| search for correlates of resistance to virulent challenge in mice immunized with coxiella burnetii. | mice immunized with live phase i or phase ii coxiella burnetii, with killed phase i or phase ii organisms or with trichloroacetic acid (tcae) or phenol (pe) extracts were resistant to intraperitoneal infection with phase i c. burnetii irrespective of whether or not they displayed phase i antibody response at the time of virulent challenge. increased phagocytosis of purified phase i organisms by blood leukocytes or peritoneal exudate cells (pec) was noticed only in mice with phase i agglutinating ... | 1977 | 22238 |
| seroepidemiological investigations in domestic ruminants from egypt, somalia and jordan for the demonstration of complement fixing antibodies against rickettsia and chlamydia (author's transl). | 1450 random serum samples of domestic ruminants from egypt, somalia and jordan were investigated for complement fixing antibodies against rickettsia and chlamydia. between 1.5 and 3.4% of the samples from the animals investigated had antibodies against the rmsf-group of rickettsia, with exception of the sera from somalian cattle and sheep from jordan. antibodies against rickettsia of the typhus-group were found in 4 cattle and 1 goat from jordan and 2 sheep from egypt; by agglutination test with ... | 1978 | 28651 |
| influence of mild acid hydrolysis on the antigenic properties of phase i coxiella burnetii. | mild acid hydrolysis of phase i coxiella burnetii cells gradually cleaved off polysaccharide chains, which contain determinants of antigen 1, thus unmasking determinants of the diagnostically important antigen 2. this artificial phase i to phase ii conversion was reflected by changed serological activity in the complement-fixation (cf) test and by changed nonspecific phagocytosis. the product of mild acid hydrolysis can serve as a complex diagnostic preparation, because it reacts in the cf test ... | 1978 | 29469 |
| immunological properties of the lipopolysaccharide-protein complex of coxiella burnetii. | purified lipopolysaccharide-protein complex (lps-pc) extracted by trichloroacetic acid from phase i coxiella burnetii organisms induced in mice and rabbits fair levels of antibodies directed to antigen 1 and antigen 2, as detected by complement-fixation (cf), microagglutination (ma), opsonization-phagocytosis (op) and serum protection (sp) tests. in guinea pigs only very low levels of ma antibodies against antigen 2 were demonstrated. in rabbit serum, ma antibodies directed to antigen 2 were fou ... | 1978 | 29470 |
| glutathione synthesis and degradation in fetal and adult rat liver and novikoff hepatoma. | | 1978 | 28177 |
| analysis of antibody response and immunoglobulins in sera of rabbits and guinea pigs immunized with coxiella burnetii. | rabbit and guinea pig sera and their immunoglobulin fractions (igm and igg) were examined by complement-fixation (cf), microagglutination (ma), opsonization-phagocytosis (op) and serum protection (sp) tests at intervals after immunization with live phase i and phase ii coxiella burnetii suspensions. in general, ma antibodies, but also decreased, earlier than cf antibodies. the anamnestic immune response was higher with lower primary doses. both phase ii and phase i cf and ma antibodies as well a ... | 1977 | 18924 |
| chronic cryptic q-fever infection of the heart. | evidence of chronic coxiella burneti infection of the heart, a disease previously considered peculiar to patients with valvular heart-disease, was found in a patient during routine serological tests before resection of a ventricular aneurysm and also by isolation of the rickettsia from the resected tissue. the patient had no symptoms or signs of q-fever endocarditis and none of the laboratory evidence usually associated with it. | 1979 | 88607 |
| systemic hyalinosis (juvenile hyaline fibromatosis). ultrastructure of the hyaline with particular reference to the cross-banded structure. | systematic hyalinosis (juvenile hyaline fibromatosis) is characterized by hyalinized skin lesions. electron microscopic examination of a hyalinized skin tumor from a 19-year-old man with this syndrome revealed that the hyaline was composed of ruthenium red-positive ultrastructures (granules, filaments, and a kind of cross-banded structure), indicating the presence of glycosamino-glycan or glycoprotein, and a small number of thin collagen fibrils. using a new ruthenium red staining method combine ... | 1979 | 88923 |
| vaccination of naturally infected ewes against q-fever. | | 1977 | 15446 |
| characterization of an endotoxic lipopolysaccharide from coxiella burnetii. | phase i coxiella burnetii antigen isolated by phenol extraction from purified suspensions of c. burnetii in phase i is a complex lipopolysaccharide (lps) molecule containing substances typical of the bacterial lps. some endotoxic properties of this c. burnetii lps, namely pyrogenicity and skin epinephrine reaction in rabbits, hypothermia in white rats, lethal effect on chicken embryos or on actinomycin-d-treated mice are similar to those of lps isolated from other gram-negative bacteria. | 1976 | 5871 |
| precipitation of phase i antigen of coxiella burnetii by sodium sulfite. | | 1975 | 2000 |
| selection of chlortetracycline-resistant strain of coxiella burnetii. | | 1975 | 1997 |
| immunization against q-fever of naturally infected dairy cows. | dairy cows infected naturally with coxiella burnetii as evidenced either by presence of phase ii agglutinating antibodies in the blood or by shedding c. burnetii in the milk, were vaccinated subcutaneously with formalin-killed phase i c. burnetii organisms. attempts to demonstrate c. burnetii in the milk of vaccinated dairy cows 47 days after vaccination were negative, while continuous shedding of c. burnetii in the milk of control non-vaccinated dairy cows was repeatedly demonstrated in the cou ... | 1975 | 1994 |
