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effect of physical and chemical factors on pasteurella multocida bacteriophages.studied was the effect of some physical factors (temperature, ph of various values) and some chemical ones (urea, sodium citrate, sarcolysin, methylene blue, acridine orange, pyronin, crystal violet, potassium permanganate, fuchsin, tetracycline and tetraolean) on phages specifically lysing pasteurella multocida strains, having the following signature: 3, 4, 6, 22, 32, 115, 967, 1075, and c-2. all investigated phages showed signs of inactivation at 48 degrees c with a further linear dependence, ...19768867
hormonal control of zinc uptake and binding in the rat dorsolateral prostate.the zinc uptake in the dorsolateral prostate of rats was studied after different hormonal manipulations. orchiectomy reduced the uptake of 65zn. administration of estradiol benzoate to orchiectomized rats doubled the 65zn uptake, a phenomenon which was not observed in orchiectomized-adrenalectomized rats. adrenalectomy in orchiectomized rats had no effect on the concentration of radioactivity beyond the castration-induced decrease. a prolactin release inhibitor, 6-methyl-8-erogelenylacetamide, r ...197715955
pasteurella multocida bacteriophage adsorption.studied was the adsorption of 8 strains of pasteurella multocida bacteriophages presenting three groups of phages isolated in bulgaria. with the bacteriophages of groups i and ii the adsorption in broth in the first 10 minutes amounted to 50-65%, and at the 80 the minute reached its maximum value--98-99%. with the bacteriophages of group iii adsorption started with a lower percent and by the 140th minute reached 96-97%. it was by 60 min slower as compared with the adsorption with group i and ii ...197719872
[phlegmon of the fore arm succeeding to a dog-bite : respective role of the miscellaneous bacteria involved in this mixed infection (author's transl)].the authors report a case of a severe mixed infection (phlegmon of the fore arm) succeeding to a dog bite. a complex flora has been isolated from this phlegmom : pasteurella multocida, pasteurella pneumotropica, streptococcus mitis, actinomyces sp., bacteroides melaninogenicus, and a gram- bacteria related to group ii j. the pathogeny of such diseases is discussed, dwelling in one hand on the part of synergistic development of these various germs and in the other hand on the part played by saliv ...197934886
inactivation of pasteurella multocida within the mouse peritoneal cavity.normal icr mice were injected intraperitoneally with 0.5 ml of thioglycollate broth or 5 x 10(8) heat-killed pasteurella multocida vaccine and the number of polymorphs, lymphocytes and macrophages in the peritoneal washout suspensions were determined at intervals up to 72 hours. the stimulated mice were challenged intraperitoneally with opsonized or unopsonized p. multocida at increasing time intervals and the rate of growth by the organisms in the washout suspension was determined up to 60 minu ...197944602
human globin gene analysis for a patient with beta-o/delta beta-thalassemia.complementary dna (cdna) was prepared with rna-dependent dna polymerase from human globin messenger rna (mrna). annealing and translation experimenta with total mrna from circulating cells from a patient with heterozygous beta/heterozygous beta-delta-o thalassemia (beta-o/delta beta-o-thalassemia) demonstrated no detectable mrna for beta-globin. cdna enriched in sequences homologous to beta-globin mrna was prepared by hydroxylapatite fractionation of hybrids formed between beta-o/delta beta-o-th ...197549057
letter: joint infection by pasteurella multocida. 197549552
letter: joint infection by pasteurella multocida. 197551168
letter: joint infection by pasteurella multocida. 197551214
letter: joint infection by pasteurella multocida. 197551321
letter: joint infection by pasteurella multocida. 197553767
cell-mediated immune response in mice treated with steroidal contraceptives.the splenomegaly assay (simonsen, 1962) was standardized using different strains of rats and mice. male wistar rat (donor)-female swiss mouse (host) was found to be the suitable combination that could be employed in subsequent experiments to study the potential of contraceptive steroids to alter cmir. the index of splenomegaly appeared to increase in case of mice treated with combination oral contraceptives (ovulen, ovral or enovid). the differences observed, however, neared significance only in ...197555327
a serial study of pregnancy proteins in primigravidae.the plasma concentrations from four 'pregnancy proteins' and three steroid hormones have been measured throughout pregnancy in 15 primigravidae. two of the proteins, human placental lactogen (hpl) and pregnancy-specific beta1-glycoprotein (psbetag), are specific for pregnancy and correlate well with the stage of gestation. it is suggested that measurement of psbetag may be useful in assessing feto-placental wellbeing. neither of the pregnancy-associated proteins, steriod-binding beta-globulin (s ...197657799
