5-hydroxyindolacetic acid (5-hiaa) of the same cerebral area does not change. these results partially agree with other authors observations. mice isolated during 47 days and fed with mf do not show any significant weigh ... | 1979 | 95611 |
| characterization of 40,000- and 25,000-dalton intermediate precursors to rauscher murine leukemia virus gag gene products. | under steady-state labeling conditions, rauscher murine leukemia virus-infected nih swiss mouse cells contain at least three major polyproteins derived from the viral gag gene. they have molecular weights of 65,000, 40,000, and 25,000. they have been termed ppr65gag, pr40gag, and ppr25gag. ppr65gag has been shown by a number of laboratories to be composed of all four core proteins (p15, pp12, p30, and p10). in this paper, pr40gag was found to contain p30 and p10 antigenic determinants and peptid ... | 1979 | 94357 |
| prolonged survival of corneal allografts in rabbits treated with cyclosporin a. | | 1979 | 90771 |
| in-phase brain activities during arousal and sleep. | eeg of sensorimotor area, emg of neck muscles and unit activity in lateral geniculate (lg) nucleus, recorded in freely moving cats, were analysed for the presence of correlations between them. the 3 activities were first integrated with a 2 sec time constant, then a correlation coefficient was assessed between time-linked points of the 3 records for periods of 40 sec. the average vlaues of the correlation coefficients were found to increase from arousal through slow synchronized sleep (s sleep), ... | 1979 | 89948 |
| [allergy and lectins: action between ige-mediated histamine release and glycoproteins from viscum album l. (mistletoe)]. | low-molecular constituents from viscum album (toxic) release, in irritatively toxic manner, histamine from human leukocytes without destroying the cells (cytotox test). this histamine release is prevented by preincubation with viscum lectins. the viscum lectin blocks the fc-portion of allergen-specific ige against anti-ige [125i]. lectin also inhibits the allergen-specific histamine release from human leukocytes occurring through ige; this can be demonstrated on leukocytes from patients with ato ... | 1978 | 88141 |
| cell types required for h-2-restricted cytotoxic responses generated by trinitrobenzene sulfonate-modified syngeneic cells or trinitrophenyl-conjugated proteins. | murine spleen cells were fractionated over nylon wool or sephadex g-10 columns, and the cell types involved in the generation of trinitrophenyl (tnp)-specific, h-2 restricted (tnp-self) cytotoxic effector cells were studied from cultures stimulated with trinitrobenzene sulfonate (tnbs)-modified syngeneic cells, tnp-conjugated soluble proteins such as bovine gamma-globulin (tnp-bgg), or bovine serum albumin (tnp-bsa). unfractionated or nylon nonadherent responding cells generated such effectors, ... | 1979 | 87418 |
| specificity of human lymphocytes primed against allogeneic cells in vitro. ii. discrimination and cross-reactivity in repeated priming. | when studying the specificity of human lymphocytes primed in vitro against hla-d determinants on allogeneic cells, it was found that specific restimulation of the primed cells 10 days after the first priming did not influence their discriminatory power compared to cells primed only once. likewise, priming to one hla-d determinant and repeated priming against another hla-d determinant did not change the discrimination for the first priming antigen. neither was there any increased relative reactiv ... | 1979 | 87030 |
| external layers of rickettsia prowazekii and rickettsia rickettsii: occurrence of a slime layer. | using a simple specific-antibody stabilization procedure on organisms gently liberated from their host cells, we have demonstrated by electron microscopy that rickettsia prowazekii and rickettsia rickettsii possess a coat of variable thickness, external to the outer leaflet of the cell wall and the structure designated by others as a "microcapsule," which corresponds most closely to the slime layer of certain other bacteria. reactions in the methenamine silver and ruthenium red staining procedur ... | 1978 | 83297 |
| plaque assay and cloning of scrub typhus rickettsiae in irradiated l-929 cells. | it was demonstrated that gamma-irradiated l-929 cells support plaque formation by three strains of rickettsia tsutsugamushi and representative species of the spotted fever and typhus group rickettsiae. sensitivity of the plaque assay for detection of viable scrub typhus rickettsiae was similar to that achieved with intraperitoneal inoculation of random-bred mice. the concentration of irradiated cells and the temperature and length of incubation were all found to affect plaque size. a technique c ... | 1977 | 69632 |
