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a passive hemagglutination test for diagnosis of trench fever due to rochalimaea quintana.a passive hemagglutination test devised for diagnosis of trench fever was easily performed and highly sensitive and specific. tanned sheep erythrocytes were sensitized with soluble antigen from rochalimaea quintana. the test detected antibody in six of seven cases of primary infection and in four cases of late, relapsed trench fever. titers of antibody ranged from 1:20 to 1:640. although both igm and igg antibody to r. quintana were detected by passive hemagglutination, igg appeared to be the ma ...197663526
[anemias secondary to parasitosis]. 197898817
[presence of the parasite bartonella bacilliformis in the blood and its recovery in thymectomized mice. strains: 049, 35, 12 and 15]. 1977279054
enzyme immunoassay and radioimmunoprecipitation tests for the detection of antibodies to rochalimaea (rickettsia) quintana. 1977320604
enzyme immunoassay of antibody to rochalimaea quintana: diagnosis of trench fever and serologic cross-reactions among other rickettsiae.enzyme immunoassay (eia) tests were used to diagnose trench fever and to determine cross-reactions of rochalimaea quintana with other rickettsiae. the results were compared with those obtained by counterimmunoelectrophoresis (cie). all sera from cases of primary or relapsed forms of trench fever were positive both in eia, with serum antibody titers of 1:20-1:640, and in cie, giving one to three precipitin lines. sera from patients with other rickettsial infections were also tested for reactivity ...1978351072
[verruga peruana in ecuador. apropos of 3 cases].three cases of peruvian verruca, one autoctonous from the town of guayaquil (ecuador), are described by the authors. a comparative study with the peruvian cases is made.1977364206
taxonomic relationship of vole agent to rochalimaea quintana.we investigated the recent claim that the vole agent, a rickettsia-like microorganism isolated from wild voles by baker in 1946, is actually a strain of rochalimaea quintana, the etiological agent of trench fever. the two organisms were compared on the basis of percent guanine-plus-cytosine content, genome size, deoxyribonucleic acid-deoxyribonucleic acid hybridization, polypeptide composition, and serological relationships. although the two organisms do have identical or nearly identical deoxyr ...1979393636
[the course of trypanosoma cruzi infection in nonsplenectomized spe rats with and without haemobartonella muris infection (author's transl)].rats were infected with h. muris from the first to the second week of their life. four weeks later 20 of the infected rats were inoculated with 1.5 x 10(5) t. cruzi. control animals were given the same number of parasites. in both experimental groups no hemoflagellate could be seen in blood smears four weeks after infection. the comparison of the course of infection of t. cruzi in rats with and without h. muris was made using t- and wilcoxon-test for pair differences. rats with h. muris infectio ...1977414392
vole agent identified as a strain of the trench fever rickettsia, rochalimaea quintana.the vole agent described by baker in 1946 was studied as an example of a bacterium that has been mistakenly regarded a rickettsia. unlike rickettsiae, the vole agent killed chicken embryos with great irregularity, multipled primarily at the surface of avian or mammalian cells and not intracellularly, produced colonies rather than plaques on chicken embryo monolayers under agar, and developed small colonies after 4 to 7 days of cultivation on blood plates. it was most conveniently cultivated on m ...1978417026
hemotropic bacteria. 1979481540
intraerythrocytic parasites in rodent populations of connecticut: babesia and grahamella species.a total of 612 peromyscus leucopus, 11 microtus pennsylvanicus, 21 clethrionomys gapperi, and 4 tamias striatus was collected in connecticut and examined for babesia and grahamella during 1976 and 1977. babesia antibodies were detected in sera of 9 p. leucopus collected from 4 sites. babesia parasites were not detected in the blood smears of captured rodents. subsequent splenectomy and subinoculation of blood from these rodents into susceptible animals failed to induce disease and no babesia was ...1979512755
[an outbreak of "wet tail" in hamsters due to tyzzer's organism (author's transl)].in a colony of golden hamsters, many animals were found to show signs of "wet tail", and spontaneous deaths occurred during two months. with this outbreak of wet tail and death, etiologic and pathologic studies were made on 11 cases supplied from the colony. gross pathologic examination disclosed hemorrhagic enteritis in 10 and necrotic foci in the liver in 5 out of 10 cases examined. a number of bacilli morphologically suggestive of tyzzer's organisms were detected on smears of cut surfaces of ...1977560308