protection of cattle against experimental haemorrhagic septicaemia by the capsular antigens of pasteurella multocida, types b and e.aluminum hydroxide adjuvant vaccines containing endotoxin-free capsular antigens of pasteurella multocida, types b and e, were administered to cattle. dose dependent serological responses were observed which were similar for both antigens. the immunised cattle were subjected to intravenous challenge by a virulent type e strain. all animals which received the highest vaccine dose survived and all unimmunised control animals died and a vaccine dose-response relationship was obtained. the results o ...197659379
pasteurella multocida infection: a cause of abdominal pain? 197769230
coagglutination test for identification of pasteurella multocida associated with hemorrhagic septicemia.two serogroups (b and e) of pasteurella multocida associated with hemorrhagic septicemia in cattle were differentiated by a coagglutination test with antibody-coated staphylococci. the group antigen was soluble and heat stable. it was not detectable in unencapsulated cells or their heated extracts. adsorbed onto human type o erythrocytes, it could be demonstrated by an indirect hemagglutination test with specific antiserum or by coagglutination with specific antibody-coated staphylococci. antibo ...197881214
serologic examination of the westphal-type lipopolysaccharides of pasteurella multocida.the serologic specificities of the westphal-type lipopolysaccharides from 16 serotypes of pasteurella multocida were compared with those reported for the heat-stable typing antigens in the gel diffusion precipitin test. like the heat-stable typing antigen, the westphal-type lipopolysaccharide from each of the serotypes reacted only with its homologous antiserum in 14 of the 16 serotypes. the lipopolysaccharide from type strains representing serotypes 2 and 5 reacted with either of the correspond ...197882415
dietary fibre and experimental colon cancer. 197985018
subcellular distribution and molecular heterogeneity of alpha 1-fetoprotein in newborn rat liver. 197986362
double-stranded protamine cdna: synthesis and characterization.double-stranded protamine complementary dna (cdna) was synthesized from a protamine mrna template via the single-stranded cdna intermediate using avian myeloblastosis virus reverse transcriptase. synthesis at 37 and 46 degrees c resulted in similar overall yields (greater than or equal to 18%), although the initial rate of synthesis was higher at 46 degrees c than at 37 degrees c. the dna of the second strand of the double-stranded cdna product was 84% resistant to prolonged digestion with exces ...197986381
spontaneous cytotoxicity of cultured human cell lines mediated by normal peripheral blood lymphocytes. ii. specificity for target antigens. 197986385
moloney virus-induced cell surface antigens and histocompatibility antigens are located on distinct molecules. 197987439
immune suppression in vivo with antigen-modified syngeneic cells. ii. t cell-mediated nonresponsiveness to fowl gamma-globulin.intravenous administration of syngeneic spleen cells coupled with the palmitoyl derivative of fowl gammma-globulin (p-f gamma g) results in a profound state of f gamma g-specific tolerance in c57bl/6 mice. administration of p-f gamma g coupled syngeneic cells specifically reduces both the primary and secondary hapten and carrier-specific pfc responses to tnp-f gamma g. since the haptenic response is affected, the tolerance functions at the level of the f gamma g-specific helper t cell. as few as ...197987447
the action of strontium on basophil leukocytes and its use to probe the relationship between immunologic stimulus and secretory response.strontium will substitute for calcium in the activation of histamine secretion from human basophil leukocytes stimulated by an immunologic reaction or by the ionophore a23187. strontium is required in 10-fold higher concentration (1 to 10 mm) to activate histamine release compared with calcium (0.1 to 1.0 mm). in terms of maximum release obtainable for a particular immunologic stimulus, strontium is more effective than calcium. results are presented to show that calcium and strontium act at the ...197987471
unusual translocations in ph1-positive acute lymphoblastic leukaemia. 197987666
[determination of antigen structures of pasteurella multocida: the preparation of vaccines].the discovery of the role played by somatic antigens of the pasteurella multocida a and d capsular types in the immunization of susceptible species makes it necessary to type the somatic antigens of the isolated strains prior to any action to induce active or passive immunity. only immunoprecipitation in gel was chosen out of the various typing techniques proposed, and a correlation was established between the capsular types (according to carter), somatic types (according to namioka) and types d ...197889055
studies on the action of histamine release by persulphates. 197989996
effects of arachidonic acid and its metabolites on antigen-induced histamine release from human basophils in vitro. 197990097
[neural secretion of histamine by the gastric mucosa and its effects on gastric motility]. 197890747