| anaphylatoxin-induced shock and two patterns of anaphylactic shock: hemodynamics and mediators. | in the dog, different cardiorespiratory reactions were identified in two types of anaphylactic shock and in c5a-at (anaphylatoxin)-induced shock. all three types had in common a portal blood pooling with consequent decrease in the venous return, cardiac output, and arterial pressure. in anaphylaxis (a) of the first type, at a low titer of hemagglutinating antibodies, the latent period was 68 s and heart and lung function was unchanged. in the second type, at high titer, the latency was 19 s and ... | 1979 | 88165 |
| [allergy and lectins: action between ige-mediated histamine release and glycoproteins from viscum album l. (mistletoe)]. | low-molecular constituents from viscum album (toxic) release, in irritatively toxic manner, histamine from human leukocytes without destroying the cells (cytotox test). this histamine release is prevented by preincubation with viscum lectins. the viscum lectin blocks the fc-portion of allergen-specific ige against anti-ige [125i]. lectin also inhibits the allergen-specific histamine release from human leukocytes occurring through ige; this can be demonstrated on leukocytes from patients with ato ... | 1978 | 88141 |
| comparison of different antigenic preparations of coxiella burnetii used for antibody detection in guinea pigs. | the dynamics of antibody response in guinea pigs infected with coxiella burnetii was investigated by microagglutination (ma) and complement-fixation (cf) tests with different preparations of c. burnetii antigens. at the onset of antibody response the highest antibody titres were detected by the ma test with natural antigen 2, later on by the ma test with artificial antigen 2. throughtout the 1-year period of observation, the cf antibody levels were usually lower and, with the exception of the hi ... | 1978 | 87123 |
| studies of human igm anti-igg cryoglobulins: ii. isoelectric focusing characteristics of the reactive antigen and residual serum igg pools. | the co-isoelectric focusing characteristics of the antigen-igg and residual igg pools in the serum of patients with mixed igm:igg cryoglobulinemia have been determined. the antigen igg purified from five igm cryoglobulins and the residual igg purified from serum depleted of cryoglobulin were radiolabelled and co-isoelectric focused in polyacrylamide gels in order to determine if the igm anti-globulin reacted in order to determine if the igm anti-globulin reacted with iggs having unique physicoch ... | 1979 | 86512 |
| preganglionic neurons in the sacral spinal cord of the rat: an hrp study. | | 1979 | 86176 |
| expression in escherichia coli of chemically synthesized genes for human insulin. | synthetic genes for human insulin a and b chains were cloned separately in plasmid pbr322. the cloned synthetic genes were then fused to an escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. the insulin peptides were cleaved from beta-galactosidase, detected by radioimmunoassay, and purified. complete purification of the a chain and partial purification of the b chain were achieved. these products were mixed, reduced, and r ... | 1979 | 85300 |
| [formamidase--microheterogeneity, catalytic properties and inhibitors (author's transl)]. | formamidase from rat liver proved to be microheterogenous. after preparative isoelectric focusing in density gradient columns, two peaks of formamidase with identical substrate specificity were identified. by analytical focusing in thin layers of polyacrylamide or sephadex g-75 sf, even five bands could be separated. their isoelectric points were 4.75, 4.78, 4.82, 4.92 (main band) and 5.11, but their michaelis constants did not differ significantly (54 to 62 mumol/l). an identical molecular weig ... | 1979 | 84781 |
| a passive hemagglutination test for diagnosis of trench fever due to rochalimaea quintana. | a passive hemagglutination test devised for diagnosis of trench fever was easily performed and highly sensitive and specific. tanned sheep erythrocytes were sensitized with soluble antigen from rochalimaea quintana. the test detected antibody in six of seven cases of primary infection and in four cases of late, relapsed trench fever. titers of antibody ranged from 1:20 to 1:640. although both igm and igg antibody to r. quintana were detected by passive hemagglutination, igg appeared to be the ma ... | 1976 | 63526 |
| preparing and staining of coxiella burnetii natural phase ii antigen for the microagglutination reaction. | an improved method of preparing coxiella burnetii natural phase ii antigen of high purity enabled its staining for use in the microagglutination reaction (mar). the antigen was as specific and sensitive for detection of phase ii antibodies as the artificial phase ii c. burnetii antigen prepared by periodate treatment from purified phase i cells of c. burnetii. | 1976 | 61717 |
| editorial: chronic q fever or q fever endocarditis? | | 1976 | 58210 |
| a serial study of pregnancy proteins in primigravidae. | the plasma concentrations from four 'pregnancy proteins' and three steroid hormones have been measured throughout pregnancy in 15 primigravidae. two of the proteins, human placental lactogen (hpl) and pregnancy-specific beta1-glycoprotein (psbetag), are specific for pregnancy and correlate well with the stage of gestation. it is suggested that measurement of psbetag may be useful in assessing feto-placental wellbeing. neither of the pregnancy-associated proteins, steriod-binding beta-globulin (s ... | 1976 | 57799 |
| [immunologic comparison between some wolbachiae and research of antigentic community with other members of the order of rickettsiales]. | the antigenic relationship between some rickettsiae of arthropods, which do not transmit germs pathogenic to vertebrates was investigated by agglutination and immunofluorescence techniques. the comparison of r. melolonthae, r. tipulae, r. cetonidarum, r. grylli and of the rickettsiae of the scorpion buthus occitanus shows that the wolbachiae are divided into three serological groups. the insect crystallogen rickettsiae belong to the first group, r. grylli and the scorpion rickettsia are antigeni ... | 1975 | 53109 |
