the influence of temperature and ph on the growth of rickettsia conorii in irradiated mammalian cells.the temperature range for optimum growth of rickettsia conorii in suspension culture of gamma-irradiated l cells was 32-38 degrees c, resulting in rickettsial doubling times between 4.1 and 6.0 hr. an asynchronous release of r. conorii from host cells was suggested by the constant increase in percent cells infected over a 36 hr period. rickettsial growth was optimal at neutral to slightly alkaline extracellular ph levels. a moderately acidic ph, however, resulted in an increase in doubling time ...197935961
application of histochemical and histological stains to epoxy sections: pre-treatment with potassium permanganate and oxalic acid. 197884320
specificity of human lymphocytes primed against allogeneic cells in vitro. ii. discrimination and cross-reactivity in repeated priming.when studying the specificity of human lymphocytes primed in vitro against hla-d determinants on allogeneic cells, it was found that specific restimulation of the primed cells 10 days after the first priming did not influence their discriminatory power compared to cells primed only once. likewise, priming to one hla-d determinant and repeated priming against another hla-d determinant did not change the discrimination for the first priming antigen. neither was there any increased relative reactiv ...197987030
cell types required for h-2-restricted cytotoxic responses generated by trinitrobenzene sulfonate-modified syngeneic cells or trinitrophenyl-conjugated proteins.murine spleen cells were fractionated over nylon wool or sephadex g-10 columns, and the cell types involved in the generation of trinitrophenyl (tnp)-specific, h-2 restricted (tnp-self) cytotoxic effector cells were studied from cultures stimulated with trinitrobenzene sulfonate (tnbs)-modified syngeneic cells, tnp-conjugated soluble proteins such as bovine gamma-globulin (tnp-bgg), or bovine serum albumin (tnp-bsa). unfractionated or nylon nonadherent responding cells generated such effectors, ...197987418
[allergy and lectins: action between ige-mediated histamine release and glycoproteins from viscum album l. (mistletoe)].low-molecular constituents from viscum album (toxic) release, in irritatively toxic manner, histamine from human leukocytes without destroying the cells (cytotox test). this histamine release is prevented by preincubation with viscum lectins. the viscum lectin blocks the fc-portion of allergen-specific ige against anti-ige [125i]. lectin also inhibits the allergen-specific histamine release from human leukocytes occurring through ige; this can be demonstrated on leukocytes from patients with ato ...197888141
systemic hyalinosis (juvenile hyaline fibromatosis). ultrastructure of the hyaline with particular reference to the cross-banded structure.systematic hyalinosis (juvenile hyaline fibromatosis) is characterized by hyalinized skin lesions. electron microscopic examination of a hyalinized skin tumor from a 19-year-old man with this syndrome revealed that the hyaline was composed of ruthenium red-positive ultrastructures (granules, filaments, and a kind of cross-banded structure), indicating the presence of glycosamino-glycan or glycoprotein, and a small number of thin collagen fibrils. using a new ruthenium red staining method combine ...197988923
inhibition of spontaneous transformation of rat embryo cells releasing endogenous type c virus by virus-specific antiserum.noninbred sprague-dawley rat embryo cell clones predictably undergo transformation after 20-30 in vitro passages following spontaneous release of endogenous rat leukemia virus (ralv). in the presence of ralv-specific antiserum, virus production and infectivity were reduced and transformation was delayed from 6 to 25 weeks. transformation was not associated with an increased expression of kirsten murine sarcoma virus-related src gene rna.197989211
prolonged survival of corneal allografts in rabbits treated with cyclosporin a. 197990771
effect on rats and mice of a diet with low tryptophan content (maize flour).female wistar rats when crowded together become aggressive if fed a diet with low tryptophan (trp) content (maize, flour, mf). when isolated during 30 days and fed mf they show a decrease of weight, of total plasma trp, and of trp and 5-hydroxytryptamine (5-ht) in brain stem, but 5-hydroxyindolacetic acid (5-hiaa) of the same cerebral area does not change. these results partially agree with other authors observations. mice isolated during 47 days and fed with mf do not show any significant weigh ...197995611
[3h]serotonin release: an improved method to measure mast cell degranulation.a method based on the release of tritium-labelled serotonin by activated mast cells in rodents is described. mast cells incorporate labelled serotonin selectively and release the label after activation by non-specific stimulators (compound 48/80, polymyxin b sulphate, atp, bovine chymotrypsin and l-alpha-lysophosphatidylcholine) or anaphylactic antibody and the corresponding antigen. these two types of activation were investigated in comparison with the toluidine blue microscopic rat mast cell d ...197896185
release of dopamine in the nucleus accumbens of rats by thyrotropin releasing hormone (trh) [proceedings]. 197896901
a study on the relationship dose-effect in case of gamma-irradiation on leptospira. 197798416
antigenic relationships between the typhus and spotted fever groups of rickettsiae.paired sera from cases of epidemic typhus in ethiopia and from probable cases of rocky mountain spotted fever (rmsf) in the united states were examined by microagglutination (ma) and microimmunofluorescence (micro-if) tests for antibodies against rickettsia prowazekii, rickettsia typhi, rickettsia canada, rickettsia rickettsii, rickettsia conorii and ricksettsia akari. igg and igm antibodies against the various rickettsiae were titrated with specific fluorescein-conjugated anti-igg and anti-igm ...197899029
