| binding studies with antibodies having phosphorylcholine specificity and fragments derived from their homologous streptococcus pneumoniae type 27 capsular polysaccharide. | the capsular polysaccharide from streptococcus pneumoniae type 27 (s-27) has been fragmented into oligosaccharides by controlled degradations. binding of these derived small ligands with heterogeneous rabbit anti-s-27 antibody of selected phosphorylcholine affinity indicates that a major immunodeterminant of s-27 is the phosphorylcholine moiety, but that these anti-pc. antibodies are also capable of binding to the polysaccharide backbone from which the p.c. has been removed. | 1979 | 87460 |
| the patterns and distribution of euchrysine-binding grains in nonstimulated human peripheral t and non-t lymphocytes. | | 1979 | 86386 |
| [current status of chemotherapy in malignant head and neck neoplasms (proceedings)]. | | 1979 | 84806 |
| effect of quantity and quality of igg antibodies on blocking of allergenic histamine release in vitro. | in a study of the protective role of igg antibodies in allergen injection therapy, serum antibodies to timothy grass allergens were raised in rabbits and tested for inhibition of histamine release from basophils of grass pollen-sensitive human subjects. rabbit sera were not inhibitory until late in the immune response when large amounts of relatively low affinity igg antibodies were detected by primary binding. inhibition of histamine release by late sera required preincubation of allergen and a ... | 1979 | 84798 |
| guinea pig transfer factor: restriction of macrophage migration inhibitory activity to extraordinary dilution confirmed with demonstration of diminished potency of stored preparations. | | 1979 | 88292 |
| ultrastructural localization of calcium around the membrane of the surface connected system in the human platelet. | the localization of calcium in the membrane system of human platelets was determined by ultrahistochemical methods equipped with an electron probe x-ray microanalyzer. after potassium oxalate-glutaraldehyde treatment large amounts of electron opaque precipitates were observed around the membrane of the surface connected system. electron probe x-ray microanalysis clearly defined that the precipitates were composed of calcium oxalate. the localization of calcium on the membrane of the surface conn ... | 1978 | 96043 |
| the relative biological effectiveness of pions in the acute response of human skin. | | 1977 | 96068 |
| endemic pneumocystis carinii in a marmoset colony. | | 1978 | 96156 |
| use of antibodies for studying the sidedness of membrane components. | | 1979 | 88659 |
| the influenza virus: antigenic composition and immune response. | the architecture and chemical composition of the influenza virus particle is described with particular reference to the protein constituents and their genetic control. the dominant role in infection of the surface proteins - haemagglutinins and neuraminidases - acting as antigens and undergoing variation in time known as antigenic drift and shift is explained. the immuno-diffusion technique has illuminated the interrelationships of the haemagglutinins of influenza a viruses recovered over long p ... | 1979 | 88726 |
| reverse transcriptase-associated rnase h. part iv. pyrophosphate does not inhibit rnase h activity of amv dna polymerase. | | 1979 | 93479 |
| purification of myelin carbonic anhydrase. | | 1978 | 97371 |
| effects of pelleting and sterilization of diet on 2 strains of rabbit coccidia. | both autoclaving and irradiation at levels currently used for other laboratory animal diets appear to be satisfactory for the elimination of 2 of the most robust and pathogenic species of rabbit coccidia. pelleting by the usual methods, even at 68 degrees c, was found to be unsatisfactory in eliminating all oocysts from heavily infected diet. | 1978 | 97471 |
| comutagenicity, competitive enzyme substrates, and in vitro carcinogenicity assays. | | 1978 | 97523 |
| guinea pig immunoglobulin light chain isotypes. i. separation of kappa and lambda chains and the identification of three isotypes of the lambda chain constant homology region. | the separation of intact kappa chain and two fragments comprising lambda (lambda) chain from immunoglobulin light chain pools isolated from strain 13 guinea pigs is achieved by cyanogen bromide digestion and gel filtration before and after reductive clevage of disulfide bonds. the smaller lambda chain fragment derives from the original carboxyl-terminus of lambda chain. the partial sequence of component tryptic and thermolytic peptides of this thirty-nine residue fragment allowed its complete se ... | 1978 | 103729 |
| congenital defects due to hyperthermia. | | 1978 | 104560 |
| ophthalmic features of chromosome deletion 4p- (wolf-hirschhorn syndrome). | by using the giemsa banding technique we identified three patients with chromosome deletion 4p-. all had anterior segment anomalies, exotropia, blepharoptosis, antimongoloid palpebral fissures, hypertelorism, and disk abnormalities. one patient (case 1) had rieger's anomaly. some clinical features in patients with 4p- are similar to those in patients with chromosome deletion 5p-, cri-du-chat syndrome, although 4p- individuals do not have the distinctive cry. the ocular features which distinguish ... | 1978 | 104624 |
| [investigations of the incidence of neisseria gonorrhoeae on the pharyngeal mucosa (author's transl)]. | | 1978 | 98235 |