| electron microscopy studies of the limiting layers of the rickettsia coxiella burneti. | surface layers of coxiella burneti studied at a high resoulution reveal a plasma membrane and an outer surface membrane 6 to 7 nm thick, and a thin, moderately electron-dense intermediate layer associated with the inner surface of the outer membrane of many cells. this layer appears to be unaffected by lysozyme treatment. ruthenium red staining was used to delineate a layer of filamentous material external to the outer membrane; this fuzzy layer has a mean thickness of 20 nm and is not often see ... | 1975 | 47324 |
| suitability of coxiella burnetii strains for preparation of phase ii artificial diagnostic antigen for microagglutination test. | sensitivity in the microagglutination (ma) test of artificial (prepared by potassium periodate treatment) phase ii coxiella burnetii antigens depended on the c. burnetii strain used and the number of its chick embryo yolk sac passages. | 1979 | 42306 |
| inhibition by coxiella burnetii of ascites tumour formation in mice. | mice injected intraperitoneally (i.p.) with killed purified coxiella burnetii organisms were protected from ascites development and death caused by i.p. inoculation of sarcoma-180 cells. the extent of protection was a function of the relative dose of c. burnetii and tumour cells, and of the time of injection of c. burnetii. phase ii c. burnetii organisms exerted an antitumour protection at least as high as phase i c. burnetii organisms. | 1979 | 41443 |
| antibody response in man following a small intradermal inoculation with coxiella burnetii phase i vaccine. | a small inoculum (0.2 microgram) of phase i coxiella burnetii vaccine given to individuals previously sensitized to co burnetii elicited a positive skin reaction and a strong igm phase i antibody response as determined by microagglutination, complement fixation and microimmunofluorescence tests. a similar inoculum administered to nonsensitized individuals did not elitic a skin reaction nor stimulate a recognizable antibody response. serum from one of these sensitized and skin tested individuals ... | 1979 | 35962 |
| attempts at demonstration of lipopolysaccharide in phase ii coxiella burnetii. | comparison of some properties of phase i and phase ii coxiella burnetii cells suggests the presence of lipopolysaccharide (lps) also in the surface structures of phase ii cells. polysaccharide chains were released from them by mild acid hydrolysis and corpuscular residues resulting from such hydrolysis elicited in rabbits anti-lipid a-antibodies. toxicity for adrenalectomized and actinomycin d-sensitized mice was demonstrated with phase i cells, but not with much higher concentrations of phase i ... | 1978 | 35951 |
| experimental infection of hare (lepus europaeus) with coxiella burnetii and rickettsia slovaca. | hares (lepus europaeus l.) infected subcutaneously (s.c.) or per os with coxiella burnetii and s.c. with rickettsia slovaca overcame an inapparent infection accompanied by irregular rickettsaemia and distribution of rickettsiae to different organs and variable antibody response. neither riskettsia could be detected in faeces of infected animals, but c. burnetii was found in the urine of one hare, which died on day 13 post infection (p.i.). antibodies against c. burnetii persisted for one year of ... | 1978 | 30267 |
| hippocampal evoked potentials and eeg changes during classical conditioning in the rat. | hippocampal evoked potentials (eps) and eeg responses were studied in rats, using a classical conditioning paradigm (water, us), with a spatially discontiguous cs-us arrangement in order to separate the cs and goal-related responses. in early training, when the orienting score was high, the tone cs, instead of eliciting a definite ep, usually reset hippocampal theta activity in phase, i.e. theta rhythm became time-locked to cs. with further training, orienting activity (ori) decreased to the pre ... | 1979 | 88362 |
| search for epstein-barr and type c oncornaviruses in systemic lupus erythematosus. | lymphoblastoid cell lines were derived from patients with active systemic lupus erythematosus by allowing spontaneous transformation of peripheral b lymphocytes (b cells) harboring endogenous epstein-barr virus or by superinfecting peripheral lymphocytes with exogeneous epstein-barr virus. results of extensive studies aimed at identifying type c oncornaviruses in these lymphoblastoid cells were entirely negative by electron microscopy, dna-dna hybridization, reverse transcriptase assays, and coc ... | 1979 | 88943 |
| the value of alpha-fetoprotein and beta-human chorionic gonadotrophin in the management of testicular tumours. | serum alpha-fetoprotein and beta-human chorionic gonadotrophin levels were estimated in 115 patients. sixty-nine patients had non-seminomatous germ cell tumours and 34 patients had seminomas. twenty-six patients showed elevated serum makers. | 1978 | 88986 |
| a cerebello-pulvinar projection in the cat as visualized by the use of anterograde transport of horseradish peroxidase. | | 1979 | 88999 |
| prolonged survival of corneal allografts in rabbits treated with cyclosporin a. | | 1979 | 90771 |
| hormone-responsive genes of the mouse mammary tumor virus. | by optimal hormonal treatment the production of exogenously transmitted mmtv can be stimulated in vitro to different degrees, depending on cultivation conditions and origin of tumor cells. moreover, after appropriate hormonal treatment, endogenous mmtv-y can be rescued from primary cell cultures derived from dimethyl benzanthracene- and hormone-induced c57bl/10 mouse mammary adenocarcinoma, as determined by reverse transcriptase assay, distribution of 3h-uridine-labelled viral particles, immunof ... | 1979 | 94006 |
| [some aspects relating to the aflatoxin generation during the self-heating of cereals]. | in a stored batch of grain which was already affected by mould-formation tests were carried out with the known aflatoxin producer aspergillus flavus. a significantly lower aflatoxin production ensued if the mould growth was not connected with self-heating of the stored product. however, in conformity with increasing mould formation the germinating power was adversely affected and the significant signs (fatty acid number, reductive and none-reductive sugars) were influenced in the grain, irreleve ... | 1978 | 98933 |
