| cultural and biochemical criteria for the identification of haemophilus spp from swine. | twenty-two haemophilus cultures of types prevalent in swine and of different geographic origins were subjected to biochemical and cultural examinations. three subgroups were identified: one was unrease-positive, produced porphyrin from delta-aminolevulinic acid, and grew on infusion mediums supplemented only with v factor; the 2nd was unrease-negative, porphyrin-positive, and grew only on serum-enriched mediums with added v factor; and the 3rd was unrease-negative, porphyrin-negative, and grew o ... | 1977 | 189648 |
| haemophilus parahaemolyticus serotypes. serological response. | serotypes 1,2,4 and 5 of haemophilus parahaemolyticus were inoculated into, respectively, 4,4,2 and 5 pigs. serum samples were tested for circulating antibodies by the modified complement fixation test (cf test). when individual serotypes were used as antigen, titers were found only to the serotype which had been used for inoculation. using antigen in which the serotypes were pooled, antibodies were demonstrated in sera from all the pigs. the cf titers obtained with the pooled antigen were equiv ... | 1979 | 523310 |
| haemophilus parasuis infection in swine. | septicemic disease occurred in 49 of 126 pigs several days after being transported 80 km. all affected pigs died. the main changes in acutely affected pigs were skin discoloration, pulmonary edema, arthritis, meningitis, and renal glomerular thrombosis. in peracute cases, gross findings were minimal. haemophilus parasuis was isolated from multiple organ sites in most affected pigs. haemophilus parasuis was isolated from nasal swab specimens from 17 of 20 clinically normal pigs on the farm of ori ... | 1977 | 914694 |
| fimbriae and membranes on haemophilus parasuis. | in the electron microscope an additional layer (glycocalix) of the cell wall and fimbriae on haemophilus parasuis were shown in thin sections of the infected cam which have their origin on the cm of the haemophilus parasuis-cells. no fimbriation was seen after conventional cultivation. | 1992 | 1349784 |
| effect of oxygen supply during growth on the production of cytochromes, enzymes, and acid end products by haemophilus parasuis. | haemophilus parasuis, grown under conditions of high aeration, was found to lack a tricarboxylic acid cycle but to possess phosphoenolpyruvate carboxylase and a reductive pathway leading to the production of succinate. such organisms contained approximately equal quantities of b-, c-, and d-type cytochromes and excreted acetate. when the oxygen supply for growth was either reduced or eliminated, the specific activities of phosphoenolpyruvate carboxylase, malate dehydrogenase, fumarase, fumarate ... | 1992 | 1464068 |
| [occurrence and drug resistance of bacteria pathogenic to the lungs from autopsy material of swine]. | eight hundred and fifty-four piglets which died or were euthanized due to pneumonia or rhinitis atrophicans, were investigated during the period of 1986-1990. of the animals, 569 showed bronchopneumonia, 218 had pleuritis, pericarditis and peritonitis, 165 had rhinitis atrophicans, 58 pleuropneumonia, and 9 animals had fibrinous pneumonia. pasteurella multocida, haemophilus parasuis, bordetella bronchiseptica, actinobacillus pleuropneumoniae and pasteurella haemolytica were isolated in 59.1%, 29 ... | 1992 | 1481214 |
| prevalence of haemophilus parasuis serovars among isolates from swine. | two hundred sixty haemophilus spp isolates that had been obtained from the respiratory tract and other sites of swine were acquired from diagnostic laboratories, primarily in the united states and canada. the majority of isolates (243/260) were biochemically characterized as h parasuis; however, a few isolates of taxa distinct from h parasuis (taxa "minor group," d, e, and f) were identified. fourteen h parasuis serovars were identified, and of those previously described, the most prevalent were ... | 1992 | 1524289 |
| designation of 15 serovars of haemophilus parasuis on the basis of immunodiffusion using heat-stable antigen extracts. | previous independent investigations of the serotyping of haemophilus parasuis strains have led to the designation of serovars a to d, 1 to 7, jena 6 to jena 12, and nd1 to nd5. heat-stable antigen preparations from the reference strains for these serovars were tested by immunodiffusion with rabbit hyperimmune antisera. the existence of 15 distinct serologic groups was apparent, for which we propose the designations serovars 1 to 15. examination of 290 field isolates from swine in the former germ ... | 1992 | 1572971 |
| comparative virulence of haemophilus parasuis serovars 1 to 7 in guinea pigs. | reference strains for haemophilus parasuis serovars 1 to 7 were examined for virulence by inoculation of guinea pigs. guinea pig response to intraperitoneal inoculation was similar for the 7 reference strains. however, apparent differences in virulence were detected after intratracheal inoculation. cells of the references strains for serovars 1 and 5 were most invasive, causing moribundity or death at higher doses and a persistent septicemia at lower doses. haemophilus parasuis could be isolated ... | 1992 | 1626790 |
| vaccination of gnotobiotic primary specific pathogen-free pigs against haemophilus parasuis. | three trials were conducted to establish if young primary specific pathogen free (spf) pigs could be protected from glasser's disease by vaccination. three age groups of cesarean-derived isolator-reared gnotobiotic pigs were vaccinated twice at 4 and 6, 3 and 5, and 2 and 4 wk of age respectively with a formalin killed aluminum hydroxide adsorbed bacterin prepared from three strains of haemophilus parasuis isolated from ontario pigs affected with glasser's disease. when challenged two weeks late ... | 1991 | 1832078 |
| cross protection among haemophilus parasuis strains in immunized gnotobiotic pigs. | in an attempt to establish if cross protection can be induced by different strains of haemophilus parasuis, three groups of 12 gnotobiotic pigs were immunized each with an aluminum hydroxide adsorbed whole cell bacterin of one of three h. parasuis strains. two weeks later, four pigs within each vaccinated group were challenged with aerosols of live cultures of each of the three test strains and observed for response. two virulent strains v1 and v2 protected all the vaccinated pigs, while all non ... | 1991 | 1884282 |
| the cytotoxin of actinobacillus pleuropneumoniae (pleurotoxin) is distinct from the haemolysin and is associated with a 120 kda polypeptide. | mutants of actinobacillus pleuropneumoniae strain hk 361 (serotype 2) were isolated which were deficient in type ii (ca2(+)-dependent) haemolysin activity (hly-). some of the hly- mutants secreted a potent, heat-labile extracellular cytotoxic activity against porcine alveolar macrophages. comparison of cell-free culture supernatant from the parent strain and some hly- mutants by sds-page and immunoblotting revealed the loss of a major extracellular polypeptide of 109 kda. two hly- mutants which ... | 1991 | 2033378 |
