| chlorpyrifos and 3,5,6-trichloro-2-pyridinol: residues in the body tissues of cattle wearing chlorpyrifos-impregnated plastic ear bands. | | 1978 | 84823 |
| [role of anti-brain antibodies in the development of mineralocorticoid deficiency]. | | 1979 | 85292 |
| [relationship of the structure of specific somatic antigen polysaccharides in shigella dysenteriae bacteriae to the immunochemical properties]. | | 1979 | 85516 |
| preganglionic neurons in the sacral spinal cord of the rat: an hrp study. | | 1979 | 86176 |
| effects of antiprostatic agents on 5alpha-dihydrotestosterone binding to rat hypophyseal and hypothalamic cytosol macromolecules. | the binding of 5alpha-dihydrotestosterone to the hypophyseal and hypothalamic cytosol macromolecules prepared from castrated male rats was observed. the effects of antiprostatic agents on 5alpha-dihydrotestosterone binding to both hypophyseal and hypothalamic cytosol macromolecules was examined. cyproterone acetate and chlormadionone acetate showed the significant inhibiting effects on 5alpha-dihydrotestosterone binding to 7-8 s macromolecules of cytosol from both hypophysis and hypothalamus. sc ... | 1979 | 86441 |
| potentiating effect of nitrate and other anions on contraction in vascular smooth muscle. | | 1978 | 87173 |
| immunologic studies of native and modified human factor viii/von willebrand factor. | factor viii/von willebrand factor (fviii/vwf) is a glycoprotein with a molecular weight greater than one-million daltons. two activities are associated with this large molecule: fviii procoagulant activity and vwf activity. incubation of fviii/vwf with proteolytic enzymes causes rapid inactivation of the fviii procoagulant activity but has little effect on the vwf activity or antigenicity. in an attempt to gain insight into the structural features required for these two activities, antisera were ... | 1979 | 88237 |
| separation of human leukocyte interferon components by concanavalin a-agarose affinity chromatography and their characterization. | human leukocyte interferon (hl-if), produced by mixed leukocytes infected with newcastle disease virus, was resolved into three distinct fractions when chromatographed on concanavalin a-agarose. the major portion (70--75%) of interferon appeared in the breakthrough (bt fraction). the bound interferon (25--30%) was displaced from the column as two peaks: the first was eluted with 0.01 m methyl alpha-d-mannoside, yielding 15-20% of the interferon activity (alpha-mm fraction), and the second by inc ... | 1979 | 92998 |
| deficiency of the ninth component of complement in man. | the studies of serum from a case with c9 (the ninth component of complement) deficiency are described. a 29-year-old woman in good health was found to have low serum complement levels (ch50). c9 of her serum was undetectable by the hemolytic assay and by the immunochemical analysis but all other components were normal. it was demonstrated that low ch50 of her serum was due to the hemolysis of the sensitized sheep erythrocytes (ea) by the complement components from c1 to c8. | 1979 | 95812 |
| [the adrenergic innervation of the thoracic lymphatic duct in dogs in postnatal ontogeny]. | using histochemical fluorescence technique, studies have been made on the adrenergic innervation of the wall of the thoracal lymphatic duct in postnatal development of puppies. adrenergic fibers were found in the adventitial layer of the duct. during postnatal development of the animals, adrenergic innervation of this duct becomes more perfect, reaching the level of adult animals to the age of 2-3 months. | 1979 | 95857 |
| naturally occurring diatomaceous pneumoconiosis in sub-human primates. | | 1978 | 96157 |
| the fetus, pregnancy and parturition. | | 1977 | 98095 |
| anti-actin antibodies of human and rabbit origin. | the production of actin antisera in rabbits was described and their reaction in various immunological tests were compared with the results obtained with human anti-actin sera (smooth muscle antibodies). even if the sera may react with different antigenic determinants of actin as suggested by results in immunoprecipitation and blocking experiments, the rabbit and human sera can be used for studies of cells with equal or similar results. a prerequisite was that the rabbit anti-actin titres were hi ... | 1978 | 98096 |
| immunofluorescence and electron microscopic studies on kidney, choroid plexus and pituitary in rhesus monkeys immunized with the anti-hcg vaccine pr-beta-hcg-tt. | | 1978 | 98291 |
| aerosolized antigen dose-response studies in asthmatic monkeys. | this study used rhesus monkeys which had consistent respiratory responses to a standard (single dose) aerosolized concentration of a. beginning at lower concentrations of a which did not produce an airway response, fourfold increases in concentrations of a were aerosolized until an airway response occurred. compared with the single-dose sac after the tac, some consistently reactive animals did not respond to the sac, the maximal concentration of a used for testing. thus the tac may result in an ... | 1978 | 98604 |
| immunochemical analysis of the conformational properties of intermediates trapped in the folding and unfolding of bovine pancreatic trypsin inhibitor. | | 1978 | 98640 |
| differential tolerance to pentobarbital in rats bred for differences in alcohol sensitivity. | two lines of rats bred for differences in motor impairment following alcohol treatment were also found to be differentially affected by sodium pentobarbital in three experiments. the most affected (ma) animals, bred for sensitivity to alcohol, showed a decrement in stabilimeter activity at doses of 8 mg and 16 mg pentobarbital per kg body weight. the least affected (la) animals, bred for insensivity to alcohol, were affected only by the higher dose, at which the resulting impairment was still le ... | 1978 | 98785 |