growth characteristics of atypical mycobacteria in water and their comparative resistance to disinfectants.with the increasing significance of group iv atypical mycobacteria as etiological agents in a variety of infections, studies were conducted to determine their growth capabilities in water and their comparative resistance to disinfectants used to decontaminate hospital equipment. isolates of mycobaterium chelonei (tm strains) from peritoneal fluids of patients and peritoneal dialysis machines were able to multiply in commercial distilled water, with generation times at 25 degrees c ranging from 8 ...1978104656
specific labelling of replicating spp1 dna: analysis of viral dna synthesis and identification of phage dna-genes.specific labelling of replicating bacteriophage spp1 dna can be achieved by infection at nonpermissive temperature of a b. subtilis strain carrying the initation mutation dnab ts134. under these conditions host dna synthesis is reduced by 90 to 95%. this technique was used to identify cistrons of spp1 involved in phage dna synthesis and to define intermediates in spp1 replication.1978105242
increased levels of dihydrofolate reductase in rifampin-resistant mutants of bacillus subtilis.several independent, spontaneous rifampin-resistant mutants of bacillus subtilis were isolated and found to have an increased resistance to trimethoprim, an inhibitor of dihydrofolate reductase. this increased resistance in the rif mutants was the result of a specific threefold increase in the activity of dihydrofolate reductase, since six other enzymes examined remained unchanged. this increased level of dihydrofolate reductase and the trimethoprim resistance were cotransformed (100%) with the ...1979106041
ascending aorta-supraceliac abdominal aorta bypass: successful removal of an infected graft in the descending thoracic infected graft and a mycotic pseudoaneurysm were successfully resected by employing an ascending aortasupraceliac abdominal aorta bypass graft in a 19-year-old man. he had formerly undergone graft replacement surgery for traumatic aneurysm of the descending thoracic aorta, with the aid of a temporary external bypass graft. after this first operation, the patient had suffered from septicemia due to psudomonas aeruginosa, which resulted in formation of mycotic pseudoaneurysms at the distal anas ...1979108052
antibody to b. subtilis dna polymerase iii: use in enzyme purification and examination of homology among replication-specific dna polymerases.bacillus subtilis dna polymerase iii (pol iii), an arylhydrazinopyrimidine-sensitive, replication-specific enzyme, was used to generate a non-precipitating rabbit antibody which specifically inhibited pol iii activity in vitro. the antibody was used to examine structural relationships among several dna polymerases, and it was linked covalently to agarose; the antibody:agarose was employed to develop a rapid, selective method of purification of catalytically active b. subtilis pol iii.1979108667
3'-terminal nucleotide sequence of alfalfa mosaic virus rna 4.the sequence of the 3'-terminal 91 nucleotides of alfalfa mosaic virus rna 4, the messenger for the viral coat protein, has been elucidated. a fragment containing the 3' terminus of the rna was obtained by mild digestion with rnase t1. the primary structure of the fragment was deduced by labeling it in vitro at its 5' terminus and application of rna sequencing techniques. the sequence is completely extracistronic and is believed to contain the binding sites for the viral coat protein and replica ...1979108677
[clinical conference: histamine and basophils after cellular separation on albumin gradient]. 1975126441
observations on epizootiology and distribution of elaeophora schneideri in montana ruminants.seventy-four moose, 111 elk, 20 mule deer, 8 white-tailed deer, 26 prong-horn antelope, 42 domestic sheep and 3 bighorn sheep from montana or northwestern wyoming were examined post-mortem for evidence of elaeophora schneideri infection in 1973-74. fifteen percent of the mule deer and four percent of the moose were positive for adult arterial worms. this constitutes the first report of e. schneideri in mule deer in montana. no gross signs of blindness or other neurologic disorder were evident in ...1975127848
[morphological and biochemical studies on the retinal blood vessels of streptozotocin diabetic rats (author's transl)]. 1975130794
studies of the histochemistry, ultrastructure, motor innervation, and regeneration of mammalian intrafusal muscle fibres. 1976137430
recovery of preimplantation blastocysts in the squirrel monkey by a laparoscopic technique.a laparoscopic uterine flushing technique has been developed for the recovery of preimplantation embryos from the squirrel monkey. fourteen adult female squirrel monkeys were induced to ovulate with 4 or 5 days' treatment with follicle-stimulating hormone (1 mg) followed by human chorionic gonadotropin (hcg) (500 iu). natural mating with a fertile male or artificial insemination was performed near the time of ovulation. thirty-six hours and eleven days after hcg administration, the females were ...1977140077
preparation of human immunoglobulin free of plasmin and anticomplement activities.human igg separated by cohn fractionation showed variability in the content of aggregates, plasminogen and anticomplement activity. the plasminogen was removed or markedly reduced by affinity chromatography on sepharose-lysine. anticomplement activity was reduced by chromatography of cohn fraction ii on deae-cellulose. preparations of igg obtained by chromatography of intermediates from cohn fractionation (e.g. cohn fii + fiii or fii + fiii w) on deae-cellulose were devoid of aggregates, plasmin ...1977140530
a fair deal for the handicapped. 1978148882
guinea-worm infection in banaskantha district of gujarat--some important epidemiological aspects. 1978153328
the cornea in normal condition and in groenouw's macular dystrophy. 1979161528
