| enhancement of the antigenic activity and virulence of the vaccine strain e of rickettsia prow azeki by passages in cell culture. | changes in the biological properties of the vaccine strain e of rickettsia prowazeki occurred upon cultivation of a1 (human amnion) cells infected with this strain. in the course of passages of these cells the antigenic activity and virulence of the rickettsia increased. the changes were observed in 10 out of 22 cell cultures examined: in 6 cultures there was an increase in the antigenic activity and in 4 both in the antigenic activity and in virulence. the time of the occurrence of these change ... | 1975 | 1993 |
| ord river arboviruses--isolations from mosquitoes. | one hundred and thirty presumptive viruses have been isolated from 485 pools made from 23,872 mosquitoes collected in the ord river area of north-west australia. one hundred and eleven of the virus isolates isolates came from pools of culex annulirostis, the dominant mosquito species caught in the vicinity of kununurra. forty-five of the viruses pathogenic for newborn mice have been further characterized-19 as flaviviruses, 1 alphavirus, 9 koongol, 1 mapputta and 15 non-haemagglutinating viruses ... | 1976 | 14613 |
| light-activated calcium release from sonicated bovine retinal rod outer segment disks. | calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. the amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insensitive to the internal trapped calcium concentration. the results are obtained using a flow system in which the disk membrane vesicles are adsorbed on glass particle supported by a ... | 1977 | 14680 |
| iodination-deiodination. a radiochemical method for detection of structure and changes in structure in rna. | bound iodine is released from radioiodinated nucleotides in polymers exposed to sodium bisulfite. the rate of bisulfite-catalyzed deiodination of pyrimidines can be controlled both by change of temperature of ph and is also dependent on the molecular association of the nucleotide. the rate of release of iodine from iodocytidine in polycytidylate is greater than the rate of elimination from rna. experiments testing the influence of base-pairing of the iodopyrimidines in synthetic polynucleotides ... | 1977 | 14687 |
| altered interaction between binding and catalytic subunits of a cyclic amp-stimulated protein kinase from hepatoma cells. | | 1977 | 15518 |
| purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. | a plasminogen activator secreted by cultured human pancreatic carcinoma (mia paca-2) cells has been purified to apparent homogeneity by procedures including sepharose-l-arginine methyl ester affinity chromatography, sephadex g-200 gel filtration, isoelectric focusing, and sodium dodecyl sulfate gel electrophoresis. the plasminogen activator shares many properties with urokinase including: molecular weight (55 000), isoelectric point (8.7), heat stability (60 degrees c, 30 min), ph stability (1.5 ... | 1977 | 15590 |
| acute bacteremia in asplenic renal transplant patients. | four cases of overwhelming bacteremia were observed in asplenic renal transplant patients 12 to 20 months after transplantation. the bacteriologic findings and presentation of these infections are characteristic of post-splenectomy sepsis reported in nontransplant patients. it is suggested that the absence of the spleen, more than immunosuppression with azathioprine and prednisone, predisposes to these late, uncommon infections after transplantation. | 1977 | 16144 |
| dna and enzyme behaviour during the early period of experimental hepatic regeneration proceedings. | | 1976 | 16335 |
| serum gonadotropins in the rat after prenatal damage to the testes by busulfan and their reaction to cryptorchidism, castration and administration of testosterone. | adult male rats prenatally treated with 1,4-bis-(methanesulfonyloxy)-butane (busulfan), which damages the gonocytes, undergo cryptorchidization, castration or testosterone treatment and the fsh, lh and testosterone levels in serum are measured. basal values of these hormones do not differ from those of controls. after cryptorchidization fsh levels rise but the lh levels do not. following orchidectomy both hormone levels increase significantly. application of testosterone in various doses increas ... | 1977 | 16621 |
| cellular factors. immunologic tolerance: irradiation and bone marrow transplantation in induction of canine allogeneic unresponsiveness. | | 1977 | 17203 |
| the speed of passive dendritic conduction of synaptic potentials in a model montoneurone. | | 1977 | 17867 |
| in vitro testosterone synthesis by testicular tissue of old mouse: the metabolism of 3beta-hydroxy-5-ene steroids. | | 1977 | 18359 |
| interaction of folic acid with ribonuclease a. | | 1977 | 21693 |
| some biological properties of an endotoxic lipopolysaccharide from the typhus group rickettsiae. | a lipophilic thermostable lipopolysaccharide (lps) complex was isolated by phenol extraction from purified suspensions of the typhus group rickettsiae. the lps complex is antigenic and possesses some endotoxic properties such as toxicity for actinomycin d-treated mice, pyrogenicity for rabbits and guinea pigs, ability to elicit hypothermia in white rats and local schwartzman reaction and active cutaneous anaphylaxis in rabbits. | 1977 | 22240 |
