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immunization against q-fever of naturally infected dairy cows.dairy cows infected naturally with coxiella burnetii as evidenced either by presence of phase ii agglutinating antibodies in the blood or by shedding c. burnetii in the milk, were vaccinated subcutaneously with formalin-killed phase i c. burnetii organisms. attempts to demonstrate c. burnetii in the milk of vaccinated dairy cows 47 days after vaccination were negative, while continuous shedding of c. burnetii in the milk of control non-vaccinated dairy cows was repeatedly demonstrated in the cou ...19751994
selection of chlortetracycline-resistant strain of coxiella burnetii. 19751997
precipitation of phase i antigen of coxiella burnetii by sodium sulfite. 19752000
characterization of an endotoxic lipopolysaccharide from coxiella burnetii.phase i coxiella burnetii antigen isolated by phenol extraction from purified suspensions of c. burnetii in phase i is a complex lipopolysaccharide (lps) molecule containing substances typical of the bacterial lps. some endotoxic properties of this c. burnetii lps, namely pyrogenicity and skin epinephrine reaction in rabbits, hypothermia in white rats, lethal effect on chicken embryos or on actinomycin-d-treated mice are similar to those of lps isolated from other gram-negative bacteria.19765871
sedimentation of phase ii coxiella burnetti strains in cscl density gradient. 19769810
detection of complement-fixing antibodies against coxiella bunetii in sera of experimentally infected hens. 19769814
analysis of antibody response and immunoglobulins in sera of rabbits and guinea pigs immunized with coxiella burnetii.rabbit and guinea pig sera and their immunoglobulin fractions (igm and igg) were examined by complement-fixation (cf), microagglutination (ma), opsonization-phagocytosis (op) and serum protection (sp) tests at intervals after immunization with live phase i and phase ii coxiella burnetii suspensions. in general, ma antibodies, but also decreased, earlier than cf antibodies. the anamnestic immune response was higher with lower primary doses. both phase ii and phase i cf and ma antibodies as well a ...197718924
search for correlates of resistance to virulent challenge in mice immunized with coxiella burnetii.mice immunized with live phase i or phase ii coxiella burnetii, with killed phase i or phase ii organisms or with trichloroacetic acid (tcae) or phenol (pe) extracts were resistant to intraperitoneal infection with phase i c. burnetii irrespective of whether or not they displayed phase i antibody response at the time of virulent challenge. increased phagocytosis of purified phase i organisms by blood leukocytes or peritoneal exudate cells (pec) was noticed only in mice with phase i agglutinating ...197722238
seroepidemiological investigations in domestic ruminants from egypt, somalia and jordan for the demonstration of complement fixing antibodies against rickettsia and chlamydia (author's transl).1450 random serum samples of domestic ruminants from egypt, somalia and jordan were investigated for complement fixing antibodies against rickettsia and chlamydia. between 1.5 and 3.4% of the samples from the animals investigated had antibodies against the rmsf-group of rickettsia, with exception of the sera from somalian cattle and sheep from jordan. antibodies against rickettsia of the typhus-group were found in 4 cattle and 1 goat from jordan and 2 sheep from egypt; by agglutination test with ...197828651
influence of mild acid hydrolysis on the antigenic properties of phase i coxiella burnetii.mild acid hydrolysis of phase i coxiella burnetii cells gradually cleaved off polysaccharide chains, which contain determinants of antigen 1, thus unmasking determinants of the diagnostically important antigen 2. this artificial phase i to phase ii conversion was reflected by changed serological activity in the complement-fixation (cf) test and by changed nonspecific phagocytosis. the product of mild acid hydrolysis can serve as a complex diagnostic preparation, because it reacts in the cf test ...197829469
immunological properties of the lipopolysaccharide-protein complex of coxiella burnetii.purified lipopolysaccharide-protein complex (lps-pc) extracted by trichloroacetic acid from phase i coxiella burnetii organisms induced in mice and rabbits fair levels of antibodies directed to antigen 1 and antigen 2, as detected by complement-fixation (cf), microagglutination (ma), opsonization-phagocytosis (op) and serum protection (sp) tests. in guinea pigs only very low levels of ma antibodies against antigen 2 were demonstrated. in rabbit serum, ma antibodies directed to antigen 2 were fou ...197829470
experimental infection of hare (lepus europaeus) with coxiella burnetii and rickettsia slovaca.hares (lepus europaeus l.) infected subcutaneously (s.c.) or per os with coxiella burnetii and s.c. with rickettsia slovaca overcame an inapparent infection accompanied by irregular rickettsaemia and distribution of rickettsiae to different organs and variable antibody response. neither riskettsia could be detected in faeces of infected animals, but c. burnetii was found in the urine of one hare, which died on day 13 post infection (p.i.). antibodies against c. burnetii persisted for one year of ...197830267