morphological and cell association characteristics of rochalimaea quintana: comparison of the vole and fuller strains.the vole and fuller strains of rochalimaea quintana were grown on monolayers of mouse l cells irradiated 7 days previously and examined by light microscopy and scanning and transmission electron microscopy. most of the bacteria of both strains were shown to adhere to the l cells but remained in an extracellular location. cell division was frequently seen among the extracellular bacteria. the few intracellular bacteria seemed to be within vacuoles and did not multiply. attachment to the eucaryoti ...1978681283
[protozoa of small mammals in the neusiedlersee region].during the years 1975 and 1976, 169 mammals (26 species) were investigated for their parasites. it was possible to recognise 33 different species of protozoans, belonging to the following genera: trypanosoma, giardia, hexamita, entamoeba, eimeria, isospora, toxoplasma frenkelia, hepatozoon, babesia, balantidium, pneumocytis, grahamella, eperythrozoon.--epidemiology and eventual pathogenic effects are briefly discussed.1978727554
effect of specific antibody and complement on the survival of rochalimaea quintana in vitro.no bactericidal effect was produced when rochalimaea quintana was exposed for 1 h to a combination of high-titered anti-r. quintana rabbit serum and guinea pig complement.1978730348
hematological, splenic and adrenal changes associated with natural and experimental infections of trypanosoma lemmi in the norwegian lemming, lemmus lemmus (l.).both natural and experimental trypanosoma lemmi infections in norwegian lemmings caused slight anemias and hypoglycemia. leucocyte counts remained the same. adrenal and splenic hypertrophy were associated with natural infections whereas only splenic hypertrophy was present in the experimental infection. grahamella sp. infections were also associated with adrenal and splenic hypertrophy in wild lemmings. it is suggested that blood parasites which are associated with anemia, hypoglycemia and adren ...1978744544
[latex-chagestest-reactions of immunsera against bartonella bacilliformis, haemobartonella muris and eperythrozoon coccoides (author's transl)].sera from 10 patients positive for carrion's disease were analysed by means of the latex chagas test. sera from 40 spf-rats experimentally infected with haemobartonella muris and 5 spf-mice experimentally infected with eperythrozoon coccoides were similarly tested. both the human and mice sera were negative whereas positive reaction was observed with rat sera. the nature of this positive reaction has not been clarified.1977888185
blood parasites of local pigs in ibadan. 1976936304
letter: eperythrozoon wenyoni, a possible cause of anaemia in british cattle. 1976936451
autoimmune factors associated with anaemia in acute haemobartonella and eperythrozoon infections of rodents.eperythrozoon coccoides and haemobartonella muris produced in mice and rats respectively, essentially the same disease, characterized by anaemia, splenomegaly and in severe cases, haemoglobinuria with death. in both infections anaemia was associated with phagocytosis of erythrocytes by monocytes of the spleen, and with the presence of cold-active haema-glutinin for trypsinized red cells (cah). an antigen similar to the serum antigen (sa) associated with acute malaria and babesiosis was also foun ...1976946746
letter: the occurrence of eperythrozoon ovis in queensland. 1976962744
[bartonellosis]. 19761046395
[immunobiology of tuberculosis].r. koch showed in 1882 in his so-called fundamental experiment that tuberculous guinea pigs exhibit a vigorous immunity against re-infection. in investigations in 1930--40 we showed that this immunity is not mediated by humoral antibodies. it is a cellular process, a local and general activation of the macrophage system (the system of mononuclear phagocytes). the tuberculous granuloma consists of activated macrophages and their descendants (epitheloid and giant cells), which inhibit the growth a ...19761085918
selected topics in laboratory animal medicine volume xxiv: the hamster. 19751099881
implication of phlebotomus sand flies as vectors of bartonellosis and leishmaniasis as early as 1764.a written account implicating phlebotomus sand flies as vectors of carrion's disease and cutaneous leishmaniasis in peru was published by cosme bueno in 1764. bueno's report precedes other publications implicating sand flies in the transmission of human pathogens by nearly a century and a half.19751101379
[blood parasites of small wild mammals in czechoslovakia].the author investigated 4,853 specimens of wild living small mammals (43 species: insectivora, chiroptera, lagomorpha, rodentia, carnivora) from czechoslovakia for blood parasites. trypanosomes were found in talpa europaea, sorex alpinus, crocidura suaveolens, myotis myotis, apodemus sylvaticus, apodemus flavicollis, mus musculus, rattus norvegicus, clethrionomys glareolus and microtus agrestis. babesia was detected in myotis emarginatus, myotis myotis, clethrionomys glareolus, microtus arvalis ...19751158292