| comparative digestibility of acid detergent fiber by laboratory albino and wild polynesian rats. | the effects of adding 5%, 10%, and 15% acid detergent fiber to a nonfibrous basal diet were examined in a comparative feeding study with polynesian rats (rattus exulans) and laboratory rats. digestibility coefficients for dry matter, crude protein, and gross energy declined significantly in both species as fiber content increased, but averaged significantly lower in the polynesian than in the laboratory rat. fiber digestibility was not significantly affected by fiber level but was by species, wi ... | 1978 | 23419 |
| tryptic digestion of immobilized d-amino acid oxidase. | | 1978 | 25787 |
| environment and selfsufficiency of elder man (author's transl). | the problems of environment of elderly people are concentrated in social area and in the intimate living space, which can not be maintained sufficiently because of increasing age and decreasing performance. the consequences of mobility in industrial revolution scattered families and also deprived old men of their helpers. inadequate living rooms and absence of technical comfort especially in the houses of the very aged men produce intense dependence of the own individual performance. medical dat ... | 1978 | 27118 |
| the effects of acridine orange on deoxyribonucleic acid in escherichia coli. | 1. acridine orange inhibits growth of escherichia coli k12 when incubated at ph 7.9, but not at ph 7.4.2. at a non-permissive temperature for dna polymerase i, acridine orange inhibits growth of a temperature-sensitive strain and also increases the rate of elimination of the f'-lac plasmid. 3. dna isolated from cells treated with acridine orange under conditions that inhibit growth contains material of low molecular weight, which is absent from dna isolated from cells treated under conditions in ... | 1978 | 27167 |
| continuous measurement of tissue ph in the human fetal scalp. | a method for continuous measurement of scalp tissue ph is described. the tissue ph probe was found to be robust and values for tissue ph were close to those for scalp blood ph. combining the ph and fetal heart fate (fhr) electrodes in the one mechanical assembly facilitated application to the fetal scalp in early labour but the combined assembly electrode was found to have some disadvantages and manufacture of a separate tissue ph electrode is recommended. continuous monitoring of scalp tissue p ... | 1978 | 29656 |
| acupuncture: its neurophysiological basis: an anaesthetist's foreword. | | 1978 | 29773 |
| blockade by n-methylhydroxylamine of activation of guanylate cyclase and elevations of guanosine 3',5'-monophosphate levels in nervous tissues. | hydroxylamine and n-methylhydroxylamine prevented the activation of soluble guanylate cyclase by the endogenous activator as well as by nitroso compounds such as n-methyl-n'-nitro-n-nitrosoguanidine or nitroprusside, while other derivaties of hydroxylamine were ineffective. hydroxylamine and n-methylhydroxylamine did not alter the basal guanylate cyclase activity of purified enzyme preparations. kinetics analysis indicated that n-methylhydroxylamine competes with n-methyl-n'-nitro-n-nitrosoguani ... | 1978 | 31192 |
| [guidelines for laevolac therapy against the endogenous factor in periodontal diseases]. | | 1978 | 33356 |
| kinetic studies in isolated organs: tools to design analgesic peptides and to analyze their receptor effects. | the opioid activities of peptide and non-peptide narcotics were studied in longitudinal muscle strip of guinea pig ileum (gpi) and in mouse vas deferens (mvd). the comparison of agonist activities of peptides found in gpi and mvd offered the opportunity to predict the presence but not the magnitude of potential analgesic activity. the kinetics of the antagonism between naltrexone and different types of agonists were also determined in these systems. using c-6 epimers of naltrexone, it was found ... | 1979 | 33396 |
| effect of growth conditions on the antigenic components of streptococcus mutans and lactobacilli. | 1) the release of lta from chemostat-grown cultures of s. mutans and l. fermentum is dependent on the generation time and ph. 2) the growth rate affects the immunogenicity of lta and the wall polysaccharide of l. fermentum. 3) the growth of l. plantarum under glucose limitation influences the glucose content of the wall teichoic acid. the composition of the wall polysaccharide of s. mutans ingbritt may also be changed under these conditions. 4) the conditions of autoclaving used to release cell ... | 1978 | 33543 |
| the stimulatory effect of 5ht and the role of the paraventricular nucleus on pms induced ovulation in the immature rat. | immature rats can be induced to ovulate with pregnant mare serum (pms) as long as they weigh over 60 g. in the rats weighing less than 60 g 5-hydroxytryptamine (5ht)-60 micrograms/rat intraventricularly or 2 micrograms/rat in the paraventricular nucleus (pvn)-stimulated ovulation. injections into several other hypothalamic nuclei were ineffective. in rats weighing greater than 60 g, lesions of the pvn or injection of 10 micrograms/rat p-chlorophenylalanine into the pvn inhibited ovulation. the h ... | 1979 | 37456 |
| quantitative study on the reproduction of virulent and vaccine rickettsia prow azeki strains in cells of different origin. | vaccine and virulent strains of rickettsia prowazeki differ by the degree of reproduction in mccoy, b, and chick embryo cells but replicate to similar levels in fl cells. as distinct from the virulent breinl strain, the vaccine e strain rickettsiae permanently lost their capacity for long-term reproduction in mccoy cell cultures but retained their capacity to adsorb on to and penetrate into these cells. consequently, the reproduction of rickettsiae is limited at later stages of intracellular inf ... | 1979 | 42302 |