presence of plasma cells binding autologous antibody during an immune response.spleen and other lymphoid tissues of rabbits immunized with human serum albumin (hsa) and human lactoferrin (lf) were examined for the presence of cells forming anti-idiotype antibodies. to detect these cells, igg, f(ab')2, or fab' of specific antibodies were isolated, fluorochrome-tagged with tetramethylrhodamine isothiocyanate, and used as an idiotypic marker to detect splenic plasma cells that are producing anti-idiotypic antibody. by this procedure, we were able to demonstrate anti-idiotypic ...197991659
continuous production of monoclonal mouse ige antibodies with known allergenic specificity by cell fusion. 197991948
[deoxyribonucleic acids from pasteurella multocida and pasteurella haemolytica].pasteurella multocida and p. haemolytica strains contain between 1.5 and three per cent phosphorus, between nine and 14 per cent nitrogen, between two and four per cent dna, and between five and 18 per cent rna, the precise figures depending on culturing conditions. high-molecular dna may be isolated by means of bacteriolysis, using deoxycholate or dodecylsulphate and the usual steps of purification, with yield and purity differing by strains. dna with sufficient purity can be obtained from seph ...197993955
chelation in metal intoxication. vi. influence of pas and cdta on the excretion of manganese in rabbits given mno2.the success of p-aminosalicyclic acid (pas) and 1,2-cyclohexylene dinitrilotetraacetic acid (cdta) in the mobilization of manganese from some vital organs and their subcellular fractions of manganese-intoxicated rats, led to the two compounds beng further investigated individually and in combination for their efficacy in influencing excretion of manganese in rabbits given mno2. these chelating agents led to a higher excretion of manganese through faeces than urine. whereas cdta was able to enhan ...197896549
[intestinal rest. technique. indications. results (author's transl)]. 197896646
[transplantation of the big toe from the foot to the hand in monkeys using a microsurgical technic]. 197897465
effects of pelleting and sterilization of diet on 2 strains of rabbit coccidia.both autoclaving and irradiation at levels currently used for other laboratory animal diets appear to be satisfactory for the elimination of 2 of the most robust and pathogenic species of rabbit coccidia. pelleting by the usual methods, even at 68 degrees c, was found to be unsatisfactory in eliminating all oocysts from heavily infected diet.197897471
[sarcocyst resistance to certain factors]. 197897852
in vitro antibacterial activity of amikacin and ticarcillin, alone and in combination, against pseudomonas aerurginosa.in vitro antimicrobial susceptibility studies using amikacin and ticarcillin, alone and in combination, were performed on 20 strains of pseudomonas aeruginosa. all strains were susceptible to amikacin, and ticarcillin was active against 16 of the 20 strains. enhanced anti-pseudomonas activity could be demonstrated with the combination of amikacin and ticarcillin.197898109
carrier rate of pasteurella multocida in a cattle herd associated with an outbreak of haemorrhagic septicaemia in the sudan. 197898205
synergism between morphogenetic mutants of myxococcus xanthus. 197898366
immunopathology of malaria. 197898476
pleiotropic effect of a rifampin-resistant mutation in bacillus subtilis.rifampin-resistant (rifr) mutants were isolated spontaneously from bacillus subtilis strain 168. a fraction of the mutants did not grow on a minimal medium. a high concentration of one of the l-amino acids (glutamic acid, glutamine, arginine, proline, aspartic acid, or asparagine) was required to restore their growth on the medium. further analysis of one of the mutants (strain rf 161) suggested that the mutant is unable to use ammonia as a nitrogen source and requires amino acids instead. activ ...197898517
evidence that the regulation of diphtheria toxin production is directed at the level of transcription.it has been known for several decades that iron inhibits the production of diphtheria toxin by corynebacterium diphtheriae by preventing expression at maximal levels. we examined the inhibition kinetics of toxin production after the addition of either iron or rifampin to iron-limited cultures of c7 (betatox+). iron-mediated inhibition of toxin production was found to be linear within the range of 16 nm to 16 micron. the inhibition kinetics following the addition of iron or rifampin was almost id ...197898519
jaundice and breast-feeding among alaskan eskimo newborns.the course, incidence, and severity of neonatal jaundice was studied in 95 alaskan eskimo infants. breast-fed infants had higher bilirubin concentrations than bottle-fed babies. both groups experienced high bilirubin levels, similar to those previously reported in navajo and oriental infants but greater than those observed in whites and blacks. a marked capacity to inhibit hepatic glucuronyl transferase was observed in breast-milk specimens but only partly accounted for the bilirubin differences ...197899026
[transplantation of langerhans' islands]. 197899549