an indirect hemagglutination test for the diagnosis of eperythrozoon suis infection in swine.an indirect hemagglutination test was developed to detect naturally occurring eperythrozoonosis in swine and to follow experimentally induced infections serologically. the antigen used was prepared from the plasma of acutely infected, splenectomized pigs. the test was specific and reliable in detecting swine latently infected with the disease.19751163869
studies on feline haemobartonellosis. iii. scanning electron microscopy of haemobartonella felis. 19751237746
[is there chromomycosis in spain?].the first case of autochthonous chromomycosis in spain is described by the author. the patient lived in the valencia province and the mycologic investigation was carried out by prof. dante borelli of venezuela.19751241702
dendrocytes in verruga peruana and bacillary angiomatosis.verruga peruana and bacillary angiomatosis are two cutaneous diseases characterized by angiomatous growths linked to the presence of rickettsia-like organisms. these lesions are currently considered to be endothelial hyperplasias and to share many features. by immunohistochemistry and computerized image analysis, we studied the presence of factor-xiiia-positive dendrocytes in these lesions and compared our data with similar research in capillary angiomas and normal skin. other cell lines were st ...19921348435
serological response to "rochalimaea henselae" antigen in suspected cat-scratch disease.there are no generally accepted diagnostic tests for cat-scratch disease (csd), the cause of which is unknown. during the development of an indirect fluorescence antibody (ifa) test for detection of antibodies to "rochalimaea henselae", sera from patients with csd were found to have high titres to r henselae antigens. further tests with this assay showed that 36 (88%) of 41 patients with suspected csd had serum titres of 64 or more to r henselae antigen, that there was a low prevalence (3%) of s ...19921351130
naturally occurring "rochalimaea henselae" infection in domestic cat. 19921354314
possible role of rochalimaea henselae in pathogenesis of aids encephalopathy. 19921357365
characterization of a novel rochalimaea species, r. henselae sp. nov., isolated from blood of a febrile, human immunodeficiency virus-positive patient.isolation of a rochalimaea-like organism from a febrile patient infected with human immunodeficiency virus was confirmed. analysis of 16s rrna gene sequences, together with polymerase chain reaction and restriction endonuclease length polymorphism analysis of a portion of the citrate synthase gene, demonstrated that the agent is closely related to members of the genus rochalimaea and that the isolate is genotypically identical to the presumptive etiologic agent of bacillary angiomatosis. however ...19921371515
phylogenetic relationships among the agent of bacillary angiomatosis, bartonella bacilliformis, and other alpha-proteobacteria.bacillary angiomatosis (ba) and chronic bartonellosis are bacterial infections of humans which result in an unusual vascular proliferative tissue response. in order to determine their phylogenetic relationships, we have determined greater than 95% of the 16s rrna sequences for these two organisms by amplification directly from infected ba tissue and from a bartonella bacilliformis lyophilized culture. the ba agent and b. bacilliformis are closely related alpha-proteobacteria (98.5%), although th ...19921378524
identification of the rochalimaea henselae 16s rrna sequence in the liver of a french patient with bacillary peliosis hepatis. 19921385550
nuclear planimetry and dna flow cytometry of verruga peruana.verruga peruana is a vascular hyperplasia that is sometimes histologically mistaken for a malignant neoplasm. an infiltrative pattern with nuclear pleomorphism and mitoses is prominent in some lesions. from a series of lesions of verruga peruana, we selected five atypical lesions, two of them closely resembling epithelioid angiosarcoma. we used histologic criteria as well as morphometry and flow cytometry to further characterize the atypical features of the lesions. histology and morphometry evi ...19921388574
adhesion to and invasion of cultured human cells by bartonella bacilliformis.bartonella bacilliformis was tested for its ability to adhere to and invade tissue culture cell monolayers. the parasite was able to efficiently bind and penetrate human dermal fibroblasts, human laryngeal epithelium, and human umbilical vein endothelial cells. exposure of the organism to immune serum prepared against a crude bartonella extract containing cell wall and membranous material resulted in decreased ability of the parasite to invade host cells. there was also an overall reduction in t ...19921398917
a phage in bartonella bacilliformis.bacteriophage-like particles were found in bartonella bacilliformis culture. the particles consisted of head (icosahedral), 40 nm in diameter, and tail, 16 nm in length.19921406375