| the soluble antigens of rickettsia prowazeki, r. typhi and r. canada. investigation of their interrelationship by various serological methods. | the purpose of this research is the isolation of an eventual species-specific fraction from the "soluble antigen" of rickettsiae. the "soluble antigen" of r. prowazeki (breinl strain), r. typhi (wilmington strain) and r. canada were purified at 25% saturation with ammonium sulphate (psa). corresponding antisera were produced in rabbits. the serological methods used were the complement fixation, the micro-agglutination, the precipitation method in capillary tubes and the immuno-diffusion method c ... | 1975 | 52235 |
| fetal syphilis in the first trimester. | evidenc of first-trimester fetal syphilis was sought in the products of conception in a therapeutic abortion clinic. during two collection periods of one week, five patients with serologic and clinical data consistent with recent syphilitic infection were identified. their conceptuses were carefully examined by silver and immunofluorescent stains for the presence of treponema pallidum. two of these five conceptuses (9 and 10 weeks' gestation) were found to contain t. pallidum by these methods. t ... | 1976 | 56895 |
| effects of poly(1-vinyluracil) and poly(9-vinyladenine) on viral rna-directed dna polymerase. | the effects of poly(1-vinyluracil) [poly(vu)] and poly(9-vinyladenine) [poly(va)] on the rna-dependent dna polymerase activity of murine leukemia virus (moloney strain) were studied. vinyl polymers themselves cannot act as templates for the polymerase. however, if a vinyl polymer is added to a polymerase reaction mixture in which a complementary polynucleotide serves as the template, the reaction is inhibited: thus with polyribocytidylic acid as template and oligodeoxyguanylic acid as primer, ne ... | 1976 | 56995 |
| a serial study of pregnancy proteins in primigravidae. | the plasma concentrations from four 'pregnancy proteins' and three steroid hormones have been measured throughout pregnancy in 15 primigravidae. two of the proteins, human placental lactogen (hpl) and pregnancy-specific beta1-glycoprotein (psbetag), are specific for pregnancy and correlate well with the stage of gestation. it is suggested that measurement of psbetag may be useful in assessing feto-placental wellbeing. neither of the pregnancy-associated proteins, steriod-binding beta-globulin (s ... | 1976 | 57799 |
| effects of maternal immunization on the survival and postnatal development of the offspring. | immunization of females prior to mating altered the size of their litters and the incidence of postnatal death and runting, and the effect varied with the antigen used. litter size was decreased after immunization with an aggregated synthetic polypeptide or with dnp-bgg, but not with aggregated insulin. postnatal mortality was increased after immunization with an aggregated polypeptide, aggregated insulin or dnp-bgg. postnatal runting was increased after immunization with the aggregated polypept ... | 1976 | 60843 |
| electron microscopy of surface structures of rickettsia prowazeki stained with ruthenium red. | in studying surface structures of rickettsia prowazeki (e and breinl strains) by ruthenium red staining, a microcapsular layer 125-165 a thick, composed of subunits 85--100 a in diameter with a periodicity of 100--120 a as well as the inner layer of the cell wall 40--60 a thick were clearly revealed. in tangential sections of cells, subunits of the microcapsular layer were found in parallel striation arrays. these structures presumably contain acid mucopolysaccharides detectable by ruthenium red ... | 1976 | 63238 |
| characterization of the madrid e strain of rickettsia prowazekii purified by renografin density gradient centrifugation. | the avirulent madrid e strain of rickettsia prowazekii cultivated in chicken yolk sacs could be purified successfully with a renografin density gradient method developed previously for rickettsia typhi. recovery during purification, viability, and lack of contamination with host cell components were similar for the two species, although yields of r. prowazekii per yolk sac were lower. purified typhus rickettsiae provided satisfactory antigens in the complement fixation, ouchterlony double-diffus ... | 1977 | 64456 |
| [effect of several long-acting broad spectrum antibiotics on prowazek rickettsia]. | | 1976 | 64918 |
| [experimental infection of domestic animals with r. prowazeki and r. canada]. | the authors infected lambs with r. prowazeki and r. canada to ascertain their possible role in the natural infection of the animals. the lambs were infected subcutaneously with increasing doses; rickettsiemia was recorded with the aid of tests on guinea pigs and ixodidae and argasidae ticks fed on the lambs. dynamics of antibody formation was ascertained in the infected animals in the agglutination reaction and in the complement fixation test. the antigenic affinity of r. canada and rickettsia o ... | 1976 | 65076 |