| v factor-dependent members of the family pasteurellaceae in the porcine upper respiratory tract. | a study was performed to obtain a better understanding of the diversity and ecology of members of the family pasteurellaceae in the porcine respiratory tract. a collection of 132 v factor-dependent strains of pasteurellaceae selected from porcine field isolates mainly from the respiratory tract were subjected to detailed characterization. in addition to the three hitherto recognized species actinobacillus pleuropneumoniae, haemophilus parasuis, and haemophilus taxon "minor group," three distinct ... | 1990 | 2280002 |
| in vitro susceptibility of selected veterinary bacterial pathogens to ciprofloxacin, enrofloxacin and norfloxacin. | the minimum inhibitory concentrations (mic) of ciprofloxacin, enrofloxacin, and norfloxacin were tested for approximately ten clinical isolates of each of actinobacillus pleuropneumoniae, actinobacillus suis, actinomyces pyogenes, corynebacterium pseudotuberculosis, erysipelothrix rhusiopathiae, haemophilus parasuis, haemophilus somnus, pasteurella haemolytica, pasteurella multocida, rhodococcus equi, streptococcus equi, streptococcus suis and streptococcus zooepidemicus. ciprofloxacin and enrof ... | 1990 | 2306672 |
| preliminary assessment of a haemophilus parasuis bacterin for use in specific pathogen free swine. | a whole cell formalin killed trivalent haemophilus parasuis bacterin was tested for efficacy in four week old, weaned specific pathogen free pigs challenged under laboratory conditions. the vaccine contained three field strains of h. parasuis selected from confirmed cases of glasser's disease. two different formulations were evaluated in separate trials. in trial 1, ten pigs received 5 ml of bacterin subcutaneously in the neck, followed by a second 5 ml dose two weeks later. another ten pigs ser ... | 1989 | 2531627 |
| characterisation and antimicrobial sensitivity of haemophili isolated from pigs. | a total of 70 haemophili from australia pigs was compared with a range of reference strains of porcine haemophili. forty-eight of the isolates were identified as actinobacillus pleuropneumoniae biovar 1 and the remaining 22 isolates as haemophilus parasuis. forty one of the a. pleuropneumoniae isolates were used in a study to determine to the minimal inhibitory concentration (mic) of 12 antimicrobial agents, or combinations of agents. penicillin, neomycin, trimethoprim, trimethoprim-sulphamethox ... | 1989 | 2930386 |
| defining the metabolic and growth responses of porcine haemophili to exogenous pyridine nucleotides and precursors. | a variety of biologically important pyridine nucleotides and precursors were examined for their capacities to satisfy the v-factor requirement of 30 strains of porcine haemophili. of the compounds tested, only nad, nmn and nicotinamide riboside (nr) supported the growth of all strains; nadp supported the growth of only the type strain of haemophilus parasuis. further studies with the h. parasuis type strain and the neotype strain of h. pleuropneumoniae demonstrated that, during growth, these org ... | 1986 | 2942635 |
| pyridine nucleotide metabolism by extracts derived from haemophilus parasuis and h. pleuropneumoniae. | a variety of biologically important pyridine nucleotides and precursors were examined for their capacities to serve as substrates for the synthesis of nad by cell fractions derived from haemophilus parasuis and h. pleuropneumoniae. of the compounds tested, only nmn and nicotinamide riboside were converted to nad. these reactions required atp as co-substrate, and fractions from both organisms could also catalyze the atp-dependent synthesis of nadp from nad. in the absence of atp, and depending on ... | 1986 | 2946387 |
| some antigenic properties of haemophilus parasuis and a proposal for serological classification. | we propose a serological classification of haemophilus parasuis into at least five serovars, using an agar-gel-precipitation test with extracts from autoclaved cells. thirty-two strains were examined, and it was possible to classify 26 of them. the specific antigens were thermostable and soluble and were not affected by pronase treatment but could be extracted by phenol, suggesting a polysaccharide. this polysaccharide seemed to be identical with the capsular substance of serovars 1, 2, and 3. i ... | 1986 | 3086374 |
| tryptone-yeast extract broth as a culture medium for haemophilus pleuropneumoniae and haemophilus parasuis to be used as challenge inocula. | haemophilus pleuropneumoniae and haemophilus parasuis were grown in a liquid medium containing tryptone and yeast extract. whereas culture turbidity during exponential growth of either organism was proportional to the number of viable cells, continued incubation of stationary phase cultures was accompanied by a dramatic decrease in viability with no corresponding decrease in culture turbidity. it is concluded that the medium described should prove useful for the growth of porcine haemophili for ... | 1986 | 3755642 |
| comparative virulence of porcine haemophilus bacteria. | the virulence of strains of haemophilus pleuropneumoniae serotype 1, 2, 3, 7 and strains of the "minor-group" and haemophilus parasuis were compared by inoculating specific pathogen-free pigs into the lower airways with specified doses of bacteria. haemophilus pleuropneumoniae, strain w, serotype 1, given in 1 x 10(8) colony-forming units, produced a lethal acute pleuropneumonia in four pigs. nonlethal localized pulmonary necrosis was induced in four groups of two pigs given 1 x 10(7), 1 x 10(6) ... | 1985 | 3986682 |
| [serological typing of haemophilus parasuis]. | | 1985 | 4096629 |
| [haemophilus parasuis infection in swine]. | | 1974 | 4825345 |
| dilution technique for isolation of haemophilus from swine lungs collected at slaughter. | a total of 307 lungs obtained from a slaughterhouse were cultured by a dilution technique for the isolation of haemophilus spp. the technique consisted of performing serial (10-fold) dilutions of the tissue samples to a dilution of 10(-5). two selective media were used. l broth consisted of a basal brain heart infusion broth containing 5% horse serum, 5% yeast extract, and 100 micrograms of nad and 0.5 microgram of lincomycin per ml. l-b broth was identical to l broth, except 1.5 microgram of ba ... | 1983 | 6350343 |
| long-acting oxytetracycline for control of induced pasteurella multocida rhinitis in swine. | a long-acting oxytetracycline formulation was evaluated for control of rhinitis induced experimentally in pigs with a capsular type a, toxin-negative, low-passage strain of pasteurella multocida. the pigs were 6 to 7 weeks old and were naturally infected with haemophilus parasuis. the h parasuis infection was thought to predispose to establishment of p multocida in the nasal cavity. a long-acting oxytetracycline formulation was given im at the rate of 20 mg/kg, 4 times at 5-day intervals. medica ... | 1983 | 6618971 |
| haemophilus parasuis septicaemia in pigs. | | 1983 | 6626067 |
| effect of temperature on the survival of haemophilus parasuis in physiological saline. | the survival of haemophilus parasuis in physiological saline and in sodium phosphate buffered saline (pbs) at various temperatures was investigated and compared with that of h. pleuropneumoniae, pasteurella multocida, and escherichia coli. when placed in physiological saline, h. parasuis became undetectable at 42 degrees c within one hour, at 37 degrees c within 2 hours, and at 25 degrees c within 8 hours, but decreased slightly in amount at 5 degrees c for 8 hours. it showed the same tendency w ... | 1982 | 6752729 |