attempts at demonstration of lipopolysaccharide in phase ii coxiella burnetii.comparison of some properties of phase i and phase ii coxiella burnetii cells suggests the presence of lipopolysaccharide (lps) also in the surface structures of phase ii cells. polysaccharide chains were released from them by mild acid hydrolysis and corpuscular residues resulting from such hydrolysis elicited in rabbits anti-lipid a-antibodies. toxicity for adrenalectomized and actinomycin d-sensitized mice was demonstrated with phase i cells, but not with much higher concentrations of phase i ...197835951
antibody response in man following a small intradermal inoculation with coxiella burnetii phase i vaccine.a small inoculum (0.2 microgram) of phase i coxiella burnetii vaccine given to individuals previously sensitized to co burnetii elicited a positive skin reaction and a strong igm phase i antibody response as determined by microagglutination, complement fixation and microimmunofluorescence tests. a similar inoculum administered to nonsensitized individuals did not elitic a skin reaction nor stimulate a recognizable antibody response. serum from one of these sensitized and skin tested individuals ...197935962
isolation of endotoxic lipopolysaccharide from phase ii coxiella burnetti. 197940425
[new aspects of the part of the vector played by ixodes ricinus l. in switzerland. preliminary note (author's transl)].the authors, after having recalled their recent work on ixodes ricinus ecology, give the new results about the part played by this species in the transmission of different infectious agents in switzerland. i. ricinus was already known to be the most important vector of the tick borne encephalitis virus, and of protozoans of the babesia genus. in this article, we describe the existence in the hemolymphe of different i. ricinus populations, of a rickettsia species related to the rmst group (rocky- ...197941427
inhibition by coxiella burnetii of ascites tumour formation in mice.mice injected intraperitoneally (i.p.) with killed purified coxiella burnetii organisms were protected from ascites development and death caused by i.p. inoculation of sarcoma-180 cells. the extent of protection was a function of the relative dose of c. burnetii and tumour cells, and of the time of injection of c. burnetii. phase ii c. burnetii organisms exerted an antitumour protection at least as high as phase i c. burnetii organisms.197941443
suitability of coxiella burnetii strains for preparation of phase ii artificial diagnostic antigen for microagglutination test.sensitivity in the microagglutination (ma) test of artificial (prepared by potassium periodate treatment) phase ii coxiella burnetii antigens depended on the c. burnetii strain used and the number of its chick embryo yolk sac passages.197942306
electron microscopy studies of the limiting layers of the rickettsia coxiella burneti.surface layers of coxiella burneti studied at a high resoulution reveal a plasma membrane and an outer surface membrane 6 to 7 nm thick, and a thin, moderately electron-dense intermediate layer associated with the inner surface of the outer membrane of many cells. this layer appears to be unaffected by lysozyme treatment. ruthenium red staining was used to delineate a layer of filamentous material external to the outer membrane; this fuzzy layer has a mean thickness of 20 nm and is not often see ...197547324
preparing and staining of coxiella burnetii natural phase ii antigen for the microagglutination reaction.an improved method of preparing coxiella burnetii natural phase ii antigen of high purity enabled its staining for use in the microagglutination reaction (mar). the antigen was as specific and sensitive for detection of phase ii antibodies as the artificial phase ii c. burnetii antigen prepared by periodate treatment from purified phase i cells of c. burnetii.197661717
comparison of different antigenic preparations of coxiella burnetii used for antibody detection in guinea pigs.the dynamics of antibody response in guinea pigs infected with coxiella burnetii was investigated by microagglutination (ma) and complement-fixation (cf) tests with different preparations of c. burnetii antigens. at the onset of antibody response the highest antibody titres were detected by the ma test with natural antigen 2, later on by the ma test with artificial antigen 2. throughtout the 1-year period of observation, the cf antibody levels were usually lower and, with the exception of the hi ...197887123
simple, differential staining technique for enumerating rickettsiae in yolk sac, tissue culture extracts, or purified suspensions.a differential staining method employing acridine orange-stained rickettsiae and safranin-stained standardized suspensions of shigella dysenteriae is described for enumerating rickettsiae harvested during various stages of the growth cycle.197988460
chronic cryptic q-fever infection of the heart.evidence of chronic coxiella burneti infection of the heart, a disease previously considered peculiar to patients with valvular heart-disease, was found in a patient during routine serological tests before resection of a ventricular aneurysm and also by isolation of the rickettsia from the resected tissue. the patient had no symptoms or signs of q-fever endocarditis and none of the laboratory evidence usually associated with it.197988607
[determination of igm- and igg-antibodies to coxiella burnetii]. 197994131