| plaque assay and cloning of scrub typhus rickettsiae in irradiated l-929 cells. | it was demonstrated that gamma-irradiated l-929 cells support plaque formation by three strains of rickettsia tsutsugamushi and representative species of the spotted fever and typhus group rickettsiae. sensitivity of the plaque assay for detection of viable scrub typhus rickettsiae was similar to that achieved with intraperitoneal inoculation of random-bred mice. the concentration of irradiated cells and the temperature and length of incubation were all found to affect plaque size. a technique c ... | 1977 | 69632 |
| external layers of rickettsia prowazekii and rickettsia rickettsii: occurrence of a slime layer. | using a simple specific-antibody stabilization procedure on organisms gently liberated from their host cells, we have demonstrated by electron microscopy that rickettsia prowazekii and rickettsia rickettsii possess a coat of variable thickness, external to the outer leaflet of the cell wall and the structure designated by others as a "microcapsule," which corresponds most closely to the slime layer of certain other bacteria. reactions in the methenamine silver and ruthenium red staining procedur ... | 1978 | 83297 |
| application of histochemical and histological stains to epoxy sections: pre-treatment with potassium permanganate and oxalic acid. | | 1978 | 84320 |
| food allergy. | | 1979 | 84902 |
| cell types required for h-2-restricted cytotoxic responses generated by trinitrobenzene sulfonate-modified syngeneic cells or trinitrophenyl-conjugated proteins. | murine spleen cells were fractionated over nylon wool or sephadex g-10 columns, and the cell types involved in the generation of trinitrophenyl (tnp)-specific, h-2 restricted (tnp-self) cytotoxic effector cells were studied from cultures stimulated with trinitrobenzene sulfonate (tnbs)-modified syngeneic cells, tnp-conjugated soluble proteins such as bovine gamma-globulin (tnp-bgg), or bovine serum albumin (tnp-bsa). unfractionated or nylon nonadherent responding cells generated such effectors, ... | 1979 | 87418 |
| anaphylatoxin-induced shock and two patterns of anaphylactic shock: hemodynamics and mediators. | in the dog, different cardiorespiratory reactions were identified in two types of anaphylactic shock and in c5a-at (anaphylatoxin)-induced shock. all three types had in common a portal blood pooling with consequent decrease in the venous return, cardiac output, and arterial pressure. in anaphylaxis (a) of the first type, at a low titer of hemagglutinating antibodies, the latent period was 68 s and heart and lung function was unchanged. in the second type, at high titer, the latency was 19 s and ... | 1979 | 88165 |
| guinea pig transfer factor: restriction of macrophage migration inhibitory activity to extraordinary dilution confirmed with demonstration of diminished potency of stored preparations. | | 1979 | 88292 |
| simple, differential staining technique for enumerating rickettsiae in yolk sac, tissue culture extracts, or purified suspensions. | a differential staining method employing acridine orange-stained rickettsiae and safranin-stained standardized suspensions of shigella dysenteriae is described for enumerating rickettsiae harvested during various stages of the growth cycle. | 1979 | 88460 |
| in-phase brain activities during arousal and sleep. | eeg of sensorimotor area, emg of neck muscles and unit activity in lateral geniculate (lg) nucleus, recorded in freely moving cats, were analysed for the presence of correlations between them. the 3 activities were first integrated with a 2 sec time constant, then a correlation coefficient was assessed between time-linked points of the 3 records for periods of 40 sec. the average vlaues of the correlation coefficients were found to increase from arousal through slow synchronized sleep (s sleep), ... | 1979 | 89948 |
| [experimental cerebral aneurysms--with reference to the experimental condition and to the etiology (author's transl)]. | | 1979 | 91988 |
| reverse transcriptase-associated rnase h. part iv. pyrophosphate does not inhibit rnase h activity of amv dna polymerase. | | 1979 | 93479 |
| [why monospecific tuberculin skin test antigens have not been isolated]. | | 1979 | 93497 |
| recent advances in applied malaria immunology. | our present knowledge of cellular and humoral factors which are involved in immunity to plasmodial infections are discussed. immunization against plasmodial infection has been achieved in birds, rodents, simians, and humans. avian hosts have been immunized against gametocytes which resulted in inhibition of gametocytes within the mosquito vector. immunization of humans against plasmodial gametocytes would indirectly protect them against malaria by blocking mosquito transmission to other suscepti ... | 1979 | 93828 |
| [deoxyribonucleic acids from pasteurella multocida and pasteurella haemolytica]. | pasteurella multocida and p. haemolytica strains contain between 1.5 and three per cent phosphorus, between nine and 14 per cent nitrogen, between two and four per cent dna, and between five and 18 per cent rna, the precise figures depending on culturing conditions. high-molecular dna may be isolated by means of bacteriolysis, using deoxycholate or dodecylsulphate and the usual steps of purification, with yield and purity differing by strains. dna with sufficient purity can be obtained from seph ... | 1979 | 93955 |