| experimental infections of mice and guinea pigs with haemophilus parasuis. | the pathogenicity of haemophilus parasuis for mice and guinea pigs was examined. mice were inoculated intraperitoneally with 2 x 10(7) to 2 x 10(9) organisms suspended in saline or in broth containing fresh yeast extract. most of them survived after inoculation. death occurred only in mice inoculated with 2 x 10(9) organisms suspended in broth. the recovery rate of h. parasuis from all the dead mice varied from 28.9% of the brains to 71.4% of the lungs. there were no lesions observed in any mous ... | 1982 | 7078659 |
| tylosin tartrate and tiamutilin effects on experimental piglet pneumonia induced with pneumonic pig lung homogenate containing mycoplasmas, bacteria and viruses. | the effects of tylosin tartrate and tiamutilin were examined in pneumonias induced experimentally in neonatal piglets with a homogenate of pneumonic pig lung, obtained from pigs with naturally acquired enzootic pneumonia. the homogenate contained mycoplasmas, including mycoplasma hyopneumoniae (m suipneumoniae) and m hyorhinis, and certain bacteria and viruses. the experimental pneumonias generally resembled mycoplasmal pneumonia histologically but were complicated by aspiration pneumonia in som ... | 1982 | 7134653 |
| [bacteriological diagnosis of haemophilus pleuropneumonia in swine]. | haemophilus pleuropneumonia (matthews and pattison, 1961; shope, 1964) should now be accepted as the valid name of the pathogen of hemophilus pleuropneumoniae of swine, according to studies and the description by kilian and co-workers (1978), while the name of haemophilus parahaemolyticus should be considered a synonym. on top of haemophilus pleuropneumoniae, haemophilus parasuis and haemophilus spec. "minor group" are also important to pneumonia, serositis, and arthritis (glässer's disease) in ... | 1981 | 7197147 |
| glässer's disease in piglets produced by intraperitoneal inoculation with haemophilus parasuis. | haemophilus parasuis was inoculated into the abdominal cavities of piglets. its infective doses varied from 1.4 x 10(6) to 1.0 x 10(9) organisms. when inoculated with 1.0 x 10(9) or 1.6 x 10(8) organisms, piglets were affected with fibrinous polyserositis associated with arthritis and purulent meningitis. the three disorders were considered to be characteristic of glässer's disease. septicemia was also found in many of the infected piglets, some of which died within a few days after inoculation. ... | 1981 | 7335122 |
| prevention of glässer's disease through immunity to haemophilus parasuis. | | 1981 | 7336832 |
| isolation of the major outer-membrane protein of actinobacillus pleuropneumoniae and haemophilus parasuis. | a polyclonal antibody against the 35 kda major outer-membrane protein of pasteurella multocida cross-reacted with the 40 kda major outer-membrane protein of actinobacillus pleuropneumoniae and the 42 kda major outer-membrane protein of haemophilus parasuis. the n-terminal amino-acid sequences of these proteins revealed a strong homology with the putative 35 kda porin protein of pasteurella multocida (66.7 and 76.2%, respectively). significant homologies were also evident between the 40 kda and t ... | 1995 | 7483902 |
| simplified procedure for preparation of sensitized latex particles to detect capsular polysaccharides: application to typing and diagnosis of actinobacillus pleuropneumoniae. | a novel, inexpensive method for obtaining immunoglobulin g (igg) specific for capsular antigen is described for use in latex agglutination tests. hyperimmune rabbit serum against encapsulated actinobacillus pleuropneumoniae was thoroughly adsorbed with a nonencapsulated mutant. the capsule titer of the absorbed serum was unaffected, whereas reactivity to nonencapsulated cells was reduced to background levels, as determined by enzyme immunoassay. the igg component of the adsorbed serum was recove ... | 1995 | 7494018 |
| porcine reproductive and respiratory syndrome: clinical disease, pathology and immunosuppression. | porcine reproductive and respiratory syndrome (prrs) was first known as blue-eared pig disease in the united kingdom and the causative agent as 'lelystad virus'. the disease is characterised by very variable clinical signs, including reproductive failure and respiratory disease. the respiratory syndrome is often associated with severe infection with secondary bacterial agents including pasteurella multocida, haemophilus parasuis and streptococcus suis. however, some seropositive herds show no cl ... | 1995 | 7709569 |
| contact-dependent acquisition of transferrin-bound iron by two strains of haemophilus parasuis. | two strains of haemophilus parasuis, namely, the type strain (atcc 19417) and strain e751, were investigated with respect to iron acquisition. both strains produced iron-repressible outer membrane proteins and could acquire iron from porcine transferrin but not from porcine lactoferrin. neither strain used bovine transferrin, and human transferrin was used to only a very limited extent, if at all. in all cases, iron acquisition from transferrin required direct contact between the organisms and t ... | 1995 | 7728656 |
| pathologic observations of pigs intranasally inoculated with serovar 1, 4 and 5 of haemophilus parasuis using immunoperoxidase method. | nineteen, 7- to 13-week-old pigs were inoculated intranasally with different strains of haemophilus parasuis (serovar 1, 4 and 5), and the pathological lesions induced by each strain were compared. eleven of thirteen pigs inoculated with either strain nagasaki (serovar 5) or no. 4 (serovar 1) died between days 1 to 6 after inoculation, and had septicemic lesions, meningitis, or polyserositis. one of six pigs inoculated with strain sw124 (serovar 4) died with polyserositis, another one recovered ... | 1994 | 7999883 |
| pathogenicity and immunity studies of haemophilus parasuis serotypes. | after intranasal inoculation of spf pigs with the 7 reference strains of haemophilus parasuis only the reference strains of serotypes 1 and 5 proved to be pathogenic and were able to produce the lesions characteristic of glässer's disease. even though the reference strains of serotypes 2, 3, 4, 6 and 7 proved to be apathogenic, intranasal inoculation resulted in the development of circulating antibodies as demonstrated by the complement fixation test. this serological response suggests an antige ... | 1993 | 8266897 |
| optimalization of the detection of nad dependent pasteurellaceae from the respiratory tract of slaughterhouse pigs. | nad dependent members of the family pasteurellaceae were cultured from the nasal cavity, surface and cut surface of the tonsils, and from the apical and caudal lobes of the lungs of 303 slaughterhouse pigs from 5 different herds in order to obtain information on the ecology of these bacteria. the specimens were plated on two different selective agar media using a special dilution technique that resulted in a good separation of individual colonies. bacteriological results were compared with serol ... | 1993 | 8273273 |