mg2+ and atp effects on k+ activation of the ca2+-transport atpase of cardiac sarcoplasmic reticulum.atp and the divalent cations mg2+ and ca2+ regulated k+ stimulation of the ca2+-transport atpase of cardiac sarcoplasmic reticulum vesicles. millimolar concentrations of total atp increased the k+-stimulated atpase activity of the ca2+ pump by two mechanisms. first, atp chelated free mg2+ and, at low ionized mg2+ concentrations, k+ was shown to be a potent activator of atp hydrolysis. in the absence of k+ ionized mg2+ activated the enzyme half-maximally at approximately 1 mm, whereas in the pres ...1979162038
letter: petechiae: due to adenovirus type 7 infection or to acetylsalicylic acid therapy? 1975165451
modification of surface membrane antigens by trypsin.cultured 3t3 and py-3t3 mouse fibroblasts were subjected to mild treatment with twice crystallized trypsin and their immunogenicity tested in icr/cd-1 mice. surface antigenic modifications were observed using the foot pad swelling assay for detection of delayed hypersensitive responses. mice immunized with trypsin-treated 3t3 cells showed cell-mediated immune reactions following challenge with either homologous antigen or untreated py-3t3 fibroblasts. in contrast, treatment of the virus-transfor ...1975165549
isolation of a glycogen synthase i kinase that is independent of adenosine 3':5'-monophosphate.three protein kinases (atp:protein phosphotransferase, ec were detected when the soluble fraction of rabbit kidney medulla was chromatographed on deae-cellulose with a linear nac1 gradient. the first two kinases eluted (peak 1 and peak ii) were cyclic-amp-dependent, wheras peak iii was cyclic-amp-independent. a procedure was developed to separate the catalytic subunit of peak ii cyclic-amp-dependent protein kinase (representing the bulk of the histone kinase activity) from peak iii pro ...1975166380
[seeking for the mechanism of thermophily and thermostability (author's transl)]. 1975167400
surface and bulk characteristics of a polyether urethane for artificial hearts.a segmented polyether urethane was used as the blood contacting surface in a series of 10 heart assist devices implanted in calves for periods up to 35 weeks. at termination, each was examined to correlate blood compatibility and device performance with surface properties, chemical purity, physical stability and affinity for lipid absorption.1975170287
[study of the process of acth and insulin interaction with the plasmatic membranes of rat liver cells in vitro using luminescence methods].the process of interaction of acth and insulin with plasmatic cellular membranes of the rat's liver and artificial lipid membranes (liposomes) was studied by the method of proper proteinic fluorescence and by using hydrophobic fluorescent probes. this process is shown to be attended by conformative readjustments of the membranous proteins and also by definite structural changes in the lipid phase of the membranes. in its action acth involves the hydrophobic layer of the lipid phase to a greater ...1976179856
[virologic longitudinal study in chronic respiratory tract diseases. ii. isolation of viruses]. 1976180407
inflammatory cells in solid murine neoplasms. i. tumor disaggregation and identification of constituent inflammatory cells.mechanical and enzymatic methods of disaggregating tumors were studied with the goals of (1) minimizing cell losses while (2) maintaining functional and surface membrane markers needed to objectively identify inflammatory cells (ic)1 in resultant suspensions. application of the principles and methods described makes accurate estimation of the percentage of each ic type present in neoplasms possible for the first time. compared to purely mechanical means of disaggregating tumors, all enzyme mixtu ...1976182647
studies of peptides. lx. synthesis of the nonatriacontapeptide corresponding to the entire amino acid sequence of bovine adrenocorticotropic hormone. 1976186201
regulation of norepinephrine release from cardiac sympathetic fibers in the dog by presynaptic alpha- and beta-receptors.the effect of phenoxybenzamine (pba), desmethylimipramine (dmi), clonidine (clnd), sotalol (stl), and isoproterenol (ispr) on the release of endogenous norepinephrine (ne) from the heart on right cardioaccelerator nerve stimulation was studied in anesthetized dogs. under control conditions, the catecholamine levels in coronary sinus blood increased linearly with increasing frequencies of stimulation up to 10 hz and did not increase further at 30 hz. the release of ne was markedly enhanced after ...1977193655
a linkage study of rabbit serum high density lipoprotein (hdl) allotypes.close linkage between the two allotypic systems of rabbit serum high density lipoprotein (hdl), hl 1 and r 67, has been confidently excluded, the recombination fraction being at least 0-20. no suggestion of linkage between either hdl allotype and the immunoglobulin allotypes a1a2a3/b4b9, or the red blood cell system (rbc) adf was obtained. close linkage was confidently excluded for the hl 1-adf, hl 1-a1a2a3, r 67-adf, r 67-a1a2a3, r 67-b4b9, a1a2a3-b4b9, and b4b9-adf relationships.1977194985
the presence of disulfide-linked gp70-p15(e) complexes in akr murine leukemia virus. 1977201090
secretory granules of the duodenal enterochromaffin cells of the cat. 1979222959
cytostatic activity of peripheral blood monocytes against bronchogenic carcinoma cells in patients with lung cancer.cytostatic acitivity of peripheral blood monocytes against cultured cell lines of bronchogenic carcinoma was examined in patients with lung cancer. cytostatic activity in lung cancer patients with neither augmented nor suppressed as compared with that of controls such as normal healthy persons, patients with malignancies other than lung cancer, and patients with benign respiratory diseases. there was no correlation between the cytostatic activity of monocytes and the advance of clinical stages o ...1979229054