| [electrophoretic mobility test in the early diagnosis of malignant tumors; findings in the prestages of cervical and breast cancers]. | a double-blind pilot study of 130 female patients was carried out to determine the feasibility and significance of the em test in the early diagnosis of carcinoma of the female genital organs and breast. early stages of cervical carcinoma (carcinoma in situ) as well as fibroid adenoma, mastopathy and breast tumors were tested and compared with the results of their manifest forms. positive results were recorded in 87.5% of the cases of middle-grade to severe epithelial dysplasia (papanicolaou iii ... | 1979 | 94155 |
| use of antisera against bovine (ncdv) and simian (sa11) rotaviruses in elisa to detect different types of human rotavirus. | two elisa systems for the detection of human rotaviruses were developed. in the first system antibodies to nebraska calf diarrhea virus (ncdv) were used for coating the solid matrix and for the preparation of the enzyme conjugate. in the second system antibodies to human rotavirus and antibodies to simian rotavirus (sa11) were used for coating the solid matrix and for the preparation of the enzyme conjugate respectively. the second elisa system proved to have a broader spectrum for the detection ... | 1979 | 94274 |
| characterization of 40,000- and 25,000-dalton intermediate precursors to rauscher murine leukemia virus gag gene products. | under steady-state labeling conditions, rauscher murine leukemia virus-infected nih swiss mouse cells contain at least three major polyproteins derived from the viral gag gene. they have molecular weights of 65,000, 40,000, and 25,000. they have been termed ppr65gag, pr40gag, and ppr25gag. ppr65gag has been shown by a number of laboratories to be composed of all four core proteins (p15, pp12, p30, and p10). in this paper, pr40gag was found to contain p30 and p10 antigenic determinants and peptid ... | 1979 | 94357 |
| effects of etafenone and antiarrhythmic drugs on na and ca channels of guinea pig atrial muscle. | since ca-mediated electrophysiological phenomena seem to be important in the generation of various arrhythmias, we quantitatively compared the effects of quinidine, disopyramide, procainamide, lidocaine, propranolol, and etafenone, a new coronary vasodilator with antiarrhythmic properties, on na and ca channels in normal and depolarized guinea pig atria. the maximum rate of rise of the normal action potential and the amplitude of the depolarized, ca-mediated action potential were measured to exa ... | 1979 | 94412 |
| potentiation and inhibition of ganglionic transmission by ruthenium red. | the effect of ruthenium red, 2.5 to 5 mum, on ganglionic transmission in rat superior cervical ganglia and frog abdominal ganglia were studied in vitro. in rat ganglia, ruthenium red caused a spontaneous firing of ganglia cells, and an increase in the amplitude and duration of the compound action potential following a single stimulus volley. however, transmission following a conditioning volley or a repetitive stimulus train to the preganglionic nerve was depressed up to 60 sec. the asynchronous ... | 1979 | 94531 |
| enzymatic activity and qualitative immunochemical analysis of human dental plaque. | | 1979 | 94565 |
| studies on the role of vasopressin in blood pressure control of spontaneously hypertensive rats with established hypertension (shr, stroke-prone strain). | we investigated the role of arginine-vasopressin (avp) in maintaining the blood pressure of spontaneously hypertensive (sh) rats (stroke-prone strain) with established hypertension (22--28 weeks of age). in comparison with normotensive wistar kyoto (wky) rats, plasma avp concentrations of sh rats with benign hypertension (bh) were elevated twofold and in rats with severe or malignant hypertension (s-mh), fourfold. the height of the blood pressure was quantitatively related to plasma avp in both ... | 1979 | 94626 |
| neutral protease activity in lymphocytes of lewis rats with acute experimental allergic encephalomyelitis. | lymphocytes from popliteal and inguinal lymph nodes of lewis rats with acute eae as a result of injection of lyophilized guinea pig myelin in freund's complete adjuvant exerted strong proteolytic activity at neutral ph toward myelin basic protein. after injection of myelin the level of proteolytic activity remained about the same as that in lymphocytes from freund's adjuvant-injected controls until about day 10 after injection, just before the onset of paralytic symptoms; then the proteolytic ac ... | 1979 | 94652 |
| false negative hla-dr assignment necrokidney donors. a glucocorticoid effect? | thirteen potential necrokidney donors were hla-dr typed using peripheral blood b lymphocytes (pbbl). antigen assignment was impossible in 10 cases because of non-reactivity of the pbbl with the hla-dr testserum set, in some of the cases in spite of positive reactions with a positive control xenogeneic anti-human b cell antiserum. in five of these 10 donors, hla-dr typing was also performed on b lymphocytes isolated from the spleens, and in all five cases strong specific reactions were obtained, ... | 1979 | 94708 |
| transferable drug resistance of escherichia coli isolated from infant diarrhoea. | the resistance to several drugs was determined in 29 pathogenic strains of escherichia coli (026, 055 and 0111) isolated from infant diarrhoea and 18 non-pathogenic e. coli strains isolated from the same individuals. both pathogenic and nonpathogenic strains were resistant to at least 1 to 10 drugs, but only in four cases resistance patterns of the pathogenic strains were identical with those of non pathogenic ones. the majority of the strains were resistant to sulfonamide, tetracycline, ampicil ... | 1979 | 94745 |