| [the effectiveness of tilmicosin in respiratory diseases of swine]. | within the scope of the clinical evaluation of tilmicosin in enzootic pneumonia of pigs, nasal swabs from 78 animals were taken, before and after oral medication of different doses (0, 100, 200, 300 mg tilmicosin/kg dry food), and examined bacteriologically for pasteurella multocida, bordetella bronchiseptica und haemophilus parasuis. the bacteria aforementioned were isolated from the nasal secretions of 83% of the pigs, 14 days after crowding without any prophylactic regime. it could be demonst ... | 1993 | 8431202 |
| experimental reproduction of haemophilus parasuis infection in swine: clinical, bacteriological, and morphologic findings. | haemophilus parasuis is a common cause of polyserositis and polyarthritis in swine. little is known about the mucosal and systemic sites of replication and lesions which follow an aerosol exposure to h. parasuis. in this experiment 5-week-old cesarean-derived, colostrum-deprived (cdcd) pigs were inoculated intranasally with an inoculum containing 2 x 10(9) colony-forming units of h. parasuis. two principals and one control pig were necropsied at 12, 36, 84, and 108 hours postinoculation (pi) and ... | 1995 | 8580168 |
| serological characterisation of haemophilus parasuis isolates from australian pigs. | a total of 31 isolates of haemophilus parasuis obtained from australian pigs were serotyped by the kielstein-rapp-gabrielson scheme. the isolates were assigned to serovar 1 (1 isolate), serovar 2 (1 isolate), serovar 4 (4 isolates), serovar 5 (7 isolates), serovar 9 (2 isolates), serovar 10/7 (4 isolates), serovar 12 (1 isolate) and serovar 13 (6 isolates). the remaining 5 isolates could not be assigned to a serovar. two different serovars (5 and 13) were detected in one herd. the only 2 isolate ... | 1996 | 8660221 |
| importance of staphylococcus hyicus ssp hyicus as a cause of arthritis in pigs up to 12 weeks of age. | lame pigs, up to 12 weeks of age, were necropsied to establish a diagnosis. of 175 pigs examined, 165 were confirmed to have arthritis by histopathological examination of joint tissues. lesions were most common in the elbow and tarsal joints and least common in the joints of the feet. typically, there was severe fibrinopurulent inflammation of synovial membranes regardless of the bacteria isolated. a bacterial aetiology was found in 114 (69%) of the 165 pigs. in arthritic pigs in which an aetiol ... | 1996 | 8660240 |
| mycoplasma hyorhinis infection levels in lungs of piglets with porcine reproductive and respiratory syndrome (prrs). | the infection levels of mycoplasma hyorhinis, m. hyopneumoniae and m. hyosynoviae in the lung of piglets were examined in relation to porcine reproductive and respiratory syndrome (prrs). these animals consisted of 43 prrs piglets with prrs, 2 piglets infected with prrs virus but symptom-free, and 10 control piglets free of prrs virus and its antibody. m. hyorhinis was isolated from 40 of the 43 prrs piglets, from 1 of the 2 latent infected piglets and from 3 of the 10 control piglets. the numbe ... | 1996 | 8672579 |
| detection of strain-specific antigenic epitopes on the lipo-oligosaccharide of haemophilus parasuis by use of monoclonal and polyclonal antibodies. | to investigate the antigenic diversity of lipo-oligosaccharides of haemophilus parasuis. | 1996 | 8720240 |
| characterisation of porcine haemophili isolated from australian pigs between 1988 and 1992. | a total of 362 haemophili, isolated pigs throughout australia, were characterised by phenotypic properties. most were identified as actinobacillus pleuro-pneumoniae (296 isolates) or haemophilus parasuis (52 isolates). the remaining isolates were identified as haemophilus taxon 'minor group' (12 isolates) and haemophilus taxon d (two isolates). all 296 a pleuropneumoniae isolates were serotyped by slide agglutination and/or gel diffusion, using rabbit antisera against all 12 recognised serovars. ... | 1995 | 8787520 |
| purification and partial characterization of a novel antibacterial agent (bac1829) produced by staphylococcus aureus ksi1829. | a novel antimicrobial agent from staphylococcus aureus ksi1829, designated bac1829, was purified by sequential steps of ammonium sulfate precipitation, sephadex g-50 gel filtration chromatography, and hydrophobic interaction chromatography. purified bac1829 has a molecular mass of 6,418 +/- 2 da. the peptide in heat stable, since full biological activity is retained after heating at 95 degrees c for 15 min, and it is destroyed by digestion with proteases. amino acid sequence analysis revealed a ... | 1996 | 8795206 |
| pathogenicity of haemophilus parasuis serovars 4 and 5 in contact-exposed pigs. | the pathogenicities of haemophilus parasuis strains sw124 (serovar 4) and nagasaki (serovar 5) were examined by contact-exposure of specific pathogen-free (spf) pigs. ten pigs were divided into three groups. two of four pigs in the first group were inoculated intranasally (in) with 2 x 10(8) cfu of strain sw124, and the other two pigs were mingled with these in-exposed ones. all the four pigs were subclinically infected in this group. the four pigs of the second group were likewise exposed to st ... | 1996 | 8811627 |
| evaluation of a pcr for detection of actinobacillus pleuropneumoniae in mixed bacterial cultures from tonsils. | a pcr for the detection of actinobacillus pleuropneumoniae was evaluated. all of 102 field, isolates of a, pleuropneumoniae reacted in the pcr by amplification of a 985 bp product. no pcr amplification product was observed when examining strains of a. ureae, a. capsulatus, a. hominis, a. equuli, a, rossii, a. suis, escherichia coli, bordetella bronchiseptica. streptococcus suis, pasteurella haemolytica, pasteurella multocida, haemophilus parasuis, haemophilus taxon minor group, haemophilus taxon ... | 1996 | 8828126 |
| general overview of prrsv: a perspective from the united states. | four years after the report of its discovery, porcine reproductive and respiratory syndrome virus (prrsv) continues to challenge swine producers, veterinary practitioners, and animal health researchers in the united states. the prevalence of infection is high--60% to 80% of herds is a reasonable estimate--but the clinical effects of infection vary widely among farms. in many herds, infection is unapparent and productivity seemingly unaffected. some infected herds report occasional respiratory di ... | 1997 | 9220613 |
| neuraminidase (sialidase) activity of haemophilus parasuis. | neuraminidase (sialidase), a potential virulence factor in bacteria, was demonstrated in haemophilus parasuis, an invasive swine pathogen, but not in four other pathogens of the pasteurellaceae family: h. influenzae, h. somnus, h. paragallinarum, or actinobacillus pleuropneumoniae. h. parasuis neuraminidase had an acidic ph optimum and a specificity for several substrates also cleaved by other bacterial neuraminidases. similar to the neuraminidase of pasteurella multocida, h. parasuis neuraminid ... | 1997 | 9231420 |
| effects on endotoxin pathogenicity in pigs with acute septicemia of haemophilus parasuis infection. | changes of endotoxin in plasma, and the response of the coagulation system and blood cells in septicemia of haemophilus parasuis infection were examined by inoculation with h. parasuis in specific pathogen-free (spf) pigs. eight pigs were inoculated intratracheally with 10(5), 10(6) and 10(7) colony formation units (cfu) of the strain nagasaki (serovar 5). all pigs died 28 to 42 hr after inoculation. haematologically, severe leukopenia occurred 24 hr post inoculation (hpi) until death. glucose c ... | 1997 | 9234219 |