| peptide hydrolases of lactobacillus casei: isolation and general properties of various peptidase activities. | discovery of an endopeptidase by gel chromatography and separation of 3 exopeptidases (a dipeptidase, an aminopeptidase and a specific carboxypeptidase) from lactobacillus casei ncdo 151 by affinity chromatography is described. the 3 exopeptidases were strongly inhibited by the metal chelators edta and 1,10-phenanthroline but were reactivated with co2+ and mn2+. the ph optima for aminopeptidase, dipeptidase and carboxypeptidase activities were 6.5, 7.6 and 7.2, respectively. maximum activity was ... | 1978 | 101561 |
| biochemical genetics of macaques. ii. glucosephosphate isomerase polymorphism in rhesus monkeys. | | 1978 | 103539 |
| congenital defects due to hyperthermia. | | 1978 | 104560 |
| penicillin-resistant gonorrhea. | | 1979 | 106997 |
| [sarcocystis and toxoplasma infections in slaughter sheep in bavaria]. | | 1979 | 107950 |
| persistence of ketosis despite suppression of lipolysis by 16,16-dimethylprostaglandin e2 in experimental diabetic ketoacidosis in rats. | | 1979 | 113273 |
| developmental patterns of peripheral nerve, myoneural junction and muscle: a review. | | 1977 | 148071 |
| radioimmunoassay for the detection and quantitation of 5-fluorodexoyuridine. | a sensitive radioimmunoassay for 5-fluorodeoxyuridine (fdurd) and 5-fluoro-2'-deoxyuridine 5'-monophosphate has been developed by using antibody induced in rabbits, [3h]fdurd, and a separation technique with nitrocellulose filters. antibody specificity was characterized by using compounds chemically related to fdurd and comparing their effectiveness for displacing [3h]fdurd from the antibody-combining site. drug levels in serum samples from patients receiving fdurd chemotherapy were easily and r ... | 1978 | 148966 |
| guinea-worm infection in banaskantha district of gujarat--some important epidemiological aspects. | | 1978 | 153328 |
| determination of estrogens in pregnancy urine by hemoagglutination inhibition reaction. | a simple and rapid hemoagglutination inhibition reaction system for the estimation of estrogens in pregnancy urine is described. results obtained by this method showed a good correlation with those measure by a conventional radioimmunoassay method and brown's colorimetric method. ninety-seven pregnancy urine samples from various gestational weeks were subjected to the assay, and the usefulness of this method as a screening test for high-risk pregnancy is discussed. | 1975 | 173188 |
| plotting the milky way. | | 1979 | 257577 |
| influenza virus split vaccines. effect of priming infection on serologic response in humans. | | 1979 | 262510 |
| influence of temperature on isometric contractions of rabbit atria during inotropic interventions. | inotropic effects on strips of left atria promoted by 1-epinephrine, increase in frequency, post-extrasystolic potentiation, and by increase in extracellular calcium concentration were studied at 28 c and 36 c. the results show that at lower temperature the resulting changes in temporal parameters (activation time, deactivation time and duration above 10% of maximum force) are greater than those at the higher temperature. at 36 c inotropic interventions have more prominent effects on the tension ... | 1979 | 263167 |
| [presence of the parasite bartonella bacilliformis in the blood and its recovery in thymectomized mice. strains: 049, 35, 12 and 15]. | | 1977 | 279054 |
| the purification of orotidine-5'-phosphate decarboxylase from yeast by affinity chromatography. | we have prepared an affinity column for the purification of orotidine-5'-phosphate decarboxylase from yeast. the column effects a 3200-fold purification from yeast homogenate in one pass; simple additional steps produce enzyme that has been purified 6700-fold and is not contaminated by any other protein that can be detected by sodium dodecyl sulfate-acrylamide gel electrophoresis. overall, 35% of the activity present in the yeast is recovered as pure enzyme. the resin for the column is synthesiz ... | 1979 | 374408 |
| effects of nitroglycerin on cardiac function and regional blood flow distribution in conscious dogs. | the effects of intravenous infusion of nitroglycerin (ntg), 8 and 32 microgram/kg.min for 7 min, and of sublingual ntg, 1.2 mg, were examined on direct and continuous measurements of systemic, coronary, and regional hemodynamics, left ventricular (lv) dimensions, pressures, and myocardial contractility in conscious dogs. ntg induced sustained reductions in lv dimensions and transient increases in heart rate and dp/dt, and decreases in mean arterial pressure. initially ntg increased cardiac outpu ... | 1978 | 415619 |
| kinetics of in vitro bovine lymphocyte immunostimulation with a brucella abortus antigen. | a brucella abortus-soluble antigen was investigated, using in vitro assay of lymphocyte immunostimulation, to determine which concentration of this antigen and which period of incubation of the lymphocyte cultures would induce maximum specific lymphocyte immunostimulation as an additional method for further study of b abortus infection in cattle. soluble antigen was prepared from autoclaved cells of b abortus strain 1119-3. peripheral blood lymphocytes were obtained from cattle infected with b a ... | 1978 | 415646 |
| the effect of cortisol on the response of the depolarized, calcium-free mouse uterus to calcium [proceedings]. | | 1979 | 497599 |
| [latex-chagestest-reactions of immunsera against bartonella bacilliformis, haemobartonella muris and eperythrozoon coccoides (author's transl)]. | sera from 10 patients positive for carrion's disease were analysed by means of the latex chagas test. sera from 40 spf-rats experimentally infected with haemobartonella muris and 5 spf-mice experimentally infected with eperythrozoon coccoides were similarly tested. both the human and mice sera were negative whereas positive reaction was observed with rat sera. the nature of this positive reaction has not been clarified. | 1977 | 888185 |
| maintenance of spermatogenesis with testosterone or dihydrotestosterone in hypophysectomized rats. | | 1975 | 1151927 |