development of leucocytozoon caulleryi in chick embryos infected by biting of culicoides arakawae through shell membrane.chick embryos were infected with leucocytozoon caulleryi by biting of the midge, culicoides arakawae, through the shell membrane. schizonts of l. caulleryi were detected in the chorioallantoic membrane and most of the internal organs of embryos and of chicks after hatching. the development of schizonts was slower in embryos than in chickens. soluble antigens of l. caulleryi were demonstrated by the precipitation test in the allantoic fluid and blood from the embryos and chicks. no erythrocytic s ...197899671
[sensitivity of bordetella bronchiseptica, pasteurella multocida and mycoplasma hyorhinis strains isolated from pigs towards antibiotics and chemotherapeutic agents]. 1978100928
transcription and translation in a pleiotropic streptomycin-resistant mutant of escherichia coli.the role of the ribosomal protein s12 (streptomycin protein) in ribosome function and in other metabolic processes in the cell has been investigated. a spontaneous streptomycin-resistant strain of escherichia coli (sm3) carrying a mutation in the rpsl gene is deficient in its ability to induce the synthesis of the enzyme bets-galactosidase. it was demonstrated that the reduced rate of enzyme synthesis results from deficiencies in both the transcription of the lactose operon and translation of th ...1979104958
[the role of the culture medium in the sensitivity of gram-negative stocks and their resistant mutants to fosfomycin. the influence of glucose-6-phosphate]. 1978105326
[evaluation of the immune state of poultry against newcastle virus via various serological reactions].studied were 336 sera of vaccinated and challenged chickens, 76 egg extracts, and 36 birds following challenge for the presence of serum antibodies against the newcastle disease virus, using the hemagglutination-inhibition reaction, the agar gel diffusion precipitation reaction, and the virus-neutralizing test. the values of the antihemagglutinins, precipitins, and virus-neutralizing antibodies were investigated in relation to the immunobiologic resistance of birds to a velogenic newcastle disea ...1978105460
antigenic relationship between influenza c viruses.the object of this study was to determine whether antigenic groupings exist among influenza c viruses. altogether seven influenza type c strains were examined by reciprocal haemagglutination-inhibition and serum neutralization tests with sera produced in ferrets. some biological properties were also compared and differences were found in their ability to agglutinate erythrocytes from various species, in their ability to adsorb onto rat and fowl erythrocytes, and in their rates of elution from th ...1978104696
heterologous radioimmunoassay for rabbit prolactin.a highly specific heterologous double-antibody ria has been developed to measure rabbit prl by using guinea pig antiserum to human prl and ovine [125 i]iodo-prl. rabbit pituitary prl and serum give parallel dose-response curves in the assay and no cross-reaction (less than 0.1%) occurs with gh, placental lactogens, lh, fsh, or tsh from several different species. the assay is suitable for the measurement of human, ovine, bovine, caprine, and canine prl in addition to rabbit prl, but shows no cros ...1978105878
the metabolism and disposition of 3,4,4'-trichlorocarbanilide in the intact and bile duct-cannulated adult and in the newborn rhesus monkey (m. mulatta). 1978106490
blood-brain barrier opening by isotonic saline infusion in normotensive and hypertensive animals.the blood-brain barrier to intravascular evans blue-albumin was opened in monkeys and rabbits by infusing isotonic saline for 15 s into the common carotid artery, when the external carotid was clamped temporarily and the lingual was catheterized for measuring pressure. barrier opening correlated better with infusion pressure than with infusion rate, and occurred at carotid artery pressures above 170 mmhg. systemic hypertension induced by aramine increased barrier vulnerability by causing a highe ...1978104549
a comparison of tissue gold levels in guinea-pigs after treatment with myocrisin injected intramuscularly and triethylphosphine gold chloride and myocrisin administered orally.a comparative study of tissue gold levels produced in guinea-pigs after the oral administration of either triethylphosphine gold chloride or myocrisin (sodium aurothiomalate) or after the injection of myocrisin intramuscularly is reported. gold concentrations were determined 5, 24 and 168 hours after administration in stomach, small intestine, large intestine, kidney, liver and spleen and 5 and 24 hours after administration in skin, adrenals, heart, lung and brain. in gastrointestinal tissues, t ...1978102133
fascioloides magna: development in selected nonruminant mammalian hosts. 1979108129
sexual behavior and hormone levels during the menstrual cycles of rhesus monkeys. 1978108191
bacterial fusion assayed by a prophage complementation test.in previous studies of bacterial protoplast fusion, only the frequencies of cell wall regeneration and of bacterial recombination were determined. in this work the frequency of the heterozygous fusion products is measured by prophage complementation. two multiply marked nonsuppressing strains of bacillus subtilis, each lysogenic for a different sus mutant of the phage phi 105, were induced by mitomycin c, protoplasted, fused, and, after dilution in hypertonic broth, incubated until plating with ...1979108245