| scanning and transmission electron microscopy of rickettsia rickettsii propagated in cell culture. | scanning electron microscopy utilizing critical-point drying and transmission electron microscopy employing air-dried agar pseudoreplicas and critical-point dried carbon replicas were used to study the surface of rickettsia rickettsii propagated in cell culture. | 1977 | 18928 |
| cytostatic activity of peripheral blood monocytes against bronchogenic carcinoma cells in patients with lung cancer. | cytostatic acitivity of peripheral blood monocytes against cultured cell lines of bronchogenic carcinoma was examined in patients with lung cancer. cytostatic activity in lung cancer patients with neither augmented nor suppressed as compared with that of controls such as normal healthy persons, patients with malignancies other than lung cancer, and patients with benign respiratory diseases. there was no correlation between the cytostatic activity of monocytes and the advance of clinical stages o ... | 1979 | 229054 |
| biological properties of antibodies against rat adipocyte intrinsic membrane proteins. dependence on multivalency for insulin-like activity. | antisera from rabbits injected with rat adipocyte plasma membranes or intrinsic proteins from such membranes, obtained by a dimethylmaleic anhydride extraction step, mimicked the action of insulin on both glucose transport and lipolysis in intact adipocytes. biological activity in both types of antisera was mediated by immunoglobulin binding to one or more intrinsic proteins of the adipocyte plasma membrane since fat cells were unresponsive to all antisera absorbed with dimethylmaleic anhydride- ... | 1979 | 107169 |
| influence of the substituent radicals in antimonyl naphthalein derivatives on the potentialities of anti-schistosomal activity in mice. | | 1979 | 262474 |
| discrepancies in weil-felix and microimmunofluorescence test results for rocky mountain spotted fever. | only 4.2% of 284 single specimens and 17.6% of 51 pairs of sera reactive in weil-felix agglutination tests for rocky mountain spotted fever were confirmed by a specific rickettsia rickettsii microimmunofluorescence test. | 1979 | 107194 |
| ascending aorta-supraceliac abdominal aorta bypass: successful removal of an infected graft in the descending thoracic aorta. | an infected graft and a mycotic pseudoaneurysm were successfully resected by employing an ascending aortasupraceliac abdominal aorta bypass graft in a 19-year-old man. he had formerly undergone graft replacement surgery for traumatic aneurysm of the descending thoracic aorta, with the aid of a temporary external bypass graft. after this first operation, the patient had suffered from septicemia due to psudomonas aeruginosa, which resulted in formation of mycotic pseudoaneurysms at the distal anas ... | 1979 | 108052 |
| [reevaluation of the effect of certain parameters on the survival of neisseria gonorrhoeae]. | lysis and survival of 4 gonococcal strains were studied under different conditions in the presence of divalent cations and glycerol. the stability and the viability of the gonococcal cells were not enhanced by any of these compounds. | 1979 | 108131 |
| antibody to b. subtilis dna polymerase iii: use in enzyme purification and examination of homology among replication-specific dna polymerases. | bacillus subtilis dna polymerase iii (pol iii), an arylhydrazinopyrimidine-sensitive, replication-specific enzyme, was used to generate a non-precipitating rabbit antibody which specifically inhibited pol iii activity in vitro. the antibody was used to examine structural relationships among several dna polymerases, and it was linked covalently to agarose; the antibody:agarose was employed to develop a rapid, selective method of purification of catalytically active b. subtilis pol iii. | 1979 | 108667 |
| rocky mountain spotted fever in connecticut: human cases, spotted-fever group rickettsiae in ticks, and antibodies in mammals. | three parameters were used in 1976 and 1977 to assess the status of rocky mountain spotted fever (rmsf) in connecticut--compilation and review of clinical data on suspected human cases for the 13-year period 1965--1977, examination of tick tissues for spotted fever-group rickettsiae by the hemolymph test and direct immunofluorescence, and analyses of mammalian sera for antibodies against rickettsia rickettsii. there were six presumptive rmsf cases which probably originated in connecticut. four o ... | 1979 | 111543 |