ph1-positive polycythemia vera.the rare presence of the ph1 chromosome in chronic myeloproliferative disorders other than chronic granulocytic leukemia (cgl), i.e. polycythemia vera (pv), myeloid metaplasia with myelofibrosis (mmm) and hemorrhagic thrombocytopenia (ht) raised the question whether or not the ph1 chromosome is peculiar to cgl. in an attempt to answer this question, the authors reports six cases of positive-ph1 of which two are from their personal experience and four from the literature. three of these six cases ...1977265077
serological diagnosis of mediterranean spotted fever.complement fixation and indirect micro-immunofluorescence (if) tests were compared for specificity and sensitivity in detection of antibodies against rickettsia conorii, in acute and convalescent human sera. purified particulate rickettsial antigens were used. good agreement between these two tests was found. use of specific anti-igg and anti-igm sera in parallel if tests made it possible to differentiate between recent and past infections.1979384863
human plasma angiotensinogen: a review of purification procedures.the current status of the purification and characterization of human angiotensinogen is reviewed. one problem encountered in the past has been the copurification of a protein with similar porperties. this protein has tentatively been designated alanine-protein. an efficient separation of angiotensinogen and alanine-protein was obtained on a zinc chelate column. alanine-protein has been purified and its amino acid and carbohydrate composition determined. the cooh-terminal amino acid and the nh2-t ...1979390363
lung compliance and lung morphology following artificial ventilation in the premature and full-term rabbit neonate.rabbit neonates delivered on day 27--30 of gestation were subjected to intermittent positive pressure ventilation (ippv) for 60 min. tidal volume was standardized to 10 ml/kg, and the insufflation pressure required to maintain this tidal volume was recorded. the quasistatic compliance of the lung-thorax system, corrected for variations in body weight, was significantly lower in animals delivered on day 27--28 than in those delivered on day 29--30. immature animals with low lung-thorax complianc ...1979394314
[the molecular biology of aging -- 15: structure and function of the genetic apparatus in aging. ii. synthesis and metabolism of rna].with aging in different organs there are similar or tissue specific changes of the ribonucleic acids in respect of the quantity, the spectrum of different rna species and the metabolism of rna. among these processes the template function of the chromatin and the behaviour of the rna polymerases are important influencing factors. in aging the sensitivity of the rna synthesis against hormonal stimulation is decreased in the most cases. a survey about the results of many in vivo and in vitro experi ...1979395770
[cellular composition of the lymph nodes of monkeys (rhesus macaque) under normal and experimental conditions].by means of mathematical methods, quantitative and qualitative changes were studied in different structural components of the mesenteric (ileocecal) lymph nodes in normal monkeys (macaca rhesus) and under per os administration of salmonella typhi murium, streptomycin-dependent. cellular composition was calculated in the cortical plateau, cortical (lymphoid) cords and in follicules. average percent of every cell type was determined. vaccine administration, was stated to inhibit cytopoiesis in the ...1978415703
nucleic acid and protein metabolism in the pancreas, spleen, thymus, lung, cardiac and skeletal muscle and lens of undernourished female rats. 1979533871
nicolas andry award. increased tissue pressure and its effects on muscle oxygenation in level and elevated human limbs.increased tissue pressure is an important cause of local circulatory compromise. in both rabbit and human model systems known external pressures were applied to otherwise normal limbs. side-by-side comparison of the wick and the infusion techniques revealed that both methods of pressure measurement yielded essentially identical results when tissue pressure was elevated. measured tissue pressure significantly exceeded the external pressure applied to the limb. using a mass spectrometer-teflon mem ...1979535242
[characteristics of human neutrophils obtained by the chamber method of "skin window"].viability, morphology and function of healthy human cells migrated into the chamber through the "skin window" were studied. after 18 to 20 hours there were 42.0 +/- 5.3 (x 10(6))/cm2 viable cells characterized by a high content of mature neutrophils (98.6 +/- 0.6%). normal reactivity of migrated neutrophils was observed using the nitroblue tetrazolium reduction test. the chamber variant of the "skin window" method is recommended as a simple physiological technique for preparing pure populations ...1979540158
[electron microscopical evidence of spiroplasma citri in nucleus of infected testplants (author's transl)].this is the first report for the evidence of mycoplasmas in an organelle of a plant cell. spiroplasma citri was detected by electron microscopy in the nucleus of a phloem parenchyma cell of chrysanthemum carinatum schousboe plants, inoculated experimentally by euscelis plebejus fall.1979549392
proteins of typhus and spotted fever group rickettsiae.purified radioactive rickettsiae were obtained from irradiated and cycloheximide-inhibited l cells, and their proteins were analyzed by polyacrylamide gel electrophoresis. rickettsial species could be distinguished by comparing the relative mobilities of constituent proteins after migration of two differentially labeled preparations in a single gel. distinct differences were observed in gel patterns of rickettsiae from the typhus and spotted fever groups, as well as with different species within ...1976820639
changes in monoamine oxidase and monoamines with human development and aging.a series of studies of monoamines and their metabolism in a variety of human tissues indicate that there are aging effects that may alter neurotransmitter substances. monoamine oxidase (mao) activity has a significant positive correlation with age in plasma and blood platelets of normal subjects and patients suffering from depressive disorders. monoamine oxidase and age correlate positively in hindbrain and in eight separate ares of human brains from patients who died from a variety of causes. h ...19751088942