medical and surgical approach to laryngeal air sacculitis in a baboon caused by pasteurella multocida.air sacculitis was diagnosed in a chronically chaired baboon, papio anubis. pasteurella multocida was repeatedly isolated from the air sac for a period of 1 year. the condition was characterized by the continuous accumulation of either mucoserous or purulent fluid. biopsies of the air sac taken during the course of the disease initially revealed goblet cell hyperplasia. later a subepithalial mononuclear or polymorphonuclear infiltrate and necrosis was the predominate finding. medical therapy was ...1978108467
the effects of alcohol on agonistic behavior in the telomian dog.the study analyzed the effects of alcohol on agonistic behavior in three independent social systems, each compromised of two male and one female telomian dog. three dose levels were used: 0, 0.8, and 1.6 g/kg body weight of absolute alcohol diluted to 20%. observations were made when none, one (top, middle or low ranking), or all dogs in a system received a given treatment. at the 0.8 g/kg level, low ranking dogs showed an increased frequency of attacks and bone possession time, while top and mi ...1979108707
mao inhibition and the effects of centrally administered lsd, serotonin, and 5-methoxytryptamine on the conditioned avoidance response in rats.pretreatment with the mao-inhibitors iproniazid, clorgyline, or deprenyl abolishes the effects of lsd on the conditioned avoidance response (car) in rats. the effects of serotonin (5-ht) and 5-methoxytryptamine (5-mt) are greatly potentiated by these substances. brain levels of lsd are not affected by mao inhibition whereas levels of 5-ht and 5-mt are significantly elevated. it is postulated that the decreased behavioral response to lsd is the result of 'mao inhibitor-induced' changes whereas th ...1979108709
blood groups of macaques:a comparative study.distribution of the human-type and of the simian-type blood groups in rhesus, crab-eating, bonnet, pig-tailed and stump-tailed macaques revealed significant similarities and differences among these species. human-type a--b-o blood groups cut across taxonomic lines and seem less value for taxonomic purposes than the simian-type blood groups detected by cross-reacting isoimmune rhesus monkey sera.1978101667
biochemical and serological studies on pasteurella multocida isolated from cattle in the sudan.of 42 strains of pasteurella meltocida isolated from different outbreaks of hemorrhagic septicaemia and from healthy cattle in various parts of the suden, 38 belonged to carters' type b and four to type e. the strain used for vaccine production was type e. with respect to somatic antigen determination, there was some correlation between the results of the gel diffusion and the agglutination tests as most strains reacted with 0 groups 6 antisera in both tests. however, some antigens cross-reacted ...1979110516
in vivo regulation of glycolysis and characterization of sugar: phosphotransferase systems in streptococcus lactis.two novel procedures have been used to regulate, in vivo, the formation of phosphoenolpyruvate (pep) from glycolysis in streptococcus lactis ml3. in the first procedure, glucose metabolism was specifically inhibited by p-chloromercuribenzoate. autoradiographic and enzymatic analyses showed that the cells contained glucose 6-phosphate, fructose 6-phosphate, fructose-1,6-diphosphate, and triose phosphates. dithiothreitol reversed the p-chloromercuribenzoate inhibition, and these intermediates were ...1978101523
[bactericidal effect of disinfectants on various strains of mycobacterium tuberculosis]. 1979113848
double antibody specificity of lymphoid cells isolated from normal human intestinal mucosa.lymphoid cells were isolated from normal human mucosal specimens. a certain fraction of these cells exhibited double antibody specificities at the levels of both light kappa and lambda chains and heavy mu and gamma chains. the variable frequency with which these cells were detected appears to be a characteristic of this population. they represent a very low proportion of the total number of cells isolated.1979114243
a macrophage chemotactic factor sharing common antigenicity with immunoglobulin g from dnp-ascaris extract-induced skin lesion in guinea-pig.a macrophage chemotactic factor (factor a) was extracted from dnp-ascaris extract-induced skin lesions (showing a maximal macrophage reaction) in guinea-pigs and then purified by gel filtration on sephadex g-100 and chromatography using deae-sephadex and cm-sephadex. this material was a thermolabile protein (free of nucleic acid) with a molecular weight of about 150,000. it shared commom antigenicity with serum igg, and its chemotactic acitivity was completely absorbed by anti-igg and anti-light ...197898903