| phylogenetic relationships among the agent of bacillary angiomatosis, bartonella bacilliformis, and other alpha-proteobacteria. | bacillary angiomatosis (ba) and chronic bartonellosis are bacterial infections of humans which result in an unusual vascular proliferative tissue response. in order to determine their phylogenetic relationships, we have determined greater than 95% of the 16s rrna sequences for these two organisms by amplification directly from infected ba tissue and from a bartonella bacilliformis lyophilized culture. the ba agent and b. bacilliformis are closely related alpha-proteobacteria (98.5%), although th ... | 1992 | 1378524 |
| adhesion to and invasion of cultured human cells by bartonella bacilliformis. | bartonella bacilliformis was tested for its ability to adhere to and invade tissue culture cell monolayers. the parasite was able to efficiently bind and penetrate human dermal fibroblasts, human laryngeal epithelium, and human umbilical vein endothelial cells. exposure of the organism to immune serum prepared against a crude bartonella extract containing cell wall and membranous material resulted in decreased ability of the parasite to invade host cells. there was also an overall reduction in t ... | 1992 | 1398917 |
| a phage in bartonella bacilliformis. | bacteriophage-like particles were found in bartonella bacilliformis culture. the particles consisted of head (icosahedral), 40 nm in diameter, and tail, 16 nm in length. | 1992 | 1406375 |
| detection of bartonella bacilliformis in cultures, blood, and formalin preserved skin biopsies by use of the polymerase chain reaction. | a polymerase chain reaction (pcr) is described for the detection of bartonella bacilliformis, the etiologic agent of bartonellosis, which cannot be identified biochemically. amplification of a genomic 231 bp bartonella dna sequence permitted specific identification of 12 bartonella isolates from peruvian bartonellosis patients as well as detection of bartonella dna in blood samples and formaldehyde preserved skin biopsies. specificity of amplification products was confirmed by restriction fragme ... | 1992 | 1470840 |
| rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis. | nine strains of rochalimaea spp. that were isolated from patients over a period of 4.5 years were characterized for their enzyme activities, cellular fatty acid compositions, and dna interrelatedness among rochalimaea spp., bartonella bacilliformis, and afipia felis (cat scratch disease bacillus). all except one isolate, which was rochalimaea quintana, were determined to belong to a newly proposed species, rochalimaea henselae sp. nov. after recovery from clinical material, colonies required 5 t ... | 1992 | 1537892 |
| deformation factor: an extracellular protein synthesized by bartonella bacilliformis that deforms erythrocyte membranes. | bartonella bacilliformis, a hemotropic bacterium and the causative agent of the human disease bartonellosis, when incubated in a tryptone-based medium produces an extracellular factor, termed deformation factor (df), which induces extensive indentations and trenches in trypsinized erythrocyte membranes. the factor is stable during storage at 4 degrees c. it can be inactivated by proteases or brief heating to 70 to 80 degrees c, can be precipitated by ammonium sulfate, is nondialyzable, and is re ... | 1992 | 1541567 |
| interactions between live bartonella bacilliformis and endothelial cells. | bartonella bacilliformis, a gram-negative, flagellated, motile bacterium, is the etiologic agent of verruca peruana. it is found within the verruca, where it can form large cytoplasmic (rocha-lima) inclusions in endothelial cells. previously, an activity has been described in homogenates of b. bacilliformis that in vitro increases the proliferation of endothelial cells and their production of tissue-type plasminogen activator (t-pa) and in vivo is angiogenic. the aim of the present study was to ... | 1992 | 1583335 |
| murine antibody responses distinguish rochalimaea henselae from rochalimaea quintana. | rochalimaea henselae causes persistent bacteremia, bacillary angiomatosis, and parenchymal bacillary peliosis. detection of a specific antibody response to r. henselae infection may represent an alternative to cultivation as a means of diagnosis. we assessed the specificity of induced murine antibodies for antigens from r. henselae and the closely related species r. quintana. groups of cd-1 mice were inoculated with whole organisms of six strains of r. henselae and two of r. quintana. pre- and p ... | 1992 | 1629326 |
| bartonella bacilliformis stimulates endothelial cells in vitro and is angiogenic in vivo. | bartonellosis, a biphasic disease caused by motile intracellular bacteria, produces in its tissue phase a characteristic dermal eruption (verruga peruana) resulting from a pronounced endothelial cell proliferation. bacteria are found in the interstitium and within the cytoplasm of endothelial cells (rocha-lima inclusion). the aim of this study was to determine if bartonella bacilliformis produce a substance(s) that might be responsible for the vascular proliferation seen in the verruga. this was ... | 1990 | 1693472 |
| effects of doxorubicin on maturation of human monocytes in adherent and non-adherent cultures. | purified human monocytes were cultured for 2 h, 88 h, and 10 days in plastic tubes (adherent) and for 10 days in teflon foil bags (non-adherent). monocytes were incubated with doxorubicin by two short-term exposures (750 or 1500 ng/ml) for 1 h or by continuous exposure (75 ng/ml). maturation was monitored by measuring the intracellular activity of three metabolic enzymes and two acid hydrolases. expression of receptors for the fc moiety of immunoglobulin g (fcri, fcrii, fcriii), cd14, and hla-dr ... | 1990 | 1698073 |