| the effects of metiamide and h1 receptor blocking agents on anaphylactic response in guinea-pigs. | the effects of metiamide and of four h1 receptor blocking agents (mepyramine, promethazine, clemastine and ketotifene) on anaphylactic reaction were studied in the guinea-pig. the h1 blockers conferred partial protection which shows that with the experimental protocol utilized (challenge injection with high doses of antigen), histamine plays a lesser role than other mediators released or synthesized. metiamide (30.0 mg/kg i.v.) noticeably enhanced the increase in pulmonary resistance observed du ... | 1979 | 94763 |
| obtention of tritium-labelled human spectrin ; study of the secondary structure by circular dichroism [proceedings]. | | 1979 | 94805 |
| [effects of different concentrations of chloramphenicol on rna synthesis in e. coli]. | in the presence of 2 microgram/ml chloramphenicol the rate of total rna synthesis increases 1,6-fold, while that of rrna synthesis--2,5--3,0-fold. other ribosomal antibiotics, e.g. oxytetracycline, neomycin and puromycin, as well as chloramphenicol stimulate the synthesis of rna while not causing a complete inhibition of the protein synthesis. intensive stimulation of the rate of rrna synthesis with low concentrations of chloramphenicol may be due to a decrease of intracellular concentration of ... | 1979 | 94838 |
| [characteristics of human dental pulp cells in cell cultures]. | the generation time and the division rate were measured with routine methods of cell culturing. the conditions contributing to the stagnation of growth were examined; the synchrony of the growth process was investigated. the biochemical composition of the cells was assessed via colorimetry and then compared with other cell systems. the cell culture examined may be used to test capping materials; only cells up through the eighth generation however should be utilized. | 1979 | 94854 |
| a simplified short-term technique for detection of chemical carcinogens: microsomal degranulation by tannic acid. | | 1979 | 94904 |
| the effect of cyclophosphamide on a secondary ocular immune response in rabbits. | treatment of rabbits with immunosuppressive doses of cyclophosphamide during the primary response to intravitreally injected bovine gamma globulin did not prevent priming for a secondary response to the same antigen. high antibody titers were found in the aqueous humor and serum of most of the treated rabbits. ocular tissue plaque forming cell numbers were similar to those of the controls in many of the rabbits, and slightly lower in others. treatment with cyclophosphamide completely suppressed ... | 1979 | 94972 |
| biochemical effects of the modification of nucleic acids by certain polycyclic aromatic carcinogens. | | 1979 | 95052 |
| [on the serology and immunbiology of listeriosis. vii. communication: further investigations on the agglutination-immobilization test (author's transl)]. | factor antisera oriented to different serovars of listeria moncytogenes, expecially to serovar 7 and murray grayi (listeria grayi) were tested. it was found that the agglutination-immobilization test detects antibodies oriented to the h-antigen. by contrast, the growth inhibition test measures antibodies oriented to the o-antigen. summarizingly, it can be stated that the following three seroreactions, each based on a different mode of action, are available for the determination of antibodies spe ... | 1979 | 95075 |
| tetracaine blocks the responses of isolated acinar cells from rat parotid to carbachol but not to substance p. | carbachol and substance p stimulated 45ca2+ flux changes, 86rb+ efflux, and amylase secretion from acinar cells isolated from rat parotid. the local anesthetic tetracaine blocked all of these measured responses to carbachol, but none of the responses to substance p. tetracaine must act at either the cholinergic receptor or at a subsequent transducing step in the cholinergic stimulus-response sequence. if tetracaine acts at one of the transducing steps between cholinergic receptor occupation and ... | 1979 | 95097 |
| tumor immunology in domestic animals. | | 1979 | 95155 |
| an efficient procedure for serial nucleic acid hybridizations by titration analysis. | a simplified and efficient procedure has been developed for performing hybridization reactions and analyses of the percentage of hybridization on glass plates. this method is as accurate as conventional methods, while considerably reducing the time and costs to perform the hybridization in titration hybridization experiments. | 1979 | 95241 |
| an investigation of whether septal gamma-aminobutyrate-containing interneurons are involved in the reduction in the turnover rate of acetylcholine elicited by substance p and beta-endorphin in the hippocampus. | | 1979 | 95417 |
| effect on rats and mice of a diet with low tryptophan content (maize flour). | female wistar rats when crowded together become aggressive if fed a diet with low tryptophan (trp) content (maize, flour, mf). when isolated during 30 days and fed mf they show a decrease of weight, of total plasma trp, and of trp and 5-hydroxytryptamine (5-ht) in brain stem, but 5-hydroxyindolacetic acid (5-hiaa) of the same cerebral area does not change. these results partially agree with other authors observations. mice isolated during 47 days and fed with mf do not show any significant weigh ... | 1979 | 95611 |