| investigation of the chemiluminescence associated with phagocytic and intracellular killing activity of human polymorphonuclear leucocytes and monocytes. | the chemiluminescence associated with phagocytic and intracellular killing activity of human polymorphonuclear leucocytes and monocytes was investigated. a significant, opsonization-dependent increase in photon emission of polymorphonuclear leucocytes and monocytes was observed induced by engulfment of opsonized, heat-killed and living fungi (saccharomyces cerevisiae), aggregated gamma globulin, latex and india ink particles. mononuclear cells displayed only minimal enhancement of photon emissio ... | 1978 | 94028 |
| [determination of igm- and igg-antibodies to coxiella burnetii]. | | 1979 | 94131 |
| antigenic analysis of acute hemorrhagic conjunctivitis viruses (enterovirus type 70). | the antigenic characteristics of enterovirus type 70 (ev 70) were investigated by means of cross and kinetic neutralization tests (nt). twelve strains of ev 70 isolated in a period from 1971 to 1976 were analyzed using seven rabbit and one monkey hyper-immune sera. all the strains investigated were found to possess a common and prime variant antigens in varying proportions. accordingly, ev 70 isolates were devided intratypically into three antigenic sub groups; (1) prototype-like (four strain fr ... | 1979 | 94146 |
| in vivo regulation of glycolysis and characterization of sugar: phosphotransferase systems in streptococcus lactis. | two novel procedures have been used to regulate, in vivo, the formation of phosphoenolpyruvate (pep) from glycolysis in streptococcus lactis ml3. in the first procedure, glucose metabolism was specifically inhibited by p-chloromercuribenzoate. autoradiographic and enzymatic analyses showed that the cells contained glucose 6-phosphate, fructose 6-phosphate, fructose-1,6-diphosphate, and triose phosphates. dithiothreitol reversed the p-chloromercuribenzoate inhibition, and these intermediates were ... | 1978 | 101523 |
| thyroid hormone regulation of alpha-adrenergic receptors: studies in rat myocardium. | the effects of alterations in thyroid state on cardiac alpha-adrenergic receptors were investigated by the binding of [3h]dihydroergocryptine (dhe), a potent alpha-adrenergic antagonist. in seven experiments, cardiac membranes from euthyroid rats bound 47 +/- 9 fmoles dhe/mg protein (mean +/- se) at saturation and demonstrated a dissociation constant (kd) of 2.5 +/- 0.4 nm. hyperthyroidism, produced by parenteral injection of triiodothyronine, significantly reduced the binding of dhe at all conc ... | 1979 | 94388 |
| genetic modulation of rna metabolism in drosophila. iv. measurement of rats of rna synthesis by density labeling. | accumulation of rna was measured in adult males of two genotypes: car bb/ybb- and car bb/ybbsuvar-5. the two genotypes have similar amounts of rdna, which is reduced in comparison to wild type (clark, strausbaugh and kiefer 1977). although genotypically bobbed, car bb/ybbsuvar-5 flies have a wild-type phenotype; car bb/ybb- flies are both phenotypically and genotypically bobbed (clark, strausbaugh and kiefer 1977). the wild-type phenotype observed in the car bb/ybbsuvar-5 flies is thought to be ... | 1979 | 94570 |
| pressor effect of beta-adrenergic receptor blocking drugs in spontaneously hypertensive rats treated with clonidine. | the opposing effects on blood pressure of clonidine and beta-adrenoceptor antagonists which have been observed in some hypertensive patients were studied in conscious, spontaneously hypertensive rats. daily treament with clonidine (100 micrograms/kg, given by gavage) for 2 weeks significantly reduced systolic blood pressure and heart rate. after 2 weeks of treatment with clonidine, administration by gavage of d,l-propranolol (10 mg/kg daily) or sotalol (10 mg/kg daily) or intraperitoneal injecti ... | 1979 | 94621 |
| [intracellular localization of alpha-fetoprotein and serum albumin in the central nervous system of the rat during fetal and postnatal development]. | the morphological localisation of alphafetoprotein (afp), serumalbumin (sa), transferrin and immunoglobulins (igg) has been studied in the developing central nervous system of the rat by immunocytochemical methods. evidence is presented of a highly selective staining for afp and sa, both proteins exhibiting the same topographical distribution. practically all the areas of the brain and the spinal cord are stained at a given moment of the developmental process. the labeling is cytoplasmic and in ... | 1979 | 95002 |
| some characteristics of the insulin-induced potentiation to anaphylactoid reaction in sprague-dawley rats. | the insulin-induced sensitization to generalized and local anaphylactoid reaction evoked by dextran was studied in sprague-dawley cfy rats. the generalized reaction was shown to be potentiated by insulin given subcutaneously in a dose-related manner. the minimum effective dose was as low as 0.04 u/kg. when this dose was injected intravenously, a marked but short-lived potentiation was observed. the insulin response could be elicited throughout the whole year. the local oedema induced by subplant ... | 1979 | 95468 |
| [immunological problems in oncology (author's transl)]. | the authors, after a view of the problems of immunology, regarding the most recent discoveries, deal with the conclusions about the immunological reactions in research and clinical oncology. many specific antibodies, formed as a result of the tumor, have been discovered in the field of research. it is impossible to say the same about human tumors and the probable hypothesis are numerous. the results until now obtained, even if they don't give a definite response, show that the immune defense of ... | 1979 | 95475 |
| a study of rabbit plasma progressive antithrombinic activity during acute phase inflammatory reaction and in experimental nephrotic syndrome. | 1. the development of the progressive antithrombinic activity in rabbit plasma as a function of the level of the two alpha-macroglobulins in two experimental pathologies: acute phase inflammatory reaction and nephrotic syndrome, were studied. 2. in the first case, the antithrombinic activity is a result of the increased biosynthesis of plasmatic antithrombins: antithrombin iii, alpha 1 antitrypsin and alpha macroglobulins. 3. in the nephrotic syndrome, this activity follows the increase in the a ... | 1979 | 95682 |