| bb65, a major immunoreactive protein of bartonella bacilliformis. | a 65 kda protein (bb65) has been identified as one of the major specific antigens of bartonella bacilliformis, the causative agent of bartonellosis which is a bacterial infectious disease of inhabitants of the andes. the gene encoding this antigen (7b2) was isolated from an expression library made directly from randomly generated fragments of b. bacilliformis genomic dna using bartonella antibodies raised in rabbits and sera of bartonellosis patients. the bartonella 7b2 gene was expressed in esc ... | 1990 | 1700634 |
| molecular characterization and proposal of a neotype strain for bartonella bacilliformis. | bartonella bacilliformis, the etiologic agent of bartonellosis, was characterized biochemically and by dna hybridization, guanine-plus-cytosine content, genome size, and 16s rrna sequencing. dnas from the two strains in our collection exhibited 97% relatedness in hydroxyapatite reactions done at 55 degrees c (optimal reassociation criterion) and 100% relatedness in reactions done at 70 degrees c (stringent reassociation criterion). there was no evidence of divergence within the related sequences ... | 1991 | 1715879 |
| 16s rrna sequences of bartonella bacilliformis and cat scratch disease bacillus reveal phylogenetic relationships with the alpha-2 subgroup of the class proteobacteria. | the primary structures of 16s rrnas of bartonella bacilliformis, an isolate of the cat scratch disease (csd) bacillus, and a strain phenotypically similar to the csd bacillus were determined by reverse transcriptase sequencing. these microorganisms were found to be members of the alpha-2 subgroup of the class proteobacteria. the sequence from b. bacilliformis was most closely related to the rrna of rochalimaea quintana (91.7% homology), the etiologic agent of trench fever. the sequence from the ... | 1991 | 1719021 |
| taxonomic considerations of bartonella bacilliformis based on phylogenetic and phenotypic characteristics. | the 16s-rrna gene of bartonella bacilliformis was amplified using the polymerase-chain reaction (pcr). the amplification product was sequenced using a linear-pcr procedure and compared with other published 16s-rrna sequences. the results of this analysis placed b. bacilliformis in the alpha subgroup of the proteobacteria, and more specifically demonstrated its close phylogenetic relationship to rochalimaea quintana. this relationship is supported by similarities in the size and mean base composi ... | 1991 | 1778432 |
| [recurrent aseptic meningitis in periodic disease or mollaret's meningitis?]. | a 33 year-old sephardic jewish man with familial mediterranean fever (fmf), presented during a 7 year period, 6 episodes of aseptic meningitis, improving within less than 24 h after spinal tap. cerebrospinal fluid analysis showed a mixed leucocytic pleocytosis ranging from 100 to 1,000 cell/mm3. spinal fluid cultures for bacteria, viruses and viral antibodies were always negative. our case supports other reports showing that recurrent aseptic meningitis, although rare, may occur in fmf. it usual ... | 1991 | 1853039 |
| clinical, histologic, microbiologic, and biochemical characterization of the causative agent of bacillary (epithelioid) angiomatosis: a rickettsial illness with features of bartonellosis. | it has been suggested that bacillary (epithelioid) angiomatosis (bea) is a manifestation of cat scratch disease (csd). because of clinical similarity between this condition and the verruga peruana phase of bartonellosis, we sought to further characterize this disease as well as its causative agent and to compare it to bartonellosis. we isolated a small flagellated pleomorphic bacillus from skin lesions of two patients with bea. organisms were stained successfully with warthin-starry silver stain ... | 1991 | 1919046 |
| bacillary angiomatosis: a systemic opportunistic infection with prominent cutaneous manifestations. | bacillary angiomatosis is an opportunistic infection with systemic manifestations. although most cases have occurred in human immunodeficiency virus-positive patients, other immunosuppressed patients, and even seemingly immunocompetent individuals, can become infected. in addition to the well-characterized cutaneous manifestations, visceral involvement can occur and may be the only locus of infection. lymphadenopathy, bone or soft-tissue masses, fever, and hepatosplenomegaly can be presenting si ... | 1991 | 1931568 |
| structure of the human centromere at metaphase. | until recently the centromere was thought to be a relatively homogeneous region of densely packed heterochromatin with a single differentiated domain--the kinetochore--at its surface, representing the point of attachment of the mitotic spindle. we now know that the centromere of higher eukaryotes is composed of several domains that have been identified using antibody probes. somewhere within the domains are located both the factor(s) that control the disjunction of sister chromatids and the mole ... | 1990 | 2193435 |
| [the cultivation of hybridoma cells in agarose granules]. | the results of cultivation of agarose gel immobilized hybridoma cells producing monoclonal antibodies to human alpha 2-interferon are presented. the immobilized cultured cells retained the higher viability for 10-28 days without changing the growth medium, in comparison with cultured cells in control suspension. the cells immobilized in agarose gel demonstrated a tendency to polyploidization as was revealed by the technique of dna cytofluorimetry with hoechst 33258. | 1990 | 2238110 |
| an epidemic of oroya fever in the peruvian andes. | between february and october 1987, a febrile illness killed 14 persons and seriously affected at least 14 others in shumpillan, a remote peruvian mountain village of 353 people. the illness was characterized by fever, headache, chills, and pallor. the fatality rate of untreated cases was 88%. the patients, 71% of whom were male, were 1-75 years of age. fatal illnesses progressed from lethargy to coma to death in 3-60 days. patients treated empirically with chloramphenicol survived. bartonella ba ... | 1990 | 2316791 |