isolation of rochalimaea species from cutaneous and osseous lesions of bacillary angiomatosis.bacillary angiomatosis is characterized by vascular lesions, which occur usually in patients infected with the human immunodeficiency virus (hiv). a newly described gram-negative organism, rochalimaea henselae, has been associated with cutaneous bacillary angiomatosis, but no organism has been isolated and cultivated directly from cutaneous tissue.19921435899
infections due to rochalimaea: the expanding clinical spectrum. 19921457660
the causative agent of bacillary angiomatosis. 19921459755
detection of bartonella bacilliformis in cultures, blood, and formalin preserved skin biopsies by use of the polymerase chain reaction.a polymerase chain reaction (pcr) is described for the detection of bartonella bacilliformis, the etiologic agent of bartonellosis, which cannot be identified biochemically. amplification of a genomic 231 bp bartonella dna sequence permitted specific identification of 12 bartonella isolates from peruvian bartonellosis patients as well as detection of bartonella dna in blood samples and formaldehyde preserved skin biopsies. specificity of amplification products was confirmed by restriction fragme ...19921470840
immunocytochemical identification of rochalimaea henselae in bacillary (epithelioid) angiomatosis, parenchymal bacillary peliosis, and persistent fever with bacteremia.we report the immunocytochemical identification of rochalimaea henselae, a newly recognized fastidious, gram-negative, warthin-starry-positive organism, as the common pathogen in bacillary angiomatosis (ba), bacillary peliosis (bp) of the liver and spleen, and persistent fever with bacteremia in immunocompromised patients. immunogenic proteins of the r. henselae strain isolated from the blood of a febrile immunocompromised patient with bp of the liver were used to produce primary immune serum in ...19921530106
rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis.nine strains of rochalimaea spp. that were isolated from patients over a period of 4.5 years were characterized for their enzyme activities, cellular fatty acid compositions, and dna interrelatedness among rochalimaea spp., bartonella bacilliformis, and afipia felis (cat scratch disease bacillus). all except one isolate, which was rochalimaea quintana, were determined to belong to a newly proposed species, rochalimaea henselae sp. nov. after recovery from clinical material, colonies required 5 t ...19921537892
deformation factor: an extracellular protein synthesized by bartonella bacilliformis that deforms erythrocyte membranes.bartonella bacilliformis, a hemotropic bacterium and the causative agent of the human disease bartonellosis, when incubated in a tryptone-based medium produces an extracellular factor, termed deformation factor (df), which induces extensive indentations and trenches in trypsinized erythrocyte membranes. the factor is stable during storage at 4 degrees c. it can be inactivated by proteases or brief heating to 70 to 80 degrees c, can be precipitated by ammonium sulfate, is nondialyzable, and is re ...19921541567
rochalimaea henselae causes bacillary angiomatosis and peliosis hepatis.recent studies have demonstrated that a newly described agent of persistent bacteremia, rochalimaea henselae, and the agent of bacillary angiomatosis are both closely related to rochalimaea quintana. bacillary peliosis hepatis seemed likely to have the same etiologic agent as bacillary angiomatosis. we sought these pathologic changes in patients from whom r henselae was cultivated.19921546924
relapsing illness due to rochalimaea henselae in immunocompetent hosts: implication for therapy and new epidemiological associations.two previously healthy, immunocompetent men had persistent rochalimaea henselae bacteremia with clinical relapses after courses of antibiotics to which the isolates were ultimately demonstrated susceptible in vitro. both had sustained tick bites prior to their illnesses, thus demonstrating an association not previously identified, although suspected. the first patient had relapsing fever, constitutional symptoms, and an episode of aseptic meningitis despite therapy with amoxicillin, then with do ...19921562660
bacillary angiomatosis. a "new" disease with a broadening clinicopathologic spectrum.bacillary angiomatosis (ba) is a reactive vasoproliferative lesion occurring almost exclusively in immunocompromised individuals in response to infection by a bacillus closely related to rochalimaea quintana. the commonest site of involvement is the skin, in the form of multiple erythematous nodules, but bacillary angiomatosis can also present in a wide variety of sites such as soft tissues, bone, lymph node, liver and spleen. some patients may present with persistent fever and bacteraemia. baci ...19921576430