| specific labelling of replicating spp1 dna: analysis of viral dna synthesis and identification of phage dna-genes. | specific labelling of replicating bacteriophage spp1 dna can be achieved by infection at nonpermissive temperature of a b. subtilis strain carrying the initation mutation dnab ts134. under these conditions host dna synthesis is reduced by 90 to 95%. this technique was used to identify cistrons of spp1 involved in phage dna synthesis and to define intermediates in spp1 replication. | 1978 | 105242 |
| interference of iodine-125 ligands in radioimmunoassay: evidence implicating thyroxine-binding globulin. | although there is abundant published evidence that radioiodinated antigens interfere in digoxin radioimmunoassays, other radioimmunoassays are similarly affected. we investigated the relationship of radioiodinated antigen structure to its function in the immunoassay. carrier-free 125i-labeled iodotyrosine and iodohistamine derivatives were incubated with human serum, and the bound and free fractions were separated. we demonstrated that diiodotyrosyl analogs bind avidly to serum proteins. because ... | 1979 | 108034 |
| vaginal discharge and sexually transmitted diseases. | | 1978 | 108216 |
| electron microscopic study of bacillus subtilis protoplast fusion. | when protoplasts derived from sporulating cells of bacillus subtilis were fused by exposure to polyethylene glycol (peg) and fixed immediately thereafter, protoplasts with two enclosed prespores could be seen by electron microscope. the number of fusion events was greatly increased, and multiply fused protoplasts appeared, when the peg-treated suspension was diluted in hypertonic broth and reincubated before fixation. this post-peg incubation effect is taken to indicate a fusion mechanism of two ... | 1979 | 108247 |
| thyrotropin-releasing hormone is not required for thyrotropin secretion in the perinatal rat. | to determine the role of thyrotropin-releasing hormone (trh) in the regulation of thyroid-stimulating hormone (tsh) secretion in the perinatal period, a physiological approach of neutralizing circulating trh in the fetal and early neonatal rat was employed. trh-antiserum (trh-as) raised in rabbits and administered daily to low iodine-propylthiouracil (lid-ptu)-fed pregnant rats from days 12 to 19 of gestation markedly impaired the rise in serum tsh to lid-ptu when compared with normal rabbit ser ... | 1979 | 108290 |
| an explosive outbreak of influenza caused by a/ussr/77-like virus on a united states naval ship. | | 1979 | 119926 |
| trna gene clusters in escherichia coli dna. | | 1975 | 127650 |
| morphology, surface markers, and in vitro responses of a human leukemic t cell. | | 1975 | 127678 |
| inhibitory effects of mersalyl and of antibodies directed against smooth-muscle myosin on a calcium adenosine triphosphatase of the plasma membrane from mouse liver cells. | | 1976 | 130830 |
| bronchial spasmolytic action of terbutaline and fenoterol compared with that of ipratropium bromide in the anaesthetized dog. | continuous recordings of heart rate, respiration rate, minute volume and bronchial resistance were made over a period of altogether three hours in spontaneously breathing dogs anaesthetized with chloralose. at hourly intervals, before and 1 and 2 hr after the administration of bronchodilators, bronchial spasm was induced by inhalation of an acetylcholine solution. two beta2-stimulants, terbutaline and fenoterol, were administered by inhalation in doses of 0.5, 0.16 and 0.05 mg/kg and 0.1, 0.3 an ... | 1978 | 152096 |
| [intravenous cholangiography in hyperbilirubinaemia (author's transl)]. | the effect of bilirubin on biliary and renal excretion of methylglucamine-iodipamide at an infusion rate of 1.3, 2.6 and 5.2 mu mol/min/kg was studied by intravenous injection into five dogs with normal liver functions and chronic duodenal fistulas. unconjugated bilirubin administered in a dose of 0.1 mu mol/min/kg reduced biliary iodipamide excretion and concentration significantly, but did not affect renal contrast material excretion. this indicates that the unsatisficatory radiological result ... | 1979 | 153309 |
| immunologic aspects of 1, 2-dimethylhydrazine-induced colon tumors in rats. | investigation of immunologic aspects of colon tumors induced in rats by 1,2-dimethylhydrazine demonstrated that: 1) an antigen in an extract of colon tumors was not detected in the normal colon. it was related to antigens in rat fetuses and did not cross-react immunologically with carcinoembryonic antigen. 2) mucinous adenocarcinomas of the colon were significantly associated spatially with lymphoid follicles. | 1975 | 165314 |
| [persistence of latex-agglutination inhibiting and neutralizing antibodies against enterovirus in the healthy population (author's transl)]. | sera of 122 persons were examined by the neutralization and latex-agglutination inhibition tests for antibodies against polio type i, coxsackie virus type a 9 and b 4, as well as echo virus type 9. with increasing age there was a titre difference between latex-agglutination inhibiting and neutralizing antibodies, due to the earlier fall in the former. these antibodies, contrary to neutralizing ones, were in the lower titre region. the results confirmed the usefulness of the latex-agglutination i ... | 1975 | 169112 |
| steroid production by the isolated rabbit ovarian follicle. | graafian follicles from new zealand white rabbits were incubated at 37 degrees c for various periods of time with air as the gas phase. media were changed every 15 min and stored at -15 degrees c until analysed for progestins, 17 beta-hydroxyandrogens and oestrogens using established radioimmunoassay procedures. at fixed times after the start of the incubations, media containing various test substances were added with subsequent replacement by medium alone. addition of 5 mug lh/ml for 1 sec caus ... | 1975 | 173845 |