[an experimental infection of hyalomma asiaticum and ornithodoros papillipes ticks with a single and combined infection with coxiella burnetii and dermacentroxenus sibericus].experimental infection of h. asiaticum and o. papillipes with coxiella burnetii and r. (d.) sibericus in different succession and individual study of these arthropods by means of the fluorescent antibodies method, ordinary microscopy and titration on laboratory animals have revealed an ambiguous outcome of the development of combined rickettsial infection in these ticks. the first agent obtained by the vector either prevents utterly the reproduction of the heterologous agent or inhibits its acti ...197995820
some ultrastructural effects of persistent infections by the rickettsia coxiella burnetii in mouse l cells and green monkey kidney (vero) cells.mouse fibroblasts (l-929) and vero (green monkey kidney) cells were infected with the rickettsia coxiella burnetti, and persistent infections developed and were studied over a 6- to 10-month period. ultrastructural comparisons were made between the two infected cell types, and both were tested cytochemically for the presence of acid phosphatase, a marker enzyme of lysozymes. rickettsiae were always observed within vacuoles, and some infected l cells showed flattened endoplasmic reticulum as comp ...197899368
[cattle vaccination against q fever in the rural district of bratislava].cattle attendants on two farms in the outer bratislava district showed symptoms of q-fever. the blood of the cows in these farms was found to contain antibodies to coxiella burnetti and the causative agent of the disease was detected in milk samples of aborting cows by biological assays on hamsters (mesocricetus auratus). the naturally invaded dairy cows with antibodies in the blood and heifers without antibodies were vaccinated with different doses of inactivated suspension of c. burnetii in st ...1978106503
cynomolgus monkey model for experimental q fever infection.a subhuman primate model was developed for study of the pathogenesis of infection with coxiella burnetii. cynomolgus monkeys (macaca fascicularis) that were exposed to 10(5) mouse median infectious intraperitoneal doses of c. burnetii in a small-particle aerosol developed clinical signs of illness and pathologic changes characteristic of q fever infection in humans. all monkeys had radiologic evidence of pneumonia by day 9. antibodies to c. burnetii were detectable by the indirect fluorescent an ...1979108342
[importance of humoral immunity indices in determining the rickettsia prowazekii carrier state].experiments showed the possibility of making indirect conclusions concerning rickettsial carrier state by the method of determination of complement-fixing antibodies to r. prowazeki in the blood serum. though not indicative of carrier state in individual animals, these antibodies, their dynamics and titers gave the evidence of group carrier state in cotton rats in respect of the causative agent of typhus. the number of animals carrying r. prowazeki increased with the rise of antibody titers. neg ...1979110016
suppression of pha-stimulated lymphocyte transformation in cynomolgus monkeys following infection with coxiella burnetii.phytohemagglutin (pha) induced blastogenesis of peripheral blood lymphocytes from cynomolgus monkeys infected with c. burnetii was suppressed between 14 and 28 days after infection. lymphocytes became responsive to pha again on day 35 with an increase in stimulation index when cultured with specific antigens. in contrast, production of specific humoral antibodies was not diminished during the acute and early convalescent stages of infection.1979118801
[lipopolysaccharides of rickettsiaceae and the limulus endotoxin assay].the a.a. have examined by limulus endotoxin assay some lps preparations from r. typhi, r. slovaka, c. burnetti phase i and ii, as a demonstration of endotoxicity. all the preparations tested were able to gel the limulus amoebocyte lysates, even if to a different degree of reactivity. the results add credibility to the hypothesis that lps from rickettsiaceae can represent at least in part the pathogenetic mediators of some manifestations described in rickettsiosis; however, without endotoxin assa ...1979121982
glomerulonephritis associated with coxiella burnetii endocarditis.a patient with endocarditis associated with chronic coxiella burnetii infection is described in whom glomerulonephritis developed with granular deposits containing immunoglobulins and complement in the glomeruli. the serum was notable for the variety of circulating antibodies detected, which included antibodies directed against native dna.1975123164
[prevalence of q fever in czechoslovakia (author's transl)]. 1976132287
rickettsioses studies. 1. natural foci of rickettsioses in the armenian soviet socialist republic.ixodid ticks collected in the armenian ssr during 1971 and 1974 were positive for rickettsiae of the spotted fever group, as confirmed by haemocyte tests and by isolation experiments. serum specimens collected from human beings and from domestic and wild animals in the same areas contained antibodies against such rickettsiae and against coxiella burnetii. these results indicate the existence of mixed natural foci of rickettsioses of the spotted fever group and of q fever in the armenian ssr.1976134846