proceedings: cell-wall composition of aerobic cutaneous "diphtherios" organisms. 19751142405
comparative morphology of genital cones of genus ancylostoma dubini, 1843.male worms of ancylostoma braziliense, a. ceylanicum, a. kusimaense, a. malayanum, a. duodenale, a. caninum, a. tubaeforme, agriostomum vryburgi and cyclodontostomum purvisi have the external appendages beside the anogenital aperture. these anogenital structures are morphologically similar and are assumed to be homologous among the species with three pairs of teeth or more. in hookworms with two pairs of teeth, the anogenital structures are complex. anogenital features can be used as a taxonomic ...19751166351
attenuated, streptomycin-dependent salmonella typhi oral vaccine: potential deleterious effects of lyophilization.four studies were done with streptomycin-dependent salmonella typhi as an oral, attenuated vaccine. studies 1 and 3 employed freshly harvested vaccine, whereas studies 2 and 4 involved lyophilized vaccine. five to eight doses (3 x 10(10)-10(11) organisms/dose) were given; oral streptomycin (1.0 g) was administered concomitantly in studies 2 and 3, with only two of the doses of vaccine in study 1, and was not given in study 4. no adverse reactions were encountered in 179 vaccinated men, and 94% o ...19761262709
intracellular movements of rickettsia conorii and r. typhi based on actin polymerization.human vascular endothelial, vero and human embryonic lung cells infected with rickettsiae for 24 h or 48 h were labelled for polymerized actin with nbd-phallacidin. between 20 and 68% of the intracellular rickettsia conorii had an actin tail of between 0.33 and 15 microns, with the longest tails being observed in vero cells. in the case of r. typhi less than 1% of the organisms had actin tails and these were considerably shorter than those of r. conorii. these findings provide new information co ...19921299836
expression and release of neuroregulators during development: monoamines and neuropeptides.the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ...19921306802
characteristics of neisseria gonorrhoeae isolated in australia showing decreased sensitivity to quinolone antibiotics.forty three strains of neisseria gonorrhoeae with decreased sensitivity to quinolone antibiotics were detected amongst 2141 australian isolates of gonococci examined in the years 1984 to 1990. the strains examined belonged to 23 different auxotype/serovar classes, were generally more resistant to other antibiotics and, in the majority of cases, were isolated from travellers entering or returning to australia from se asia. quinolone-sensitive wild-type gonococci became less sensitive to these age ...19921315946
leukotriene b4 omega-oxidation by human polymorphonuclear leukocytes is inhibited by pyocyanin, a phenazine derivative produced by pseudomonas aeruginosa.human polymorphonuclear leukocytes (pmnl) metabolize the potent chemotaxin leukotriene b4 (ltb4) by omega-oxidation to 20-hydroxyl-ltb4 and 20-carboxy-ltb4. the ability of unstimulated human pmnl to metabolize exogenous ltb4 was found to be inhibited by pyocyanin, a phenazine derivative produced by pseudomonas aeruginosa, in a dose-dependent manner. 1-hydroxyphenazine (1-ohp), a metabolite of pyocyanin, was not inhibitory under identical conditions. the initial enzymic step in the conversion of ...19921316878
new monoclonal antibodies for the detection of immediate early antigens of cytomegalovirus.two new monoclonal antibodies, cie-1 and cie-2, were developed for the rapid detection of human cytomegalovirus (hcmv) infection. they were found to be reactive with immediate early protein of hcmv in the nuclei of infected fibroblasts, as early as 3 hours post-infection. by radioimmunoprecipitation, cie-1 was found to react with a protein with an apparent molecular weight of 70,000, whereas cie-2 precipitated 2 proteins of 70,000 and 72,000 daltons, respectively. both monoclonal antibodies reco ...19921319172
viral role in cervical and liver cancer.hepatitis b virus (hbv) and certain human papillomaviruses (hpv) have been suspected of playing a role in human malignancy. the long latency period (decades) and the fact that only a small proportion of infected individuals subsequently have cancer suggests that these viruses probably contribute in an indirect manner to the development of cancer. analysis of viral gene function at the molecular level supports this conclusion. more studies are needed to understand the subtle interactions between ...19921325282
von willebrand factor release and thrombomodulin and tissue factor expression in rickettsia conorii-infected endothelial cells.mediterranean spotted fever, a tick-borne rickettsiosis caused by rickettsia conorii, may lead to small-vessel or deep-vein thrombosis. in order to evaluate the role of endothelial cell alteration in this lesion, we infected human endothelial cells derived from umbilical veins with r. conorii. we report the induction of two previously unreported prothrombotic mechanisms in rickettsial disease: (i) a progressive decline in thrombomodulin antigen and (ii) early expression of tissue factor, and, as ...19921328057
susceptibility of rickettsia conorii, r. rickettsii, and coxiella burnetii to pd 127,391, pd 131,628, pefloxacin, ofloxacin, and ciprofloxacin.plaque formation and dye uptake assays were used to measure the mics of pd 127,391 and pd 131,628 against rickettsia species. the mics of pd 127,391 were 0.25 microgram/ml for rickettsia rickettsii and 0.125 to 0.25 microgram/ml for rickettsia conorii. the mics of pd 131,628 were 0.25 to 0.5 microgram/ml for r. rickettsii and 0.5 microgram/ml for r. conorii. as determined by the shell vial technique, 15 strains of coxiella burnetii were susceptible to pd 127,391 and pd 131,628 (mic, < or = 1 mic ...19921336950