| malignant transformation-linked imbalance: decreased xanthine oxidase activity in hepatomas. | xanthine oxidase was decreased 2- to 10-fold in all examined rat hepatomas irrespective of the malignancy; growth rate and degrees of histological differentiation of the neoplasms. the affinity to substrate (km=6-8 mum) and the ph optimum (8.0) of the liver and hepatoma enzymes were the same. the reprogramming of gene expression, as manifested in the decreased activity of this key purine metabolizing enzyme, appears to be specific to neoplastic transformation. since glutamine prpp amidotransfera ... | 1975 | 179860 |
| human very low density lipoproteins stimulate triglyceride synthesis in rat adipose cells. | | 1979 | 219401 |
| transformation by reticuloendotheliosis virus: development of a focus assay and isolation of a nontransforming virus. | | 1979 | 219596 |
| microassay of cylic nucleotides in vessel wall. i. cyclic amp. | | 1976 | 186301 |
| serum angiotensin-converting enzyme, transcobalamin and lysozyme in normal and lepromatous armadillos. | | 1979 | 229221 |
| in vitro cultivation of anaplasma marginale: growth pattern and morphologic appearance. | anaplasma marginale propagated in vitro showed an increasing rate of replication in a sequence of three experiments. the changes in the percentage of parasitized erythrocytes and identification of the organism in culture were monitored by the giemsa-staining and the direct fluorescent antibody techniques. the ultrastructure of the organism in culture also was determined. the percentage of parasitized erythrocytes increased more than three times in the first experiment during a period of 8 days, ... | 1979 | 230766 |
| comparison of the peroxidatic activity of cytochrome p-450 with other hemoproteins and model compounds. | the h2o2 dependent catalysis of cytochrome p-450 was compared with the catalytic mechanism of horse radish peroxidase, methemoglobin and iron protoporphyrin complexes. a relatively stable intermediate being comparable to compound i of horse radish peroxidase is formed in the case of iron porphyrin complexes, methemoglobin and probably cytochrome p-450. in the case of peroxidase compound ii is the more stable intermediate. this could be the reason for the different catalytic properties of peroxid ... | 1979 | 229683 |
| exit of anaplasma marginale from bovine red blood cells. | incubation of anaplasma marginale-infected bovine red blood cells in a flow culture system resulted in a decrease of observable marginal bodies. the decrease was twice the degree of hemolysis, indicating that marginal bodies may leave red blood cells without concomitant lysis of the host cell. | 1975 | 237446 |
| preparation and properties of 3-halopyridine--adenine dinucleotides, nad+ analogues and model compounds. | the preparation of model compounds 1-(2',6'-dichlorobenzyl)-3-halogenopyridinium and the study of their properties were achieved. their chemical reduction to the corresponding 1,4-dihydropyridines is proved by spectroscopic analysis. 3-iodopyridine--adenine dinucleotide was prepared by enzymic transglycosidation while the 3-chloro, 3-bromo and 3-iodo pyridine--adenine dinucleotides were synthesized from 3-amino-pyridine--adenine dinucleotide. the 3-halogenopyridine--adenine dinucleotides were pr ... | 1976 | 179812 |
| the influence of diazepam on the dosage of muscel-relaxants during anaesthesia. | the literature on anaesthesiological studies performed to investigate a possible sparing effect of diazepam on the dosage of muscle-relaxants during anaesthesia is reviewed. despite some conflicting results it can be concluded that diazepam exerts such an effect, thus allowing between 8 and 30% of the amounts of muscle-relaxants, depending on the type of anesthesia used, to be spared. this conclusion is also supported by the results of pharmacological studies in animals, using the head drop meth ... | 1975 | 165742 |
| the nature of the protein moieties of cartilage proteoglycans of pig and ox. | proteoglycans extracted with 4m-guanidinium chloride from pig laryngeal cartilage and bovine nasal septum were purified by density-gradient centrifugation in cscl under 'associative' followed by 'dissociative' conditions [hascall & sajdera (1969) j. biol. chem. 244, 2384-2396]. proteoglycans were then digested exhaustively with testicular hyaluronidase, which removed about 80% of the chondroitin sulphate. the hyaluronidase was purified until no proteolytic activity was detectable under the condi ... | 1975 | 128355 |
| flaujeac trait. deficiency of human plasma kininogen. | flaujeac trait plasma resembled hageman trait or fletcher trait, in that the intrinsic coagulation pathway, plasma fibinolytic pathway, kinin-forming system, permeability factor of dilution (pf/dil) phenomenon were abnormal. the defect in each assay was reconstituted by afactor separable from hageman factor or fletcher factor. this substance was an alpha-globulin with an approximate mol wt of 170,000. flaujeac plasma did not release a kinin upon incubation with kallikrein and was deficient in to ... | 1975 | 127805 |