| interaction of haemophilus parasuis with nasal and tracheal mucosa following intranasal inoculation of cesarean derived colostrum deprived (cdcd) swine. | twenty-three cesarean derived, colostrum deprived pigs were obtained at 5 wk of age and inoculated intranasally with either 1.4 x 10(8) colony forming units of haemophilus parasuis or sterile phosphate buffered saline. pigs were euthanized at 4, 8, 12, 18, 26, or 36 h post-inoculation and tissues from the oropharynx and respiratory tract were obtained for qualitative bacterial culture, immunohistochemistry for h. parasuis antigens, and light and transmission electron microscopy. haemophilus para ... | 1997 | 9243000 |
| purification and kinetic characterization of haemophilus parasuis malate dehydrogenase. | haemophilus parasuis malate dehydrogenase ((s)-malate:nad+ oxidoreductase; ec 1.1.1.37) isolated from cell sonicates was purified 584-fold to electrophoretic homogeneity with a 19% recovery and a specific activity of 222 units/mg protein. sds-polyacrylamide gel electrophoresis and molecular exclusion chromatography indicated the purified enzyme to be a dimer composed of 34,600 molecular weight subunits. kinetic parameters for all four substrates in the forward and reverse reactions indicated a s ... | 1997 | 9244395 |
| immunohistochemical detection of haemophilus parasuis serovar 5 in formalin-fixed, paraffin-embedded tissues of experimentally infected swine. | an avidin-biotin complex immunohistochemistry technique was developed to detect haemophilus parasuis serovar 5 in experimentally infected 18-21-day-old conventional pigs, using a rabbit polyclonal antiserum. seven of 10 intratracheally inoculated animals developed a low to medium degree of fibrinous polyserositis; meninges and pleura were the most severely affected areas. haemophilus parasuis was recovered from 9 of 10 pigs; in 2 of them h. parasuis was isolated from tracheal swabs only. positiv ... | 1997 | 9249161 |
| [examination of primary spf swine after experimental infection with haemophilus parasuis. clinical symptoms, changes in hematological parameters and in the parameters of the cerebrospinal fluid]. | the objectives of this work were to cause the glässer's disease (gd) in primary specific pathogen free piglets after experimental infection, to observe the clinical symptoms and to examine the influence of the infection on the haematological parameters. gd was caused by experimental infection of haemophilus parasuis in seven to eight weeks old specific pathogen free piglets. in relation to the infection route the morbidity was high (83-100%) and 20% of the infected piglets died. based on the phy ... | 1997 | 9289881 |
| cleavage of influenza a virus h1 hemagglutinin by swine respiratory bacterial proteases. | cleavage of influenza a virus hemagglutinin (ha) is required for expression of fusion activity and virus entry into cells. extracellular proteases are responsible for the proteolytic cleavage activation of avirulent avian and mammalian influenza viruses and contribute to pathogenicity and tissue tropism. the relative contributions of host and microbial proteases to cleavage activation in natural infection remain to be established. we examined 23 respiratory bacterial pathogens and 150 aerobic ba ... | 1997 | 9311838 |
| association of tumour necrosis factor and acute phase reactant changes with post arrival disease in swine. | severe weight loss in the absence of respiratory, enteric or systemic clinical disease or gross pathologic lesions is often observed when immunologically naive boars are placed in conventional health swine facilities. affected animals develop this weight loss in spite of receiving pre-entry vaccinations against common swine pathogens, such as haemophilus parasuis or mycoplasma hyopneumoniae. in many cases, the weight loss is non-responsive to long term antibiotic therapy. in order to determine t ... | 1997 | 9323928 |
| [bronchoalveolar lavage on pig breeding farms]. | in 182 pigs lung lavage was performed using a endotracheal tube and a catheter. the collected bronchoalveolar lavage fluid (balf) was examined microbiologically. with decreasing numbers alpha-hämolytic streptococci, bordetella bronchiseptica, haemophilus parasuis, pasteurella multocida were cultured. actinobacillus pleuropneumoniae was isolated from 3 balfs. in one farm piglets were lavaged routinely for monitoring of the lung health status. | 1997 | 9410726 |
| ultrastructural study of porcine alveolar macrophages infected in vitro with porcine reproductive and respiratory syndrome (prrs) virus, with and without haemophilus parasuis. | two experiments were designed to study ultrastructural changes in porcine alveolar macrophages (pam) inoculated with porcine reproductive and respiratory syndrome (prrs) virus (experiment 1) and with prrs virus and haemophilus parasuis (experiment 2). in both experiments, the viral infectious dose represented a "multiplicity of infection" of 1. viral infection alone induced minimal ultrastructural changes at this dose, consisting only of an increase in lysosome numbers. mixed viral and bacterial ... | 1998 | 9595354 |
| inducible nitric oxide synthase expression in porcine immune cells. | porcine immune cells were examined for the ability to produce inducible nitric oxide synthase following in vitro or in vivo stimulation. enzyme activity and product formation were not detected following stimulation of porcine peripheral blood mononuclear cells (pbmc), splenocytes, or alveolar macrophages with a combination of cona and lipopolysaccharide (lps) or recombinant porcine interferon gamma and lps. in vitro engulfment of haemophilus parasuis by macrophages also failed to induce inducibl ... | 1998 | 9613441 |
| [occurrence of bacterial infectious agents in pathologically/anatomically altered lungs of pigs and compilation of resistance spectra]. | the goal of this work was to investigate the type and etiology of bacterial porcine pneumonia by analyses of autopsy findings made in the tierärztlichen ambulanz schwarzenbek, aussenstelle der freien universität berlin, and in the institut für tiergesundheit, milchhygiene und lebensmittelqualität der landwirtschaftskammer westfalen-lippe in münster in the years from 1991 to 1993. the evaluation of the results of the total 6560 autopsies (n = 6560) of pigs shows: 1. pneumonia as the main diagnosi ... | 1998 | 9639952 |
| [determination of c-reactive protein and neopterin in serum of diseased and bacterially infected swine]. | the parameters c-reactive protein (crp) and neopterin, which are associated with immunological reactions, were investigated in serum of healthy, diseased and with haemophilus parasuis infected pigs. when comparing diseased young pigs with healthy young pigs significant increases of the crp- and neopterin concentrations can be seen. the increase of the crp-concentration was most remarkable. after the infection of spf-piglets with haemophilus parasuis, significantly decreased neopterin concentrati ... | 1998 | 9789368 |