experimental spinal cord trauma, ii: blood flow, tissue oxygen, evoked potentials in both paretic and plegic monkeys.graded clinical motor deficits were produced in a series of rhesus monkeys subjected to experimental spinal cord trauma from a variety of impact loads. an argon washout technique was used to measure spinal cord blood flow; tissue oxygen carbon dioxide, and somatosensory evoked potentials (seps) were also monitored. each animal received a clinical grade and, after a week of study, the spinal cords were removed for histopathological grading. blood flow in paraplegic animals was significantly decre ...197898856
in vitro response to rosette inhibition factor (rif) and rosette restoring factor (rrf) of human lymphocyte after fractionation on a discontinuous density albumin gradient.in vitro studies on peripheral blood lymphocytes from tuberculous patients and healthy subjects are reported. factors isolated from human serum used in the experiments included the rosette inhibition factor (rif) from serum of tuberculous patients and the rosette restoring factor (rrf) from serum of healthy subjects. lymphocytes were fractionated on a discontinuous bsa gradient. lymphocytes from healthy subjects, sensitive to rif, as well as lymphocytes from tuberculous patients, sensitive to rr ...1979117785
comparison of pasteurella multocida serotyping systems.thirty-two type specific cultures used in four typing systems for serologically classifying pasteurella multocida were compared as originally described for: (1) little and lyon's plate agglutination test; (2) carter's indirect hemagglutination test, hyaluronidase decapsulation test, and acriflavine reaction; (3) namioka's plate and tube agglutination tests; and (4) heddleston's gel diffusion precipitin test. in addition, seven cultures from robert's five passive protection groups were included. ...1979118694
serotyping of pasteurella multocida isolants from poultry.a total of 607 pasteurella multocida strains were isolated in israel from turkeys, geese, and chickens. serotyping was carried out by the gel-diffusion precipitin test. the antigens were heat-stable saline extracts of the organisms, and the diagnostic sera were produced in chickens using formalinized bacterins. among 31 serotypes containing one to five antigenic factors, serotypes 1 and 3 accounted for 58.1%, 80.8%, and 88.9% isolants from turkeys, geese, and chickens, respectively. on the basis ...1979118742
thyroid-stimulating hormone: neuroregulation and clinical applications. part 2. 197898254
studies of immunogenicity of westphal lipopolysaccharides from pasteurella multocida in mice. 1979120378
[decarboxylase activity study of pasteurella multocida].ornithine-, lysine- and arginine-decarboxylase activity of 218 p. multocida strains, isolated from birds of varying disease symptoms in bulgaria and cssr, and from pigs, rabbits and birds in cuba, ussr and cssr, was studied after the method of moller. positive ornithine decarboxylase activity was established in 211 strains, low -- in 2, and negative -- in 5 strains. low arginine decarboxylase activity was observed in 12 pasteurella strains, while in 14 -- low lysine decarboxylase activity. the p ...1979120631
[personal prophylaxis of venereal diseases]. 197897875
fatal immune complex glomerulonephritis without deposits.repeated intravenous injections of egg albumin in rabbits produced small antigen-excess complexes and severe glomerulonephritis. immunoglobulins and complement in the glomeruli were not clearly demonstrated by immunofluorescence; deposits were found to be sparse by electron microscopy. this study demonstrates that soluble immune complexes are responsible for the glomerular reaction. the apparent absence of deposits is thus not sufficient to exclude an immune complex pathogenesis.1975123971
[possible causes determining the developmental rhythm of populations of the nasopharyngeal gadfly of sheep, oestrus ovis l. (diptera, oestridae)].on the basis of observations undertaken in azerbaijan and available literary data the author considers the hypotheses concerning the reasons causing the delay of the ist instar larvae development and determining the age structure changes in population of oestrus ovis l. within a year. the main reasons are as follows: factors depending on the population density at the larval phase, the light day duration within the life cycle of the botfly females.1975124845
morphogenesis of the membrane-bound electron-transport system in sporulating bacillus megaterium km.the properties of electron transport systems present in soluble and particulate fractions of spores of bacillus megaterium km?have been compared with those of similar fractions prepared from exponential-phase vegetative cells of this organism. the timing and localization of modifications of the electron transport system occurring during sporulation have been investigated by using a system for separating forespores from mother cells at all stages during development [8]. spore membranes contained ...1975126154