| electron transport chain from glycerol 3-phosphate to nitrate in escherichia coli. | it is known that in escherichia coli two dehydrogenases of the flavoprotein kind can participate in the transfer of hydrogens from sn-glycerol 3-phosphate (g3p) to nitrate and that possession of either enzyme is sufficient to permit anaerobic growth on glycerol as carbon source and nitrate as hydrogen acceptor. results from this study show that under such a growth condition a protein with light-absorption characteristics of cytochrome b1 is induced. if g3p, nitrate, and adenosine diphosphate are ... | 1975 | 127786 |
| detection of antibodies in trypanosome-infected cattle by means of a microplate enzyme-linked immunosorbent assay. | a micromodification of the enzyme-linked immunosorbent assay (elisa) was evaluated for its potential application in the immunodiagonsis of bovine trypanosomiasis. serum samples from infected and non-infected zebu cattle and samples from friesian cattle with experimental infections were exmained for the presence of trypanosomal antibodies. there were significant differences between the microelisa values obtained with samples from infected and non-infected cattle. during the course of infection mi ... | 1977 | 333677 |
| the mechanism of trapping of immune complexes in experimental antigen-induced arthritis. | the role of acute inflammation and of specific antibody in the retention of antigen in joint collagenous tissues of immunized rabbits was examined. the role of the acute synovitis occurring immediately after ia antigen injection in the retention of immune complexes was investigated by the concomitant ia injection of trace amounts of 1258-bsa and mono-sodium urate crystals and by the production of an acute arthus reaction using an unrelated antigen in doubly immunized rabbits. in neither case was ... | 1975 | 128111 |
| immunobiologic factors in the pathogenesis of renal infection. | | 1979 | 161989 |
| streptomycin sensitivity of ribosomes isolated from a streptomycin-producing streptomyces griseus. | the streptomycin sensitivity of ribosomes derived from a streptomycin-producing streptomyces griseus was examined in a polyuridylic acid directed 14c-phenylalanine incorporating system. in order to get reproducible results it is essential to use cell-free extracts which do not inactivate streptomycin. this condition can be fulfilled by the combination of washed ribosomes of the streptomycin-producing strain and the 110 000 g supernatant of the streptomycin-nonproducing variant of s. griseus, bec ... | 1979 | 120678 |
| experimental nasopharyngitis and pneumonia caused by chlamydia trachomatis in infant baboons: histopathologic comparison with a case in a human infant. | three infant male baboons were inoculated with a strain of chlamydia trachomatis isolated from a human infant with pneumonitis. one baboon, inoculated by intratracheal, nasopharyngeal, and oropharyngeal seeding, had rales, radiographic evidence of pneumonia, persistent nasopharyngeal c. trachomatis infection, and a four-fold rise in titer of antibody. at sacrifice 24 days after inoculation, nasopharynx, trachea, airways, and lung yielded c. trachomatis, and epithelial inclusions were seen by lig ... | 1979 | 108341 |
| epizootiology and control of anaplasmosis in south africa. | the history of bovine anaplasmosis, or tick-born gallsickness, since the discovery of anaplasma marginale by sir arnold theiler is briefly reviewed. the development of the anaplasma centrale vaccine by theiler, up to the composition of the present vaccine issued by onderstepoort in which the original isolate is still passaged, is discussed in detail. recent transmission studies at onderstepoort have shown that 5 tick species are capable of transmitting anaplasmosis intrastadially, and intrastadi ... | 1979 | 399978 |
| myeloma proteins from nzb and balb/c mice: structural and functional differences. | structural and functional analyses of myeloma immunoglobulins from inbred balb/c mice and humans have provided important insights into the structure of the antibody molecule and the expression and evolution of antibody genes. one important question concerning these analyses is whether the myeloma process selects, in a nonrandom manner, the lymphocytes to be transformed. the availability of myeloma tumors in a second inbred strain of mouse, nzb, permits us to approach this question. in this respe ... | 1979 | 108679 |
| 3'-terminal nucleotide sequence of alfalfa mosaic virus rna 4. | the sequence of the 3'-terminal 91 nucleotides of alfalfa mosaic virus rna 4, the messenger for the viral coat protein, has been elucidated. a fragment containing the 3' terminus of the rna was obtained by mild digestion with rnase t1. the primary structure of the fragment was deduced by labeling it in vitro at its 5' terminus and application of rna sequencing techniques. the sequence is completely extracistronic and is believed to contain the binding sites for the viral coat protein and replica ... | 1979 | 108677 |
| seasonal variation and the influence of body temperature on plasma concentrations and binding of thyroxine and triiodothyronine in the woodchuck. | woodchuck plasma was collected during four seasons of the year and assayed for total and dialyzable (free) t4 and t3 and for rt3. plasma concentrations of total and free t4 and t3 were higher in the spring (t4, 5.4 +/- 0.6 microgram/dl; free t4, 3.0 +/- 0.4 ng/dl; t3, 202 +/- 22 ng/dl; free t3, 0.51 +/- 0.04 ng/dl) and lower in the prehibernatory fattening period in summer (t4, 2.3 +/- 1.0 microgram/dl; free t4, 1.2 +/- 0.5 ng/dl; t3, 45 +/- 27 ng/dl; free t3, 0.16 +/- 0.10 ng/dl) and fall (t4, ... | 1979 | 108089 |