host response to infection by coxiella burneti.a host response to infection by coxiella burneti was investigated. infectedyolk sacs were harvested from embryonated eggs and assayed for glycolytic activity. assays of glycolytic enzymes included glucose isomerase, aldolase, phosphofructokinase,fructose-1,6-diphoshatase, glyceraldehyde-3-phosphate dehydrogenase, enolase, and pyruvate kinase. no significant differences in enzymatic activity between normal and infected tissues through the 12th day was observed. from the 13th day through the 16thd ...1975164999
a review of routine tests for respiratory viruses in hospital inpatients.during the period june 1967 to may 1972, viral tests were performed upon 1195 inpatients aged 12 and over in the brompton hospital. the overall diagnostic rate was 21-5%, comparing 9-3% by isolation and 14-9% by complement fixation (6-5% by four-fold rises in titre in paired sera, 8-4% by titres larger than or equal to 160 in single sera). only 42% of all patients had an acute respiratory illness within one week prior to admission, which seriously curtailed the chances of isolating viruses and o ...1975170701
[atypical pneumonia, etiology and possibilities for the diagnosis (author's transl)].beginning with the antimicrobial chemotherapy a decrease in the incidence of bacterial pneumonias is accompanied by a relative increase in the incidence of the so-called atypical pneumonia. this disease syndrome is predominantly caused by mycoplasma pneumoniae, chlamydia psittaci, coxiella burneti and various viruses. in addition, bacteria which are usually involved in lobar pneumonia may occasionally cause atypical pneumonias. the present publication is concerned with the most frequently occurr ...1976186398
synthesis of ribonucleotides and their participation in ribonucleic acid synthesis by coxiella burnetii.synthesis of ribonucleic acid (rna) by the deoxyribonucleic acid-dependent rna polymerase of coxiella burnetii required adenosine, uridine, guanosine, and cytidine 5'-triphosphates. cell-free preparations of this obligate intracellular procaryotic parasite had competence to phosphorylate ribonucleoside mono- and diphosphates in the presence of exogenous adenosine and guanosine 5'-triphosphates to the corresponding di- and triphosphates. c. burnetii contained about 2 nmol of adenosine 5'-triphosp ...1977200603
[fluorescent fab-fragments of antibodies. comparative assessment and utilization in immunofluorescent analysis].the authors present the results of a comparative study of the immunofluorescent activity and the specificity of fluorescent fab-fragments of antibodies to r. prowazeki, d. sibericus, and b. pertussis obtained by the enzymatic hydrolysis of specific immunoglobulins with papain. fluorescent fab-fragments of antibodies possessed the same sensitivity and specificity as the homologous fluorescent antibodies, but had an advantage of a much weaker capacity to nonspecific fluorescence and a relatively h ...1977203147
changes in liver and l-cell plasma membranes during infection with coxiella burnetii.changes in plasma membrane proteins of guinea pig liver and l-929 cells were studied during infection with coxiella burnetii. polypeptide species resolved by disc polyacrylamide gel electrophoresis with sodium dodecyl sulfate showed quantitative but no qualitative differences between uninfected and infected samples. when the o'farrell technique of isoelectric focusing, followed by sodium dodecyl sulfate-slab gel polyacrylamide gel electrophoresis, was employed, additional polypeptides were resol ...1978203534
[mononucleosis syndrome and thrombocytopenic purpura in q fever (rickettsia burneti)].case of a patient with a mononucleosis syndrome and thrombocytopenic purpura during q fever. this etiology could lead to a systematic search for a rickettsial cause for such a symptomatology with a negative paul-bunnell-davidson reaction.1978211604
erythrocyte-sensitizing substance from rickettsia canadia.erythrocyte-sensitizing substance (ess) extracted from rickettsia canada reacted with homologous sera as well as with sera containing antibodies against rickettsia prowazeki, but did not react with sera containing antibodies against rickettsia conori or coxiella burneti. this finding confirms the antigenic relatedness of r. canada to the typhus rickettsiae group.1975239581
phase ii to phase i conversion of coxiella burneti in immunosuppressed mice.coxiella burneti strains 48 and nine mile, serologically in phase ii, changed their antigenic properties and virulence for guinea pigs and mice upon passaging in immunosuppressed mice. conversion to phase i of these strains in a system lacking antibody response throws doubt on the importance of specific antibodies in phase variation of c. burneti.1975241236
phagocytosis of coxiella burneti by macrophages.live or killed purified phase i and phase ii coxiella burneti organisms were phagocytized to a similar extent by mouse or guinea pig peritoneal macrophages and polymorphonuclear leukocytes (pmn); phase ii was much more susceptible to phagocytosis than phase i. phagocytosis of phase i was enhanced only by immune sera containing phase i antibodies. increased in vivo phagocytosis of phase i organisms by macrophages from animals immunized with live phase i c. burneti was lost following their in vitr ...1975241247