| histology, immunohistochemistry, and ultrastructure of the verruga in carrion's disease. | twenty-six verruga peruana nodules were studied. the presence of factor viii-related antigen and ulex europaeus lectin binding, and the ultrastructural finding of rudimentary cell junctions and pinocytotic vesicles establish the endothelial character of the proliferating cells in the verruga nodules. whereas superficial lesions could show an angiomatoid pattern, deep-situated nodules tended to present a compact type of growth. electron-microscopic studies have shown that bartonella bacilliformis ... | 1986 | 2428261 |
| common surface epitope of bartonella bacilliformis and chlamydia psittaci. | a serosurvey revealed intense cross-reactivity between bartonella bacilliformis and chlamydia psittaci. one of the cross-reacting bartonella antigens was identified as lipopolysaccharide which reacted with bartonella as well as with chlamydia serum antibodies. a monoclonal bartonella antibody bound to bartonella lipopolysaccharide as well as to the surfaces of bartonella bacilliformis and chlamydia psittaci. it was thus demonstrated that chlamydia psittaci carries a surface epitope identical to ... | 1988 | 2461660 |
| characterization of cdna clones encoding two putative isoforms of the alpha 1 subunit of the dihydropyridine-sensitive voltage-dependent calcium channel isolated from rat brain and rat aorta. | cdna clones encoding rat brain and rat aorta isoforms of the alpha 1 subunit of the dihydropyridine-sensitive, voltage-dependent calcium channel were isolated and sequenced. these tissue-specific cdna clones share significant amino acid similarity with the rabbit skeletal muscle calcium channel alpha 1 subunit (75% and 66% amino acid identity for rat brain and rat aorta isoforms, respectively). northern analysis revealed transcript sizes of 6.5 and 8.6 kb in aorta and 8.6 kb in brain. | 1989 | 2546805 |
| a simple and versatile method for the preparation of vector-primers by adapter-end-primer ligation. | a group of efficient cdna cloning strategies employs vector-primers where cdna synthesis starts from the oligo(dt)-primer tail, which is conventionally attached to cloning vectors by use of terminal deoxynucleotidyl transferase. an alternative, efficient and more versatile method of vector-primer preparation is to directly ligate, by use of t4 dna ligase, a double-digested vector, e.g., ptz18r/pst i/bam hi, to a synthetic (bam hi)-adapter-end-primer, 5'-pgatcc-tn or 5'-pgatcc-site-specific seque ... | 1989 | 2561065 |
| characterization of an epsp-like potential recorded remotely from the round window. | the whole-nerve cochlear action potential (cap), to tone burst stimulation, was recorded before and after application of tetrodotoxin (ttx) to the intact round window (rw) membrane. ttx abolished the cap leaving a residual negative potential without altering the summating potential (sp) or the cochlear microphonic (cm). the residual potential retained its polarity when recorded from scala vestibuli. the peak latency, amplitude, and tuning properties of the residual potential showed features simi ... | 1989 | 2600307 |
| nicotine gum and tobacco cessation: a review of the literature. | | 1989 | 2691088 |
| sulfuryl transfer catalyzed by pyruvate kinase. | sulfoenolpyruvate, the analogue of phosphoenolpyruvate in which the phosphate ester has been replaced by a sulfate ester, has been synthesized in three chemical steps from ethyl bromopyruvate in 40% overall yield. this compound is a substrate for pyruvate kinase, producing pyruvate and adenosine 5'-sulfatopyrophosphate. the latter compound has been identified by nmr spectroscopy and by comparison with an authentic sample. sulfuryl transfer from sulfoenolpyruvate is 250-600-fold slower than phosp ... | 1989 | 2719922 |
| variceal hemorrhage as a consequence of spontaneous rupture of a pancreatic pseudocyst into the splenic vein. | we describe a case of a 68-yr-old man who died as a consequence of a massive bleed from esophageal varices. at autopsy, a pancreatic pseudocyst that communicated directly with the splenic vein was found. histology confirmed that material from the pseudocyst had embolized to the peripheral portal veins, occluding many of them. | 1989 | 2788993 |
| steroidogenesis in liposomal system containing adrenal microsomal cytochrome p-450 electron transfer components. | purified adrenal microsomal p-450c21 and/or p-45017 alpha,lyase were incorporated with purified nadph-cytochrome-p-450 reductase into liposome membranes composed of phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine at a molar ratio of 5:3:1. the rate dependences of reduction of liposomal p-450c21 in the fast phase as well as progesterone hydroxylation activities of p-450c21 and p-45017 alpha,lyase on the reductase concentration in the liposome membranes suggested that electron ... | 1989 | 2804111 |
| hepatocytic lipoprotein receptors and intracellular lipoprotein catabolism. | hepatocytes, as the major site of synthesis and terminal catabolism of plasma lipoproteins, exert the major regulatory influence on the concentration of atherogenic lipoproteins in blood plasma and may thereby influence the rate of atherogenesis. the ldl receptor on the microvillous sinusoidal surface of hepatocytes mediates the catabolism of remnants of triglyceride-rich lipoproteins and ldl. binding of vldl remnants to the receptor, mediated by apo e, is of very high affinity and presumably mu ... | 1988 | 2847970 |
| a proton magnetic resonance study of the metabolism of n-methylformamide in the rat. | | 1986 | 2877837 |
| a model for combined morphological and functional investigations on the isolated mediobasal rat hypothalamus. | we have developed a model for combined morphological and functional in vitro studies of the isolated mediobasal hypothalamus (mbh) by considering two prerequisites: (1) the tissue must be well preserved, free of morphological artefacts and functionally unimpaired until the end of the in vitro incubation, and (2) the tissue must be processed for morphology in optimal conditions. to test our model we have studied some aspects of the luteinizing hormone-releasing hormone (lhrh) system in 4-month-ol ... | 1987 | 2883598 |