| evaluation of the anaplasmosis rapid card agglutination test for detecting experimentally-infected elk. | anaplasma marginale was experimentally transmitted from cattle to elk to cattle. six intact adult elk (cervus canadensis canadensis) inoculated with freshly collected heparinized blood from cattle chronically infected with a. marginale became asymptomatic carriers. although the elk did not develop clinical or hematologic evidence of infection, they became seropositive by the serum(srca) and plasma rapid card agglutination (prca) tests. blood from the experimentally-infected elk produced disease ... | 1979 | 501840 |
| evaluation of a new long-acting oxytetracycline formulation against anaplasmosis in colombian cattle. | a long-acting injectable (terramycin/la) forulated to contain 200 mg/ml of oxytetracycline was tested and compared to a commercially available oxytetracycline injectable (emicina) containing 50 mg/ml in the treatments of experimentally induced anaplasma marginale infection in colombian cattle. group 1, consisting of 10 infected calves, served as non-treated controls. ten infected calves (group 2) were treated with two doses of emicina, each at 10 mg/kg, intramuscularly (im), and another ten infe ... | 1979 | 483386 |
| in vitro cultivation of anaplasma marginale: invasion of and development in noninfected erythrocytes. | ovine erythrocytes infected with attenuated anaplasma marginale organisms were cultured in a suspension of normal ovine erythrocytes and normal bovine erythrocytes for 42 days. in each system, the organism showed an initial period of rapid growth followed by a gradual decrease in the percentage of parasitized erythrocytes accompanied by cyclic peaks. the percentage of infection of ovine erythrocytes were not different when normal ovine or bovine erythrocytes were added to the cultures. in vitro ... | 1979 | 525898 |
| evaluation of the anaplasmosis rapid card agglutination test for detecting experimentally infected elk. | anaplasmosis was experimentally transmitted from cattle to elk to cattle. six non-splenectomized adult elk (cervus canadensis canadensis) that were inoculated with freshly collected heparinized blood from cattle chronically infected with anaplasma marginale became asymptomatic carriers. although the exposed elk did not develop clinical or hematologic evidence of infection they become seropositive by the serum and plasma rapid card agglutination (rca) tests. blood from the experimentally infected ... | 1979 | 527345 |
| reticulo-rumen motility in the chronically vagotomized sheep [proceedings]. | | 1979 | 529098 |
| voltage-clamp studies on the canine purkinje fibre [proceedings]. | | 1979 | 529143 |
| some observations on the effects of magnesium on the isolated perfused rat mesentery [proceedings]. | | 1979 | 529150 |
| [preparation and comparative testing of antigens for the immunodiagnosis of anaplasmosis in cattle]. | three antigen types were produced by ultra-sound treatment of the initial material (anaplasma marginale theiler infected blood) and its subsequent sedimentional centrifugation from 10 000 to 100 000 g. their specificity and susceptibility to the reaction complement binding (rcb) and to the capillary agglutination (ca) serological tests was comparatively checked up. antigens a and b were very active in rcb, while antigen b--in the ca reaction. by these qualities they meet the requirements and are ... | 1979 | 545841 |
| [the experimental transmission of anaplasma marginale by the brown dog tick rhipicephalus sanguineus in australia]. | | 1979 | 539952 |
| serial prolactin and thyrotropin responses to thyrotropin-releasing hormone throughout normal human pregnancy. | | 1979 | 107178 |
| [immuno-electro-diffusion on cellulose acetate membrane for serological testing in human malaria (author's transl)]. | an immuno-electro-diffusion assay for human malaria, using cellulose acetate gel, is described. more than 3500 sera have been tested by this method, which proved very useful for epidemiology since 400 sera a day can be tested by one person, with simple equipment. its sensitivity compared well to the ifa test, although the antibodies revealed are quite different. a constnat precipitin line appeared, against 56 degrees c labile antigens, in sera of subjects infected by p. falciparum, and up to 8 o ... | 1978 | 98748 |
| the use of a short and a long acting oxytetracycline for the treatment of anaplasma marginale in splenectomized calves. | twenty splenectomized afrikander/simmentaler cross animals were used. eight of these were naturally infected with anaplasma marginale while 12 were infected artificially. when the packed cell volume was reduced to between 24% and 20% during the post splenectomy relapses or primary infections, six animals were treated with a single injection of 20 mg/kg of long acting oxytetracycline (la) and seven animals were treated on two successive days with 10 mg/kg of short acting oxytetracycline (sa). the ... | 1979 | 551196 |
| comparative efficacy of drugs in bovine anaplasmosis. | calves experimentally infected with anaplasma marginale were subjected to chemotherapeutic treatment in groups, each group receiving one drug only. observations were made on clinical signs, haematological changes, parasitaemias and levels of antibodies as measured by the complement fixation test. the most effective treatment was with imidocarb; other drugs which were apparently effective were steclin, terramycin and aureomycin in that order, while chloroquin diphosphate and camoquin gave little ... | 1979 | 552672 |