| verification of human fetal breathing with phased array ultrasound imaging. | continuous observation of fetal movement patterns is a novel and useful capability of new high-speed ultrasound imaging systems. confirmatory documentation of human fetal breathing as a component of these movement patterns has been achieved using a phased-array real time ultrasound sector scanning system. this system permits direct visualization of fetal breathing movements. permanent recordings can be made on videotape. recordings can also be made as a time-motion echocardiograms during continu ... | 1978 | 96144 |
| 5-thio-d-glucose is an acceptor for udp-galactose : d-glucose 1-galactosyltransferase. | | 1978 | 96819 |
| [intracellular distribution of two lysosomal enzymes in newborn rat liver]. | the distribution of two lysosomal markers (beta-acetylglucosaminidase and acid phosphatase) between liver fractions was studied in the newborn rat. the results indicate that after birth the hydrolase-bearing particles increased in size and had a lower density than the primary lysosomes. these modifications may be related to the autophagic-vacuole formation known to occur during this period. | 1978 | 96999 |
| [the effects of testosterone and estradiol on the activity of lysosomal enzymes in rat liver and kidneys]. | in in vitro tests with lysosomes isolated from the liver and kidneys of castrated rats of both sexes the action of testosterone and beta-estradiol in concentrations of 3.76.10(-4)m on the activity of beta-glucosidase, beta-galactosidase and acid phosphatase was investigated. testosterone is shown to reduce the total and free activity of the membrane-bound enzymes and to increase the release from the matrix lysosomes. estradiol proved less active than is testosteron. the renal lysosomes in vitro ... | 1978 | 97095 |
| impaired host resistance to endotoxin and malaria in polychlorinated biphenyl- and hexachlorobenzene-treated mice. | the in vivo effect of polychlorinated biphenyl (pcb) and hexachlorobenzene (hcb) on murine endotoxin sensitivity and resistance to malaria (plasmodium berghei nyu-2) infection was studied. the dietary administration of 167 ppm (167 microgram/g) of pcb 1242 or hcb for 3 weeks resulted in an enhanced sensitivity to gram-negative endotoxin (salmonella typhosa), which was further increased in animals maintained on the diets for 6 weeks. by 6 weeks, a 5.2- or 32-fold increase in endotoxin sensitivity ... | 1978 | 97225 |
| [aspects of the biochemistry of the urine in a newborn infant with "typical" valinuria and the effect of ingestion of valine on amino acid transport at the level of the brain in animals]. | | 1978 | 97690 |
| a modification of the lowry procedure to simplify protein determination in membrane and lipoprotein samples. | | 1978 | 98070 |
| effect of surgical stress on serum thyroid hormones in hyperthyroidism. | the effect of elective non-thyroidal surgery on serum total and free triiodothyronine (t3), reverse t3 and thyroxine (t4) has been studied in one patient with mild, untreated typical hyperthyroidism, one with mild t3 toxicosis and in four euthyroid patients. the high t3 level of typical hyperthyroidism became normal post-operatively, decreasing to 47% of the mean pre-operative level 24 hours after surgery, while total reverse t3 doubled. in euthyroid subjects t3 decreased to 57 +/- 6 (sem)% of t ... | 1978 | 98141 |
| a study on the relationship dose-effect in case of gamma-irradiation on leptospira. | | 1977 | 98416 |
| immunochemical analysis of the conformational properties of intermediates trapped in the folding and unfolding of bovine pancreatic trypsin inhibitor. | | 1978 | 98640 |
| thyrotropin and prolactin secretory patterns during 24-hours infusion of thyrotropin-releasing hormone in calves. | plasma levels of thyrotropin (tsh), prolactin (prl), growth hormone (gh), thyroxine (t4), and triiodothyronine (t3) were measured in response to continuous 24-h infusion of synthetic thyrotropin-releasing hormone (trh) in normal and surgically thyroidectomized (thyx) calves in a series of 2 experiments. in the 1st experiment, the low dose of trh (0.077 microgram/min) had no effect on any hormone levels measured. plasma tsh concentration increased significantly (p less than 0.05) in response to t ... | 1978 | 98724 |
| location of the genes for human heavy chain immunoglobulin to chromosome 6. | immunoglobulin synthesis was examined in 31 man-mouse hybrid clones produced by fusing rag mouse cells with human lymphoid cells. cells were grown in serum-free medium containing [(14)c]leucine and a (14)c-labeled amino acid mixture. spent medium was dialyzed, concentrated, and subjected to radioimmunoelectrophoresis. eighteen clones were found to produce material that gave a radiolabeled precipitin line with anti-human igg (gamma-chain specific). production of material which was indistinguishab ... | 1978 | 98768 |
| [induction of estrus and ovulation in prepuberal young pigs by treatment with gonadotropin]. | | 1978 | 98941 |
| microradiographic demonstration of pyelolymphatic backflow in the porcine kidney. | pyelolymphatic backflow occurs when intrapelvic pressure rises acutely. although peripelvic and hilar lymphatics have occasionally been demonstrated during intravenous urography and retrograde pyelography, the patterns of intrarenal microlymphatic filling have not previously been studied radiographically in multipapillary kidneys. microradiography was performed after retrograde ureteral injections of barium sulfate suspension in recently excised pig kidneys. contrast filled the ducts of bellini ... | 1978 | 98998 |