| improved sexual function in hemodialysis patients on recombinant erythropoietin: a possible role for prolactin. | as it was reported that correction of anemia in long-term hemodialysis patients by recombinant human erythropoietin (r-huepo) is associated with improved sexual function, we conducted the present study to further delineate the mechanism(s) by which this is brought about. serum prolactin, testosterone, and parathyroid hormone (pth) levels were followed during 4 months of r-huepo therapy. within 4 months of treatment with r-huepo, hematocrit values rose from 23.7 +/- 1.2 to 35.7 +/- 0.2% and hemog ... | 1989 | 2914405 |
| potassium secretion by the cortical collecting tubule: effect of c1 gradients and ouabain. | in various epithelia k and cl transport are molecularly coupled and kcl cotransport is dependent on the na-k pump. the present study examines 1) the effect of a bath-to-lumen cl gradient on k secretion during active na transport and 2) the effect of basolateral ouabain on k secretion and na absorption in the presence and the absence of a bath-to-lumen cl gradient. under symmetrical conditions there was significant k secretion (jk = -24.0 +/- 3.9 pmol.mm-1.min-1) and cl secretion (jcl = -15.7 +/- ... | 1989 | 2916662 |
| demonstration of a ouabainlike plasma compound in hypertension prone and hypertension resistant rats. | material extracted and partially purified from plasma of the sabra hypertension prone rats was found to be capable of 1) inhibiting the binding of 3h-ouabain to rat brain synaptosomes, 2) inhibiting the activity of rat brain microsomal na, k activated adenosine triphosphatase, and 3) increasing the contractile force of rat heart muscle. the results demonstrate the presence of a ouabainlike compound in the plasma of these rats. the plasma concentration of this compound in sabra hypertension prone ... | 1985 | 2993163 |
| effects of the active constituents of catha edulis on the neuromuscular junction. | (-)-cathinone and d-norpseudoephedrine (dne) in the dose range 0.2-1.2 mg/ml produced a reduction in contractions of skeletal muscle, evoked by direct and indirect electrical stimulation and antagonised the facilitatory action of physostigmine on the neuromuscular junction; but failed to antagonise a partial blockade induced by d-tubocurarine (dtb) as occurs with norepinephrine or epinephrine. the local anaesthetic actions of (-)-cathinone and dne were found to be almost equivalent to that of li ... | 1987 | 3037411 |
| analysis and preparation of bartonella bacilliformis antigens. | twenty-four antigens of bartonella bacilliformis, a bacterium which causes bartonellosis in residents of high altitude valleys of the andes, were identified by immunoblot and immunoprecipitation using rabbit anti-bartonella sera as well as sera of patients. the antigens were designated according to their relative molecular mass which ranged from 16 to 160 kda. twelve antigens were detected by antibodies in sera of bartonellosis patients using immunoblot, of which six antigens were detected by im ... | 1988 | 3044156 |
| [a study on enzyme-linked immunosorbent assay (elisa) for detection of escherichia coli of heat-labile enterotoxin]. | | 1988 | 3073018 |
| estrogenic control of spermidine/spermine n1-acetyltransferase activity in rat uterus. | | 1988 | 3076330 |
| epidemiological aspects of acute viral hepatitis in portugal. | epidemiological aspects of 400 consecutive in-patients with acute viral hepatitis, aged one to 81 years, were studied: 180 patients were less than 15 years old (mean age 6.9 years) and 220 were older (mean age 33.8 years). serum hepatitis markers were detected by ria. hepatitis a (ha) was diagnosed in 188 patients (47.0%), ages ranging from one to 34 years (mean age 8.5 years), hepatitis b (hb) in 163 patients (40.8%), ages ranging from two to 72 years (mean age 32.7 years) and non-a, non-b hepa ... | 1986 | 3086236 |
| determination of the effect of acetylation of specific lysine residues in human growth hormone on its affinity for somatogenic receptors by an affinity selection technique. | a technique is described to study the effect of acetylation of individual lysine residues in peptide hormones on the affinity for their receptors, and is illustrated for the case of human growth hormone (hgh) binding to somatogenic receptors. the hgh was partially acetylated with high specific activity [3h]-acetic anhydride and the product ([3h]-ac-hgh) was incubated with solubilised affinity-purified somatogenic receptors (from male rat liver) in the presence and absence of excess unlabelled hg ... | 1988 | 3122756 |
| the action of epidermal growth factor on human amnion prostaglandin e2 output. | the mechanism of stimulatory action of epidermal growth factor on term human amnion prostaglandin e2 production was studied. monolayer cultures of amnion epithelial cells from spontaneous vaginal deliveries were preincubated for 24 h with serum-free media and treated with epidermal growth factor, calcium ionophore a23187 (4.5 microm), and arachidonate. cumulative prostaglandin e2 output was not stimulated by epidermal growth factor (less than or equal to 200 ng/ml) or a23187 alone or the two add ... | 1988 | 3139271 |
| the cpg dinucleotide and human genetic disease. | reports of single base-pair mutations within gene coding regions causing human genetic disease were collated. thirty-five per cent of mutations were found to have occurred within cpg dinucleotides. over 90% of these mutations were c----t or g----a transitions, which thus occur within coding regions at a frequency 42-fold higher than that predicted from random mutations. these findings are consistent with methylation-induced deamination of 5-methyl cytosine and suggest that methylation of dna wit ... | 1988 | 3338800 |