| deficiency of the ninth component of complement in man. | the studies of serum from a case with c9 (the ninth component of complement) deficiency are described. a 29-year-old woman in good health was found to have low serum complement levels (ch50). c9 of her serum was undetectable by the hemolytic assay and by the immunochemical analysis but all other components were normal. it was demonstrated that low ch50 of her serum was due to the hemolysis of the sensitized sheep erythrocytes (ea) by the complement components from c1 to c8. | 1979 | 95812 |
| [an experimental infection of hyalomma asiaticum and ornithodoros papillipes ticks with a single and combined infection with coxiella burnetii and dermacentroxenus sibericus]. | experimental infection of h. asiaticum and o. papillipes with coxiella burnetii and r. (d.) sibericus in different succession and individual study of these arthropods by means of the fluorescent antibodies method, ordinary microscopy and titration on laboratory animals have revealed an ambiguous outcome of the development of combined rickettsial infection in these ticks. the first agent obtained by the vector either prevents utterly the reproduction of the heterologous agent or inhibits its acti ... | 1979 | 95820 |
| naturally occurring diatomaceous pneumoconiosis in sub-human primates. | | 1978 | 96157 |
| [3h]serotonin release: an improved method to measure mast cell degranulation. | a method based on the release of tritium-labelled serotonin by activated mast cells in rodents is described. mast cells incorporate labelled serotonin selectively and release the label after activation by non-specific stimulators (compound 48/80, polymyxin b sulphate, atp, bovine chymotrypsin and l-alpha-lysophosphatidylcholine) or anaphylactic antibody and the corresponding antigen. these two types of activation were investigated in comparison with the toluidine blue microscopic rat mast cell d ... | 1978 | 96185 |
| [microsomal oxygenase enzyme system during chronic administration of 2-acetylaminofluorene]. | alteration in content of enzymatic components was studied in microsomal oxygenases system of rats administered with 2-acetyl aminofluorene. content of cytochrome r-450 was decreased beginning from the fourth week of the experiment. activity of nadp cytochrome c reductase was higher in animals, treated with the drug as compared with controls. inductors of the i and ii type were capable to induce the enzymatic system up to the end of the experiment - within 16 weeks as far as liver tumors develope ... | 1978 | 96595 |
| modification of vascular responsiveness to angiotensin ii in pregnant women by intravenously infused 5alpha-dihydroprogesterone. | in gravid women who are destined to develop pregnancy-induced hypertension (pih), normal pregnancy-associated refractoriness to the pressor effects of administered angiotensin ii (a-ii) is lost several weeks before the onset of hypertension. from a study of the determinants of a-ii pressor responsiveness in normal gravid women, it appears likely that the loss of resistance to a-ii is principally unrelated to plasma renin activity or to plasma a-ii levels. however, it recently has been shown that ... | 1978 | 96697 |
| susceptibility of human cultured cells to mason-pfizer monkey virus. | mason-pfizer monkey virus (m-pmv), originally isolated from mammary carcinoma of a rhesus monkey, is infectious to various human cells in tissue culture. human epithelioid cells (hela and hep-2), fibroblastic cells (embryonic cells as well as virus-transformed embryonic rsb cells), and glial cells (kc) have all been infected with m-pmv. electron microscope studies have shown that all infected cells produce progeny virus. the titer of infectious m-pmv in cultures of hela and hep-2 cells assayed b ... | 1978 | 97115 |
| nonrebreathing valve for spontaneously breathing or mechanically ventilated animals. | the design of a small dead space, nonbreathing valve suitable for use in small laboratory animals is presented. the design overcomes previous problems of condensation occurring at valve leaflets, and allows ongoing collection of expired gases should the animal require mechanical ventilation. the valve apparatus performs reliably when in operation for several hours at a time with a variety of small animals. | 1978 | 97251 |
| antigenic determinants of horse cytochrome c for lymphocyte transformation: identification of an immunodominant peptide. | | 1979 | 93078 |
| substance p infusion into substantia nigra of the rat: behavioural analysis and involvement of striatal dopamine. | the purpose of the present study was to characterize the nature of the behavioural response to substance p (sp) infusion into the substantia nigra and to evaluate this response in 6-hydroxydopamine (6-ohda) caudate lesioned rats. the effects of sp infusions (3 microgram in 1 microliter bilaterally) were assessed in an open field. two groups of rats were used: one with 6-ohda lesions in the caudate nucleus, and one with sham lesions. in sham rats, the first infusion produced a strong increase in ... | 1979 | 93550 |
| [effect of chlorfenvinfos and carbaryl on calcium and phosphorus metabolism in rat skeleton. ii. in vitro studies]. | | 1978 | 97769 |
| type-specific and non-type-specific reactions of purified m protein preparations. | m proteins of type 1 and type 12 streptococcus pyogenes were extracted by means of phage-associated lysin and purified by ion-exchange chromatography on cm and deae cellulose. molecular weight distributions were studied by gel chromatography on biogel a 0.5 m in a 6 molar urea solution and by sds electrophresis. serological activities were studied by the complement-fixation reaction and immunodiffusion and were compared with the estimated molecular weights. type-specific and non-specific activit ... | 1978 | 97870 |