| effect of porcine reproductive and respiratory syndrome virus infection on the clearance of haemophilus parasuis by porcine alveolar macrophages. | porcine reproductive and respiratory syndrome virus (prrsv) infection in young piglets is frequently associated with secondary infection due to various pathogens, especially those of the respiratory tract. one of the most important mechanisms in respiratory diseases is related to the alteration of function of porcine alveolar macrophages (pams). the objective of this study was to determine how prrs virus infection affects the capabilities of pams in the phagocytosis and destruction of haemophilu ... | 1998 | 9798089 |
| experimental infection of maternally immune pigs with porcine reproductive and respiratory syndrome (prrs) virus. | to investigate the influence of maternal antibody to porcine reproductive and respiratory syndrome (prrs) virus infection, the following examination was done using conventional and spf pigs. ten 17-day-old conventional pigs with maternal antibody against prrs virus and 6 44-day-old spf pigs seronegative were inoculated intranasally with 10(5.0) tcid50 of prrs virus. two conventional and 4 spf pigs were served as non-inoculated control. in conventional pigs, coughing and febrile response were obs ... | 1998 | 9879527 |
| protective role of maternal antibodies against haemophilus parasuis infection. | to evaluate the impact of maternal antibodies after challenge exposure of baby pigs with a homologous serovar of haemophilus parasuis. | 1999 | 9918152 |
| porcine reproductive and respiratory syndrome virus and haemophilus parasuis antigen distribution in dually infected pigs. | immunohistochemical, viral and bacterial isolation techniques were used to study the distribution and localization of porcine reproductive and respiratory syndrome virus (prrsv) and haemophilus (h.) parasuis in experimentally infected pigs. thirty pigs seronegative to prrsv and h. parasuis were divided into four groups. group a pigs (10 animals) were inoculated with both virus and bacteria; group b pigs (10 animals) were inoculated with bacteria, group c pigs (five animals) were inoculated with ... | 1999 | 10063534 |
| development of an oligonucleotide-specific capture plate hybridization assay for detection of haemophilus parasuis. | an oligonucleotide-specific capture plate hybridization assay has been developed to rapidly, specifically, and sensitively detect haemophilus parasuis from nasal swabs. several in vitro studies have been performed to determine the sensitivity and specificity of the test, and in vivo studies have validated this technique in pigs. results suggest that the assay detects <100 colony-forming units/ml in a pure culture and gives a positive result when h. parasuis is present in a ratio of 1:10(3)-10(4) ... | 1999 | 10098685 |
| prevalence of haemophilus parasuis serovars isolated in spain from 1993 to 1997. | from 1993 to 1997, 327 strains of haemophilus parasuis were isolated from spanish swine in our diagnostic laboratory and 174 strains (53.2%) were serotyped. four serotypes, sv. 5 (18.4%), sv 4 (16%), sv. 2 (9.2%) and sv. 13 (8%) were the most frequently isolated and 29.3% of the studied strains were classified as non typable. the results obtained indicate that the distribution of the serotypes in spain is very similar to that found by other researchers in germany, australia, canada and alike to ... | 1999 | 10227126 |
| biological activities of lipopolysaccharides extracted from porcine vaccine strains. | lipopolysaccharides (lpss) were purified from actinobacillus pleuropneumoniae serotype 2, bordetella bronchiseptica and haemophilus parasuis serotype 5, which were used for vaccine production in japan, by the phenol-water procedure. in sds-page analysis, a. pleuropneumoniae lps, as well as escherichia coli lps, demonstrated a typical ladder profile of a smooth-type lps. on the other hand, b. bronchiseptica and h. parasuis lpss lacked the ladder profiles. it was found that the biological activity ... | 1999 | 10651044 |
| a 24-kda cloned zinc metalloprotease from actinobacillus pleuropneumoniae is common to all serotypes and cleaves actin in vitro. | actinobacillus pleuropneumoniae causes pleuropneumonia in swine. this bacterium secretes proteases that degrade porcine hemoglobin and iga in vitro. to further characterize a. pleuropneumoniae proteases, we constructed a genomic library expressed in escherichia coli dh5alpha, and selected a clone that showed proteolytic activity. the recombinant plasmid carries an 800-base pair a. pleuropneumoniae gene sequence that.codes for a 24-kda polypeptide. a 350-base pair psti fragment from the sequence ... | 2000 | 10805246 |
| apx toxins in pasteurellaceae species from animals. | pasteurellaceae species particularly of porcine origin which are closely related to actinobacillus pleuropneumoniae were analyzed for the presence of analogues to the major a. pleuropneumoniae rtx toxin genes, apxicabd, apxiica and apxiiicabd and for their expression. actinobacillus suis contains both apxicabd(var.suis) and apxiica(var. suis) operons and was shown to produce apxi and apxii toxin. actinobacillus rossii contained the operons apxiica(var.rossii) and apxiiicabd(var.rossii). however, ... | 2000 | 10831858 |
| establishment, validation and use of the kielstein-rapp-gabrielson serotyping scheme for haemophilus parasuis. | to produce antisera to the 15 recognised reference strains of the kielstein-rapp-gabrielson (krg) serotyping scheme for haemophilus parasuis, validate those sera and use them to serotype 46 australian field isolates of h parasuis. | 2000 | 10860155 |
| in vitro susceptibility of porcine respiratory pathogens to tilmicosin. | bacterial isolates obtained from swine with various clinical diseases were tested for susceptibility to tilmicosin by minimum inhibitory concentration (mic) and kirby-bauer disk diffusion tests using national committee on clinical laboratory standards methodology. the tilmicosin mic90 was < or =0.125 microg/ml for erysiopelothrix rhusiopathiae, < or = 1 microg/ml for haemophilus parasuis isolates, 8 microg/ml for actinobacillus suis and pasteurella multocida type a, 16 microg/ml for toxigenic an ... | 2000 | 11108454 |
| application of eric-pcr for the comparison of isolates of haemophilus parasuis. | to validate a polymerase chain reaction (pcr) based method. enterobacterial repetitive intergenic consensus-pcr (eric-pcr), for the fingerprinting of haemophilus parasuis strains and to use that method to differentiate isolates from apparently related outbreaks of glässer's disease on three pig farms. | 2000 | 11194474 |
| outer membrane proteins and dna profiles in strains of haemophilus parasuis recovered from systemic and respiratory sites. | polyserositis caused by haemophilus parasuis is an important disease that affects mostly weaned pigs. recent studies have shown that virulence can differ among strains recovered from distinct body sites and also that it may be related to the presence of certain outer membrane proteins (omps). the objective of this study was to compare the omp and dna profiles of h. parasuis strains isolated from systemic and respiratory sites from diseased and healthy pigs. strains evaluated in this study were p ... | 2001 | 11325986 |