interactions between live bartonella bacilliformis and endothelial cells.bartonella bacilliformis, a gram-negative, flagellated, motile bacterium, is the etiologic agent of verruca peruana. it is found within the verruca, where it can form large cytoplasmic (rocha-lima) inclusions in endothelial cells. previously, an activity has been described in homogenates of b. bacilliformis that in vitro increases the proliferation of endothelial cells and their production of tissue-type plasminogen activator (t-pa) and in vivo is angiogenic. the aim of the present study was to ...19921583335
[a 60-year-old patient with recurrent angiomatous lesions]. 19921598432
murine antibody responses distinguish rochalimaea henselae from rochalimaea quintana.rochalimaea henselae causes persistent bacteremia, bacillary angiomatosis, and parenchymal bacillary peliosis. detection of a specific antibody response to r. henselae infection may represent an alternative to cultivation as a means of diagnosis. we assessed the specificity of induced murine antibodies for antigens from r. henselae and the closely related species r. quintana. groups of cd-1 mice were inoculated with whole organisms of six strains of r. henselae and two of r. quintana. pre- and p ...19921629326
genetics of rickettsiae.classical genetic approaches useful with free-living bacteria are difficult to apply to the rickettsiae. although rickettsial mutants have been isolated over the years, the genetic basis of these mutants is unknown, limiting their usefulness. the application of molecular biological techniques to rickettsial studies has provided the opportunity to isolate and study specific genes. genes encoding metabolic enzymes from rickettsiae were cloned in escherichia coli and shown to retain their regulator ...19911679397
transformation and genomic restriction mapping of rochalimaea spp.transformation procedures using electroporation were established for rochalimaea quintana. several cosmid/plasmids possessing the rk2 or rsf1010 origin of replication were successfully inserted. plasmid retention and replication were verified by antibiotic resistance and southern blot analysis. the highest level of transformation was obtained at a voltage field strength of 12.5 kv/cm with a pulse time of 10 miliseconds. transformation efficiency was low (0.3%) with approximately 10(5) transforma ...19911687634
bartonella bacilliformis stimulates endothelial cells in vitro and is angiogenic in vivo.bartonellosis, a biphasic disease caused by motile intracellular bacteria, produces in its tissue phase a characteristic dermal eruption (verruga peruana) resulting from a pronounced endothelial cell proliferation. bacteria are found in the interstitium and within the cytoplasm of endothelial cells (rocha-lima inclusion). the aim of this study was to determine if bartonella bacilliformis produce a substance(s) that might be responsible for the vascular proliferation seen in the verruga. this was ...19901693472
bb65, a major immunoreactive protein of bartonella bacilliformis.a 65 kda protein (bb65) has been identified as one of the major specific antigens of bartonella bacilliformis, the causative agent of bartonellosis which is a bacterial infectious disease of inhabitants of the andes. the gene encoding this antigen (7b2) was isolated from an expression library made directly from randomly generated fragments of b. bacilliformis genomic dna using bartonella antibodies raised in rabbits and sera of bartonellosis patients. the bartonella 7b2 gene was expressed in esc ...19901700634
molecular characterization and proposal of a neotype strain for bartonella bacilliformis.bartonella bacilliformis, the etiologic agent of bartonellosis, was characterized biochemically and by dna hybridization, guanine-plus-cytosine content, genome size, and 16s rrna sequencing. dnas from the two strains in our collection exhibited 97% relatedness in hydroxyapatite reactions done at 55 degrees c (optimal reassociation criterion) and 100% relatedness in reactions done at 70 degrees c (stringent reassociation criterion). there was no evidence of divergence within the related sequences ...19911715879
16s rrna sequences of bartonella bacilliformis and cat scratch disease bacillus reveal phylogenetic relationships with the alpha-2 subgroup of the class proteobacteria.the primary structures of 16s rrnas of bartonella bacilliformis, an isolate of the cat scratch disease (csd) bacillus, and a strain phenotypically similar to the csd bacillus were determined by reverse transcriptase sequencing. these microorganisms were found to be members of the alpha-2 subgroup of the class proteobacteria. the sequence from b. bacilliformis was most closely related to the rrna of rochalimaea quintana (91.7% homology), the etiologic agent of trench fever. the sequence from the ...19911719021
dermatopathologic findings in patients infected with hiv.several inflammatory, infectious, and neoplastic conditions in hiv-infected patients are distinctive or require a biopsy for diagnosis. some differ subtly from similar conditions seen in noninfected patients. the exanthem of acute hiv infection cannot be diagnosed specifically on biopsy as its histologic appearance is similar to that of other viral exanthemata. a condition that closely resembles seborrheic dermatitis occurs in hiv-infected patients. plasma cells, necrotic keratinocytes, and leuk ...19921730173