| sensitivity of gonococcus strains isolated in rotterdam to epicillin, ampicillin, penicillin and tetracycline. | quantitative determinations of the sensitivity to epicillin, ampicillin, penicillin and tetracycline were carried out for 67 gonococcus strains isolated from 67 males with urogenital gonorrhoea, and 38 strains isolated from 38 females with urogenital/rectal gonorrhoea. the results of the sensitivity determinations for ampicillin, penicillin and tetracycline were compared with the findings of a previous investigation. it was found that the gonococcus strains isolated from males had become slightl ... | 1978 | 98362 |
| observations on acute gastric dilatation in nonhuman primates. | in the years 1967-1977 we diagnosed 23 cases of acute gastric dilatation in monkeys. fourteen of these animals were macaca mulatta, five macaca fascicularis, and one each of macaca nemestrina, aotus trivirgatus, saimiri sciureus, and colobus guereza. fourteen of the animals were males, nine were females, and all were adults or subadults. mortality was 78% (18 of 23 animals). thirteen of the animals had received on anesthetic, immobilizing, or tranquilizing drug 1-2 days before developing acute g ... | 1978 | 98666 |
| jaundice and breast-feeding among alaskan eskimo newborns. | the course, incidence, and severity of neonatal jaundice was studied in 95 alaskan eskimo infants. breast-fed infants had higher bilirubin concentrations than bottle-fed babies. both groups experienced high bilirubin levels, similar to those previously reported in navajo and oriental infants but greater than those observed in whites and blacks. a marked capacity to inhibit hepatic glucuronyl transferase was observed in breast-milk specimens but only partly accounted for the bilirubin differences ... | 1978 | 99026 |
| some ultrastructural effects of persistent infections by the rickettsia coxiella burnetii in mouse l cells and green monkey kidney (vero) cells. | mouse fibroblasts (l-929) and vero (green monkey kidney) cells were infected with the rickettsia coxiella burnetti, and persistent infections developed and were studied over a 6- to 10-month period. ultrastructural comparisons were made between the two infected cell types, and both were tested cytochemically for the presence of acid phosphatase, a marker enzyme of lysozymes. rickettsiae were always observed within vacuoles, and some infected l cells showed flattened endoplasmic reticulum as comp ... | 1978 | 99368 |
| one-carbon metabolism in methanogenic bacteria: analysis of short-term fixation products of 14co2 and 14ch3oh incorporated into whole cells. | methanobacterium thermoautotrophicum, m. ruminantium, and methanosarcina barkeri were labeled with 14co2 (14co2 + h14co3- + 14co32-) for from 2 to 45 s. radioactivity was recovered in coenzyme m derivatives, alanine, aspartate, glutamate, and several unidentified compounds. the properties of one important structurally unidentified intermediate (yellow fluorescent compound) displayed uv absorbance maxima at ph 1 of 290 and 335 nm, no absorbance in the visible region, and a fluorescence maximum at ... | 1978 | 101522 |
| effect of nitroglycerin on peripheral blood flow distribution and venous return. | | 1978 | 101653 |
| crystallographic data on a complete kappa-type human bence-jones protein. | | 1978 | 101675 |
| the response of the living organism to dead and fixed dead enclosed homologous tissue. | polyethylene tubes at both ends and with four perforations in the side walls were filled with untreated homologous necrotic muscle tissue or formaldehyde-fixed or glutaraldehyde-fixed homologous necrotic tissue and implanted subcutaneously in rats. the cell reaction in the tissues surrounding the differently filled tubes was examined. it was found that the inflammatory reaction to untreated necrotic homologous tissue was the least severe. | 1978 | 101928 |
| renal effects of repeated administration of calcium disodium ethylenediaminetetraacetate during excessive exposure to lead in rats. | the renal effects of repeated administration of edta were studied in rats given 0.5% lead as lead acetate in drinking water for 24 weeks. repeated treatment of lead toxic rats with edta during continuous exposure to lead reduced blood lead levels and brain and bone lead but not to a statistically significant level (p greater than 0.05). liver lead concentration in the edta treated, lead exposed rats changed only slightly upward, but kidney lead levels decreased greatly when compared to lead expo ... | 1978 | 102714 |
| insulin counteraction of alpha-adrenergic effects on liver glycogen metabolism. | insulin counteracted the effects of a pure alpha-adrenergic agonist, phenylephrine, on hepatocyte glycogen synthase and phosphorylase. these results argue against current concepts of insulin increasing cytoplasmic ca2+ concentration. | 1978 | 103580 |
| restricted posterior parietal lesions in the rhesus monkey and performance on visuospatial tasks. | monkeys with lesions restricted to the inferior parietal lobule or the banks and depths of the superior temporal sulcus were tested on a route-following task. these areas are considered on neuroanatomical grounds to be homologous to parts of the human posterior parietal cortex, where lesions produce profound spatial disorientation. the operated monkeys were impaired on the route task, thus confirming at the behavioural level the anatomical predictions of comparability between parietal cortex in ... | 1979 | 103604 |
| [boutonneuse fever in tourists as a model for clinical diagnosis of rickettsioses (author's transl)]. | case histories of boutonneuse fever are described in order to exemplify major characteristics of most rickettsioses: recent travel history, feverish illness with severe headache, skin eruptions and histological findings. up-to-date informations concerning the epidemiologic situation of typhus, scrub typhus and rocky mountain spotted fever are given. the characteristics of q fever and the possibility of rickettsial laboratory infections are pointed out. | 1979 | 104166 |