rickettsioses studies. 3. natural foci of rickettsioses in south bohemia.antibodies against coxiella burnetii and against rickettsiae of the spotted fever group were found in human sera and in sera from domestic and wild animals collected in south bohemia. spotted fever group rickettsiae were also discovered in the tick ixodes ricinus. these results indicate the presence of both types of rickettsiae in this part of czechoslovakia. as no epizootics or epidemics of q fever have as yet been reported in the area, it can be assumed that c. burnetii occurs in the latent st ...1977304389
[behavior of the q fever vector coxiella burnetii in birds. 2. experimental infection of pigeons]. 1977319989
[behavior of the q fever vector, coxiella burnetii, in birds. 1. natural infections in birds]. 1977319990
appearance of cellular and humoral immunity in guinea pigs after infection with coxiella burnetii administered in small-particle aerosols.the development of humoral and cell-mediated immune responses was studied in guinea pigs infected with coxiella burnetti administered in small-particle aerosols. direct macrophage migration inhibition was observed in cultured peritoneal exudate cells as early as 3 days after exposure. maximum inhibition of macrophages cultured with phase i or ii antigen occurred 14 to 21 days postexposure and persisted through 35 days. this inhibitory action was no longer detectable at 42 days. serum antibody to ...1977324911
[the behavior of the q-fever agent, coxiella burnetii, in birds. 3. experimental infection of quail].after simultaneous aerogenic and alimentary infection of quail by intra-nasal inoculation of a suspension of c. burnetii, the agent was reisolated 6 h after infection from lung and gut, from the 3rd day on from the spleen, at the 8th and 10th day from blood, and from the 8th day on from liver and kidney. c.burnetii was found in various organs up to 21 days after infection. in the majority of birds agglutinating antibodies could be demonstrated from the 18th day up to termination of the experimen ...1977334506
comparison of ribosomes from coxiella burnetii and escherichia coli by gel electrophoresis, protein synthesis, and immunological techniques.ribosomes and postribiosomal supernatant fluid (s-100) were isolated from coxiella burnetii. the ribosomes functioned in polyuridylic acid-directed polyphenylalanine synthesis in the presence of s-100 from either c. burnetii or escherichia coli. c. burnetii s-100 promoted translation with e. coli ribosomes. antisera against e. coli elongation factor g and ribosomal proteins l7/l12 cross-reacted with rickettsial s-100 and ribosomes, respectively. ribosomal proteins were analyzed by two-dimensiona ...1978361701
military significance of q fever: a review. 1978361954
resistance to babesia spp. and plasmodium sp. in mice pretreated with an extract of coxiella burnetii.mice injected intravenously with a commercially available extract of coxiella burnetii prepared for use as the antigen in the complement fixation diagnostic test for q fever were subsequently resistant to infection with babesia microti, babesia rodhaini, and plasmodium vinckei petteri. the parasites appeared to die inside circulating erythrocytes. protection was unaffected by exposing the pretreated mice to 900 rads on the day before they were infected. to explain these findings, it is postulate ...1979378850
activation of guinea pig macrophages by q fever rickettsiae. 1977401688
differences in buoyant-density properties of coxiella burnetii and rickettsia rickettsii.the pronounced change in the buoyant density of coxiella burnetii in cscl gradients that was caused by treatment with formalin or ultraviolet radiation was not observed with rickettsia rickettsii.1977403144
[rickettsial arteritis due to coxiella burnetii].case of an 48 year old man who has presented from 1968 to 1973 a lot of diseases such as: --mitral incompletence discovered in 1968 in madagascar island in spite of many previous clinical examinations; --acute pneumonia and heart failure in january 1973. serological reactions of ricketsia were quite positive; --acute thrombosis of right humeral artery in may 1973. it has been treated by surgical way, bay "desobstruction" and by pass and medical treatment chloramphenicol). pathologic endartery ha ...1977403895
ultrastructural investigations on surface structures involved in coxiella burnetii phase variation.by using the cytochemical staining procedure with concanavalin a, horseradish peroxidase, and diaminobenzidine, no surface carbohydrates with terminal alpha-glucosyl or sterically closely related residues could be detected on the cell walls of coxiella burnetii phases i and ii. using a polycationized ferritin derivative as a cytochemical probe, anionic binding sites were visualized in the electron microscope on cell membranes of c. burnetii phase ii, but not on phase i organisms. the sites appea ...1977404251
[seroepidemiological investigations on the prevalence of rickettsial antibodies in man in the federal republic of germany (author's transl)].1,600 random serum samples from inhabitants of hesse were investigated for rickettsial antibodies by complement fixation test. antibodies were found in 19 samples (1,2%) against rmsf-group antigen with titers between 1:10 and 1:640. these results confirm recent reports on natural foci of such rickettsial infections in the federal republic of germany. antibodies against rickettsia of the typhus-group or coxiella burnetii could not be detected by the complement fixation reaction. none of the sera ...1977410727