daniel carrión and carrión's disease. 19911742572
taxonomic considerations of bartonella bacilliformis based on phylogenetic and phenotypic characteristics.the 16s-rrna gene of bartonella bacilliformis was amplified using the polymerase-chain reaction (pcr). the amplification product was sequenced using a linear-pcr procedure and compared with other published 16s-rrna sequences. the results of this analysis placed b. bacilliformis in the alpha subgroup of the proteobacteria, and more specifically demonstrated its close phylogenetic relationship to rochalimaea quintana. this relationship is supported by similarities in the size and mean base composi ...19911778432
splenomegaly and reticulocytosis caused by babesia microti infections in natural populations of the montane vole, microtus montanus.a survey for babesia microti in rodents was conducted at six sites within grand teton national park, wyoming. blood and spleen smears, hematocrits, and reticulocyte counts were made on all of the animals to evaluate parameters for the diagnosis of babesiosis. ticks were removed for identification. of 257 microtus montanus, 103 were infected with b. microti. in addition, five of 12 microtus pennsylvanicus and one of three arvicola richardsoni were parasitized by b. microti. peromyscus maniculatus ...19911818201
cat-scratch disease and bacillary angiomatosis. 19911895469
clinical, histologic, microbiologic, and biochemical characterization of the causative agent of bacillary (epithelioid) angiomatosis: a rickettsial illness with features of bartonellosis.it has been suggested that bacillary (epithelioid) angiomatosis (bea) is a manifestation of cat scratch disease (csd). because of clinical similarity between this condition and the verruga peruana phase of bartonellosis, we sought to further characterize this disease as well as its causative agent and to compare it to bartonellosis. we isolated a small flagellated pleomorphic bacillus from skin lesions of two patients with bea. organisms were stained successfully with warthin-starry silver stain ...19911919046
the causative agent of bacillary angiomatosis. 19911922261
bacillary angiomatosis: a systemic opportunistic infection with prominent cutaneous manifestations.bacillary angiomatosis is an opportunistic infection with systemic manifestations. although most cases have occurred in human immunodeficiency virus-positive patients, other immunosuppressed patients, and even seemingly immunocompetent individuals, can become infected. in addition to the well-characterized cutaneous manifestations, visceral involvement can occur and may be the only locus of infection. lymphadenopathy, bone or soft-tissue masses, fever, and hepatosplenomegaly can be presenting si ...19911931568
bartonellosis. an immunodepressive disease and the life of daniel alcides carrión.the history of human bartonellosis, a unique south american biphasic bacterial disease, is reviewed. the course of this disease and its natural history offer many puzzling problems. its geographic distribution, its absence of a natural reservoir, the development of transient immunosuppression during the acute hematic phase, and the subsequent development of vascular proliferations are highlighted. these vascular proliferations represent a unique example of tissue reactivity recalling the vascula ...19912008885
the agent of bacillary angiomatosis. an approach to the identification of uncultured pathogens.bacillary angiomatosis is an infectious disease causing proliferation of small blood vessels in the skin and visceral organs of patients with human immunodeficiency virus infection and other immunocompromised hosts. the agent is often visualized in tissue sections of lesions with warthin-starry staining, but the bacillus has not been successfully cultured or identified. this bacillus may also cause cat scratch disease.19902233945
a newly recognized fastidious gram-negative pathogen as a cause of fever and bacteremia.we identified a motile, curved, gram-negative bacillus as the cause of persistent fever and bacteremia in two patients with symptomatic human immunodeficiency virus infection. the same organism was subsequently recovered from a bone marrow-transplant recipient with septicemia and from two immunocompetent persons with week-long febrile illnesses. all the patients recovered after antimicrobial therapy.19902233947
an epidemic of oroya fever in the peruvian andes.between february and october 1987, a febrile illness killed 14 persons and seriously affected at least 14 others in shumpillan, a remote peruvian mountain village of 353 people. the illness was characterized by fever, headache, chills, and pallor. the fatality rate of untreated cases was 88%. the patients, 71% of whom were male, were 1-75 years of age. fatal illnesses progressed from lethargy to coma to death in 3-60 days. patients treated empirically with chloramphenicol survived. bartonella ba ...19902316791