| induction of arylhydrocarbon hydroxylase and udp glucuronosyl transferase by pcb in hepatic cell cultures. | the purpose of the investigation was to derive a hepatic cell line to be used in induction studies of drug metabolizing enzymes. two pure cell lines were isolated from primary liver cell cultures, one with an epithelial-like appearance, the other with a fibroblast-like appearance. the specific activities of arylhydrocarbon hydroxylase (ahh) and udp glucuronosyl transferase (udpgt) were greater in hepatocyte cultures than in primary cultures or in fibroblast cultures. pcb enhanced the activity of ... | 1978 | 98133 |
| [aspects of the biochemistry of the urine in a newborn infant with "typical" valinuria and the effect of ingestion of valine on amino acid transport at the level of the brain in animals]. | | 1978 | 97690 |
| comparison of kinetic and end-point diffusion methods for quantitating human serum immunoglobulins. | proficiency testing results were used to compare the kinetic and end-point versions of the single radial immunodiffusion method for quantitating human serum immunoglobulins. statistical analysis of the results indicated that the results were not normally distributed but that the log normal distribution gives the best fit of any of the well-known frequency distributions. consequently, statistical analysis of immunoglobulin results must be log transformed before parametric statistical tests can be ... | 1978 | 97309 |
| amylase levels in the tissues and body fluids of the domestic cat (felis catus). | 1. the amylase activities of serum, liver, pancreas, submaxillary and parotid glands, saliva, duodenum, colon, lung, heart, spleen, kidney and skeletal muscle of the domestic cat, were determined. 2. as in most mammals, the highest amylase content was found in the pancreas. levels in saliva and salivary glands were very low. 3. amylase activities of the serum, liver and pancreas have an optimum ph 7.0-7.5. 4. amylases of serum, liver and pancreas were inactivated by removal of chloride and these ... | 1979 | 95689 |
| iatrogenic transmission of anaplasma marginale in beef cattle. | | 1977 | 586056 |
| studies on mononuclear phagocyte progenitor cells: morphology of cells and colonies in liquid culture of mouse bone marrow. | | 1979 | 88518 |
| cyclic thrombocytopenia induced by a rickettsia-like agent in dogs. | hematologic manifestations and the ultrastructure of a platelet-specific microorganism isolated from a dog in florida were studied. the agent was readily transmitted experimentally to adult dogs by intravenous inoculation with infected blood. parasitemias and concomitant thrombocytopenias were cyclic in that both recurred within relatively constant periods of one to two weeks following experimental infections. hemorrhage was not a manifestation of the disease even though thrombocytopenias were s ... | 1978 | 627738 |
| fatal adenovirus infection with misleading positive serology for infectious mononucleosis. | symptoms and serological tests in a 19-year-old man with pneumonia indicated that he had infectious mononucleosis. adenovirus type 7 was demonstrated in the lung by electron microscopy and was cultured from the liver, lung, lymph-nodes, and kidney. | 1979 | 84950 |
| fluorescein diacetate staining of anaplasma marginale as a possible measure of viability. | fluorescein diacetate viability-staining technique was applied to anaplasma marginale. marginal bodies took up the stain and became fluorescent. when infected red blood cells were incubated at 55 c, the number of fluorescent anaplasma decreased at a rate similar to the known loss of viability. | 1978 | 629472 |
| changes in serum concentration of phagocytosis-stimulating factor in experimentally induced bovine anaplasmosis: preliminary findings. | to determine whether correlation exists between serum concentration of phagocytosis-stimulating factor (psf) and in vivo phagocytic activity, 2 splenectomized steers were inoculated with anaplasma marginale, and their serum psf concentrations were monitored. at the time of the anemic crisis, serum psf concentrations were elevated five to tenfold. | 1976 | 58572 |
| [characteristics of sepiapterin reductase from tawa and yoshida sarcomas (author's transl)]. | | 1978 | 228781 |
| [study of cytoplasmic rna in tissues of the rat, regenerating liver and in liver of rats with hepatoma pc-1]. | cytoplasmic rna of the rat regenerating liver and liver of rats with hepatoma pc-1 is separated into five fractions on the column of kieselgur with methylated albumin (mak). studies in the nucleotide composition showed that certain fractions of cytoplasm rna are ribosomal and messenger rna. the highest amount of radioactive precursor is incorporated into messenger rna of the rat regenerating liver and liver of the rats with hepatoma pc-1 which is eluated from the column by 0.2% solution of sodiu ... | 1977 | 201068 |
| structural studies and physical maps for the herpes simplex virus genome. | | 1977 | 193820 |
| contraction of glycerinated muscle fibers as a function of the atp concentration. | we have measured the force-velocity curves of glycerinated rabbit psoas fibers over a range of atp concentration from 2.5 microm to 5 mm. as the atp concentration is increased, the isometric tension increases to a maximum around 50 microm, then decreases to a plateau at 70% of the maximum by 1 mm atp. at low atp concentrations the maximum velocity of contraction is low and increases with increasing atp, reaching a plateau at approximately 2 lengths per second by 1 mm atp. our studies suggest tha ... | 1979 | 262549 |