| exposure of guinea pigs to rickettsia rickettsii by aerosol, nasal, conjunctival, gastric, and subcutaneous routes and protection afforded by an experimental vaccine. | guinea pigs were inoculated with rocky mountain spotted fever by the aerosol, conjunctival, subcutaneous, intragastric, and intranasal routes. rickettsial infection was produced by all routes except intragastric. all animals with clinical signs of disease developed agglutinating antibody, and most developed a cell-mediated immune response. disease produced by all experimental routes (except intragastric) was indistinguishable. the tissue culture-derived inactivated vaccine produced in this labor ... | 1979 | 114488 |
| natural infection of dogs on cape cod with rickettsia rickettsii. | four isolates of rickettsiae from sick dogs on cape cod, mass., were serologically identical to isolates of rickettsia rickettsii from human patients with rocky mountain spotted fever. the antigenic analysis used the indirect fluorescent-antibody test and antisera prepared in mice to each of the isolates and to reference strains of r. rickettsii and rickettsia montana. serological responses of infected dogs were specific for r. rickettsii, although antibodies to r. montana were also detected in ... | 1979 | 114534 |
| sugar hydrolases and their arrangement on the rat intestinal microvillus membrane. | the arrangement of the sugar hydrolases, sucrase-isomaltase, maltase, and lactase on the microvillus membrane of rat intestine was investigated by immunological technique. the enzymes were purified essentially free of each other to near homogeneity and antisera of high specificity were obtained against each. microvillus membranes were prepared routinely in high purity from rat intestine and contained an average 61% protein, 20% lipid, and 19% carbohydrate, with the sugar hydrolases comprising an ... | 1979 | 116006 |
| indirect hemagglutination test for detection of antibody to rickettsia rickettsii in sera from humans and common laboratory animals. | antibody production in humans and three species of laboratory animals infected with rickettsia rickettsii was determined with the indirect hemagglutination test. rabbits, guinea pigs, and mice were inoculated with r. rickettsii and bled at intervals. antibody which agglutinated both fresh and glutaraldehyde-fixed sheep erythrocytes sensitized with antigen prepared either from purified rickettsiae or from infected yolk sacs was found in rabbit sera at all intervals tested (10 to 59 days postinfec ... | 1979 | 120875 |
| in vitro studies of rickettsia-host cell interactions: ultrastructural changes induced by rickettsia rickettsii infection of chicken embryo fibroblasts. | secondary chicken embryo fibroblasts infected with the sheila smith strain of rickettsia rickettsii and grown in monolayer culture undergo rapid morphological alterations. transmission electron microscopic examination of cells at intervals after infection showed several progressive host cell lesions, including widespread dilatation of the rough endoplasmic reticulum and outer nuclear envelope and the accumulation of electron-dense material within the cisternae of intracellular membranes. dilatat ... | 1979 | 121115 |
| the manufacture of moulded supportive seating for the handicapped. | the wheelchair-bound population often have difficulty in obtaining a correct or comfortable posture in their chairs and sometimes develop pressure sores from long-duration sitting. this problem is being solved by manufacturing personalised, contoured seats which support the patient over the maximum area possible thereby reducing the pressure on the body and the incidence of pressure sores. a cast is obtained of the patient in a comfortable, medically correct posture and from this cast the seat i ... | 1975 | 126704 |
| a fair deal for the handicapped. | | 1978 | 148882 |
| mg2+ and atp effects on k+ activation of the ca2+-transport atpase of cardiac sarcoplasmic reticulum. | atp and the divalent cations mg2+ and ca2+ regulated k+ stimulation of the ca2+-transport atpase of cardiac sarcoplasmic reticulum vesicles. millimolar concentrations of total atp increased the k+-stimulated atpase activity of the ca2+ pump by two mechanisms. first, atp chelated free mg2+ and, at low ionized mg2+ concentrations, k+ was shown to be a potent activator of atp hydrolysis. in the absence of k+ ionized mg2+ activated the enzyme half-maximally at approximately 1 mm, whereas in the pres ... | 1979 | 162038 |
| sphingomyelinase of chicken erythrocyte membranes. | | 1975 | 165774 |
| size and charge heterogeneity of rat tissue ferritins. | ferritins purified from horse spleen and from rat liver, kidney, heart and hepatoma were analyzed by quantitative polyacrylamide gel electrophoresis. from the migration characteristics of these ferritins at several gel concentrations, ferguson plots were constructed and the molecular sizes and charges (apparent valences) together with their statistical variability were obtained by applying rodbard computer programs to the data. finally, ellipses were drawn describing the 95% confidence limits of ... | 1975 | 172145 |