blood parasites of mammals from papua new guinea.thin blood smears were collected from 126 mammals representing four genera of marsupials and six genera of murid rodents. a species of hepatozoon was discovered in the new guinea spiny bandicoot (echymipera kalubu), trypanosome infections were found in three genera of rodent hosts and the prevalence of a rickettsial parasite of the genus grahamella was recorded in rodents from the genera rattus and melomys. dried blood samples also were taken and screened serologically for antibodies to arenavir ...19902338736
transformation of rochalimaea quintana, a member of the family rickettsiaceae.rochalimaea quintana is the only member of the family rickettsiaceae that can be grown in vitro. because of its relationship to the other members of this family, techniques developed to transform r. quintana might be applicable to the obligate intracellular bacteria of the rickettsiaceae. these procedures are critical to understanding mechanisms of pathogenesis and the nature of obligate intracellular growth. a transformation procedure for r. quintana has been established by using electroporatio ...19902394681
histology, immunohistochemistry, and ultrastructure of the verruga in carrion's disease.twenty-six verruga peruana nodules were studied. the presence of factor viii-related antigen and ulex europaeus lectin binding, and the ultrastructural finding of rudimentary cell junctions and pinocytotic vesicles establish the endothelial character of the proliferating cells in the verruga nodules. whereas superficial lesions could show an angiomatoid pattern, deep-situated nodules tended to present a compact type of growth. electron-microscopic studies have shown that bartonella bacilliformis ...19862428261
common surface epitope of bartonella bacilliformis and chlamydia psittaci.a serosurvey revealed intense cross-reactivity between bartonella bacilliformis and chlamydia psittaci. one of the cross-reacting bartonella antigens was identified as lipopolysaccharide which reacted with bartonella as well as with chlamydia serum antibodies. a monoclonal bartonella antibody bound to bartonella lipopolysaccharide as well as to the surfaces of bartonella bacilliformis and chlamydia psittaci. it was thus demonstrated that chlamydia psittaci carries a surface epitope identical to ...19882461660
bartonellosis and kaposi sarcoma of aids. 19892564943
the line blot: an immunoassay for monoclonal and other antibodies. its application to the serotyping of gram-negative bacteria.a procedure is described for assaying antibodies based on the application of antigen to nitrocellulose as a line with an ink pen point. the method requires no expensive apparatus, is easy to perform, and requires less than 0.25 micrograms of antigen per assay. more than 45 antigens can be assayed simultaneously with a single antibody. antigens can be applied as purified proteins, extracts, or sodium dodecyl sulfate solubilized extracts. the application of the line blot assay for the detection of ...19892607166
trench fever in belfast, and the nature of the 'relapsing fevers' in the united kingdom in the nineteenth century.some evidence is assembled to suggest that trench fever, an infection with a strain of rochalimaea, if not quintana, then vinsonii, was present in belfast in the first half of the nineteenth century in endemic and epidemic form. it may have amounted at times to one half or more of 'fever'. this may account for the comparatively low mortality in some years from 'fever'. the phrase 'relapsing fever' in the nineteenth and twentieth century medical literature of the united kingdom should not be take ...19892672525
[observations on carrión's disease]. 19892675250
bacillary (epithelioid) angiomatosis and concurrent kaposi's sarcoma in acquired immunodeficiency syndrome.two patients with acquired immunodeficiency syndrome developed simultaneous kaposi's sarcoma and bacillary (epithelioid) angiomatosis. the distinguishing clinical and histologic features of these two vascular proliferations associated with human immunodeficiency virus disease are described. the lesions of bacillary (epithelioid) angiomatosis contained bacteria, while the lesions of kaposi's sarcoma did not. with erythromycin therapy, the lesions of bacillary (epithelioid) angiomatosis cleared, w ...19892817918
selected observations on rickettsiae and their host cells.the study of the interaction between rickettsiae and their host cells is in its infancy. members of the genera rickettsia, coxiella and rochalimaea show considerable diversity in host cell range (in vivo vs. in vitro), kind of association with host cell (pericellular, intracellular), mode of entry, interactions with various host cell membranes, intracellular localization (intraphagosomal, free in cytoplasm, intranuclear), adaptation to preferred microhabitat (e.g., optimal ph for enzymes), detai ...19862871738