| survey of the incidence of anaplasmosis among nigerian zebu trade cattle. | sera samples from 573 nigerian zebu trade cattle were evaluated by the rapid card agglutination test for antibodies to anaplasma marginale between may and july 1977. the results showed 34 per cent reactors as against 66 per cent negatives. the above results showed a significant level of exposure to the infection among the fulani cattle managed under a husbandry system in which there is no provision for tick control. the significance of this level of exposure to anaplasma marginale in relation to ... | 1978 | 664019 |
| electrophoresis of lysozyme into microscoccus-containing agarose gel: quantitative and analytical applications. | electrophoresis of lysozyme into agarose gel containing micrococcus lysodeikticus causes lysis of the microorganism, allowing the development of two methods, one for quantitation ("lyso-rocket electrophoresis") and the other for electrophoretic characterization ("crossed lyso-electrophoresis") of lysozyme. the lyso-rocket technique provides an alternative to the method currently used for quantitative assay. by the use of crossed lyso-electrophoresis we have provided evidence, for the first time, ... | 1977 | 265197 |
| locomotion of human lymphoid cells. i. effect of culture and con a on t and non-t lymphocytes. | | 1977 | 303147 |
| evidence for the maintenance of motoneurone properties by muscle activity. | 1. electrophysiological properties of soleus motoneurones in adult cats were examined with intracellular electrodes following alterations of activity of the soleus muscle induced by transection of the thoracic spinal cord or by conduction block of the muscle nerve with tetrodotoxin (ttx) cuffs. attempts were also made to maintain muscle activity by daily stimulation of the maintain muscle activity by daily stimulation of the peripheral nerve. 2. within 8 days after transection of the thoracic co ... | 1978 | 279668 |
| absence of increasing incidence of meningitis caused by haemophilus influenza type b. | an epidemiologic survey of meningitis caused by haemophilus influenzae type b in children aged zero to four years during an 11-year period (january 1965-december 1975) was conducted in the baltimore, maryland, metropolitan area to examine recent trends in the incidence of this disease. cases of h. influenzae meningitis were identified at all 19 hospitals in the city and county of baltimore and all 41 hospitals in the surrounding area. the population at risk (age, zero to four years) was estimate ... | 1979 | 317292 |
| methods to improve the health of cattle in the tropics: conclusions and economic appraisal. | the results of the immune responses of immunised and chemoprophylactically treated calves to tick-borne (boophilus microplus) challenge indicate that the system of immunisation was effective in protecting cattle against anaplasma marginale, babesia argentina (bovis), and b. bigemina. however, chemoprophylaxis was effective only against babesia spp. but not against a. marginale. both methods showed a substantial advantage over no control system when using native cattle breeds in a zone endemic fo ... | 1978 | 705891 |
| epidemiology of cattle anaplasmosis in colombia: i. prevalence and distribution of agglutinating antibodies. | a survey to detect antibodies against anaplasma marginale, using the card test, was conducted on serum samples collected from cattle of cordoba department and north-eastern region of the department of antioquia in the republic of colombia. the samples were from native cattle of spanish descent (bos taurus), zebu (bos indicus) and various zebu-native crosses. the age range was between three and eight year olds. the results revealed that 90.3% of the samples were positive. all breeds showed a high ... | 1978 | 705897 |
| experimental bovine anaplasmosis: clinico-pathological and nutritional studies. | groups of brahman-cross steers maintained on two nutritional planes were infected intravenously with a stabilate of anaplasma marginale. in general, animals on the higher plane of nutrition were more severely affected. fever was the first clinical sign of anaplasmosis but, like anaemia, was absent in the mildest cases. when present anaemia appeared two to three weeks after infection. there was a corresponding increase in erythrocyte sedimentation rates when read after 24 h but not at 1 h. the ha ... | 1978 | 705052 |
| comparisons of the complement-fixation, indirect fluorescent antibody, and card agglutination tests for the diagnosis of bovine anaplasmosis. | results of complement-fixation (cf), indirect fluorescent antibody (ifa), and card agglutination (ct) tests were statistically compared, using 380 serum samples obtained from 140 cattle which were disease-free or naturally or experimentally infected with anaplasma marginale of colombian origin. the ifa test was significantly the most sensitive for detection of amimals infected with anaplasmosis (97%); the ct test and the cf test were less so (84% and 79%, respectively). however, the most efficie ... | 1978 | 358874 |
| a comparison of 4 serological tests in the detection of humoral antibodies to anaplasmosis in cattle. | a capillary agglutination (ca), a complement fixation (cf), a plate agglutination (pt) and an indirect fluorescent antibody (ifa) test to detect humoral antibodies to anaplasma marginale are described. serums from 3, 4 or 5 groups of cattle were used to examine the efficiency of the tests. agreement between all 4 tests was 86.6%. agreement between pairs of tests was greater. the cf test was the most sensitive while the pt test was the least sensitive. however the pt could be carried out very rap ... | 1978 | 363118 |