| [calcium and magnesium concentrations of the renal cortex in the rat. comparison of 3 methods of tissular extraction of the principal cations]. | we describe three methods for extraction of main tissular cations : a) dry ashing method; b) wet ashing method with hno3; c) wet method on fresh tissue with hcio4. this study allows the determination of tissular concentrations of calcium, magnesium in renal cortex of the rat. our findings show : a loss of na, k cations probably by volatilization with dry ashing method ; inability for k determination when hcio4 method is used ; tissular concentrations are : 4,7 meq ca ; 21,6 meq mg per kg of wet ... | 1975 | 172839 |
| adaptation of skeletal muscle to endurance exercise. | skeletal muscle adapts to endurance excerise, such as long distance running, with an increase in the capacity for aerobic metabolism. this is reflected in an increased capacity of whole homogenates and of the mitochondrial fraction of muscle to oxidize pyruvate and long chain fatty acids. underlying this increase in the ability to obtain energy by respiration is an increase in the levels of a number of mitochondrial enzymes. these include the enzymes involved in fatty acid oxidation, the enzymes ... | 1975 | 173969 |
| transport of retinol from the blood to the retina: involvement of high molecular weight lipoproteins as intracellular carriers. | | 1976 | 179832 |
| malignant transformation-linked imbalance: decreased xanthine oxidase activity in hepatomas. | xanthine oxidase was decreased 2- to 10-fold in all examined rat hepatomas irrespective of the malignancy; growth rate and degrees of histological differentiation of the neoplasms. the affinity to substrate (km=6-8 mum) and the ph optimum (8.0) of the liver and hepatoma enzymes were the same. the reprogramming of gene expression, as manifested in the decreased activity of this key purine metabolizing enzyme, appears to be specific to neoplastic transformation. since glutamine prpp amidotransfera ... | 1975 | 179860 |
| phosphofructokinase (isozymes) activation by theophylline or by 3',5' cyclic amp administration. | phosphofructokinase activity of rat thyroid, kidney and muscle can be enhanced by in vivo administration of theophylline or cyclic amp. this enhancement occurs in the different tissues to a different extent depending on the tissue-specific isozymes. thyroid and muscle phosphofructokinase which is mainly a-type, is strongly stimulated after administering theophylline in the drinking water over a 8 days period. the kidney enzyme which encompasses about 50% of each a and b type is activated to a le ... | 1977 | 193771 |
| rickettsiae of the spotted fever group in slovakia. | | 1977 | 408981 |
| effects of budr on developmental functions of dictyostelium discoideum. | the development of dictyostelium discoideum cells, as measured by spore yield, is somewhat more sensitive to the presence of budr during vegetative growth than is growth itself. observations on the development of control and budr-grown cells, their protein labelling patterns and assays of 4 developmentally regulated proteins all reveal a consistent picture. budr appears to block spore formation by partially inhibiting several or many different earlier events during development. the relative sens ... | 1978 | 207443 |
| [seroepidemiological investigations on the prevalence of rickettsial antibodies in man in the federal republic of germany (author's transl)]. | 1,600 random serum samples from inhabitants of hesse were investigated for rickettsial antibodies by complement fixation test. antibodies were found in 19 samples (1,2%) against rmsf-group antigen with titers between 1:10 and 1:640. these results confirm recent reports on natural foci of such rickettsial infections in the federal republic of germany. antibodies against rickettsia of the typhus-group or coxiella burnetii could not be detected by the complement fixation reaction. none of the sera ... | 1977 | 410727 |
| [isolation of rickettsia of the spotted fever group (sf group) from dermacentor marginatus ticks in tirol, austria]. | | 1977 | 410728 |
| reaction of component recovered from cementum exposed to periodontal pocket with human serum. | | 1977 | 267110 |
| [comparative precipitation studies between the highly-virulent virus of european swine fever, swine-fever-virus split products and precipitating swine-fever antigens from the organs of swine-fever infected pigs]. | | 1977 | 414484 |