| the transferrin receptor of actinobacillus pleuropneumoniae: quantitation of expression and structural characterization using a peptide-specific monoclonal antibody. | when actinobacillus pleuropneumoniae (a. pp) is grown under iron-restricted conditions in vitro, transferrin binding proteins (tbps) are induced. the functional transferrin receptor of a. pp is composed of two outer membrane proteins (tbp1 and tbp2) and shows an exquisite specificity for porcine transferrin. this complex was studied using a monoclonal antibody (mab 1.48) raised against a synthetic peptide corresponding to a hydrophilic domain of tbp2 common to several a. pp serotypes. the antibo ... | 2001 | 11356318 |
| phenotypic and genetic characterization of nad-dependent pasteurellaceae from the respiratory tract of pigs and their possible pathogenetic importance. | nicotinamide adenine dinucleotide (nad)-dependent pasteurellaceae other than actinobacillus pleuropneumoniae and haemophilus parasuis are frequently isolated from the respiratory tract of pigs. the taxonomic classification and relevance for pathogenicity of these bacteria deserves further attention. in the present study, 107 of these nad-dependent isolates from the porcine respiratory tract, primarily from lungs with pathological changes, were investigated. on the basis of phenotypic criteria, s ... | 2001 | 11390108 |
| a cross-protection experiment in pigs vaccinated with haemophilus parasuis serovars 2 and 5 bacterins, and evaluation of a bivalent vaccine under laboratory and field conditions. | cross-protection between haemophilus parasuis serovars 2 and 5 was examined in pigs using a bacterin based vaccine, and subsequently the safety and efficacy of a bivalent vaccine were evaluated. upon intratracheal challenge of a serovar 2 or 5 strain, pigs immunized with a monovalent vaccine were protected against challenge with a homologous serovar strain, but not with a heterologous serovar strain. immunization with a bivalent vaccine containing both serovars 2 and 5 bacterins conferred protec ... | 2001 | 11411491 |
| [the current antimicrobial resistance situation in swiss veterinary medicine]. | antimicrobial susceptibility data (n = 1501) and bacterial isolates (n = 258) of important bacterial pathogens from animals were collected in collaboration with eight swiss laboratories from may 1999 to february 2000. using these data, the antimicrobial resistance situation could be assessed for the following bacterial species: escherichia coli, salmonella, haemophilus parasuis, actinobacillus pleuropneumoniae, pasteurella multocida, mannheimia haemolytica, bordetella bronchiseptica, campylobact ... | 2001 | 11680911 |
| development of a pcr test to diagnose haemophilus parasuis infections. | a polymerase chain reaction (pcr) test was developed in order to improve the accuracy and speed of diagnosis of haemophilus parasuis, an economically important respiratory pathogen that affects swine. the gene sequence of the 16s small subunit ribosomal rna of h. parasuis (genbank m75065) was compared with 56 16s sequences of related bacteria, including those frequently isolated from pig tissues. two species-specific primers were designed: hps forward and hps reverse. the predicted size of the a ... | 2001 | 11724140 |
| the effect of a homologous bacterin given to sows prefarrowing on the development of glässer's disease in postweaning pigs after i.v. challenge with haemophilus parasuis serotype 5. | the trial was carried out to evaluate the impact of maternal antibodies on the development of glässer's disease after i.v. exposure of weaned pigs with a homologous serovar of haemophilus parasuis (hps). two groups of weaned pigs were formed. group one vi (n = 10): born to vaccinated sows, weaners i.v. challenged one week postweaning and euthanatized 14 days postweaning. group two nvi (n = 10 wearners): born to non vaccinated sows, i.v. challenged one week postweaning euthanatized 14 days postwe ... | 2002 | 12125173 |
| effect of vaccinating sows and their piglets on the development of glässer's disease induced by a virulent strain of haemophilus parasuis serovar 5. | ten pregnant gilts were divided into two groups of five and one group was vaccinated at 80 and 95 days of pregnancy with a commercial bacterin containing haemophilus parasuis serovars 2, 3 and 5. half the piglets born to each group of gilts were vaccinated at seven and 21 days of age with the same bacterin, and one week after they were weaned at five weeks, all the piglets were inoculated intratracheally with 10(6) colony-forming units of hparasuis serovar 5. at slaughter, a significantly smalle ... | 2002 | 12137419 |
| infectious and rearing-system related risk factors for chronic pleuritis in slaughter pigs. | chronic pleuritis (cp) in danish pigs for slaughter is by far the most frequent finding at the routine post-mortem meat inspection. an initial investigation published in 1990 demonstrated infectious and management-related risk factors. serological testing for additional infectious agents, as well as the need to consider the effect of disease clustering at the herd level, required a re-analysis of the data. our re-analysis used a representative sample of 4,800 pigs originating from 623 danish her ... | 2002 | 12163250 |
| antibodies to selected viral and bacterial pathogens in european wild boars from southcentral spain. | serum samples from 78 european wild boars (sus scrofa) harvested during the 1999-2000 hunting season were tested for antibodies to brucella spp., classical swine fever virus, erysipelothrix rhusiopathiae, haemophilus parasuis, leptospira interrogans serovar pomona, mycoplasma hyopneumoniae, pseudorabies virus (prv), porcine parvovirus (ppv), porcine reproductive and respiratory syndrome virus, salmonella serogroups b, c, and d, streptococcus suis, and swine influenza virus (siv) serotypes h1n1 a ... | 2002 | 12238391 |
| protection of vaccinated pigs against experimental infections with homologous and heterologous haemophilus parasuis. | the efficacy of a new haemophilus parasuis vaccine for pigs was investigated. the vaccine contains h parasuis serotype 5 cells and is adjuvanted with diluvac forte (intervet). groups of pigs were vaccinated at five and seven weeks with 2 ml and their littermates served as unvaccinated controls. the vaccinated pigs were protected against a challenge with another strain of hparasuis serotype 5 at two, eight and 17 weeks after the second vaccination, whereas the controls became very ill. the suscep ... | 2002 | 12430998 |
| the effect of season and vaccination for glässer's disease and post-weaning colibacillosis in an outdoor pig unit endemically infected with virulent strain of haemophilus parasuis serotype 5 and pathogenic escherichia coli. | the objective of this field trial was to determine if vaccination against haemophilus parasuis serovar 5 (hps 5) and pathogenic serotypes of escherichia coli would improve nursery pig performance in an outdoor unit in different seasons. the unit was concurrently infected with hps 5 and with different serotypes of e. coli. all piglets were born to hps 5 vaccinated sows. the trial was carried out in four (two summer and two winter) groups. group 1 (e. coli and hps vaccinated, summer season) (n = 3 ... | 2002 | 12485355 |