| antigenic relationships between the typhus and spotted fever groups of rickettsiae. | paired sera from cases of epidemic typhus in ethiopia and from probable cases of rocky mountain spotted fever (rmsf) in the united states were examined by microagglutination (ma) and microimmunofluorescence (micro-if) tests for antibodies against rickettsia prowazekii, rickettsia typhi, rickettsia canada, rickettsia rickettsii, rickettsia conorii and ricksettsia akari. igg and igm antibodies against the various rickettsiae were titrated with specific fluorescein-conjugated anti-igg and anti-igm ... | 1978 | 99029 |
| brucellosis in elk. ii. clinical effects and means of transmission as determined through artificial infections. | | 1978 | 99525 |
| [indirect hemagglutination test at the ultrastructural level]. | a study on ultra-thin sections was made of the preparations of agglutinate produced during the reaction of the immunoglobulin erythrocytic diagnostic agent with dry corpuscular rickettsia prowazeki antigen, fluoresceine isothiocyanate labeled, and also srbc used for the preparation of the diagnostic agent after formalinization, tannin treatment, sensitization with hyperimmune horse serum immunoglobulins and lyophilization, respectively. formalin and tannin treatment of erythrocytes failed to be ... | 1978 | 99928 |
| pharmacological study on tetramethyl pyrazine. | | 1978 | 100295 |
| comparative ultrastructural study on the cell envelopes of rickettsia prowazekii, rickettsia rickettsii, and rickettsia tsutsugamushi. | rickettsia tsutsugamushi differs from other rickettsiae in its cell envelope organization. the differences were made evident through a comparative study of the outer envelope of r. tsutsugamushi, r. prowazekii, and r. rickettsii by electron microscopy. | 1978 | 101465 |
| surface proteins of typhus and spotted fever group rickettsiae. | six proteins, previously established as major constituents of intact organisms, were identified in cell envelopes obtained from intrinsically radiolabeled rickettsia prowazekii. extrinsic radioiodination of intact organisms conducted at 0.5 micronm iodide indicated that protein 4 was the most peripheral, although protein 1 also had reactive groups exposed on the surface of the organisms. a 10-fold increase in iodide concentration resulted in labeling of protein 2, and at 50 micronm iodide, all s ... | 1978 | 101466 |
| phospholipid composition of rickettsia prowazeki grown in chicken embryo yolk sacs. | the phospholipid composition and phospholipid fatty acid composition of purified rickettsia prowazeki were determined. the lipid phosphorous content was 6.8 +/- 1.3 microgram/mg of total rickettsial protein. the major phospholipid was phosphatidylethanolamine (60 to 70%); phosphatidylglycerol constituted 20%, and phosphatidylcholine constituted 15%. small amounts of phosphatidylserine and cardiolipin were detected. the principal fatty acids were 18:1, 16:1, and 16:0. the fatty acid composition o ... | 1978 | 101511 |
| absence of hydrogen peroxide production by or catalase action in rickettsia prowazeki. | glutamic acid oxidation by rickettsia prowazeki is not accompanied by hydrogen peroxide generation, nor is added hydrogen peroxide degraded, as measured by a manometric technique. | 1978 | 101521 |
| quantitative structure-activity relationships in the 2,4,5-ring substituted phenylisopropylamines. | the potency of a series of 2,4,5-ring substituted phenylisopropylamines was examined using the rabbit hyperthermia assay. an excellent correlation (r = 0.99) was found between the rabbit hyperthermic and human psychotomimetic potencies. in the hyperthermic model, the 4-x-substituted-2,5-dimethoxyphenylisopropylamines were found to be one to two orders of magnitude more potent than the 2-x- or 5-x-substituted positional isomers (x = -h, -ch3, -sch3, -br). conformational perturbations induced by s ... | 1978 | 101881 |
| [importance of bacteriological examinations for mycobacterium tuberculosis of certain groups of population with increased risk of contracting tuberculosis]. | | 1978 | 101984 |
| high-affinity binding of beta-alanine to cerebral synaptosomes might involve glycine-receptors. | high-affinity, na+-independent binding of beta-alanine to a synaptosomal fraction of rat brain was potently inhibited by glycine and by some other alpha-amino acids, but not by taurine or gaba. this binding mechanism, which was also sensitive to both bicuculline and strychnine, might involve synaptic receptors for both beta-alanine and glycine. | 1978 | 102523 |
| topographic localisation of insulinogenic and insulinoprival areas in the hypothalamus. | electrical stimulation of hypothalamic areas through stereotaxically implanted electrodes were carried out in conscious male rhesus monkeys. there was a significant increase in immunoreactive insulin (iri) following laterla hypothalamus (lha) stimulation. an opposite response was obtained from ventromedial hypothalamus (vmh). thus, insulinogenic and insulinoprival responses were obtained from feeding and satiety, suggesting a significant role of insulin in the regulation of food intake. | 1978 | 102526 |
| [effect of antibiotics and combined preparations on the toxic properties of rickettsia prowazekii]. | | 1978 | 102906 |
| [effect of prodigiozan on the course of experimental rickettsial intoxication]. | | 1978 | 102909 |