| [inhibition of lh-rh induced release of gonadotropins by substance p in cultured rat anterior pituitary cells]. | using anterior pituitary cells cultured for 7 days and then incubated for 4 hrs, substance p, an undecapeptide, inhibited the stimulatory effect of lh-rh on the release of lh-rh on the release of lh and fsh. this inhibitory effect, which was similar for both gonadotropins was only observed when the adenopituitary cells were put in culture at di and proestrus stages of the oestrous cycle. furthermore substance p partly inhibited the basal release of fsh at di and dii stages but did never affected ... | 1978 | 104797 |
| is gip a glucagon cell constituent? | "gastric inhibitory peptide" or "glucose-dependent insulin-releasing peptide" (gip) is a member of the gut hormone family. its physiological action is thought to be related to its insulinotrophic effect. the occurrence and distribution of gip was studied by immunohistochemistry. in all species examined including man, gip immunoreactivity was found to reside in the glucagon cells of the pancrease and gut. three pancreatic glucagonomas were found to contain numerous cells displaying gip and glucag ... | 1978 | 104936 |
| ultrastructure and cytochemical localization of laccase in two strains of leptosphaerulina briosiana (pollaci) graham and luttrell. | substrate specificity tests were used to identify the presence of laccase in two strains of leptosphaerulina briosiana (poll.) graham and luttrell, an ascomycete which causes leaf spot in alfalfa. cytochemical localization of monophenol monooxygenase (laccase) as well as the ultrastructures of the two strains were investigated. laccase was observed in the outer layers of the cell walls of both strains. the ultrastructures of vegetative hyphae of both strains were typical of those found in most a ... | 1979 | 104971 |
| monkey brain arylamidase. ii. further characterization and studies on mode of hydrolysis of physiologically active peptides. | a large-scale purification of monkey brain arylamidase was carried out. amino acid analyses indicate that the enzyme is rich in acidic amino acids and is poor in cystine. the amino terminal residue was determined to be alanine by dansylation. the enzyme was activated by sulfhydryl compounds. dithiothreitol was more effective than beta-mercaptoethanol. bestatin competitively inhibited the enzyme activity and the ki value was calculated to be 2.5 x 10(-7) m, which was of the same order as that of ... | 1978 | 104979 |
| aspirin inhibits development of coronary atherosclerosis in cynomolgus monkeys (macaca fascicularis) fed an atherogenic diet. | the effect of aspirin in the primary prevention of diet-induced atherogenesis in cynomolgus monkeys was studied. the diet consisted of 2% cholesterol and 10% butter by weight for 24 wk. six monkeys received only the atherogenic diet and five monkeys received the diet plus aspirin, 81 mg/monkey per day. aspirin did not affect plasma cholesterol levels or aortic atherosclerosis. platelet aggregation to arachidonic acid was almost completely suppressed. aspirin decreased significantly the number of ... | 1979 | 105014 |
| a serologic survey of mule deer and elk in utah. | sera from mule deer (odocoileus hemionus) and elk (cervus canadensis) in central and northern utah were tested for the prevalence of antibodies to 11 diseases communicable to man or domestic livestock. antibodies to francisella tularensis (at 1:20) were found in 47 of 88 (53.4%) elk and 1 of 89 (1.1%) deer. a screening slide agglutination test for titers to brucella (at 1:20) showed two reactors in elk but none in deer sera. no positive antibody titers were obtained in tests for anaplasmosis, co ... | 1978 | 105153 |
| beta-galactosidase chimeras: primary structure of a lac repressor-beta-galactosidase protein. | a protein possessing both lac repressor and beta-galactosidase activities in a single polypeptide of about 155,000 daltons was purified from a deletion mutant of escherichia coli in which the laci and z genes are fused. a 77-residue cyanogen bromide peptide containing the fusion joint was isolated. a radioimmunoassay with an antibody prepared against cnbr2 (residues 3-92) of beta-galactosidase was used to monitor its purification. the sequence of the joining peptide was determined by analysis of ... | 1978 | 105358 |
| the effects of a mixture of mestranol and ethynerone on the female monkey. | | 1979 | 105931 |
| comparative study of aggressive behaviour after injection of cholinomimetics, anticholinesterases, nicotinic, and muscarinic ganglionic stimulants into the cerebral ventricles of conscious cats: failure of nicotinic drugs to evoke aggression. | | 1979 | 106424 |
| [cattle vaccination against q fever in the rural district of bratislava]. | cattle attendants on two farms in the outer bratislava district showed symptoms of q-fever. the blood of the cows in these farms was found to contain antibodies to coxiella burnetti and the causative agent of the disease was detected in milk samples of aborting cows by biological assays on hamsters (mesocricetus auratus). the naturally invaded dairy cows with antibodies in the blood and heifers without antibodies were vaccinated with different doses of inactivated suspension of c. burnetii in st ... | 1978 | 106503 |
| [accelerated diagnostic cultivation of pathogenic environmental microbes. ii. accelerated diagnostic cultivation of the causative agents of anthropozoonotic plague, glamders, melioidosis, brucellosis and tularemia]. | | 1978 | 106632 |
| renal emphysema of the tranplanted kidney: sonographic appearance. | | 1979 | 106703 |
| isolation of listeria monocytogenes from animals in the sudan. | | 1979 | 107996 |
| cynomolgus monkey model for experimental q fever infection. | a subhuman primate model was developed for study of the pathogenesis of infection with coxiella burnetii. cynomolgus monkeys (macaca fascicularis) that were exposed to 10(5) mouse median infectious intraperitoneal doses of c. burnetii in a small-particle aerosol developed clinical signs of illness and pathologic changes characteristic of q fever infection in humans. all monkeys had radiologic evidence of pneumonia by day 9. antibodies to c. burnetii were detectable by the indirect fluorescent an ... | 1979 | 108342 |