q fever in south kanara district: natural occurrence of coxiella burnetii in the tick (aponomma gervaisi)--preliminary report. 1979429027
persistence of coxiella burnetii in beetles. dermestes maculatus (dermestidae).coxiella burnetti persisted irregularly in the larvae, pupae and imagos of dermestes maculatus till 8th week after oral infection. coxiella was probably transmitted between individual developmental stages by passages in the digestive tract.1979447127
the occurence of coxiellosis among rodents and shrews in the tarai area of uttar praedesh.rodents and shrews were screened for serologic evidence of coxiella burnetii. attempts were made to isolate the organism from the spleen and liver. seroreactors: total positive/total tested (% positive), in rats (rattus rattus, r. norvegicus), ground shrews (suncus murinus), bandicoots (bandicota indica, b. bengalensis) and the house mouse (mus musculus), respectively, were 13/105 (12.38), 6/42 (14.3), 2/15 (13.3) and 1/7 (14.3). of the eight rickettsial isolants recovered including four from fi ...1979459035
poikilotherms as reservoirs of q-fever (coxiella burnetii) in uttar pradesh.water snakes (natrix natrix), rat snakes (ptyas korros), cobras (naja naja), pythons (python molurus), tortoises (kachuga sp.), plankton fish (cirrhina mrigala), frogs (rana tigrina), toads (bufo sp.) and monitors (varanus indicus) were screened for evidence of q-fever infection by the capillary agglutination test on sera to detect antibodies and/or by attempts to demonstrate coxiella burnetii in spleen and liver samples. sero-reactors were observed among water and rat snakes, pythons and tortoi ...1979459042
[experimental exacerbation of latent q rickettsial infection].the evidence of the prolonged persistence of coxiella burnetii in experimental animals was obtained. cyclophosphamide was shown to be capable of activating q rickettsiosis in guinea pigs, and the period of the aggravation of this infection was found to be accompanied by a decrease in igg concentration and a relative increase in igm content.1979474001
q fever endocarditis in the united states.a patient with q fever endocarditis, which is almost unknown in the united states, was followed for a total of 32 months; the study was begun 3 1/2 months before aortic valve replacement. diagnosis was confirmed by serology, visualization of coxiella burnetii in excised aortic valve tissue by direct and immunofluorescence staining, and isolation of c. burnetii from aortic valve tissue. serum antibodies against phase i and phase ii antigens of c. burnetii were identified. almost all phase i and p ...1979475167
[a method for production of coxiella burnetii antigen and cell walls by guanidiniumchloride extraction (author's transl)].by use of 6-molar guanidinium chloride a potent coxiella burnetii antigen could be produced for diagnostic purposes from infected yolk sacs, with little technical expense. this treatment did not only a cause remarkably purifying effect (tab. 1) but also the extraction of soluble cytoplasmic substance. from guanidinium chloride-treated suspensions a highly purified and uniform suspension of cell walls could be separated by saccharose density gradient centrifugation (fig. 2). guanidine extracted o ...1979506552
[combined use of serological tests for the purpose of the diagnosis and study of q-rickettsiosis].the combined use of the complement fixation test, the indirect immunofluorescent test and the ring precipitation test with c. burnetii antigen greatly enhances the effectiveness or serological study, as it allows not only to find out the spread of infection among population more completely, but also to differentiate, to a certain extent, "fresh" infectious process from immunological trace reaction. the arguments are presented in favor of introducing the antigen of c. burnetii, phase i, into prac ...1979516960
survey of q-fever agglutinins in birds and small rodents in northern california, 1975-76.serum samples from 15 species of rodents and 33 species of birds were tested for agglutinins against coxiella burnetii by the microagglutination test. of 759 rodents tested, 21 (3%) were seropositive. antibody positive rodents included muskrats, ondatra zebethica, (11%), rattus spp. (10%), beechey ground squirrels, otospermophilus beecheyi, (6%), wood rats, neotoma fuscipes, (5%), and peromyscus spp. (2%). of 583 birds tested, 118 (20%) were seropositive. this included white crowned sparrows, zo ...1979522220
an occurrance of q fever in yuma county. a sporadic case of coxiella burnetti. 1979526146
sero-epidemiological studies on coxiellosis in animals and man in the state of uttar pradesh and delhi (india).the prevalence of antibodies to coxiella burnetii, the causative agent of q-fever, was studied in domestic animals either at the farm or at the slaughter house and man in delhi and uttar pradesh (u.p.). evidence of q-fever infection was observed in 24.29% of 490 cattle, 16.79% of 536 sheep, 16.02% of 1011 buffaloes, 15.85% of 1937 goats, 14.67% of 184 pigs and 14.29% of 49 street dogs. twenty percent of 55 dairy cows, 9.52% of 21 dairy goats and 5.71% of 35 dairy buffaloes were found positive fo ...1979536122
complement fixing and agglutinating antibodies to coxiella burnetii in several mammals of karnataka state. 1979541016
coxiellosis in reptiles of south kanara district, karnataka. 1979541017