| quantitative estimation of leishmanial antibody titers by enzyme-linked immunosorbent assay. | the response to immunization against leishmania braziliensis in rabbits was followed by measurement of antibody by enzyme-linked immunosorbent assay (elisa), passive hemagglutination, complement fixation, and countercurrent immunoelectrophoresis. passive hemagglutination and complement-fixation titers were interpreted according to standard methods, whereas elisa exact titers were derived by the equation y = a-b log x, which describes the straight line that results when absorbances are plotted ag ... | 1979 | 387886 |
| social organization and food resources availability in primates: a socio-bioenergetic analysis of diet and disease hypotheses. | data obtained during a field study of two species of nonhuman primates (alouatta villosa and ateles geoffroyi) living in the tikal national park in guatemala are used to suggest an answer to the question: to what extent is the existence of a particular form of social organization (group size, structure, and composition) an indication of the amount of energy in the form of food resources available to animals in a particular habitat? seven researchers working in teams spent 2,318 hours in the fiel ... | 1977 | 403771 |
| electron microscope studies of anaplasma marginale in an aedes albopictus culture system. | anaplasma marginale was seen by electron microscopy within the cytoplasm of aedes albopictus cells in culture. anaplasma marginale was seen in free bovine rbc as well as in rbc phagocytized by ae albopictus cells. some a marginale organisms, both intracellular and extracellular, remained intact for as long as 60 days in culture, and anaplasma inclusion appendages were seen in free-lysed rbc and in lysed rbc phagocytized by ae albopictus cells. although some a marginale organisms had deteriorated ... | 1979 | 525907 |
| amicarbalide: a therapeutic agent for anaplasmosis. | when administered subcutaneously in 2 equal daily doses at a total dosage rate of 20 mg/kg, amicarbalide was found to be an effective agent for controlling acute infections of anaplasma marginale and a. centrale in intact and splenectomized cattle. attempts to sterilize patent and latent anaplasma infections, however, were unsuccessful. at total dosage rates of 40 mg/kg and higher, amicarbalide exhibited potent hepato- and nephrotoxic tendencies. | 1978 | 750960 |
| studies on theileriidae (sporozoa) in tanzania. viii. experiments with african buffalo (syncerus caffer). | it has been found that african buffalo may remain infective carriers of theileria parva lawrencei for at least 5 years. this infection is now known to exist in buffalo in 3 sites in northern tanzania. it was shown that buffalo can be infected with haematoxenus veliferus and theileria mutans of cattle and retransmission of these parasites from buffalo to cattle was successful. the species of haematoxemus reported in wild buffalo in central and east africa is likely to be h. veliferus, while the p ... | 1977 | 410128 |
| characterization of the inclusion limiting membrane of anaplasma marginale by immunoferritin labeling. | purified anti-erythrocytic membrane antibody (pama) was prepared from rabbit anti-bovine erythrocyte serum by an adsorption and elution technique, utilizing bovine erythrocytes. lysed and washed anaplasma-infected erythrocytes were incubated with pama or control reagents. specimens were then subjected to immunoferritin labeling with ferritin antiglobulin conjugate. upon examination by electron microscopy, specimens incubated with pama showed heavy ferritin labeling of erythrocytic membranes and ... | 1979 | 475129 |
| [existence of glucocorticoid-specific receptors in the plasma membranes of rat liver]. | it was found that isolated plasma membranes whose purity was assayed by determinations of marker enzyme activities, specifically bind dexamethasone. the association constant and the number of binding sites were found to be equal to (7,03 +/- 4,05) . 10(9) m-1 and (1,6 +/- 0,18) . 10(-14) mol/mg protein, respectively. it was assumed that lipoprotein components of plasma membranes are involved in this binding. | 1979 | 546445 |
| resistance to anaplasmosis after elimination of latent anaplasma marginale infections. | ten 30-month-old cattle (group 2) were inoculated with anaplasma marginale 6 months after the latent a marginale infection was eliminated by oral chlortetracycline therapy. in addition, 4 cattle (group 1) with no record of infection and 4 cattle (group 3) of equivalent age previously vaccinated with a killed antigen were similarly inoculated. moderate to severe clinical signs of acute anaplasmosis occurred in the group 1 (previously uninfected) cattle, whereas only 1 of the chlortetracycline-tre ... | 1977 | 557302 |
| bovine anaplasmosis transmission studies conducted under controlled natural exposure in a dermacentor andersoni = (venustus) indigenous area of eastern oregon. | in anaplasma marginale transmission studies conducted on the high semi-arid range of eastern oregon during the 1974 and 1975 vector season, a marginale-susceptible calves (principals) were maintained on 2 raised tick-proof platforms. anaplasmosis-susceptible control calves of approximately the same age and latent-infected cows grazed the area surrounding the platforms. one latent-infected steer spent the entire 1975 vector season on a platform with the principals. the 28 principals did not devel ... | 1977 | 557939 |