| anti-epidermal-cell-surface pemphigus antibody detaches viable epidermal cells from culture plates by activation of proteinase. | immunoglobulin from pemphigus patients binds to the surface of mouse epidermal cells in culture. cells incubated with the pemphigus antibody are easily detached from culture plates whereas cells incubated with serum from normal patients remain on the plate. pemphigus antibody-mediated cell detachment is blocked by the addition of the proteinase inhibitors soybean trypsin inhibitor and alpha2-macroglobulin to the culture media. detachable cells are viable, and activation of the complement cascade ... | 1978 | 272663 |
| melatonin secretion decreases during the proestrous stage of the rat estrous cycle. | urine was collected from rats during 12 consecutive daily dark periods and assayed for melatonin and norepinephrine; the phase of the vaginal estrous cycle associated with each urine sample was determined from daily vaginal smears. the proestrous phase of the estrous cycle was consistently associated with significant reductions in the excretions of both compounds. the level of melatonin in any urine sample tended to vary as a function of its norepinephrine content; however, the slope of the curv ... | 1978 | 272672 |
| distribution of binding sites for 125i-labeled alpha-bungarotoxin in normal and deafferented antennal lobes of manduca sexta. | 125i-labeled alpha-bungarotoxin has been used to determine the distribution of putative acetylcholine receptors in normal and chronically deafferented antennal lobes in the brain of the moth manduca sexta. toxin-binding sites are confined to synaptic regions in deafferented lobes. these findings suggest that receptors can develop in the insect central nervous system independently of normal synaptic influences. | 1979 | 284367 |
| activation of the kallikrein-kinin system in rocky mountain spotted fever. | activation of the kallikrein-kinin system, as indicated by increased plasma kallikrein and depleted plasma kininogen, prekallikrein, and kallikrein inhibitor, was observed in five patients with rocky mountain spotted fever. four of the patients had petechial rashes characteristic of vasculitis. three patients had alterations in coagulation consistent with disseminated intravascular coagulation, although no hemorrhagic syndrome was found. our data, along with the known physiologic actions of kini ... | 1978 | 307354 |
| cytotoxic activities of normal cultured human t cells. | normal human t cells grown in continued cultures in medium containing conditioned medium (cm) from pha-stimulated lymphocytes were studied for their ability to manifest three known forms of cell-mediated cytotoxicity: lectin-induced cellular cytotoxicity (licc), natural killer cell (nk) activity, and antibody-dependent cellular cytotoxicity (adcc). the cultured t cells (ctc) were very effective mediators of licc, being cytotoxic even at very low attacker-target cell ratios in the presence of dif ... | 1978 | 308961 |
| fc-receptors on human t lymphocytes. i. transition of tgamma to tmu cells. | | 1978 | 308968 |
| synthesis of plasma membrane proteins during development of dictyostelium discoideum. | | 1979 | 421897 |
| human bone marrow colony formation in diffusion chambers. normal values. | | 1978 | 340240 |
| a method for specific diagnosis of rocky mountain spotted fever on fixed, paraffin-embedded tissue by immunofluorescence. | for specific demonstration of rickettsia rickettsii in fixed, paraffin-embedded tissues, the technique of trypsin digestion of deparaffinized, rehydrated sections was investigated. it was determined that 3.5 hr of digestion was optimal for achieving discrete, bright, green immunofluorescence of rickettsiae. at autopsy kidneys from seven of 10 cases of probably rocky mountain spotted fever contained structures that were specifically stained and that had the size and shape of rickettsiae. these st ... | 1978 | 342642 |
| comparison of individual and cumulative dose-response curves [proceedings]. | | 1979 | 497601 |
| fatal viscerotropic rocky mountain spotted fever. report of a case diagnosed by immunofluorescence. | a case of fatal viscerotropic rocky mountain spotted fever with virtual absence of cutaneous lesions was diagnosed at autopsy by specific immunofluorescent demonstration of rickettsia rickettsii in spleen, kidney, epididymis and skin. the clinical presentation was that of insidious onset of fever, renal failure, hypotension, hyponatremia and obtundation over a 10 day period. the patient had respiratory insufficiency, hypocalcemia, increases in creatinine phosphokinase (cpk), serum glutamic oxalo ... | 1978 | 345805 |
| intestinal apoproteins during fat absorption. | to compare the roles of apolipoprotein (apo) a-i, b, and e (or arginine-rich apoprotein, arp) in the intracellular production of intestinal chylomicrons (and/or vldl), these apoproteins were localized in rat intestinal mucosa by the light microscope method of indirect immunofluorescence. in addition, tissue levels of apoa-i and apob were measured during fat absorption by radioimmunoassay. antisera were produced using apoa-i isolated from rat plasma high density lipoprotein, and apob and arp from ... | 1978 | 350901 |