cat scratch disease, bartonellosis, and kaposi sarcoma. 19882904546
daniel a. carrion: 1858-1885. 19882905699
analysis and preparation of bartonella bacilliformis antigens.twenty-four antigens of bartonella bacilliformis, a bacterium which causes bartonellosis in residents of high altitude valleys of the andes, were identified by immunoblot and immunoprecipitation using rabbit anti-bartonella sera as well as sera of patients. the antigens were designated according to their relative molecular mass which ranged from 16 to 160 kda. twelve antigens were detected by antibodies in sera of bartonellosis patients using immunoblot, of which six antigens were detected by im ...19883044156
sandfly control perspectives. 19863092718
[chronic bartonellosis of the verruga peruana type (carrion's disease)]. 19883239909
[the man behind the syndrome: daniel carrión. he died when he experimented on himself with the peruvian national disease]. 19873298897
seasonal and age distributions of babesia, hepatozoon, trypanosoma and grahamella species in clethrionomys glareolus and apodemus sylvaticus populations.four common blood parasites belonging to the genera babesia, hepatozoon, trypanosoma and grahamella were detected in sympatric populations of clethrionomys glareolus and apodemus sylvaticus. seasonal changes in the abundance of hosts and parasites were monitored in a longitudinal field survey for 18 months. all parasites in both hosts showed similar seasonal patterns with most transmission occurring in summer and autumn and ceasing in winter. amongst aged subsets of each host population, hepatoz ...19863537923
verruga peruana mimicking malignant neoplasms.we have shown that in some cases fully developed florid verruga peruana nodules, as well as late-resolving, deeply situated lesions, can histologically suggest a variety of tumors to experienced pathologists. the compact proliferation of endothelial cells characteristic of florid verruga lesions can give rise to two pseudoneoplastic histologic patterns. one consists of sheets or islands of cells arranged in an epithelioid or pseudoepithelioid pattern (cases 1 and 2) in which the following histol ...19873631453
entry of bartonella bacilliformis into erythrocytes.bartonella bacilliformis, which causes the human diseases oroya fever and verruga peruana, binds to human erythrocytes in vitro and produces substantial and long-lasting deformations in erythrocyte membranes, including cone-shaped depressions, trenches, and deep invaginations. the deforming force is probably provided by the polar flagella of these highly motile bacteria. deep invaginations containing bacteria are commonly seen, and membrane fusion at the necks of the invaginations leads to the f ...19863770946
tissue reaction in bartonellosis may suggest kaposi's sarcoma. 19853839371
hundredth anniversary of carrión's death. 19853911359
antibodies to bartonella bacilliformis as determined by fluorescence antibody test, indirect haemagglutination and elisa.two strains of bartonella bacilliformis were cultured on columbia agar supplemented with 5% defibrinated human blood. antigens prepared from these cultures were used for determination of antibodies by fluorescence antibody test (fat) indirect haemagglutination (iha) and an enzyme immunoassay (elisa). one hundred and eighty-seven human sera from b. bacilliformis-endemic areas of peru were tested of which 63.6% were reactive. elisa was the most sensitive test, followed by fat and iha. igm antibody ...19853911360
[tribute to the peruvian martyr of medicine, dr. daniel alcides carrión, on the centenary of his sacrifice, 1885-1985]. 19853914220
a common origin of rickettsiae and certain plant pathogens.on the basis of ribosomal rna sequence comparisons, the rickettsia rochalimaea quintana has been found to be a member of subgroup 2 of the alpha subdivision of the so-called purple bacteria, which is one of about ten major eubacterial divisions. within subgroup alpha-2, r. quintana is specifically related to the agrobacteria and rhizobacteria, organisms that also have close associations with eukaryotic cells. this genealogical grouping of the rickettsiae with certain plant pathogens and intracel ...19853931222
[on the behavior of serum proteins in chickens during aegyptianella pullorum infection]. 19714102357
[human immunoglobulins and their role in infectious pathology (review)]. 19714105768
effects of selected tissue antisera--spleen-bone marrow, brain, thyroid, pancreas, pituitary, thymus, etc.--in vivo or in vitro: a review covering 30 years researches at the university of texas medical branch. 19744139777
acute febrile anaemia associated with bartonella-like erythrocytic structures. 19664169257
lymphoma: intranuclear bacilliform structures in a patient with febrile anemia. 19684172058
[incertae sedis parasites in comparative pathology]. 19724196197
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