[phenotypic expression of colon polymorphic antigens (wz) in human colon adenocarcinomas].human colon adenocarcinomas from 52 patients were investigated for the presence of the colon polymorphic antigens wz. the patients were typed for their wz phenotype, using the immunofluorescence method on non tumoral colon mucosa sections: 27 patients were found w+ z+, 18 w- z+, and 7 w- z-. the tumors were tested for the presence of the wz phenotypes, using the immunofluorescence method and a radio-immunoassay. the wz phenotypes were not expressed in the non secreting tumors, whatever the patie ...197897030
cytogenetics and the pathologist. 1975126409
solubilization and partial purification of prostaglandin endoperoxide synthetase of rabbit kidney medulla.the microsomes of rabbit kidney medulla converted arachidonic acid into prostaglandin e2 in the presence of hemoglobin, tryptophan and glutathione as activators. when themicrosomal suspension was treated with 1% tween 20, a solubilized enzyme was obtained which catalyzed the conversion of arachidonic acid to prostaglandins g2 and h2. the solubilized enzyme was adsorbed to and then eluted from an omega-aminooctyl sepharose 4b column, resulting in about 10-fold purification over the microsomes. th ...197896862
synthetic antigens iii. antigen-binding cells to interpolymer of styrene and maleic acid psm and sheep red blood cells in mice immunized with psm.studies on the immune response in mice to a new synthetic antigen interpolymer of styrene and maleic acid (psm) are described. the immune response was measured by determination of antigen binding cells by means of rosette test with psm-coated srbc, an unexpected effect was observed--besides rosette-forming cells for psm, rosette-forming cells and agglutinating antibodies for sheep red cells were found. the results presented in this paper suggest that psm is acting both as an immunogen and a poly ...197994989
[histological and histochemical studies of the gastric mucosa of the mouse under normal conditions and following various treatments]. 197994241
effect of essential phospholipids on the properties of atpases of isolated rat liver plasma membranes of young and old animals."essential" phospholipids (epl), rich in dilinoleyl-phosphatidylcholine were given orally to young and 780-800 day-old rats. the double-labelled (14c/32p) dilinoleyl-phosphatidylcholine was incorporated in plasma membranes and the amounts incorporated into different organs investigated. old animals incorporated higher radioactivity than young. the 14c/32p ratio however decreased in comparison with the substance administered. the amount of (na+-k+)-atpase in rat liver plasma membranes of old anim ...1975127894
evidence for reappearance of trypanosoma brucei variable antigen types in relapse populations.seven out of 11 bovines infected with different clones of trypanosoma brucei showed 2 peaks of antibody activity against the infecting clone within 7 weeks, as measured by immunofluorescence, radioimmunoassay, and neutralization of infectivity tests. using other clones from an unrelated stock, antibodies to these clones were not detectable, indicating that the antibodies produced were specific to the infecting organisms. these results suggest that there was a reappearance or increase in numbers ...197992560
using the hml1 strain of histomonas meleagridis in evaluating chemotherapeutants in chicks.ten-day-old white leghorn cockerels were inoculated per cloaca with 500,000 culture forms of the hml1 strain of histomonas meleagridis. birds dying on the 6th and 7th days and birds necropsied 7 days postinoculation had prominent cecal and liver lesions indicative of severe histomoniasis. dose responses were obtained with three 1,2-disubstituted 5-nitroimidazoles blended into the feed at various concentrations, demonstrating the utility of this virulent strain in chemotherapy studies.1975128345
microflora of pneumonic lungs in a pig herd established by hysterectomy.mycoplasmas and other micro-organisms are recorded from a pig herd originally derived by hysterectomy. complement fixation tests were positive for mycoplasma hyopneumoniae 20 months after the first hysterectomy. following a marked increase in pneumonic lesions found at slaughter, and after modifying the culture medium m hyopneumoniae was isolated. the principal micro-organism associated with this mycoplasma was pasteurella multocida.1976130669
immunofluorescent staining of platelet suspensions and detection of antiplatelet antibody in patients with idiopathic thrombocytopenic purpura.direct immunofluorescent staining of 31 specimens of platelets obtained from 13 cases of idiopathic thrombocytopenic purpura (itp) revealed positive staining on the surface of platelets for both immunoglobulins (igs) and human b1c globulin in 9 specimens, for only igs in 1 specimen and for human b1c alone in 5 specimens. the pattern of the positive immunofluorescent staining was granular. indirect immunofluorescent staining of normal platelets in serum obtained from patients with itp was positiv ...197988810
a silver impregnation method for reactive microglia in 1 mum epoxy sections.a simple rapid ammoniacal silver nitrate impregnation method for reactive microglia in 1 mum epoxy-embedded tissue is presented. this has been applied to demonstrate argyrophilic microglia in stab-wounded rat brain and in the human cerebellar cortex.197988157