[endocarditis by coxiella burnetti. a chronic form of q fever. report of one case (author's transl)].the authors describe a case of chronic endocarditis by q fever, in a patient who had been operated for coarctation of the aorta twelve years previously and at the same time was carrier of a congenital bivalve aorta. the clinical picture was suggestive of subacute endocarditis, but the blood culture was negative repeatedly. there was also a prolonged and relapsing febrile syndrome over a period of one-year-and-a-half. the following data are also worthy of note: the coexistence of a liver disorder ...1979547128
investigation of coxiella burneti infection as a possible cause of chronic liver disease in man.the possible role of coxiella burneti as a cause of chronic liver disease in man was investigated in cyprus. serology, using the complement fixation test and phase 1 and phase 2 antigens, was performed on 16 patients with cryptogenic cirrhosis and two patients with chronic active hepatitis. antibody studies were also done on 106 adult cypriot villagers and on 13 shepherds from flocks infected with c. burneti, to provide a base line for comparative purposes. no evidence was found to implicate the ...1979555068
q fever and animal abortion in cyprus.seventy-eight british soldiers stationed in the eastern sovereign base area (esba) in cyprus contracted q fever in the period december 1974 to june 1975. pneumonia developed in 59% of cases. of 31 patients tested, 81% had biochemical evidence of hepatitis although only one became clinically jaundiced. three patients (4%) suffered pericarditis. treatment with tetracycline had no apparent effect on the course of the disease. investigation revealed an abortion epidemic involving 21 mixed flocks of ...1977558666
role of brucella and coxiella burnetii infections in pyrexia of unknown origin. 1977566726
prevalence of coxiella burnetii infection among humans and domestic animals of rajasthan state, india.a total number of 1806 sera comprising 1049 humans and 757 animals from four ecologically different areas of rajasthan state were tested to determine the prevalence of complement-fixing (cf) antibodies to coxiella burnetii (c burnett). of the 1049 human and 757 animal sera tested, antibodies to c. burnetii were detected in 195 sera (18.6 per cent) and 187 sera (24.7 per cent), respectively. among humans, the prevalence of infection with c. burnetii was highest in the desert area of barmer distri ...1979574885
[rickettsial diseases (author's transl)]. 1979581906
a seriological investigation of q fever in clinical patients.results of a serological survey of 507 patients, 301 males and 206 females at the ahmadu bello university teaching hospital indicated a total 20.5 per cent, or 21.3 per cent in males and 19.4 per cent females, had been exposed to q fever agent and had circulating antibody against coxiella burnetii. most of the positive sera were from patients whose clinical diagnoses were typhoid and pyrexia of unknown origin though these generally are the commonest clinical diagnoses in the hospital. titres var ...1977592464
[biological properties of burnet's rickettsia isolated from different species of ixodes ticks]. 1977604760
experimental q-fever infection in guineapig. 1977611100
q fever antibodies in food animals of nigeria : a serological survey of cattle, sheep, and goats. 1977615325
changes in some popliteal lymph node and spleen lysosomal enzymes following the infection or vaccination of guinea-pigs with coxiella burneti. 1977615568
mesangio-capillary glomerulonephritis associated with q-fever endocarditis.a patient with nephrotic syndrome and q-fever endocarditis (confirmed serologically and ultrastructurally) was found to have mesangio-capillary glomerulonephritis with parietal deposits of c3 and igm and some igm in the mesangium. elution studies showed that igm antibodies reactive against insoluble coxiella antigens were present in the kidney. review of the literature suggests that this type of immune complex nephritis may be associated with q-fever. possible reasons for the variability of the ...1977615847
cell-mediated immune responses of guinea pigs to an inactivated phase i coxiella burnetii vaccine.the ability of a killed phase i coxiella burnetii vaccine to induce cell-mediated immune responses in guinea pigs was studied. cell-mediated immune responses were assessed by the inhibition of macrophage migration and lymphocyte transformation assays. the macrophage migration response occurred rapidly and was detected at high levels, but was relatively short-lived. in contrast, the lymphocyte transformation response developed more slowly, and persisted for a longer period. the vaccine, given in ...1978624586
limits of rickettsial infectivity.seven species of pathogenic rickettsiae were compared in five assay systems for group, species, strain, and phase differences in infectivity. the species examined include rickettsia prowazekii (breinl and cairo 3 strains), r. typhi, r. canada, r. rickettsii (sheila smith and r strains), r. conorii, r. sibirica, and coxiella burnetii in phases i and ii. pathogenicity was charcterized in terms of fever in guinea pigs. all comparisons of infectivity and pathogenicity were described in terms of numb ...1978624588