| effect of chronic naltrexone administration and its withdrawal on the regional activity of neurons that contain norepinephrine, dopamine and serotonin. | a method is described that permits the simultaneous quantitation of norepinephrine (ne), dopamine (da) serotonin (5-ht) and their respective major metabolites, 3-methoxy-4-hydroxy phenylglycol (mhpg), 3-methoxytyramine (3-mt), dihydroxyphenyl acetic acid (dopac) and 5-hydroxyindole acetic acid (5-hiaa) in discrete brain regions. the ratio of mhpg/ne, dopac/da and 5-hiaa/5-ht was used to assess the effects of the chronic administration of the narcotic antagonist, naltrexone, and its withdrawal on ... | 1988 | 3370455 |
| experimental ischemia induces cell mitosis in the adult rat retina. | experimental occlusion of the central retinal vessels in albino rats followed by reperfusion for 2 or 4 days resulted in a highly active proliferative response. mitotic figures were produced in retinal vascular cells, presumed müller cells, retinal pigment epithelial cells, and possibly other cell populations in the retina. a large number of mitoses were observed in 60-100% of 50 experimental eyes with 2, 4 or 6 hr of experimental ischemia and 2 or 4 days of reperfusion and in none of the 37 con ... | 1988 | 3417399 |
| effects of ouabain on cytosolic calcium in lymphocytes, platelets and adrenomedullary cells. | it has been suggested that a circulating inhibitor of na/k atpase can stimulate natriuresis and cause vasoconstriction in essential hypertension by stimulating transmembrane na/ca exchange to produce increased cytosolic concentrations of ionized calcium ([ca++]i) in renal tubular and arteriolar smooth muscle cells. if this inhibitor affected [ca++]i in all cell types, then clinical assays for its presence could be applied to easily accessible cells such as blood cells or platelets, and the inhib ... | 1987 | 3429864 |
| successful rescue of microsurgically produced homozygous uniparental mouse embryos via production of aggregation chimeras. | homozygous uniparental mouse embryos, produced by microsurgical removal of the male pronucleus from fertilized eggs and diploidization of the female pronucleus with cytochalasin, were surrounded with blastomeres from normal embryos to produce chimeric embryos. a few of these chimeras developed into viable adults, and one female has reproduced using her homozygous uniparental cells as a source of gametes. the production and use of such chimeras in breeding programs could greatly shorten the perio ... | 1986 | 3462710 |
| determination of "active" cytochrome p-450 from relaxation kinetics of product formation. | we estimate the "active" part of cytochrome p-450, which is involved in a special substrate transformation, by measuring the initial change of the production rate as a function of the relaxation transitions between two different steady states of the reaction cycle of cytochrome p-450 using the light-reversibility of the carbon monoxide inhibition. the kinetic data of such relaxations are interpreted within a model cycle, which reduces the reaction cycle to three steps. the estimation of the rate ... | 1987 | 3496215 |
| elisa using whole legionella pneumophila cell as antigen. comparison between monovalent and polyvalent antigens for the serodiagnosis of human legionellosis. | an indirect enzyme-linked immunosorbent assay (elisa) using the six serogroups of whole l. pneumophila bound to microtitre plate wells is described for the serodiagnosis of legionellosis. comparative studies using monovalent antigen indicated a high correlation between elisa and indirect immunofluorescence antibody (ifa) tests (r = 0.90, p less than 0.001). testing 196 human sera by elisa using both monovalent and polyvalent antigens have established the efficiency of the polyvalent antigen for ... | 1986 | 3534093 |
| rapid autonomic tone regulation of atrioventricular nodal conduction in man. | the changes in p-p intervals and atrioventricular nodal (avn) conduction during the valsalva maneuver were studied in 17 patients. in spite of a significant decrease in the sinus p-p interval during phase ii of the maneuver (733 +/- 143 to 520 +/- 86 msec, p less than 0.005) and prolongation during phase iv (884 +/- 171 msec, p less than 0.01), there was no change in the ah interval (control: 78 +/- 15: phase ii: 76 +/- 15: phase iv: 72 +/- 14 msec, n.s.). in six patients consecutive p-p interva ... | 1987 | 3565243 |
| overaccumulation of 3-hydroxy-3-methylglutaryl-coenzyme-a reductase in a compactin(ml-236b)-resistant mouse cell line with defects in the regulation of its activity. | using a stepwise selection procedure, we have developed a line of mouse fm3a cells which is resistant to compactin (ml-236b), a competitive inhibitor of 3-hydroxy-3-methyl-glutaryl-coenzyme-a (hmg-coa) reductase. this line, designated cr200, expressed a stable phenotype of a greater than 100-fold higher resistance to the inhibitor and a 160-fold higher hmg-coa reductase activity than did the parental fm3a cell line when grown in the presence of low-density lipoprotein (ldl). in contrast to its a ... | 1987 | 3569277 |
| radiometric-high-performance liquid chromatographic assay for nicotine and twelve of its metabolites. | a sensitive, reproducible radiometric-high-performance liquid chromatographic assay has been developed to measure concentrations of nicotine and twelve of its metabolites in biological fluids. following administration of nicotine ([2-14c]pyrrolidine) to rats, the assay was used in a pharmacokinetic investigation. | 1987 | 3667778 |
| a prospective critical evaluation of in vitro thyroid function tests. | a number of 2 325 serum samples from a population of in- and outpatients were collected during a six-month period in order to evaluate the usefulness of various thyroid function tests in the clinical laboratory routine. the samples were analysed with the following thyroid function tests: total triiodothyronine (t3) (tt3), total thyroxine (t4) (tt4), free t3 index (ft3i), free t4 index (ft4i) and thyrotropin (tsh). one to two years after the primary evaluation, a follow-up was performed and the f ... | 1986 | 3766208 |