identification of spotted fever group rickettsiae using polymerase chain reaction and restriction-endonuclease length polymorphism order to facilitate the isolation and identification of spotted fever group (sfg) rickettsiae from their tick vectors, we used the centrifugation shell vial technique or traditional isolation procedures and genotypic identification using the restriction fragment length polymorphism analysis of polymerase chain amplified fragments. the presence of rickettsia conorii both in rhipicephalus sanguineus ticks collected in southern france, and in rhipicephalus simus and haemaphysalis leachi from zim ...19921350164
the effect of monocyte-derived macrophages on the growth of rickettsia conorii in permissive cells.we examined whether monocyte-derived macrophages (mdm) incubated with rickettsia-infected hep-2 or bgm cells a) affect r. conorii (boutonneuse fever) growth, and b) secrete tnf and il-1 alpha. bgm and hep-2 cells were infected with r. conorii at multiplicities of infection (moi) of 1-0.01. after 2 hr of adsorption, the cells were washed and mdm were added at an effector to target ratio of between 3 and 5. at 2, 24, 48, and 96 hr post-infection (p.i.) cells were scraped off; cell-free medium was ...19921350166
human and tick spotted fever group rickettsia isolates from israel: a genotypic analysis.the genomes of spotted fever group rickettsiae isolated in different geographical areas of israel (two from ticks and four from humans, obtained over a span of 20 years) were studied by polymerase chain reaction (pcr) and restriction endonuclease fragment length polymorphism (rflp) analysis. the human isolates were obtained from patients suffering from rickettsial disease of different degrees of severity. the pcr products obtained with five pairs of oligonucleotide primers (two primer sets deriv ...19921356998
biochemical and functional characterization of the leucocyte tyrosine phosphatase cd45 (cd45ro, 180 kd) from human neutrophils. in vivo upregulation of cd45ro plasma membrane expression on patients undergoing haemodialysis.the biochemical and functional characterization, and the regulation of plasma membrane expression of the leucocyte tyrosine phosphatase cd45, have been investigated in neutrophils from healthy donors and patients undergoing haemodialysis. cd45 proteins of 180 kd and 130-150 kd were precipitated from neutrophils from both healthy subjects and haemodialysed patients. prolonged storing, as well as trypsin treatment of samples containing the 180-kd cd45 protein, generated the 130-150-kd polypeptides ...19921370931
antigenic diversity of rickettsia conorii.analysis of seven strains designated as rickettsia conorii for reactivity with a panel of 12 monoclonal antibodies to surface-protein epitopes of spotted fever group rickettsiae and by western immunoblotting with standard serotyping sera revealed remarkable antigenic diversity. rickettsial strains from france, morocco, ethiopia, kenya, south africa, india, and the ussr differed from one another in reactivity with at least one and as many as five monoclonal antibodies. simko and indian strains we ...19921379008
evidence of the presence of spotted fever group rickettsiae in dogs and dog ticks of the central provinces in assess canine exposure to spotted fever group rickettsiae in the central provinces of spain, ticks removed from dogs were studied by immunofluorescence (if) staining. twenty-eight out of 65 (43.0%) samples from ticks were positive. sera from 58 dogs were also collected and the presence of antibodies to rickettsia conorii studied. thirty-four (58.6%) dogs presented significant titers by if. surveillance data from these provinces show the presence of boutonneuse fever cases during recent years, ...19921397228
experimental infection of dogs with a zimbabwean strain of rickettsia conorii.seroconversion was demonstrated in dogs artificially infected with a zimbabwean strain of rickettsia conorii. no haematological or biochemical abnormalities were found and the only clinical signs observed were pain, erythema and oedema at the inoculation site and regional lymphadenopathy. intermittent rickettsaemia was detected using the centrifugation shell vial technique up to 10 days post inoculation.19921404553
atypical papulovesicular rash due to infection with rickettsia conorii.we present an unusual case of rickettsia conorii infection that was associated with cutaneous papulovesicular lesions on a patient who had returned from the bushveld of south africa. the lesions were diffusely scattered across the trunk, extremities, and both palms. several recent reports have documented similar papulovesicular or pustulovesicular rashes that occurred on travelers returning from southern africa. these rashes resemble the lesions of rickettsialpox. evidence suggests that these at ...19921420671
pretargeted immunoscintigraphy: effect of hapten valency on murine tumor uptake.a method of radioimmunoscintigraphy using bivalent "janus" haptens with an apparent enhanced affinity ("avidity") for the antibody is described. janus with 50 micrograms pretargeted mab wc3a11 resulted in significantly higher murine tumor concentrations (approximately 7%/g) compared to monovalent haptens (approximately 1.4%/g, p < 0.001), and the same high tumor-to-background ratios (approximately 3/1). janus was synthesized by coupling two molecules of babe together with a 1,4 butanedithiol lin ...19921432163
development and persistence of cowdria ruminantium specific antibodies following experimental infection of cattle, as detected by the indirect fluorescent antibody test.different breeds of cattle were experimentally infected with palm river, a zimbabwean isolate, or ball-3, a south african isolate of cowdria ruminantium, derived from tissue culture or tick or blood stabilates. c. ruminantium specific antibody responses were detected by an indirect fluorescent antibody test (ifat) using c. ruminantium-infected bovine aortic endothelial (bae) cell cultures as antigen. the first detection of antibodies to c. ruminantium generally coincided with the peak of the feb ...19921441219