| influences of naturally occurring and experimentally induced porcine pneumonia on blood parameters. | it had been the objective of the studies described to establish local and systemic changes by naturally occurring pneumonia or pneumonia experimentally induced by pasteurella multocida and haemophilus parasuis in swine. acute and chronic pneumonia was found to alter the cytokine level of lung lavage fluid and affect the composition and function of blood cells, especially with regard to phagocytosis, radical formation and cell surface receptors. interleukin-6 levels in blood plasma rose 24h after ... | 2003 | 12507563 |
| typing of haemophilus parasuis strains by pcr-rflp analysis of the tbpa gene. | on the basis of a species-specific pcr assay, a rflp analysis for typing of haemophilus parasuis strains was developed and evaluated. amplification was based on the gene tbpa, encoding a transferrin-binding protein. rflp analysis of the 1.9-kb tbpa-amplicon using taqi, avai and rsai endonucleases produced 12 different patterns for the reference strains of the 15 known h. parasuis serovars, and showed a high heterogeneity (33 rflp groups) for 101 h. parasuis clinical isolates tested. the sensitiv ... | 2003 | 12523987 |
| value of indirect hemagglutination and coagglutination tests for serotyping haemophilus parasuis. | an indirect hemagglutination test (iha) and a coagglutination test (ca) were evaluated using saline, boiled, and autoclaved extracts for serotyping haemophilus parasuis. ca showed several cross-reactions, whereas iha gave rise to specific reactions, with minor exceptions. iha was further compared with the immunodiffusion test (the "gold standard") for the serotyping of 67 field isolates. as a conclusion, iha is recommended as a useful method for sensitive and specific serotyping of h. parasuis. | 2003 | 12574306 |
| purification and renaturation of membrane neuraminidase from haemophilus parasuis. | haemophilus parasuis, which causes polyserositis, polysynovitis, meningitis, septicemia, and pneumonia in pigs, has emerged as an increasing problem in modern swine production systems. co-factors for and the pathogenesis of h. parasuis disease are not defined. one of the potential virulence factors of h. parasuis is its neuraminidase (sialidase). while purifying the h. parasuis neuraminidase from the membrane fraction, we developed a protocol to renature enzymatic activity after enzyme preparati ... | 2003 | 12591209 |
| characterization of the diversity of haemophilus parasuis field isolates by use of serotyping and genotyping. | to characterize the genetic diversity of haemophilus parasuis field isolates with regard to serovar, herd of origin, and site of isolation. | 2003 | 12693533 |
| naturally-farrowed, artificially-reared pigs as an alternative model for experimental infection by haemophilus parasuis. | the use of naturally-farrowed, artificially-reared piglets as an alternative model to study haemophilus parasuis infections was evaluated. two trials were performed in order to evaluate the proposed model. in trial 1, animals were vaccinated and challenged with h. parasuis. results showed that the proposed model was effectively used to evaluate protective immunity against this organism. in trial 2, animals were challenged with different doses of h. parasuis. results showed that the severity of c ... | 2003 | 12760482 |
| evaluation of survival of actinobacillus pleuropneumoniae and haemophilus parasuis in four liquid media and two swab specimen transport systems. | to determine duration and rates of recovery of actinobacillus pleuropneumoniae and haemophilus parasuis from 4 liquid media and 2 swab specimen transport systems and compare findings with those of escherichia coli. | 2003 | 13677398 |
| a search for virulence genes of haemophilus parasuis using differential display rt-pcr. | although haemophilus parasuis is an important bacterial pathogen of swine, little is known about its pathogenesis or why some strains seem to be more virulent than others. therefore, we used differential display reverse transcription-polymerase chain reaction (ddrt-pcr) to search for virulence-associated genes in a pathogenic serotype 5 strain, h. parasuis 1185. gene expression was evaluated following growth in conditions chosen to begin to approximate those found in the upper respiratory tract ... | 2003 | 14519336 |
| development of a new serological test for serotyping haemophilus parasuis isolates and determination of their prevalence in north america. | haemophilus parasuis causes polyserositis in swine. fifteen serovars have been characterized by immunodiffusion test, but many field strains are not typeable. isolates (n = 300) of h. parasuis from animals in north america were serotyped by a new indirect hemagglutination test. the test was rapid and effective for serotyping of h. parasuis, and serovars 4, 5, 13, and 7 were the most prevalent serotypes. | 2004 | 14766867 |
| haemophilus parasuis: new trends on diagnosis, epidemiology and control. | haemophilus parasuis is a commensal organism of the upper respiratory tract of conventional pigs, but under appropriate conditions can invade and cause severe systemic disease, characterized by fibrinous polyserositis, arthritis and meningitis. factors involved in systemic invasion by h. parasuis remain largely unknown. however, major advances in our knowledge of h. parasuis include (1) development of a species-specific pcr test to detect h. parasuis in clinical samples, (2) study of molecular e ... | 2004 | 15019107 |
| prior infection with bordetella bronchiseptica increases nasal colonization by haemophilus parasuis in swine. | the objective of this study was to determine whether bordetella bronchiseptica would predispose to colonization or disease with haemophilus parasuis. three experiments were completed. in the first experiment, three groups of pigs (10 pigs/group) were inoculated intranasally with either b. bronchiseptica, h. parasuis, or with b. bronchiseptica followed by h. parasuis 1 week later. a fourth group of 10 pigs served as a non-infected control group. the second experiment was like the first, except th ... | 2004 | 15019114 |
| antimicrobial susceptibility of haemophilus parasuis and histophilus somni from pigs and cattle in denmark. | a total of 52 haemophilus parasuis and 80 histophilus somni isolates were tested for antimicrobial susceptibility by mic-determinations. none of the isolates were resistant to ampicillin, ceftiofur, ciprofloxacin, erythromycin, florphenicol, penicillin, spectinomycin, tetracycline, tiamulin, or tilmicosin. two h. parasuis isolates were resistant to trimethoprim + sulfamethoxazole. six h. parasuis isolates had reduced susceptibility (0.06-0.5 microg/ml) to ciprofloxacin and 10 reduced susceptibil ... | 2004 | 15172697 |
| prevalence of haemophilus parasuis serotypes in large outdoor and indoor pig units in hungary/romania/serbia. | gross postmortem pathologic-anatomical and bacteriological examination, and serotyping for haemophilus parasuis were performed in 401 weaned pigs with the clinical symptoms of glässer's disease, originating from indoor (204 piglets out of 12 units) and outdoor (197 piglets out of 9 units) units in the country triangle hungary/romania/serbia. the majority of the isolates in indoor units included haemophilus parasuis serotypes 2, 5 and non typable, while in outdoor units, a high diversity (serotyp ... | 2004 | 15298053 |