influence of etiology on the functional result of aortic valve replacement.the functional result of aortic valve replacement has been assessed in patients treated for isolated aortic incompetence. using maximal oxygen uptake as an index of myocardial function, a significant difference (p less than .05) exists between the patients who had q fever endocarditis on the one hand and those who had bacterial endocarditis or rheumatic fever on the other hand. we believe that permanent myocardial damage occurs as a result of bacterial endocarditis and rheumatic fever but becaus ...1978636982
effects of infection with coxiella burnetii on synthesis of rna in l cells.experimental infections with coxiella burnetii augment rates of ribonucleic acid (rna) synthesis in guinea pigs. the activity of deoxyribonucleic acid-dependent rna polymerase in l cells persistently infected with c. burnetii was threefold greater than that in unifected cells; the polymerase activity in infected cells was predominantly of class i, whereas that in uninfected cells was predominantly of class ii. a search for regulatory factors of polymerase activity revealed that preincubation of ...1978649991
evidence for the existence of q fever in northwest texas. 1978655321
serum q fever antibodies in swedish un soldiers in cyprus--reflecting a domestic or foreign disease?swedish soldiers from 2 battalions serving in cyprus 1975 and 1976 were tested serologically for coxiella burnetii phase 2 complement-fixing antibodies. 2.7% were positive. although none of the seropositive soldiers had been seriously ill abroad, the titers are considered to emanate from an infection in cyprus as there is no evidence of domestic q fever from a survey of 1390 swedes.1978675166
ultrastructural changes in guineapig liver experimentally infected with coxiella burnetii. 1978680848
seroprevalence of q fever infection in human beings from southern region of kerala state. 1978680877
experimental infection of coxiella burnetii in chicken: clinical symptoms, serologic response, and transmission through egg.young laying hens were infected with coxiella burnetii to study egg transmission, clinical and serologic responses, excretion of the agent in feces, and its persistence in internal organs. no clinical symptoms were noticed. the birds developed good titers in a capillary agglutination test by 13 days postinfection (dpi), which peaked at 30 dpi and therafter declined gradually, becoming negative in some birds around 90 dpi. in vivo and in vitro egg transmission of the agent could not be demonstrat ...1978697657
q fever antibodies in domestic animals in south kanara--a preliminary report. 1978700847
prevalence of coxiella burnetii (q fever) and toxoplasma gondii among dairy goats in california.the prevalence of antibodies against coxiella burnetii and toxoplasma gondii, the causative agents of q fever and toxoplasmosis, was examined in goats to determine the extent of the potential of goats to tansfer these infections to persons. of 1,054 goats from 234 premises in california, 248 goats (24%) were seropositive for c burnetii and 246 (23%) were seropositive for t gondii. of 29 dairy goats tested, 2 (7%) were found to be shedding the q fever rickettsia in their milk; t gondii was not de ...1978727592
experimental q fever infection in congenitally athymic nude mice.congenitally athymic nude (nu/nu) mice and their phenotypically normal (nu/+) euthymic littermates were exposed to coxiella burnetii administered as small-particle aerosols. after challenge, both strains of mice became infected, as characterized by rickettsemia, viable rickettsiae in the spleen, and serological conversion. the major difference noted was that euthymic animals had cleared rickettsiae from peripheral circulation and the spleen within 14 days. in contrast, rickettsiae were detected ...1978730352
enhancement of the immunogenicity of phase i antigen of coxiella burnetii.the immunogenicity of the soluble phase i antigen of coxiella burnetii for guinea pigs was enhanced by a nuclease-resistant complex of polyriboinosinic-polyribocytidylic acid, poly-l-lysine, and carboxymethyl cellulose.1978730378
mitogenic and protective activity associated with a lipopolysaccharide from coxiella burnetii.a purified lipopolysaccharide (lps) isolated from the rickettsial agent of q fever, coxiella burnetii phase 1, possesses some of the in vivo and in vitro biological properties previously only associated with bacterial endotoxins. the coxiella lps is mitogenic for guinea pig leukocytes, induces non-specific resistance in mice to virulent candida albicans, causes dermal schwartzman reactions, and is positive by the limulus lysate assay.1978747820
abortion associated with coxiella burnetii infection in dairy goats. 1978748297
sero-epidemiological evidence of coxiella burnetii infection among selected human population in calcutta. 1978750441
experimental coxiella burnetii infection of guinea pigs and mice.the susceptibility of five inbred strains of mice, designated dba/1j, dba/2j, c57bl/6j, balf/cj, and akr/j, as well as outbred hartley and moen-chase guinea pigs to infection with coxiella burnetii by several routes was studied. the dba/2j mice were more susceptible to infection and had higher mortality rates than other strains of mice. guinea pigs were more susceptible to infection than mice. lesions observed in the infected animals were similar to those previously described in man and experime ...1978750726
histopathological changes during experimental infection with coxiella burnetii in guinea-pigs (correlation with histochemical changes of rna). 1978751596
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