[boutonneuse fever in switzerland: apropos of a case report].the history and clinical presentation of mediterranean spotted fever in a 72-year-old male patient with polycythemia vera are described. the patient presented soon after arrival in bordeaux (france) with fever, arthralgia and an erythematous maculopapular skin rash involving the palms and foot soles. the clinical course was complicated by pulmonary involvement resulting in dyspnea and hypoxemia. the patient denied tick bite but had contact with a dog. antibiotic therapy with doxycycline resulted ...19921462146
use of western blot to analyze the reactivity of sera from patients with mediterranean spotted fever.the reactivity of antigenic components of rickettsia conorii with sequentially obtained sera from 20 adult spanish patients with mediterranean spotted fever was analyzed by western blot. the major rickettsial antigens reacting with the serum samples corresponded to molecular weights of 135 and 115 kda. these antigens constantly exhibited higher staining intensity than the other antigens, and reacted with 100% and 86.7%, respectively, of acute sera and with 100% of convalescent phase samples. ric ...19921486892
diagnosis of mediterranean spotted fever by indirect immunofluorescence of rickettsia conorii in circulating endothelial cells isolated with monoclonal antibody-coated immunomagnetic beads.rickettsia conorii, an obligate intracellular bacterium that infects vascular endothelial cells, is the etiologic agent of mediterranean spotted fever (msf). a new procedure using indirect immunofluorescence was used to directly detect r. conorii in circulating endothelial cells (cec). cec were separated from other blood components by using anti-endothelial cell monoclonal antibody-coated magnetic beads. an anti-r. conorii polyclonal rabbit antiserum was used to stain rickettsiae. the entire pro ...19921500755
comparison of western immunoblotting and microimmunofluorescence for diagnosis of mediterranean spotted serum samples from 41 patients suffering from mediterranean spotted fever (msf) were tested by microimmunofluorescence (mif) and western blot (wb; immunoblot). immunoglobulin g (igg), igm, and iga antibody-specific responses to the high-molecular-mass species-specific protein antigens (115 kda and 135 kda) of rickettsia conorii, as well as to cross-reactive lipopolysaccharide (lps) antigens, were observed. the wb assay detected igm-type antibodies earlier than did the mif assay. thes ...19921537916
[rickettsia conorii encephalitis]. 19921560696
characterization of rickettsial attachment to host cells by flow cytometry.the mechanisms of rickettsial attachment have been studied by measuring quantitative changes in rickettsial binding to host cells by flow cytometry after different treatments of the rickettsiae and host cells. time-dependent binding of rickettsia conorii to host cells was demonstrated by the increasing intensity of host cell surface fluorescence of rickettsia-host cell combinations when examined with a rickettsia-specific monoclonal antibody. more than 70% of host cells had intensity of fluoresc ...19921563794
spotless boutonneuse fever.mediterranean spotted fever is endemic in southern france, especially during summer. clinical diagnosis is generally based upon the presence of a febrile eruption with or without the typical tache noire. usual laboratory findings, which include thrombocytopenia, elevated levels of hepatic enzymes, and hyponatremia, are not specific to the disease. the diagnosis may be confirmed serologically by obtaining specific western immunoblot results and by isolation of rickettsia conorii from blood cultur ...19921571413
role of lagomorpha in the wild cycle of rickettsia conorii in salamanca (spain).in an endemic zone for mediterranean spotted fever levels of antibodies to r. conorii were evaluated in serum samples from wild rabbits (orytolagus cuniculus) and hares (lepus granatensis) using an indirect microimmunofluorescence antibody test. the results of the study show that the wild rabbit may carry out in this area an important function in the maintenance of r. conorii in nature.19921572424
astrakhan fever rickettsia is identical to israel tick typhus rickettsia, a genotype of the rickettsia conorii complex. 19921583342
[the ionized calcium in the blood of domestic chickens: dependence on age and sex].in the present study concentration of ionized calcium and the correlation between total and ionized calcium in blood of domestic fowl and its dependence on age and sex were investigated. furthermore a possible influence of blood sampling procedure on the measured values was assessed. leghorn chickens of either sex, aged 3, 9, 25, and 80 weeks, respectively, were used. blood sampling was performed by vein-puncture and indwelling catheters, respectively, of both ulnaris vein and artery of animals ...19921590033
isolation of an agent of the spotted fever group rickettsia from tick eggs in madrid, spain.ticks recovered from dogs in rural villages around madrid (spain) were processed to isolate rickettsiae. one sample containing mixtures of ticks and four containing eggs, in which rickettsiae had been detected by indirect immunofluorescence with a human serum highly reactive to rickettsia conorii, were decontaminated, homogenized and inoculated onto vero cells. two egg samples yielded a cytopathic agent that reacted positively by immunofluorescence. one sample (14h) was successfully subcultured ...19921601085
rickettsial keratitis in a case of mediterranean spotted fever.the first documented case of infectious keratitis (an ameboid-like corneal ulcer) caused by rickettsia conorii is described. corneal infection was probably caused by contamination through the tears during systemic rickettsial dissemination. topical tetracyclin ointment was effective. rickettsial keratitis should be considered in the differential diagnosis of ameboid-like corneal ulcers in areas where mediterranean spotted fever is endemic.19921638167