| initial clinical evaluation of a new rocky mountain spotted fever vaccine of tissue culture origin. | currently available rocky mountain spotted fever (rmsf) vaccines are relatively ineffective in preventing infections in humans and contain considerable amounts of contaminating egg protein. a new formalin-inactivated vaccine was prepared by sucrose density gradient centrifugation of the sheila smith strain of rickettsia rickettsii grown in chick embryo cell tissue culture. the new product has greater protective immunogenicity in rheusus monkeys and guinea pigs than commercial vaccines. six volun ... | 1978 | 355580 |
| rhipicephalus sanguineus: vector of a new spotted fever group rickettsia in the united states. | a rickettsia related to but distinct from the spotted fever agent, rickettsia rickettsii, has been detected in 167 (18.9%) of 884 rhipicephalus sanguineus taken off dogs in central and northern mississippi. the organisms could readily be isolated in male meadow voles (microtus pennsylvanicus), where it produced massive infections in the tissues of tunica vaginalis. it was practically nonpathogenic for male guinea pigs, although inoculation of these animals with infected tunica vaginalis of voles ... | 1975 | 806533 |
| alteration in local and systemic immune capacity after exposure to bursts of co. | | 1979 | 387409 |
| a comparison of serologic methods for diagnosis of rocky mountain spotted fever. | sera referred to the north carolina division of health services for rickettsial serology in 1974 were tested by complement fixation (cf), microimmunofluorescence (micro-if), microagglutination (ma) and hemagglutination (ha) for antibodies against rickettsia rickettsii. there was good agreement among micro-if,ma and ha tests in detecting antibody responses to this agent, but the cf test was definitely less sensitive than the others, even in illnesses with classical clinical manifestations of rock ... | 1977 | 401558 |
| identification of rickettsia rickettsii in a guinea pig model by immunofluorescent and electron microscopic techniques. | moribund guinea pigs infected with richettsia rickettsii were examined by necropsy, histology, immunofluorescence, electron microscopy, and serology. untreated animals died at 9 and 10 days after inoculation. animals given saline subcutaneously survived from 1 to 4 days longer. prolonged survival was accompanied by more severe lesions: scrotal necrosis; infarction of ears; and swollen, hemorrhagic footpads, epididymis, and cremaster muscle. histopathologic examination demonstrated that acute, ne ... | 1977 | 402079 |
| rocky mountain spotted fever (tick-borne typhus) in south carolina: an educational program and tick/rickettsial survey in 1973 and 1974. | because the incidence of spotted fever is increasing in south carolina, campaigns were carried out in 1973 and 1974 to provide the public and medical practitioners, through pamphlets and news media, with information about spotted fever and the ticks which transmit the causative agent, rickettsia rickettsii. people were also invited to save and submit live ticks removed from vegetation, animals and humans, for examination by the hemolymph test. a total of 1,186 ticks consisting of 987 dermacentor ... | 1975 | 811127 |
| indirect hemagglutination test for human antibody to typhus and spotted fever group rickettsiae. | an indirect hemagglutination (iha) test is described that uses glutaraldehyde-stabilized erythrocytes treated with a rickettsial erythrocyte-sensitizing substance obtained from rickettsia typhi or rickettsia rickettsii. the serological reagent was stable for at least 3 months at room temperature and 6 months at 4 c. it exhibited group specificity and no group cross-reactivity. at a minimum dilution of 1:40, acute and early convalescent epidemic and murine typhus antisera showed 86% positive reac ... | 1975 | 811685 |
| rocky mountain spotted fever: occurrence in massachusetts. | | 1977 | 402694 |
| early diagnosis of rocky mountain spotted fever. use of primary monocyte culture technique. | diagnosis of rocky mountain spotted fever was made as early as the fourth day of febrile illness in rhesus monkeys (macaca mulatta), with a monocyte culture technique that is easily amenable to use in humans. rickettsia rickettsii were identified by direct immunofluorescent and gimenez staining of cultures of circulating monocytes from four experimentally infected monkeys, even in the presence of a mild or inapparent disease in three. levels of rickettsemia were estimated by the plaque-assay tec ... | 1976 | 814256 |