immune mechanism in pasteurella multocida-infected mice.various immunizing procedures were tested for their effect on the growth of pasteurella multocida in specific pathogen-free cd-1 mice. two injections with killed vaccine incorporated into freund complete adjuvant gave excellent protection against parenteral challenge, but less to control the growth of an aerogenic inoculum. protection with adjuvant-treated preparation was always superior to that seen with non-adjuvant-treated vaccines. a killed saline-suspended vaccine introduced aerogenically m ...1976131783
[spontaneous peripheral proteolysis/1st communication: method and results of clinical examinations (author's transl)].with a modificated astrup-fibrin plate-method also an inhibition of proteolysis can be registrated. in various medical areas a spontaneous peripheral proteolysis had been found, especially so in chronical bacterial infections.1976132940
allergic reaction to allopurinol with cross-reactivity to oxypurinol.a 25-year-old white man with gout and nephropathy and with a previous reaction to allopurinol was given a trial dose of oxypurinol. he developed malaise, a generalized erythematous reaction with edema, pruritus, and emesis; this was clinically identical to the reaction he experienced with allopurinol. when the patient's lymphocytes were exposed in vitro to oxypurinol and allopurinol, increased dna synthesis was observed, suggesting an immunologic basis for the reaction. this patient indicates th ...1976134655
dynamics of sulfonylurea-induced insulin release from the isolated perfused rat pancreas.in the isolated perfused rat pancreas various sulfonylurea drugs were tested with a basal glucose level of 1 mg/ml in the perfusion buffer and were found to cause a biphasic insulin response. novocs 476, a new and potent sulfonylurea, and glibenclamide qualitatively differed from tolbutamide, glibornuride, glipizide, and glisoxepide, which were all alike in terms of the relationship between first and second phases of insulin release.1976134920
immunochemical properties of embryonic and adult specific antigens of chicken erythrocytes. 197986451
[resistence of head lice against gammexane (lindane) applies also to the nonalcoholic preparations]. 197985264
differential mechanisms of increased alpha 1-fetoprotein production in rats following carbon tetrachloride injury and partial hepatectomy.possible differences in the mechanisms of increased alpha1-fetoprotein (afp) production following carbon tetrachloride (ccl4) intoxication and partial hepatectomy were studied with 5-week-old rats at the time of sacrifice. the maximum level of serum afp reached in 4 days after a single dose of ccl4 was much higher than that after partial hepatectomy, although the incorporation of [3h]thymidine into liver dna increased nearly to the same extent by either of these treatments. in the remnant after ...197658715
selective and non-selective lymphocytotoxicity in human melanoma: observation on the effect of long-term culture and fetal bovine serum on target-cell sensitivity to lymphocytes.in vitro cell mediated cytotoxicity (cmc) assays have been conducted in a human melanoma system with a 3h-proline retention technique. melanoma target cells from long-term cultures ("cell lines") are found to exhibit increased susceptibility for lymphocyte cytotoxicity in comparison to the same target cells from short-term culture. the higher sensitivity of the "cell line" derived target cells is seen with lymphocytes, irrespective of diagnosis of the donor. in parallel experiments with the targ ...197553212
preparation of human immunoglobulin free of plasmin and anticomplement activities.human igg separated by cohn fractionation showed variability in the content of aggregates, plasminogen and anticomplement activity. the plasminogen was removed or markedly reduced by affinity chromatography on sepharose-lysine. anticomplement activity was reduced by chromatography of cohn fraction ii on deae-cellulose. preparations of igg obtained by chromatography of intermediates from cohn fractionation (e.g. cohn fii + fiii or fii + fiii w) on deae-cellulose were devoid of aggregates, plasmin ...1977140530
[cardiac arrhythmia in the rabbit under the effect of adrenaline and difluorodichloromethane (fc12)].inhalation of gas mixtures containing different concentrations of fc12 by anesthetized and normally oxygenated rabbits produces blood levels of fc12 which are stable and proportional to the rate of fc12 in the mixture. from the arterial concentration of 80 microgram/ml fc12 (10 % fc12) mixture) and over, fc12 alone causes effects proportional to doses: arterial pressure decrease with tachycardia; slight morphological alterations of the electrocardiogram at high concentration. arrhythmia never oc ...1977145298
laparoscopic examination of the reproductive tracts of gilts using a special crate.a crate for restraining gilts for routine laparoscopic examination of the reproductive tract is described, together with the techniques employed in anaesthesia and laparoscopy. the procedure is efficient, allowing two operators to examine 4-5 gilts per hour.1978148883
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