meningoencephalitis caused by rickettsia report of mediterranean spotted fever in an 18 year old male heroin addict, caused by rickettsia conorii. the typical "tache noir" was absent. the patient developed meningoencephalitis within 5 days after onset of the systemic symptoms. diagnosis was based on serological findings. treatment with doxycycline led to rapid recovery without sequelae.19911665772
rickettsia conorii isolated from ticks introduced to northern france by a dog. 19911672015
histopathology and immunopathology of skin biopsy specimens in mediterranean spotted fever.histology of skin lesions and demonstration in them of rickettsia conorii by direct immunofluorescence test (dif) are presented in 13 patients with mediterranean spotted fever (msf). the lymphohistiocytic vasculitis which dominated the picture is not specific, however, it could be suggestive for the diagnosis of rickettsiosis. by dif we demonstrated rickettsial coccobacillary forms in all the patients: in 12 macular lesions and in one "tache noire". the diagnosis was also confirmed by indirect i ...19911687640
comparison of serologic methods for the diagnosis of mediterranean spotted fever.during summer mediterranean spotted fever (msf) is prevalent in the subcostal part of croatia (north dalmatia) as well as in other areas of the mediterranean coast. we compared the specificity and sensitivity of complement fixation (cf), latex agglutination (latex-r. conorii), microimmunofluorescence (micro-if) and enzyme-linked immuno assay (elisa) for detection and measuring antibodies against rickettsia conorii in 78 sera from 46 patients with/or suspected mediterranean spotted fever. the ser ...19911687643
species-specific monoclonal antibodies to rickettsia japonica, a newly identified spotted fever group rickettsia.a total of 192 hybridomas were developed from mice immunized with rickettsia japonica, a newly identified spotted fever group rickettsia pathogenic for humans. of these hybridomas, 101 were species specific, 37 were spotted fever group reactive, and the other 54 were also reactive with one or more of the other pathogenic species of spotted fever group rickettsiae, rickettsia akari, rickettsia australis, rickettsia conorii, rickettsia rickettsii, and rickettsia sibrica. seven of the species-speci ...19901696280
isolation by a sensitive centrifugation cell culture system of 52 strains of spotted fever group rickettsiae from ticks collected in france.boutonneuse fever caused by rickettsia conorii is transmitted mainly by the brown dog tick, rhipicephalus sanguineus. we collected 540 ticks in marseille, france, and tried to isolate as many strains of rickettsia as possible. ticks were evaluated for the presence of rickettsia by the hemolymph test and by a new culture system, the centrifugation-shell vial technique. we avoided contamination in the culture system. prior to ticks being submitted to the hemolymph test, they were disinfected. only ...19901696286
polypeptides constituting the antigenic basis for identification of rickettsia sibirica species by the standard serotyping method for spotted fever group rickettsiae.the antigens of rickettsia sibirica, rickettsia rickettsii, and rickettsia conorii were examined by western immunoblots and the standard immunofluorescent serotyping assay with mouse anti-rickettsia sibirica serum that is used for determining the species identification of spotted fever group rickettsiae. serum was employed prior to absorption and after absorption with purified native or heated rickettsia sibirica. the unabsorbed antiserum, the antiserum absorbed with heated rickettsia sibirica a ...19901697727
intrafamilial outbreak of mediterranean spotted fever.the existence of intrafamilial outbreaks of spotted-fever group rickettsioses has seldom been reported, and their true incidence is not known. we here report the occurrence of mediterranean spotted fever (msf) in three members of a family: a boy, his grandmother and his grandfather. the infection resolved uneventfully in the boy, and followed a more protracted course in the two older patients, who suffered complications including drowsiness, clotting defects and acute renal failure. this finding ...19921732403
analysis of high psa n-cam expression during mammalian spinal cord and peripheral nervous system development.using a monoclonal antibody that recognizes specifically a high polysialylated form of n-cam (high psa n-cam), the temporal and spatial expression of this molecule was studied in developing spinal cord and neural crest derivatives of mouse truncal region. temporal expression was analyzed on immunoblots of spinal cord and dorsal root ganglia (drgs) extracts microdissected at different developmental stages. analysis of the ratio of high psa n-cam to total n-cam indicated that sialylation and desia ...19911769342
development of an in vitro microtest to assess drug susceptibility of babesia bovis and babesia bigemina.continuous cultivation of the bovine hemoparasites babesia bovis and babesia bigemina was developed as an in vitro microtest to assess parasite susceptibility to babesicidal compounds. reproducibility of parasite multiplication rates was independent of culture size, making it possible to use a microscale of 100 microliters for each test sample. inhibitory concentrations (ic50s) of a commonly used babesicide, quinuronium sulfate, evaluated by this in vitro method were found to be 5 x 10(-8) g/ml ...19911779305
[malignant mediterranean boutonneuse fever. septic shock of unknown origin?].mediterranean botonous fever (mbf) is an infectious disease which provokes multisystemic vasculitis due to endothelial proliferation of rickettsia conorii. its incidence, in our environment, has greatly increased during the last years being endemic in many large cities and their surrounding suburbs. in some cases its evolution is specially malignant resulting in septic shock, adult respiratory distress syndrome, and multiorgan failure. the purpose of this communication is to present a new case o ...19911784746
structural and biochemical changes in the sclera of experimentally myopic eyes. 19911794573
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