| isolated granulomatous giant cell vasculitis of the pulmonary elastic arteries. | a 66-year-old man who developed an occluded left main pulmonary artery, clinically demonstrated by perfusion scan and pulmonary angiography, is described. explorative thoracotomy revealed a granulomatous giant cell arteritis after pathologic examination of the surgically removed left lung. detailed clinical and laboratory evaluation excluded a systemic disease. | 1986 | 3767614 |
| entry of bartonella bacilliformis into erythrocytes. | bartonella bacilliformis, which causes the human diseases oroya fever and verruga peruana, binds to human erythrocytes in vitro and produces substantial and long-lasting deformations in erythrocyte membranes, including cone-shaped depressions, trenches, and deep invaginations. the deforming force is probably provided by the polar flagella of these highly motile bacteria. deep invaginations containing bacteria are commonly seen, and membrane fusion at the necks of the invaginations leads to the f ... | 1986 | 3770946 |
| [restoration of immunologic responsiveness by psk in tumor-bearing animals]. | psk is a protein-bound polysaccharide prepared from cultured mycelium of the basidiomycete coriolus versicolor. effects of psk on the immunologic responsiveness in tumor-bearing animals were investigated using syngeneic or allogeneic tumors in mice (lewis lung carcinoma, b16 melanoma, meth a fibrosarcoma, adenocarcinoma 755, x5563 plasmacytoma, colon 26, mopc 31c myeloma, sarcoma 180 and ehrlich carcinoma), rats (bc47 bladder carcinoma, walker 256 sarcoma and ah7974 hepatoma), hamsters (ha-1t tu ... | 1986 | 3789758 |
| the structure of the cuticle and sheath of the infective juvenile of trichostrongylus colubriformis. | the infective third-stage juvenile of trichostrongylus colubriformis is surrounded by its own cuticle as well as the incompletely moulted cuticle of the second-stage juvenile, which is referred to as the sheath. the sheath comprises an outer epicuticle, an amorphous cortical zone, a fibrous basal zone and an inner electron-dense layer. the basal zone of the sheath consists of three layers of fibres; the fibres are parallel within each layer, but the fibre direction of the middle layer is at an a ... | 1986 | 3799009 |
| plasmodium falciparum malaria: band 3 as a possible receptor during invasion of human erythrocytes. | human erythrocyte band 3, a major membrane-spanning protein, was purified and incorporated into liposomes. these liposomes, at nanomolar concentrations of protein, inhibited invasion of human erythrocytes in vitro by the malaria parasite plasmodium falciparum. liposomes containing human band 3 were ten times more effective in inhibiting invasion than those with pig band 3 and six times more effective than liposomes containing human erythrocyte glycophorin. liposomes alone or liposomes containing ... | 1985 | 3880920 |
| antibodies to bartonella bacilliformis as determined by fluorescence antibody test, indirect haemagglutination and elisa. | two strains of bartonella bacilliformis were cultured on columbia agar supplemented with 5% defibrinated human blood. antigens prepared from these cultures were used for determination of antibodies by fluorescence antibody test (fat) indirect haemagglutination (iha) and an enzyme immunoassay (elisa). one hundred and eighty-seven human sera from b. bacilliformis-endemic areas of peru were tested of which 63.6% were reactive. elisa was the most sensitive test, followed by fat and iha. igm antibody ... | 1985 | 3911360 |
| pseudoephedrine-induced hypertensive emergency: treatment with labetalol. | the case of a young man in hypertensive emergency following the ingestion of 840 mg of pseudoephedrine is reported. the hypertension, which peaked at 200/160 mm hg and was associated with severe headache and diaphoresis, responded expeditiously to two initial intravenous doses of labetalol. no rebound was observed following the administration of an additional dose in the intensive care unit, after which an uneventful one-day hospitalization ensued. the toxicity of pseudoephedrine is briefly revi ... | 1986 | 3947442 |
| regulated biosynthesis and divergent metabolism of three forms of hepatic apolipoprotein b in the rat. | studies using rat livers perfused with recycled, serum-containing medium plus [3h]leucine revealed that secreted vldl contain three forms of apolipoprotein b (apob), b-48, b-95, and b-100, all synthesized by the liver. the b-48/(b-95 + b-100) [3h]leucine incorporation ratio ranged from 0.22 to 3.25 with livers of rats fed different diets, and the ratio was positively correlated with the triglyceride secretion rate in most of the livers. generally, as more triglyceride was secreted, a greater pro ... | 1985 | 3973514 |
| cardiovascular effects of drugs acting on the autonomous nervous system, in awake, normotensive and hypertensive rats. | a study has been carried out in awake normotensive (wky), spontaneously hypertensive (shr) and renal hypertensive (rhr) rats in order to compare the reactivity of the autonomous nervous system using different indwelling catheterizations. in all cases, a jugular catheter was implanted for i.v. injections. the arterial cannula was implanted in the femoral artery (group 1) or in the right carotid artery (group 2), where the catheter was routed over 20 mm towards the heart, reaching the aortic arch. ... | 1985 | 4015261 |
| cerebellocerebral projection from the fastigial nucleus onto the frontal cortex in the cat. | two frontal cortical areas hidden in sulci were found to be responsive to stimulation of the cerebellar fastigial nucleus under pentobarbital anesthesia: one is the ventral bank of the cruciate sulcus and the other is the area surrounding the fundus of the presylvian sulcus which corresponds to subregions of the frontal eye field. the fastigial projection onto these areas via the thalamic ventromedial (vm) nucleus was identified electrophysiologically and morphologically. | 1985 | 4063817 |