| ["banal" virus infections. what should be done?]. | | 1979 | 108538 |
| polydipsia in the monkey generated by visual display schedules. | | 1979 | 108693 |
| piracetam facilitates retrieval but does not impair extinction of bar-pressing in rats. | rats were trained on a continuously reinforced bar-press response for water reward. seven days later they were retested for retention, with or without pretest injection of the nootropic drug, piracetam. drug-treated animals had significantly shorter response latencies than saline-treated animals. the results are interpreted as a facilitation of retrieval processes after forgetting. the experiment was extended under extinction conditions and it was found that after three sessions there was a tend ... | 1979 | 108722 |
| sex differences in behavioral and thermal responses to pargyline and tryptophan. | the effects of parenterally injected pargyline and tryptophan on rectal temperature and behavior have been studied in male and female rats. pargyline alone (50 mg/kg) produced hypothermia in both sexes. pargyline (50 mg/kg) followed by low doses (20--50 mg/kg) of tryptophan caused a behavioral syndrome consisting of tremor, hindlimb abduction, forepaw treading, and straub tail. in females, but not in males, hypothermia was potentiated. the same dose of pargyline followed by higher doses (60--150 ... | 1979 | 108723 |
| comparison of tolerance development and dependence capacities of morphine, beta-endorphin, and [d-met2, pro5]-enkephalinamide. | the tolerance-development capacities of beta-endorphin, [d-met2, pro5]-enkephalinamide, and morphine were compared in rats, and the dependence capacity of morphine was compared with that of the enkephalin analogue in mice. tolerance to the analgesic effect, as measured by the tail-flick test, developed somewhat more rapidly in the [d-met2, pro5]-enkephalinamide-treated group than in the others. a similar relationship was found for the dependence capacity. considering that the enkephalin analogue ... | 1979 | 108745 |
| effect of drugs influencing central serotonergic mechanisms on haloperidol-induced catalepsy. | pretreatment with quipazine, a serotonin agonist, and clomipramine, a selective serotonin neuronal uptake blocker, was found to potentiate the cataleptic effect of haloperidol in a dose-dependent manner in rats. pretreatment with methysergide, a serotonin antagonist, reduced the cataleptic effect of haloperidol. the results indicate that the cataleptic effect of neuroleptics depends on the balance between the dopaminergic and serotonergic systems, and that the serotonergic system exerts an inhib ... | 1979 | 108749 |
| restriction cleavage map of sp01 dna: general location of early, middle, and late genes. | a detailed restriction endonuclease map for the genome of bacillus subtilis phage sp01 is presented. sites of cleavage for the restriction enzymes bglii, ecori, haeiii, and sali were determined. this physical map showed that sp01 dna was 140 kilobases in length and contained a repeated sequence of 12.4 kilobases at its termini. combined with previously published information, we were also able to identify the general locations of genes expressed at early, middle, or late times in the phage lytic ... | 1979 | 116010 |
| suppression of pha-stimulated lymphocyte transformation in cynomolgus monkeys following infection with coxiella burnetii. | phytohemagglutin (pha) induced blastogenesis of peripheral blood lymphocytes from cynomolgus monkeys infected with c. burnetii was suppressed between 14 and 28 days after infection. lymphocytes became responsive to pha again on day 35 with an increase in stimulation index when cultured with specific antigens. in contrast, production of specific humoral antibodies was not diminished during the acute and early convalescent stages of infection. | 1979 | 118801 |
| comparison of 15n flux and nitrogen balance with carcass analysis during oral and iv nutrition. | | 1979 | 120030 |
| monkey pulpal response to composite resin restorations in cavities treated with various cleansing agents. | pulpal response to composite resin restorations placed in experimentally prepared cavities treated with various cavity cleansing and etching agents were studied in monkey teeth. the use of etching agents and cleansers with a demineralizing potential enhanced the pulpal response to composite resin restorations. bacterial proliferation on the cavity walls around the composite restorations may be one of several factors causing the harmful pulpal responses found. the protective effect of a calcium h ... | 1979 | 120607 |
| glomerulonephritis associated with coxiella burnetii endocarditis. | a patient with endocarditis associated with chronic coxiella burnetii infection is described in whom glomerulonephritis developed with granular deposits containing immunoglobulins and complement in the glomeruli. the serum was notable for the variety of circulating antibodies detected, which included antibodies directed against native dna. | 1975 | 123164 |
| rb86 uptake of various organs of the spontaneously hypertensive rats: (a preliminary note). | twenty spontaneously hypertensive rats (shr) and 20 control rats were used for measurements of rb86 uptake of various organs. hemodynamic measurements and the heart weight to body weight ratio showed a significant and sustained hypertension and increased heart rate in shr; ratio of rb86 uptake in the kidney, brain, liver, adrenal gland, pancreas, and spleen to that in rv was smaller in shr than that in the control rats. the ratio of rb86 uptake of inner layer to outer layer of lv, which represen ... | 1975 | 123284 |
| fatal immune complex glomerulonephritis without deposits. | repeated intravenous injections of egg albumin in rabbits produced small antigen-excess complexes and severe glomerulonephritis. immunoglobulins and complement in the glomeruli were not clearly demonstrated by immunofluorescence; deposits were found to be sparse by electron microscopy. this study demonstrates that soluble immune complexes are responsible for the glomerular reaction. the apparent absence of deposits is thus not sufficient to exclude an immune complex pathogenesis. | 1975 | 123971 |